咀嚼口香糖在护理领域的研究进展
作者：
王琛;王旋;许琍文;陈静;余涵;...
期刊：
中国临床护理 ,2022年14(01):59-62 ISSN：1674-3768
作者机构：
[王旋; 陈静; 陶龙珠; 余涵; 王琛] 430023 武汉,武汉轻工大学医学与健康学院;[许琍文] 430023 武汉,华中科技大学同济医学院附属武汉中心医院护理部
关键词：
咀嚼口香糖;假饲;护理;综述
摘要：
咀嚼口香糖是一种临床常用的假饲形式,文章综述咀嚼口香糖在护理领域中的应用及研究进展,为临床护理提供可行的、患者依从性较好的护理方法提供新思路和理论依据,以期为未来研究及临床应用提供借鉴和帮助。
语种：
中文
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糖尿病患者心理性胰岛素抵抗研究进展
作者：
陈静;王旋
期刊：
医药高职教育与现代护理 ,2022年5(4):369-372 ISSN：2096-501X
作者机构：
[王旋; 陈静] 430023 湖北 武汉,武汉轻工大学 医学与健康学院
关键词：
糖尿病;心理性胰岛素抵抗;研究进展;综述
摘要：
文章综述糖尿病患者心理性胰岛素抵抗的概念、评估工具、心理性胰岛素抵抗对患者产生的影响和干预措施.建议选取合适的评估工具,着重解决心理性胰岛素抵抗对患者产生的不良影响,采取系统有效的干预措施来缓解糖尿病患者的负面心理体验.
语种：
中文
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脂肪组织巨噬细胞与肥胖
作者：
杨梦莹;张红梅;王旋;易兰
期刊：
职业与健康 ,2021年37(08):1137-1140 ISSN：1004-1257
作者机构：
[易兰; 张红梅; 杨梦莹] 华中科技大学同济医学院附属武汉中心医院内分泌科;[王旋] 武汉轻工大学医学技术与护理学院
关键词：
脂肪组织;巨噬细胞;交感神经相关性巨噬细胞;肥胖
摘要：
肥胖是一种代谢性炎症反应性疾病,肥胖动物体内脂肪组织巨噬细胞含量明显增高,且极化表型由抗炎的M2型转化为促炎的M1型。近年来,研究者发现在与脂肪组织相连的交感神经周围存在一种特殊的巨噬细胞,即交感神经相关性巨噬细胞,具有摄取和分解去甲肾上腺素的作用,导致脂肪分解障碍引起肥胖。我们对脂肪组织巨噬细胞、交感神经相关性巨噬细胞的生物学特性及其与肥胖的联系进行了综述。
语种：
中文
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基于CNKI的胃癌患者照顾者研究的文献分析
作者：
陈俊华;王旋;胡龙霞;刘文斌
期刊：
鄂州大学学报 ,2020年27(05):109-112 ISSN：1008-9004
作者机构：
武汉轻工大学 医学技术与护理学院,湖北武汉430023;[刘文斌; 王旋; 陈俊华; 胡龙霞] 武汉轻工大学
关键词：
胃癌;照顾者;中国知网;文献分析
摘要：
目的:分析发表在中国知网（China National Knowledge Infrastructure,CNKI）上的关于我国胃癌患者照顾者研究的发展动态,为今后的深入研究提供参考。方法:以中国知网（CNKI）为数据来源,选择知网下的文献数据库,在输入检索条件下的"主题"字段输入"胃癌"或者"照顾者",在"篇名"字段输入"照顾者"或者"家属","主题"与"篇名"之间用"并且"连接,选择"模糊"词频进行检索,对检索到的第一作者为中国大陆的文献进行分析。结果:共检索出65篇文献,选择符合纳入条件的21篇文献进行研究分析。结论:来自全国各地的研究者都越来越关注胃癌患者的照顾者,其中以描述性研究居多。在今后的研究中应该进一步拓展相关研究的广度与深度,探讨某种合适又高效的干预方式对照顾者进行干预后以达到提高患者的生活质量、促进医患关系和谐的目的。
语种：
中文
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肌肉损伤与修复的研究进展
作者：
邓苏爱;郭雨露;周文静;陈俊华;胡龙霞;...
