Use of homoarginine for measuring true ileal digestibility of amino acids in food protein
作者:
Yin, Jie;Ren, Wenkai;Hou, Yongqing;Wu, Miaomiao;Xiao, Hao;...
期刊:
Amino Acids ,2015年47(9):1795-1803 ISSN:0939-4451
通讯作者:
Yin, Yulong
作者机构:
[Xiao, Hao; Yin, Jie; Ren, Wenkai; Wu, Miaomiao; Duan, Jielin; Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.;[Yin, Jie; Ren, Wenkai] Univ Chinese Acad Sci, Beijing 100039, Peoples R China.;[Hou, Yongqing; Wu, Guoyao; Yin, Yulong] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Zhao, Yurong] Univ Hunan Agr, Dept Anim Sci, Changsha 410128, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Yin, Yulong] C;Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Prov Engn Res Ctr Hlth Livestock,Minist Agr, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.
关键词:
Homoarginine;Guanidination;Amino acids;True ileal digestibility
摘要:
A useful application of homoarginine in animal nutrition is the determination of the true ileal digestibility (TID) of amino acids (AA) in swine complete diets and feed ingredients. The homoarginine method involves the conversion of dietary lysine to homoarginine in a guanidination reaction with methylisourea. Accurate determination of TID of AA, especially in heat-treated feed ingredients, is a key prerequisite for accurate diet formulation with respect to the provision of dietary AA. Thus, the aim of this review is to highlight the homoarginine methodology and its application in animal nutrition. Based on the data from published studies, the homoarginine method can be used to accurately determine the digestibility of lysine and the majority of other acid-stable AA in complete diets and feed ingredients fed to animals.
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英文
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天冬氨酸对脂多糖刺激仔猪结肠黏膜结构和屏障功能的影响
作者:
刘称;朱惠玲;刘玉兰;皮定安;冷炜博;...
期刊:
中国畜牧杂志 ,2015年51(01):38-42 ISSN:0258-7033
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[侯永清; 吴欢听; 王秀英; 冷炜博; 刘称; 皮定安; 刘玉兰; 朱惠玲] 武汉轻工大学
关键词:
天冬氨酸;脂多糖;结肠黏膜;屏障功能;仔猪
摘要:
本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激仔猪结肠黏膜结构和屏障功能的影响。试验选用24头仔猪,4个处理组分别为对照组、LPS组、0.5%ASP组、1.0%ASP组。试验第19天,分别给LPS组、0.5%ASP组、1.0%ASP组试验猪注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射后24 h屠宰,取结肠样品待测。结果表明:对照组仔猪结肠形态结构完整;注射LPS后,结肠上皮部分脱落;ASP组轻度脱落,与LPS组相比,0.5%ASP组结肠隐窝深度显著升高(P<0.05);0.5%和1.0%ASP可显著缓解LPS刺激导致的结肠杯状细胞数目的增多(P<0.05);0.5%ASP具有提高结肠中性粒细胞密度的趋势(P<0.10)。0.5%和1.0%ASP可显著缓解LPS刺激导致的结肠和血浆二胺氧化酶活性的降低(P<0.05)。以上研究结果表明,日粮添加ASP可有效阻止LPS对结肠结构和屏障功能的损伤。
语种:
中文
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L-Glutamine enhances enterocyte growth via activation of the mTOR signaling pathway independently of AMPK
作者:
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Ouyang, Wanjin;Long, Minhui;...
