Comparison of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides from Fresh and Dried Litchi Pulp
作者：
Huang, Fei;Zhang, Ruifen;Yi, Yang;Tang, Xiaojun;Zhang, Mingwei* ;...
期刊：
Molecules ,2014年19(4):3909-3925 ISSN：1420-3049
通讯作者：
Zhang, Mingwei
作者机构：
[Huang, Fei; Su, Dongxiao] Huazhong Agr Univ, Dept Food Sci & Technol, Wuhan 430070, Peoples R China.;[Deng, Yuanyuan; Wei, Zhencheng; Huang, Fei; Tang, Xiaojun; Zhang, Ruifen; Su, Dongxiao; Zhang, Mingwei] Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Mingwei] G;Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China.
关键词：
litchi;drying;polysaccharides;anti-tumor activity;immunomodulatory activity
摘要：
Drying is commonly used for preservation and processing of litchi. However, its polysaccharide structure may be altered by the drying process, resulting in biological activity changes. Polysaccharides from fresh and dried litchi pulp (denoted as LPF and LPD, respectively) were isolated, investigated by GC-MS, GPC and UV/IR spectrum analysis and their antitumor and immunomodulatory activities were evaluated in vitro. LPD, the molecular weight of which was lower than that of LPF, contained more protein, uronic acid, arabinose, galactose and xylose. Compared with LPF, LPD exhibited a higher inhibitory effect on the proliferation of HepG2, Hela and A549 cells from 50-750 μg/mL. LPD was also a better stimulator of spleen lymphocyte proliferation, NK cells cytotoxicity and macrophage phagocytosis from 50-400 μg/mL. In summary, drying could change the physicochemical properties and enhance the bioactivity of polysaccharides from litchi pulp. This finding is supported by the fact that dried litchi pulps are used in Traditional Chinese Medicine.© 2014 by the authors; licensee MDPI, Basel, Switzerland.
语种：
英文
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ECR-MAPK regulation in liver early development
作者：
Zhao, Xiu-Ju
* （ ;Zhuo, Hexian
期刊：
BioMed Research International ,2014年2014:850802 ISSN：2314-6133
作者机构：
[Zhao, Xiu-Ju] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China.;[Zhuo, Hexian] Xinxiang Inst Drug Control, Xinxiang 453000, Peoples R China.;[Zhao, Xiu-Ju] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
通讯机构：
[Zhao, Xiu-Ju] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
摘要：
Early growth is connected to a key link between embryonic development and aging. In this paper, liver gene expression profiles were assayed at postnatal day 22 and week 16 of age. Meanwhile another independent animal experiment and cell culture were carried out for validation. Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)- ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development. Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations. Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development. Copyright © 2014 X.-J. Zhao and H. Zhuo.
语种：
英文
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应用近红外光谱对低碳数脂肪酸含量预测
作者：
Song Zhi-qiang;Shen Xiong;Zheng Xiao* ;He Dong-ping;Qi Pei-shi;...
期刊：
光谱学与光谱分析 ,2013年33(8):2079-2082 ISSN：1000-0593
通讯作者：
Zheng Xiao
作者机构：
[Shen Xiong; Zheng Xiao; Song Zhi-qiang] Wuhan Polytech Univ, Coll Mech Engn, Wuhan 430023, Peoples R China.;[He Dong-ping] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Qi Pei-shi] PASHUN GRP, Wuhan 430023, Peoples R China.;[Yang Yong; Fang Hui-wen] Wuhan Prod Qual Supervis & Testing Inst, Wuhan 430023, Peoples R China.
通讯机构：
[Zheng Xiao] W;Wuhan Polytech Univ, Coll Mech Engn, Wuhan 430023, Peoples R China.
