菹草类胡萝卜素提取物对人肝癌细胞QGY-7703凋亡的影响
作者:
王海滨;张民;黄进;任丹丹;刘良忠;...
期刊:
分子细胞生物学报:英文版 ,2005年38(1):67-74 ISSN:1674-2788
作者机构:
[王海滨] Lab of Functional Food and Base of Molecular Biology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
关键词:
菹草;类胡萝卜素;肝癌细胞;细胞凋亡;激光扫描共聚焦显微术;流式细胞术
摘要:
分别用含10、20、40、60μmol/L的菹草类胡萝卜素提取物(CEPC)培养液处理人肝癌细胞(QGY-7703)48 h、96 h和144 h,在这三个处理时间各剂量组对肝癌细胞的抑制率平均值范围分别为0.14%-23.07%、39.59%-70.61%和71.65%-87.01%.经10、20和40μmol/L的CEPC培养液处理肝癌细胞24 h、48 h和72 h,用激光扫描共聚焦显微术(LSCM)观察细胞形态,出现了肝癌细胞数量明显减少,细胞体积缩小、皱缩变形,细胞核呈现"新月状"、条状甚至碎片状,细胞核中呈黄色的DNA面积较明显地减小等典型的凋亡细胞形态特征.以流式细胞术分析用CEPC处理肝癌细胞后各时相细胞的百分比,与对照组比较,用10μmol/L和20μmol/L浓度的CEPC处理肝癌细胞48 h后,使细胞周期中的G0/G1期的细胞比例极显著增加(P<0.01),分别增加了23.8%和35.6%,而在G2/M期没有明显的变化,在S期则相应减少.用LSCM测定了肝癌细胞内的Ca2+浓度,与对照组比较,经20μmol/L CEPC处理48 h后能引起细胞内Ca2+浓度极显著上升(P<0.01),剂量组细胞内Ca2+荧光强度为对照组的1.5倍.以上结果表明CEPC对人肝癌细胞QGY-7703的增殖具有明显的抑制作用,且在一定程度上呈时间-效应和剂量-效应依赖关系.在较短的时间内及使用较小的CEPC剂量能有效地诱导肝癌细胞凋亡,CEPC使肝癌细胞阻滞于G0/G1期发生凋亡.CEPC能极显著提高肝癌细胞内的Ca2+浓度,提示Ca2+浓度升高可能是CEPC诱导肝癌细胞发生凋亡的重要原因.本项研究结果为进一步研究和开发菹草类胡萝卜素的功能和价值打下了重要基础.
语种:
中文
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反相高效液相色谱法测定蛇床子中蛇床子素的含量
作者:
吴波;马长清;张寒俊
期刊:
药物分析杂志 ,2005年25(7):843-845 ISSN:0254-1793
作者机构:
[吴波; 张寒俊] 武汉工业学院;[马长清] 华中科技大学同济医学院药学院药物分析系
关键词:
反相高效液相色谱法;蛇床子素;测定;含量;荧光检测器;加样回收率;线性范围;色谱条件;色谱柱;流动相;波长
摘要:
目的:建立反相高效液相色谱法对蛇床子中蛇床子素的分析方法。方法:色谱柱为BDSHYPERSIL C18(250mm×4.6mm,5μm);流动相:乙醇-水(60:40);流速1mL·min^-1,荧光检测器:激发波长:357nm发射波长:395nm。结果:在选定色谱条件下其蛇床子素的线性范围良好,样品加样回收率为100.6%,RSD为1.8%。结论:该方法测定快速,结果准确、可靠。
语种:
中文
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High-performance liquid chromatography of some basic drugs on a n-octadecylphosphonic acid modified magnesia-zirconia stationary phase
作者:
He, HB;Feng, YQ* ;Da, SL;Hu, ZX;Qu, L
期刊:
JOURNAL OF SEPARATION SCIENCE ,2005年28(13):1577-1586 ISSN:1615-9306
通讯作者:
Feng, YQ
作者机构:
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan, Peoples R China.
