Effect of rhodoxanthin from Potamogeton crispus L. on cell apoptosis in Hela cells
作者：
Ren, Dandan;Peng, Guanghua;Huang, Hongxia;Wang, Haibin;Zhang, Shenghua*
期刊：
Toxicology in Vitro ,2006年20(8):1411-1418 ISSN：0887-2333
通讯作者：
Zhang, Shenghua
作者机构：
[Zhang, Shenghua] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;Hubei Univ Technol, Coll Bioengn, Wuhan 430068, Peoples R China.
通讯机构：
[Zhang, Shenghua] H;Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
关键词：
Antineoplastic Agents;Phytogenic;Apoptosis;Potamogetonaceae;Xanthophylls
摘要：
Carotenoid, a natural functional pigment, is known to have anti-carcinogenic activity. To verify the anti-cancer effects of rhodoxanthin which is a kind of carotenoids, we investigated the effects of rhodoxanthin from Potamogeton crispus L. on the proliferation rate, cell cycle distribution, apoptosis and the change in mitochondrial membrane potential in Hela cell line. The effects of rhodoxanthin were also tested on the concentration of Ca(2+) in cells. Rhodoxanthin inhibited cell proliferation in Hela cells in a dose and time-dependent manner. Rhodoxanthin induced an accumulation of cells in the S phase of the cell cycle, reduced the mitochondria transmembrane potential and increased the concentration of intracellular Ca(2+). In summary, our results suggested that rhodoxanthin-induced apoptosis in Hela cells occurred via these pathways.
语种：
英文
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Cloning and sequence analysis of 2 different acetylcholinesterase genes in Rhopalosiphum padi and Sitobion avenae
作者：
Chen, Maohua;Han, Zhaojun*
期刊：
GENOME ,2006年49(3):239-243 ISSN：0831-2796
通讯作者：
Han, Zhaojun
作者机构：
[Han, Zhaojun] Nanjing Agr Univ, Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing 210095, Peoples R China.;Wuhan Polytech Univ, Food Sci & Engn Coll, Wuhan 430023, Peoples R China.
通讯机构：
[Han, Zhaojun] N;Nanjing Agr Univ, Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing 210095, Peoples R China.
关键词：
Acetylcholinesterase;Aphids;Rhopalosiphum padi (L.);Sitobion avenae (F.)
摘要：
Two genes encoding different acetylcholinesterases (AChE) were successfully cloned from 2 species of aphid, Rhopalosiphum padi (L.) and Sitobion avenue (F.). They were named Rp.AChE1 (GenBank accession No. AY707318), Rp.AChE2 (AY667435), Sa.AChE1 (AY707319), and Sa.AChE2 (AY819704), and were 2133, 2363, 2131, and 2362 bp in length and encoded 664, 676, 664, and 676 amino acids, respectively. All of them shared the characteristics of the AChE family: catalytic tiads, 3 intra-chain disulfide bridges, an acyl pocket, and the conservative aromatic residues for the active site of the gorge. Sequence analysis revealed that Rp.AChE1 and Sa.AChE1 showed higher identity to the reported orthologous genes of Drosophila AChE, and Rp.AChE2 and Sa.AChE2 to paralogous genes. However, in each of the aphids, the 2 genes from the same species shared only 29% identity between one another. It was therefore concluded that each of the aphids has 2 different AChE genes, which are either orthologous or paralogous to Drosophila AChE. The high conservation of AChE1 and AChE2 indicated that 2 acetylcholinesterases exist popularly and that both might function in aphids. © 2006 NRC.
语种：
英文
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高效液相色谱荧光光谱法测定虎杖中的白藜芦醇及其苷的顺、反异构体
作者：
吴波;张寒俊
期刊：
药学学报 ,2006年41(6):522-526 ISSN：0513-4870
通讯作者：
Wu, B.
