作者： 陈天乐

期刊： 甘肃畜牧兽医,2016年46(21):114-116 ISSN：1006-799X

作者机构： 武汉轻工大学动物科学与营养工程学院,湖北 武汉,430023;[陈天乐] 武汉轻工大学

关键词： 果园优质高效土鸡;养殖;新技术

摘要： 现阶段果园土鸡的养殖是一种具有生态性的生态养殖方式,在果园中养鸡不仅可以对果园中闲置空间与资源进行充分的利用,同时也可以将鸡和果树量两种资源进行结合,互相发挥作用,实现互惠互利,进而达到鸡与果实的双丰收.但是在实际土鸡养殖的过程中,依然存在一些关于养殖的问题,并对果园土鸡养殖的效率造成一定的影响.为了解决其中存在的问题,文章中对果园土鸡养殖的新技术进行了探讨,以提升土鸡养殖的效率,并为广大同仁提供养殖方面的信息.

语种： 中文

作者： 蒋加鹏;刘立鹤;吴鹏飞

期刊： 农家致富,2016年(17):36-37 ISSN：1672-6456

作者机构： 武汉轻工大学动科院;标优美生态股份有限公司

关键词： 河蟹养殖;池塘底部;池塘管理;生长空间;生态系统;蓝绿藻;伊乐藻;富营养化;底层水;防洪工作

摘要： <正>俗话说"蟹大小,看水草",高温季节水草养护的好坏,直接决定河蟹养殖的成败。一、池塘管理要点1.控密度水草生长过盛消耗大量水中溶氧,甚至可能造成池塘底部溶氧不足;水草过密也增加水质和底质调节的难度,压缩了河蟹生长空间,同时易造成水体中pH值变化过大,使蟹长期处于相对高的pH值环境。因此,高温季节蟹塘水草覆盖率能控制在50%60%即可,这样有利于构建稳定的生态系统。水体水草过密,应通过

语种： 中文

作者： Xiong, J. L.;Wang, Z. J.;Miao, L. H.;Meng, F. T.;Wu, L. Y.*

期刊： JOURNAL OF ANIMAL AND FEED SCIENCES,2016年25(4):348-353 ISSN：1230-1388

通讯作者： Wu, L. Y.

作者机构： [Meng, F. T.; Wang, Z. J.; Miao, L. H.; Wu, L. Y.; Xiong, J. L.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Wu, L. Y.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Broilers;Candida utilis;Cottonseed meal;Fermentation;Gossypol

摘要： The aim of the study was to determine the effects of solid-state fermentation of cottonseed meal (FCSM) with Candida utilis on broiler growth performance and toxicity in comparison to unfermented cottonseed meal (CSM) or soyabean meal (SBM, control). In total 150 one-day-old male Cobb 400 broilers were randomly assigned to 3 dietary treatments with 5 replications of 10 birds each. Animals were fed control diet with SBM, diet with CSM : SBM (1:1) and diet with FCSM : SBM (1:1) until 21 day of life. Fermentation decreased free gossypol content in CSM from 583.40 to 191.70 mg . kg(-1), and degraded total gossypol from 5830.19 to 3882.91 mg . kg(-1) CSM. In comparison to the control diet, FCSM : SBM (1:1) diet did not affect (P > 0.05) the average daily growth and feed conversion ratio, while CSM : SBM (1:1) diet decreased (P < 0.05) these parameters. However, FCSM : SBM (1:1) diet, similarly to CSM : SBM (1:1) diet, increased (P < 0.05) the relative weight of liver and the serum activity of alanine aminotransferase, and decreased (P < 0.05) the relative weight of thymus in comparison to the control diet. In conclusion, residual gossypol or/and degradation products of gossypol in FCSM may still be hepatotoxic and immunotoxic for broilers when SBM is half-replaced by FCSM in diet (155 g . kg(-1)), although the contents of free gossypol and total gossypol have been markedly reduced and no adverse effect on growth performance was observed.

语种： 英文

作者： 李宝成;王蕾;侯永清;周佳;张越;...

