Molecular Characterization of Cephalosporin-Resistant Salmonella Enteritidis ST11 Isolates Carrying blaCTX-M from Children with Diarrhea
作者：
Li, Congcong;Zhang, Zengfeng;Xu, Xuebin;He, Shoukui;Zhao, Xiaodong;...
期刊：
FOODBORNE PATHOGENS AND DISEASE ,2021年18(10):702-711 ISSN：1535-3141
通讯作者：
Shi, Xianming
作者机构：
[Li, Congcong; Zhao, Xiaodong; Zhang, Zengfeng; Zhou, Xiujuan; Shi, Xianming; He, Shoukui; Cui, Yan; Shi, Chunlei] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, Peoples R China.;[Xu, Xuebin] Shanghai Municipal Ctr Dis Control & Prevent, Dept Microbiol, Shanghai, Peoples R China.;[Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA.;[Zhou, Min] Wuhan Polytech Univ, Sch Food Sci & Engn, Dept Food Qual & Safety, Wuhan, Peoples R China.
通讯机构：
[Shi, Xianming] S;Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, Peoples R China.
关键词：
Salmonella Enteritidis;blaCTX-M-55;cephalosporin resistance;conjugative plasmid
摘要：
Salmonella Enteritidis is an important foodborne pathogen with high prevalence of resistance to cephalosporins, imposing a serious threat to public health. Therefore, a total of 162 Salmonella Enteritidis isolates collected from child patients in China from 2007 to 2017 were characterized for their resistance to cephalosporins and investigated the transmission characteristics of cephalosporin resistance gene. We found that 15 (9.26%) isolates were all resistant to cefalotin (minimum inhibitory concentration [MIC] ≥512 μg/mL), ceftazidime (MIC 16-128 μg/mL), ceftriaxone (MIC 64 to ≥512 μg/mL), ceftiofur (MIC 64-256 μg/mL), and cefotaxime (MIC 64 to ≥512 μg/mL) with the possession of cephalosporin resistance genes blaCTX-M-55 (n = 13), blaCTX-M-101 (n = 1), and blaCTX-M-153 (n = 1). Molecular typing further revealed that these 15 isolates belonged to sequence type ST11 and shared close pulsed-field gel electrophoresis patterns, suggesting the possibility of clonal spread in Salmonella Enteritidis interspecies. Furthermore, conjugation experiments were successfully performed in 13 of 15 isolates, and blaCTX-M-55 was present on conjugative plasmids with sizes ranging from 54.7 to 173.4 kb. Compared with recipient Escherichia coli C600, transconjugants conferred elevated MICs for cephalosporins ranging from 2- to 2048-fold. The genetic structure surrounding of blaCTX-M-55 gene in transconjugants were ΔISEcp1-blaCTX-M-55-orf477 (n = 8) and ISEcp1-blaCTX-M-55-orf477 (n = 3), respectively. Taken together, blaCTX-M on the plasmids might contribute to cephalosporin resistance in Salmonella Enteritidis, and conjugative transfer of blaCTX-M-55 might facilitate the spread of cephalosporin resistance in Salmonella Enteritidis. Hence, effective mitigation measurements are needed to reduce the threat caused by cephalosporin-resistant Salmonella Enteritidis to public health. © Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.
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β-glucosidase from Hevea brasiliensis seeds: Purification, homology modeling, and insights into the substrate-binding model
作者：
Xu, Jiaqian;Liu, Shisheng* ;Liu, Gang* ;Liu, Yu;He, Xu
期刊：
Journal of Food Biochemistry ,2020年44(6):e13206- ISSN：0145-8884
通讯作者：
Liu, Shisheng;Liu, Gang
作者机构：
[Liu, Shisheng; Xu, Jiaqian] Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Hainan, Peoples R China.;[Liu, Shisheng; Liu, Yu; Xu, Jiaqian; He, Xu] Minist Educ, Engn Res Ctr Utilizat Trop Polysaccharide Resourc, Haikou, Hainan, Peoples R China.;[Liu, Gang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Peoples R China.;[Liu, Gang] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430000, Peoples R China.
通讯机构：
[Liu, Shisheng] H;[Liu, Gang] W;Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Hainan, Peoples R China.;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430000, Peoples R China.
关键词：
Binding energy;Binding sites;Carbohydrates;Flavonoids;Flavor compounds;Free energy;Molecular dynamics;Physicochemical properties;Glucosidase;Homology modeling;Hydrolytic activities;Protein identification;Structural simulations;Substrate binding model;Substrate docking;Substrate-binding sites;Substrates
摘要：
The isolation of β-glucosidase from Hevea brasiliensis (Hbglu) seeds was investigated and a homology model was built on the MODELLER software to understand the structure feature. The quality of the model was evaluated on PROCHEK. The refined model was used for molecular docking on AutoDock 4.2 to determine the substrate- binding sites and potential substrates based on their calculated binding affinities. The substrate specificity of Hbglu was verified through the kinetic measurement of hydrolytic activities. Molecular dynamic simulations of cyanogenic β-glucosidase and ligand-bound complex showed that the free energy (ΔG) for the binding of p-nitrophenyl-β-D-glucopyranoside and daidzein-7-O-β-D-glucoside were −8.6 and −7.92kcal/mol, respectively. Thus, daidzein-7-O-β-D-glucoside is a potential substrate. Future studies on the physicochemical properties and catalytic mechanisms will provide information on the molecular biological properties of Hbglu. Practical applications: This study reported the 3D structural simulation of β-glucosidase from Hbglu. The docking condition between Hbglu and various different substrates were assessed on Autodock. The results can be used as reference in designing enzymes, and improving the utilization of β-glucosidases for the hydrolysis of flavor precursors from fruits, teas, and wines and for the production of flavonoid compounds and cyanogenic glycoside degradation. © 2020 Wiley Periodicals, Inc.
