Biomimetic dynamic membrane (BDM): Fabrication method and roles of carriers and laccase
作者：
Zhu, Zhenzhou;Chen, Zhe;Luo, Xiao;Liang, Wenzhong;Li, Shuyi;...
期刊：
Chemosphere ,2020年240(Feb.):124882 ISSN：0045-6535
通讯作者：
Zhang, Wenxiang
作者机构：
[Li, Shuyi; Zhu, Zhenzhou; Luo, Xiao; Chen, Zhe; Zhang, Wenxiang; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Liang, Wenzhong] Minist Environm Protect, South China Inst Environm Sci, Guangzhou 510655, Guangdong, Peoples R China.;[Zhang, Wenxiang] Guangdong Univ Technol, Inst Environm & Ecol Engn, Sch Environm Sci & Engn, Guangzhou 510006, Guangdong, Peoples R China.;[Zhang, Wenxiang; Hao, Tianwei] Univ Macau, Fac Sci & Technol, Dept Civil & Environm Engn, Macau, Peoples R China.;[Yang, Zi] Ohio State Univ, Dept Mat Sci & Engn, Columbus, OH 43210 USA.
通讯机构：
[Zhang, Wenxiang] U;Univ Macau, Fac Sci & Technol, Dept Civil & Environm Engn, Macau, Peoples R China.
关键词：
Biomimetic dynamic membrane (BDM);Carrier adsorption;Fabrication method;Laccase activity;Laccase immobilization mechanism
摘要：
Biomimetic dynamic membrane (BDM) has been" employed as a promising membrane separation technology regarding water/wastewater treatment (Model pollutant is methylene blue). Given its catalytic function on micro-pollutant removal and fouling control, detailed mechanism for impacts of fabrication method, carriers (CNT and GO) and laccase on the construction of biomimetic layer and enzyme immobilization have not been clear so far. In this work, the BDM performance with various fabrication methods, carriers and laccase were investigated and verified. The BDM fabrication tests demonstrated that BDM with mixed filtration method had better filtration performance (up to 120 L m(-2) h(-1) flux and 80% removal rate) than BDM with stepwise filtration method. Moreover, the laccases immobilized on GO exhibited a stronger laccase activity than those on CNT. Increasing CNT or GO dosage strengthened removal rate, but lowered flux, meanwhile flux and removal rate exhibited a significant fluctuation with certain laccase dosage. At 25 g m(-2) CNT or GO dosage and 50 g m(-2) laccase dosage, the optimized flux and removal rate values were obtained. Further study investigated the surface morphology and property of BDM, showing that BDM with mixed filtration method turned out to be the optimized enzyme immobilization mechanism and fabrication method. In addition, during multiple filtration cycles, with the optimized conditions, the removal rate, flux and laccase activity of BDM could maintain at high levels. On account of the finding of the present study, selecting a suitable fabrication method, appropriate CNT or GO dosage and laccase dosage can indeed optimize the structure of biomimetic layer and enzyme immobilization, expanding its possibility on sustainable operation. (C) 2019 Elsevier Ltd. All rights reserved.
语种：
英文
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Recent advances in biotransformation of saponins
作者：
He, Yi;Hu, Zhuoyu;Li, Aoran;Zhu, Zhenzhou;Yang, Ning;...
期刊：
Molecules ,2019年24(13):2365 ISSN：1420-3049
通讯作者：
He, Jingren;Cheng, Shuiyuan;Wang, Chengtao
作者机构：
[Ying, Zixuan; Cheng, Shuiyuan; He, Yi; Li, Aoran; Zhu, Zhenzhou; Hu, Zhuoyu; He, Jingren; Cheng, SY; Yang, Ning] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Hubei, Peoples R China.;[He, Yi; Zhu, Zhenzhou; He, Jingren] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[He, Yi; Zhu, Zhenzhou; He, Jingren] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Wang, Chengtao; Yin, Sheng] BTBU, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.
通讯机构：
[He, JR; Cheng, SY; He, Jingren] W;[He, Jingren] M;[Wang, Chengtao] B;Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Hubei, Peoples R China.;Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.
关键词：
saponin;biotransformation;chemical structure;chemistry;human;metabolism;Biotransformation;Humans;Metabolic Networks and Pathways;Molecular Structure;Saponins
摘要：
Saponins are a class of glycosides whose aglycones can be either triterpenes or helical spirostanes. It is commonly recognized that these active ingredients are widely found in various kinds of advanced plants. Rare saponins, a special type of the saponins class, are able to enhance bidirectional immune regulation and memory, and have anti-lipid oxidation, anticancer, and antifatigue capabilities, but they are infrequent in nature. Moreover, the in vivo absorption rate of saponins is exceedingly low, which restricts their functions. Under such circumstances, the biotransformation of these ingredients from normal saponins-which are not be easily adsorbed by human bodies-is preferred nowadays. This process has multiple advantages, including strong specificity, mild conditions, and fewer byproducts. In this paper, the biotransformation of natural saponins-such as ginsenoside, gypenoside, glycyrrhizin, saikosaponin, dioscin, timosaponin, astragaloside and ardipusilloside-through microorganisms (Aspergillus sp., lactic acid bacteria, bacilli, and intestinal microbes) will be reviewed and prospected. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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银杏大麦酒的酿造工艺及品质分析
作者：
李傲然;胡倬瑜;杨宁;孙郡;何静仁;...
