作者： Li, Yan;Hu, Chuanlin;Wang, Pengcheng;Liu, Yan;Wang, Luyang;...

期刊： Journal of Nanobiotechnology,2019年17(1):1-17 ISSN：1477-3155

通讯作者： Wu, Yang;Mak, Michael

作者机构： [Liu, Yan; Gong, Zhiyong; Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Wu, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Wang, Luyang] Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.;[Pi, Qingmeng] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA.

通讯机构： [Wu, Yang] W;[Mak, Michael] Y;Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.

关键词： Indoor particulate matter;Oxidative stress;Inflammation;Promote coagulation;3D human microvessel

摘要： Background: A growing body of evidence shows that indoor concentrations of airborne particles are often higher than is typically encountered outdoors. Since exposure to indoor PM2.5 is thought to be associated with cardiovascular disease, the health impacts of indoor air pollution need to be explored. Based on animal models, ambient particulate matter has been proved to promote coagulation which is very likely involved in the pathogenic development of cardiovascular disease. However, animal models are insufficient to predict what will happen with any certainty in humans. For this reason, the precise pathogenic mechanisms behind the development of cardiovascular disease in humans have not yet been determined. Results: We generated a 3D functional human microvascular network in a microfluidic device. This model enables human vascular endothelial cells to form tissue-like microvessels that behave very similarly to human blood vessels. The perfusable microvasculature allows the delivery of particles introduced into these generated human-like microvessels to follow the fluid flow. This exposure path effectively simulates the dynamic movement of airborne nanoscale particles (ANPs) within human vessels. In this study, we first identified the existence of ANPs in indoor air pollution. We then showed that ANPs could activate endothelial cells via ROS induced inflammation, and further resulted in abnormal expression of the coagulation factors (TF, TM and t-PA) involved in coagulation cascades. In addition, we found that a protein could cover ANPs, and this biointeraction could interfere with heparan sulfate (HS). Human organotypic 3D microvessel models provide a bridge for how research outcomes can translate to humans. Conclusions: The 3D human microvessel model was used to determine the physiological responses of human vessels to ANP stimulation. Based on the obtained data, we concluded that ANPs not only disrupts normal coagulation functions, but also act directly on anticoagulant factors in human vessels. These experimental observations provide a potential biological explanation for the epidemiologically established link between ANPs and coagulation abnormality. This organ-on-chip model may provide a bridge from in vitro results to human responses. © 2019 The Author(s).

语种： 英文

作者： Zhang, Jian*;Ran, Han;Xiong, Wei;Yang, Tianyi;Mei, Yunjun;...

期刊： ChemistrySelect,2019年4(19):5484-5488 ISSN：2365-6549

通讯作者： Zhang, Jian;Song, Guangsen

作者机构： [Liu, Ye; Cheng, Qunpeng; Zhang, Jian; Mei, Yunjun; Song, Guangsen; Li, Shenglan; Zhang, J; Song, GS; Yang, Tianyi; Ran, Han; Xiong, Wei] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.;[He, Dongping] Wuhan Polytech Univ, Sch Food Sci & Technol, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Zhang, J; Song, GS] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： Iron-gossypol complex;Liquid chromatography;PM3 calculations;Spectroscopic analysis;Structure elucidation

摘要： The complexes of gossypol with Fe2+ were prepared by using methanol as solvent in the presence of CO2 atmosphere. The results from FT-IR spectra analysis indicated that the aromatic aldehyde groups in gossypol molecules were entirely involved in coordination with Fe2+. UV-Vis absorption spectrum of the complexes in methanol solution displays blue-shift in comparison with that of gossypol. Five compounds from the complexes were isolated by high performance liquid chromatography. The structures of the complexes are consistent with PM3 calculations. Two compounds were formed by coordination of gossypol with Fe2+ at 1:2 molar ratio, other three compounds were formed by chelation of gossypol with Fe2+ at molar ratio by 1:1, 1:3, and 1:4 respectively.

语种： 英文

作者： Li, Yan;Wu, Yang;Liu, Yan;Deng, Qi-Hong;Mak, Michael*;...

期刊： Nanoscale,2019年11(33):15537-15549 ISSN：2040-3364

通讯作者： Mak, Michael;Yang, Xu

作者机构： [Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Chinese Minist Educ, Wuhan 430023, Hubei, Peoples R China.;[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Deng, Qi-Hong] Cent S Univ, XiangYa Sch Publ Hlth, Changsha 410083, Hunan, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Lab Environm Biomed, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.