期刊:
Amino Acids ,2015年47(1):65-78 ISSN:0939-4451
通讯作者:
Hou, Yongqing
作者机构:
[Ouyang, Wanjin; Long, Minhui; Liu, Yulan; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词:
AMPK;IPEC-1;l-Glutamine;mTOR
摘要:
Neonates (including human infants) require l-glutamine (Gln) for optimal intestinal health. This study tested the hypothesis that Gln enhances enterocyte growth via both mammalian target of rapamycin (mTOR) and AMP-activated kinase (AMPK) signaling pathways. Intestinal porcine epithelial cells (IPEC-1) were cultured for 3 days in Gln-free Dulbecco's modified Eagle medium containing 0 or 2 mM Gln. To determine the role of mTOR and AMPK on cell growth, additional experiments were conducted where medium contained 2 mM Gln and 10 nM rapamycin (Rap, an inhibitor of mTOR) or 1 μM compound C (an inhibitor of AMPK). IPEC-1 cell growth increased with increasing concentrations of Gln from 0 to 2 mM. Compared with 0 mM Gln, 2 mM Gln increased (P < 0.05) the amounts of phosphorylated 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase (p70S6 kinase) proteins but did not affect abundances of total or phosphorylated AMPK protein. Gln also increased mRNA levels for Bcl-2, mTOR, p70S6 kinase, 4E-BP1, COX7C, ASCT2, ODC, SGLT-1, CFTR, Na+/K+-ATPase, HSP70, and ZO-1. Similarly, cells cultured with Rap and Gln exhibited higher (P < 0.05) abundances of phosphorylated 4E-BP1 and p70S6 kinase proteins than the Rap-only group, whereas abundances of phosphorylated mTOR and 4E-BP1 proteins were increased when AMPK was inhibited by compound C. Conversely, the amount of phosphorylated AMPK increased when mTOR was inhibited by Rap, suggesting a negative cross-talk between mTOR and AMPK. Collectively, these results indicate that Gln stimulates enterocyte growth by activating the mTOR signaling pathway independently of AMPK. © 2014 Springer-Verlag Wien.
语种:
英文
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Beneficial roles of dietary oleum cinnamomi in alleviating intestinal injury
作者:
Wang, Lei;Hou, Yongqing* ;Yi, Dan;Ding, Binying;Zhao, Di;...
期刊:
FRONTIERS IN BIOSCIENCE-LANDMARK ,2015年20(5):814-828 ISSN:2768-6701
通讯作者:
Hou, Yongqing
作者机构:
[Gong, Joshua; Liu, Yulan; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Ding, Binying; Zhao, Di; Yi, Dan; Wang, Zhongxing; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao; Assaad, Houssein] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Intestinal mucosa;Lipopolysaccharide;Oleum cinnamomi;Piglets;Review
摘要:
Cinnamon is a traditional herb used for treatment of many human diseases. The most important chemical compounds of the essential oil are cinnamaldehyde and eugenol. Oleum cinnamomi (OCM, cinnamon oil) is increasingly used as a feed additive to animal diets. Beneficial effects of OCM in protecting tissues from inflammation and injury by endogenous and exogenous agents (such as hydrogen peroxide and lipopolysaccharide (LPS)) may result, in part, from its action on regulating amino acid metabolism in cells to favor the synthesis of glutathione (a major low-molecular-weight antioxidant) from cysteine, glycine and glutamate. In support of this notion, results of recent studies indicate that supplementing OCM (50 mg/kg diet) to a corn- and soybean meal-based diet for piglets weaned at 21 days of age enhances intestinal anti-oxidative capacity and reduces the incidence of diarrhea. Additionally, dietary supplementation with OCM ameliorates LPS-induced mucosal barrier dysfunction and mucosal damage in the small intestine. OCM holds great promise for protecting the gut from injury under conditions of inflammation, infections, and oxidative stress. © 2015, Frontiers in Bioscience. All rights reserved.
语种:
英文
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Asparagine attenuates intestinal injury, improves energy status and inhibits AMP-activated protein kinase signalling pathways in weaned piglets challenged with Escherichia coli lipopolysaccharide
作者:
Wang, Xiuying;Liu, Yulan* ;Li, Shuang;Pi, Dingan;Zhu, Huiling;...