通讯机构:
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词:
high-performance liquid chromatography;n-octaclecylphosphonic acid modification;magnesia-zirconia;basic drugs
摘要:
The high-performance liquid chromatographic behavior of some basic drugs was studied on a n-octadecylphosphonic acid modified magnesia-zirconia (C<inf>18</inf>PZM) stationary phase. The effect of mobile phase variables such as methanol content, ionic strength, and pH on their chromatographic behavior was investigated. The retention mechanism of basic drugs on the stationary phase was elucidated. The results indicate that both hydrophobic and cation-exchange interactions contribute to solute retention under most chromatographic conditions. The inherent Bro¨nsted-acid sites and also the adsorbed Lewis base anionic buffer constituents on accessible ZM surface Lewis acid sites play a role in the retention of ionized solutes by cation-exchange interaction. However, especially at high mobile phase pH, the retention of basic drugs depends mainly on hydrophobic interactions between solutes and support. Separations of the basic drugs on the C<inf>18</inf>PZM phase by a predominantly reversed-phase retention mode were very promising. The mixed-mode retention feature on this phase, as a result of the adsorbed Lewis base anionic buffer constituents acting as sites for cation-exchange, could also be very useful, e.g. for enhancing the chromatographic selectivity of such analytes. The C<inf>18</inf>PZM seems to be an excellent alternative to silica-based reversed-phase stationary phase for the separation of strongly basic solutes. ©2005 Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim.
语种:
英文
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Magnesia-zirconia based mimetic biomembrane chromatography for predicting human drug absorption
作者:
Hu, ZX;Zhang, WN;He, HB;Feng, YQ;Da, SL*
期刊:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2005年827(2):173-181 ISSN:1570-0232
通讯作者:
Da, SL
作者机构:
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词:
Magnesia-zirconia composite;Mimetic biomembrane stationary phase;Oral drug absorption
摘要:
In this paper, a novel mimetic biomembrane chromatography stationary phase of magnesia-zirconia composite matrix were prepared with the Lewis acid-base interaction between phosphatidylcholine's residue phosphonate group and Lewis acid sites of magnesia-zirconia composite;the retention factors of a chemically diverse set of drugs on the new stationary phase were determined;the drugs log K<inf>mbm</inf> values were correlationed with the absorbed fraction of drugs orally administered in humans (%F<inf>a</inf>) and a hyperbolic relationship was obtained. Meanwhile, the relationship between the log K<inf>mbm</inf> values and hydrophobic parameters (log P<inf>oct</inf> and log D<inf>oct</inf>) were discussed. The usefulness of the new column for predicting oral drug absorption in humans is demonstrated by comparing this model with IAM, ILC and BMC models. Results show that the log K<inf>mbm</inf> values have good relationship with logKWIAM, log K<inf>BMC</inf> and have moderate to fair relationship with log K<inf>s</inf> determined on four different ILC column (EPL, PC, PC-PE, PC-PS). Therefore, the log K<inf>mbm</inf> values can provide key information about the transport properties of drugs and this chromatographic model may be applicable for prediction of drug uptake through epithelial cell membranes during the drug discovery process. ©2005 Elsevier B.V. All rights reserved.
语种:
英文
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不同粒度玉米超细粉性质的研究
作者:
肖安红;潘从道;王尚玉;张世宏;何东平
期刊:
食品科学 ,2005年26(4):87-91 ISSN:1002-6630
作者机构:
[肖安红; 潘从道; 王尚玉; 张世宏; 何东平] 武汉工业学院食品科学与工程学院;武汉工业学院食品科学与工程学院 湖北武汉430023
关键词:
玉米超细粉;粒度;性质
摘要:
玉米超细粉颗粒97%穿过140目,比面粉(一般为100~120目)粒度小;营养成分重新分布:蛋白质、直链淀粉含量呈下降,总淀粉、灰分呈上升趋势;流变性质发生变化,接近面粉流变性质,且粒度愈小愈易糊化、愈难回生.
语种:
中文
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产多不饱和脂肪酸微生物的研究
作者:
殷梦华;何东平;陈涛
期刊:
Liang you Jiagong (DVD) ,2005年(09):52-55 ISSN:1673-7199
作者机构:
[殷梦华; 何东平; 陈涛] 武汉工业学院食品科学与工程学院
会议名称:
2005北京国际油脂研讨会暨中国粮油学会油脂专业分会成立20周年庆典
会议时间:
2005-10-01
会议地点:
北京
会议论文集名称:
2005'北京国际油脂研讨会暨中国粮油学会油脂专业分会成立20周年庆典(14届年会)论文选集
关键词:
多不饱和脂肪酸;微生物;筛选
摘要:
概述了微生物油脂的研究发展过程,列举了多不饱和脂肪酸(PUFAS)的生理功能、产多不饱和脂肪酸的菌株及其脂肪酸组成的特点。同时,介绍了多不饱和脂肪酸合成途径中的关键酶-脂肪酸脱饱和酶的基因研究情况,以及利用诱变育种技术、抗性筛选法、基因工程筛选和选育高产菌株。
语种:
中文
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菹草类胡萝卜素提取物诱导人肝癌细胞QGY-7703凋亡的细胞学观察
作者:
王海滨;黄进;张民;彭光华;刘良忠;...