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Effects of different exogenous selenium on Se accumulation, nutrition quality, elements uptake, and antioxidant response in the hyperaccumulation plant Cardamine violifolia
作者：
Wu, Meiru;Cong, Xin;Li, Meng;Rao, Shen;Liu, Yuan;...
期刊：
Ecotoxicology and Environmental Safety ,2020年204:111045 ISSN：0147-6513
通讯作者：
Yu, Tian;Liu, Liping
作者机构：
[Yu, Tian; Wu, Meiru] Enshi Se Run Hlth Tech Dev Co Ltd, Enshi 445000, Peoples R China.;[Li, Meng] Beijing Technol & Business Univ, Beijing Key Lab Plant Resource Res & Dev, Beijing 100048, Peoples R China.;[Cheng, Shuiyuan; Yu, Tian; Wu, Meiru; Cong, Xin] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Rao, Shen; Xu, Feng] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Peoples R China.;[Liu, Yuan; Chen, Shaozhan; Liu, Liping] Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Trace Abil Technol Foo, Beijing 100013, Peoples R China.
通讯机构：
[Yu, Tian] E;[Liu, Liping] B;Enshi Se Run Hlth Tech Dev Co Ltd, Enshi 445000, Peoples R China.;Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Trace Abil Technol Foo, Beijing 100013, Peoples R China.
关键词：
Antioxidant response;Cardamine violifolia;Elements uptake;Exogenous selenium;Nutrition quality;Se accumulation
摘要：
Cardamine violifolia (Brassicaceae) is a novel selenium(Se) hyperaccumulation plant with rich nutrients, and serves as a good source of special vegetables in Enshi, China. The present study aimed to investigate the effects of the application of selenate, selenite, and Se yeast (50–800 mg/L) on the growth, Se accumulation, nutrient uptake, and antioxidant response of C. violifolia. The results showed that the Se accumulation efficiency was selenate > selenite > Se yeast, the maximum Se concentration could achieve over 7000 mg/kg, and about 90% was organic Se. The major Se speciation found was mainly SeCys2 and the proportion of various Se species were affected by the Se forms and concentrations. Besides, the plant growth, nutrition quality indexes, element uptakes, and antioxidant responses indicated that 200 mg/L selenate was optimum for C. violifolia to accumulate Se without much impacts, while to obtain more proportion of organic Se, 200 mg/L selenite might be a better choice. © 2020 Elsevier Inc.
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Prevalence of aflatoxin M1 in raw milk and three types of liquid milk products in central-south China
作者：
Xiong, Jianglin;Peng, Lijuan;Zhou, Hualin;Lin, Bo;Yan, Pingyu;...
期刊：
Food Control ,2020年108:106840 ISSN：0956-7135
通讯作者：
Wu, Lingying
作者机构：
[Liu, Yulan; Wu, Lingying; Xiong, Jianglin; Qiu, Yinsheng; Yan, Pingyu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China.;[Lin, Bo] Guangxi Univ, Coll Anim Sci, Nanning 530004, Peoples R China.;[Wu, Wenxuan] Guizhou Univ, Coll Anim Sci, Guiyang 550025, Guizhou, Peoples R China.
通讯机构：
[Wu, Lingying] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Aflatoxin M1;China;Milk products;Raw milk
摘要：
The objective of this study was to survey the prevalence of aflatoxin M1 (AFM1) in raw, pasteurized, extended shelf life (ESL) and UHT milk from central-south China. A total of 734 milk samples, comprising raw milk (133 samples), pasteurized milk (410), ESL milk (93) and UHT milk (98), were obtained between Nov. 01, 2018 and Mar. 31, 2019. The AFM1 concentrations in milk samples were detected by ELISA and confirmed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Five hundred and ninety samples (80.4%) were positive for AFM1; the mean value was 23.7 ng/L, and the range was 5.1–104.4 ng/L. The percentages of AFM1 positive raw, pasteurized, ESL and UHT milk samples were 75.2, 82.2, 81.7 and 78.6%, respectively. The AFM1 concentration in raw milk was significantly lower (p < 0.05) than that in pasteurized and ESL milk, and the AFM1 level in UHT milk tended to be lower (p < 0.10) than that in pasteurized and ESL milk. The AFM1 levels exceeded the EU legal limit of 50 ng/L in 0, 11.5, 18.3 and 3.1% of raw, pasteurized, ESL and UHT milk samples, respectively. All milk samples tested had levels far below China's legal limit of 500 ng/L. Therefore, the AFM1 content in raw milk and milk products in central-south China does not pose a public health threat, but close attention should nonetheless be paid to milk AFM1, given the high positive rate of 80.4%. © 2019
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Dispersive micro-solid phase extraction with fibrous TiO2@g-c3n4 nanocomposites coupled with icp-ms for the determination of cobalt and nickel in environmental and biological samples
作者：
Chen, Shizhong* ;Liu, Yuxiu;Yan, Juntao;Wang, Chunlei;Lu, Dengbo
期刊：
ATOMIC SPECTROSCOPY ,2020年41(4):169-174 ISSN：0195-5373
通讯作者：
Chen, Shizhong
作者机构：
