作者： Ren, Wenkai;Duan, Jielin;Yin, Jie;Liu, Gang;Cao, Zhong;...

期刊： Amino Acids,2021年53(2):319-319 ISSN：0939-4451

通讯作者： Xiong, Xia

作者机构： [Liu, Gang; Yin, Jie; Ren, Wenkai; Duan, Jielin; Chen, Shuai; Xiong, Xia; Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Minist Agr,Inst Subtrop Agr,Hunan Prov Engn Res C, Changsha 410125, Hunan, Peoples R China.;[Liu, Gang; Cao, Zhong] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410004, Peoples R China.;[Hou, Yongqing; Wu, Guoyao; Yin, Yulong] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Xiong, Xia] C;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Minist Agr,Inst Subtrop Agr,Hunan Prov Engn Res C, Changsha 410125, Hunan, Peoples R China.

摘要： In the published article, the images for Actin in Figs. 4a, 5a, 6a, and 6c were incorrectly presented.

语种： 英文

作者： 李雪妮;史宇涛;杜林晓;吕阳;马志鹏;...

期刊： 中国畜牧兽医,2019年46(4):1151-1157 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉 430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心 ,武汉430023;[杜林晓; 侯永清; 吕阳; 王蕾; 马志鹏; 易丹; 李雪妮; 赵迪; 史宇涛; 吴涛] 武汉轻工大学

关键词： 耐热肠毒素(STa);重组大肠杆菌;仔猪;炎症

摘要： 本试验旨在研究表达Ⅰ型耐热肠毒素(STa)的重组大肠杆菌诱发7日龄仔猪小肠炎症反应.选取24头7日龄健康仔猪,随机分为4个处理组:对照组(人工乳)、STa组(人工乳+2×109 CFU重组菌LMG194-pBAD-STa)、LMG194组(人工乳+2×109 CFU大肠杆菌LMG194)和K88组(人工乳+2×109 CFU大肠杆菌K88),每组6头.试验第5天进行攻毒,第7天屠宰取样,观察空肠组织形态学变化,并检测血清中炎性细胞因子含量及空肠免疫相关基因的相对表达量.结果显示,与对照组相比,S T a与K88组仔猪空肠绒毛明显萎缩变短,有明显损伤甚至脱落;STa、LMG194及K88组血清中IL-10、IL-8和IL-6浓度均显著升高(P<0.05),LMG194和K88组血液和肠道iFABP浓度均显著降低(P<0.05),STa和K88组血清中TNF-α浓度显著降低、NF-κB浓度显著升高,LMG194组NF-κB浓度显著降低、TNF-α浓度显著升高(P<0.05);STa和LMG194组CCL2和CXCL9基因表达水平显著上调(P<0.05),V N N1基因表达水平显著下调(P<0.05),STa和LMG194组IFN-γ组基因水平分别显著上升和下降(P<0.05);K88组CXCL9和IFN-γ基因表达水平显著下调(P<0.05),VNN1基因表达水平显著上调(P>0.05),CCL2基因表达水平无明显差异(P>0.05).以上结果表明,表达Ⅰ型耐热肠毒素STa的重组大肠杆菌几乎具有与K 88一样的毒性,可导致7日龄仔猪小肠黏膜受损,诱发严重的炎症反应,导致仔猪腹泻,可作为仔猪腹泻模型的候选菌株.

语种： 中文

作者： 宋转;赵广宇;董毅;纪昌正;李思源;...

期刊： 中国畜牧兽医,2019年46(3):684-689 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉 430023;[赵广宇; 董毅; 侯永清; 王蕾; 李思源; 易丹; 宋转; 纪昌正] 武汉轻工大学

关键词： 复合植物精油;断奶仔猪;脂多糖;肠黏膜;肠道形态结构;抗氧化能力

摘要： 为研究复合植物精油(OCT)对脂多糖(LPS)刺激断奶仔猪肠道结构和抗氧化能力的影响,本试验选取28日龄左右健康的杜×长×大三元杂交仔猪24头,按照体重相近原则随机分为3个处理组:对照组、LPS组和OCT组.对照组和LPS组仔猪饲喂基础日粮,OCT组仔猪饲喂基础日粮+50 mg/kg OCT.试验期21 d.于试验第21天,LPS组和OCT组仔猪注射LPS(100μg/(kg·BW)),对照组仔猪注射等量生理盐水.LPS或生理盐水注射后3 h采血;6 h后,屠宰全部仔猪取肠道样品测定有关指标.结果表明,与对照组相比,LPS组仔猪空肠绒毛高度/隐窝深度有降低趋势(P>0.05),回肠绒毛高度及绒毛高度/隐窝深度显著降低(P<0.05);血浆过氧化氢酶(CAT)活性、血浆和空肠超氧化物歧化酶(SOD)活性显著降低(P<0.05),空肠诱导型一氧化氮合酶(iNOS)活性、血浆和回肠H 2 O2含量显著上升(P<0.05).与LPS组相比,OCT组仔猪空肠绒毛高度、空肠绒毛高度/隐窝深度和回肠绒毛高度显著提高(P<0.05),回肠绒毛高度/隐窝深度有提高趋势(P>0.05);血浆CAT、空肠SOD活性显著提高(P<0.05),血浆和回肠H 2 O2含量显著下降(P<0.05).以上结果表明,日粮中添加50 mg/kg OCT可以在一定程度上缓解由LPS刺激引起的仔猪氧化应激,改善仔猪肠道结构.