期刊：
武汉轻工大学学报 ,2020年39(02):27-34 ISSN：2095-7386
作者机构：
武汉轻工大学医学技术与护理学院,湖北武汉430023;[刘文斌; 王旋; 邓苏爱; 郭雨露; 陈俊华; 周文静; 胡龙霞] 武汉轻工大学
关键词：
肌肉;损伤;修复;基因治疗;细胞疗法
摘要：
肌肉是维持人体生命活动的重要物质.它是由肌原纤维相互包裹形成肌束,通过肌膜固定支持并保持一定的形态,继而肌钙蛋白和肌球蛋白控制粗肌丝和细肌丝相互滑动,使肌小节缩短从而控制肌肉的收缩来维持人体的运动.肌肉损伤一般发生于外力作用下的挫伤或间接外力作用下的拉伤,但其他如生物、化学因素也可以导致肌肉损伤.肌肉损伤在一定程度上可以修复.目前,肌肉损伤与修复相关的研究已经向组织的超微结构、分子机制和基因水平拓展,主要致力于研究如何从细胞和基因水平上促进肌肉的再生,以及寻找安全有效的生物替代物来修复受伤的肌肉组织.本综述介绍了中外学者对肌肉结构、功能的认识,重点阐述了肌肉损伤后引发炎症反应,卫星细胞被激活促进肌纤维再生,以及这一系列过程中众多基因调控和蛋白的参与的分子机制,为后续研究提供参考.
语种：
中文
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新型冠状病毒感染的流行、医院感染及心理预防
作者：
王琛;王旋
期刊：
全科护理 ,2020年18(03):309-310 ISSN：1674-4748
作者机构：
[王旋; 王琛] 武汉轻工大学;武汉轻工大学医学技术与护理学院
关键词：
2019新型冠状病毒;肺炎;流行病学;医院感染;心理预防
摘要：
呼吸道传染病的流行病学及预防控制工作一直是医学界关注的热点问题。2019年12月,我国湖北省武汉市发生不明原因的肺炎疫情,武汉市成为全球关注的焦点。目前新型冠状病毒感染的肺炎尚在疫情发病的初期,发病还存在较强的不确定性,尚无特异性治疗。目前人类已知的冠状病毒,根据病毒的血清学特点和核苷酸序列分类分为α、β、γ和δ属,引起人感染的冠状病毒有HCoV-229E、HCoV-OC43、SARS-CoV、HCoV-NL63、HCoV-HKU1和MERS-CoV 6种[1]。
语种：
中文
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舒适护理评价指标的应用及研究进展
作者：
王利娟;刘冬梅;李侠;王旋
期刊：
全科护理 ,2020年(36):5073-5077 ISSN：1674-4748
作者机构：
临泉县人民医院;武汉轻工大学医学技术与护理学院
关键词：
舒适护理;评价指标;综述
摘要：
综述舒适护理的相关概念、舒适护理评价指标的国内外研究现状,整理分析舒适护理评价指标的临床应用,提出了国内外的差异性,以期为我国学者制定和选择合适的评价指标提供参考。
语种：
中文
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Targeted Antagonism of Vascular Endothelial Growth Factor Reduces Mortality of Mice with Acute Respiratory Distress Syndrome
作者：
Zhang, Zhao;Lu, Dong-shi;Zhang, Dan-qing;Wang, Xin;Ming, Yu* ;...
期刊：
当代医学科学(英文) ,2020年40(4):671-676 ISSN：2096-5230
通讯作者：
Wu, Zhou-yang;Ming, Yu
作者机构：
[Lu, Dong-shi; Wu, Zhou-yang; Zhang, Dan-qing; Zhang, Zhao] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Anesthesiol, Wuhan 430022, Peoples R China.;[Wang, Xin] Chinese Peoples Liberat Army, Dept Senior Cadre, 989 Hosp, Joint Logist Support Force, Luoyang 471003, Peoples R China.;[Ming, Yu] Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430032, Peoples R China.
通讯机构：
[Wu, Zhou-yang] H;[Ming, Yu] W;Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Anesthesiol, Wuhan 430022, Peoples R China.;Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430032, Peoples R China.