[Chen, Shizhong; Lu, Dengbo] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Chunlei; Yan, Juntao; Liu, Yuxiu] Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Chen, Shizhong] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
摘要：
In this work, fibrous TiO2@g-C3N4 nanocomposites (FTGCNCs) were used for dispersive micro-solid phase extraction of Co and Ni prior to their determination by inductively coupled plasma mass spectrometry. As a new adsorbent, the surface on FTGCNCs have rich functional groups such as –NH2, –NH– and =N–, which can selectively adsorb target ions. The adsorption behaviors of Co and Ni on FTGCNCs were investigated. The results indicated that the analytes were quantitatively retained on FTGCNCs in the pH range of 5.0-8.0 and eluted completely with 1.0 mL of 0.5 mol L-1 HNO3 solution. The main factors influencing the preconcentration and determination of the analytes were investigated in detail, including the pH of the sample solution, dosage of adsorbent, extraction/elution time, elution parameters and sample volumes. Under the optimum conditions, the detection limits of this method were 0.12 pg mL-1 and 1.34 pg mL-1 for Co and Ni, respectively. The relative standard deviations were 3.9% for Co and 4.8% for Ni (n=9, c=1.0 ng mL-1). This method was used for the determination of trace/ultra-trace Co and Ni in environmental water samples with satisfactory results. In order to verify this method, three certified reference materials of stream sediment, human hair and mussel were analyzed using the proposed method, and the determined results were in agreement with the certified values. © 2020, Atomic Spectroscopy Press Limited. All rights reserved.
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Natural Occurrence of Deoxynivalenol and Its Acetylated Derivatives in Chinese Maize and Wheat Collected in 2017
作者：
Yan, Pianpian;Liu, Zhezhe;Liu, Shiqiao;Yao, Liyun;Liu, Yan;...
期刊：
TOXINS ,2020年12(3):200- ISSN：2072-6651
通讯作者：
Gong, Zhiyong
作者机构：
[Liu, Yan; Yan, Pianpian; Liu, Shiqiao; Gong, Zhiyong; Yao, Liyun; Liu, Zhezhe] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;[Wu, Yongning] Minist Hlth, Key Lab Food Safety Risk Assessment, China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China.
通讯机构：
[Gong, Zhiyong] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.
关键词：
deoxynivalenol;3-acetyl-deoxynivalenol;15-acetyl-deoxynivalenol;co-occurrence mycotoxins;wheat and maize;UHPLC-MS;MS
摘要：
Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. In this study, 579 wheat samples and 606 maize samples intended for consumption were collected from China in 2017 and analyzed to determine the co-occurrence of type-B trichothecenes (DON, 3-ADON, and 15-ADON). All the wheat samples tested positive for DON, while 99.83% of the maize samples were DON-positive with mean DON concentrations of 165.87 and 175.30 µg/kg, respectively. Per the Chinese standard limits for DON, 3.63% of wheat and 2.97% of the maize samples were above the maximum limit of 1000 µg/kg. The DON derivatives (3-ADON and 15-ADON) were less frequently found and were present at lower levels than DON in wheat. 3-ADON and 15-ADON had incidences of 13.53% and 76.40%, respectively, in maize. By analyzing the distribution ratio of DON and its derivatives in wheat and maize, DON (95.51%) was the predominant toxin detected in wheat samples, followed by 3.97% for the combination of DON + 3-ADON, while DON + 3-ADON + 15-ADON and DON + 15-ADON were only found in 0.17% and 0.35% of wheat samples, respectively. Additionally, a large amount of the maize samples were contaminated with DON + 15-ADON (64.19%) and DON (22.11%). The samples with a combination of DON + 3-ADON and DON + 3-ADON + 15-ADON accounted for 1.32% and 12.21%, respectively. Only one maize sample did not contain all three mycotoxins. Our study shows the necessity of raising awareness of the co-occurrence of mycotoxin contamination in grains from China to protect consumers from the risk of exposure to DON and its derivatives. © 2020 by the authors.
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Optimizing Rice Near-Infrared Models Using Fractional Order Savitzky–Golay Derivation (FOSGD) Combined with Competitive Adaptive Reweighted Sampling (CARS)
作者：
Xia, Zhenzhen;Yang, Jie;Wang, Jing;Wang, Shengpeng;Liu, Yan*
期刊：
Applied Spectroscopy ,2020年74(4):417-426 ISSN：0003-7028
通讯作者：
Liu, Yan
作者机构：
[Yang, Jie; Xia, Zhenzhen; Wang, Jing] Minist Agr, Inst Agr Qual Stand & Testing Technol Res, Hubei Acad Agr Sci, Lab Qual & Safety Risk Assessment Agroprod Wuhan, Wuhan, Peoples R China.;[Wang, Shengpeng] Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan, Peoples R China.;[Liu, Yan] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Peoples R China.;[Liu, Yan] Wuhan Polytech Univ, Xue Fu South Rd 68, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Xue Fu South Rd 68, Wuhan 430023, Peoples R China.
