Synthetic surfactant-and cross-linker-free preparation of highly stable lipid-polymer hybrid nanoparticles as potential oral delivery vehicles
作者：
Wang, Taoran;Xue, Jingyi;Hu, Qiaobin;Zhou, Mingyong;Chang, Chao;...
期刊：
Scientific Reports ,2017年7(1):2750 ISSN：2045-2322
通讯作者：
Luo, Yangchao
作者机构：
[Zhou, Mingyong; Chang, Chao; Hu, Qiaobin; Xue, Jingyi; Wang, Taoran; Luo, Yangchao] Univ Connecticut, Dept Nutr Sci, Storrs, CT 06269 USA.;[Chang, Chao] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Luo, Yangchao] U;Univ Connecticut, Dept Nutr Sci, Storrs, CT 06269 USA.
摘要：
The toxicity associated with concentrated synthetic surfactants and the poor stability at gastrointestinal condition are two major constraints for practical applications of solid lipid nanoparticles (SLN) as oral delivery vehicles. In this study, a synthetic surfactant-free and cross-linker-free method was developed to fabricate effective, safe, and ultra-stable lipid-polymer hybrid nanoparticles (LPN). Bovine serum albumin (BSA) and dextran varying in molecular weights were first conjugated through Maillard reaction and the conjugates were exploited to emulsify solid lipid by a solvent diffusion and sonication method. The multilayer structure was formed by self-assembly of BSA-dextran micelles to envelope solid lipid via a pH- and heating-induced facile process with simultaneous surface deposition of pectin. The efficiency of different BSA-dextran conjugates was systematically studied to prepare LPN with the smallest size, the most homogeneous distribution and the greatest stability. The molecular interactions were characterized by Fourier transform infrared and fluorescence spectroscopies. Both nano spray drying and freeze-drying methods were tested to produce spherical and uniform pectin-coated LPN powders that were able to re-assemble nanoscale structure when redispersed in water. The results demonstrated the promise of a synthetic surfactant- and cross-linker-free technique to prepare highly stable pectin-coated LPN from all natural biomaterials as potential oral delivery vehicles.
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英文
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HPLC-DAD-ESI-MS2 analytical profile of extracts obtained from purple sweet potato after green ultrasound-assisted extraction
作者：
Zhu, Zhenzhou;Guan, Qingyan;Koubaa, Mohamed;Barba, Francisco J.* ;Roohinejad, Shahin;...
期刊：
Food Chemistry ,2017年215:391-400 ISSN：0308-8146
通讯作者：
He, Jingren;Barba, Francisco J.
作者机构：
[Li, Shuyi; Zhu, Zhenzhou; Guan, Qingyan; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[Koubaa, Mohamed] Univ Technol Compiegne, Sorbonne Univ, Lab Transformat Integrees Mat Renouvelable,TIMR, Ctr Rech Royallieu,UTC ESCOM,EA 4297,CS 60319, F-60203 Compiegne, France.;[Barba, Francisco J.] Univ Valencia, Fac Pharm Nutr & Food Sci Area, Avda Vicent Andres Estelles S-N, E-46100 Valencia, Spain.;[Roohinejad, Shahin] Univ Otago, Dept Food Sci, POB 56, Dunedin 9054, New Zealand.;[Cravotto, Giancarlo] Univ Turin, Dipartimento Sci & Tecnol Farmaco, Via P Giuria 9, I-10125 Turin, Italy.
通讯机构：
[He, Jingren] W;[Barba, Francisco J.] U;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;Univ Valencia, Fac Pharm Nutr & Food Sci Area, Avda Vicent Andres Estelles S-N, E-46100 Valencia, Spain.
关键词：
Anthocyanins;HPLC-DAD-ESI-MS(2);Polyphenols;Proteins;Purple sweet potato;Ultrasound-assisted extraction
摘要：
Ultrasound pre-treatment (UAE) was applied to assist the extraction of valuable compounds (polyphenols (especially anthocyanins), and proteins) from purple sweet potato (PSP). Under optimum conditions (ultrasound time (40 min);supplementary hot extraction (80 °C) up to 120 min;pH: 2.5;ethanol concentration: 58%), the highest concentrations of polyphenols (3.877 mg/g), anthocyanins (0.293 mg/g), and proteins (0.753 mg/g) were found, with minimal specific energy consumption (8406 J/mg). Moreover, anthocyanin and non-anthocyanin polyphenols in PSP extract from optimized extraction temperature were identified using HPLC-DAD-ESI-MS<sup>2</sup>. The major identified anthocyanins were peonidin-3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, peonidin-3-(6″-caffeoyl-6‴-feruloyl sophoroside)-5-glucoside, cyanidin-3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, whereas the major identified non-anthocyanin molecules were quinic acid, chlorogenic acid, caffeic acid, and chlorogenic acid-3-glucose. The amount of the predominant anthocyanin and non-anthocyanin compounds from PSP extract obtained after UAE was higher than that extracted after conventional solvent extraction. The results obtained in this work demonstrated the efficiency of UAE for the recovery of anthocyanins from PSP.<br/> ©2016 Elsevier Ltd
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英文
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Mass Spectrometric Characterization of an Acid-Labile Adduct Formed with 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and Albumin in Humans
作者：
Wang, Yi;Villalta, Peter W.;Peng, Lijuan;Dingley, Karen;Malfatti, Michael A.;...
期刊：
CHEMICAL RESEARCH IN TOXICOLOGY ,2017年30(2):705-714 ISSN：0893-228X
通讯作者：
Turesky, Robert J.