通讯机构： [Mak, Michael] Y;[Yang, Xu] C;Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;Cent China Normal Univ, Lab Environm Biomed, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.

关键词： Blood vessels;Cell adhesion;Cell engineering;Endothelial cells;Mammals;Nanoparticles;Nitric oxide;Proteins;Toxicity;Biological behavior;Cell adhesion molecules;Human blood vessels;Micro-fluidic devices;Nuclear factor kappaB;Pathological response;Physiological response;Vascular endothelial growth factor;Physiological models;calcium;immunoglobulin enhancer binding protein;intercellular adhesion molecule 1;nanoparticle;nitric oxide;protein corona;protein ZO1;vasculotropin A;animal;Bagg albino mouse;biological model;cell culture technique;chemistry;confocal microscopy;human;lab on a chip;male;metabolism;microvasculature;mouse;pathology;tissue engineering;umbilical vein endothelial cell;Animals;Calcium;Cell Culture Techniques;Human Umbilical Vein Endothelial Cells;Humans;Intercellular Adhesion Molecule-1;Lab-On-A-Chip Devices;Male;Mice;Mice, Inbred BALB C;Microscopy, Confocal;Microvessels;Models, Cardiovascular;Nanoparticles;NF-kappa B;Nitric Oxide;Protein Corona;Tissue Engineering;Vascular Endothelial Growth Factor A;Zonula Occludens-1 Protein

摘要： Inhaled atmospheric nanoparticles (ANPs) can migrate into human blood vessels. However, the exact pathogenesis has not yet been well elucidated. In this study, a perfusable 3D human microvessel network was constructed in a microfluidic device. This functional 3D micro-tissue partly mimicked the physiological response of human vessels. Intravascular nanoparticles tend to adsorb proteins to form a protein corona. Based on this pathological response, vessel permeability and vasoconstriction resulting from ANP stimulation might be related to vascular inflammation. It mediated abnormal expression of nuclear factor-κB (NF-κB) and an influx of intracellular Ca2+ ([Ca2+]i). This biological behavior disturbed the normal expression of intercellular cell adhesion molecule 1 (ICAM-1) and vascular endothelial growth factor (VEGF). The imbalance of nitric oxide (NO) and endothelin-1 (ET-1) further resulted in endothelial cell contraction. All these bio-events induced the loss of tight junctions (ZO-1) which enhanced vessel permeability. Meanwhile, ANP induced-vascular toxicity was also found in mice. Our observations provide a plausible explanation for how the ANPs affect human vascular function. The vessel-on-chip provides a bridge between in vitro results and human responses. We aimed to use this human 3D functional microvascular model to mimic the physiological responses of human vessels. This model is suitable for the evaluation of vascular toxicity after the human vessel exposure to ANPs. © The Royal Society of Chemistry.

语种： 英文

作者： Liu, Yan*;Wang, Yixin;Xia, Zhenzhen;Wang, Yingjie;Wu, Yongning;...

期刊： SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2019年211:336-341 ISSN：1386-1425

通讯作者： Liu, Yan

作者机构： [Liu, Yan; Wang, Yingjie; Wang, Yixin; Gong, Zhiyong; Wu, Yongning] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Hubei, Peoples R China.;[Liu, Yan; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yan; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： Near-infrared spectroscopy;Partial least squares;Phytosterol;Vegetable oil;Wavelength selection

摘要： Phytosterols have been extensively studied because it plays essential roles in the physiology of plants and can be used as nutritional supplement to promote human health. We use a rapid method by coupling near-infrared spectroscopy (NIRS) and chemometric techniques to quickly and efficiently determine three essential phytosterols (beta-sitosterol, campesterol and stigmasterol) in vegetable oils. Continuous wavelet transform (CWT) method was adopted to remove the baseline shift in the spectra. The quantitative analysis models were constructed by partial least squares (PLS) regression and randomization test (RT) method was used to further improve the models. The optimized models were used to calculate the phytosterol contents in prediction set in order to evaluate their predictability. We have found that the phytosterol contents obtained by the optimized models and Gas Chromatography/Mass Spectrometry (GC/MS) analysis are almost consistent. The root mean square error of prediction (RMSEP) and ratio of prediction to deviation (RPD) for the three phytosterols are 525.7590, 212.2245, 65.1611 and 4.0060, 4.7195 and 3.5441, respectively. The results have proved the feasibility of the proposed method for rapid and non-destructive analysis of phytosterols in edible oils.