关键词：
NIR;PLS;Rice quality;competitive adaptive reweighted sampling;fractional order Savitzky-Golay derivation;near-infrared spectroscopy;partial least squares
摘要：
Developing a rapid and stable method for analyzing the quality parameters of rice is important. Near-infrared (NIR) spectroscopy combined with chemometric techniques have been used to predict the critical contents of rice and shown its accuracy and stability. To further improve the predictive ability, we combine the derivative method of fractional order Savitzky–Golay derivation (FOSGD) with the wavelength selection method of competitive adaptive reweighted sampling (CARS). Compared with the traditional integer order Savitzky–Golay derivation (IOSGD), the FOSGD could improve the resolution ratio of the raw spectra more effectively. The wavelength selection method, CARS, could further extract the informative variables from the processed spectra. Four key contents of rice samples, including moisture, amylose, chalkiness degree, and gel consistency, were utilized to validate this method. The prediction results indicated that partial least squares (PLS) models optimized with FOSGD-CARS own higher accuracy and stability with smaller the root mean squared error of cross validations (RMSECVs) and root mean squared error of predictions (RMSEPs). The proposed method is convenient and provides a practical alternative for rice analysis. © The Author(s) 2020.
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The neurotoxicity of Nε-(carboxymethyl)lysine in food processing by a study based on animal and organotypic cell culture
作者：
Wu, Yang;Li, Yan;Zheng, Liangqing;Wang, Pengcheng;Liu, Yan;...
期刊：
Ecotoxicology and Environmental Safety ,2020年190:110077 ISSN：0147-6513
通讯作者：
Wu, Yongning;Gong, ZhiYong
作者机构：
[Liu, Yan; Wu, Yang; Zheng, Liangqing; Gong, ZhiYong; Wang, Pengcheng] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Li, Yan] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Wu, Yongning] Minist Hlth, China Natl Ctr Food Safety Risk Assessment, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China.;[Gong, ZhiYong] Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Wu, Yongning] M;[Gong, ZhiYong] W;Minist Hlth, China Natl Ctr Food Safety Risk Assessment, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China.;Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
3D microvascular model;Nepsilon-(Carboxymethyl) lysine;Oxidative stress;p38MAPK-AP-1 signal pathway
摘要：
Nepsilon-(carboxymethyl)lysine (CML) is a potentially noxious compound that is causing widespread concern due to its use in various food products. In this study, we investigated CML neurotoxicity via an in vivo experiment with mice, and an in vitro experiment using a 3D microvascular network model (with human brain vascular endothelial cell and human astrocyte) that simulated the blood-brain barrier. We found that CML could induce cell survival status variations, and histopathological changes to the brain. In addition, CML increased levels of oxidative stress, prompted the protein expression of the receptor for advanced glycation end-products (RAGE). CML up-regulated both the gene expression of RAGE, the activating protein-1 (AP-1), the inflammatory cytokines Interleukin-6 (IL-6), vascular cell adhesion molecule1 (VCAM-1), monocyte chemotactic protein1 (MCP-1). We, therefore, postulated that CML has the potential to deleteriously affect the nervous system through oxidative stress and that activation of the p38 MAPK-AP-1 signaling pathway might be implicated in this pathological process.
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英文
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Study of the neurotoxicity of indoor airborne nanoparticles based on a 3D human blood-brain barrier chip
作者：
Li, Yan;Liu, Yan;Hu, Chuanlin;Chang, Qing;Deng, Qihong;...
期刊：
Environment International ,2020年143:105598 ISSN：0160-4120
通讯作者：
Yang, Xu;Wu, Yang
作者机构：
[Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Chinese Minist Educ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Hu, Chuanlin] Wuhan Univ Technol, State Key Lab Silicate Mat Architectures, Wuhan 430070, Peoples R China.;[Yang, Xu; Deng, Qihong] Cent South Univ, XiangYa Sch Publ Hlth, Changsha 410083, Peoples R China.;[Chang, Qing] Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Kowloon, Clear Water Bay, Hong Kong, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Sch Life Sci, Lab Environm Biomed, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Xu] C;[Wu, Yang] W;Cent China Normal Univ, Sch Life Sci, 152 Luo Yu Rd, Wuhan 430079, Peoples R China.;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Peoples R China.