作者机构：
[张寒俊; 吴波] 武汉工业学院
通讯机构：
Hubei Key Laboratory for Processing and Transformation of Agricultural Product, Wuhan Polytechnic University, China
关键词：
高效液相色谱法;虎杖;异构体;白藜芦醇苷;白藜芦醇;荧光检测器
摘要：
目的 建立高效液相色谱法，分离和测定虎杖中的白藜芦醇及其苷的顺、反异构体的含量。方法 采用Nucleodur 100-5 C18柱（250mm×4．6mm ID，5μm）；流动相为异丙醇和水二元梯度洗脱系统；流速0．6mL·min^-1；荧光检测器激发波长为334nm，发射波长为404nm。结果在选定色谱条件下白藜芦醇及其苷的顺、反异构体线性关系良好。样品加样平均回收率：反式白藜芦醇及其苷分别为96．7％和99．1％；顺式白藜芦醇及其苷分别为91．1％和93．7％。相对标准偏差：反式白藜芦醇及其苷分别为1．34％和0．72％；顺式白藜芦醇及其苷分别为1．27％和2．08％。结论 本方法精密度好、结果可靠，适合虎杖中白藜芦醇及其苷的顺、反异构体定量分析。
语种：
中文
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A New Process for Preparation of Soybean Protein Concentrate with Hexane-Aqueous Ethanol Mixed Solvents
作者：
Zhang, WN* ;Liu, DC
期刊：
JOURNAL OF AOAC INTERNATIONAL ,2005年88(4):1217-1222 ISSN：1060-3271
通讯作者：
Zhang, WN
作者机构：
[Liu, DC; Zhang, WN] Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, WN] W;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
摘要：
A new process for the preparation of soybean protein concentrate (SPC) by directly extracting full-fat soy flour with a mixture of hexane and aqueous ethanol was established. Compared with conventional methods, it has some advantages, such as saving energy and reducing protein denaturation caused by heat action during solvent recovery, because this process saves one step of solvent recovery. The effects of aqueous ethanol concentration and the mixure ratio (hexane to ethanol) on the degree of protein denaturation and product quality were investigated, on the basis of which the orthogonal tests were performed. The optimum technical parameters were obtained by analyzing the results of the orthogonal tests with statistical methods. We found that SPC can be obtained by extracting full-fat soy flour under the following conditions: mixture ratio hexane: 90% ethanol, 9:1, v/v; extraction temperature, 45°C; ratio of solid to solvents, (1:2 w/v); and 5 repeated extractions (15 min each time). The results of quality analysis showed that solubility of the product was improved significantly [nitrogen solubility index (NSI) 46.6%] compared with that for ethanol washing of protein concentrate (NSI 8.7%).
语种：
英文
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菹草类胡萝卜素提取物对人肝癌细胞QGY-7703凋亡的影响
作者：
王海滨;张民;黄进;任丹丹;刘良忠;...
期刊：
分子细胞生物学报：英文版 ,2005年38(1):67-74 ISSN：1674-2788
作者机构：
[王海滨] Lab of Functional Food and Base of Molecular Biology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
关键词：
菹草;类胡萝卜素;肝癌细胞;细胞凋亡;激光扫描共聚焦显微术;流式细胞术
摘要：
分别用含10、20、40、60μmol/L的菹草类胡萝卜素提取物(CEPC)培养液处理人肝癌细胞(QGY-7703)48 h、96 h和144 h,在这三个处理时间各剂量组对肝癌细胞的抑制率平均值范围分别为0.14%-23.07%、39.59%-70.61%和71.65%-87.01%.经10、20和40μmol/L的CEPC培养液处理肝癌细胞24 h、48 h和72 h,用激光扫描共聚焦显微术(LSCM)观察细胞形态,出现了肝癌细胞数量明显减少,细胞体积缩小、皱缩变形,细胞核呈现"新月状"、条状甚至碎片状,细胞核中呈黄色的DNA面积较明显地减小等典型的凋亡细胞形态特征.以流式细胞术分析用CEPC处理肝癌细胞后各时相细胞的百分比,与对照组比较,用10μmol/L和20μmol/L浓度的CEPC处理肝癌细胞48 h后,使细胞周期中的G0/G1期的细胞比例极显著增加(P＜0.01),分别增加了23.8%和35.6%,而在G2/M期没有明显的变化,在S期则相应减少.用LSCM测定了肝癌细胞内的Ca2+浓度,与对照组比较,经20μmol/L CEPC处理48 h后能引起细胞内Ca2+浓度极显著上升(P＜0.01),剂量组细胞内Ca2+荧光强度为对照组的1.5倍.以上结果表明CEPC对人肝癌细胞QGY-7703的增殖具有明显的抑制作用,且在一定程度上呈时间-效应和剂量-效应依赖关系.在较短的时间内及使用较小的CEPC剂量能有效地诱导肝癌细胞凋亡,CEPC使肝癌细胞阻滞于G0/G1期发生凋亡.CEPC能极显著提高肝癌细胞内的Ca2+浓度,提示Ca2+浓度升高可能是CEPC诱导肝癌细胞发生凋亡的重要原因.本项研究结果为进一步研究和开发菹草类胡萝卜素的功能和价值打下了重要基础.
