天冬酰胺通过TLR4和NOD信号通路对LPS诱导的仔猪肝脏损伤的调控作用
作者:
吴欢听;刘玉兰;石海峰;李爽;皮定安;...
作者机构:
[吴欢听; 刘玉兰; 石海峰; 李爽; 皮定安; 冷炜博; 朱慧玲; 侯永清] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
天冬酰胺;仔猪;肝脏损伤;炎性介质
摘要:
本试验研究了天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肝脏损伤的保护作用,并从toll样受体4(TLR4)和核苷酸结合寡聚域蛋白(NOD)信号通路的角度探讨其机理。选取48头断奶仔猪,分为4个处理:(1)对照组(基础日粮);(2)LPS组(基础日粮+LPS);(3)0.5%Asn组(基础日粮+0.5%Asn+LPS);(4)1.0%Asn组(基础日粮+1.0%Asn+LPS)。实验第20天,2、
语种:
中文
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益生菌对脂多糖刺激仔猪组织中有关基mRNA表达量的影响
作者:
赵迪;吴涛;侯永清;王蕾;曾娟娟;...
作者机构:
[赵迪; 吴涛; 侯永清; 王蕾; 曾娟娟; 窦茂鑫] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
芽孢杆菌;干酪乳杆菌;脂多糖;仔猪;mRNA表达
摘要:
益生菌可抑制机体中有害菌生长,促进优势菌群增殖,增强非特异性免疫功能,从而促进动物生长、增强机体免疫力、提高饲料转化率,产生与抗生素相似的作用效果。本试验旨在研究复合芽孢杆菌和干酪乳杆菌对脂多糖(LPS)刺激仔猪肠道、肝脏等组织免疫应激的缓解作用,探讨其在mRNA水平的调控机制。试验选取28日龄(杜×长×大)健康仔猪24头,随机分为四个处理组(空白对照组、免疫应激组、复合芽孢组、干酪乳杆菌组),每
语种:
中文
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3种饲用植物精油及其主要成分对猪源致病菌的抑菌作用研究
作者:
汪中兴;侯永清;窦茂鑫;王蕾;易丹;...
期刊:
饲料研究 ,2014年(13):42-45 ISSN:1002-2813
作者机构:
武汉轻工大学动物科学与营养工程学院;动物营养与饲料科学湖北省重点实验室;[窦茂鑫] 深圳市金新农饲料股份有限公司;[肖航; 侯永清; 王蕾; 方秋红; 汪中兴; 易丹; 赵迪] 武汉轻工大学
关键词:
肉桂油;牛至油;百里香油;大肠杆菌K88;霍乱沙门菌A72;最小抑菌质量浓度
摘要:
采用高效液相色谱法,检测3种可饲精油(肉桂油、牛至油和百里香油)中主要成分的含量,用微板指示剂法测定3种精油及其主要成分对猪源产肠毒素大肠杆菌K88和霍乱沙门菌A72的体外抑菌活力.结果表明:肉桂油及其主要成分的抑菌活力最强,最小抑菌质量浓度(MIC)为400 ~800 μL/L;百里香油及其主要成分百里香酚的作用次之,MIC为1 600 μL/L;牛至油及其主要成分香芹酚的作用最差,MIC为1 600 ~3 200μL/L.
语种:
中文
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几种氨基酸及其衍生物对猪肠上皮细胞IPEC-1细胞活力与生长的影响
作者:
欧阳万金;龙民慧;侯永清;肖航;丁斌鹰;...