期刊：
中国酿造 ,2019年38(1):193-199 ISSN：0254-5071
作者机构：
武汉轻工大学食品科学与工程学院湖北省农产品加工与转化重点实验室,大宗粮油精深加工省部共建教育部重点实验室,湖北武汉430023;[胡倬瑜; 何毅; 杨宁; 孙郡; 何静仁; 李傲然] 大宗粮油精深加工省部共建教育部重点实验室
关键词：
银杏大麦酒;酿造工艺;抗氧化性;响应面试验
摘要：
以银杏果和大麦为原料,研究银杏大麦酒的酿造工艺,通过比较不同品牌酵母产酒精能力的大小选择广西丹宝利酵母作为银杏大麦酒的发酵菌。研究了不同的酵母菌接种量、发酵温度、初始pH值、初始糖度对银杏大麦酒酒精含量的影响;在此基础上进行了4因素3水平银杏大麦酒发酵工艺条件的响应面试验优化。结果表明,银杏大麦酒发酵的最佳工艺条件为:初始糖度22.80°Bx、接种量0.24%、初始pH值3.70、发酵温度27.0℃。在此优化条件下,银杏大麦酒的酒精度为11.46%vol,测定其在最佳酿造过程中总酸、总酚、总黄酮含量及抗氧化性能力。结果表明,银杏大麦酒中总酸、总酚以及总黄酮含量最高分别为121 mg/100 m L、0.34 mg/m L、1.6 mg/m L,且具有良好的抗氧化活性。
语种：
中文
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Modulation of lipid metabolism and colonic microbial diversity of high-fat-diet C57BL/6 mice by inulin with different chain lengths
作者：
Zhu, Zhenzhou;Huang, Yuqi;Luo, Xiao;Wu, Qian;He, Jingren;...
期刊：
Food Research International ,2019年123:355-363 ISSN：0963-9969
通讯作者：
Li, Shuyi;Barba, Francisco J.
作者机构：
[Huang, Yuqi; Li, Shuyi; Zhu, Zhenzhou; Luo, Xiao; Wu, Qian; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Li, Shuyi; Zhu, Zhenzhou; He, Jingren] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Barba, Francisco J.] Univ Valencia, Fac Pharm, Prevent Med & Publ Hlth Food Sci Toxicol & Forens, Nutr & Food Sci Area, Avda Vicent Andres Estelles S-N, E-46100 Valencia, Spain.;[Barba, Francisco J.] Univ Valencia, Fac Pharm, Avda Vicent Andres Estelles S-N, E-46100 Valencia, Spain.
通讯机构：
[Li, Shuyi] W;[Barba, Francisco J.] U;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Univ Valencia, Fac Pharm, Avda Vicent Andres Estelles S-N, E-46100 Valencia, Spain.
关键词：
Chain length;High fat diet;Inulin;Lipid metabolism;Microbial diversity;Modulation
摘要：
The physicochemical properties, biological functions and microbial degradation of inulins differ according to their degree of polymerization. However, the relationship between inulin activities and its effect on gut microbiota remains unknown. In this study, high fat diet with inulin (1 or 5 g/kg·bw), either with short or long chains groups were administered to different groups of mice (n = 10) for 10 weeks in order to investigate the effect of inulin on the microbial diversity of the animals. Litchi pericarp procyanidins (LPPC) were used for comparison purposes. Furthermore, the lipid metabolism and key regulator genes in mice were determined. The results indicated that natural inulin (1 g/kg·bw) ingestion reduced the body weight of fat mice between week 6–9. Glutathione peroxidase (GSH-Px) activity in liver was remarkably higher after adding long chain inulin (5 g/kg·bw) compared to high-fat-diet mice. Moreover, high dose of natural inulin regulated malondialdehyde and advanced glycation end-products levels in mice liver. Likewise, the high dose of short-chain inulin increased sterol response element binding protein 1 (SREBP-1), β-Hydroxy β-methylglutaryl-CoA (HMG-CoA) and ATP-binding cassette transporter A1 (ABCA1) genetic expression. A significant change on the abundance of six genera in gut microbial profile suggested that inulin has the ability to modulate the lipid metabolism regardless of chain length, mainly due to its impact on colon microbiota variety. © 2019
语种：
英文
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Transport of Flavanolic Monomers and Procyanidin Dimer A2 across Human Adenocarcinoma Stomach Cells (MKN-28)
作者：
Li, Shuyi;Li, Junsheng;Sun, Yiwei;Huang, Yuqi;He, Jingren;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2019年67(12):3354-3362 ISSN：0021-8561
通讯作者：
Zhu, Zhenzhou
作者机构：
[Li, Shuyi; Li, Junsheng; Sun, Yiwei; Huang, Yuqi; He, Jingren; Zhu, Zhenzhou] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China;[Li, Shuyi; He, Jingren; Zhu, Zhenzhou] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China
通讯机构：
[Zhu, Zhenzhou] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China. Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.