作者机构： [李宝成; 王蕾; 侯永清; 周佳; 张越; 张俊梅] 武汉轻工大学动物营养与饲料科学湖北省重点实验室

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 功能性氨基酸复合物;断奶仔猪;肠道功能

摘要： 功能性氨基酸作为合成多种具有重要功能生物活性分子的必需前体物,参与调节动物的新陈代谢途径,对维持动物正常的生长、发育和繁殖都发挥着至关重要的作用。功能性氨基酸已被证实具有改善肠道结构与功能,促进仔猪生长的作用。本试验旨在研究功能性氨基酸复合物对断奶仔猪肠道功能的影响。试验选取16头健康的21日龄断奶仔猪(杜×长×大),随机分成2个处理组:对照组和复合氨基酸组,每处理组8个重复,每个重复1头猪。对照

语种： 中文

作者： 刘立鹤;蒋加鹏;吴鹏飞

期刊： 渔业致富指南,2016年(19):52-54 ISSN：1008-2840

作者机构： 武汉轻工大学动物科学与营养工程学院;[吴鹏飞] 标优美生态工程股份有限公司;[蒋加鹏; 刘立鹤] 武汉轻工大学

关键词： 蓝藻水华;养殖水体;投饵量;富营养化;强碱性;残饵;防治方法;云斑;植物量;投喂量

摘要： <正>一、蓝藻水华形成的原因高温季节,随着投饵量的不断增多,残饵、粪便、鱼类自身代谢产物的不断进入使得池塘封闭水体富营养化。在强碱性和高氮低磷的养殖水体,更容易出现蓝藻水华。形成蓝藻水华的水体表面往往形成一层绿色的油膜,以云斑状、带状在水面上漂浮,并有难闻的臭味,养殖户称之谓"老绿水"。在生长良好的池塘中浮游植物量一般应在100mg/L以内,而蓝藻塘的"肥水"浮游植物的生物量往

语种： 中文

作者： 罗旋

期刊： 农业工程与装备,2016年43(7):154-155 ISSN：2096-8736

作者机构： 武汉轻工大学 动物科学与营养工程学院,湖北 武汉,430023;[罗旋] 武汉轻工大学

关键词： 稀土元素;动物营养;作用

摘要： 我国稀土资源丰富,已被广泛应用到农业、工业等多个领域.在动物营养中,稀土元素作为重要的饲料添加剂,具有促进动物生长,增强免疫力,降低死亡率等生理作用.笔者在文章中就稀土元素在动物营养中的作用进行综述.

语种： 中文

作者： Ming Li;Zongyi Sun;Chong Wang*;Lin Zhang

期刊： Journal of Parasitic Diseases,2016年40(4):1429-1434 ISSN：0971-7196

通讯作者： Chong Wang

作者机构： [Zongyi Sun] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China;Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan, China;[Chong Wang] Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, China;[Lin Zhang] Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan, China;[Ming Li] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China<&wdkj&>Hubei Collaborative Innovation Centre for Freshwater Aquaculture, Wuhan, China

通讯机构： [Chong Wang] I;Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan, China

关键词： Apiosoma piscicola;Ectocommensal;Ectoparasite;Peritrich;Tachysurus fulvidraco;Ultrastructure

摘要： This paper gave detailed description of the fine structure of Apiosoma piscicola Blanchard 1885. These parasites were collected from the fry of Tachysurus fulvidraco during parasite surveys of fish at Jiangxia Fish Hatchery in Wuhan, Hubei province in May 2011. Its pellicle, peristomial apparatus, nuclei, trochal band and scopula were described, respectively. The relationship between these peritrich ciliates and their hosts was well discussed herein. The scopula of A. piscicola did not adhere directly to the surface of the host epithelial cells, but by means of a secreted adhesive pad of material according to the present study. Besides, no ultrastructural damage to host epithelial cells could be detected in our work. We concluded that these sessilids should be ectocommensals rather than ectoparasites, but not always harmless. In addition, their great variability of body shape and non-strict host specificity were also discussed.

语种： 英文

作者： Rezaei, Reza;Wu, Zhenlong;Hou, Yongqing;Bazer, Fuller W.;Wu, Guoyao*

期刊： 畜牧与生物技术杂志(英文版),2016年7(1):437-458 ISSN：1674-9782

通讯作者： Wu, Guoyao

作者机构： [Bazer, Fuller W.; Wu, Guoyao; Rezaei, Reza] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Zhenlong; Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Hou, Yongqing; Wu, Guoyao] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

通讯机构： [Wu, Guoyao] T;[Wu, Guoyao] C;[Wu, Guoyao] W;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.