作者机构：
[Wang, Yi; Turesky, Robert J.; Villalta, Peter W.] Univ Minnesota, Masonic Canc Ctr, Canc & Cardiovasc Res Bldg,2231 6th St, Minneapolis, MN 55455 USA.;[Wang, Yi; Turesky, Robert J.] Univ Minnesota, Dept Med Chem, Canc & Cardiovasc Res Bldg,2231 6th St, Minneapolis, MN 55455 USA.;[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[Dingley, Karen; Turteltaub, K. W.; Malfatti, Michael A.] Lawrence Livermore Natl Lab, Ctr Accelerator Mass Spectrometry, Biosci & Biotechnol Div, Livermore, CA 94550 USA.
通讯机构：
[Turesky, Robert J.] U;Univ Minnesota, Masonic Canc Ctr, Canc & Cardiovasc Res Bldg,2231 6th St, Minneapolis, MN 55455 USA.;Univ Minnesota, Dept Med Chem, Canc & Cardiovasc Res Bldg,2231 6th St, Minneapolis, MN 55455 USA.
摘要：
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a carcinogenic heterocyclic aromatic amine formed during the high-temperature cooking of meats. The cytochrome P450-mediated N-hydroxylation of the exocyclic amine group of PhIP produces 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine, an electrophilic metabolite that forms adducts with DNA and proteins. Previous studies conducted by our laboratory showed that the reaction of N-oxidized PhIP metabolites with human albumin in vitro primarily occurs at the Cys34 residue, to produce an acid-labile linked sulfinamide adduct. On the basis of these findings, we developed a sensitive ultraperformance liquid chromatography-mass spectrometry method to measure acid-labile albumin-PhIP adducts in human volunteers administered a dietary-relevant dose of 14C-labeled PhIP [Dingley, K. H., et al. (1999) Cancer Epidemiol., Biomarkers Prev. 8, 507-512]. Mild acid treatment of albumin (0.1 N HCl, 37 °C for 1 h) or proteolytic digestion with Pronase [50 mM ammonium bicarbonate buffer (pH 8.5) at 37 °C for 18 h] released similar amounts of covalently bound PhIP, which was characterized by multistage scanning and quantified by Orbitrap mass spectrometry. The amount of [14C]PhIP recovered by acid treatment of albumin 24 h following dosing accounted for 7.2-21.3% of the [14C]PhIP bound to albumin based on accelerator mass spectrometry measurements. 2-Amino-1-methyl-6-(5-hydroxy)phenylimidazo[4,5-b]pyridine, a hydrolysis product of the Cys34 S-N linked sulfenamide adduct of PhIP, was not detected in either acid-treated or protease-treated samples. These findings suggest that a portion of the PhIP bound to albumin in vivo probably occurs as an acid-labile sulfinamide adduct formed at the Cys34 residue. © 2016 American Chemical Society.
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英文
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Effect of the A-Type Linkage on the Pharmacokinetics and Intestinal Metabolism of Litchi Pericarp Oligomeric Procyanidins
作者：
Li, Shuyi* ;Liu, Yang;Liu, Gang* ;He, Jingren;Qin, Xinguang;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(9):1893-1899 ISSN：0021-8561
通讯作者：
Li, Shuyi;Liu, Gang
作者机构：
[Liu, Gang; Hu, Zhongze; Li, Shuyi; Qin, Xinguang; Lamikanra, Olusola; Liu, Yang; Li, SY; He, Jingren] Wuhan Polytechn Univ, Coll Food Sci & Engn, Wuhan 430023, Hunan, Peoples R China.;[Yang, Haochen] Tianjin Univ, Coll Chem Engn, Tianjin 300072, Peoples R China.
通讯机构：
[Li, SY; Liu, G] W;Wuhan Polytechn Univ, Coll Food Sci & Engn, Wuhan 430023, Hunan, Peoples R China.
关键词：
A-type procyanidin oligomer;pharmacokinetics;microbial metabolism;rats
摘要：
The bioavailability of A-type procyanidins in vivo has been rarely investigated;as such, this study discusses the effect of A-type linkage and degree of polymerization on the metabolism of procyanidins extracted from litchi pericarp (LPOPC). Sprague-Dawley rats were gavaged with (−)-epicatechin (EC) and LPOPC and sacrificed at different time points after ingestion. A-type linkage procyanidin oligomers inhibited the absorption of EC. Analysis of urinary contents from rats administered with EC, A-type procyanidin dimer (A-2), and A-type procyanidin trimer (A-3) showed distinct native and metabolite profiles for each rat. Rats fed with A-2 and A-3 presented significantly higher levels of shikimic acid and less amount of m(p)-coumaric acid metabolites in vivo and provide insight into the quantitative structure-activity relationship of procyanidin oligomers during metabolism, indicating that procyanidins with A-type linkage could induce an altered metabolic pathway of oligomers in the gastrointestinal system. ©2017 American Chemical Society.
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英文
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Quercetin attenuates high fat diet-induced atherosclerosis in apolipoprotein E knockout mice: A critical role of NADPH oxidase
作者：
Xiao, Lin;Liu, Liang;Guo, Xiaoping;Zhang, Shanshan;Wang, Jing;...