期刊:
食品科学 ,2005年26(5):211-216 ISSN:1002-6630
作者机构:
[王海滨; 黄进; 张民; 彭光华; 刘良忠; 任丹丹; 张声华] 华中农业大学食品科技学院功能食品与分子生物学基础研究室;[李忠] 南京医科大学公共卫生学院
关键词:
菹草;类胡萝卜素;肝癌细胞;细胞凋亡;细胞形态
摘要:
采食菹草的蛋鸭所产红心蛋蛋黄的类胡萝卜素提取物具有良好的抑制S180肿瘤的作用,为阐明其抗肿瘤作用机制,研究了菹草类胡萝卜素提取物(CEPC)对人肝癌细胞QGY-7703细胞增殖和凋亡的影响,本文报道采用体外培养、细胞增殖实验,然后以可见光、荧光、激光扫描共聚焦显微镜(LSCM)观察肝癌细胞的形态变化.结果表明:分别用含10、20和40μmol/L的CEPC的培养介质处理QGY-7703细胞24、48和72h,在可见光、荧光显微镜、LSCM下观察细胞形态,分别出现了肝癌细胞数量明显减少、皱缩变形、体积缩小、触角伸长、细胞表面凸起多个小泡,显示亮黄色荧光的细胞核呈现"新月状"、条状甚至碎片状形状,细胞核中呈黄色的DNA面积较明显减小等典型的凋亡细胞形态特征.用荧光显微镜观察计数凋亡细胞的方法测定了CEPC对QGY-7703细胞凋亡指数影响,细胞凋亡指数随CEPC剂量的增加及处理时间的延长而上升.因此CEPC对QGY-7703细胞的增殖具有明显的抑制作用,在较短的时间内及使用较小的CEPC剂量能有效地诱导QGY-7703细胞凋亡.本项研究说明菹草中的类胡萝卜素化合物具有明显的抑制肝癌细胞增殖、诱导肝癌细胞凋亡的功能.
语种:
中文
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尿素包合物固相中回收脂肪酸的工艺研究
作者:
胡小泓;张新才;周临桃;朱小波;邓淑仪
期刊:
食品科学 ,2005年26(11):141-144 ISSN:1002-6630
作者机构:
[胡小泓; 张新才; 周临桃; 朱小波; 邓淑仪] 武汉工业学院食品科学与工程学院;武汉工业学院食品科学与工程学院 湖北武汉430023
关键词:
茶油;亚麻油;尿素包合物固相;回收;脂肪酸
摘要:
采用浸出法、直接酸解法、浸出后酸解法回收茶油和亚麻油尿素包合物固相中的脂肪酸,并将三种回收方法进行了比较.在实验中根据酸的浓度、温度对得率、酸价和碘价的影响进行了分析.通过对包合前、包合后以及包合物固相中脂肪酸成分的色谱分析,得出在尿素包合物固相中,饱和与单不饱和脂肪酸的纯度有了很大提高.茶油中油酸的含量由原来的66.99%上升至70.96%,亚麻油中油酸的含量由原来的15.35%上升至31.75%.实验得出经尿素包合后的脂肪酸,不易氧化.
语种:
中文
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番茄汁饮料品质改良技术研究
作者:
刘志伟;孟立;姜华年
期刊:
食品科学 ,2005年26(7):149-151 ISSN:1002-6630
作者机构:
[刘志伟] 中国疾病预防控制中心营养与食品安全所;[孟立] 浙江丽水学院生物系;[姜华年] 浙江丽水学院辽物系
关键词:
番茄汁饮料;破碎;粘度;稳定性;工艺参数
摘要:
本文探讨了番茄汁饮料加工过程中的几个关键技术点:1.番茄的破碎方法对番茄汁粘度的影响;2.防止产品分层不稳定现象;3.口感的改良及其最佳配方工艺参数的确定.结果表明,采用85℃、30s热破碎榨汁工艺,0.3%茶多酚作护色剂,以8%蔗糖,0.25%柠檬酸,0.13%复合稳定剂,65%原汁含量为配方组成,可得到品质优良的番茄汁饮料产品.