关键词： Development;Health;Livestock;Mammary gland;Milk;Neonates;Production;Sows

摘要： Milk is synthesized by mammary epithelial cells of lactating mammals. The synthetic capacity of the mammary gland depends largely on the number and efficiency of functional mammary epithelial cells. Structural development of the mammary gland occurs during fetal growth, prepubertal and post-pubertal periods, pregnancy, and lactation under the control of various hormones (particularly estrogen, growth hormone, insulin-like growth factor-I, progesterone, placental lactogen, and prolactin) in a species- and stage-dependent manner. Milk is essential for the growth, development, and health of neonates. Amino acids (AA), present in both free and peptide-bound forms, are the most abundant organic nutrients in the milk of farm animals. Uptake of AA from the arterial blood of the lactating dam is the ultimate source of proteins (primarily β-casein and α-lactalbumin) and bioactive nitrogenous metabolites in milk. Results of recent studies indicate extensive catabolism of branched-chain AA (leucine, isoleucine and valine) and arginine to synthesize glutamate, glutamine, alanine, aspartate, asparagine, proline, and polyamines. The formation of polypeptides from AA is regulated not only by hormones (e.g., prolactin, insulin and glucocorticoids) and the rate of blood flow across the lactating mammary gland, but also by concentrations of AA, lipids, glucose, vitamins and minerals in the maternal plasma, as well as the activation of the mechanistic (mammalian) target rapamycin signaling by certain AA (e.g., arginine, branched-chain AA, and glutamine). Knowledge of AA utilization (including metabolism) by mammary epithelial cells will enhance our fundamental understanding of lactation biology and has important implications for improving the efficiency of livestock production worldwide.

语种： 英文

作者： 王树辉;涂治骁;王海波;陈少魁;朱惠玲;...

作者机构： [王树辉; 涂治骁; 王海波; 陈少魁; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 仔猪;脂多糖;亚麻油;肠道损伤

摘要： 本试验研究了饲粮添加5%亚麻油对脂多糖诱导仔猪肠道损伤的调控作用,并从组织脂肪酸组成、形态学结构、组织功能指标以及抗炎的核苷酸结合寡聚域受体(NOD)和Toll样受体4(TLR4)信号通路基因的表达等方面探讨其作用机理。选择24头体重为(9.15±0.8)kg的杜(长(大三元杂交断奶仔猪,随机分为4个处理。本试验采用2×2因子设计,两个因子分别为饲粮处理和应激处理,得到以下四个分组:1)玉米油+生

语种： 中文

作者： 王磊;周佳;吕阳;张越;李雪妮;...

作者机构： [王磊; 周佳; 吕阳; 张越; 李雪妮; 吴涛] 武汉轻工大学动物营养与饲料科学湖北省重点实验室

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

会议论文集名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集

关键词： 大肠杆菌;大肠杆菌耐热肠毒素;仔猪;腹泻模型

摘要： 大肠杆菌是导致仔猪腹泻的主要致病菌之一,耐热肠毒素A(STa)是其毒性最强的致病因子。为更好研究STa导致腹泻的机理,本试验尝试建立单一肠毒素STa作用下的仔猪腹泻模型。试验选取7日龄左右健康且大小匀称的杜×长×大三元杂交哺乳仔猪24头(未做大肠杆菌相关疾病免疫),按单因子设计随机分为4个组,每组设置6个重复,每个重复1头仔猪。4个处理组分别为空白对照组、大肠杆菌LMG194-pBAD-STa组(

语种： 中文

作者： 陈少魁;刘玉兰;王海波;王秀英;朱惠玲;...