期刊：
Food and Chemical Toxicology ,2017年105:22-33 ISSN：0278-6915
通讯作者：
Tang, Yuhan;Yao, Ping
作者机构：
[Zhou, Feng; Yao, Ping; Tang, YH; Yao, P; Liu, Liegang; Tang, Yuhan; Zhang, Shanshan; Guo, Xiaoping; Xiao, Lin; Wang, Jing] Huazhong Univ Sci & Technol, Sch Publ Hlth, Dept Nutr & Food Hyg, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Zhou, Feng; Yao, Ping; Liu, Liegang; Tang, Yuhan; Zhang, Shanshan; Guo, Xiaoping; Xiao, Lin; Wang, Jing] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Hubei Key Lab Food Nutr & Safety, Wuhan 430030, Peoples R China.;[Zhou, Feng; Yao, Ping; Liu, Liegang; Tang, Yuhan; Zhang, Shanshan; Guo, Xiaoping; Xiao, Lin; Wang, Jing] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Minist Educ,Key Lab Environm, Wuhan 430030, Peoples R China.;[Liu, Liang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Tang, YH; Yao, P] H;Huazhong Univ Sci & Technol, Sch Publ Hlth, Dept Nutr & Food Hyg, Tongji Med Coll, Wuhan 430030, Peoples R China.
关键词：
Quercetin;Atherosclerosis;NADPH oxidase;p47phox;Oxidative stress;Reactive oxygen species
摘要：
Reactive oxygen species (ROS) have emerged as important molecules in cardiovascular function. Nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase is the major source of ROS in phagocytic and vascular cells. Several lines of evidence indicate that quercetin contributes to protecting against atherosclerosis. Herein, we investigated the effect of quercetin on alleviating atherosclerosis by regulating NADPH oxidase subunits expression in vivo, and explored the mechanism of quercetin suppressing the ROS overproduction stimulated by ox-LDL in mouse peritoneal macrophages (MPMs). Model ApoE KO mice were fed with either a normal chow diet or a high fat diet (HFD) supplemented with or without dosed quercetin for 24 weeks. Quercetin significantly reduced the atherosclerotic plaque area, alleviated the systemic oxidative stress, and suppressed aortic p47phox, p67phox expressions but partially reversed the NOX4 expression as compared to those in the HFD group. In vitro, quercetin effectively inhibited the ox-LDL induced ROS formation in MPM5, and blocked the vital step in activation of NADPH oxidase membrane translocation of p47phox. Our findings suggest that regular consumption of dietary quercetin plays a role in preventing atherosclerosis giving its evident regulatory effect on subunits of NADPH oxidase. (C) 2017 Published by Elsevier Ltd.
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英文
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Structural characterization and physicochemical properties of protein extracted from soybean meal assisted by steam flash-explosion with dilute acid soaking
作者：
Zhang, Yanpeng
（ ;Yang, Ruijin
* ;Zhang, Weinong;Hu, Zhixiong;Zhao, Wei
期刊：
Food Chemistry ,2017年219:48-53 ISSN：0308-8146
通讯作者：
Yang, Ruijin
作者机构：
[Zhang, Yanpeng; Zhang, Weinong; Hu, Zhixiong] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhao, Wei; Yang, Ruijin] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.
通讯机构：
[Yang, Ruijin] J;Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.
关键词：
Dilute acid soaking;Physicochemical properties;Soybean protein isolate;Steam flash-explosion;Structural characterization
摘要：
The aim of this work was to analyze the influence of steam flash-explosion (SFE) with dilute acid soaking pretreatment on the structural characteristics and physiochemical properties of protein from soybean meal (SBM). The pretreatment led to depolymerisation of soy protein isolate (SPI) and formation of new protein aggregation through non-disulfide covalent bonds, which resulted in broader MW distribution of SPI. The analysis of CD spectroscopy showed that the SFE treatment induced minor changes in secondary structure, however, the intrinsic tryptophan fluorescence revealed that acid soaking and SFE treatment pronouncedly altered the tertiary structure of SPI. The protein zeta potential was shown to be increased after SFE treatment attributed to the changes in protein structure and the covalent coupling between carbohydrate and protein. These results contribute to clarifying the mechanisms of the effect of pretreatment on SPI structure, thus moving further toward implementing SFE in the processing chain of SPI. © 2016 Elsevier Ltd
语种：
英文
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Protective Effect of Saccharomyces boulardii on Deoxynivalenol-Induced Injury of Porcine Macrophage via Attenuating p38 MAPK Signal Pathway
作者：
Chang, Chao;Wang, Kun;Zhou, Sheng-Nan;Wang, Xue-Dong;Wu, Jin-E*
期刊：
Applied Biochemistry and Biotechnology ,2017年182(1):411-427 ISSN：0273-2289
通讯作者：
Wu, Jin-E
作者机构：
[Chang, Chao; Wu, Jin-E; Wang, Kun; Zhou, Sheng-Nan; Wang, Xue-Dong] Wuhan Polytech Univ, Coll Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
通讯机构：
[Wu, Jin-E] W;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
关键词：
DON;S. boulardii;p38 MAPK;mRNA expression;Cell apoptosis
摘要：
The aims of our study were to evaluate the effects of Saccharomyces boulardii (S. boulardii) on deoxynivalenol (DON)-induced injury in porcine alveolar macrophage cells (PAMCs) and to explore the underlying mechanisms. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, ELISA, qRT-PCR, and western blot were performed to assess whether S. boulardii could prevent DON-induced injury by p38 mitogen-activated protein kinase (p38 MAPK) signal pathway. The results showed that pretreatment with 8μM DON could decrease the viability of PAMC and significantly increase the apoptosis rate of PAMC, whereas S. boulardii could rescue apoptotic PAMC cells induced by DON. Further experiments revealed that S. boulardii effectively reversed DON-induced cytotoxicity via downregulating the expression of TNF-α, IL-6, and IL-lβ. In addition, S. boulardii significantly alleviated DON-induced phosphorylation and mRNA expression of p38 and further increased the expression of apoptosis regulation genes Bcl-xl and Bcl-2 and inhibited the activation of Bax. Our results suggest that S. boulardii could suppress DON-induced p38 MAPK pathway activation and reduce the expression of downstream inflammatory cytokines, as well as promote the expression of anti-apoptotic genes to inhibit apoptosis induced by DON in PAMC. © 2016, Springer Science+Business Media New York.