语种： 中文

作者： 王蕾;易丹;吴涛;侯永清

期刊： 中国畜牧杂志,2019年55(8):6-12 ISSN：0258-7033

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,湖北武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉 430023;[侯永清; 王蕾; 易丹; 吴涛] 武汉轻工大学

关键词： 仔猪;肠道损伤;模型

摘要： 肠道不仅是所有营养物质消化吸收的最终场所,也是动物体内最大的免疫器官,是机体防御体系的第一道屏障,维护动物肠道健康对保障养猪生产至关重要。肠道是机体应激反应的中心器官,各种应激因素致使肠道结构损伤与功能紊乱,从而导致仔猪健康不良与生长受阻。因此,建立稳定可靠的肠道损伤模型是研究仔猪肠道损伤机制及营养干预的关键,本文主要介绍了本实验室成功建立的几种仔猪肠道损伤模型,筛选了几种肠道功能紊乱的标志性分子,为猪和人类的肠道功能障碍研究及营养干预提供参考。

语种： 中文

作者： 余魁;张越;李思源;纪昌正;董毅;...

期刊： 中国畜牧兽医,2019年46(5):1362-1369 ISSN：1671-7236

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;[赵广宇; 余魁; 董毅; 侯永清; 王蕾; 张越; 李思源; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;血浆抗氧化;肠黏膜形态;肠黏膜生长

摘要： 试验研究了日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响。选取30头体重相近的21日龄健康断奶仔猪随机分为3组(对照组、试验Ⅰ组和试验Ⅱ组),每组10个重复,每个重复1头猪。对照组饲喂基础日粮,试验Ⅰ、Ⅱ组在基础日粮的基础上分别添加2.0×10^6、2.0×10^7 CFU/g的凝结芽孢杆菌。正试期21 d,试验结束时前腔静脉采血后屠宰取样。结果显示:与对照组相比,试验Ⅰ和Ⅱ组断奶仔猪血浆超氧化物歧化酶(SOD)活性、空肠和回肠绒毛高度与隐窝深度的比值、回肠总蛋白(TP)含量及RNA与DNA的比值均显著提高(P<0.05);试验Ⅱ组仔猪血浆丙二醛(MDA)含量显著降低(P<0.05);试验Ⅰ组空肠TP含量及TP/DNA显著提高(P<0.05)。在本试验条件下,日粮中添加凝结芽孢杆菌可以提高断奶仔猪的抗氧化能力,促进仔猪肠道黏膜细胞的增殖与更新,从而改善了肠道黏膜形态结构。

语种： 中文

作者： Wu, Zhenlong;Hou, Yongqing;Dai, Zhaolai;Hu, Chien-An A.;Wu, Guoyao*

期刊： ANTIOXIDANTS & REDOX SIGNALING,2019年30(4):674-682 ISSN：1523-0864

通讯作者： Wu, Guoyao

作者机构： [Wu, Zhenlong; Dai, Zhaolai; Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing, Peoples R China.;[Hou, Yongqing; Wu, Guoyao; Hu, Chien-An A.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Hu, Chien-An A.] Univ New Mexico, Hlth Sci Ctr, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Wu, Guoyao] T;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

关键词： antioxidant;health;hydroxyproline;nutrition;oxidase;proline

摘要： SIGNIFICANCE: Hydroxyproline is a structurally and physiologically important imino acid in animals. It is provided from diets and endogenous synthesis, and its conversion into glycine enhances the production of glutathione, DNA, heme, and protein. Furthermore, oxidation of hydroxyproline by hydroxyproline oxidase (OH-POX) plays an important role in cell antioxidative reactions, survival, and homeostasis. Understanding the mechanisms whereby hydroxyproline participates in metabolism and cell signaling can improve the nutrition and health of animals and humans. Recent Advances: Hydroxyproline is highly abundant in milk and is utilized for renal synthesis of glycine to support neonatal growth, development, and survival. The oxidation of hydroxyproline by mitochondrial OH-POX generates reactive oxygen species (ROS). Enhanced ROS production contributes to the regulation of oxidative defense, apoptosis, angiogenesis, tumorigenesis, hypoxic responses, and cell survival in animals. CRITICAL ISSUES: Although dietary hydroxyproline enters the portal circulation, its utilization by the portal-drained viscera is unknown. Pathways for hydroxyproline metabolism and their regulation at the molecular, cellular, and whole-body levels remain to be defined. Furthermore, the mechanisms responsible for hydroxyproline-derived ROS and related metabolites to induce cell survival or apoptosis are unknown. FUTURE DIRECTIONS: Interorgan metabolism of hydroxyproline (including synthesis, catabolism, and flux) in animals must be quantified using isotope technologies. Efforts should also be directed toward studying dietary, hormonal, and epigenetic regulation of OH-POX expression at transcriptional and translational levels. Another emerging research need is to understand the roles of cellular redox and signaling networks involving both ROS and Delta(1)-pyrroline-3-hydroxy-5-carboxylate in nutrition, health, and disease.