期刊： 动物营养学报,2016年28(3):891-898 ISSN：1006-267X

作者机构： [陈少魁; 刘玉兰; 王海波; 王秀英; 朱惠玲; 张晶; 王树辉; 涂治骁] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023

关键词： 仔猪;亚麻籽油;脂多糖;肝脏

摘要： 本试验旨在研究亚麻籽油对脂多糖(LPS)刺激仔猪肝脏Toll样受体4(TLR4)和核苷酸结合寡聚化结构域(NOD)信号通路关键基因表达的影响。选取24头断奶仔猪,按体重相近原则随机分为4个组,分别为对照组、LPS组、2.5%亚麻籽油组(2.5%亚麻籽油+LPS)、5.0%亚麻籽油组(5.0%亚麻籽油+LPS),每组6个重复,每个重复1头猪,试验期21 d。试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后屠宰仔猪,取肝脏,测定TLR4和NOD信号通路关键基因及相关炎性介质的mRNA表达水平。结果表明:1)LPS刺激显著提高了肝脏肿瘤坏死因子-α(TNF-α)、环氧酶2(COX2)、热休克蛋白70(HSP70)的mRNA相对表达量(P<0.05),2.5%亚麻籽油可显著降低COX2、TNF-α的mRNA相对表达量(P<0.05),5.0%亚麻籽油可显著降低TNF-α的mRNA相对表达量(P<0.05)。2)LPS刺激显著提高了肝脏TLR4、髓样分化因子88(MyD88)、白细胞介素-1受体相关激酶1(IRAK1)、NOD1、NOD2、受体互作蛋白2(RIPK2)、核因子-κB(NF-κB)的mRNA相对表达量(P<0.05);2.5%亚麻籽油可显著降低NOD1、NOD2的mRNA相对表达量(P<0.05),有降低RIPK2 mRNA相对表达量的趋势(0.05≤P<0.10);5.0%亚麻籽油可显著降低NOD2的mRNA相对表达量(P<0.05)。这表明LPS刺激导致仔猪发生炎症反应,亚麻籽油可能通过抑制NOD信号通路进而缓解肝脏炎症反应。

语种： 中文

作者： Wang, Chong;Liang, Yin-Quan*;Li, Ming*;Zhang, Yu;Shen, Zai-Jun;...

期刊： Mitochondrial DNA Part A,2016年27(6):4617-4619 ISSN：2470-1394

通讯作者： Liang, Yin-Quan;Li, Ming

作者机构： [Liang, Yin-Quan; Wang, Chong] Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan, Peoples R China.;[Liang, Yin-Quan; Wang, Chong] Chinese Acad Sci, Wuhan, Peoples R China.;[Li, Ming] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Zhang, Yu; Shen, Zai-Jun; Jiang, Zhi-Wu] Jin Sha River Ludila Hydropower Co, Kunming, Peoples R China.

通讯机构： [Liang, Yin-Quan] M;[Liang, Yin-Quan] C;[Li, Ming] W;Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan, Peoples R China.;Chinese Acad Sci, Wuhan, Peoples R China.

关键词： *Cobitida;*Complete mitochondrial genome;*Cypriniformes;*Triplophysa venusta

摘要： The complete mitochondrial DNA sequence of Triplophysa venusta was determined using a PCR-based method in our present study. The genome is totally 16 574 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA, 22 transfer RNA genes, and 1 non-coding control region. The overall base composition of T. venusta is A 27.8%, T 26.9%, C 26.9%, and G 18.4%, with an A + T bias of 54.7%. This complete mitogenome of T. venusta provides a basic data for studies on species identification, molecular systematics, and conservation genetics. The phylogenetic trees consistently showed that the Family Cobitidae was divided into three major clades: the subfamily Botiidae, the subfamily Nemaeheilidae, and the subfamily Cobitinae. Cobitinae and Nomaeheilidae formed a main Loach clade, with Botiidae clustered at the basal. © 2015 Taylor & Francis.

语种： 英文

作者： Ye, Chun;Li, Sali;Yao, Wenxu;Xu, Lei;Qiu, Yinsheng*;...

期刊： INNATE IMMUNITY,2016年22(3):196-204 ISSN：1753-4259

通讯作者： Qiu, Yinsheng

作者机构： [Liu, Yu; Hou, Yongqing; Ye, Chun; Xu, Lei; Li, Sali; Qiu, Yinsheng; Wu, Zhongyuan; Yao, Wenxu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.