期刊:
饲料工业 ,2014年35(3):42-46 ISSN:1001-991X
作者机构:
动物营养与饲料科学湖北省重点实验室武汉轻工大学,湖北武汉,430023
关键词:
氨基酸及其衍生物;细胞活力
摘要:
研究L-谷氨酰胺(L-glutamine,GLN),Nα-乙酰-L-谷氨酰胺(Nα-acetyl-L-glutamine,NAG),L-精氨酸(L-arginine,ARG),N-α-乙酰-L-精氨酸(N-alpha-acetyl-L-arginine,NAA)、瓜氨酸(L-citrulline,CIT),α-酮戊二酸(Alpha-ketoglutaric acid,AKG)和L-脯氨酸(L-Proline,PRO)对猪小肠上皮细胞(Intestinal porcine epithelial cells,IPEC-1)细胞活力与生长的影响,比较这几种氨基酸及其衍生物对IPEC-1细胞生长的作用效果。在IPEC-1定制培养液中分别添加不同浓度的氨基酸或其衍生物培养4 d,用CCK8(cell counting kit 8)法检测细胞的活力,并用细胞计数法检测这些氨基酸或其衍生物对细胞生长的影响。试验的整个4 d,GLN组IPEC-1细胞的数量(P<0.05)和OD值(P<0.05)均显著高于其他组。在试验的第4 d,NAG、AKG、CIT、NAA和ARG组细胞数量均高于对照组(P<0.05或0.01)。培养液中添加PRO对IPEC-1细胞的生长无明显影响。①在这几种氨基酸及其衍生物中,GLN促进IPEC-1细胞生长的作用效果最好。②NAG、AKG、CIT、NAA或ARG均能提高第4 d IPEC-1细胞的数量。
语种:
中文
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Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid
作者:
Hou, Yongqing* ;Wang, Lei;Yi, Dan;Ding, Binying;Chen, Xing;...
期刊:
British Journal of Nutrition ,2014年111(10):1748-1758 ISSN:0007-1145
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Hou, Yongqing; Chen, Xing; Wang, Lei; Wang, Qingjing; Ding, Binying; Yin, Yulong; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Acetic acid;Colon;Intestinal injury;Piglets;Tributyrin
摘要:
Tributyrin (TBU) is a good dietary source of butyrate and has beneficial effects on the maintenance of normal intestinal morphology. The present study tested the hypothesis that dietary TBU supplementation could alleviate intestinal injury in the acetic acid (ACA)-induced porcine model of colitis. A total of eighteen piglets (25Â d old) were randomly allocated to one of three treatment groups (control, ACA and TBU). The control and ACA groups were fed a basal diet and the TBU group was fed the basal diet supplemented with 0·1Â % TBU. On day 15 of the trial, under anaesthesia, a soft catheter was inserted into the rectum of piglets (20-25Â cm from the anus), followed by administration of either saline (control group) or ACA (10Â ml of 10Â % ACA solution for ACA and TBU groups). On day 22 of the trial, after venous blood samples were collected, piglets were killed to obtain mid-ileum and mid-colon mucosae. Compared with the control group, the ACA group exhibited an increase (P<Â 0·05) in lymphocyte counts, creatinine, PGE2, and malondialdehyde concentrations and diamine oxidase and inducible NO synthase activities in the plasma and lymphocyte density in the colon and a decrease in insulin concentrations and glutathione peroxidase activity, ileal villus height:crypt depth ratios and goblet cell numbers in the colon. These adverse effects of ACA were attenuated by TBU supplementation. Moreover, TBU prevented the ACA-induced increase in caspase-3 levels while enhancing claudin-1 protein and epidermal growth factor receptor (EGFR) mRNA expression in the colonic mucosa. Collectively, these results indicate that dietary supplementation with 0·1Â % TBU alleviates ACA-induced intestinal injury possibly by inhibiting apoptosis, promoting tight-junction formation and activating EGFR signalling. © 2014 The Authors.
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英文
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Chlorogenic Acid Decreases Intestinal Permeability and Increases Expression of Intestinal Tight Junction Proteins in Weaned Rats Challenged with LPS
作者:
Ruan, Zheng;Liu, Shiqiang;Zhou, Yan;Mi, Shumei;Liu, Gang;...