语种： 英文

作者： Fu, Shulin;Zhao, Wenhua;Xiong, Chunhong;Guo, Ling;Guo, Jing;...

期刊： INNATE IMMUNITY,2019年25(7):420-432 ISSN：1753-4259

通讯作者： Qiu, Yinsheng;Hu, Chien-An Andy

作者机构： [Hu, Chien-An Andy; Liu, Yu; Zhao, Wenhua; Hou, Yongqing; Qiu, Yinsheng; Ye, Chun; Wu, Zhongyuan; Fu, Shulin; Xiong, Chunhong; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.

通讯机构： [Qiu, YS; Hu, CAA] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Baicalin;Haemophilus parasuis;apoptosis;vascular endothelial cells;invasion

摘要： Glässer’s disease, caused by Haemophilus parasuis, is a chronic disease related to an inflammatory immune response. Baicalin exerts important biological functions. In this study, we explored the protective efficacy of treatment with baicalin and the potential mechanism of activation of the MAPK signaling pathway in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis. H. parasuis stimulated expression of receptor for advanced glycation end products, induced a significant increase in the level of protein kinase-α and protein kinase-δ phosphorylation, and significantly up-regulated ERK, c-Jun N-terminal kinase, and p38 phosphorylation in PAVECs. H. parasuis also up-regulated the levels of apoptotic genes (Bax, C-myc, and Fasl) and the expression levels of c-Jun and c-Fos, and induced S-phase arrest in PAVECs. However, treatment with baicalin inhibited expression of RAGE, suppressed H. parasuis-induced protein kinase-α and protein kinase-δ phosphorylation, reduced ERK, c-Jun N-terminal kinase, and p38 phosphorylation, down-regulated apoptotic genes (Bax, C-myc, and Fasl), attenuated phospho-c-Jun production from the extracellular to the nuclei, and reversed S-phase arrest in PAVECs. In conclusion, baicalin treatment inhibited the MAPK signaling pathway, thereby achieving its anti-inflammatory responses, which provides a new strategy to control H. parasuis infection. © The Author(s) 2019.

语种： 英文

作者： 吴梦郡;纪昌正;李思源;董毅;赵广宇;...

期刊： 中国畜牧兽医,2019年46(3):669-676 ISSN：1671-7236

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉 430023;[赵广宇; 董毅; 侯永清; 吴梦郡; 王蕾; 张越; 李思源; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;小肠功能

摘要： 本研究旨在探究日粮中添加凝结芽孢杆菌（BC）对断奶仔猪小肠功能的影响。试验选取平均体重为（6.5±0.21） kg的21日龄三元杂交（杜×长×大）断奶仔猪24头,随机分为3组,每组8个重复,每个重复1头猪。对照组饲喂基础日粮,BCⅠ、BCⅡ组分别在基础日粮中添加2×106、2×107 CFU/g凝结芽孢杆菌。于试验第21天,所有仔猪灌服D-木糖（0.1 g/（kg·BW））,1 h后采血,屠宰,取回肠黏膜样品及回肠、结肠食糜。结果表明:与对照组相比,BCⅠ、BCⅡ组血浆二胺氧化酶（DAO）活力显著降低（P<0.05）,BCⅠ组血浆D-木糖含量显著增加（P<0.05）;BCⅠ、BCⅡ组回肠谷胱甘肽过氧化物酶（GSH-Px）的活力显著增加（P<0.05）,BCⅡ组回肠谷胱甘肽（GSH）含量增加（P>0.05）,BCⅠ组回肠丙二醛（MDA）含量显著降低（P<0.05）;BCⅠ组回肠黏膜水通道蛋白3（AQP3）、碱性氨基酸转运载体(b0,+AT)、钠葡萄糖共转运载体1（SGLT-1）的mRNA表达量均显著增加（P<0.05）,BCⅡ组回肠黏膜AQP3、AQP10、内向整流钾离子通道13（KCNJ13）、b0,+AT、SGLT-1的mRNA表达量均显著增加（P<0.05）;BCⅠ组回肠及结肠肠球菌、双歧杆菌数量均显著升高（P<0.05）,BCⅡ组回肠肠球菌及结肠肠球菌及双歧杆菌数量均显著升高（P<0.05）。综合试验结果,日粮中添加凝结芽孢杆菌可提高断奶仔猪小肠道吸收转运能力及抗氧化能力,改善断奶仔猪小肠内菌群结构。

语种： 中文

作者： 吴梦郡;李思源;纪昌正;余魁;董毅;...