关键词：
Biochemistry;Cell culture;Cells;Dimers;Efficiency;High performance liquid chromatography;Mass spectrometry;Monomers;Epicatechin;LSCM;Procyanidin a2;stomach barrier;Transport efficiency;Flavonoids;biflavonoid;catechin;flavanone;flavanone derivative;plant extract;proanthocyanidin;procyanidin;adenocarcinoma;bioavailability;chemistry;dimerization;fruit;high performance liquid chromatography;human;lychee;metabolism;stomach mucosa;transport at the cellular level;tumor cell line;Adenocarcinoma;Biflavonoids;Biological Availability;Biological Transport;Catechin;Cell Line, Tumor;Chromatography, High Pressure Liquid;Dimerization;Flavanones;Fruit;Gastric Mucosa;Humans;Litchi;Plant Extracts;Proanthocyanidins
摘要：
It has been proven that A-type procyanidins, containing an additional ether bond, compared to B-type procyanidins are also bioavailable in vitro and in vivo. However, their bioavailability and absorption in the gastrointestinal tract remain uncertain. In this study, a model of the human adenocarcinoma stomach cell line (MKN-28) was established to explore the cellular transport of flavanolic monomers and procyanidin dimer A2, which were isolated from the litchi pericarp extract. After the integrity and permeability of the cell monolayer were ensured by measurement of the transepithelial electrical resistance and the apparent permeability coefficient for Lucifer yellow, the transportation of procyanidins A2 and B2, (-)-epicatechin (EC), and (+)-catechin (CC) was studied at pH 3.0, 5.0, or 7.0 in the apical side, with compound concentrations of 0.05 and 0.1 mg/mL based on the cytotoxicity test. High-performance liquid chromatography and liquid chromatography-mass spectrometry analyses indicated that EC, CC, and A2 were transported in the MKN-28 cell line from 30 to 180 min, while B2 showed no transport. The maximal transport efficiencies of EC, CC, and A2 were 23 ± 0.81, 13.16 ± 1.53, and 16.41 ± 1.36%, respectively, existing at 120, 180, and 120 min of transportation. Laser scanning confocal microscopy analysis presented the dynamic transmission of EC, in accordance with the result of concentration determination, suggesting that the A-type procyanidins are possibly absorbed through the stomach barrier, which is pH- and time-dependent. © 2019 American Chemical Society.
语种：
英文
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Molecular characteristics of kappa-selenocarrageenan and application in green synthesis of silver nanoparticles
作者：
Jin, Weiping* ;Yu, Yiran;Hou, Wenfu;Wang, Guozhen;Zhu, Zhenzhou;...
期刊：
International Journal of Biological Macromolecules ,2019年141:529-537 ISSN：0141-8130
通讯作者：
Jin, Weiping
作者机构：
[Cheng, Shuiyuan; Wang, Guozhen; Zhu, Zhenzhou; Yu, Yiran; Jin, Weiping; Hou, Wenfu; Huang, Qingrong; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Wuhan 430023, Peoples R China.;[Huang, Qingrong] Rutgers State Univ, Sch Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA.
通讯机构：
[Jin, Weiping] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Wuhan 430023, Peoples R China.
关键词：
Anti-microbial activity;Kappa-selenocarrageenan;Molecular structure;Silver nanoparticles;kappa-Carrageenan
摘要：
Selenium is an essential trace element in human body, and kappa-selenocarrageenan (Se-car) is an organic source of selenium supplement. To further utilize Se-car in food packaging, biotherapy or biosensor, the molecular information of Se-car was characterized here and multi-functional Ag NPs synthesized by Se-car were fabricated. Results of GPC-MALLS, FTIR, potentiometric titration, and intrinsic viscosity showed that Se-car was polymerized by nearly 22 basic units of disaccharide. Sixty-four percentage of sulfated groups (SO42-) in carrageenan was replaced by selenium acid (SeO32-), which belonged to weak acid resulting from a gradually decrease of zeta-potential with acidity process to pH 1.0. Besides, the capacity of biosynthesis silver nanoparticles (Ag NPs) by Se-car was studied and it made a comparison with kappa-carrageenan. Results exhibited that Se-car could serve as an efficient reducing and capping agent for Ag NPs fabrication (remarked as Se-car@Ag). The k(app) of Se-car@Ag NPs for catalyzing 4-NP degradation was 2.14 x 10(-2) s(-1). Antibacterial test revealed Se-car@Ag had an ability to inhibit the growth of Escherichia coli and Staphylococcus aureus. To combine the selenium health benefit and functional metal nanoparticles, Se-car@Ag might have potential applications in multiple areas like medicine, disease diagnostic, and drug delivery. (C) 2019 Elsevier B.V. All rights reserved.