关键词：
近红外光谱技术;支持向量机;低碳数脂肪酸;粒子群算法;参数优化
摘要：
应用近红外光谱技术结合支持向量机回归（support vector machine regression,SVR）方法测量食用植物油脂低碳数脂肪酸（C≤14）含量。使用SupNIR-5700近红外光谱仪采集58个样品的近红外光谱图,通过偏最小二乘（partial least square,PLS）算法剔除奇异样品。选择其中具有代表性的52个样品进行主成分分析（principal component analysis,PCA）,选取径向基（radial basis function,RBF）核函数建立支持向量机回归模型,并对光谱预处理方法和参数寻优方法进行了详细的分析和讨论。实验表明,经过粒子群算法（parti-cle swarm optimization,PSO）优化后模型的性能都有所提高,泛化能力更强,预测的准确度和稳健性更好;其中预处理方法2经过PSO优化寻优后的参数C=2.085,γ=22.20时,预测集和校正集相关系数（correla-tion coefficient,r）分别达到了0.998 0和0.925 8,均方根误差（root mean square error,MSE）分别为0.000 4和0.014 3。研究结果表明,应用近红外光谱结合PSO-SVR方法进行食用植物油脂低碳数脂肪酸含量快速、准确的预测是可行的。
语种：
中文
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Comparison of analytical methods to assay inhibitors of angiotensin I-converting enzyme
作者：
Chen, Jiwang* ;Wang, Yimei;Ye, Ran;Wu, Yongning;Xia, Wenshui
期刊：
Food Chemistry ,2013年141(4):3329-3334 ISSN：0308-8146
通讯作者：
Chen, Jiwang
作者机构：
[Xia, Wenshui; Wu, Yongning; Wang, Yimei; Chen, Jiwang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Ye, Ran] Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA.;[Wu, Yongning] Chinese Ctr Dis Control & Prevent, Inst Nutr & Food Safety, Beijing 100021, Peoples R China.;[Xia, Wenshui] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.
通讯机构：
[Chen, Jiwang] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Angiotensin I-converting enzyme (ACE);Captopril;High performance liquid chromatography (HPLC) method;Hip-His-Leu (HHL);Visible spectrophotometric (VSP) method
摘要：
The linearity, precision and repeatability of visible spectrophotometric (VSP) and high-performance liquid chromatography (HPLC) methods for analysis of inhibitory activity of angiotensin I-converting enzyme (ACE) were compared by using several inhibitors and Hip-His-Leu (HHL) as substrates. IC50 values (concentration at which ACE activity is inhibited by 50%) of 0.00206 ± 0.00005 μg/mL for captopril, 192 ± 4.53 μg/mL for soybean peptides, and 153 ± 4.29 μg/mL for grass carp peptides determined by the VSP method, and these values were 1.07, 1.07, 1.18 and 1.44-fold, respectively, higher than those from the HPLC method. In addition, the inhibitory constant (Ki value) of captopril was determined to be 7.09 nM and 4.94 nM using VSP and HPLC method, respectively. These results showed that the HPLC method revealed a higher level of sensitivity and precision, suitable for assaying ACE inhibition activity of antihyper-sensitive peptides. In contrast, the VSP method can simultaneously measure several samples with simple operations, suitable for analysis of ACE inhibition activity of food protein enzymatic hydrolysates. © 2013 Elsevier Ltd.
语种：
英文
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A Novel Approach of Periodate Oxidation Coupled with HPLC-FLD for the Quantitative Determination of 3-Chloro-1,2-propanediol in Water and Vegetable Oil
作者：
Hu, Zhixiong;Cheng, Peng;Guo, Mingli;Zhang, Weinong* ;Qi, Yutang
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2013年61(27):6614-6621 ISSN：0021-8561
通讯作者：
Zhang, Weinong
作者机构：
[Qi, Yutang; Guo, Mingli; Zhang, Weinong; Hu, Zhixiong; Cheng, Peng] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Weinong] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
3-chloro-1,2-propanediol (3-MCPD);periodate oxidation;chloroacetaldehyde;high-performance liquid chromatography (HPLC)−fluorescence detector (FLD);water samples;vegetable oil
摘要：
A novel approach of periodate oxidation coupled with high-performance liquid chromatography (HPLC)-fluorescence detection (FLD) for the quantitative determination of 3-chloro-1,2-propanediol (3-MCPD) has been established. The essence of this approach lies in the production of chloroacetaldehyde by the oxidization cleavage of 3-MCPD with sodium periodate and the HPLC analysis of chloroacetaldehyde monitored by an FLD detector after fluorescence derivatization with adenine. The experimental parameters relating to the efficiency of the derivative reaction such as concentration of adenine, chloroacetaldehyde reaction temperature, and time were studied. Under the optimized conditions, the proposed method can provide high sensitivity, good linearity (r<sup>2</sup> = 0.999), and repeatability (percent relative standard deviations between 2.57% and 3.44%), the limits of detection and quantification were 0.36 and 1.20 ng/mL, respectively, and the recoveries obtained for water samples were in the range 93.39-97.39%. This method has been successfully applied to the analysis of real water samples. Also this method has been successfully used for the analysis of vegetable oil samples after pretreatment with liquid-liquid extraction;the recoveries obtained by a spiking experiment with soybean oil ranged from 96.27% to 102.42%. In comparison with gas chromatography or gas chromatography-mass spectrometry, the proposed method can provide the advantages of simple instrumental requirement, easy operation, low cost, and high efficiency, thus making this approach another good choice for the sensitive determination of 3-MCPD. ©2013 American Chemical Society.