关键词：
acute respiratory distress syndrome;vascular endothelial growth factor;soluble fms-like tyrosine kinase-1;ultrasound microbubble;lipopolysaccharide
摘要：
Acute respiratory distress syndrome (ARDS) is associated with a mortality of 45%. Our previous research indicated that anti-vascular endothelial growth factor (VEGF) could maintain the normal structure and function of the respiratory barrier. However, systemic application of VEGF antagonists would lead to animal death. This study attempts to study the targeted drug delivery for ARDS. In this study, we used soluble fms-like tyrosine kinase-1 (sFlt)-targeted ultrasound microbubbles to antagonize the effect of VEGF on lung tissue. Ninety male BALB/c mice were randomly assigned to 6 groups: phosphate buffer saline (PBS) group (PBS+PBS); blank group (PBS+empty microbubbles); lipopolysaccharide (LPS) group (LPS+PBS); ARDS group (LPS+empty microbubbles); control group (PBS+sFlt microbubbles); and treatment group (LPS+sFlt microbubbles). After administration of LPS or PBS in the corresponding groups, the sFlt-targeted microbubbles or empty microbubbles were injected into the blood circulation. Then the lungs were irradiated with ultrasound, which ruptured the drug-loaded microbubbles and helped release drugs to the lung tissues targeted. The lung injury score, lung wet/dry ratio (W/D), liver and kidney functions, and the mortality of the mice in all groups were investigated at the predetermined time point. The difference in mortality between groups was examined by Fisher test. Other data were analyzed by one-way analysis of variance (ANOVA). A value of P<0.05 indicates that the difference was significant. The results showed that the PaO2 levels were normal in the PBS group, the blank group, and the control group. The LPS group and ARDS group showed significant hypoxia. PaO2 was improved significantly in the treatment group. The lung injury score and W/D were normal in the PBS group, the blank group, and the control group. The lung injury score and W/D increased significantly in the LPS group and ARDS group and decreased in the treatment group (P<0.05). The mortality rate of the ARDS model was 60% (95% confidence interval 47.5%–72.5%), and that with sFlt-targeted microbubbles was significantly lower at only 40% (95% confidence interval 27.5%–52.5%, P<0.05). It was concluded that anti-VEGF with sFlt targeted ultrasound microbubbles attenuated the lung injury and ultimately reduced the 7-day mortality effectively. It might be a suitable therapeutic tool for the treatment of ARDS. © 2020, Huazhong University of Science and Technology.
语种：
英文
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强化一维持健康教育对冠心病患者自我管理影响的研究
作者：
望家琴;王旋;杨梦莹;李应
期刊：
护士进修杂志 ,2019年34(9):777-780 ISSN：1002-6975
作者机构：
武汉轻工大学医学技术与护理学院,湖北武汉,430023;[王旋; 杨梦莹; 望家琴; 李应] 武汉轻工大学
关键词：
冠心病;健康教育;知识水平;自我管理;再入院率;强化-维持
摘要：
目的 探讨强化-维持健康教育对社区冠心病患者疾病相关知识水平、自我管理、再入院率的影响.方法 2017年3-12月采用方便抽样法选取武汉市某社区冠心病患者60例为研究对象,对其实施强化-维持健康教育,以冠心病相关知识问卷评价患者知识水平,冠心病自我管理行为量表(CSMS)评估患者自我管理能力,监测患者再入院率.结果 强化-维持干预后患者疾病相关知识总分及各维度得分明显高于干预前(P＜0.05),干预后冠心病相关知识问卷中诱发因素、药物知识2个维度得分低于干预前(P＜0.05),其他维度比较差异无统计学意义(P＞0.05);强化-维持干预后CSMS总分及各维度得分优于干预前(P＜0.05);强化-维持干预后再入院率明显低于干预前(P＜0.05).结论 强化-维持健康教育能有效提高并维持社区冠心病患者疾病相关知识水平,从而提升其自我管理能力,降低其再入院率.