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英文
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Intervention of pumpkin seed oil on metabolic disease revealed by metabonomics and transcript profile
作者：
Zhao, Xiu-Ju
* （ ;Chen, Yu-Lian;Fu, Bing;Zhang, Wen;Liu, Zhiguo
* ;...
期刊：
Journal of the Science of Food and Agriculture ,2017年97(4):1158-1163 ISSN：0022-5142
通讯作者：
Zhao, Xiu-Ju
（ ;Liu, Zhiguo
作者机构：
[Zhao, Xiu-Ju; Liu, Zhiguo; Chen, Yu-Lian] Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Fu, Bing] Changyuan Cuisine Vocat & Tech Coll, Changyuan 453400, Peoples R China.;[Zhang, Wen] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.;[Zhuo, Hexian] Xinxiang Inst Drug Control Food & Drug Adm Xinxia, 17 Jiankang Rd, Xinxiang 453000, Peoples R China.
通讯机构：
[Zhao, XJ; Liu, ZG] W;Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.
关键词：
pumpkin seed oil (PSO);metabolic disease (MD);metabonomics;transcript profile
摘要：
BACKGROUND: Understanding the metabolic and transcription basis of pumpkin seed oil (PSO) intervention on metabolic disease (MD) is essential to daily nutrition and health. RESULTS: This study analyzed the liver metabolic variations of Wistar rats fed normal diet (CON), high-fat diet (HFD) and high-fat plus PSO diet (PSO) to establish the relationship between the liver metabolite composition/transcript profile and the effects of PSO on MD. By using proton nuclear magnetic resonance spectroscopy together with multivariate data analysis, it was found that, compared with CON rats, HFD rats showed clear dysfunctions of choline metabolism, glucose metabolism and nucleotide and amino acid metabolism. Using quantitative real-time polymerase chain reaction (qPCR), it was found that, compared with HFD rats, PSO rats showed alleviated endoplasmic reticulum stress accompanied by lowered unfolded protein response. CONCLUSION: These findings provide useful information to understand the metabolic alterations triggered by MD and to evaluate the effects of PSO intervention. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry
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英文
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Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities
作者：
Han, Miao-Miao;Yi, Yang* ;Wang, Hong-Xun;Huang, Fei
期刊：
Molecules ,2017年22(6) ISSN：1420-3049
通讯作者：
Yi, Yang
作者机构：
[Wang, Hong-Xun; Han, Miao-Miao; Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Huang, Fei] Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Sericultural & Agrifood Res Inst, Key Lab Funct Foods,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.
通讯机构：
[Yi, Yang] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
longan pulp;polysaccharide;protein;Maillard reaction;physicochemical property;activity
摘要：
The purpose of this study was to investigate the Maillard reaction between polysaccharides and proteins from longan pulp and the effects of reaction on their in vitro activities. The polysaccharide-protein mixtures of fresh longan pulp (LPPMs) were co-prepared by an alkali extraction-acid precipitation method. They were then dry-heated under controlled conditions for monitoring the characterization of the Maillard reaction by the measurement of the free amino group content, ultraviolet-visible spectrum, Fourier transform infrared spectrum and molecular weight distribution. All the physicochemical analyses indicated the development of the Maillard reaction between polysaccharides and proteins. The in vitro activity evaluation indicated that the Maillard reaction could effectively enhance the antioxidant, antitumor and immunostimulating activities of LPPMs. The enhancement of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and ferric reducing antioxidant power displayed both a positive correlation with the reaction time (p < 0.05). LPPMs dry-heated for three days obtained relatively strong inhibitory activity against HepG2 cells and SGC7901 cells, as well as strong immunostimulating effects on the nitric oxide production and tumor necrosis factor alpha secretion of macrophages. Maillard-type intermacromolecular interaction is suggested to be an effective and controllable method for improving the functional activities of polysaccharides and proteins from longan pulp.
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英文
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Recovery of Oil with Unsaturated Fatty Acids and Polyphenols from Chaenomelessinensis (Thouin) Koehne: Process Optimization of Pilot-Scale Subcritical Fluid Assisted Extraction
作者：
Zhu, Zhenzhou;Zhang, Rui;Zhan, Shaoying;He, Jingren* ;Barba, Francisco J.* ;...
期刊：
Molecules ,2017年22(10):22101788-1-22101788-14 ISSN：1420-3049
通讯作者：
He, Jingren;Barba, Francisco J.
作者机构：
[Zhan, Shaoying; Wu, Weizhong; Li, Shuyi; Zhu, Zhenzhou; Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Barba, Francisco J.] Univ Valencia, Fac Pharm, Nutr & Food Sci Area, Prevent Med & Publ Hlth Food Sci Toxicol & Forens, Avda Vicent Andres Estelles S-N, E-46100 Burjassot, Spain.;[Cravotto, Giancarlo] Univ Turin, Dipartimento Sci & Tecnol Farm, Via P Giuria 9, I-10125 Turin, Italy.