语种：
英文
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Simultaneous determination of total fatty acid esters of chloropropanols in edible oils by gas chromatography–mass spectrometry with solid-supported liquid–liquid extraction
作者：
Liu, Qing;Han, Feng;Xie, Ke;Miao, Hong* ;Wu, Yongning
期刊：
Journal of Chromatography A ,2013年1314:208-215 ISSN：0021-9673
通讯作者：
Miao, Hong
作者机构：
[Xie, Ke; Miao, Hong; Wu, Yongning; Liu, Qing] China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China.;[Xie, Ke; Wu, Yongning; Han, Feng] Wuhan Polytech Univ, Dept Food Sci & Engn, Wuhan 430023, Peoples R China.;[Miao, Hong] 7 Panjiayuannanli, Beijing 100021, Peoples R China.
通讯机构：
[Miao, Hong] 7;7 Panjiayuannanli, Beijing 100021, Peoples R China.
关键词：
Chloropropanols esters;Edible oils;Gas chromatography-mass spectrometry (GC-MS);Heptafluorobutyrylim idazole (HFBI);Solid-supported liquid-liquid extraction (SLE)
摘要：
This study aimed to establish a novel robust method for the simultaneous determination of total fatty acid esters of 4 chloropropanols including fatty acid esters of 3-monochloropropane-1,2-diol (3-MCPD esters), 2-monochloropropane-1,3-diol (2-MCPD esters), 1,3-dichloropropan-2-ol (1,3-DCP esters) and 2,3-dichloropropan-1-ol (2,3-DCP esters) in edible oils. In this method, sodium methylate in methanol was used as the reagent for the ester cleavage reaction of chloropropanols esters. The reaction products were extracted by a sodium sulfate solution, and then purified by solid-supported liquid-liquid extraction (SLE) using diatomaceous earth (Hydromatrix™) as the sorbent. Finally, the extracts were derivatized with heptafluorobutyrylim idazole (HFBI) and analyzed by gas chromatography-mass spectrometry (GC-MS). Quantification was achieved using deuterated chloropropanols as their respective internal standards, including 3-MCPD-d<inf>5</inf>, 2-MCPD-d<inf>5</inf>, 1,3-DCP-d<inf>5</inf> and 2,3-DCP-d<inf>5</inf>. A good linear relationship between peak area and concentrations was obtained within the range of 0.025-2.000mgL<sup>-1</sup> with a correlation coefficients not less than 0.999 for all the chloropropanols esters. The limits of detection (LODs) of esters of 3-MCPD, 2-MCPD, 1,3-DCP and 2,3-DCP (calculated as corresponding chloropropanols) were 30, 30, 100 and 30μgkg<sup>-1</sup>, respectively. The average recoveries of the 3-MCPD esters and the 4 chloropropanols spiked at 0.1, 0.5 and 2mgkg<sup>-1</sup> into blank oil matrix were typically in a range from 70.7% to 113.3%. The robust method validation data including calibration, LOD/LOQ, accuracy and repeatability and proficiency test results (Z-score: -0.5) of the official Food Analysis Performance Assessment Scheme (FAPAS) indicated that the present quantitative method could be successfully applied to the determination of total chloropropanols esters in various edible oils. ©2013 .
语种：
英文
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凝胶渗透色谱-固相萃取联合净化气相色谱-质谱联用法测定动物性食品中30种有机氯农药的残留量
作者：
杜娟;吕冰;朱盼;苗虹;吴永宁
期刊：
色谱 ,2013年31(8):739-746 ISSN：1000-8713
作者机构：
武汉工业学院食品科学与工程学院,湖北武汉430023;[朱盼] 卫生部食品安全风险评估重点实验室,国家食品安全风险评估中心,北京100021;[朱盼] 南昌大学食品科学与技术国家重点实验室,江西南昌330031;[吕冰; 苗虹] 国家食品安全风险评估中心;[杜娟; 吴永宁] 武汉工业学院
关键词：
气相色谱-质谱联用;有机氯农药;多残留;动物性食品
摘要：
建立了猪肉、鸡肉、鱼肉和虾肉等动物性食品中30种有机氯农药残留的气相色谱-质谱联用检测方法。样品匀浆后,采用乙腈提取,以凝胶渗透色谱和弗罗里硅土固相萃取柱联合进行净化,气相色谱-质谱检测,以同位素内标法定量。30种有机氯农药的响应在5.0~500.0 μg/L范围内呈良好的线性,相关系数在0.996以上,各有机氯农药的检出限在0.2~2.7 μg/kg之间。以猪肉、鸡肉、鱼肉和虾肉作为代表性基质,进行5.0、10.0、20.0 μg/kg 3个水平的加标回收试验,回收率在55.0%~119.1%之间,相对标准偏差在0.4%~15.0%之间。该方法准确可靠,灵敏度高,样品净化效果好,能够满足动物性食品中有机氯农药多残留痕量分析的要求。
语种：
中文
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High genetic variation among Aschersonia placenta (Clavicipitaceae) isolates from citrus orchards in China
作者：
Wang, P. P.;Song, X. H.;Zhang, H. Y.*
期刊：
Genetics and Molecular Research ,2013年12(4):6192-6202 ISSN：1676-5680
通讯作者：
Zhang, H. Y.