语种:
中文
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油菜籽脱皮冷榨制备菜籽多肽的研究
作者:
郭涛;黄桃菊;韩文忠;何东平
期刊:
中国油脂 ,2005年30(9):15-16 ISSN:1003-7969
作者机构:
[郭涛; 黄桃菊; 韩文忠; 何东平] 武汉工业学院食品科学与工程学院;武汉工业学院食品科学与工程学院 430023武汉市常青花园中环西路特1号
关键词:
油菜籽;脱皮冷榨;菜籽蛋白;酶解;菜籽多肽
摘要:
油菜籽经脱皮冷榨得到冷榨饼,再经低温浸出脱溶得到低温粕,对低温粕提取菜籽蛋白,然后用中性蛋白酶AS1.398水解菜籽蛋白得到菜籽多肽.通过正交实验得到的最佳酶解条件是:pH7.2、温度45℃、加酶量5%(酶与原料液的质量比)、酶解时间2 h,酶解后有75%左右的蛋白质转化为可溶性肽,所得到的菜籽多肽流动性好,黏度低,热稳定性较好.
语种:
中文
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鲜木瓜的贮藏试验
作者:
汪芳安;刘军;王志芳
期刊:
武汉轻工大学学报 ,2005年24(1):8-9 ISSN:2095-7386
作者机构:
[刘军; 汪芳安] 武汉工业学院;[王志芳] 华中农业大学
关键词:
贮藏;木瓜;试验;还原糖;保存;总酸;常温;大批量;适宜;失重
摘要:
介绍了几种适宜于大批量鲜木瓜常温下贮藏的方法.试验表明,采用砂贮法保存的木瓜,其失重较少,还原糖和总酸变化较小.
语种:
中文
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高酸值麻疯籽油甲酯化工艺的研究
作者:
佘珠花;刘大川;潭蓓玉
期刊:
Liang you Jiagong (DVD) ,2005年(09):45-46 ISSN:1673-7199
作者机构:
武汉工业学院农产品加工与转化重点实验室
关键词:
高酸值麻疯籽油;甲酯化;水解;脂肪酸甲酯
摘要:
用直接甲酯化法和水解后酯化法两种工艺对高酸值麻疯籽油的甲酯化进行了探讨.认为直接甲酯化法更容易操作,能耗更小,酯化较彻底,得到的甲酯产品颜色浅.直接甲酯化法应分3步进行:酸催化甲酯化→脱水干燥处理→碱催化甲酯化.这对高酸值油的顺利甲酯化有重要意义.
语种:
中文
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Adsorptive voltammetry of Hg(II) ions at a glassy carbon electrode coated with electropolymerized methyl-red film
作者:
Yang, NJ;Wan, QJ* ;Yu, JH
期刊:
Sensors and Actuators B-Chemical ,2005年110(2):246-251 ISSN:0925-4005
通讯作者:
Wan, QJ
作者机构:
[Wan, QJ] Wuhan Inst Technol, Sch Chem Engn & Pharm, Hubei Key Lab Novel Reactor & Green Chem Technol, Wuhan 430073, Peoples R China.;Univ Fukui, Grad Sch Engn, Fukui 9108507, Japan.;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.
通讯机构:
[Wan, QJ] W;Wuhan Inst Technol, Sch Chem Engn & Pharm, Hubei Key Lab Novel Reactor & Green Chem Technol, Wuhan 430073, Peoples R China.
关键词:
modified electrode;adsorptive voltammetry;electropolymerization;methyl red;Hg(II) ion;ANODIC-STRIPPING VOLTAMMETRY;MODIFIED SOLID ELECTRODES;PASTE ELECTRODES;GOLD ELECTRODE;DISK ELECTRODE;MERCURY;POLYPYRROLE;PRECONCENTRATION;ACID
摘要:
The glassy carbon electrode coated with electropolymerized methyl-red film, 1.2 x 10(-6) m in thickness, (PMRE) showed high sensitivity towards Hg(II) ions. PMREs were adopted to accumulate and detect H(II) ions in a pH 2.56 Britton-Robinson buffer solution. Cyclic voltammogram of the accumulated Hg species on PMREs exhibited an anodic wave at 0.64 V and a cathodic wave at 0.13 V, due to the oxidation of accumulated Hg species on PMREs and the reduction of Hg(II) ions in the solution, respectively. For this heterogeneous adsorption of Hg(11) ions onto PMREs, the maximum surface concentration, adsorption equilibrium, and Gibbs energy change were evaluated to be 5.12 x 10(-6) mol m(-2), 3.7 x 10(5) l mol(-1), and -30.1 kJ mol(-1), respectively. The anodic peak current at 0.64 V was linear with the concentration of Hg(H) ions in the range of 1.1 x 10(-10) to 1.1 x 10(-7) M with a detection limit of 4.4 x 10(-11) M. The proposed method was utilized successfully for the detection of Hg(II) ions in the lake water. (C) 2005 Elsevier B.V. All rights reserved.