通讯机构： [Qiu, Yinsheng] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

关键词： Baicalin;anti-inflammation;NLRP3 inflammasome;piglet peripheral blood mononuclear cell;reactive oxygen species

摘要： In this study, the anti-inflammatory effects and mechanisms of baicalin on LPS-induced NLRP3 inflammatory pathway were investigated in piglet mononuclear phagocytes (control, LPS stimulation, LPS stimulation + 12.5 μg/ml baicalin, LPS stimulation + 25 μg/ml baicalin, LPS stimulation + 50 μg/ml baicalin and LPS stimulation + 100 μg/ml baicalin). The levels of reactive oxygen species (ROS), the secretion levels of IL-1β, IL-18 and TNF-α, mRNA expression levels of IL-1β, IL-18, TNF-α and NLRP3, as well as the protein levels of cleaved caspase-1 p20 were significantly increased after LPS-challenge in vitro. However, LPS stimulation did not influence apoptosis-associated speck-like protein and caspase-1 mRNA levels, which are also components of the NLRP3 inflammasome. Baicalin at 50 μg/ml and 100 μg/ml could inhibit the production of ROS, TNF-α, IL-1β and IL-18, and down-regulate mRNA expression of IL-1β, IL-18, TNF-α and NLRP3, as well as expression of cleaved caspase-1 p20. These results showed that the anti-inflammatory effects of baicalin occurred via the regulation of the release of ROS and mRNA expression of NLRP3. The anti-inflammatory activity of baicalin could be related to the suppression of NLRP3 inflammasome pathway under LPS stimulation. © 2016 The Author(s).

语种： 英文

作者： 张越;侯永清;王蕾;李鹏;徐海旺

作者机构： [张越; 侯永清; 王蕾; 李鹏; 徐海旺] 武汉轻工大学动物营养与饲料科学湖北省重点实验室

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 凝结芽孢杆菌;断奶仔猪;生长性能;血液指标;肠道结构

摘要： 益生菌可抑制机体中有害菌生长,促进优势菌群增殖,增强非特异性免疫功能,从而促进动物生长、增强机体免疫力、提高饲料转化率,产生与抗生素相似的作用效果。本试验首先通过牛津杯法比较了凝结芽孢胞杆菌、地衣芽孢杆菌、屎肠球菌、干酪乳杆菌对大肠杆菌K88的抑菌作用,发现凝结芽孢杆菌较其他三株菌抑菌能力更好。然后以断奶仔猪为研究对象,研究了日粮中添加凝结芽孢杆菌对断奶仔猪生长性能、血液生化指标及肠道形态结构的影

语种： 中文

作者： 涂治骁;陈少魁;王海波;王树辉;朱惠玲;...

作者机构： [涂治骁; 陈少魁; 王海波; 王树辉; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 仔猪;中链脂肪酸;肝脏损伤

摘要： 本文通过测定肝脏脂肪酸组成、形态结构与功能、核苷酸结合寡聚域受体(NOD)和Toll样受体4(TLR4)信号通路及其下游相关基因的表达,探讨了中链脂肪酸对大肠杆菌脂多糖(LPS)诱导的断奶仔猪肝脏损伤的调控及其机制。试验共选用24头杜×长×大断奶仔猪[体重(9.0±0.7)kg],分为4个组。试验采用2×2因子设计,两个主因子分别:1)饲粮处理(玉米油或中链脂肪酸);2)免疫应激处理(0.9%Na

语种： 中文

作者： 李华伟;王宗俊;祝倩;孔祥峰;印遇龙;...

期刊： 天然产物研究与开发,2016年28(10):1534-1539 ISSN：1001-6880

作者机构： 武汉轻工大学动物科学与营养工程学院, 中国科学院亚热带农业生态过程重点实验室;[李华伟; 祝倩; 孔祥峰; 印遇龙] 湖南省畜禽健康养殖工程技术研究中心;[李华伟] 农业部中南动物营养与饲料科学观测实验站, 武汉, 430023;武汉轻工大学动物科学与营养工程学院, 武汉, 430023;中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室

关键词： 妊娠母猪;繁殖性能;哺乳仔猪;生长性能;发酵中药渣

摘要： 为了探讨中药渣在母猪饲粮中添加的可行性,试验选用2~4胎次、预产期相近的健康二元母猪60头,随机分为对照组、中药渣组和发酵中药渣组,比较研究发酵前和发酵后中药渣对母猪繁殖性能和仔猪发育的影响。结果表明:与对照组相比,发酵中药渣组产仔数、产活仔数和断奶平均日增重均有所增加,断奶窝增重和哺乳仔猪腹泻率显著增加,母猪背膘损失有所降低;中药渣组木乃伊数略有增加,仔猪腹泻率明显增加。由上可见,饲粮中添加发酵中药渣制剂虽然在短期内造成仔猪一定程度的腹泻,但可提高母猪繁殖性能和仔猪生长性能。

语种： 中文

作者： Yi, Dan;Hou, Yongqing*;Wang, Lei;Long, Minhui;Hu, Shengdi;...