语种： 英文

作者： Liu, Yan*;Xu, Hao;Xia, Zhenzhen;Gong, Zhiyong

期刊： ANALYST,2018年143(5):1274-1280 ISSN：0003-2654

通讯作者： Liu, Yan

作者机构： [Liu, Yan; Xu, Hao; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Hubei, Peoples R China.

通讯机构： [Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.

摘要： Calibration transfer is indispensable for practical applications of near infrared (NIR) spectroscopy due to the need for precise and consistent measurements across different spectrometers. In this work, a method for multi-spectrometer calibration transfer is described based on independent component analysis (ICA). A spectral matrix is first obtained by aligning the spectra measured on different spectrometers. Then, by using independent component analysis, the aligned spectral matrix is decomposed into the mixing matrix and the independent components of different spectrometers. These differing measurements between spectrometers can then be standardized by correcting the coefficients within the independent components. Two NIR datasets of corn and edible oil samples measured with three and four spectrometers, respectively, were used to test the reliability of this method. The results of both datasets reveal that spectra measurements across different spectrometers can be transferred simultaneously and that the partial least squares (PLS) models built with the measurements on one spectrometer can predict that the spectra can be transferred correctly on another.

语种： 英文

作者： 郭亚丽;程科;王辉;张朝富;李志方;...

期刊： 粮食与饲料工业,2018年(9):1-4 ISSN：1003-6202

作者机构： 国粮武汉科学研究设计院有限公司,湖北武汉,430079;武汉轻工大学食品科学与工程学院,湖北武汉,430023;[李志方; 谢健; 左家瑞; 张朝富; 程科; 杨会宾; 王辉; 郭亚丽] 国粮武汉科学研究设计院;[刘英] 武汉轻工大学

关键词： 重组米;原配料;生产工艺;食用品质;改良剂;应用前景

摘要： 重组米是以碎米、米粉或其他淀粉基质类原料为主要成分,经粉碎、调质后进入挤压机重新制粒、干燥而得。重组米与普通大米相比,在营养强化、成本控制等方面具有显著优势。国内外科研工作者对重组米的研究较为丰富,得到大量研究成果。主要从原配料、生产工艺、品质特性及改良剂等方面论述了近年来国内外重组米领域的相关研究,以期为重组米新产品的开发提供参考。

语种： 中文

作者： Huang, Fei;Liu, Yang;Zhang, Ruifen;Dong, Lihong;Yi, Yang;...

期刊： International Journal of Biological Macromolecules,2018年112:968-975 ISSN：0141-8130

通讯作者： Zhang, Mingwei

作者机构： [Deng, Yuanyuan; Dong, Lihong; Wei, Zhencheng; Huang, Fei; Liu, Yang; Zhang, Ruifen; Zhang, Mingwei] Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Key Lab Funct Foods, Sericultural & Agrifood Res Inst,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Wang, Guangjin] Hubei Engn Univ, Coll Chem & Mat Sci, Xiaogan 432000, Peoples R China.;[Zhang, Mingwei] Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Guangdong, Peoples R China.

通讯机构： [Zhang, Mingwei] G;Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Guangdong, Peoples R China.

关键词： Litchi pulp;Polysaccharide;theological properties

摘要： Litchi polysaccharide (IP) was extracted from litchi pulp. Its chemical composition, microstructure, zeta potential, flow and viscoelastic behavior were investigated. LP contained uronic acid (41.18%), neutral sugar (4223%), and protein (2.72%). The monosaccharide composition was mostly arabinose, galactose, and a small amount of mannose, rhamnose and glucose. Scanning electron microscopy (SEM) showed IP was porous network structure. LP concentration had no effect on its zeta potential value while salts reduced them. LP showed shear-thinning behavior during the tested shear rate range. The power-law model was used to evaluate the flow behavior of LP; both its flow behavior index and consistency index changed with different concentrations. The viscosity of LP increased under acidic conditions (pH 2-4), but was stable with heat treatment. The LP dispersion displayed as a liquid viscoelastic behavior in 1% and 2% concentrations and behaved as an elastic gel at 3% concentration as well as the addition of NaCl and CaCl2. (C) 2018 Published by Elsevier B.V.