语种：
英文
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紫薯蛋白-葡萄糖美拉德产物制备及其与花色苷相互作用研究
作者：
岁鲁姗;詹少颖;李佳;安孝宇;何静仁;...
期刊：
食品工业科技 ,2019年40(19):49-54,60 ISSN：1002-0306
作者机构：
武汉轻工大学食品科学与工程学院,湖北武汉430023;湖北省农业科学院粮食作物研究所,湖北武汉430064;[安孝宇; 祝振洲; 杨宁; 金伟平; 何静仁; 李佳; 詹少颖; 岁鲁姗] 武汉轻工大学
关键词：
紫薯蛋白;美拉德反应;花色苷;相互作用
摘要：
通过干法美拉德反应制备紫薯蛋白-葡萄糖美拉德产物,并分析其与花色苷相互作用.采用聚丙烯酰胺凝胶电泳(SDS-PAGE)测定产物的分子质量,运用傅里叶红外变换光谱(FIRT)、差示扫描量热(DSC)和荧光光谱等多种方法对产物进行结构表征.结果 表明:美拉德反应主要产物分子量为25 kDa;相较于紫薯蛋白花色苷复合物,美拉德产物的红外吸收强度普遍较低,尤其体现于酰胺Ⅰ带处的红外吸收,美拉德产物变性峰温度(76℃)较高,变性焓值(366.7 J/g)较低;美拉德产物的最大荧光发射波长为340 nm,花色苷猝灭美拉德产物荧光为动静态联合猝灭,静态猝灭为主导;花色苷与美拉德产物之间的相互作用力属于强作用力,包括范德华力和氢键.花色苷与紫薯蛋白-葡萄糖美拉德产物相互作用结合位点靠近色氨酸残基.
语种：
中文
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Degradation of anthocyanins and polymeric color formation during heat treatment of purple sweet potato extract at different pH
作者：
Jiang, Tian;Mao, Ying;Sui, Lushan;Yang, Ning;Li, Shuyi;...
期刊：
Food Chemistry ,2019年274:460-470 ISSN：0308-8146
通讯作者：
He, Jingren;He, Yi
作者机构：
[He, Yi; He, Jingren; Yin, Sheng; Li, Shuyi; Mao, Ying; Zhu, Zhenzhou; Sui, Lushan; Jiang, Tian; Yang, Ning] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Wang, Chengtao] BTBU, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.;[Yin, Sheng] Beijing Technol & Business Univ, Beijing Lab Food Qual & Safety, Beijing 100048, Peoples R China.
通讯机构：
[He, JR; He, Y] W;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Anthocyanins;Color changes;Degradation kinetics;Polymerization;Purple sweet potato extract
摘要：
Purple sweet potato anthocyanins are common natural pigments widely used in food industry, while they are often thermally processed in application. Degradation of anthocyanins, formation of polymers and color changes of purple sweet potato extract (PSPE) were investigated at 90 degrees C in the range of pH 3.0-pH 7.0. Data analysis indicated a first-order reaction for anthocyanins degradation in solutions with pH 3.0, 5.0 and 7.0 have half-lives of 10.27, 12.42 and 4.66h, respectively. The polymeric color formation followed zero-order kinetics, progressively increasing with pH values. The color of PSPE were changed with heating time and pH value through visual observation and colorimetric characterization. Analysis by UV-Vis spectrophotometry and HPLC indicated that anthocyanins in solution with pH 3.0 changed from monomeric anthocyanin into new polymers during heat treatment. Degradation of anthocyanins was accompanied by an increase in polymeric color index, due to the formation of melanoidin pigments and condensation reactions.
语种：
英文
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Facile Production of Purple Sweet Potato Porous Starch as Low Cost Food-based Adsorbent for Microencapsulation
作者：
Jiang, Fang-Cheng;Zhou, Rui;Cai, Jie* ;Dong, Qi;Ding, Wen-Ping;...
期刊：
BIORESOURCES ,2019年14(1):1252-1264 ISSN：1930-2126
通讯作者：
Cai, Jie;Liu, Gang;Wang, Guo-Zhen
作者机构：
[Liu, Gang; Cai, Jie; Liu, G; Wang, GZ; Zhou, Rui; Chen, Xuan; Dong, Qi; Jiang, Fang-Cheng; Wang, Guo-Zhen; He, Jing-Ren; Ding, Wen-Ping] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Liu, Gang; Cai, Jie; Liu, G; Wang, GZ; Wang, Guo-Zhen; He, Jing-Ren; Ding, Wen-Ping] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan, Hubei, Peoples R China.;[Liu, Gang; Cai, Jie; Liu, G; Wang, GZ; Wang, Guo-Zhen; He, Jing-Ren; Ding, Wen-Ping] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Wang, Hai-Bo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Cai, J; Liu, G; Wang, GZ] W;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan, Hubei, Peoples R China.;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.