语种：
中文
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High-performance liquid chromatography of some basic drugs on a n-octadecylphosphonic acid modified magnesia-zirconia stationary phase
作者：
He, HB;Feng, YQ* ;Da, SL;Hu, ZX;Qu, L
期刊：
JOURNAL OF SEPARATION SCIENCE ,2005年28(13):1577-1586 ISSN：1615-9306
通讯作者：
Feng, YQ
作者机构：
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan, Peoples R China.
通讯机构：
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
high-performance liquid chromatography;n-octaclecylphosphonic acid modification;magnesia-zirconia;basic drugs
摘要：
The high-performance liquid chromatographic behavior of some basic drugs was studied on a n-octadecylphosphonic acid modified magnesia-zirconia (C<inf>18</inf>PZM) stationary phase. The effect of mobile phase variables such as methanol content, ionic strength, and pH on their chromatographic behavior was investigated. The retention mechanism of basic drugs on the stationary phase was elucidated. The results indicate that both hydrophobic and cation-exchange interactions contribute to solute retention under most chromatographic conditions. The inherent Bro¨nsted-acid sites and also the adsorbed Lewis base anionic buffer constituents on accessible ZM surface Lewis acid sites play a role in the retention of ionized solutes by cation-exchange interaction. However, especially at high mobile phase pH, the retention of basic drugs depends mainly on hydrophobic interactions between solutes and support. Separations of the basic drugs on the C<inf>18</inf>PZM phase by a predominantly reversed-phase retention mode were very promising. The mixed-mode retention feature on this phase, as a result of the adsorbed Lewis base anionic buffer constituents acting as sites for cation-exchange, could also be very useful, e.g. for enhancing the chromatographic selectivity of such analytes. The C<inf>18</inf>PZM seems to be an excellent alternative to silica-based reversed-phase stationary phase for the separation of strongly basic solutes. ©2005 Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim.
语种：
英文
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Magnesia-zirconia based mimetic biomembrane chromatography for predicting human drug absorption
作者：
Hu, ZX;Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2005年827(2):173-181 ISSN：1570-0232
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
Magnesia-zirconia composite;Mimetic biomembrane stationary phase;Oral drug absorption
摘要：
In this paper, a novel mimetic biomembrane chromatography stationary phase of magnesia-zirconia composite matrix were prepared with the Lewis acid-base interaction between phosphatidylcholine's residue phosphonate group and Lewis acid sites of magnesia-zirconia composite;the retention factors of a chemically diverse set of drugs on the new stationary phase were determined;the drugs log K<inf>mbm</inf> values were correlationed with the absorbed fraction of drugs orally administered in humans (%F<inf>a</inf>) and a hyperbolic relationship was obtained. Meanwhile, the relationship between the log K<inf>mbm</inf> values and hydrophobic parameters (log P<inf>oct</inf> and log D<inf>oct</inf>) were discussed. The usefulness of the new column for predicting oral drug absorption in humans is demonstrated by comparing this model with IAM, ILC and BMC models. Results show that the log K<inf>mbm</inf> values have good relationship with logKWIAM, log K<inf>BMC</inf> and have moderate to fair relationship with log K<inf>s</inf> determined on four different ILC column (EPL, PC, PC-PE, PC-PS). Therefore, the log K<inf>mbm</inf> values can provide key information about the transport properties of drugs and this chromatographic model may be applicable for prediction of drug uptake through epithelial cell membranes during the drug discovery process. ©2005 Elsevier B.V. All rights reserved.