语种：
中文
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治疗性沟通系统对肝硬化患者家属疾病不确定感的影响
作者：
李萍;李燕玲;王紫蔺;王旋;张屹冰
期刊：
临床消化病杂志 ,2019年31(1):37-40 ISSN：1005-541X
作者机构：
华中科技大学协和医院消化内科,湖北 武汉,430022;武汉轻工大学医学技术与护理学院,湖北 武汉,430023;[王紫蔺; 李燕玲; 李萍] 华中科技大学同济医学院附属协和医院;[张屹冰; 王旋] 武汉轻工大学
关键词：
肝硬化;治疗性沟通系统;家属;疾病不确定感
摘要：
[目的]分析60例肝硬化患者家属的疾病不确定感水平,探索治疗性沟通系统对肝硬化患者家属疾病不确定感的干预效果。[方法]采用自行设计的问卷和疾病不确定感家属量表对60例肝硬化患者家属进行调查;根据患者住院号尾数的奇数、偶数分为对照组、观察组各30例。对照组以常规健康教育方式,观察组以治疗性沟通系统方式。[结果]肝硬化患者家属疾病不确定感总分为78.00±10.69。观察组疾病不确定感、不明确性维度、缺乏澄清维度得分均低于对照组,2组比较差异有统计学意义;观察组干预后疾病不确定感及各维度得分均降低,2组比较差异有统计学意义。[结论]肝硬化患者家属疾病不确定感处于中等水平,治疗性沟通系统有助于降低其疾病不确定感水平。
语种：
中文
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瑞舒伐他汀预处理对缺血再灌注大鼠大脑中动脉血管平滑肌细胞炎症因子的影响及其机制
作者：
刘亚军;王旋;胡海;桂艳
期刊：
解剖学报 ,2019年50(6):713-717 ISSN：0529-1356
通讯作者：
Wang, X.
作者机构：
[王旋; 胡海; 桂艳; 刘亚军] School of Medical Technology and Nursing Science, Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Wang, X.] S;School of Medical Technology and Nursing Science, China
关键词：
瑞舒伐他汀;血管平滑肌细胞;白细胞介素;肿瘤坏死因子α;核因子-κB;缺血再灌注;实时定量聚合酶链反应;免疫印迹法;大鼠
摘要：
目的 探讨瑞舒伐他汀预处理对大鼠局灶性脑缺血再灌注后大脑中动脉血管平滑肌细胞(VSMCs)中炎性细胞因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)表达的影响以及可能的机制.方法 36只健康成年SD大鼠,雌雄不限,随机分为假手术组,局灶性脑缺血再灌注组,瑞舒伐他汀预处理组,每组12只.大脑中动脉缺血2h再灌注24 h后,Real-time PCR及Western blotting法检测大鼠大脑中动脉VSMCs中IL-1β、IL-6和TNF-α以及核因子κB(NF-κB) mRNA和蛋白的表达.结果 再灌注24 h后,模型组VSMCs IL-1β、IL-6以及TNF-αmRNA和蛋白的表达明显增加,而给予瑞舒伐他汀预处理后,可明显抑制大脑中动脉VSMCs中IL-1β、IL-6和TNF-αmRNA和蛋白的高表达,同时也可发现,VSMCs中NF-κB mRNA和蛋白的表达也明显减少.结论 瑞舒伐他汀预处理可有效抑制大脑中动脉VSMCs中IL-1β、IL-6和TNF-α mRNA和蛋白的表达,从而减轻缺血再灌注后炎症反应对脑组织的进一步损伤作用.瑞舒伐他汀预处理对IL-1β、IL-6及TNF-α的作用可能与其减少VSMCs中NF-κB的表达有关.
语种：
中文
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Urinary concentrations of environmental metals and associating factors in pregnant women.
作者：
Wang, Xin;Qi, Ling;Peng, Yang;Xia, Wei;Xu, Shunqing;...
期刊：
Environmental Science and Pollution Research ,2019年26(13):13464-13475 ISSN：0944-1344
通讯作者：
Zhang, Hongling;Li, Yuanyuan
作者机构：
[Zhang, Hongling; Wang, Xin; Qi, Ling] Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430023, Hubei, Peoples R China.;[Xia, Wei; Li, Yuanyuan; Wang, Xin; Xu, Shunqing; Peng, Yang] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Key Lab Environm & Hlth,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Li, Yuanyuan; Wang, Xin; Xu, Shunqing; Peng, Yang] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Minist Environm Protect, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Li, Yuanyuan; Wang, Xin; Xu, Shunqing; Peng, Yang] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, State Key Lab Environm Hlth Incubat, Wuhan 430030, Hubei, Peoples R China.
通讯机构：
[Zhang, Hongling] W;[Li, Yuanyuan] H;Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430023, Hubei, Peoples R China.;Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Key Lab Environm & Hlth,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Minist Environm Protect, Wuhan 430030, Hubei, Peoples R China.