关键词：
Adsorption;Complex enzymatic hydrolysis;Porous purple sweet potato starch
摘要：
This study aimed to develop a facile synthesis process of porous starch wall materials from purple sweet potato (PSP) for microencapsulation via enzymatic treatment. The optimum extraction conditions of purple sweet potato starch (PSP-S) were first attained by an orthogonal experiment. Response surface methodology was performed through the enzymatic hydrolysis of PSP-S using an α-amylase and glucoamylase complex to optimize the process parameters for the production of porous starch. Optimal reaction conditions were: the mass ratio (w/w) of glucoamylase to α-amylase of 6.39, amount of substrate of 19.5 g, and amount of enzymes of 0.53%. The surface morphology, microstructure, and thermal stability of the obtained samples were characterized with scanning electron microscopy, Fourier-transform infrared, and thermogravimetric analyses, respectively. The purple sweet potato porous starch (PSP-PS) had a stable oil adsorption capacity, and the molecular structure and thermal stability of porous starch were not substantially different from those of native starch. This study offers a simple yet efficient approach to produce fully biodegradable food-based porous materials for potential applications in oil microencapsulation. © 2019 North Carolina State University.
语种：
英文
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Recent advances in valorization of Chaenomeles fruit: A review of botanical profile, phytochemistry, advanced extraction technologies and bioactivities
作者：
Zhang, Rui;Li, Shuyi;Zhu, Zhenzhou* ;He, Jingren
期刊：
Trends in Food Science & Technology ,2019年91:467-482 ISSN：0924-2244
通讯作者：
Zhu, Zhenzhou
作者机构：
[Li, Shuyi; Zhu, Zhenzhou; Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.;[Li, Shuyi; Zhu, Zhenzhou; He, Jingren] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhu, Zhenzhou] W;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
Bioactivities;Chaenomeles;Extraction;Mugua;Phytochemistry
摘要：
Background: The genus Chaenomeles Lindley (Chaenomeles), known as Mugua (in Chinese), is a medicinal and edible plant that is considered a functional source for human health promoting. Numerous bio-, chemical- and physical technologies have been applied to explore the relationship between the phytochemistry of Chaenomeles and their bioactivities, opening the door to their potential application in the field of function food and traditional Chinese medicine. Scope and approach: This paper aimed to systematically summarize available information on Chaenomeles and provide reference for further investigations. The extracted and isolated chemical constituents (e.g., triterpenoids, sesquiterpenoids, flavonoids, organic acids, phenolic compounds, volatile oil and fatty acids) from five species of Chaenomeles were mainly summarized. This review highlights the theoretical aspects and recent developments of nonconventional extraction (microwave, ultrasound and enzyme-assisted extraction, super- and subcritical fluid extraction and solid phase extraction) technologies for bioactive compounds recovery from Chaenomeles. Additionally, scientific reports on crude extracts or pure compounds from this plant revealed broad bioactivities, including antioxidant, antimicrobial, antiinflammatory activities, as well as immunoregulatory and hepatoprotective effects. Key findings and conclusions: Approximately 150 chemicals have been characterized from different parts of Chaenomeles. Triterpenoids, flavonoids and phenolic compounds are the major bioactive ingredients of this plant. Additionally, the crude extracts and monomer compounds have been demonstrated with various in vitro and in vivo pharmacological functions. Nonconventional extraction technologies have been proposed to provide efficient, systematic, and practical protocols for the sustainable valorization of Chaenomeles. © 2019 Elsevier Ltd
语种：
英文
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气相色谱-质谱联用法测定植物油中脂肪酸组成
作者：
邓莉;何静仁;何毅;舒静
期刊：
中国调味品 ,2019年44(6):157-159,167 ISSN：1000-9973
作者机构：
武汉轻工大学食品科学与工程学院,湖北省农产品加工与转化重点实验室,武汉430023;[何毅; 何静仁; 邓莉; 舒静] 武汉轻工大学
关键词：
气相色谱-质谱联用;植物油;脂肪酸
摘要：
建立气相色谱-质谱联用法测定植物油中脂肪酸组成的方法。选用硼化氟-甲醇体系对样品进行甲酯化处理,再通过气相色谱-质谱技术对脂肪酸组成进行分析和鉴定。共分离出11种脂肪酸,结果表明:植物油中的脂肪酸以油酸、亚油酸、棕榈酸、硬脂酸为主,其中油酸和亚油酸含量均达70%以上,不饱和脂肪酸含量与饱和脂肪酸含量的比值:玉米油5.9%,菜籽油17.6%,葵花籽油11.7%,花生油3%,调和油5%。植物油中不饱和脂肪酸含量远远高于饱和脂肪酸含量。
语种：
中文
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芝麻油体的稳定性及油体膜蛋白结构分析
作者：
王智丰;雷帆;武艺;潘仪君;王国珍;...