语种： 英文

作者： 许诗尧;李芳;胡忠泽;刘刚;秦新光;...

期刊： 中国粮油学报,2017年32(6):1-7 ISSN：1003-0174

通讯作者： Liu, Y.

作者机构： [许诗尧; 李芳; 胡忠泽; 刘刚; Qing X.] Food Science College of Wuhan Polytechnic University, Wuhan, 430023, China;Hubei Rice Engineering and Technology Research Center, Wuhan, 430023, China;[刘英] Food Science College of Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Hubei Rice Engineering and Technology Research Center, Wuhan, 430023, China

通讯机构： Food Science College of Wuhan Polytechnic University, Wuhan, China

关键词： 大米粉;抗性淀粉;水热处理

摘要： 以大米粉为原料,研究压热法、湿热法、韧化法处理对大米粉及其抗性淀粉成分微观结构、结晶学和热力学性质的影响。研究结果表明,压热处理后,大米粉及其抗性淀粉晶型由A型转化为特殊B型,抗性淀粉在20 = 12.9。、19.8。出现2个特征峰象征脂质淀粉复合物的形成;湿热处理后,大米粉形成有一定淀粉酶抗性的外壳结构,晶型转向A型,升温在115 ℃附近出现巨大的特征吸热峰;韧化处理后形成了粒度较大但内部疏松的淀粉团粒。表明压热处理后,大米粉及其抗性淀粉形成特殊晶体结构,晶体和热稳定性均提高;湿热处理对大米粉结晶学和热力学性质有一定影响,但对抗性淀粉影响不大;韧化处理对大米粉及抗性淀粉结晶和热力学性质影响不大。

语种： 中文

作者： Li, Shuyi*;Liu, Yang;Liu, Gang*;He, Jingren;Qin, Xinguang;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017年65(9):1893-1899 ISSN：0021-8561

通讯作者： Li, Shuyi;Liu, Gang

作者机构： [Liu, Gang; Hu, Zhongze; Li, Shuyi; Qin, Xinguang; Lamikanra, Olusola; Liu, Yang; Li, SY; He, Jingren] Wuhan Polytechn Univ, Coll Food Sci & Engn, Wuhan 430023, Hunan, Peoples R China.;[Yang, Haochen] Tianjin Univ, Coll Chem Engn, Tianjin 300072, Peoples R China.

通讯机构： [Li, SY; Liu, G] W;Wuhan Polytechn Univ, Coll Food Sci & Engn, Wuhan 430023, Hunan, Peoples R China.

关键词： Biochemistry;Dimers;Metabolism;Metabolites;Oligomers;Pharmacokinetics;Physiology;Rating;Rats;Degree of polymerization;Gastrointestinal systems;Intestinal metabolisms;Metabolite profiles;Microbial metabolism;Procyanidin oligomers;Quantitative structure activity relationship;Sprague-Dawley rats;Flavonoids;biflavonoid;catechin;plant extract;proanthocyanidin;procyanidin;animal;chemical structure;chemistry;fruit;intestine;lychee;male;metabolism;polymerization;rat;Sprague Dawley rat;Animals;Biflavonoids;Catechin;Fruit;Intestines;Litchi;Male;Molecular Structure;Plant Extracts;Polymerization;Proanthocyanidins;Rats;Rats, Sprague-Dawley

摘要： The bioavailability of A-type procyanidins in vivo has been rarely investigated; as such, this study discusses the effect of A-type linkage and degree of polymerization on the metabolism of procyanidins extracted from litchi pericarp (LPOPC). Sprague-Dawley rats were gavaged with (−)-epicatechin (EC) and LPOPC and sacrificed at different time points after ingestion. A-type linkage procyanidin oligomers inhibited the absorption of EC. Analysis of urinary contents from rats administered with EC, A-type procyanidin dimer (A-2), and A-type procyanidin trimer (A-3) showed distinct native and metabolite profiles for each rat. Rats fed with A-2 and A-3 presented significantly higher levels of shikimic acid and less amount of m(p)-coumaric acid metabolites in vivo and provide insight into the quantitative structure-activity relationship of procyanidin oligomers during metabolism, indicating that procyanidins with A-type linkage could induce an altered metabolic pathway of oligomers in the gastrointestinal system. © 2017 American Chemical Society.