通讯机构：
[He, Jingren] W;[Barba, Francisco J.] U;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Univ Valencia, Fac Pharm, Nutr & Food Sci Area, Prevent Med & Publ Hlth Food Sci Toxicol & Forens, Avda Vicent Andres Estelles S-N, E-46100 Burjassot, Spain.
关键词：
Chaenomelessinensis (Thouin) Koehne seed oil;subcritical fluid extraction;response surface methodology;fatty acid composition;polyphenol content
摘要：
The potential effects of three modern extraction technologies (cold-pressing, microwaves and subcritical fluids) on the recovery of oil from Chaenomelessinensis (Thouin) Koehne seeds have been evaluated and compared to those of conventional chemical extraction methods (Soxhlet extraction). This oil contains unsaturated fatty acids and polyphenols. Subcritical fluid extraction (SbFE) provided the highest yield-25.79 g oil/100 g dry seeds-of the three methods. Moreover, the fatty acid composition in the oil samples was analysed using gas chromatography-mass spectrometry. This analysis showed that the percentages of monounsaturated (46.61%), and polyunsaturated fatty acids (42.14%), after applying SbFE were higher than those obtained by Soxhlet, cold-pressing or microwave-assisted extraction. In addition, the oil obtained under optimized SbFE conditions (35 min extraction at 35 degrees C with four extraction cycles), showed significant polyphenol (527.36 mg GAE/kg oil), and flavonoid (15.32 mg RE/kg oil), content, had a good appearance and was of high quality.
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英文
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Direct and indirect measurements of enhanced phenolic bioavailability from litchi pericarp procyanidins by: Lactobacillus casei-01
作者：
Li, Shuyi* ;Li, Xiaopeng;Shpigelman, Avi;Lorenzo, Jose M.* ;Montesano, Domenico;...
期刊：
FOOD & FUNCTION ,2017年8(8):2760-2770 ISSN：2042-6496
通讯作者：
Li, Shuyi;Lorenzo, Jose M.;Barba, Francisco J.
作者机构：
[Li, Shuyi] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Li, Xiaopeng] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Shpigelman, Avi] Technion Israel Inst Technol, Fac Biotechnol & Food Engn, Haifa, Israel.;[Lorenzo, Jose M.] Ctr Tecnol Carne Galicia, C Galicia 4, San Ciprian De Vinas, Ourense, Spain.;[Montesano, Domenico] Univ Perugia, Dipartimento Sci Farmaceut, Sez Sci Alimenti & Nutr, Via S Costanzo 1, Perugia, Italy.
通讯机构：
[Li, Shuyi] W;[Lorenzo, Jose M.] C;[Barba, Francisco J.] U;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Ctr Tecnol Carne Galicia, C Galicia 4, San Ciprian De Vinas, Ourense, Spain.
摘要：
Litchi pericarp procyanidins (LPP) are dietary supplements with high antioxidant activity, but poor oral bioavailability and efficacy. Lactobacillus casei (L. casei-01) can transform flavan-3-ols from litchi pericarp and increase their antioxidant ability; thus, L. casei-01 with LPP was administered to rats for four and eight weeks to study the effect of such a combination on metabolic parameters and on phase II metabolism and detoxification pathways in the liver as an indirect measure for phenolic bioavailability. Our data indicated that the T-AOC of the plasma, the liver GSH-Px and GSH-ST activity, and the expression of UGT and SULT isoforms in the liver of the rats were all enhanced after the eight-week administration compared with those of the control. However, at 1 h after administration the concentration of (-)-epicatechin in the combined system was lower than that obtained after the ingestion of LPP alone, suggesting that L. casei-01 enhances the bioavailability of phenolics from LPP by modulating the transformation to other compounds but not by increasing its absorption in the native form. This journal is © The Royal Society of Chemistry.
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英文
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A SERS-active sensor based on heterogeneous gold nanostar core-silver nanoparticle satellite assemblies for ultrasensitive detection of aflatoxinB1
作者：
Li, Aike* ;Tang, Lijuan;Song, Dan;Song, Shanshan;Ma, Wei;...
期刊：
Nanoscale ,2016年8(4):1873-1878 ISSN：2040-3364
通讯作者：
Li, Aike;Xu, Chuanlai
作者机构：
[Song, Dan; Li, Aike] State Adm Grain, Acad Sci & Technol, Cereals & Oils Nutr Res Grp, Beijing 100037, Peoples R China.;[Tang, Lijuan; Xu, Liguang; Ma, Wei; Xu, Chuanlai; Kuang, Hua; Liu, Liqiang; Wu, Xiaoling; Song, Shanshan] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.;[Chen, Xin] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Li, Aike] S;[Xu, Chuanlai] J;State Adm Grain, Acad Sci & Technol, Cereals & Oils Nutr Res Grp, Beijing 100037, Peoples R China.;Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China.
摘要：
A surface-enhanced Raman scattering (SERS) sensor based on gold nanostar (Au NS) core-silver nanoparticle (Ag NP) satellites was fabricated for the first time to detect aflatoxinB1 (AFB1). We constructed the SERS sensor using AFB1 aptamer (DNA1)-modified Ag satellites and a complementary sequence (DNA2)-modified Au NS core. The Raman label (ATP) was modified on the surface of Ag satellites. The SERS signal was enhanced when the satellite NP was attached to the Au core NS. The AFB1 aptamer on the surface of Ag satellites would bind to the targets when AFB1 was present in the system, Ag satellites were then removed and the SERS signal decreased. This SERS sensor showed superior specificity for AFB1 and the linear detection range was from 1 to 1000 pg mL<sup>-1</sup>with the limit of detection (LOD) of 0.48 pg mL<sup>-1</sup>. The excellent recovery experiment using peanut milk demonstrated that the sensor could be applied in food and environmental detection.<br/> ©The Royal Society of Chemistry 2016.