期刊:
PLOS ONE ,2014年9(6):e97815 ISSN:1932-6203
通讯作者:
Wu, Guoyao
作者机构:
[Zhou, Yan; Mi, Shumei; Deng, Zeyuan; Yin, Yulong; Ruan, Zheng; Liu, Shiqiang] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China.;[Liu, Gang; Yao, Kang; Wu, Xin; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.;[Assaad, Houssein] Texas A&M Univ, Dept Stat, College Stn, TX 77843 USA.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.;[Hou, Yongqing; Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Wu, Guoyao] T;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
关键词:
Gastrointestinal tract;Jejunum;Colon;Permeability;Diet;Tight junctions;Ileum;Urine
摘要:
Chlorogenic acid, a natural phenolic acid present in fruits and plants, provides beneficial effects for human health. The objectives of this study were to investigate whether chlorogenic acid (CHA) could improve the intestinal barrier integrity for weaned rats with lipopolysaccharide (LPS) challenge. Thirty-two weaned male Sprague Dawley rats (21 +/- 1 d of age; 62.26 +/- 2.73 g) were selected and randomly allotted to four treatments, including weaned rat control, LPS-challenged and chlorogenic acid (CHA) supplemented group (orally 20 mg/kg and 50 mg/kg body). Dietary supplementation with CHA decreased (P < 0.05) the concentrations of urea and albumin in the serum, compared to the LPS-challenged group. The levels of IFN-gamma and TNF-alpha were lower (P < 0.05) in the jejunal and colon of weaned rats receiving CHA supplementation, in comparison with the control group. CHA supplementation increased (P < 0.05) villus height and the ratio of villus height to crypt depth in the jejunal and ileal mucosae under condictions of LPS challenge. CHA supplementation decreased (P < 0.05) intestinal permeability, which was indicated by the ratio of lactulose to mannitol and serum DAO activity, when compared to weaned rats with LPS challenge. Immunohistochemical analysis of tight junction proteins revealed that ZO-1 and occludin protein abundances in the jejunum and colon were increased (P < 0.05) by CHA supplementation. Additionally, results of immunoblot analysis revealed that the amount of occludin in the colon was also increased (P < 0.05) in CHA-supplemented rats. In conclusion, CHA decreases intestinal permeability and increases intestinal expression of tight junction proteins in weaned rats challenged with LPS.
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英文
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mTOR信号通路在谷氨酰胺调控猪肠上皮细胞生长中的作用
作者:
欧阳万金;肖航;侯永清;龙民慧;丁斌鹰;...
作者机构:
[欧阳万金; 肖航; 侯永清; 龙民慧; 丁斌鹰; 易丹; 王蕾] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
谷氨酰胺;猪小肠上皮细胞;mTOR信号通路
摘要:
谷氨酰胺(L-glutamine,Gln)是肠细胞和免疫细胞等细胞快速分化的优先底物,可刺激细胞增殖,能调控基因表达和细胞信号通路。Gln不仅作为肠上皮细胞的主要能量物质,为小肠上皮细胞的分化和增殖提供能量,而且还可通过调节谷氨酰胺酶活性、相关细胞信号通路和蛋白合成等促进肠上皮细胞的增殖,但其作用机理还不清楚。mTOR(哺乳动物雷帕霉素靶蛋白,mammalian target of rapamyc
语种:
中文
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复合芽孢杆菌对脂多糖刺激仔猪抗氧化能力的影响
作者:
曾娟娟;赵迪;王磊;王蕾;侯永清;...
作者机构:
[曾娟娟; 赵迪; 王磊; 王蕾; 侯永清; 吴涛] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
复合芽孢杆菌;仔猪;抗氧化能力;脂多糖
摘要:
芽孢杆菌是一类好氧菌,能在不利生长的条件下产生芽孢,不仅具有耐高温,耐高压,耐酸碱和耐胆盐等生物学特性,而且能够适应颗粒料的生产加工工艺,不变质,储存时间长。本试验选用的复合芽孢杆菌是通过生物学特性研究筛选出来的地衣芽孢杆菌和枯草芽孢杆菌混合物。试验研究了复合芽孢杆菌对脂多糖刺激仔猪抗氧化能力的影响。选用18头体重相近、健康的28日龄仔猪(杜洛克×长白×大白,平均体重6.32±1.14 kg),随
语种:
中文
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Aspartate alleviates liver injury and regulates mRNA expressions of TLR4 and NOD signaling-related genes in weaned pigs after lipopolysaccharide challenge
作者:
Leng, Weibo;Liu, Yulan* ;Shi, Haifeng;Li, Shuang;Zhu, Huiling;...