关键词：
Associating factors;Pregnant women;Urinary metal concentrations
摘要：
Pregnant women, a vulnerable population, can be exposed to a variety of environmental metals that may adversely affect their health at elevated concentrations. Moreover, environmental exposure and risk disparities are associated with several factors such as sociodemographic characteristics, pregnancy complications, and nutritional supplementation indicators. Our aim was to investigate whether and how the urinary metal concentrations vary according to these factors in a large pregnancy and birth cohort. Urinary levels of seven metals including manganese, zinc, selenium, arsenic, cadmium, thallium, and lead were assessed in 7359 participants across Wuhan City in China. In all, 7359 urine samples from the third trimester were analyzed and 15 sociodemographic characteristics, pregnancy complications, and nutritional supplementation indicators were assessed: maternal age, household income, multivitamin supplements during pregnancy, etc. Concentrations of Zn, As, and Cd were detected in all urine samples; Mn, Se, Tl, and Pb were detected in 95.3%, 99.8%, 99.8%, and 99.9% urine samples, respectively. The geometric mean (GM) of creatinine-adjusted urinary Mn, Zn, Se, As, Cd, Tl, and Pb concentrations were 1.42, 505.44, 18.24, 30.49, 0.64, 0.55, and 3.69 mug/g. Factors that associated with environmental metals concentration were as follows: (1) Urinary Zn and Cd concentrations increased with maternal age. (2) Pregnant women with higher education had lower Cd concentrations. (3) Pregnant women with folic acid supplementation had lower Mn and with multivitamin supplementation had higher Se and lower Tl. Our results demonstrated that the maternal age, education level, folic acid supplementation, and multivitamin supplementation were significantly associated with environmental metal concentrations. Furthermore, studies are recommended to explore the influence of diet on biological metals concentrations in more detail.
语种：
英文
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病人配偶创伤后成长及其影响因素的研究
作者：
李应;王旋
期刊：
全科护理 ,2018年(26):3232-3234 ISSN：1674-4748
作者机构：
武汉轻工大学医学技术与护理学院
关键词：
创伤后成长;配偶;影响因素
摘要：
介绍创伤后成长概念内涵及病人配偶创伤后成长的测量、现状、影响因素,指出在创伤后成长领域的研究中还存在许多未解决的问题,目前大多数研究存在某些不足和局限性,但随着未来对创伤后成长深入研究,将会对严重的生活压力源所导致的增长带来更全面和更有用的理解。
语种：
中文
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Research and Implementation of Network Teaching Platform Based on Web
作者：
Wang, Xuan* ;Zhang, Hongling;Cheng, Qingzhou;Chen, Jing;Qiu, Nan
期刊：
PROCEEDINGS OF THE 6TH INTERNATIONAL CONFERENCE ON ELECTRONIC, MECHANICAL, INFORMATION AND MANAGEMENT SOCIETY (EMIM) ,2016年40:1041-1045 ISSN：2352-538X
通讯作者：
Wang, Xuan
作者机构：
[Zhang, Hongling; Cheng, Qingzhou; Wang, Xuan; Chen, Jing] Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan, Peoples R China.;[Qiu, Nan] Wuhan Univ Lib, Wuhan, Peoples R China.
通讯机构：
[Wang, Xuan] W;Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan, Peoples R China.
会议名称：
6th International Conference on Electronic, Mechanical, Information and Management Society (EMIM)
会议时间：
APR 01-03, 2016
会议地点：
Shenyang, PEOPLES R CHINA
会议主办单位：
[Wang, Xuan;Zhang, Hongling;Cheng, Qingzhou;Chen, Jing] Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan, Peoples R China.^[Qiu, Nan] Wuhan Univ Lib, Wuhan, Peoples R China.
会议论文集名称：
ACSR-Advances in Comptuer Science Research
关键词：
Online teaching;Support platform;Distance education;Web technology
摘要：
Nowadays, an effective teaching platform is an important factor in the distance education. In the paper, the structure and functional modules of the teaching platform system based on web technology is studied comprehensively, and these functional modules of the teaching platform is introduced in detail. And the implementation process of the main technical and design principles is generated in the paper. The platform has some function of management and teaching in the education based on multi-layer structure.
语种：
英文
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护理临床课程教学评价体系现状及对策
作者：
王旋;陈靖;张红菱
期刊：
消费导刊 ,2016年(4):374,376 ISSN：1672-5719
作者机构：
武汉轻工大学医学技术与护理学院;[王旋; 陈靖; 张红菱] 武汉轻工大学
关键词：
教学评价;教学改革;护理临床
摘要：
本文根据对护理临床课程的教学特点,对其教学评价体系进行分析,提出了包括诊断学评价、形成性评价、终结性评价等多元化的教学评价体系,对提高护理临床课程教学质量和深化护理教育改革具有积极的意义.