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英文
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Characterization and preparation of oligomeric procyanidins from Litchi chinensis pericarp
作者：
Sui, Yong;Zheng, Yan;Li, Xiaopeng;Li, Shuyi;Xie, Bijun;...
期刊：
Fitoterapia ,2016年112:168-174 ISSN：1971-551X
通讯作者：
Sun, Zhida
作者机构：
[Sui, Yong; Sun, Zhida; Xie, Bijun; Li, Xiaopeng] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Sui, Yong] Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China.;[Zheng, Yan] Wuhan Inst Design & Sci, Coll Food & Biol Sci & Technol, Wuhan 430205, Peoples R China.;[Li, Shuyi] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Sun, Zhida] H;Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
关键词：
Characterization;Litchi pericarp;Oligomeric procyanidins;Preparation
摘要：
The main purpose of this study is to characterize and prepare A-type oligomeric procyanidins from litchi pericarp (Litchi chinensis Baila). The variety of oligomeric procyanidins was characterized by LC-ESI-MS analysis. There were (+)-catechin, (-)-epicatechin, twelve dimers and six trimers of procyanidins were found in litchi pericarp extracts, and A-type procyanidins were much more abundant than B-type procyanidins. The main flavan-3-ol monomer and oligomeric procyanidins in litchi pericarp were (-)-epicatechin, A-type dimers (A1 and A2) and trimer (epicatechin-(4β-8, 2β-O-7)-epicatechin- (4β-8)-epicatechin). Procyanidin A1 (epicatechin-(4β-8, 2β-O-7)-catechin) was identified by NMR in litchi pericarp for the first time. (-)-Epicatechin and oligomeric procyanidins were prepared by the combination of AB-8 column chromatography and Toyopearl HW-40S column chromatography. The results showed that each fraction predominantly owned a single compound and gave a high yield with (-)-epicatechin, A-type dimers (A1 and A2) and trimer, suggesting a useful method to obtain pure (-)-epicatechin and A-type oligomeric procyanidins. © 2016 Published by Elsevier B.V.
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英文
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Toxicological evaluation of advanced glycation end product Nε-(carboxymethyl)lysine: Acute and subacute oral toxicity studies
作者：
Liu, Xin;Zheng, Liangqing;Zhang, Rong;Liu, Gang;Xiao, Shensheng;...
期刊：
Regulatory Toxicology and Pharmacology ,2016年77:65-74 ISSN：0273-2300
通讯作者：
Gong, Zhiyong
作者机构：
[Liu, Gang; Gong, Zhiyong; Zheng, Liangqing; Qiao, Xiaoting; Liu, Xin; Zhang, Rong; Xiao, Shensheng] Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Wu, Yongning] China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China.;[Gong, Zhiyong] 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Gong, Zhiyong] 6;68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
Mice;Nepsilon-(carboxymethyl)lysine;Oral acute toxicity;Oxidative stress;Subacute toxicity
摘要：
Ne-(carboxymethyl)lysine (CML) as a novel potential noxious compound in various food products has aroused extensive concern in recent years. This study aimed to investigate the oral acute and subacute toxicity of CML in mice as per OECD 420 and 407 guidelines. Acute administration of 2000 and 5000 mg/ kg CML did not induce any mortality within 14 days, nevertheless some toxicological symptoms and histopathological changes were observed. The estimated LD50 of CML was >5000 mg/kg. In subacute toxicity test, CML was dosed at 200, 500 and 1000 mg/kg in both genders for 28 days. The body weights reduced which was accompanied with the decrease of food consumptions. Hematology parameters viz. RBC, HGB and MCH showed minor alteration but these were still within normal range. Biochemical analysis of hepatic and renal function markers showed significant elevating in AST, ALT, Cr and BUN etc. Histopathological alterations were observed in lung, liver, kidney and spleen. Subacute toxicity of CML involved oxidative stress caused by reducing antioxidant enzyme (SOD and GSH-Px) activities, and significantly increasing lipid peroxide (MDA) level. In conclusion, CML was unlikely to present an acute hazard, but repeated administration could produce deleterious effects on mice especially inducing liver and kidney damage through oxidative stress. (C) 2016 Elsevier Inc. All rights reserved.
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英文
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Bioavailability studies and anticancer properties of malvidin based anthocyanins, pyranoanthocyanins and non-oxonium derivatives
作者：
Oliveira, Helder;Wu, Nao;Zhang, Qian;Wang, Jingyi;Oliveira, Joana;...
期刊：
FOOD & FUNCTION ,2016年7(5):2462-2468 ISSN：2042-6496
通讯作者：
He, Jingren;Fernandes, Iva
作者机构：
[Oliveira, Helder; Wang, Jingyi; Zhang, Qian; Wu, Nao; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[de Freitas, Victor; Fernandes, Iva; Oliveira, Helder; Oliveira, Joana; Mateus, Nuno] Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE LAQV, P-4169007 Oporto, Portugal.;[He, Jingren] Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan 430023, Peoples R China.