作者机构：
[Zhang, H. Y.; Wang, P. P.; Song, X. H.] Huazhong Agr Univ, Inst Urban & Hort Pests, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China.;[Wang, P. P.] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Song, X. H.] Chongqing Acad Chinese Mat Med, Chongqing, Peoples R China.
通讯机构：
[Zhang, H. Y.] H;Huazhong Agr Univ, Inst Urban & Hort Pests, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China.
关键词：
Aschersonia placenta;China;Genetic diversity;ISSR
摘要：
Aschersonia placenta had been recognized as an important fungal pathogen of whiteflies. In recent years, natural occurrence of Aschersonia in whitefly populations was observed in many citrus orchards in the southern regions of China. We analyzed 60 A. placenta isolates obtained from Chinese citrus orchards, using inter-simple sequence repeats to examine the genetic diversity and to determine whether intraspecific variation is correlated with geographic origin. One hundred and fourteen fragments were generated from these isolates; 97% were polymorphic. The Nei's gene diversity (H) was estimated to be 0.1748 within the populations (range 0.0974-0.2179) and 0.3057 at the species level. Analysis of molecular variance showed that the genetic variation was found mainly within populations (74.9%). The coefficient of gene differentiation (G ST = 0.4315) indicated that 56.85% of the genetic diversity resided within populations. The Mantel test revealed no significant correlation between the genetic distance and the corresponding geographical distance (r = 0.142 and P = 0.887); the unweighted pair-group method using arithmetic average clustering gave similar results. © FUNPEC-RP.
语种：
英文
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Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
作者：
Yi, Yang;Huang, Fei;Zhang, Ming-Wei* ;Zhang, Rui-Fen;Deng, Yuan-Yuan;...
期刊：
Molecules ,2013年18(9):11601-11613 ISSN：1420-3049
通讯作者：
Zhang, Ming-Wei
作者机构：
[Huang, Fei; Zhang, Rui-Fen; Deng, Yuan-Yuan; Zhang, Ming-Wei; Yi, Yang; Wei, Zhen-Cheng] Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Key Lab Funct Food, Guangzhou 510610, Guangdong, Peoples R China.;[Yi, Yang; He, Jing-Ren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Ming-Wei] G;Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Key Lab Funct Food, Guangzhou 510610, Guangdong, Peoples R China.
关键词：
longan pulp;polysaccharide;solution property;conformation;anti-tumor activity
摘要：
The solution properties of four fractions (LPI-IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration <S(2)>(z)((1/2)) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S(2)>(z)((1/2)) = k M(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([eta]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [eta] on M(w) was established to be [eta] = 5.3 x 10(-)(2)M(w)(0).(6)(1) (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII-IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50-400 microg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.
语种：
英文
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离子色谱-串联质谱同时测定蔬菜中的溴酸盐和高氯酸盐
作者：
杨佳佳;杨奕;张晶;邵兵;吴永宁
期刊：
卫生研究 ,2012年41(2):273-278 ISSN：1000-8020
通讯作者：
Yang, J.