期刊:
British Journal of Nutrition ,2015年114(4):553-565 ISSN:0007-1145
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Leng, Weibo; Li, Shuang; Hou, Yongqing; Shi, Haifeng; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Asparagine;Intestine;Energy status;Weaned piglets;Lipopolysaccharide
摘要:
The intestine requires a high amount of energy to maintain its health and function; thus, energy deficits in intestinal mucosa may lead to intestinal damage. Asparagine (Asn) is a precursor for many other amino acids such as aspartate, glutamine and glutamate, which can be used to supply energy to enterocytes. In the present study, we hypothesise that dietary supplementation of Asn could alleviate bacterial lipopolysaccharide (LPS)-induced intestinal injury via improvement of intestinal energy status. A total of twenty-four weaned piglets were assigned to one of four treatments: (1) non-challenged control; (2) LPS+0 % Asn; (3) LPS+0.5 % Asn; (4) LPS+1.0 % Asn. On day 19, piglets were injected with LPS or saline. At 24 h post-injection, piglets were slaughtered and intestinal samples were collected. Asn supplementation improved intestinal morphology, indicated by higher villus height and villus height:crypt depth ratio, and lower crypt depth. Asn supplementation also increased the ratios of RNA:DNA and protein:DNA as well as disaccharidase activities in intestinal mucosa. In addition, Asn supplementation attenuated bacterial LPS-induced intestinal energy deficits, indicated by increased ATP and adenylate energy charge levels, and decreased AMP:ATP ratio. Moreover, Asn administration increased the activities of key enzymes involved in the tricarboxylic acid cycle, including citrate synthase, isocitrate dehydrogenase and α-ketoglutarate dehydrogenase complex. Finally, Asn administration decreased the mRNA abundance of intestinal AMP-activated protein kinase-α1 (AMPKα1), AMPKα2, silent information regulator 1 (SIRT1) and PPARγ coactivator-1α (PGC1α), and reduced intestinal AMPKα phosphorylation. Collectively, these results indicate that Asn supplementation alleviates bacterial LPS-induced intestinal injury by modulating the AMPK signalling pathway and improving energy status. Copyright © The Authors 2015.
语种:
英文
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Rapid publication-ready MS-Word tables for two-way ANOVA
作者:
Assaad, Houssein I.* ;Hou, Yongqing;Zhou, Lan;Carroll, Raymond J.;Wu, Guoyao
期刊:
SpringerPlus ,2015年4(1):1-9 ISSN:2193-1801
通讯作者:
Assaad, Houssein I.
作者机构:
[Assaad, Houssein I.; Carroll, Raymond J.; Zhou, Lan] Texas A&M Univ, Dept Stat, College Stn, TX 77843 USA.;[Assaad, Houssein I.; Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Yongqing; Wu, Guoyao] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Assaad, Houssein I.] T;Texas A&M Univ, Dept Stat, College Stn, TX 77843 USA.
关键词:
Two-way ANOVA;Multiple comparisons;Online software;Statistical analysis;Biology;Agriculture;R;Shiny
摘要:
Statistical tables are an essential component of scientific papers and reports in biomedical and agricultural sciences. Measurements in these tables are summarized as mean ± SEM for each treatment group. Results from pairwise-comparison tests are often included using letter displays, in which treatment means that are not significantly different, are followed by a common letter. However, the traditional manual processes for computation and presentation of statistically significant outcomes in MS Word tables using a letter-based algorithm are tedious and prone to errors. Using the R package ‘Shiny’, we present a web-based program freely available online, at
https://houssein-assaad.shinyapps.io/TwoWayANOVA/
. No download is required. The program is capable of rapidly generating publication-ready tables containing two-way analysis of variance (ANOVA) results. Additionally, the software can perform multiple comparisons of means using the Duncan, Student-Newman-Keuls, Tukey Kramer, Westfall, and Fisher’s least significant difference (LSD) tests. If the LSD test is selected, multiple methods (e.g., Bonferroni and Holm) are available for adjusting p-values. Significance statements resulting from all pairwise comparisons are included in the table using the popular letter display algorithm. With the application of our software, the procedures of ANOVA can be completed within seconds using a web-browser, preferably Mozilla Firefox or Google Chrome, and a few mouse clicks. To our awareness, none of the currently available commercial (e.g., Stata, SPSS and SAS) or open-source software (e.g., R and Python) can perform such a rapid task without advanced knowledge of the corresponding programming language. The new and user-friendly program described in this paper should help scientists perform statistical analysis and rapidly generate publication-ready MS-Word tables for two-way ANOVA. Our software is expected to facilitate research in agriculture, biomedicine, and other fields of life sciences.