期刊： 中国畜牧兽医,2019年46(2):424-432 ISSN：1671-7236

作者机构： [赵广宇; 余魁; 董毅; 侯永清; 吴梦郡; 李思源; 赵迪; 吴涛; 纪昌正] 武汉轻工大学

关键词： 耐热肠毒素(STa);重组大肠杆菌;仔猪;肠道

摘要： 试验旨在研究表达Ⅰ型耐热肠毒素(S T a)的重组大肠杆菌对7日龄仔猪小肠功能的影响.选取24头7日龄仔猪,随机分为4个处理组,分别为对照组、STa组、LMG194组和K88组.整个试验期间均以人工乳饲喂,预饲期4 d,试验第5天进行攻毒,STa组、LMG194组及K88组分别在早晚两次口服灌喂2×109 CFU大肠杆菌LMG194-pBAD-STa、LMG194和K88,对照组灌服等量生理盐水,试验第7天早上各组灌服D-木糖并采血,屠宰并取空肠、回肠组织样品,测量仔猪血常规指标、血浆D-木糖含量及二胺氧化酶(DAO)活力,以及吸收与转运相关基因与蛋白表达量.结果显示,与对照组相比,STa组仔猪血液中中性粒细胞、中性粒细胞比率显著降低(P<0.05),淋巴细胞比率显著升高(P<0.05),LMG194组与K88组仔猪血液中中性粒细胞比率显著降低(P<0.05),淋巴细胞比率显著升高(P<0.05);STa组与K88组仔猪血浆D-木糖含量显著降低(P<0.05);STa组、LMG194组及K88组血浆DAO活力显著升高(P<0.05);STa组与LMG194组空肠AQP8、AQP10、KCN J13和b0,+A T基因表达量均显著降低(P<0.05),K88组空肠AQP8、AQP10基因表达量显著升高(P<0.05);STa组回肠AQP8基因表达量显著降低(P<0.05),AQP10、SGLT-1基因表达量显著升高(P<0.05),LMG194组、K88组AQP8基因表达量显著升高(P<0.05),K88组AQP10、KCNJ13、b0,+AT、SGLT-1基因表达量显著降低(P<0.05);STa组、LMG194组及K88组空肠HSP70蛋白的表达量显著升高(P<0.05).以上结果表明,表达Ⅰ型耐热肠毒素的重组大肠杆菌可导致7日龄仔猪小肠产生炎症,吸收与转运能力下降.

语种： 中文

作者： Ye, Chun;Li, Ruizhi;Xu, Lei;Qiu, Yinsheng*;Fu, Shulin;...

期刊： BMC Veterinary Research,2019年15(1):1-11 ISSN：1746-6148

通讯作者： Qiu, Yinsheng

作者机构： [Ye, Chun; Li, Ruizhi; Xu, Lei; Qiu, Yinsheng; Fu, Shulin; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China;[Ye, Chun; Qiu, Yinsheng; Fu, Shulin; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA

通讯机构： [Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China. Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.

关键词： Apoptosis;Baicalin;Glasser's disease;Haemophilus parasuis;PKC-MAPK

摘要： BACKGROUND: Haemophilus parasuis (HPS) is the causative agent of Glasser's disease, characterized by arthritis, fibrinous polyserositis and meningitis, and resulting in worldwide economic losses in the swine industry. Baicalin (BA), a commonly used traditional Chinese medication, has been shown to possess a series of activities, such as anti-bacterial, anti-viral, anti-tumor, anti-oxidant and anti-inflammatory activities. However, whether BA has anti-apoptotic effects following HPS infection is unclear. Here, we investigated the anti-apoptotic effects and mechanisms of BA in HPS-induced apoptosis via the protein kinase C (PKC)-mitogen-activated protein kinase (MAPK) pathway in piglet's mononuclear phagocytes (PMNP). RESULTS: Our data demonstrated that HPS could induce reactive oxygen species (ROS) production, arrest the cell cycle and promote apoptosis via the PKC-MAPK signaling pathway in PMNP. Moreover, when BA was administered, we observed a reduction in ROS production, suppression of cleavage of caspase-3 in inducing apoptosis, and inhibition of activation of the PKC-MAPK signaling pathway for down-regulating p-JNK, p-p38, p-ERK, p-PKC-alpha and PKC-delta in PMNP triggered by HPS. CONCLUSIONS: Our data strongly suggest that BA can reverse the apoptosis initiated by HPS through regulating the PKC-MAPK signaling pathway, which represents a promising therapeutic agent in the treatment of HPS infection.