期刊： Amino Acids,2016年48(2):523-533 ISSN：0939-4451

通讯作者： Hou, Yongqing

作者机构： [Hu, Chien-An Andy; Long, Minhui; Mei, Huimin; Hou, Yongqing; Yan, Liqiong; Wang, Lei; Wu, Guoyao; Yi, Dan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Hu, Shengdi; Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

关键词： N-acetylcysteine;Intestinal cells;Protein synthesis;Glutathione

摘要： Dietary supplementation with N-acetylcysteine (NAC) has been reported to improve intestinal health and treat gastrointestinal diseases. However, the underlying mechanisms are not fully understood. According to previous reports, NAC was thought to exert its effect through glutathione synthesis. This study tested the hypothesis that NAC enhances enterocyte growth and protein synthesis independently of cellular glutathione synthesis. Intestinal porcine epithelial cells were cultured for 3 days in Dulbecco's modified Eagle medium containing 0 or 100 μM NAC. To determine a possible role for GSH (the reduced form of glutathione) in mediating the effect of NAC on cell growth and protein synthesis, additional experiments were conducted using culture medium containing 100 μM GSH, 100 μM GSH ethyl ester (GSHee), diethylmaleate (a GSH-depletion agent; 10 μM), or a GSH-synthesis inhibitor (buthionine sulfoximine, BSO; 20 μM). NAC increased cell proliferation, GSH concentration, and protein synthesis, while inhibiting proteolysis. GSHee enhanced cell proliferation and GSH concentration without affecting protein synthesis but inhibited proteolysis. Conversely, BSO or diethylmaleate reduced cell proliferation and GSH concentration without affecting protein synthesis, while promoting protein degradation. At the signaling level, NAC augmented the protein abundance of total mTOR, phosphorylated mTOR, and phosphorylated 70S6 kinase as well as mRNA levels for mTOR and p70S6 kinase in IPEC-1 cells. Collectively, these results indicate that NAC upregulates expression of mTOR signaling proteins to stimulate protein synthesis in enterocytes independently of GSH generation. Our findings provide a hitherto unrecognized biochemical mechanism for beneficial effects of NAC in intestinal cells. © 2015 Springer-Verlag Wien.

语种： 英文

作者： Wang, H.;Hou, Y.;Guo, J.;Chen, H.;Liu, X.;...

期刊： ANIMAL GENETICS,2016年47(1):49-61 ISSN：0268-9146

通讯作者： Zhu, M.

作者机构： [Zhu, M.; Zhao, S.; Hou, Y.; Liu, X.; Wang, H.; Guo, J.] Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China.;[Wang, H.] Huazhong Agr Univ, Coll Informat, Wuhan 430070, Peoples R China.;[Chen, H.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Z.] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China.;[Zhu, M.; Zhao, S.] Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Peoples R China.

通讯机构： [Zhu, M.] H;Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China.

关键词： differentially expressed gene;lymphocyte count;pig;poly I:C;transcriptome

摘要： Lymphocyte count is an important phenotypic metric that has been reported to be related to the individual antiviral capacity of pigs and other mammals. To date, aside from information regarding several genes and pathways, little is known about the mechanism by which gene expression affects variation in lymphocyte count. In this work, we investigated the lymphocyte count variation after poly I:C stimulation and compared the transcriptomes of pigs with large and small differences of lymphocyte counts before and after poly I:C stimulation. Pigs with large and small differences of lymphocyte counts were designated as extreme response (ER) and moderate response (MR) pigs respectively. Lymphocyte counts in all animals were observed to decline after poly I:C stimulation. Transcriptomic analysis identified 1121 transcripts (981 differentially expressed genes) in MR pigs and 1045 transcripts (904 differentially expressed genes) in ER pigs. We found that the majority of the differentially expressed genes were involved in both innate and adaptive immune responses. However, the innate immune response of ER pigs was more rapid than that of MR pigs. Results indicated that the activation of signaling pathways associated with cell death, cytotoxicity and apoptosis may contribute to the poly I:C-induced decrease of lymphocyte counts in the periphery. Moreover, the differential expression patterns of chemokines and FAS either totally or partially provided an interpretation for the different degrees of decrease in the lymphocyte counts between MR and ER pigs. Overall, our study will provide further understanding of the molecular basis for the antiviral capacity of pigs and other mammals. © 2015 Stichting International Foundation for Animal Genetics.