作者机构：
[杨佳佳; 吴永宁] 武汉工业学院食品科学与工程学院;[杨奕; 张晶; 邵兵] 北京市疾病预防控制中心
关键词：
离子色谱-串联质谱;溴酸盐;高氯酸盐;蔬菜
摘要：
目的建立同时测定蔬菜中溴酸盐和高氯酸盐的离子色谱-串联质谱(IC-MS/MS)方法。方法蔬菜中的目标离子经水提取,GCB固相萃取小柱净化;采用Ion Pac AS19型亲水性色谱柱,KOH为流动相梯度洗脱,三重四极杆质谱仪作为检测器,ESI负离子模式下,采用MRM采集模式,内标法定量。结果溴酸盐和高氯酸盐分别在0.1~50μg/L和0.05~50μg/L范围内呈良好的线性,相关系数R2>0.998。蔬菜中两种离子的定量限(LOQ)分别为2.5μg/kg和0.7μg/kg。3个浓度水平下,溴酸盐和高氯酸盐在5种蔬菜中的加标回收率为80.3%~124%,日内精密度低于21.4%,日间精密度低于12.2%。结论该方法适用于蔬菜中溴酸盐和高氯酸盐的同时检测。
语种：
中文
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Purification and characterization of a novel angiotensin-I converting enzyme (ACE) inhibitory peptide derived from enzymatic hydrolysate of grass carp protein
作者：
Chen, Jiwang* ;Wang, Yimei;Zhong, Qixin;Wu, Yongning;Xia, Wenshui
期刊：
Peptides ,2012年33(1):52-58 ISSN：0196-9781
通讯作者：
Chen, Jiwang
作者机构：
[Xia, Wenshui; Wu, Yongning; Wang, Yimei; Chen, Jiwang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Zhong, Qixin] Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA.;[Wu, Yongning] Chinese Ctr Dis Control & Prevent, Inst Nutr & Food Safety, Beijing 100021, Peoples R China.;[Xia, Wenshui] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China.
通讯机构：
[Chen, Jiwang] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
ACE-inhibitory peptide;Grass carp protein hydrolysate;Inhibition pattern;Purification;Stability against gastrointestinal enzymes
摘要：
Peptides inhibiting angiotensin-I converting enzyme (ACE, EC. 3.4.15.1) are possible cures of hypertension. Food-derived ACE-inhibitory peptides are particularly attractive because of reduced side effects. Previously, we reported ACE-inhibitory activity of grass carp protein hydrolysates. In this work, we report steps for purifying the ACE-inhibitory peptide from the hydrolysate and its biochemical properties. Following steps of ultrafiltration, macroporous adsorption resin, and two steps of reversed phase high performance liquid chromatography (RE-HPLC), a single Val-Ala-Pro (VAP) tripeptide was identified. The tripeptide with excellent ACE-inhibitory activity (IC50 value of 0.00534 mg/mL) was a competitive ACE inhibitor and stable against both ACE and gastrointestinal enzymes of pepsin and chymotrypsin. This is the first report of food-derived VAP. The identified unique biochemical properties of VAP may enable the application of grass carp protein hydrolysates as a functional food for treatments of hypertension. The developed purification conditions also allow the production of VAP for pharmaceutical applications. (C) 2011 Elsevier Inc. All rights reserved.
语种：
英文
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超高效液相色谱-串联质谱法测定鱼组织中的6种抗雌激素药物
作者：
肖甚圣;杨奕;张晶;吴永宁;邵兵
期刊：
色谱 ,2011年29(11):1055-1061 ISSN：1000-8713
通讯作者：
Wu, Y.
作者机构：
[肖甚圣] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[邵兵; 杨奕; 张晶] Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, China;Institute of Nutrition and Food Safety, Chinese Center for Disease Prevention and Control, Beijing 100025, China;[吴永宁] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China, Institute of Nutrition and Food Safety, Chinese Center for Disease Prevention and Control, Beijing 100025, China
通讯机构：
[Wu, Y.] C;College of Food Science and Engineering, Wuhan Polytechnic University, China
关键词：
固相萃取;超高效液相色谱-串联质谱;抗雌激素药物;鱼肉;鱼肝
摘要：
采用超高效液相色谱-串联质谱(UPLC-MS/MS)在多反应监测(MRM)模式下建立了鱼肉、鱼肝中6种抗雌激素类药物(托瑞米芬、氯米芬、他莫昔芬、雷洛昔芬、阿那曲唑、来曲唑)的检测方法。样品采用乙腈超声提取,上清液中加入适量水稀释提取液,经混合型阳离子交换(MCX)固相萃取柱富集、净化后进行UPLC-MS/MS分析。UPLC分离在ACQUITY UPLC~(TM) BEH C18柱(100 mm×2.1 mm, 1.7 μ m)上进行,以乙腈和0.1%甲酸水溶液为流动相进行梯度洗脱。6种抗雌激素药物的定量限(以信噪比为10计)为0.1~0.3 μ g/kg; 4个加标水平的平均回收率为84.9% ~112.2%,相对标准偏差为0.9% ~14.3% 。该方法可用于鱼肉、鱼肝中6种抗雌激素药物的痕量分析。
语种：
中文
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Application of a modified enzyme-linked immunosorbent assay for 3-amino-2-oxazolidinone residue in aquatic animals
作者：
Liu, Yu;Peng, Dapeng;Huang, Lingli;Wang, Yulian;Chang, Chao;...