通讯机构：
[He, Jingren] W;[Fernandes, Iva] U;[He, Jingren] H;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE LAQV, P-4169007 Oporto, Portugal.
摘要：
In this study, the gastric transport efficiency of malvidin-3-glucoside and several derivatives was assayed on the MKN-28 cell model. The transport efficiency was found to increase for all compounds with the incubation time. Pyranoanthocyanins may slightly impair transport efficiency levels in comparison with native anthocyanins. Among the pyranoanthocyanin derivatives the presence of the carbonyl group and the absence of charge were important for the transport efficiency percentage of oxovitisin and apparently compensated the negative effect associated with the additional ring. Moreover, the antiproliferative properties of these compounds in the MCF-7 cancer cell line were assayed, oxovitisin being the most effective compound in inhibiting the proliferation of MCF-7 cells. Also, a kinetic incorporation of oxovitisin was assayed revealing that this pyranoanthocyanin is quickly incorporated into cells. This study confirms the importance of the natural micro-oxidative processes that occur during the ageing of anthocyanin-containing food and their impact on their bioavailability and bioactivity properties. © 2016 The Royal Society of Chemistry.
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英文
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Synthesis of amino-rich silica-coated magnetic nanoparticles for the efficient capture of DNA for PCR
作者：
Bai, Yalong;Cui, Yan;Paoli, George C.;Shi, Chunlei;Wang, Dapeng;...
期刊：
Colloids and Surfaces B: Biointerfaces ,2016年145:257-266 ISSN：0927-7765
通讯作者：
Shi, Xianming
作者机构：
[Zhang, Lida; Zhou, Min; Shi, Xianming; Cui, Yan; Wang, Dapeng; Bai, Yalong; Shi, Chunlei] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China.;[Zhang, Lida; Zhou, Min; Shi, Xianming; Cui, Yan; Wang, Dapeng; Bai, Yalong; Shi, Chunlei] Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Sch Agr & Biol, Shanghai 200240, Peoples R China.;[Zhang, Lida; Zhou, Min; Shi, Xianming; Cui, Yan; Wang, Dapeng; Bai, Yalong; Shi, Chunlei] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China.;[Paoli, George C.] ARS, USDA MOST Joint Res Ctr Food Safety, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA.;[Paoli, George C.] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA.
通讯机构：
[Shi, Xianming] S;Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China.;Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Sch Agr & Biol, Shanghai 200240, Peoples R China.;Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China.
关键词：
Amino-rich;Bio-separation;DNA;Magnetic nanoparticles;PCR
摘要：
Magnetic separation has great advantages over traditional bio-separation methods and has become popular in the development of methods for the detection of bacterial pathogens, viruses, and transgenic crops. Functionalization of magnetic nanoparticles is a key factor for efficient capture of the target analytes. In this paper, we report the synthesis of amino-rich silica-coated magnetic nanoparticles using a one-pot method. This type of magnetic nanoparticle has a rough surface and a higher density of amino groups than the nanoparticles prepared by a post-modification method. Furthermore, the results of hydrochloric acid treatment indicated that the magnetic nanoparticles were stably coated. The developed amino-rich silica-coated magnetic nanoparticles were used to directly adsorb DNA. After magnetic separation and blocking, the magnetic nanoparticles and DNA complexes were used directly for the polymerase chain reaction (PCR), without onerous and time-consuming purification and elution steps. The results of real-time quantitative PCR showed that the nanoparticles with higher amino group density resulted in improved DNA capture efficiency. The results suggest that amino-rich silica-coated magnetic nanoparticles are of great potential for efficient bio-separation of DNA prior to detection by PCR. © 2016.
语种：
英文
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Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties
作者：
Cai, Jie* ;Chen, Jingyao;Zhang, Qian;Lei, Miao;He, Jingren;...
期刊：
Carbohydrate Polymers ,2016年140:238-245 ISSN：0144-8617
通讯作者：
Cai, Jie
作者机构：
[Xiao, Anhong; Cai, Jie; Zhang, Qian; Lei, Miao; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Ma, Chengjie; Li, Sha] Technol Ctr Bright Dairy & Food Co Ltd, State Key Lab Dairy Biotechnol, Shanghai 200436, Peoples R China.;[Cai, Jie; Chen, Jingyao; Xiong, Hanguo] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
通讯机构：
[Cai, Jie] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Acetone (PubChem CID: 180);Cellulose;Composite films;Electrospinning;N,N-Dimethylacetamide (PubChem CID: 31374);Nanofiber;Polyvinyl alcohol (PubChem CID: 11199);Reinforcements
摘要：
Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films. We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites. Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties. The understanding obtained from this study may facilitate the development of novel nanofibrous materials for various optical uses. © 2015 Elsevier Ltd. All rights reserved.
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英文
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Dietary litchi pulp polysaccharides could enhance immunomodulatory and antioxidant effects in mice
作者：
Huang, Fei;Zhang, Ruifen;Liu, Yang;Xiao, Juan;Liu, Lei;...
期刊：
International Journal of Biological Macromolecules ,2016年92:1067-1073 ISSN：0141-8130
通讯作者：
Zhang, Mingwei;Liu, Dong
作者机构：
[Wei, Zhencheng; Huang, Fei; Liu, Yang; Xiao, Juan; Zhang, Ruifen; Liu, Lei; Zhang, Mingwei] Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Sericultural & Agri Food Res Inst, Key Lab Funct Foods,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.;[Liu, Dong] Shenzhen Polytech, Shenzhen Key Lab Fermentat Purificat & Anal, Shenzhen 518055, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Liu, Dong] Shenzhen Polytech, Coll Appl Chem & Biotechnol, Shenzhen 518055, Peoples R China.