语种： 英文

作者： 李芳;郭亚丽;朱亚军;刘英

期刊： 粮食与饲料工业,2016年(3):35-39 ISSN：1003-6202

作者机构： 武汉轻工大学食品科学与工程学院，湖北 武汉 430023;农产品加工湖北省协同创新中心，湖北 武汉 430023;武汉轻工大学食品科学与工程学院,湖北 武汉,430023;[朱亚军; 李芳; 刘英; 郭亚丽] 武汉轻工大学

关键词： 大豆蛋白;热处理;乳液;稳定性

摘要： 以大豆蛋白粉为主要原料制备未变性及变性大豆蛋白乳液,利用了激光粒度仪法、静置观察法和分层系数法,研究热变性处理对乳液稳定性的影响。实验结果表明：大豆油体积分数为4%,蛋白质的质量浓度为6 mg/ml,90℃热处理10 min 后制得的大豆蛋白乳液粒径最大,乳液分层系数最小。预热处理温度为80℃,加热时间为5 min 时乳液的分层系数最小。选用乳液加热温度(60、70、90℃)、乳液加热时间(5、10、15 min)、大豆蛋白溶液质量分数(0.2%、0.6%、1.0%)和大豆油体积分数(2%、4%、6%)为主要因素及水平,以乳液分层系数为主要参考指标进行正交试验 L 9(34),制备高稳定性大豆蛋白乳液。结果表明,对大豆蛋白乳液稳定性的影响因素从高到低依次为：大豆蛋白溶液质量分数、大豆油体积分数、加热时间和加热温度。大豆蛋白溶液质量分数为1.0%,大豆油体积分数为6%,90℃下预热处理大豆蛋白溶液5 min,得到的乳液的分层系数最小,为35.2%。

语种： 中文

作者： Huang, Fei;Zhang, Ruifen;Liu, Yang;Xiao, Juan;Liu, Lei;...

期刊： International Journal of Biological Macromolecules,2016年92:1067-1073 ISSN：0141-8130

通讯作者： Zhang, Mingwei;Liu, Dong

作者机构： [Wei, Zhencheng; Huang, Fei; Liu, Yang; Xiao, Juan; Zhang, Ruifen; Liu, Lei; Zhang, Mingwei] Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Sericultural & Agri Food Res Inst, Key Lab Funct Foods,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.;[Liu, Dong] Shenzhen Polytech, Shenzhen Key Lab Fermentat Purificat & Anal, Shenzhen 518055, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Liu, Dong] Shenzhen Polytech, Coll Appl Chem & Biotechnol, Shenzhen 518055, Peoples R China.

通讯机构： [Zhang, Mingwei] G;[Liu, Dong] S;Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Sericultural & Agri Food Res Inst, Key Lab Funct Foods,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.;Shenzhen Polytech, Coll Appl Chem & Biotechnol, Shenzhen 518055, Peoples R China.

关键词： Litchi pulp polysaccharides;Immunomodulatory activity;Antioxidant activity

摘要： The purpose of this study was, to investigate the immunomodulatory and antioxidant activities of litchi pulp polysaccharides (LP) in cyclophosphamide (Cy)-induced mice. The administration of LP (50, 100 and 200 mg/kg/d) remarkably enhanced mesenteric lymph node cells proliferation and serum IgA. Moreover, IL-6, TNF-alpha, IgG and IgM levels in serum were significantly improved in a dose-dependent manner with LP treatment. Dietary LP induced systemic immune responses including stimulating the proliferation of splenocytes, balancing the ratio of spleen lymphocyte subsets, up-regulating the thymus and spleen indices. Thus, we speculate litchi polysaccharides would express their systemic immunomodulatory effects by triggering the intestinal mucosal immunity. Likewise, LP also significantly increased total antioxidant capacity, as well as superoxidase dismutase and glutathione peroxidase activity, while decreasing malondialdehyde levels in the serum and liver. The immunomodulatory activity of LP was accompanied with its antioxidant activity, which might be related with its structure and prebiotic effect. This is a novel study on the relationship between the immunomodulatory and antioxidant activities of litchi polysaccharides. (C) 2016 Published by Elsevier B.V.