关键词：
Indoor nanoscale particulate matter;3D microfluidic chip;blood-brain barrier;Oxidative stress;Nrf2/Keap1 signal pathway
摘要：
Background: There is growing public awareness regarding the health effects of indoor nanoscale particulate matter (INPM) since people spend the majority of their time indoors. INPM could have a direct entry route into the brain via the axons of the olfactory nerve and migrating across the blood-brain barrier (BBB). Using animals to explore this possibility is not a reliable method to fully demonstrate human physiological responses. We, therefore, set out to develop a human 3D functional blood-brain barrier model to examine the potential effects of INPM on the cerebral nervous system. Methods: Human astrocytes were co-cultured and human umbilical vein endothelial cells in 3D within a microfluidic chip to simulate the micro-complex physiological structure of the human BBB. This 3D human organotypic model has then been made to investigate any INPM-induced BBB dysfunction linked to potential cellular responses. Results: A 3D human functional blood-brain barrier was constructed in this study. We observed the translocation of INPM across the blood-brain barrier. The 3D human organotypic chip initially reflected damage to the nervous system with abnormal astrocyte proliferation and a decline in cell viability. We also looked at the behavior of oxidative stress-related biomarkers (ROS, GSH-Px, and MDA). INPM was implicated in aggravating inflammation via reactive oxygen species (ROS). The Keap1-Nrf2-ARE pathway is a key mechanism in cellular resistance to oxidative stress by mediating and activating a variety of antioxidant and detoxification enzymes. Following ROS accumulation, INPM induced abnormal expression of nuclear transcription factor Nrf2. This behavior disturbed the expression of, γ-glutamate synthase (γ-GCS) and heme oxygenase (HO-1), which further exacerbated the imbalance of the antioxidant system. Conclusions: This functional 3D human organotypic chip effectively mimics the physiological response of the human BBB. The chip provides a micro-complex structure to simulate the internal environment of the human blood-brain barrier, and partially simulates the physiological responses of the BBB to INPM exposure. Based on this model, INPM was shown to affect the blood-brain barrier biofunction by disrupting the Keap1-Nrf2-ARE pathways. © 2020 The Authors
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Determination of Antimony Speciation in Cow Milk Using Dispersive Micro-solid Phase Extraction Based on Fibrous TiO2@g-C(3)N(4)Nanocomposites and ICP-MS After Sample Pretreatment by Artificial Gastric Juice
作者：
Chen, Shizhong* ;Liu, Yuxiu;Wang, Chunlei;Yan, Juntao;Lu, Dengbo
期刊：
Food Analytical Methods ,2020年13(11):2102-2110 ISSN：1936-9751
通讯作者：
Chen, Shizhong
作者机构：
[Chen, Shizhong; Lu, Dengbo] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Chunlei; Yan, Juntao; Liu, Yuxiu] Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Chen, Shizhong] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Antimony speciation;Artificial gastric juice;Cow milk;Dispersive micro-solid phase extraction;Fibrous TiO2@g-C3N4 nanocomposites;Inductively coupled plasma mass spectrometry
摘要：
In this work, fibrous TiO2@g-C3N4 nanocomposites (FTGCNCs) were used as a new adsorbent of dispersive micro-solid phase extraction for speciation of antimony in cow milk, including Sb(III), Sb(V), residual, digestible, and total Sb. Samples were prepared with artificial gastric juice to achieve bioaccessible species of elements before ICP-MS determination. Besides, adsorption behaviors of Sb(III) and Sb(V) on FTGCNCs were investigated. The results showed that Sb(III) was quantitatively adsorbed on FTGCNCs in the pH range of 2.0–4.0, while Sb(V) remained in aqueous phase. Sb(III) adsorbed on FTGCNCs was eluted completely with 1.5molL−1 HNO3 solution. The assay of Sb(V) was based on subtracting Sb(III) from total Sb. Total Sb was extracted and determined after the reduction of Sb(V) into Sb(III). The main parameters affecting the separation and determination of the analytes were examined in detail. Under optimal conditions, the limit of detection was 0.37pgmL−1 for Sb(III) with relative standard deviation of 4.1% (c = 1.0ngmL−1, n = 9). This method was successfully used for the analysis of Sb speciation in cow milk. To validate this method, a certified reference material of milk powder was analyzed, and the determined values were in good agreement with the certified values. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
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Simultaneous and rapid determination of sesamin and sesamolin in sesame oils using excitation-emission matrix fluorescence coupled with self-weighted alternating trilinear decomposition
作者：
Liu, Yan* ;Wu, Qian;Xia, Zhenzhen;Wu, Yang;Li, Yan;...
期刊：
Journal of the Science of Food and Agriculture ,2020年100(12):4418-4424 ISSN：0022-5142
通讯作者：
Liu, Yan
作者机构：
[Liu, Yan; Wu, Yang; Gong, Zhiyong; Li, Yan; Wu, Qian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Liu, Yan; Wu, Yang; Gong, Zhiyong; Li, Yan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Peoples R China.;[Liu, Yan; Wu, Yang; Gong, Zhiyong; Li, Yan] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
1,3 dioxolane derivative;lignan;sesame seed oil;sesamin;sesamolin;chemistry;fluorescence;procedures;Sesamum;spectrofluorometry;Dioxoles;Fluorescence;Lignans;Sesame Oil;Sesamum;Spectrometry, Fluorescence
摘要：
BACKGROUND: Sesamin and sesamolin are two typical and important lignans isolated from sesame oil. Various studies have shown the bioactivity, physiological activity, and potential health benefits of the two components. In this study, a rapid method for the simultaneous determination of sesamin and sesamolin in sesame oils was proposed. The excitation-emission fluorescence spectra of the oils were obtained after a simple pretreatment, then self-weighted alternating trilinear decomposition was used to extract the quantitative information from the very overlapping spectra. RESULTS: It was found that reasonable quantification results could be obtained with the limits of detection for the two lignans. These limits were 0.05 mg/g and 0.24 mg/g, and the limits of quantitation were 0.14 mg/g and 0.74 mg/g, respectively. The average recoveries for sesamin and sesamolin were 99.05% and 94.97%. CONCLUSION: The results indicate that, with simple sample pretreatment, the application for combining excitation-emission fluorescence spectra and self-weighted alternating trilinear decomposition can be a useful and sensitive tool for the determination of lignans in sesame oil. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry
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英文
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Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip
作者：
Li, Yan;Hu, Chuanlin;Wang, Pengcheng;Liu, Yan;Wang, Luyang;...
期刊：
Journal of Nanobiotechnology ,2019年17(1):1-17 ISSN：1477-3155
通讯作者：
Wu, Yang;Mak, Michael
作者机构：
[Liu, Yan; Gong, Zhiyong; Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Wu, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Wang, Luyang] Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.;[Pi, Qingmeng] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA.