期刊:
JOURNAL OF NUTRITIONAL BIOCHEMISTRY ,2014年25(6):592-599 ISSN:0955-2863
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Pi, Dingan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Aspartate;Lipopolysaccharide;Liver injury;Nucleotide-binding oligomerization domain protein;Pro-inflammatory cytokines;Toll-like receptor 4;Weaned pigs
摘要:
Pro-inflammatory cytokines play a critical role in many models of liver injury. In addition, aspartate (Asp) plays an important role in many biological and physiological processes including liver physiology. We hypothesized that Asp could alleviate lipopolysaccharide (LPS)-induced liver injury. Forty-eight weanling pigs were assigned to four treatments including: (1) non-challenged control; (2) LPS challenged control; (3) LPS+0.5% Asp; (4) LPS+1.0% Asp. After 20-d feeding with control (0% Asp), 0.5% or 1.0% Asp supplemented diets, pigs were injected with saline or LPS. At 4 (early phase) and 24 h (late phase) post-injection, blood and liver samples were obtained. Asp attenuated liver injury indicated by reduced serum aspartate aminotransferase activity and increased ratio of serum alanine aminotransferase and aspartate aminotransferase at 24 h, and less severe histological liver damage induced by LPS challenge at 4 or 24 h. In addition, Asp supplementation to LPS challenged pigs decreased mRNA expressions of tumor necrosis factor (TNF)-alpha and cyclooxygenase-2 linearly and quadratically at 4 h, and increased mRNA expressions of these pro-inflammatory mediators linearly and quadratically at 24 h. Finally, Asp decreased mRNA expression of toll-like receptor 4 (TLR4) signaling related genes (TLR4, myeloid differentiation factor 88, IL-1 receptor-associated kinase 1, TNF-alpha receptor-associated factor (6), nucleotide-binding oligomerization domain protein (NOD) signaling related genes (NOD1, NOD2 and receptor-interacting serine/threonine-protein kinase 2) and nuclear factor-kappa B p65 linearly or quadratically at 4 h. However, Asp increased mRNA expressions of these signaling molecules linearly or quadratically at 24 h. These results indicate that, at early and late phases of LPS challenge, Asp exerts opposite regulatory effects on mRNA expression of hepatic pro-inflammatory cytokines and TLR4 and NOD signalling related genes, and improves liver integrity. (C) 2014 Elsevier Inc. All rights reserved.
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英文
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Reduction of salmonella enterica Serovar typhimurium DT104 infection in experimentally challenged weaned pigs fed a lactobacillus-fermented feed
作者:
Yin, Fugui;Farzan, Abdolvahab;Wang, Qi (Chuck);Yu, Hai;Yin, Yulong;...
期刊:
FOODBORNE PATHOGENS AND DISEASE ,2014年11(8):628-634 ISSN:1535-3141
通讯作者:
Gong, Joshua
作者机构:
[Yin, Fugui; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agri Ecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.;[Wang, Qi (Chuck); Gong, Joshua; Yin, Fugui; Yu, Hai] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Friendship, Robert; Farzan, Abdolvahab] Univ Guelph, Dept Populat Med, Guelph, ON N1G 2W1, Canada.;[Hou, Yongqing; Yin, Yulong] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构:
[Gong, Joshua] A;Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
关键词:
Food products;Salmonella
摘要:
Salmonella Typhimurium is a foodborne pathogen and commonly present on pig farms. Probiotics have shown potential as a means of reducing Salmonella shedding in pigs. Three experimental challenge trials were conducted to investigate the potential application of newly isolated Lactobacillus isolates for controlling Salmonella infection in pigs. In each trial, 16 Yorkshire piglets (28-d old) were randomly allocated to 1 of 4 dietary treatments: (1) basal diet (BD), (2) naturally fermented (NF) feed, (3) Lactobacillus zeae-fermented (LZ-F) feed, and 4) Lactobacillus casei-fermented (LC-F) feed. All pigs consumed their assigned diets for 3 d prior to the challenge of Salmonella Typhimurium DT104 (approximately 6 log colony-forming units/pig) through gavage. Pediococcus pentosaceus, L. zeae, and L. casei were most abundant in NF, LZ-F, and LC-F feed, respectively. After the challenge, pigs on fermented feed had lower rectal temperature, diarrhea scores, serum haptoglobin concentrations, and intestinal Salmonella counts than the control group (BD) (p≤0.01). Salmonella spp. were detected in both ileocecal lymph nodes (ICLN) and spleens from all pigs on BD, NF, and LC-F, but only 50% of spleens from pigs on LZ-F. Pigs had a dynamic spatial and temporal immune response to Salmonella infection and dietary treatments, as indicated by up- and downregulation in gene expression of inflammatory cytokines (interleukin (IL)-1β, IL-6, IL-10, interferon-γ, and tumor necrosis factor) in the ileum, ICLN, and spleen. The alternation in cytokine expression by fermented feed, particularly LZ-F, appeared to benefit pigs in combating Salmonella infection. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
语种:
英文
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天冬氨酸对脂多糖刺激断奶仔猪肠道形态结构和黏膜抗氧化能力的影响
作者:
冷炜博;刘玉兰;李爽;石海峰;朱惠玲;...