语种：
英文
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Optimization of parameters of high-performance displacement chromatography for separation of soybean phosphatidylcholine and phosphatidylethanolamine
作者：
Zhang, WN;Hu, ZX;Feng, YQ;Da, SL*
期刊：
Journal of Chromatography A ,2005年1068(2):269-278 ISSN：0021-9673
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
displacement chromatography;phosphatidylcholine;phosphatidylethanolamine;soybean phospholipids;lipids
摘要：
Hundred milligrams of soybean phospholipids were successfully separated by using high-performance displacement chromatography (HPDC) on a 150 mm x 4.6 mm analytical silica column (3-5 μm packings) with dichloromethane-methanol (9:1, v/v) as carrier and ethanolamine as displacer. From the viewpoint of preparative separation, the effects of loading amount, concentration and flow-rate of displacer on separation efficiency were investigated using throughput and recovery as indices. The parameters were optimized by orthogonal test design and statistical analysis method. Under the optimum conditions, namely displacer concentration being 167 mM, the flow-rate of displacer at 0.2 ml/min and concentration of sample being 211 mg/ml (factual loading amount 211 mg/ml x 0.7 ml = 148 mg), the purity, throughput and recovery of obtained soybean phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were 80.2%, 65.7 mg/h, 70.9% and 90.5%, 272.6 mg/h, 88.3%, respectively. In addition, selections of regenerant and appropriate regeneration condition were also studied. ©2005 Elsevier B.V. All rights reserved.
语种：
英文
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Separation of phosphatidylcholine and phosphatidylethanolamine by using high-performance displacement chromatography
作者：
Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊：
Journal of Chromatography A ,2004年1036(2):145-154 ISSN：0021-9673
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词：
Displacement chromatography;Phosphatidylcholine;Phosphatidylethanolamine;Phospholipids;Lipids
摘要：
A binary mixture of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was successfully separated by high-performance displacement chromatography (HPDC) on an 150 mm x 4.6 turn analytical silica column (3-5 mum packing), using dichloromethane-methanol (9: 1, v/v) as carrier and ethanolamine as displacer. The effects of displacer concentration, flow-rate, loading amount and the composition of the sample on separation efficiency were studied. Eighty-four milligrams sample (PE:PC 1:1.16) was separated perfectly by using 83 mM ethanolamine (in carrier) as displacer at the flow-rate of 0.1 ml/min. The yields of the pure PE and PC (100% purity) were 94.8% and 87.9%, respectively and the cycle time for a single separation was about 195 min. It was valuable that the optimum loading amount (the allowed maximum of sample loading) was investigated only by using the sample to be simulated the composition of the separated actual one, because the separation efficiency was significantly affected by the composition of the sample. For the same loading amount of 175 mg, the yields of the pure PE and PC were improved greatly from 31.4 and 16.9 to 56.0 and 77.6%, respectively, when the proportion of PE to PC was adjusted from 1:1.16 to 1:4. Furthermore, the separation of PE and PC in an actual sample (soybean phospholipids) was achieved using the proposed HPDC method. (C) 2004 Elsevier B.V. All rights reserved.
语种：
英文
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Separation and purification of phosphatidylcholine and phosphatidylethanolamine from soybean degummed oil residues by using solvent extraction and column chromatography
作者：
Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2003年798(2):323-331 ISSN：1570-0232
通讯作者：
Da, SL
作者机构：
[Da, SL] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Da, SL] W;Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China.
关键词：
Phosphatidylcholine;Phosphatidylethanolamine
摘要：
Natural phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were separated and purified from soybean degummed oil residues in this work. Crude PC and PE were first separated from degummed oil residues by extraction with 95% ethanol, and then the crude PC and PE were used as raw materials to prepare high purity PC and PE by using column chromatography of silica gel (100-200 mesh) with different eluents and elution modes. The high purity PC (content >90%) was obtained from the crude PC by using isocratic elution with methanol as eluent. Compared with the methods reported by using isocratic elution with mixed solvents as eluent or gradient elution, the procedure proposed exhibits low cost and industry potentialities because of some advantages, such as operation simplicity, cheap equipment and solvent to be recovered easily. The purity of the PE product prepared from the crude PE was more than 75%. The gradient elution was preferable to isocratic elution for reducing the elution time and eluent consumption when to prepare PE from the crude PE. The effects of loading amount and the flow-rate on separation efficiency were also investigated. For obtaining high separation efficiency, the loading amount should be less than 2.0g crude PC or PE/100g silica gel, and the flow-rate should be controlled under 4ml/min for crude PC and 3ml/min for crude PE, respectively. ©2003 Elsevier B.V. All rights reserved.
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英文
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