通讯机构：
[Wu, Yang] W;[Mak, Michael] Y;Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.
关键词：
Indoor particulate matter;Oxidative stress;Inflammation;Promote coagulation;3D human microvessel
摘要：
Background: A growing body of evidence shows that indoor concentrations of airborne particles are often higher than is typically encountered outdoors. Since exposure to indoor PM2.5 is thought to be associated with cardiovascular disease, the health impacts of indoor air pollution need to be explored. Based on animal models, ambient particulate matter has been proved to promote coagulation which is very likely involved in the pathogenic development of cardiovascular disease. However, animal models are insufficient to predict what will happen with any certainty in humans. For this reason, the precise pathogenic mechanisms behind the development of cardiovascular disease in humans have not yet been determined. Results: We generated a 3D functional human microvascular network in a microfluidic device. This model enables human vascular endothelial cells to form tissue-like microvessels that behave very similarly to human blood vessels. The perfusable microvasculature allows the delivery of particles introduced into these generated human-like microvessels to follow the fluid flow. This exposure path effectively simulates the dynamic movement of airborne nanoscale particles (ANPs) within human vessels. In this study, we first identified the existence of ANPs in indoor air pollution. We then showed that ANPs could activate endothelial cells via ROS induced inflammation, and further resulted in abnormal expression of the coagulation factors (TF, TM and t-PA) involved in coagulation cascades. In addition, we found that a protein could cover ANPs, and this biointeraction could interfere with heparan sulfate (HS). Human organotypic 3D microvessel models provide a bridge for how research outcomes can translate to humans. Conclusions: The 3D human microvessel model was used to determine the physiological responses of human vessels to ANP stimulation. Based on the obtained data, we concluded that ANPs not only disrupts normal coagulation functions, but also act directly on anticoagulant factors in human vessels. These experimental observations provide a potential biological explanation for the epidemiologically established link between ANPs and coagulation abnormality. This organ-on-chip model may provide a bridge from in vitro results to human responses. © 2019 The Author(s).
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英文
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Discriminating geographic origin of sesame oils and determining lignans by near-infrared spectroscopy combined with chemometric methods
作者：
Liu, Yan* ;Xia, Zhenzhen;Yao, Liyun;Wu, Yang;Li, Yan;...
期刊：
Journal of Food Composition and Analysis ,2019年84:103327 ISSN：0889-1575
通讯作者：
Liu, Yan
作者机构：
[Liu, Yan; Wu, Yang; Li, Yan; Yao, Liyun] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Hubei, Peoples R China.;[Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil Wuhan Polytec, Minist Educ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod Wuhan P, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Li, Hao; Zeng, Shan] Wuhan Polytech Univ, Coll Math & Comp Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Near-infrared spectroscopy;Sesame oil;Lignan;Linear discriminant analysis;Partial least squares
摘要：
Near-infrared spectroscopy (NIRS) combined with chemometric methods were used to discriminate the sesame oils from different Chinese provinces and determinate the lignans (sesamin and sesamolin) in these sesame oils. The geographic discriminant model was constructed by principal component analysis (PCA) combining with linear discriminant analysis (LDA), and the quantitative analysis models of lignans were built using partial least squares (PLS) regression. Multiplicative scatter correction (MSC) and competitive adaptive reweighted sampling (CARS) were adopted to optimize the regression models. It was found that the discriminant model could recognize the sesame oils from different Chinese provinces correctly, and the contents of sesamin and sesamolin calculated from the optimized models and high-performance liquid chromatography (HPLC) analysis are rather close. Reasonable results proved the feasibility of near-infrared spectroscopy (NIRS) combined with chemometric methods for geographic origin of sesame oils and quantitate analysis of lignans in sesame oils. © 2019 Elsevier Inc.
语种：
英文
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Atmospheric nanoparticles affect vascular function using a 3D human vascularized organotypic chip
作者：
Li, Yan;Wu, Yang;Liu, Yan;Deng, Qi-Hong;Mak, Michael* ;...