期刊：
Analytica Chimica Acta ,2010年664(2):151-157 ISSN：0003-2670
通讯作者：
Yuan, Zonghui
作者机构：
[Wang, Yulian; Liu, Yu; Huang, Lingli; Tao, Yanfei; Ihsan, Awais; Yuan, Zonghui; Peng, Dapeng; Yang, Bo] Huazhong Agr Univ, MOA Key Lab Food Safety Evaluat, Wuhan 430070, Peoples R China.;[Yuan, Zonghui] Huazhong Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residues HZAU, Wuhan 430070, Peoples R China.;[Chang, Chao] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Yuan, Zonghui] Huazhong Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residues HZAU, Shizishan St, Wuhan 430070, Peoples R China.
通讯机构：
[Yuan, Zonghui] H;Huazhong Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residues HZAU, Shizishan St, Wuhan 430070, Peoples R China.
关键词：
3-Amino-2-oxazolidinone;Aquatic animals;Enzyme-linked immunosorbent assay;Furazolidone;Residue
摘要：
Furazolidone has been banned from use in food animals because of its carcinogenicity and mutagenicity, but its continued misuse is widespread in aquacultures. Therefore, there is an urgent need for a simple, reliable, and rapid method for the detection of its marker residue, 3-amino-2-oxazolidinone (AOZ), in aquatic products. In this regard, we modified a simplified indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to address this need. A good linearity was achieved over a concentration range of 0.05-12.15μgL-1, and the IC50 value was 0.96μgL-1. The sample preparation was simple and effective included water bath treatments, acid hydrolysis combined with overnight derivatization of AOZ by benzaldehyde. The limit of detection and the limit of quantification were 0.15 and 0.3μgkg-1. The recoveries of AOZ in all tissues were between 78.0-95.3% at the levels of 0.3, 1.0, and 2.0μgkg-1. The inter-assay variability was less than 19.1%. The modified ic-ELISA was applied in quantification of AOZ elimination in carp. The results showed that AOZ was quite difficult to eliminate. Good correlations of the results obtained by ELISA and LC-MS/MS were observed in incurred carp muscle (r=0.9923) and carp plasma (r=0.9915) at the levels of 2.5-571.8μgkg-1 (μgL-1). Better results were obtained by modified ic-ELISA when compared with commercial ELISA kit. Therefore, the present assay is considered a rapid, accurate, reliable, and inexpensive method for the detection of furazolidone-residues in the edible tissues of aquatic animals. © 2010 Elsevier B.V.
语种：
英文
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Preparation and characterization of phosphatidylcholine-coated zirconia-magnesia stationary phase for artificial membrane chromatography
作者：
Zhang, Weinong* ;Hu, Zhixiong;Feng, Yuqi;Da, Shilu
期刊：
JOURNAL OF SEPARATION SCIENCE ,2010年33(19):2990-2997 ISSN：1615-9306
通讯作者：
Zhang, Weinong
作者机构：
[Zhang, Weinong; Hu, Zhixiong] Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Hubei, Peoples R China.;[Da, Shilu; Feng, Yuqi] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;[Zhang, Weinong] Wuhan Polytech Univ, Dept Food Sci, Xuefu Rd 68, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhang, Weinong] W;Wuhan Polytech Univ, Dept Food Sci, Xuefu Rd 68, Wuhan 430023, Hubei, Peoples R China.
关键词：
Artificial membrane chromatography;Phosphatidylcholine;Stationary phase;Zirconia
摘要：
Immobilized artificial membrane chromatography stationary phase was prepared by coating soybean phosphatidylcholine (PC) on zirconia-magnesia micro-particles. The stability and chromatographic properties were investigated and compared with the PC-coated silica chromatography stationary phase prepared by the same method. PC-coated zirconia-magnesia chromatography stationary phase was more stable than the silica especially on resisting organic solvents. Hydrophobic action was the main factor for the retention of analyte on the new artificial membrane chromatography stationary phase, and electrostatic interaction had some contribution to retention. In addition, the special interaction between analyte and matrix affected retention greatly. Basic solutes were appropriate to be analyzed on PC-coated zirconia-magnesia stationary phase and acidic solutes were appropriate to be done on the silica one. Hence, the two different matrices artificial membrane stationary phases were perfectly complementary. ©2010 WILEY-VCH Verlag GmbH &Co. KGaA, Weinheim.
语种：
英文
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Bioprocess of Triphenylmethane Dyes Decolorization by Pleurotus ostreatus BP Under Solid-State Cultivation
作者：
Yan Keliang;Wang, Hongxun;Zhang, Xiaoyu* ;Yu, Hongbo
期刊：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ,2009年19(11):1421-1430 ISSN：1017-7825
通讯作者：
Zhang, Xiaoyu
作者机构：
[Yan Keliang; Yu, Hongbo; Zhang, Xiaoyu] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;[Wang, Hongxun] Wuhan Polytech Univ, Coll Food Sci & Technol, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Xiaoyu] H;Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.