语种： 英文

作者： Fu, Shulin;Zhuang, Feng;Guo, Ling;Qiu, Yinsheng*;Xiong, Jianglin;...

期刊： International Journal of Molecular Sciences,2019年20(10):2390 ISSN：1661-6596

通讯作者： Qiu, Yinsheng

作者机构： [Hu, Chien-An Andy; Liu, Yu; Xiong, Jianglin; Zhuang, Feng; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Xiong, Jianglin; Zhuang, Feng; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.

通讯机构： [Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.

关键词： ABC transporter;aluminum;baicalin;genomic DNA;hydrogenase;nicotinamide nucleotide adenylyltransferase;phosphotransferase;aluminum;antiinfective agent;baicalin;flavonoid;animal experiment;antimicrobial activity;Article;binding site;bioinformatics;biosynthesis;cell adhesion;chemotaxis;controlled study;diarrhea;discriminant analysis;DNA extraction;DNA library;DNA purification;DNA sequence;drug efficacy;enzyme activity;feces analysis;gene expression;gene ontology;metagenomics;microbial community;microbiome;nonhuman;phylogeny;principal component analysis;Scutellaria baicalensis;sequence alignment;sequence analysis;animal;chemistry;diarrhea;drug effect;feces;intestine flora;microbiology;pig;swine disease;veterinary medicine;Aluminum;Animals;Anti-Infective Agents;Diarrhea;Feces;Flavonoids;Gastrointestinal Microbiome;Swine;Swine Diseases

摘要： The gut microbiome has important effects on gastrointestinal diseases. Diarrhea attenuation functions of baicalin (BA) is not clear. Baicalin–aluminum complexes (BBA) were synthesized from BA, but the BBA’s efficacy on the diarrhea of piglets and the gut microbiomes have not been explored and the mechanism remains unclear. This study has explored whether BBA could modulate the composition of the gut microbiomes of piglets during diarrhea. The results showed that the diarrhea rate reduced significantly after treatment with BBA. BBA altered the overall structure of the gut microbiomes. In addition, the Gene Ontology (GO) enrichment analysis indicated that the functional differentially expressed genes, which were involved in the top 30 GO enrichments, were associated with hydrogenase (acceptor) activity, nicotinamide-nucleotide adenylyltransferase activity, and isocitrate lyase activity, belong to the molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that flagellar assembly, bacterial chemotaxis, lipopolysaccharide biosynthesis, ATP-binding cassette transporters (ABC) transporters, biosynthesis of amino acids, and phosphotransferase system (PTS) were the most enriched during BBA treatment process. Taken together, our results first demonstrated that BBA treatment could modulate the gut microbiomes composition of piglets with diarrhea, which may provide new potential insights on the mechanisms of gut microbiomes associated underlying the antimicrobial efficacy of BBA. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhang, Jing;Xu, Xin;Zhu, Huiling;Wang, Yang;Hou, Yongqing;...

期刊： INNATE IMMUNITY,2019年25(1):60-72 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Wang, Yang; Zhang, Jing; Liu, Yulan; Hou, Yongqing; Xu, Xin; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Fish oil;inflammation;lipopolysaccharide;liver;RNA sequencing;pig

摘要： Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury. © The Author(s) 2018.

语种： 英文

作者： Hou, Yongqing;Li, Xilong;Dai, Zhaolai;Wu, Zhenlong;Bazer, Fuller W;...

期刊： Methods in Molecular Biology,2018年1694:105-115 ISSN：1064-3745

通讯作者： Wu, G.

作者机构： [Hou, Yongqing] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China;[Li, Xilong] Institute of Feed Science, The Chinese Academy of Agricultural Sciences, Beijing, 100081, China;[Dai, Zhaolai; Wu, Zhenlong] State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China;[Bazer, Fuller W] Department of Animal Science, Texas A&M University, 2471 TAMU, RM 133 Kleberg, College Station, TX, 77843-2471, USA;[Wu, Guoyao] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China. g-wu@tamu.edu