语种：
中文
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国际标准化护理语言在护理学科发展中的意义
作者：
王旋;程清洲;陈靖
期刊：
课程教育研究 ,2016年(10):241 ISSN：2095-3089
作者机构：
武汉轻工大学医学技术与护理学院 湖北 武汉 430023;[王旋; 陈靖; 程清洲] 武汉轻工大学
关键词：
国际标准化;护理语言;护理学科
摘要：
护理成为一级学科,必须形成国际标准化护理语言系统,才能促进沟通与协作,推动护理学科信息化建设、护理改革和护理科研的发展。
语种：
中文
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护理本科专业创新式实践教学探讨
作者：
王旋;张红菱;程清洲;卢玮;杨丽萍
期刊：
现代职业教育 ,2016年(1):40-41 ISSN：2096-0603
作者机构：
武汉轻工大学医学技术与护理学院,湖北武汉,430000;[王旋; 杨丽萍; 程清洲; 卢玮; 张红菱] 武汉轻工大学
关键词：
“任务驱动”教学法;“病例导入式”教学法;创新实践教学
摘要：
随着我国经济的持续较快发展,人们的健康需求和卫生消费也加速增长。普通高校的护理本科专业人才是按照社会需求来指定切合实际的培养目标和模式。将"任务驱动"教学法和"病例导入式教学法"应用于护理本科专业实践教学中,构建"以学生为中心"的实践教学新模式。
语种：
中文
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Beneficial effect of farnesoid X receptor activation on metabolism in a diabetic rat model
作者：
Zhang, Hong-Mei;Wang, Xuan;Wu, Zhao-Hong;Liu, Hui-Ling;Chen, Wei;...
期刊：
MOLECULAR MEDICINE REPORTS ,2016年13(3):2135-2142 ISSN：1791-2997
通讯作者：
Zeng, Tian-Shu
作者机构：
[Chen, Wei; Zhang, Hong-Mei; Zhang, Zhong-Zhi; Chen, Dan; Liu, Hui-Ling; Wu, Zhao-Hong] Hubei Integrated Tradit Chinese & Western Med Hos, Dept Endocrinol, Wuhan 430015, Hubei, Peoples R China.;[Wang, Xuan] Wuhan Polytech Univ, Dept Clin Teaching & Res, Coll Hlth Sci & Nursing, Wuhan 430015, Hubei, Peoples R China.;[Zeng, Tian-Shu] Huazhong Univ Sci & Technol, Union Hosp, Dept Endocrinol, Tongji Med Coll, 1277 Jiefang Rd, Wuhan 430030, Hubei, Peoples R China.
通讯机构：
[Zeng, Tian-Shu] H;Huazhong Univ Sci & Technol, Union Hosp, Dept Endocrinol, Tongji Med Coll, 1277 Jiefang Rd, Wuhan 430030, Hubei, Peoples R China.
关键词：
chenodeoxycholic acid;farnesoid X receptor;glucose;glucose 6 phosphatase;heterodimer;insulin;peroxisome proliferator activated receptor gamma;peroxisome proliferator activated receptor gamma coactivator 1;phosphoenolpyruvate carboxykinase (GTP);streptozocin;triacylglycerol;unclassified drug;cell receptor;chenodeoxycholic acid;farnesoid X-activated receptor;glucose 6 phosphatase;messenger RNA;peroxisome-proliferator-activated receptor-gamma coactivator-1;phosphoenolpyruvate carboxykinase (ATP);transcription factor;animal experiment;animal model;animal tissue;Article;body weight;controlled study;gene expression;gluconeogenesis;glucose blood level;insulin blood level;lipid diet;liver;male;non insulin dependent diabetes mellitus;nonhuman;protein expression;randomized controlled trial;rat;reverse transcription polymerase chain reaction;triacylglycerol blood level;Western blotting;animal;blood;disease model;drug effects;enzymology;experimental diabetes mellitus;gene expression regulation;genetics;metabolism;non insulin dependent diabetes mellitus;Wistar rat;Animals;Body Weight;Chenodeoxycholic Acid;Diabetes Mellitus, Experimental;Diabetes Mellitus, Type 2;Disease Models, Animal;Gene Expression Regulation;Gluconeogenesis;Glucose-6-Phosphatase;Liver;Male;Phosphoenolpyruvate Carboxykinase (ATP);Rats, Wistar;Receptors, Cytoplasmic and Nuclear;RNA, Messenger;Transcription Factors
摘要：
Farnesoid X receptor (FXR) is an important regulator of glucose and lipid homeostasis. However, the exact role of FXR in diabetes remains to be fully elucidated. The present study examined the effects of chenodeoxycholic acid (CDCA), an agonist of FXR, on metabolism profile in a rat model of type 2 diabetes mellitus (T2DM). Male Wistar rats (8-week-old; n=40) were randomized into the following four groups (n=10): Untreated control, CDCA-treated, T2DM, and CDCA-treated T2DM. To establish the T2DM model, the rats were fed a high-fat diet (HFD) for 4 weeks and received