关键词：
Yan, Keliang;Wang, Hongxun;Zhang, Xiaoyu;Yu, Hongbo;White rot fungus;solid-state fermentation;laccase;dye decolorization
摘要：
With an aim to evaluate dye decolorization by white rot fungus on natural living conditions, reproducing by solidstate fermentation, the process of triphenylmethane dyes decolorization using the white rot fungus P. ostreatus BP, cultivated on rice straw solid-state medium, has been demonstrated. Three typical dyes, including malachite green, bromophenol blue, and crystal violet, were almost completely decolorized by the fungus after 9 days of incubation. During the process of dye decolorization, the activities of enzyme secreted by the fungus, and the contents of soluble components, such as phenolic compounds, protein, and sugar, changed regularly. The fungus could produce ligninolytic, cellulolytic, and hemicellulolytic enzymes and laccase was the most dominant enzyme in solid-state medium. Laccase, laccase isoenzyme, and the laccase mediator could explain the decolorization of malachite green, bromophenol blue, and crystal violet by the fungus in solid-state medium, respectively. It is worth noting that the presence of the water-soluble phenolic compounds could stimulate the growth of fungus, enhance the production of laccase, and accelerate dye decolorization. © The Korean Society and Biotechnology.
语种：
英文
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Composition, Secondary Structure, and Self-Assembly of Oat Protein Isolate
作者：
Liu, Gang;Li, Ji;Shi, Ke;Wang, Su;Chen, Jiwang;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2009年57(11):4552-4558 ISSN：0021-8561
通讯作者：
Huang, Qingrong
作者机构：
[Liu, Gang] S China Univ Technol, Sch Light Chem & Food Sci, Guangzhou 510640, Guangdong, Peoples R China.;[Huang, Qingrong; Shi, Ke; Li, Ji] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA.;[Liu, Gang; Liu, Ying; Chen, Jiwang; Wang, Su] Wuhan Polytech Univ, Dept Food Sci, Wuhan, Hubei Province, Peoples R China.;[Huang, Qingrong] Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA.
通讯机构：
[Huang, Qingrong] R;Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA.
关键词：
Oat protein isolate;amino acid composition;secondary structure;atomic force microscopy;FTIR
摘要：
The amino acid compositions, secondary structure, and self-assembly of oat protein isolate (OPI), which was purified from the high-protein Chinese oat, have been investigated by using a combination of amino acid analysis, Fourier transform infrared spectroscopy (FTIR), and tapping mode atomic force microscopy (TP-AFM). OPI, with molecular weights ranging from 14.0 kDa to 66.0 kDa, was rich in essential amino acids and contained 24.7% glutamic acid and 8.1% leucine. The amino acid contents of OPI are 4.5-8.7 times higher than those of oat flour. The secondary structures of OPI have been quantified by the deconvolution of the amide I band of the FTIR spectrum of OPI, which were found to contain approximately 7% β-tum, 19% α-helix, and 74% β-sheet. Tapping mode AFM results further suggest that the oat protein isolate has two major types of shapes, ellipsoidal and disk-like. At protein concentrations below 0.5 mg/mL, most of the OPI molecules are in the isolated form. However, when the concentration of OPI reaches 1.0 mg/mL, some of the OPI molecules self-assembled into large and heterogeneous protein aggregates. © 2009 American Chemical Society.
语种：
英文
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Biodegradation of crystal violet by low molecular mass fraction secreted by fungus
作者：
Yan, Keliang;Wang, Hongxun;Zhang, Xiaoyu*
期刊：
Journal of Bioscience and Bioengineering ,2009年108(5):421-424 ISSN：1389-1723
通讯作者：
Zhang, Xiaoyu
作者机构：
[Yan, Keliang; Zhang, Xiaoyu] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;[Wang, Hongxun] Wuhan Polytech Univ, Coll Food Sci & Technol, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Xiaoyu] H;Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.
关键词：
Biodegradation;Crystal violet;Decolorization;Fungus;Low molecular mass fraction
摘要：
Biodegradation of crystal violet by white rot fungus Pleurotus ostreatus BP in rice straw solid-state medium mostly depended on low molecular mass fraction (LMMF) and the hypothetical metabolic pathways of crystal violet by LMMF was proposed by the biodegradation of crystal violet and by the deduced six metabolites formed.