期刊：
食品科技 ,2019年44(8):190-196 ISSN：1005-9989
作者机构：
武汉轻工大学食品科学与工程学院,武汉,430023;[王智丰; 金伟平; 武艺; 雷帆; 黄庆荣; 潘仪君; 何静仁; 王国珍] 武汉轻工大学
关键词：
油体;白芝麻;稳定性;凝结;油体蛋白;油体钙蛋白
摘要：
油体是天然存在于油料作物种子中的储油器官,了解其稳定性和表面膜蛋白结构对油体的应用至关重要。选取白芝麻油体为研究对象,考察不同加工与贮藏条件（如p H、离子强度、温度）对芝麻油体稳定性的影响,并对芝麻油体膜蛋白进行结构表征。结果表明:酸性环境（pH≤5.0）和高盐离子浓度（≥0.2 mol/L）会加速油体的聚集和失稳,而碱性条件、高热处理和低盐离子浓度则有助于维持其稳定性。通过聚丙烯酰胺凝胶电泳（SDS-PAGE）、红外光谱（FTIR）及差示扫描量热法（DSC）分析,发现白芝麻的油体膜蛋白主要由12～14 ku的油体蛋白（Oleosin）和27 ku的油体钙蛋白（Caleosin）组成,油体膜蛋白的热变性温度约为55℃,α-螺旋为其主要二级结构。这些参数为开发基于芝麻油体和油体膜蛋白的产品提供了理论参考。
语种：
中文
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Research advances of purple sweet potato anthocyanins: Extraction, identification, stability, bioactivity, application, and biotransformation
作者：
Li, Aoran;Xiao, Ruoshi;He, Sijia;An, Xiaoyu;He, Yi* ;...
期刊：
Molecules ,2019年24(21):3816 ISSN：1420-3049
通讯作者：
He, Yi;He, Jingren;Shi, Xuewei
作者机构：
[Xiao, Ruoshi; Li, Aoran; He, Yi; An, Xiaoyu; Yin, Sheng; He, Jingren; He, Sijia] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[He, Yi; Yin, Sheng; He, Jingren] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[He, Yi; Yin, Sheng; He, Jingren] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Wang, Chengtao; Yin, Sheng] BTBU, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.;[Shi, Xuewei; Wang, Bin] Shihezi Univ, Food Coll, Shihezi 832000, Peoples R China.
通讯机构：
[He, Y; He, JR] W;[He, Y; He, JR] M;[Shi, Xuewei] S;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.
关键词：
anthocyanin;antimutagenic agent;antineoplastic agent;antioxidant;animal;biotransformation;chemistry;human;isolation and purification;liver;metabolism;sweet potato;Animals;Anthocyanins;Antimutagenic Agents;Antineoplastic Agents, Phytogenic;Antioxidants;Biotransformation;Humans;Ipomoea batatas;Liver
摘要：
Purple sweet potato anthocyanins are kinds of natural anthocyanin red pigments extracted from the root or stem of purple sweet potato. They are stable and have the functions of anti-oxidation, anti-mutation, anti-tumor, liver protection, hypoglycemia, and anti-inflammation, which confer them a good application prospect. Nevertheless, there is not a comprehensive review of purple sweet potato anthocyanins so far. The extraction, structural characterization, stability, functional activity, application in the food, cosmetics, medicine, and other industries of anthocyanins from purple sweet potato, together with their biotransformation in vitro or by gut microorganism are reviewed in this paper, which provides a reference for further development and utilization of anthocyanins. © 2019 by the authors.
语种：
英文
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紫薯花色苷在贮藏过程中的降解特性
作者：
江甜;李佳;杨宁;何毅;祝振洲;...
期刊：
食品科学 ,2019年40(7):88-94 ISSN：1002-6630
作者机构：
武汉轻工大学食品科学与工程学院;[杨新笋] 湖北省农业科学院粮食作物研究所;[何毅; 祝振洲; 杨宁; 江甜; 何静仁; 李佳; 李书艺] 武汉轻工大学
关键词：
紫薯;花色苷;贮藏稳定性;降解特性;褐变指数;聚合物颜色指数
摘要：
本研究通过高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)法分析鉴定‘鄂薯12号'紫薯的花色苷成分,采用pH示差法和HPLC法研究该紫薯花色苷提取液在4、20、35 ℃贮藏98 d期间总花色苷和各单体花色苷的变化规律,并在此基础上研究了花色苷的降解动力学以及褐变指数和聚合物颜色指数。结果表明:从‘鄂薯12号'紫薯提取物中鉴定出13种花色苷,主要为矢车菊素-3-槐糖苷-5-葡糖苷和芍药素-3-槐糖苷-5-葡糖苷与对羟基苯甲酸、阿魏酸或咖啡酸形成的酰基化花色苷;贮藏期间总花色苷和各单体花色苷的含量呈下降趋势,花色苷的降解符合-级动力学模型;4、20 ℃和35 ℃贮藏条件下总花色苷半衰期分别为228.8、48.1 d和32.6 d;在相同糖苷配体情况下,矢车菊素类花色苷的半衰期要短于芍药素;在相同花色素配体情况下,酰基化花色苷的半衰期要长于未酰基化花色苷,且二酰化花色苷的半衰期长于单酰化花色苷;褐变指数和聚合物颜色指数随贮藏时间的延长和贮藏温度的升高而增大,并且聚合物颜色指数与花色苷含量之间呈指数关系。
语种：
中文
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Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.
作者：
He, Yi* ;Wang, Bin;Chen, Wanping;Cox, Russell J.;He, Jingren* ;...