期刊：
Nanoscale ,2019年11(33):15537-15549 ISSN：2040-3364
通讯作者：
Mak, Michael;Yang, Xu
作者机构：
[Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Chinese Minist Educ, Wuhan 430023, Hubei, Peoples R China.;[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Deng, Qi-Hong] Cent S Univ, XiangYa Sch Publ Hlth, Changsha 410083, Hunan, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Lab Environm Biomed, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Mak, Michael] Y;[Yang, Xu] C;Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;Cent China Normal Univ, Lab Environm Biomed, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
Blood vessels;Cell adhesion;Cell engineering;Endothelial cells;Mammals;Nanoparticles;Nitric oxide;Proteins;Toxicity;Biological behavior;Cell adhesion molecules;Human blood vessels;Micro-fluidic devices;Nuclear factor kappaB;Pathological response;Physiological response;Vascular endothelial growth factor;Physiological models;calcium;immunoglobulin enhancer binding protein;intercellular adhesion molecule 1;nanoparticle;nitric oxide;protein corona;protein ZO1;vasculotropin A;animal;Bagg albino mouse;biological model;cell culture technique;chemistry;confocal microscopy;human;lab on a chip;male;metabolism;microvasculature;mouse;pathology;tissue engineering;umbilical vein endothelial cell;Animals;Calcium;Cell Culture Techniques;Human Umbilical Vein Endothelial Cells;Humans;Intercellular Adhesion Molecule-1;Lab-On-A-Chip Devices;Male;Mice;Mice, Inbred BALB C;Microscopy, Confocal;Microvessels;Models, Cardiovascular;Nanoparticles;NF-kappa B;Nitric Oxide;Protein Corona;Tissue Engineering;Vascular Endothelial Growth Factor A;Zonula Occludens-1 Protein
摘要：
Inhaled atmospheric nanoparticles (ANPs) can migrate into human blood vessels. However, the exact pathogenesis has not yet been well elucidated. In this study, a perfusable 3D human microvessel network was constructed in a microfluidic device. This functional 3D micro-tissue partly mimicked the physiological response of human vessels. Intravascular nanoparticles tend to adsorb proteins to form a protein corona. Based on this pathological response, vessel permeability and vasoconstriction resulting from ANP stimulation might be related to vascular inflammation. It mediated abnormal expression of nuclear factor-κB (NF-κB) and an influx of intracellular Ca2+ ([Ca2+]i). This biological behavior disturbed the normal expression of intercellular cell adhesion molecule 1 (ICAM-1) and vascular endothelial growth factor (VEGF). The imbalance of nitric oxide (NO) and endothelin-1 (ET-1) further resulted in endothelial cell contraction. All these bio-events induced the loss of tight junctions (ZO-1) which enhanced vessel permeability. Meanwhile, ANP induced-vascular toxicity was also found in mice. Our observations provide a plausible explanation for how the ANPs affect human vascular function. The vessel-on-chip provides a bridge between in vitro results and human responses. We aimed to use this human 3D functional microvascular model to mimic the physiological responses of human vessels. This model is suitable for the evaluation of vascular toxicity after the human vessel exposure to ANPs. © The Royal Society of Chemistry.
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英文
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Structures of Complexes of Gossypol with Ferrous Sulfate Based on High-Performance Liquid Chromatography Separation, Spectroscopic Analysis, and PM3 calculations
作者：
Zhang, Jian* ;Ran, Han;Xiong, Wei;Yang, Tianyi;Mei, Yunjun;...
期刊：
ChemistrySelect ,2019年4(19):5484-5488 ISSN：2365-6549
通讯作者：
Zhang, Jian;Song, Guangsen
作者机构：
[Liu, Ye; Cheng, Qunpeng; Zhang, Jian; Mei, Yunjun; Song, Guangsen; Li, Shenglan; Zhang, J; Song, GS; Yang, Tianyi; Ran, Han; Xiong, Wei] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.;[He, Dongping] Wuhan Polytech Univ, Sch Food Sci & Technol, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhang, J; Song, GS] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Iron-gossypol complex;Liquid chromatography;PM3 calculations;Spectroscopic analysis;Structure elucidation
摘要：
The complexes of gossypol with Fe2+ were prepared by using methanol as solvent in the presence of CO2 atmosphere. The results from FT-IR spectra analysis indicated that the aromatic aldehyde groups in gossypol molecules were entirely involved in coordination with Fe2+. UV-Vis absorption spectrum of the complexes in methanol solution displays blue-shift in comparison with that of gossypol. Five compounds from the complexes were isolated by high performance liquid chromatography. The structures of the complexes are consistent with PM3 calculations. Two compounds were formed by coordination of gossypol with Fe2+ at 1:2 molar ratio, other three compounds were formed by chelation of gossypol with Fe2+ at molar ratio by 1:1, 1:3, and 1:4 respectively.
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英文
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Rapid determination of phytosterols by NIRS and chemometric methods
作者：
Liu, Yan* ;Wang, Yixin;Xia, Zhenzhen;Wang, Yingjie;Wu, Yongning;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2019年211:336-341 ISSN：1386-1425
通讯作者：
Liu, Yan
作者机构：
[Liu, Yan; Wang, Yingjie; Wang, Yixin; Gong, Zhiyong; Wu, Yongning] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Hubei, Peoples R China.;[Liu, Yan; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yan; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Near-infrared spectroscopy;Partial least squares;Phytosterol;Vegetable oil;Wavelength selection
摘要：
Phytosterols have been extensively studied because it plays essential roles in the physiology of plants and can be used as nutritional supplement to promote human health. We use a rapid method by coupling near-infrared spectroscopy (NIRS) and chemometric techniques to quickly and efficiently determine three essential phytosterols (beta-sitosterol, campesterol and stigmasterol) in vegetable oils. Continuous wavelet transform (CWT) method was adopted to remove the baseline shift in the spectra. The quantitative analysis models were constructed by partial least squares (PLS) regression and randomization test (RT) method was used to further improve the models. The optimized models were used to calculate the phytosterol contents in prediction set in order to evaluate their predictability. We have found that the phytosterol contents obtained by the optimized models and Gas Chromatography/Mass Spectrometry (GC/MS) analysis are almost consistent. The root mean square error of prediction (RMSEP) and ratio of prediction to deviation (RPD) for the three phytosterols are 525.7590, 212.2245, 65.1611 and 4.0060, 4.7195 and 3.5441, respectively. The results have proved the feasibility of the proposed method for rapid and non-destructive analysis of phytosterols in edible oils.