语种：
英文
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Determination of florfenicol amine residues in animal edible tissues by an indirect competitive ELISA
作者：
Wu, Jin-E;Chang, Chao* ;Ding, Wen-Ping;He, Dong-Ping
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2008年56(18):8261-8267 ISSN：0021-8561
通讯作者：
Chang, Chao
作者机构：
[Chang, Chao; He, Dong-Ping; Wu, Jin-E; Ding, Wen-Ping] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Chang, Chao] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
florfenicol amine;antibody;ELISA;residue;edible tissues
摘要：
Florfenicol (FF) is a broad-spectrum antibiotic used increasingly in aquaculture, livestock, and poultry to treat diseases. To avoid using labor-intensive instrumental methods to detect residues of FF in food and food products, a simple and convenient indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method for florfenicol's major metabolite, florfenicol amine (FFA), was developed using a polyclonal antibody prepared in this study. FFA was covalently attached to carrier protein as immunogen by using the glutaraldehyde method. The antibodies obtained were characterized by an ELISA method and showed excellent specificity and sensitivity with the 50% inhibition values (IC50) of 3.34 μg/L for FFA in PBS buffer. In the ELISA, sample extractions were performed by ethyl acetate/ammonium hydroxide (90 + 10, v/v) following combined acid hydrolysis of FF and its known metabolites. The limits of detection (LOD) calculated from the analysis of 20 known negative swine muscle, chicken muscle, and fish samples were 3.08,3.3, and 3.86 μg/kg (mean + 3 SD), respectively. Recoveries of FFA fortified at the levels of 5, 50, 100, and 300μg/kg ranged from 64.6 to 124.7%, with coefficients of variation of 11.3-25.8% over the range of FFA concentrations studied. Validation of the ELISA method with FFA-fortified swine muscle at the levels of 10, 50,100, and 200μg/kg was carried out using GC, resulting in a similar correlation in swine muscle (r = 0.97). The results suggest that this ELISA is a specific, accurate, and sensitive method, which is suitable for use as a screening method to detect residues of FFA in animal edible tissues. © 2008 American Chemical Society.
语种：
英文
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锆镁磷脂膜色谱固定相的制备及其在评价药物-膜相互作用中的应用
作者：
胡志雄;张维农;何海波;冯钰锜;达世禄
期刊：
色谱 ,2008年26(5):529-533 ISSN：1000-8713
通讯作者：
Hu, Z.
作者机构：
[冯钰锜; 何海波; 达世禄] College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;Analysis and Test Center, Wuhan Polytechnic University, Wuhan 430023, China;[张维农] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[胡志雄] College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China, Analysis and Test Center, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Hu, Z.] C;College of Chemistry and Molecular Sciences, Wuhan University, China
关键词：
液相色谱;锆镁复合物;模拟生物膜固定相;表观渗透系数;药物-膜相互作用
摘要：
基于锆基质与磷脂之间强烈的路易斯酸碱作用，制备了锆镁磷脂膜色谱固定相，并使用红外光谱、X射线光电子能谱对该色谱固定相进行了表征；使用与体内环境类似的生理缓冲液体系为流动相，评价了该模拟生物膜色谱固定相预测药物膜渗透性的能力，结果表明药物在锆镁磷脂膜色谱中的保留（logK_(mbm)）与表观渗透率（logP_(app)）在预测药物的膜渗透性、跨膜吸收等方面具有非常好的相关性，相关系数为0.970，斜率接近1。通过理论推导，引入了直观、方便的热力学指标吉布斯自由能差值（Δ（ΔG°））对药物-膜之间的相互作用强弱进行了评价。
语种：
中文
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Pretreatment of bamboo residues with Coriolus versicolor for enzymatic hydrolysis
作者：
Zhang, Xiaoyu* ;Xu, Chunyan;Wang, Hongxun
期刊：
Journal of Bioscience and Bioengineering ,2007年104(2):149-151 ISSN：1389-1723
通讯作者：
Zhang, Xiaoyu
作者机构：
[Zhang, Xiaoyu] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;Wuhan Polytech Univ, Coll Food Sci & Technol, Wuhan 430023, Peoples R China.
通讯机构：
[Zhang, Xiaoyu] H;Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.
关键词：
white-rot fungi;biological treatment;fuel ethanol;bamboo
摘要：
Pretreatment by a white-rot fungus Coriolus versicolor B1 under different conditions and saccharification of bamboo were investigated. The saccharification rate was significantly enhanced and a maximum saccharification rate of 37.0% was achieved after pretreatment. Reducing sugars yield was 223.2 mg/g of bamboo residues, which was 2.34 times that of the raw material. It was feasible to treat bamboo residues with B1 for the saccharification of bamboo.
语种：
英文
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