语种： 英文

作者： 许诗尧;刘英;胡忠泽;刘刚;秦新光;...

期刊： 食品科技,2016年41(5):151-157 ISSN：1005-9989

作者机构： 武汉轻工大学食品科学与工程学院;[李芳] 湖北省稻谷加工工程技术研究中心;[秦新光; 许诗尧; 胡忠泽; 刘刚; 刘英] 武汉轻工大学

关键词： 大米粉;抗性淀粉;水热处理;脂质;透明度;溶解度;膨胀度

摘要： 以大米粉为原料,考查了压热法、湿热法、韧化法处理大米粉对其抗性淀粉含量、基本成分、RVA黏度、透明度、溶解度和膨胀能力的影响。结果表明：压热、湿热、韧化处理均能提高大米粉中抗性淀粉含量。其中压热处理大米粉的抗性淀粉含量最高,可达12.70%。米粉乳浓度、处理温度、处理时间对不同水热处理有不同程度的影响。水热处理前后脂质含量的显著降低说明：在水热处理过程中,脂质参与形成了具有淀粉酶抗性的复合物。压热和湿热大米粉的RVA、透明度变化与大米粉、韧化大米粉差异较大,表明这2种水热处理后淀粉发生老化;压热后大米粉溶解度、膨胀度明显高于湿热、韧化处理,分析压热处理可能对大米粉结构造成影响。

语种： 中文

作者： Song, Zhidan;Liu, Yanchun;Dai, Zhoutong;Liu, Wei;Zhao, Kai;...

期刊： Bioorganic & Medicinal Chemistry,2016年24(19):4723-4730 ISSN：0968-0896

通讯作者： Zhang, Xiuli;Dai, Yujie

作者机构： [Zhang, XL; Dai, Yujie; Zhang, Tongcun; Liu, Yanchun; Zhang, Xiuli; Song, Zhidan; Hu, Yanying] Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Coll Bioengn, Tianjin 300457, Peoples R China.;[Dai, Zhoutong] Wuhan Polytech Univ, Coll Food Sci & Technol, Wuhan 430023, Hubei Province, Peoples R China.;[Liu, Wei] Tianjin Univ Sci & Technol, Coll Sci, Tianjin 300457, Peoples R China.;[Zhao, Kai] Hebei Kingsci Pharmaceut Technol Co Ltd, Shijiazhuang 050035, Hebei Province, Peoples R China.

通讯机构： [Zhang, XL; Dai, YJ] T;Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Coll Bioengn, Tianjin 300457, Peoples R China.

关键词： *4-N-Nitrophenyl substituted amino-4H-1,2,4-triazole;*Aromatase inhibitors;*QSAR

摘要： In this paper, 13 4-N-nitrophenyl substituted amino-4H-1,2,4-triazole derivatives were synthesized and their aromatase inhibitory activities were measured. The results show that the substitution of the groups on benzyl group can further improve their bioactivity and the compound with Cl on the para position of benzyl has the highest bioactivity (IC50 = 9.02 nM). A QSAR model was constructed from the 13 compounds with genetic function approximation using DS 2.1 package. This model can explain 90.09% of the variance (R-Adj(2)), while it can predict 84.95% of the variance (R-cv(2)) with the confidence interval of 95%. (C) 2016 Elsevier Ltd. All rights reserved.

语种： 英文

作者： Huang, Fei;Zhang, Ruifen;Liu, Yang;Xiao, Juan;Su, Dongxiao;...

期刊： Carbohydrate Polymers,2016年152:496-503 ISSN：0144-8617

通讯作者： Zhang, Mingwei

作者机构： [Wei, Zhencheng; Huang, Fei; Liu, Yang; Xiao, Juan; Zhang, Ruifen; Su, Dongxiao; Zhang, Mingwei] Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Key Lab Funct Foods, Minist Agr,Guangdong Key Lab Agr Prod Proc, Guangzhou 510610, Guangdong, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Guangjin] Hubei Engn Univ, Coll Chem & Mat Sci, Xiaogan 432000, Peoples R China.

通讯机构： [Zhang, Mingwei] G;Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Key Lab Funct Foods, Minist Agr,Guangdong Key Lab Agr Prod Proc, Guangzhou 510610, Guangdong, Peoples R China.

