作者机构：
[Miao, Lihong] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Wang, Yue] College of Resources and Environment, Yangtze University, Wuhan 430100, China;Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province, Hangzhou 310058, China;[Mo, Shuhong; Zheng, Xing; Li, Jiake] State Key Laboratory of Eco-Hydraulics in Northwest Arid Region, Xi’an University of Technology, Xi’an 710048, China;Author to whom correspondence should be addressed.
通讯机构：
[Yun Kong] K;Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province, Hangzhou 310058, China<&wdkj&>College of Resources and Environment, Yangtze University, Wuhan 430100, China<&wdkj&>State Key Laboratory of Eco-Hydraulics in Northwest Arid Region, Xi’an University of Technology, Xi’an 710048, China<&wdkj&>Author to whom correspondence should be addressed.
期刊：
FOODBORNE PATHOGENS AND DISEASE,2022年19(1):36-44 ISSN：1535-3141
通讯作者：
Sui, Z.;Wei, Q.
作者机构：
[Wang, Ziquan; Liu, Siyuan; Sui, Zhiwei] Center for Advanced Measurement Science, National Institute of Metrology, No.18, Bei San Huan Dong Lu, Chaoyang District, Beijing, 100029, China;[Zhen, Xiaoxiao] Institute of Chemical Analysis and Biomedicine, Beijing Institute of Metrology, Beijing, China;[Zhou, Guoping] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China;[Wei, Qiang] National Pathogen Resource Center, Chinese Center for Disease Control and Prevention, 155 Changbai Road Changping District, Beijing, 102206, China;[Li, Longquan] Center for Advanced Measurement Science, National Institute of Metrology, No.18, Bei San Huan Dong Lu, Chaoyang District, Beijing, 100029, China, School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Qiang Wei] N;[Zhiwei Sui] C;Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China.<&wdkj&>National Pathogen Resource Center, Chinese Center for Disease Control and Prevention, Beijing, China.
作者机构：
[Chen, Hongbiao; Li, Yun; Cao, Hui; Zhao, Xiuju] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, NDHN, Team Neonatal & Infant Dev Hlth & Nutr, Wuhan 430023, Peoples R China.;[Li, Yun; Yi, Ping] Kindstar Global Precis Med Inst, Wuhan 430223, Peoples R China.;[Wang, Qi] Wuhan Polytech Univ, Sch Food Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Ping Yi; Xiuju Zhao] A;Authors to whom correspondence should be addressed.<&wdkj&>Team of Neonatal & Infant Development, Health and Nutrition, NDHN, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Kindstar Global Precision Medicine Institute, Wuhan 430223, China
摘要：
The number of metabolic syndromes (MetS) is increasing, and a fish phospholipid diet can reduce the risk of MetS. In this study, the changes in lipid metabolism of colon contents were analyzed by extensive lipidomics in mice with metabolic syndrome by fish phospholipid diet, and mice were randomly divided into experimental groups with different diet types by establishing a MetS model. After 14 weeks, the mice were sacrificed and the serum and colon contents were collected. Ultra-high liquid phase tandem mass spectrometry was used for broadly targeted lipidomic analysis, and the qualitative and quantitative detection of lipid metabolism changes in the colonic contents of mice. Under the intervention of fish phospholipids, MetS mice were significantly inhibited, serum total cholesterol (TC) and triglycerides (TG) decreased, serum high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) levels were improved, fasting blood glucose and insulin levels decreased, and inflammatory factors decreased. Through screening, it was found that thirty-three lipid metabolites may be key metabolites and five have significantly changed metabolic pathways. Modularizing lipid metabolites, it is possible to understand the extent to which different types and concentrations of fish phospholipids affect metabolic syndrome. Therefore, our study may provide new therapeutic clues for improving MetS.
作者：
Ye, Shijie;Yin, Dongjie;Sun, Xiaoyan;Chen, Qinyi;Min, Ting;...
期刊：
Molecules,2022年27(23):8374- ISSN：1420-3049
通讯作者：
Limei Wang
作者机构：
[Wang, Hongxun; Chen, Qinyi; Ye, Shijie; Sun, Xiaoyan; Yin, Dongjie; Wang, Limei] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430023, Peoples R China.;[Min, Ting] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Limei Wang] C;College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Trapa bispinosa Roxb. is an economical crop for medicine and food. Its roots, stems, leaves, and pulp have medicinal applications, and its shell is rich in active ingredients and is considered to have a high medicinal value. One of the main functional components of the Trapa bispinosa Roxb. shell is 1-galloyl-beta-D-glucose (beta G), which can be used in medical treatment and is also an essential substrate for synthesizing the anticancer drug beta-penta-o-Galloyl-glucosen (PGG). Furthermore, gallate 1-beta-glucosyltransferase (EC 2.4.1.136) has been found to catalyze gallic acid (GA) and uridine diphosphate glucose (UDPG) to synthesize beta G. In our previous study, significant differences in beta G content were observed in different tissues of Trapa bispinosa Roxb. In this study, Trapa bispinosa Roxb. was used to clone 1500 bp of the UGGT gene, which was named TbUGGT, to encode 499 amino acids. According to the specificity of the endogenous expression of foreign genes in Escherichia coli, the adaptation codon of the cloned original genes was optimized for improved expression. Bioinformatic and phylogenetic tree analyses revealed the high homology of TbUGGT with squalene synthases from other plants. The TbUGGT gene was constructed into a PET-28a expression vector and then transferred into Escherichia coli Transsetta (DE3) for expression. The recombinant protein had a molecular weight of 55 kDa and was detected using SDS-PAGE. The proteins were purified using multiple fermentation cultures to simulate the intracellular environment, and a substrate was added for in vitro reaction. After the enzymatic reaction, the levels of beta G in the product were analyzed using HPLC and LC-MS, indicating the catalytic activity of TbUGGT. The cloning and functional analysis of TbUGGT may lay the foundation for further study on the complete synthesis of beta G in E. coli.
摘要：
以果胶为唯一碳源,从海南采集的土样中筛选出一株能够利用果胶生长并产生果胶酶的菌株,采用3,5-二硝基水杨酸定糖法测定果胶酶酶活,通过生理、生化特性以及16S r RNA基因序列分析比对对菌株进行鉴定,并对其所产果胶酶的酶学性质进行研究。结果表明,分离筛选出一株果胶酶产生菌,编号为YY01,被鉴定为Bacillus niabensis。菌株YY01所产果胶酶的最适p H值为7.0,最适反应温度为45℃;在p H 10.0孵育12 h,仍有52.5%残余酶活力,50℃孵育6 h,仍有64%的残余酶活力。结果显示该酶有较好的耐碱性及较高的热稳定性,具有应用于果汁加工和胡椒脱皮的应用前景。