a single low-dose intraperitoneal injection of streptozotocin (30 mg/kg), followed by an additional 4 weeks of HFD feeding. CDCA was administrated (10 mg/kg/d) intraperitoneally for 10 days. Reverse transcription-quantitative polymerase chain reaction and western blotting assays were performed to determine the RNA and protein expression of FXR, phosphoenolpyruvate carboxykinase, G6Pase, proliferator-activated receptor-γ coactivator-1 and short heterodimer partner in rat liver tissue. The results revealed that FXR activation by CDCA did not reduce body weight, but it lowered the plasma levels of fasting glucose, insulin and triglycerides in the T2DM rats. CDCA administration reversed the downregulation of the mRNA and protein expression of FXR in the T2DM rat liver tissue samples. Furthermore, treatment with CDCA reduced the mRNA and protein expression levels of phosphoenolpyruvate carboxykinase, glucose 6-phosphatase and peroxisome proliferator-activated receptor-γ coactivator-1 in the liver tissue samples of the T2DM rats. By contrast, CDCA treatment increased the mRNA and protein expression levels of short heterodimer partner in the liver tissue samples of the T2DM rats. In conclusion, FXR agonist treatment induces beneficial effects on metabolism in the rat T2DM model. In conclusion, the present study indicated that the FXR agonist may be useful for the treatment of T2DM and hypertriglyceridemia.
语种：
英文
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Mycoplasmal lipoprotein p37 binds human protein HER2
作者：
Wu, Jun;Wu, Lijuan;Fang, Cheng;Nie, Rong;Wang, Jiamou;...
期刊：
Microbiological Research ,2016年192:253-259 ISSN：0944-5013
通讯作者：
Wang, Xuan;Liu, Wenbin
作者机构：
[Wu, Jun; Wu, Lijuan; Wang, Xuan; Liu, Wenbin; Fang, Cheng; Nie, Rong; Wang, Jiamou] Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430023, Peoples R China.;[Liu, Wenbin] Univ Florida, Coll Med, Gainesville, FL 32610 USA.
通讯机构：
[Wang, X; Liu, WB] W;Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430023, Peoples R China.
关键词：
Bins;Diseases;Pathology;Phosphorylation;Proteins;Cancer metastasis;Extracellular domains;HER2;Human disease;Human proteins;Mycoplasma;Signaling molecules;Signaling pathways;Lipoproteins;cancer;gene expression;lipid;protein;tumor;Mycoplasma;Mycoplasmatales;bacterial protein;epidermal growth factor receptor 2;lipoprotein;protein binding;animal;cell line;chemistry;gene expression;genetic transfection;genetics;human;immunohistochemistry;metabolism;mouse;mutation;Mycoplasma;phosphorylation;protein domain;Animals;Bacterial Proteins;Cell Line;Gene Expression;Humans;Immunohistochemistry;Lipoproteins;Mice;Mutation;Mycoplasma;Phosphorylation;Protein Binding;Protein Interaction Domains and Motifs;Receptor, ErbB-2;Transfection
摘要：
Mycoplasmas are a group of microbes that can cause human diseases. The mycoplasmal lipoprotein p37 promotes cancer metastasis, at least in part, by interacting with EGFR. In this study, we show that the p37 lipoprotein binds another member of the EGFR family, HER2, through the HER2 extracellular domain. The binding of p37-HER2 promotes phosphorylation of HER2 and activates the downstream signaling molecule Erk1/2. Because the HER2 signaling pathway contributes to breast tumor metastasis, our results imply that the mycoplasmal lipoprotein p37 may also be involved in breast cancer metastasis. This study contributes to our understanding of mycoplasmal lipoprotein p37 function and its potential involvement in tumorigenesis. © 2016
语种：
英文
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