期刊:
中国畜牧杂志 ,2014年50(11):32-36 ISSN:0258-7033
作者机构:
武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;[侯永清; 李爽; 冷炜博; 皮定安; 朱惠玲; 刘玉兰; 石海峰] 武汉轻工大学
关键词:
天冬氨酸;脂多糖;肠黏膜;抗氧化;断奶仔猪
摘要:
本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激断奶仔猪肠道结构和黏膜抗氧化能力的影响.选取(21±1)d日龄断奶、平均体重(7.37±0.04)kg的杜×长×大三元杂交仔猪24头,随机分为对照组(基础日粮)、LPS组(基础日粮+LPS)、0.5% ASP组(基础日粮+0.5%ASP+LPS)、1.0% ASP组(基础日粮+1.0%ASP+LPS)4组,试验期24 d.试验第24天,分别给3个试验组的猪注射100 μg/kg体重的LPS,对照组注射等量的生理盐水.注射后24 h屠宰,取小肠样品待测.结果表明:0.5%或1.0% ASP可显著缓解LPS刺激导致的空肠绒毛高度降低、隐窝深度升高和绒毛高度/隐窝深度的降低,增加回肠的绒毛高度、绒毛高度/隐窝深度(P<0.05);0.5%ASP对空肠超氧化物歧化酶、回肠谷胱甘肽过氧化物酶活性有提高的趋势(P<0.10);0.5%或1.0% ASP显著降低了空肠和回肠丙二醛含量(P<0.05).以上研究结果显示,ASP有提高仔猪肠黏膜抗氧化相关酶的酶活趋势,降低过氧化物的产生,从而改善肠道形态结构.
语种:
中文
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N-乙酰半胱氨酸对猪肠道功能的保护作用
作者:
侯永清;王蕾;易丹
期刊:
动物营养学报 ,2014年26(10):3064-3070 ISSN:1006-267X
作者机构:
[侯永清; 王蕾; 易丹] 武汉轻工大学;武汉轻工大学动物营养与饲料安全湖北省协同创新中心
关键词:
N-乙酰半胱氨酸;猪;肠道功能
摘要:
动物的肠道完整性在营养、代谢和机体内环境稳定中起着非常重要的作用,是机体抵御内源性和外源性有害物质的第一道屏障。肠上皮细胞的完整性是保障肠道功能的生理基础,肠上皮受损会影响营养物质的吸收,从而降低生产性能和影响动物的健康。N-乙酰半胱氨酸(NAC)是L-半胱氨酸与还原型谷胱甘肽(GSH)的前体物质,NAC的生理功能和应用效果在很大程度上与维持细胞内的GSH水平有关。饲粮中添加NAC可以缓解脂多糖应激引起的肠黏膜损伤、乙酸刺激引起的结肠炎及改善小肠吸收功能,这些与NAC促进表皮生长因子的生成、抗黏膜细胞凋亡和促进紧密连接蛋白表达有关。本文就NAC对猪肠道功能的保护作用相关研究作一综述。
语种:
中文
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Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge
作者:
Pi, Dingan;Liu, Yulan* ;Shi, Haifeng;Li, Shuang;Odle, Jack;...
期刊:
JOURNAL OF NUTRITIONAL BIOCHEMISTRY ,2014年25(4):456-462 ISSN:0955-2863
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Chen, Feng; Pi, Dingan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Aspartate;Energy status;Intestine;Lipopolysaccharide;Weanling piglets
摘要:
The intestine has a high requirement for ATP to support its integrity, function and health, and thus, energy deficits in the intestinal mucosa may play a critical role in intestinal injury. Aspartate (Asp) is one of the major sources of ATP in mammalian enterocytes via mitochondrial oxidation. We hypothesized that dietary supplementation of Asp could attenuate lipopolysaccharide (LPS)-induced intestinal damage via modulation of intestinal energy status. Twenty-four weanling piglets were allotted to one of four treatments: (1) nonchallenged control, (2) LPS-challenged control, (3) LPS+0.5% Asp treatment, and (4) LPS+1.0% Asp treatment. On day 19, pigs were injected with saline or LPS. At 24 h postinjection, pigs were killed and intestinal samples were obtained. Asp attenuated LPS-induced intestinal damage indicated by greater villus height and villus height/crypt depth ratio as well as higher RNA/DNA and protein/DNA ratios. Asp improved intestinal function indicated by increased intestinal mucosal disaccharidase activities. Asp also improved intestinal energy status indicated by increased ATP, ADP and total adenine nucleotide contents, adenylate energy charge and decreased AMP/ATP ratio. In addition, Asp increased the activities of tricarboxylic acid cycle key enzymes including citrate synthase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase complex. Moreover, Asp down-regulated mRNA expression of intestinal AMP-activated protein kinase alpha 1 (AMPK alpha 1), AMPK alpha 2, silent information regulator 1 (SIRT1) and peroxisome proliferator activated receptor gamma coactivator-1 alpha (PGC1 alpha) and decreased intestinal AMPK alpha phosphorylation. These results indicate that Asp may alleviate LPS-induced intestinal damage and improve intestinal energy status. (C) 2014 Elsevier Inc. All rights reserved.