语种:
英文
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双低菜籽制备复合氨基酸粉的新工艺研究
作者:
张寒俊;刘大川
期刊:
河南工业大学学报(自然科学版) ,2005年26(5):28-30 ISSN:1673-2383
作者机构:
武汉工业学院,农产品加工与转化重点实验室,湖北,武汉,430023;[刘大川; 张寒俊] 武汉工业学院
关键词:
双低油菜籽;天然复合氨基酸粉;制备工艺
摘要:
以双低油菜籽脱脂粕为原料,采用硫酸高温短时酸解法制备天然复合氨基酸粉,通过正交试验确定最佳工艺参数为:酸解温度130℃,时间2h,硫酸浓度6mol/L,液固比3:1.并对氨基酸液的粗制品采用CaCO3中和、减压蒸发浓缩、冷冻干燥等方法进行精制.经本工艺生产的氨基酸产品色泽为橙红色,香气正常,无异味,鲜咸适口,产品得率为38.70%,氨基氮含量为5.13%,总氮含量为8.28%,总水解度为87.50%.
语种:
中文
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Preparation and evaluation of n-octadecylphosphonic acid-modified magnesia–zirconia stationary phases for reversed-phase liquid chromatography
作者:
He, HB;Feng, YQ* ;Qu-Li;Da, SL;Hu, ZX
期刊:
Analytica Chimica Acta ,2005年542(2):268-279 ISSN:0003-2670
通讯作者:
Feng, YQ
作者机构:
[Feng, YQ] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430072, Peoples R China.
通讯机构:
[Feng, YQ] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词:
Alkaloids;Basic compounds;Magnesia-zirconia;Octadecylphosphonic acid-modified;Reversed-phase liquid chromatography;Stationary phases
摘要:
Three n-octadecylphosphonic acid-modified magnesia-zirconia reversed stationary phases (C18PZM) are prepared via the strong Lewis base interactions between organophosphonate and magnesia-zirconia composite. And two of them are end-capped by using trimethylchlorosilane as end-capping agent in different procedures. Stability studies at extreme high pH conditions (pH 9-12) show that both the non-endcapped and endcapped columns are quite stable at pH 12 mobile phase. The reversed-phase liquid chromatographic behavior of three C18PZM stationary phases are comparatively investigated in detail using a variety of basic compounds as probes. The retention of basic compounds on the three phases is studied over a wide range of pHs. And the possible retention mechanisms of basic compounds on the three stationary phases are discussed. The results show that the basic solutes retain by a hydrophobic and cation-exchange interaction mixed mechanism on three stationary phases when they are operated in eluents at pH values near to the pKa of the Brönsted conjugate acid form of the analyte, suggesting that inherent zirconol groups on ZM are not expected to interact with bases via cation-exchange interaction at lower pH. Nonetheless, the non-endcapped phase differs markedly from the edncapped ones in retention and selectivity of basic solutes using eluents at pH 4.1, implying a complex retention mechanism at this pH. The cation-exchange sites under such conditions are more likely due to the adsorbed Lewis base anionic buffer constituents (acetate) on accessible ZM surface sites than the chemisorbed phosphonate. Although the three phases exhibit very similar chromatographic behavior with eluents at pH 10.1, and show in general satisfactory separation of basic compounds and alkaloids studied, the performance for a specific analyte, however, differs largely from column to column. © 2005 Elsevier B.V. All rights reserved.
语种:
英文
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高效液相色谱法测定食品中齐墩果酸的含量
作者:
吴波;张寒俊
期刊:
食品科学 ,2005年26(12):176-178 ISSN:1002-6630
作者机构:
[吴波; 张寒俊] 武汉工业学院
关键词:
高效液相色谱;齐墩果酸;食品;绿豆;赤豆;莲子;枣
摘要:
采用高效液相色谱法对绿豆、赤豆、莲子、枣中的齐墩果酸进行分离和分析,所用色谱柱为NucleodurC18柱(250mm×4.6mmi.d.,5μm);流动相:80%乙醇;在选定色谱条件下其齐墩果酸的线性范围良好,样品加样回受率在96.7%~101.5%,相对标准偏差在0.64%~2.89%.