通讯机构： Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, China

关键词： *Cysteine;*Glutathione;*HPLC;*Polyamines;*o-Phthalaldehyde

摘要： Glutathione (GSH) forms conjugates with polyamines in prokaryotes and eukaryotes. There is also evidence suggesting cross-talk between GSH and polyamines to regulate cellular homeostasis and function, particularly under the conditions of oxidative stress. Because of its versatile roles in cell metabolism and function, a number of high performance liquid chromatography (HPLC) methods have been developed for glutathione analysis. Here, we describe our rapid and sensitive method for the analysis of GSH and the oxidized form of glutathione (GS-SG) in animal tissues and cells by HPLC involving pre-column derivatization with o-phthalaldehyde (OPA). OPA reacts very rapidly (within 1 min) with S-carboxymethylglutathione at room temperatures (e.g., 20–25 °C) in an autosampler, and their derivatives are immediately injected into the HPLC column without any need for extraction. This method requires two simple steps (a total of 15 min) before samples are loaded into the autosampler: (a) the conversion of GS-SG into GSH by 2-mercaptoethanol; and (b) the oxidation of GSH by iodoacetic acid to yield S-carboxymethyl-glutathione. The autosampler is programmed to mix S-carboxymethyl-glutathione with OPA for 1 min to generate a highly fluorescent derivative for HPLC separation and detection (excitation wavelength 340 nm and emission wavelength 450 nm). The detection limit for GSH and GS-SG is 15 pmol/ml or 375 fmol/ injection. The total time for chromatographic separation (including column regeneration) is 16 min for each sample. Our routine HPLC technique is applicable for analyses of cysteine and cystine, as well as polyamines and GSH-polyamine conjugates in biological samples. © Springer Science+Business Media LLC 2018.

语种： 英文

作者： Hou, Yongqing;Wu, Guoyao*

期刊： Amino Acids,2018年50(11):1497-1510 ISSN：0939-4451

通讯作者： Wu, Guoyao

作者机构： [Hou, Yongqing; Wu, Guoyao] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Wu, Guoyao] W;[Wu, Guoyao] T;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

关键词： Amino acids;Function;Growth;Physiology;Requirement

摘要： l-Glutamate (Glu) has traditionally not been considered as a nutrient needed in diets for humans and other animals (including swine) due to the unsubstantiated assumption that animals can synthesize sufficient amounts of Glu to meet their needs. The lack of knowledge about Glu nutrition has contributed to suboptimal efficiency of global livestock production. Over the past 25 years, there has been growing interest in Glu metabolism in the pig, which is an agriculturally important species and also a useful model for studying human biology. Because of analytical advances in its analysis, Glu is now known to be a highly abundant free amino acid in milk and intracellular fluid, a major constituent of food and tissue proteins, and a key regulator of gene expression, cell signaling, and anti-oxidative reactions. Emerging evidence shows that dietary supplementation with 2% Glu maintains gut health and prevents intestinal dysfunction in weanling piglets, while enhancing their growth performance and survival. In addition, the inclusion of 2% Glu is required for dietary arginine to maximize the growth performance and feed efficiency in growing pigs, whereas dietary supplementation with 2% Glu reduces the loss of skeletal muscle mass in endotoxin-challenged pigs. Furthermore, supplementing 2% Glu to a corn- and soybean-meal-based diet promotes milk production by lactating sows. Thus, an adequate amount of dietary Glu as a quantitatively major nutrient is necessary to support maximum growth, development, and production performance of swine. These results also have important implications for improving the nutrition and health of humans and other animals.

语种： 英文

作者： Wu, Tao;Li, Kang;Yi, Dan;Wang, Lei;Zhao, Di;...

期刊： International Journal of Molecular Sciences,2018年19(10):3277- ISSN：1661-6596

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Li, Kang; Lv, Yang; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.

关键词： intestinal morphology and function;glycogen and fat metabolism;trihexanoin;weaned piglets

摘要： Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: The control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate; increased the concentrations of LDL, HDL and total protein in plasma; decreased cholesterol concentrations and glutamyl transpeptidase activity in plasma; improved intestinal morphologic structure; altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism, mucosal barrier function, antioxidant capacity and water transport capacity; and altered the community of intestinal microflora. These results indicate that dietary trihexanoin supplementation could reduce diarrhea, regulate carbohydrate and fat metabolism, exert beneficial effects on the intestinal mucosal barrier, protect the intestinal mucosa from injuries, improve intestinal transport and absorption, and enhance antioxidant capacity. In conclusion, dietary supplementation with 0.5% trihexanoin improves the intestinal function and health of weaned piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Fu, Shulin;Liu, Huashan;Xu, Lei;Qiu, Yinsheng*;Liu, Yu;...

期刊： Scientific Reports,2018年8(1):807 ISSN：2045-2322

通讯作者： Qiu, Yinsheng

作者机构： [Hu, Chien-An Andy; Liu, Huashan; Liu, Yu; Hou, Yongqing; Xu, Lei; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.

通讯机构： [Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.