语种:
英文
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Effects of tributyrin on intestinal energy status, antioxidative capacity and immune response to lipopolysaccharide challenge in broilers
作者:
Li, Jiaolong;Hou, Yongqing;Yi, Dan;Zhang, Jun;Wang, Lei;...
期刊:
ANIMAL BIOSCIENCE ,2015年28(12):1784-1793 ISSN:2765-0189
通讯作者:
Ding, Binying
作者机构:
[Qin, Hongyi; Hou, Yongqing; Li, Jiaolong; Wang, Lei; Ding, Binying; Yi, Dan; Zhang, Jun] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Ding, Binying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Ding, Binying] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Tributyrin;Lipopolysaccharide;Intestine;Immune Response;Broilers
摘要:
This study was carried out to investigate the effects of tributyrin (TB) on the growth performance, pro-inflammatory cytokines, intestinal morphology, energy status, disaccharidase activity, and antioxidative capacity of broilers challenged with lipopolysaccharide (LPS). A total of 160 one-day-old Cobb broilers were allocated to 1 of 4 treatments, with 4 replicated pens per treatment and 10 birds per pen. The experiment consisted of a 2×2 factorial arrangements of treatments with TB supplementation (0 or 500 mg/kg) and LPS challenge (0 or 500 μg/kg body weight [BW]). On days 22, 24, and 26 of the trial, broilers received an intraperitoneal administration of 500 μ g/kg BW LPS or saline. Dietary TB showed no effect on growth performance. However, LPS challenge decreased the average daily gain of broilers from day 22 to day 26 of the trial. Dietary TB supplementation inhibited the increase of interleukin-1β (in the jejunum and ileum), interleukin-6 (in the duodenum and jejunum), and prostaglandin E2 (in the duodenum) of LPS-challenged broilers. Similar inhibitory effects of TB in the activities of total nitric oxide synthase (in the ileum) and inducible nitric oxide synthase (in the jejunum) were also observed in birds challenged with LPS. Additionally, TB supplementation mitigated the decrease of ileal adenosine triphosphate, adenosine diphosphate and total adenine nucleotide and the reduction of jejunal catalase activity induced by LPS. Taken together, these results suggest that the TB supplementation was able to reduce the release of pro-inflammatory cytokines and improve the energy status and anti-oxidative capacity in the small intestine of LPS-challenged broilers. Copyright © 2015 by Asian-Australasian Journal of Animal Sciences.
语种:
英文
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Regulation of the intestinal barrier function by host defense peptides
作者:
Robinson, Kelsy;Deng, Zhuo;Hou, Yongqing;Zhang, Guolong*
期刊:
Frontiers in Veterinary Science ,2015年2(NOV):57 ISSN:2297-1769
通讯作者:
Zhang, Guolong
作者机构:
[Zhang, Guolong; Deng, Zhuo; Robinson, Kelsy] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Zhang, Guolong; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构:
[Zhang, Guolong] O;Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.