语种： 英文

作者： 刘世操;王春维;卜小丽;韩朋伟;陈帆;...

作者机构： [刘世操; 王春维; 卜小丽; 韩朋伟; 陈帆; 祝爱侠] 武汉轻工大学动物营养与饲料安全湖北省协同创新中心;[刘世操; 王春维; 卜小丽; 韩朋伟; 陈帆; 祝爱侠] 武汉轻工大学畜禽饲料工程技术研究中心

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 肉仔鸡;电气石;重金属;生长性能;血液指标

摘要： 本试验旨在研究电气石对肉仔鸡饲料中重金属的脱毒效果。选用240只1日龄健康科宝500肉鸡,按照体重相近原则随机分为5个处理组,每个处理组4个重复,每个重复12只鸡,分别为空白对照组(基础饲粮)和重金属污染组(基础饲粮+10 mg/kg铅+0.3 mg/kg汞+5 mg/kg镉)、试验1组、试验2组、试验3组。试验1组、试验2组、试验3组日粮分别在重金属日粮基础上添加500、1 000、1 500

语种： 中文

作者： Dong, Z. L.;Wang, Y. W.;Song, D.;Hou, Y. J.;Wang, W. W.;...

期刊： Animal Feed Science and Technology,2016年212:42-51 ISSN：0377-8401

通讯作者： Li, A. K.

作者机构： [Dong, Z. L.] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.;[Wang, W. W.; Li, A. K.; Qi, W. T.; Yun, T. T.; Song, D.; Hou, Y. J.; Wang, Y. W.] Acad Sci & Technol, State Adm Grain, Beijing 100037, Peoples R China.

通讯机构： [Li, A. K.] A;Acad Sci & Technol, State Adm Grain, Beijing 100037, Peoples R China.

关键词： Broiler;Extract of Camellia oleifera seed;Growth performance;Immune function;Intestinal mucosal;Pre-microencapsulated Enterococcus fecalis

摘要： The objective of this study was to investigate the effects of microencapsulated Enterococcus fecalis (MEF) and the extract of Camellia oleifera seed (ECOS) on growth performance, morphological parameters and gene expressions related to barrier functions of the intestinal mucosa in broiler chickens. 160-day-old Arbor Acres male broilers were randomly allotted to 4 treatments with 8 replicates of 5 chicks in a 2. ×. 2 factorial arrangement, evaluating two levels of MEF (0 and 10. g/kg), and two levels of ECOS (0 and 300. mg/kg). The results showed that there was no significant interaction effect of MEF and ECOS on growth performance, morphometric parameters of jejunum and ileum, and mRNA expression of MUC2, occludin and claudin-1 in jejunum and ileum mucosa. Compared with other groups, diet supplemented with MEF significantly improved FCR from days 21 to 42, and significantly improved ADG from days 0 to 42. Dietary MEF significantly increased villus height and villus height to crypt depth ratio in jejunum and ileum, the concentrations of sIgA, IL-2, IL-6, TNF-α, and significantly up-regulated the gene expression of sIgA, MUC2, occludin and claudin-1 in jejunum and ileum mucosa. The interaction effect of MEF and ECOS was significant on the concentrations of sIgA, IL-2, IL-6, TNF-α in jejunum mucosa on day 42 and on the concentrations of sIgA, IL-2, TNF-α in ileum mucosa on day 42. These results indicated that MEF can improve growth performance and enhance intestinal barrier functions by improving its morphological development, increasing cytokine level, and up-regulating the gene expressions of sIgA, MUC2, occludin and claudin-1. But ECOS had slightly beneficial effects on growth performance and intestinal barrier functions. © 2015.

语种： 英文