语种：
英文
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Multi-spectrometer calibration transfer based on independent component analysis
作者：
Liu, Yan* ;Xu, Hao;Xia, Zhenzhen;Gong, Zhiyong
期刊：
ANALYST ,2018年143(5):1274-1280 ISSN：0003-2654
通讯作者：
Liu, Yan
作者机构：
[Liu, Yan; Xu, Hao; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Hubei, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
摘要：
Calibration transfer is indispensable for practical applications of near infrared (NIR) spectroscopy due to the need for precise and consistent measurements across different spectrometers. In this work, a method for multi-spectrometer calibration transfer is described based on independent component analysis (ICA). A spectral matrix is first obtained by aligning the spectra measured on different spectrometers. Then, by using independent component analysis, the aligned spectral matrix is decomposed into the mixing matrix and the independent components of different spectrometers. These differing measurements between spectrometers can then be standardized by correcting the coefficients within the independent components. Two NIR datasets of corn and edible oil samples measured with three and four spectrometers, respectively, were used to test the reliability of this method. The results of both datasets reveal that spectra measurements across different spectrometers can be transferred simultaneously and that the partial least squares (PLS) models built with the measurements on one spectrometer can predict that the spectra can be transferred correctly on another.
语种：
英文
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重组米的研究进展
作者：
郭亚丽;程科;王辉;张朝富;李志方;...
期刊：
粮食与饲料工业 ,2018年(9):1-4 ISSN：1003-6202
作者机构：
国粮武汉科学研究设计院有限公司,湖北武汉,430079;武汉轻工大学食品科学与工程学院,湖北武汉,430023;[李志方; 谢健; 左家瑞; 张朝富; 程科; 杨会宾; 王辉; 郭亚丽] 国粮武汉科学研究设计院;[刘英] 武汉轻工大学
关键词：
重组米;原配料;生产工艺;食用品质;改良剂;应用前景
摘要：
重组米是以碎米、米粉或其他淀粉基质类原料为主要成分,经粉碎、调质后进入挤压机重新制粒、干燥而得。重组米与普通大米相比,在营养强化、成本控制等方面具有显著优势。国内外科研工作者对重组米的研究较为丰富,得到大量研究成果。主要从原配料、生产工艺、品质特性及改良剂等方面论述了近年来国内外重组米领域的相关研究,以期为重组米新产品的开发提供参考。
语种：
中文
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Chemical and rheological properties of polysaccharides from litchi pulp
作者：
Huang, Fei;Liu, Yang;Zhang, Ruifen;Dong, Lihong;Yi, Yang;...
期刊：
International Journal of Biological Macromolecules ,2018年112:968-975 ISSN：0141-8130
通讯作者：
Zhang, Mingwei
作者机构：
[Deng, Yuanyuan; Dong, Lihong; Wei, Zhencheng; Huang, Fei; Liu, Yang; Zhang, Ruifen; Zhang, Mingwei] Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Key Lab Funct Foods, Sericultural & Agrifood Res Inst,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Wang, Guangjin] Hubei Engn Univ, Coll Chem & Mat Sci, Xiaogan 432000, Peoples R China.;[Zhang, Mingwei] Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Guangdong, Peoples R China.
通讯机构：
[Zhang, Mingwei] G;Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Guangdong, Peoples R China.
关键词：
Litchi pulp;Polysaccharide;theological properties
摘要：
Litchi polysaccharide (IP) was extracted from litchi pulp. Its chemical composition, microstructure, zeta potential, flow and viscoelastic behavior were investigated. LP contained uronic acid (41.18%), neutral sugar (4223%), and protein (2.72%). The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose. Scanning electron microscopy (SEM) showed IP was porous network structure. LP concentration had no effect on its zeta potential value while salts reduced them. LP showed shear-thinning behavior during the tested shear rate range. The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations. The viscosity of LP increased under acidic conditions (pH 2-4), but was stable with heat treatment. The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2. (C) 2018 Published by Elsevier B.V.
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英文
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水热处理对大米粉及抗性淀粉性质影响
作者：
许诗尧;李芳;胡忠泽;刘刚;秦新光;...
期刊：
中国粮油学报 ,2017年32(6):1-7 ISSN：1003-0174
通讯作者：
Liu, Y.
作者机构：
[许诗尧; 李芳; 胡忠泽; 刘刚; Qing X.] Food Science College of Wuhan Polytechnic University, Wuhan, 430023, China;Hubei Rice Engineering and Technology Research Center, Wuhan, 430023, China;[刘英] Food Science College of Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Hubei Rice Engineering and Technology Research Center, Wuhan, 430023, China
通讯机构：
Food Science College of Wuhan Polytechnic University, Wuhan, China
关键词：
大米粉;抗性淀粉;水热处理
摘要：
以大米粉为原料,研究压热法、湿热法、韧化法处理对大米粉及其抗性淀粉成分微观结构、结晶学和热力学性质的影响。研究结果表明,压热处理后,大米粉及其抗性淀粉晶型由A型转化为特殊B型,抗性淀粉在20 = 12.9。、19.8。出现2个特征峰象征脂质淀粉复合物的形成;湿热处理后,大米粉形成有一定淀粉酶抗性的外壳结构,晶型转向A型,升温在115 ℃附近出现巨大的特征吸热峰;韧化处理后形成了粒度较大但内部疏松的淀粉团粒。表明压热处理后,大米粉及其抗性淀粉形成特殊晶体结构,晶体和热稳定性均提高;湿热处理对大米粉结晶学和热力学性质有一定影响,但对抗性淀粉影响不大;韧化处理对大米粉及抗性淀粉结晶和热力学性质影响不大。
语种：
中文
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