摘要： <jats:title>Abstract</jats:title><jats:p><jats:italic>Haemophilus parasuis (H. parasuis)</jats:italic> can cause vascular inflammatory injury, but the molecular basis of this effect remains unclear. In this study,we investigated the effect of the anti-inflammatory, anti-microbial and anti-oxidant agent, baicalin, on the nuclear factor (NF)-κB and NLRP3 inflammasome signaling pathway in pig primary aortic vascular endothelial cells. Activation of the NF-κB and NLRP3 inflammasome signaling pathway was induced in <jats:italic>H. parasuis</jats:italic>-infected cells. However, baicalin reduced the production of reactive oxygen species, apoptosis, and activation of the NF-κB and NLRP3 inflammasome signaling pathway in infected cells. These results revealed that baicalin can inhibit <jats:italic>H. parasuis</jats:italic>-induced inflammatory responses in porcine aortic vascular endothelial cells, and may thus offer a novel strategy for controlling and treating <jats:italic>H. parasuis</jats:italic> infection. Furthermore, the results suggest that piglet primary aortic vascular endothelial cells may provide an experimental model for future studies of <jats:italic>H. parasuis</jats:italic> infection.</jats:p>

语种： 英文

作者： Yang, Xiaojian;Yin, Fugui;Yang, Yuhui;Lepp, Dion;Yu, Hai;...

期刊： Scientific Reports,2018年8(1):4940 ISSN：2045-2322

通讯作者： Gong, Joshua

作者机构： [Yang, Xiaojian; Lepp, Dion; Gong, Joshua; Yin, Fugui; Yu, Hai] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.;[Yin, Fugui; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Yang, Yuhui] Jiangnan Univ, Sch Food Sci & Technol, Lab Food Nutr & Funct Factors, Wuxi 214122, Jiangsu, Peoples R China.;[Yin, Yulong; Ruan, Zheng] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China.;[Yang, Chengbo] Univ Manitoba, Dept Anim Sci, Winnipeg, MB R3T 2N2, Canada.

通讯机构： [Gong, Joshua] A;Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.

摘要： <jats:title>Abstract</jats:title><jats:p>Butyrate can modulate the immune response and energy expenditure of animals and enhance intestinal health. The present study investigated changes in the intestinal microbiota composition and serum metabolites of young broilers in response to 3,000 ppm butyrate in the form of butyrate glycerides (BG) via pyrosequencing of bacterial 16S rRNA genes and nuclear magnetic resonance (NMR). The dietary treatment did not affect the alpha diversity of intestinal microbiota, but altered its composition. Thirty-nine key operational taxonomic units (OTUs) in differentiating cecal microbiota community structures between BG treated and untreated chickens were also identified. <jats:italic>Bifidobacterium</jats:italic> was, in particular, affected by the dietary treatment significantly, showing an increase in not only the abundance (approximately 3 fold, <jats:italic>P</jats:italic> ≤ 0.05) but also the species diversity. The (NMR)-based analysis revealed an increase in serum concentrations of alanine, low-density and very low-density lipoproteins, and lipids (<jats:italic>P</jats:italic> ≤ 0.05) by BG. More interestingly, the dietary treatment also boosted (<jats:italic>P</jats:italic> ≤ 0.05) serum concentrations of bacterial metabolites, including choline, glycerophosphorylcholine, dimethylamine, trimethylamine, trimethylamine-N-oxide, lactate, and succinate. In conclusion, the data suggest the modulation of intestinal microbiota and serum metabolites by BG dietary treatment and potential contribution of intestinal bacteria to lipid metabolism/energy homeostasis in broilers.</jats:p>

语种： 英文

作者： Lv, Yang;Kang, Ping;Zhang, Lin;Li, Xueni;Wang, Lei;...

期刊： THAI JOURNAL OF VETERINARY MEDICINE,2018年48(2):147-156 ISSN：0125-6491

通讯作者： Wu, Tao

作者机构： [Wu, Tao; Lv, Yang; Kang, Ping; Hou, Yongqing; Wang, Lei; Li, Xueni; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.

通讯机构： [Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.

关键词： Epidermal growth factor;Intestinal histology;LPS-challenged mice;Lactobacillus acidophilus

摘要： The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named ΔMG6243-1 and ΔMG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 μl MG6243, 4) LPS-challenged + 200 μl ΔMG6243-1, 5) LPS-challenged + 200 μl ΔMG6243-1 + inducer IPTG, and 6) LPS-challenged + 200 μl ΔMG6243-2. There were ten replicates for each treatment, and each replicate had one mouse. On d 15, the LPS-challenged mice were injected intraperitoneally with LPS at 10 mg/kg BW, while the non-challenged mice were injected with 0.9% NaCl solution. Three hours after the challenge, all mice were killed. Results showed that both ΔMG6243-1 and ΔMG6243-2 could increase villus height in the duodenum, ileum and jejunum (P < 0.05). In addition, the recombinant L. acidophilus decreased the crypt depth in the duodenum and ileum (P < 0.05). These results indicated that the recombinant L. acidophilus could effectively improve the intestinal histology in LPS-challenged mice. © 2018 Chulalongkorn University Printing House.

语种： 英文

作者： Xu, Xiao;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;Liu, Congcong;...