语种:
英文
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干酪乳杆菌对脂多糖刺激仔猪抗氧化能力的影响
作者:
曾娟娟;赵迪;王磊;王蕾;侯永清;...
作者机构:
[曾娟娟; 赵迪; 王磊; 王蕾; 侯永清; 吴涛] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
干酪乳杆菌;仔猪;抗氧化能力;脂多糖
摘要:
益生菌是一类有利于人体健康的活性微生物食品原料,干酪乳杆菌和嗜酸乳杆菌和双歧杆菌一起被称为健康三益菌。干酪乳杆菌具有降低血清胆固醇、抗肿瘤、防治腹泻和提高机体免疫力、降血压等益生功能,但国内对干酪乳杆菌的研究并不多。本试验旨在研究干酪乳杆菌对脂多糖(LPS)刺激仔猪肠道、肝脏等组织抗氧化能力的影响。选用18头体重相近、健康的28日龄仔猪(杜洛克×长白×大白,平均体重6.38±1.13 kg),随机
语种:
中文
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脂多糖刺激对仔猪下丘脑-垂体-肾上腺轴Toll样受体4信号通路关键基因表达的影响
作者:
陈少魁;刘玉兰;李权;朱惠玲;侯永清;...
期刊:
动物营养学报 ,2014年26(11):3356-3361 ISSN:1006-267X
作者机构:
[侯永清; 吴欢听; 李权; 王秀英; 陈少魁; 刘称; 朱惠玲; 刘玉兰] 武汉轻工大学
关键词:
仔猪;脂多糖;HPA轴;TLR4信号通路
摘要:
本试验旨在研究脂多糖(LPS)刺激对仔猪下丘脑-垂体-肾上腺(HPA)轴Toll样受体4(TLR4)信号通路关键基因表达的影响。选取12头断奶仔猪,分成2个组,每个组6个重复。试验组注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后采血,测定血浆中与应激相关激素的含量;采血后屠宰仔猪,取下丘脑、垂体、肾上腺,测定TLR4信号通路关键基因的mRNA表达水平,包括TLR4、髓样分化因子88(My D88)、白细胞介素-1受体相关激酶1(IRAK1)、肿瘤坏死因子受体相关因子6(TRAF6)和核转录因子-κB(NF-κB)mRNA表达水平。结果表明:与对照组相比,1)LPS刺激导致血浆肿瘤坏死因子-α、皮质醇和促肾上腺皮质激素含量显著上升(P<0.05)。2)LPS刺激导致TLR4信号通路关键基因TLR4在下丘脑、垂体、肾上腺中mRNA表达水平显著升高(P<0.05);My D88在垂体、肾上腺中mRNA表达水平显著升高(P<0.05),在下丘脑中mRNA表达水平有升高的趋势(P<0.10);IRAK1在垂体、肾上腺中mRNA表达水平有升高的趋势(P<0.10);TRAF6在下丘脑、垂体、肾上腺中mRNA表达水平显著升高(P<0.05);NF-κB在垂体中mRNA表达水平显著升高(P<0.05)。试验结果表明LPS刺激激活了HPA轴,诱导了HPA轴的TLR4信号通路关键基因的表达。
语种:
中文
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N-乙酰半胱氨酸对脂多糖刺激仔猪血浆中激素水平的影响
作者:
肖航;方秋红;侯永清;丁斌鹰;王蕾;...