期刊：
Biotechnology Advances ,2018年36(3):739-783 ISSN：0734-9750
通讯作者：
He, Yi;He, Jingren;Chen, Fusheng
作者机构：
[He, Yi; He, Y; He, Jingren] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[He, Yi; He, Jingren] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[He, Yi; He, Jingren] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Wang, Bin] Shihezi Univ, Food Coll, Xinjiang 832000, Uygur Autonomou, Peoples R China.;[Chen, Fusheng; Chen, Wanping] Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Wuhan 430070, Hubei, Peoples R China.
通讯机构：
[He, Y; He, JR] W;[Chen, Fusheng] H;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Wuhan 430070, Hubei, Peoples R China.
关键词：
*Aspergillus spp.;*Combinatorial biosynthesis;*DNA assembly;*Heterologous expression;*Microbial secondary metabolites;*Pathway engineering
摘要：
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
语种：
英文
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紫菜薹花色苷对内源性N-亚硝胺合成的抑制作用
作者：
邓莉;何静仁
期刊：
食品科技 ,2018年43(5):298-301 ISSN：1005-9989
作者机构：
武汉轻工大学食品科学与工程学院,湖北省农产品加工与转化重点实验室,武汉 430023;[何静仁; 邓莉] 武汉轻工大学
关键词：
紫菜薹;花色苷;N-亚硝胺;抑制作用
摘要：
在N-亚硝胺合成过程中,通过添加不同含量的紫菜薹花色苷,研究其对强致癌物N-亚硝胺合成的抑制作用。结果表明,当添加的紫菜薹花色苷含量小于0.8 mg/m L时,随着添加的紫菜薹花色苷含量增加,抑制效果越来越显著;当添加量等于0.8 mg/m L时,抑制率为62.184%,抑制效果最显著;当添加量大于0.8 mg/m L时,抑制效果基本维持不变。紫菜薹花色苷对N-亚硝胺合成有显著的抑制作用。
语种：
中文
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From ‘green’ technologies to ‘red’ antioxidant compounds extraction of purple corn: a combined ultrasound–ultrafiltration–purification approach
作者：
Jiang, Tian;Zhan, Shaoying;Li, Shuyi;Zhu, Zhenzhou;He, Jingren* ;...
期刊：
Journal of the Science of Food and Agriculture ,2018年98(13):4919-4927 ISSN：0022-5142
通讯作者：
He, Jingren;Lorenzo, Jose M.
作者机构：
[Zhan, Shaoying; Li, Shuyi; Zhu, Zhenzhou; Jiang, Tian; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Lorenzo, Jose M.] Ctr Tecnol Carne, Orense, Spain.;[Barba, Francisco J.] Univ Valencia, Fac Pharm, Food Sci Toxicol & Forens Med Dept, Nutr & Food Sci Area,Prevent Med & Publ Hlth, Avda Vicent Andres Estelles, Valencia, Spain.
通讯机构：
[He, Jingren] W;[Lorenzo, Jose M.] C;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.;Ctr Tecnol Carne, Orense, Spain.
关键词：
anthocyanin;antioxidant;plant extract;zein;chemistry;electrospray mass spectrometry;evaluation study;green chemistry;high performance liquid chromatography;isolation and purification;maize;procedures;ultrafiltration;Anthocyanins;Antioxidants;Chromatography, High Pressure Liquid;Green Chemistry Technology;Plant Extracts;Spectrometry, Mass, Electrospray Ionization;Ultrafiltration;Zea mays;Zein
摘要：
BACKGROUND: A pilot scale process consisting of ultrasound-assisted extraction, ammonium sulfate precipitation, cross-flow ultrafiltration and AB-8 macroporous resins purification aiming to recover anthocyanins and zein from purple corn (PC) was optimized and scaled-up. The effects of five independent variables (ethanol concentration, liquid to solid ratio, ultrasound temperature, time and power) were discussed and the most influential factors were optimized. RESULTS: The highest total anthocyanin (0.45 ± 0.01 g kg−1) and zein (17.14 ± 1.73 g kg−1) contents from purple corn were obtained using an ultrasound power of 105 W, an extraction time of 90 min, an ethanol concentration of 74% and a liquid to solid ratio of 26:1, at 70 °C, and this was consistent with the predicted values (0.46 and 17.36 g kg−1, for anthocyanin and zein, respectively). Subsequently, ammonium sulfate precipitation was used to isolate anthocyanins and zein. After cross-flow ultrafiltration, zein (6.30 g) was obtained with 80% purity. Anthocyanins were purified by AB-8 macroporous resins, resulting in 1.60 g of anthocyanins. High-performance liquid chromatography-electrospray ionization-mass spectrometry analysis revealed eight different anthocyanins in purple corn extracts. CONCLUSION: From the results obtained in the present study, it can be concluded that the proposed extraction–separation–filtration–purification method applied under the optimal conditions could be scaled-up to recover anthocyanins and zein simultaneously. Moreover, under the selected conditions, no significant degradation of anthocyanins was observed. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry
语种：
英文
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“食品分析”实验教学的探索与实践
作者：
邓莉;何静仁
期刊：
农产品加工 ,2018年(6):92-94+97 ISSN：1671-9646
作者机构：
武汉轻工大学食品科学与工程学院,湖北省农产品加工与转化重点实验室,湖北武汉430023;[何静仁; 邓莉] 武汉轻工大学
关键词：
食品分析;实验;改进措施;实践
摘要：
＂食品分析＂实验是食品科学与工程学科相关专业的核心必修课程之一,是＂食品分析＂课程实践和教学的重要组成部分。如何通过对实验教学探索,加强食品专业学生对理论知识的理解和掌握,培养其发现、分析和解决问题的能力是食品分析实验教学的目标。对＂食品分析＂实验教学中存在的一些显著问题,提出了一些建设性的措施,在满足教学任务的同时,对课程的教学模式进行了探索,并提出务实的改进措施。实践证明,这些措施在培养学生的实践和创新精神方面效果较为明显。
语种：
中文
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Bamboo cellulose-derived cellulose acetate for electrospun nanofibers: synthesis, characterization and kinetics
作者：
Cai, Jie* ;Zhou, Rui;Li, Tiantian;He, Jingren* ;Wang, Guozhen;...