关键词： Immunomodulatory activity;Litchi pulp;Mesenteric lymph node cells;Polysaccharide fractions;Structural features

摘要： Three water-soluble hetero-polysaccharides, designated LP1&ndash;3, were isolated from litchi pulp. Their structures, solution properties and immunomodulatory activities were evaluated. LP1 contained (1&nbsp;&rarr;&nbsp;4,6)-&beta;-D-Glc and (1&nbsp;&rarr;&nbsp;4)-&alpha;-L-Gal, while LP2 contained (1&nbsp;&rarr;&nbsp;3)-&alpha;-L-Ara and (l&nbsp;&rarr;&nbsp;2)-&beta;-D-Gal, and LP3 contained &alpha;-L-Ara and (l&nbsp;&rarr;&nbsp;4)-&beta;-Rha. Their molecular weights ranged from 105,880 to 986,470&nbsp;g/mol. LP1 had a spherical conformation with hyper-branched structure and LP2 was semi-flexible chain, while the polysaccharide chains of LP3 were cross linked to form network-like conformation in solution. In addition, all fractions strongly stimulated mesenteric lymph node cell proliferation, IFN-&gamma;and IL-6 secretion in the dose range of 25&ndash;100&nbsp;&mu;g/mL compared with untreated control group (p&nbsp;&lt;&nbsp;0.05). LP1 exhibited the strongest stimulation of mesenteric lymph node cell proliferation and cytokine secretion, which may be attributed to its unique chemical structure and chain conformation. This is the first report on the solution properties and intestinal immunity activities of polysaccharides from litchi pulp.<br/> &copy;2016 Elsevier Ltd

语种： 英文

作者： 郭亚丽;李芳;洪媛;刘英

期刊： 食品科技,2015年40(9):164-168 ISSN：1005-9989

作者机构： 武汉轻工大学食品科学与工程学院;[刘英] 湖北省稻谷加工工程技术研究中心;[李芳; 洪媛; 郭亚丽] 武汉轻工大学

关键词： 模拟米制品;配方;复配比例;综合评分;D-最优混料设计

摘要： 以五丰大米粉、南瓜粉、红薯粉和山药粉为主要原料,采用D-最优混料试验设计方案,研究了不同原料复配比例对模拟米制品综合评分的影响,建立复配比例与模拟米制品综合评分之间的回归模型,考查了配方中各原料的相互效应,获得了最佳模拟米制品配方.具体配方为:大米粉38.10％、南瓜粉6.40％、山药粉32.60％、红薯粉22.80％.

语种： 中文

作者： 郭亚丽;李芳;洪媛;刘英

期刊： 武汉轻工大学学报,2015年(3):1-6 ISSN：2095-7386

作者机构： 武汉轻工大学食品科学与工程学院,湖北武汉,430023;武汉轻工大学食品科学与工程学院，湖北武汉430023;湖北省稻谷加工工程技术研究中心，湖北武汉430023;[李芳; 刘英; 洪媛; 郭亚丽] 武汉轻工大学

关键词： 理化成分;米饭;质构特性;食用品质;相关性

摘要： 在测定8种不同品种大米理化成分的基础上,利用质构仪和SATAKE米饭食味计对米饭样品的质构特性及食用品质进行测定,研究大米理化成分与蒸煮质构特性之间的相关性。结果：原料大米直链淀粉含量、脂肪含量与米饭硬度、胶着性、咀嚼性和回复性呈较显著相关性;原料大米水分含量、直链淀粉含量、脂肪含量、蛋白质含量、灰分对米饭平衡度、口感、外观和综合评分呈较显著相关性。通过理化成分分析,为大米的品质判断提供了科学依据。

语种： 中文

作者： 崔明敏;李芳;刘英

期刊： 粮食加工,2015年40(1):38-42 ISSN：1007-6395

作者机构： 武汉轻工大学食品科学与工程学院;[刘英] 湖北省小麦加工工程技术研究中心;[李芳; 崔明敏] 武汉轻工大学

关键词： 燕麦精粉;燕麦全粉;流变学特性;混合实验仪;挂面

摘要： 以普通小麦粉作为基础,分别添加质量分数为5％、10％、15％、20％、25％、30％、35％的燕麦全粉、燕麦精粉,用Mixolab混合实验仪分析不同添加量燕麦精(全)粉对面团的粉质特性、糊化特性的影响,并通过对面条指数剖面图的分析,从理论上确定混粉中燕麦精(全)粉添加量的优选比例为燕麦全粉质量分数为5％～10％、燕麦精粉质量分数为10％～15％,挂面感官评定验证了该结果.