作者机构:
[肖航; 方秋红; 侯永清; 丁斌鹰; 王蕾; 陈振强] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
N-乙酰半胱氨酸;脂多糖;仔猪;血浆;激素水平
摘要:
动物的生长是由促生长激素轴来调控的,促生长激素轴由生长激素释放因子(GRF)、生长激素(GH)和胰岛素样生长因子(IGF)构成,与动物的生长相关。IGF-1能够直接作用于动物体内的多种组织,促进蛋白质合成,促进细胞增殖,达到促进生长的作用。INS(胰岛素)是促进合成代谢、调节血糖稳定的主要激素,有促进动物体内蛋白质合成的作用。INS和IGF-1的水平提高意味着动物体合成代谢增强。胰高血糖素是促进分
语种:
中文
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Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets
作者:
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Ding, Binying;Yang, Zhengguo;...
期刊:
British Journal of Nutrition ,2014年111(1):46-54 ISSN:0007-1145
通讯作者:
Hou, Yongqing
作者机构:
[Long, Minhui; Liu, Yulan; Yang, Zhengguo; Hou, Yongqing; Wang, Lei; Ding, Binying; Li, Jiao; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
N-Acetylcysteine;Piglets;Lipopolysaccharide;Liver injury
摘要:
The present study was carried out to determine whether N-acetylcysteine (NAC) could modulate liver injury in a lipopolysaccharide (LPS)-challenged piglet model. For this purpose, eighteen piglets were randomly assigned to the control, LPS or NAC group. Piglets in the control and LPS groups were fed a basal diet, whereas those in the NAC group were fed the basal diet supplemented with 500mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS-and NAC-treated piglets were intraperitoneally administered LPS (100μg/kg body weight), while the control group was administered the same volume of saline. On day 20 of the trial, blood samples were obtained 3h after LPS or saline injection. On day 21, the piglets were killed to collect liver samples. Dietary NAC supplementation attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, in the LPS-challenged piglets, the activities of alanine aminotransferase and aspartate aminotransferase and the concentrations of H2O2, TNF-α, IL-6 and PGE2 were dramatically increased in the plasma and the activity of superoxide dismutase in the plasma and that of glutathione peroxidase in the liver were significantly decreased. The LPS challenge also increased the concentration of AMP and the ratio of AMP:ATP, but decreased adenylate energy charges and the levels of ATP and ADP. These adverse effects of the LPS challenge were ameliorated by NAC supplementation. Moreover, NAC inhibited the LPS-induced increases in the abundance of liver heat shock protein 70 and NF-κB proteins. In conclusion, these results suggest that dietary NAC supplementation alleviates LPS-induced liver injury by reducing the secretion of pro-inflammatory cytokines, increasing the antioxidative capacity and improving energy metabolism. © 2013 The Authors.
语种:
英文
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天冬酰胺对脂多糖刺激仔猪肠黏膜结构及抗氧化能力的影响
作者:
皮定安;刘玉兰;李爽;石海峰;朱惠玲;...
期刊:
中国畜牧杂志 ,2014年50(05):60-64 ISSN:0258-7033
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[丁斌鹰; 侯永清; 李爽; 冷炜博; 陈逢; 朱惠玲; 刘玉兰; 皮定安; 石海峰] 武汉轻工大学
关键词:
天冬酰胺;脂多糖;肠黏膜结构;抗氧化;仔猪
摘要:
试验旨在研究天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肠道结构及抗氧化能力的影响。选用24头断奶仔猪,分为对照组、LPS组、0.5%Asn组(0.5%Asn+LPS)和1.0%Asn组(1.0%Asn+LPS)。第24天,各组分别注射LPS或等量生理盐水24 h后屠宰。结果表明:LPS刺激导致空肠绒毛高度显著降低,日粮中添加0.5%或1.0%Asn显著降低了空肠、回肠的隐窝深度,提高了空肠、回肠的绒毛高度和绒毛高度/隐窝深度的比值(P<0.05);LPS刺激也导致空肠超氧化物歧化酶(SOD)活性和氧化型谷胱甘肽(GSSG)/还原性谷胱甘肽(GSH)比值显著升高(P<0.05);日粮中添加0.5%或1.0%Asn显著提高了空肠、回肠GSH的含量(P<0.05),降低了空肠、回肠GSSG/GSH的比值(P<0.01)和丙二醛(MDA)的含量(P<0.05)。结果显示,Asn缓解了LPS刺激引起的肠道结构损伤和抗氧化能力下降。
语种:
中文
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Effects of l-proline on the growth performance, and blood parameters in weaned lipopolysaccharide (LPS)-challenged pigs
作者:
Kang, Ping;Zhang, Lili;Hou, Yongqing* ;Ding, Binying;Yi, Dan;...