期刊：
Cellulose ,2018年25(1):391-398 ISSN：0969-0239
通讯作者：
Cai, Jie;He, Jingren;Xiong, Hanguo
作者机构：
[Zhou, Rui; Cai, Jie; He, Jingren; Li, Tiantian; Wang, Guozhen] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Cai, Jie; Xiong, Hanguo] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Wang, Haibo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Cai, J; He, JR] W;[Xiong, Hanguo] H;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.
关键词：
Cellulose;Electrospinning;Nanofiber
摘要：
Catalysts play a key role in the production of cellulose acetate (CA). In this study, CA derived from bamboo cellulose (BC) was synthesized in a solution mixture of acetic acid and acetic anhydride that contains catalysts. The catalytic activities of three catalysts (i.e., sulfuric, methyl benzene sulfonic, and dodecyl benzene sulfonic acids) were investigated in terms of kinetics of acetylation, which was established according to the first-order reaction mechanism. Based on this, cellulose-based nanofibers were further prepared from the acetylated BC solution using an electrospinning technique. Results in this study may be useful for the preparation and application of nanomaterials derived from natural polymeric materials. © 2017, Springer Science+Business Media B.V., part of Springer Nature.
语种：
英文
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Enzyme-assisted extraction of polyphenol from edible lotus (Nelumbo nucifera) rhizome knot: Ultra-filtration performance and HPLC-MS2 profile
作者：
Zhu, Zhenzhou;Li, Shuyi* ;He, Jingren;Thirumdas, Rohit;Montesano, Domenico;...
期刊：
Food Research International ,2018年111:291-298 ISSN：0963-9969
通讯作者：
Li, Shuyi;Barba, Francisco J.
作者机构：
[Li, SY; Barba, FJ; Li, Shuyi; Zhu, Zhenzhou; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Thirumdas, Rohit] PJTSAU, Coll Food Sci & Technol, Dept Food Proc Technol, Hyderabad, Telangana, India.;[Montesano, Domenico] Univ Perugia, Dipartimento Sci Farmaceut, Sez Sci Alimenti & Nutr, Via S Costanzo 1, Perugia, Italy.;[Barba, Francisco J.] Univ Valencia, Fac Pharm, Prevent Med & Publ Hlth, Food Sci Toxicol & Forens Med Dept,Nutr & Food Sc, Ave Vicent Andres Estellas S-N, E-46100 Valencia, Spain.
通讯机构：
[Li, SY; Barba, FJ] W;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
*Enzyme;*Filtration;*HPLC-MS(2);*Lotus;*Polyphenol;*Rhizome knot
摘要：
Rhizome knot is always wasted as useless and inedible part of lotus root, despite its abundance of polyphenols. In this work, enzyme-assisted extraction followed by ultra-filtration was investigated to recover polyphenols from rhizome knot. Cellulase and pectinase treatment enhanced the polyphenols extraction. The 100 kDa membrane resulted in better filtration yield than 50 kDa membrane, 3.84% and 3.37%, respectively. With 100 kDa membrane, the highest filtration yield (4.08%) was achieved with a rotational speed of 600 rpm, TMP of 0.3 MPa and pH of 5. Satisfied permeate turbidity (<3 NTU) and polyphenol transmission (>90%) were obtained under these conditions. The main polyphenols identified in both rhizome knot extract and permeate were: chlorogenic acid, B-type procyanidin dimer·H<inf>2</inf>O, (+)-Catechin, B-type procyanidin dimer, (−)-Epicatechin, propyl gallate·H<inf>2</inf>O, caffeic acid, (−)-Epicatechin-3-gallate, and rutin. Membrane fouling led to the most important resistance (58% of total resistance). Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) evidenced that protein accumulation was the main fouling cause.<br/> ©2018
语种：
英文
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