语种： 中文

作者： 崔明敏;李芳;刘英;董颖颖

期刊： 粮食与饲料工业,2015年(6):28-32 ISSN：1003-6202

作者机构： 武汉轻工大学食品科学与工程学院,湖北武汉,430023;武汉轻工大学食品科学与工程学院，湖北武汉 430023;湖北省小麦加工工程技术研究中心，湖北武汉 430023;[董颖颖; 李芳; 刘英; 崔明敏] 武汉轻工大学

关键词： 燕麦;小麦粉;改良剂;粉质特性;糊化特性;挂面品质

摘要： 使用混合试验仪研究了谷朊粉、海藻酸钠、谷氨酰胺转氨酶（TG酶）、硬脂酰乳酸钠（SSL）、对燕麦‐小麦混合粉面团粉质特性、糊化特性，以及对燕麦挂面的蒸煮特性和感官评价的影响。研究结果表明：四种改良剂均使混合粉面团的稳定时间、形成时间延长，蛋白质弱化度下降，回生值降低；谷朊粉使面团的最低黏度值变大；SSL使面团的糊化峰值黏度值、最低黏度值均变大，使面团的黏度和糊化热稳定性增强；燕麦挂面中加入改良剂后，挂面的吸水率、蛋白质损失率均呈降低趋势，挂面的感官评分基本呈上升趋势，其中SSL和TG酶的改良效果较优，感官评价分值较高。

语种： 中文

作者： 许诗尧;李芳;刘英

期刊： 粮食与食品工业,2015年22(04):8-14 ISSN：1672-5026

作者机构： 武汉轻工大学食品科学与工程学院;[刘英] 湖北省稻谷加工工程技术研究中心;[许诗尧; 李芳] 武汉轻工大学

关键词： 谷物;非化学;抗性淀粉;工艺

摘要： 非化学改性抗性淀粉一直是抗性淀粉研究热点之一。本文对谷物非化学改性抗性淀粉的种类、制备工艺、酶法和添加剂辅助处理等方面的国内外研究进展进行了综述。

语种： 中文

作者： Zhu, Zhenzhou*;Liu, Yan;Guan, Qingyan;He, Jingren;Liu, Gang;...

期刊： Food and Bioprocess Technology,2015年8(8):1680-1689 ISSN：1935-5130

通讯作者： Zhu, Zhenzhou

作者机构： [Liu, Yan; Liu, Gang; Li, Shuyi; Zhu, Zhenzhou; Guan, Qingyan; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Ding, Luhui] Univ Technol Compiegne, Ctr Rech Royallieu, ESCOM, EA TIMR 4297, F-60205 Compiegne, France.;[Jaffrin, Michel Y.] Univ Technol Compiegne, UMR 7338, F-60205 Compiegne, France.

通讯机构： [Zhu, Zhenzhou] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

关键词： Purple sweet potato;Filtration;Separation;Fouling

摘要： Pulsed ultrasound-assisted extraction with water was carried out to extract valuable compounds from purple sweet potato (PSP). Quality properties and dead-end filtration of PSP extract were investigated. Solutes concentration in PSP extract were 118.60 ± 2.83, 599.8 ± 8.28, and 0.72 ± 0.03μgmL−1 for total protein, total polyphenol, and total anthocyanin, respectively. Filtration with PES 50kDa membrane-augmented polyphenol purity from 83.4% in extract to 99.6%. A protein removal coefficient of 99% was achieved. Resistance in series model and Hermia’s model was applied to quantify filtration resistance and investigate fouling mechanism during filtration. Gel layer resistance was the most important for all three used membranes (PES 0.1μm, PES 100kDa, and PES 50kDa). Fitting of experimental data by Hermia’s model revealed that the most suitable fouling mechanism varied, cake layer model for PES 0.1μm and PES 100kDa membrane and intermediate blocking model for PES 50kDa membrane. For the purpose of more efficient polyphenol production, advanced filtration modules, such as cross-flow and rotating disk filtration module, are expected. © 2015, Springer Science+Business Media New York.

语种： 英文