语种:
中文
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Optimization of parameters of high-performance displacement chromatography for separation of soybean phosphatidylcholine and phosphatidylethanolamine
作者:
Zhang, WN;Hu, ZX;Feng, YQ;Da, SL*
期刊:
Journal of Chromatography A ,2005年1068(2):269-278 ISSN:0021-9673
通讯作者:
Da, SL
作者机构:
[Da, SL] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.;Wuhan Polytech Univ, Dept Food Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Da, SL] W;Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China.
关键词:
displacement chromatography;phosphatidylcholine;phosphatidylethanolamine;soybean phospholipids;lipids
摘要:
Hundred milligrams of soybean phospholipids were successfully separated by using high-performance displacement chromatography (HPDC) on a 150 mm x 4.6 mm analytical silica column (3-5 μm packings) with dichloromethane-methanol (9:1, v/v) as carrier and ethanolamine as displacer. From the viewpoint of preparative separation, the effects of loading amount, concentration and flow-rate of displacer on separation efficiency were investigated using throughput and recovery as indices. The parameters were optimized by orthogonal test design and statistical analysis method. Under the optimum conditions, namely displacer concentration being 167 mM, the flow-rate of displacer at 0.2 ml/min and concentration of sample being 211 mg/ml (factual loading amount 211 mg/ml x 0.7 ml = 148 mg), the purity, throughput and recovery of obtained soybean phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were 80.2%, 65.7 mg/h, 70.9% and 90.5%, 272.6 mg/h, 88.3%, respectively. In addition, selections of regenerant and appropriate regeneration condition were also studied. ©2005 Elsevier B.V. All rights reserved.
语种:
英文
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菹草颗粒饲料营养成分的分析
作者:
王海滨;任丹丹;张声华
期刊:
华中农业大学学报 ,2005年24(4):410-413 ISSN:1000-2421
作者机构:
[王海滨; 任丹丹; 张声华] 华中农业大学食品科技学院
关键词:
菹草;红心鸭蛋;颗粒饲料;成分
摘要:
测定了菹草粉、菹草颗粒及试验优选的红心蛋颗粒饲料的成分,结果表明:菹草粉的粗蛋白含量为133.6 g/kg,总类胡萝卜素的含量为463.1 mg/kg,两者都大大高于玉米,而粗蛋白与小麦麸相近;菹草颗粒的主要成分含量分别为:红色类胡萝卜素20.59 mg/kg,干物质900.5 g/kg、粗蛋白129.6 g/kg、粗脂肪43.6 g/kg、粗纤维47.5g/kg、无氮浸出物541.6 g/kg、粗灰分138.2g/kg、钙17.2 g/kg、总磷2.4 g/kg.红心蛋颗粒饲料的主要指标符合部颁标准SB/T 10262-1996,生长鸭、产蛋鸭、肉用仔鸭配合饲料的标准.
语种:
中文
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低温脱脂豆粉与大豆豆皮膳食纤维改善馒头品质及老化的比较
作者:
肖安红;何东平;张世宏
期刊:
中国粮油学报 ,2005年20(4):73-76 ISSN:1003-0174
作者机构:
[肖安红; 何东平; 张世宏] 武汉工业学院食品科学与工程学院;武汉工业学院食品科学与工程学院 武汉430031
关键词:
低温脱脂豆粉;大豆豆皮膳食纤维;馒头;品质;老化
摘要:
实验证明适量添加低温脱脂豆粉、大豆豆皮膳食纤维均可制作品质优良的馒头;适量低温脱脂豆粉、大豆豆皮膳食纤维均可作为改良剂提高馒头和面粉的品质、有效地延缓馒头老化;且大豆豆皮膳食纤维较低温脱脂豆粉改良效果好.
语种:
中文
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置换色谱理论研究进展
作者:
张维农
期刊:
武汉轻工大学学报 ,2005年24(1):60-64 ISSN:2095-7386
作者机构:
武汉工业学院,食品科学与工程学院,湖北,武汉,430023
关键词:
色谱技术;研究进展;置换;分离分析;生物样品;综述;重要作用;谱理论;痕量;非线性
摘要:
置换色谱是一种非线性色谱技术,既适合于制备分离又能用于较稀的生物样品或痕量组分的直接分离.因此在生物样品的分离分析领域得到广泛应用.置换色谱理论研究的目的在于揭示置换色谱的本质,对于置换色谱技术的发展起着重要作用.在此对置换色谱理论研究进展进行了综述.
语种:
中文
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