期刊： International Journal of Molecular Sciences,2018年19(7):1980 ISSN：1661-6596

通讯作者： Liu, Yulan

作者机构： [Wu, Huanting; Liu, Congcong; Xu, Xiao; Liu, Yulan; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Dai, Bing; Liu, Xiuting] Zhe Jiang Goshine Test Technol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： adenosine triphosphate;glycine;iron;lipopolysaccharide;mammalian target of rapamycin;messenger RNA;rapamycin;sodium chloride;toll like receptor 4;zinc;AMP-activated protein kinase kinase;glycine;lipopolysaccharide;protein kinase;target of rapamycin kinase;toll like receptor 4;animal experiment;animal model;Article;colitis;controlled study;diet supplementation;Escherichia coli;gene expression;immunoblotting;inflammation;intestine injury;nonhuman;protein degradation;protein expression;real time polymerase chain reaction;RNA extraction;signal transduction;Western blotting;animal;drug effect;genetics;ileum;immunology;intestine mucosa;intraperitoneal drug administration;jejunum;metabolism;pathology;pig;weaning;Animals;Escherichia coli;Glycine;Ileum;Inflammation;Injections, Intraperitoneal;Intestinal Mucosa;Jejunum;Lipopolysaccharides;Models, Animal;Protein Kinases;RNA, Messenger;Swine;Toll-Like Receptor 4;TOR Serine-Threonine Kinases;Weaning

摘要： This study was conducted to envaluate whether glycine could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial energy status, protein synthesis, and inflammatory response via AMPK, mTOR, TLR4, and NOD signaling pathways. A total of 24 weanling piglets were randomly allotted to 1 of 4 treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 1% glycine; (4) LPS + 2% glycine. After 28 days feeding, piglets were injected intraperitoneally with saline or LPS. The pigs were slaughtered and intestinal samples were collected at 4 h postinjection. The mRNA expression of key genes in these signaling pathways was measured by real-time PCR. The protein abundance was measured by Western blot analysis. Supplementation with glycine increased jejunal villus height/crypt depth ratio. Glycine also increased the jejunal and ileal protein content, RNA/DNA ratio, and jejunal protein/DNA ratio. The activities of citroyl synthetase in ileum, and α-ketoglutarate dehydrogenase complex in jejunum, were increased in the piglets fed diets supplemented with glycine. In addition, glycine decreased the jejunal and ileal phosphorylation of AMPKα, and increased ileal phosphorylation of mTOR. Furthermore, glycine downregulated the mRNA expression of key genes in inflammatory signaling. Meanwhile, glycine increased the mRNA expression of negative regulators of inflammatory signaling. These results indicate that glycine supplementation could improve energy status and protein synthesis by regulating AMPK and mTOR signaling pathways, and relieve inflammation by inhibiting of TLR4 and NOD signaling pathways to alleviate intestinal injury in LPS-challenged piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhu, Huiling;Wang, Haibo;Wang, Shuhui;Tu, Zhixiao;Zhang, Lin;...

期刊： MOLECULAR NUTRITION & FOOD RESEARCH,2018年62(9):e1700814 ISSN：1613-4125

通讯作者： Liu, Yulan

作者机构： [Liu, Yulan; Hou, Yongqing; Wang, Haibo; Wang, Shuhui; Wang, Chunwei; Tu, Zhixiao; Zhang, Lin; Wang, Xiuying; Chen, Jie; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.

关键词： flaxseed oil;inflammation;intestinal barrier function;lipopolysaccharide;necroptosis signaling pathway;weaned pigs

摘要： Scope: Flaxseed oil is a rich source of α-linolenic acid (ALA), which is the precursor of the long-chain n-3 polyunsaturated fatty acids (PUFAs), including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). This study investigates the protective effect of flaxseed oil against intestinal injury induced by lipopolysaccharide (LPS). Materials and results: Twenty-four weaned pigs were used in a 2 × 2 factorial experiment with dietary treatment (5% corn oil vs 5% flaxseed oil) and LPS challenge (saline vs LPS). On day 21 of the experiment, pigs were administrated with LPS or saline. At 2 h and 4 h post-administration, blood samples were collected. After the blood harvest at 4 h, all piglets were slaughtered and intestinal samples were collected. Flaxseed oil supplementation led to the enrichment of ALA, EPA, and total n-3 PUFAs in intestine. Flaxseed oil improved intestinal morphology, jejunal lactase activity, and claudin-1 protein expression. Flaxseed oil downregulated the mRNA expression of intestinal necroptotic signals. Flaxseed oil also downregulated the mRNA expression of intestinal toll-like receptors 4 (TLR4) and its downstream signals myeloid differentiation factor 88 (MyD88), nuclear factor kappa B (NF−κB), and nucleotide-binding oligomerization domain proteins 1, 2 (NOD1, NOD2) and its adapter molecule, receptor-interacting protein kinase 2 (RIPK2). Conclusion: These results suggest that dietary addition of flaxseed oil enhances intestinal integrity and barrier function, which is involved in modulating necroptosis and TLR4/NOD signaling pathways. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

语种： 英文