通讯机构：
[Zhang, Mingwei] G;[Liu, Dong] S;Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Sericultural & Agri Food Res Inst, Key Lab Funct Foods,Minist Agr, Guangzhou 510610, Guangdong, Peoples R China.;Shenzhen Polytech, Coll Appl Chem & Biotechnol, Shenzhen 518055, Peoples R China.
关键词：
Litchi pulp polysaccharides;Immunomodulatory activity;Antioxidant activity
摘要：
The purpose of this study was, to investigate the immunomodulatory and antioxidant activities of litchi pulp polysaccharides (LP) in cyclophosphamide (Cy)-induced mice. The administration of LP (50, 100 and 200 mg/kg/d) remarkably enhanced mesenteric lymph node cells proliferation and serum IgA. Moreover, IL-6, TNF-alpha, IgG and IgM levels in serum were significantly improved in a dose-dependent manner with LP treatment. Dietary LP induced systemic immune responses including stimulating the proliferation of splenocytes, balancing the ratio of spleen lymphocyte subsets, up-regulating the thymus and spleen indices. Thus, we speculate litchi polysaccharides would express their systemic immunomodulatory effects by triggering the intestinal mucosal immunity. Likewise, LP also significantly increased total antioxidant capacity, as well as superoxidase dismutase and glutathione peroxidase activity, while decreasing malondialdehyde levels in the serum and liver. The immunomodulatory activity of LP was accompanied with its antioxidant activity, which might be related with its structure and prebiotic effect. This is a novel study on the relationship between the immunomodulatory and antioxidant activities of litchi polysaccharides. (C) 2016 Published by Elsevier B.V.
语种：
英文
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Effect of ultrasonication on the fibril-formation and gel properties of collagen from grass carp skin
作者：
Jiang, Ying;Wang, Haibo* ;Deng, Mingxia;Wang, Zhongwen;Zhang, Juntao;...
期刊：
Materials Science and Engineering C: Materials for Biological Applications ,2016年59:1038-1046 ISSN：0928-4931
通讯作者：
Wang, Haibo
作者机构：
[Jiang, Ying; Wang, Zhongwen] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Zhang, Hanjun; Deng, Mingxia; Wang, Haibo; Zhang, Juntao] Wuhan Polytech Univ, Dept Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Wang, Haiyin] Huzhong Univ Sci & Technol, Tongji Med Collage, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wang, Haibo] W;Wuhan Polytech Univ, Dept Chem & Environm Engn, Wuhan, Hubei, Peoples R China.
关键词：
Collagen;Ultrasonic treatment;Fibril formation;Collagen gels;Properties
摘要：
Controlling the fibril-formation process of collagen in vitro to fabricate novel biomaterials is a new area in the field of collagen research. This study aimed to determine the effect of ultrasonication on collagen fibril formation and the properties of the resulting collagen gels. Native collagen, extracted from the skin of grass carp, self-assembled under ultrasonic conditions (at different ultrasonic power and duration). The self-assembly kinetics, fibrillar morphology, and physical and cell growth-promoting properties of the collagen gels were analyzed and compared. The results showed that the self-assembly rate of collagen was increased by ultrasonication at the nucleation stage. The resulting fibrils exhibited smaller diameters and D-periodicity lengths than that of the untreated collagen samples (p < 0.05). The viscoelasticity and textural properties of collagen gels also changed after ultrasonication at the nucleation stage. Texture profile analysis and cell proliferation assays showed that ultrasonication produced softer collagen gel colloids, which were more suitable for cell proliferation than the untreated collagen gels.
语种：
英文
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Enhancement of docosahexaenoic acid production by low-energy ion implantation coupled with screening method based on Sudan black B staining in Schizochytrium sp.
作者：
Fu, Jie;Chen, Tao;Lu, Hao;Lin, Yuanfeng;Xie, Xinlei;...
期刊：
Bioresource Technology ,2016年221:405-411 ISSN：0960-8524
通讯作者：
Zheng, Cao
作者机构：
[He, Dongping; Tian, Hua; Fu, Jie; Lu, Hao; Xie, Xinlei; Lin, Yuanfeng; Zheng, Cao] Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.;[Chen, Tao] Chinese Acad Sci, Wuhan Inst Virol, 44 Xiaohongshan, Wuhan 430071, Hubei, Peoples R China.
通讯机构：
[Zheng, Cao] W;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
DHA;Lipid;Low-energy ion implantation;Schizochytrium sp.;Sudan black B staining
摘要：
Schizochytrium sp. is a hopeful docosahexaenoic acid (DHA) producing candidate due to its rapid growth rate and high DHA proportion in total lipid content. In this study, low-energy ion implantation was applied to Schizochytrium sp. to induce high DHA-producing mutants. Screening these mutants by Sudan black B staining, a mutant strain S1 which showed a 61% improvement in DHA production than that of the parent strain was successfully selected. Subsequently, parameters of DHA production of mutant strain S1 were optimized in a 500-mL Erlenmeyer flask. Under the optimum fermentation conditions, the production of DHA and the percentage of DHA in total lipid of mutant strain S1 were 6.52 g/L and 46.2%, respectively. This study provides an effective breeding strategy for improved DHA production of Schizochytrium sp. through combination of the novel mutagenesis technology, the effective screening method and fermentation optimization. © 2016 Elsevier Ltd
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英文
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