期刊:
ANIMAL BIOSCIENCE ,2014年27(8):1150-1156 ISSN:2765-0189
通讯作者:
Hou, Yongqing
作者机构:
[Zhang, Lili; Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Ding, Binying; Yin, Yulong; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
L-proline;Growth Performance;Blood Parameters;Gastrointestinal Tract Digestibility;Early Weaned Pigs;Lipopolysaccharide
摘要:
This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly to dietary treatments in a 2×3 factorial arrangement in a 20-d growth assay. Factors were intraperitoneal injection with saline or LPS, and three dietary L-proline supplement levels (0%, 0.5%, or 1.0%). On d 10, blood samples were collected at 3 h after LPS (100 μg LPS/kg body weight [BW]) or saline injection. On d 20 of the trial, all pigs were orally administrated D-xylose (0.1 g/kg BW) at 2 h, and blood samples were collected at 3 h after LPS or saline injection. As a result, dietary supplementation with 0.5% proline had a tendency to increase average daily gain (ADG) in piglets during d 10 to 20 (p = 0.088). Without LPS challenge, dietary supplementation with 1.0% proline had no effect on growth hormone (GH) concentrations on d 10 (p>0.05), but decreased it after LPS challenge (p<0.05). There was LPS challenge× proline interaction for GH concentrations on d 10 (p<0.05). Dietary supplementation with 1.0% proline decreased glucagon concentration on d 10 after LPS challenge (p<0.05). In addition, dietary supplementation with proline increased superoxide dismutase (SOD) activity significantly on d 10 and 20 (p<0.05), and 1.0% proline increased heat shock proteins-70 concentration on d 10 (p<0.05). Moreover, proline supplementation increased diamine oxidase (DAO) concentrations after LPS challenge (p<0.05). There was LPS challenge×proline interaction for DAO (p<0.05). Furthermore, dietary supplementation with 1.0% proline increased the D-xylose level when no LPS challenge (p<0.05). These results indicate that proline supplementation could improve growth performance, increase SOD activities, and has a positive effect on the gastrointestinal tract digestibility in early weaned pigs. © 2014 by Asian-Australasian Journal of Animal Sciences.
语种:
英文
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Dietary l-glutamine supplementation modulates microbial community and activates innate immunity in the mouse intestine
作者:
Ren, Wenkai;Duan, Jielin;Yin, Jie;Liu, Gang;Cao, Zhong;...
期刊:
Amino Acids ,2014年46(10):2403-2413 ISSN:0939-4451
通讯作者:
Xiong, Xia
作者机构:
[Liu, Gang; Yin, Jie; Ren, Wenkai; Duan, Jielin; Chen, Shuai; Xiong, Xia; Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.;[Liu, Gang; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410004, Hunan, Peoples R China.;[Hou, Yongqing; Wu, Guoyao; Yin, Yulong] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Xiong, Xia] C;Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.
关键词:
Glutamine;Paneth cell;Firmicutes;NF-kappa B;Innate immunity
摘要:
This study was conducted to determine effects of dietary supplementation with 1 % l-glutamine for 14 days on the abundance of intestinal bacteria and the activation of intestinal innate immunity in mice. The measured variables included (1) the abundance of Bacteroidetes, Firmicutes, Lactobacillus, Streptococcus and Bifidobacterium in the lumen of the small intestine; (2) the expression of toll-like receptors (TLRs), pro-inflammatory cytokines, and antibacterial substances secreted by Paneth cells and goblet cells in the jejunum, ileum and colon; and (3) the activation of TLR4-nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), and phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt) signaling pathways in the jejunum and ileum. In the jejunum, glutamine supplementation decreased the abundance of Firmicutes, while increased mRNA levels for antibacterial substances in association with the activation of NF-κB and PI3K-Akt pathways. In the ileum, glutamine supplementation induced a shift in the Firmicutes:Bacteroidetes ratio in favor of Bacteroidetes, and enhanced mRNA levels for Tlr4, pro-inflammatory cytokines, and antibacterial substances participating in NF-κB and JNK signaling pathways. These results indicate that the effects of glutamine on the intestine vary with its segments and compartments. Collectively, dietary glutamine supplementation of mice beneficially alters intestinal bacterial community and activates the innate immunity in the small intestine through NF-κB, MAPK and PI3K-Akt signaling pathways. © 2014 Springer-Verlag Wien.
语种:
英文
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