关键词:
barrier function;gut health;host defense peptides;innate immunity;tight junction
摘要:
Intestinal barrier function is achieved primarily through regulating the synthesis of mucins and tight junction (TJ) proteins, which are critical for maintaining optimal gut health and animal performance. An aberrant expression of TJ proteins results in increased paracellular permeability, leading to intestinal and systemic disorders. As an essential component of innate immunity, host defense peptides (HDPs) play a critical role in mucosal defense. Besides broad-spectrum antimicrobial activities, HDPs promotes inflammation resolution, endotoxin neutralization, wound healing, and the development of adaptive immune response. Accumulating evidence has also indicated an emerging role of HDPs in barrier function and intestinal homeostasis. HDP deficiency in the intestinal tract is associated with barrier dysfunction and dysbiosis. Several HDPs were recently shown to enhance mucosal barrier function by directly inducing the expression of multiple mucins and TJ proteins. Consistently, dietary supplementation of HDPs often leads to an improvement in intestinal morphology, production performance, and feed efficiency in livestock animals. This review summarizes current advances on the regulation of epithelial integrity and homeostasis by HDPs. Major signaling pathways mediating HDP-induced mucin and TJ protein synthesis are also discussed. As an alternative strategy to antibiotics, supplementation of exogenous HDPs or modulation of endogenous HDP synthesis may have potential to improve intestinal barrier function and animal health and productivity. © 2015 Robinson, Deng, Hou and Zhang.
语种:
英文
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N-acetylcysteine and intestinal health: A focus on mechanisms of its actions
作者:
Hou, Yongqing* ;Wang, Lei;Yi, Dan;Wu, Guoyao
期刊:
FRONTIERS IN BIOSCIENCE-LANDMARK ,2015年20(5):872-891 ISSN:2768-6701
通讯作者:
Hou, Yongqing
作者机构:
[Hou, Yongqing; Wang, Lei; Wu, Guoyao; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Acetic acid;Intestinal functions;Lipopolysaccharide;N-acetylcysteine;Piglets;Review
摘要:
The integrity of the intestinal epithelium ensures its normal physiological function. Consequently, damage to the mucosal epithelium can impair the absorption of nutrients, thereby reducing the growth performance and compromising the health of animals. N-acetylcysteine (NAC) is pharmaceutically available either intravenously, orally, or by inhalation for reducing endothelial dysfunction, inflammation, fibrosis, invasion, cartilage erosion, acetaminophen detoxification, and transplant prolongation. NAC is rapidly metabolized by the small intestine to produce glutathione and can not be detected in animals without supplementation. The physiologic functions and therapeutic effects of NAC are largely associated with maintaining intracellular concentrations of reduced glutathione. Results from recent studies indicate that NAC reduces inflammation, alleviates oxidative stress, improves energy status, and ameliorates tissue damage in the intestine of lipopolysaccharide-challenged piglets. Moreover, dietary supplementation with NAC ameliorates acetic acid-induced colitis in a porcine model. The effects of NAC are associated with some intestinal cell signaling pathways, such as EGFR, TLR4, apoptosis and tight junction signaling. The current review focuses on the protective effects of NAC on intestinal health and the molecular mechanisms of its action. © 2015, Frontiers in Bioscience. All rights reserved.
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英文
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The anti-inflammatory effects of acetaminophen and N-acetylcysteine through suppression of the NLRP3 inflammasome pathway in LPS-challenged piglet mononuclear phagocytes
作者:
Liu, Yu;Yao, Wenxu;Xu, Jun;Qiu, Yinsheng* ;Cao, Fangyuan;...
期刊:
INNATE IMMUNITY ,2015年21(6):587-597 ISSN:1753-4259
通讯作者:
Qiu, Yinsheng
作者机构:
[Yang, Sheng; Liu, Yu; Xu, Jun; Hou, Yongqing; Qiu, Yinsheng; Li, Sali; Cao, Fangyuan; Wu, Zhongyuan; Yao, Wenxu; Yang, Hailan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Liu, Yu; Hou, Yongqing; Qiu, Yinsheng; Wu, Zhongyuan] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Peoples R China.;[Qiu, Yinsheng] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
通讯机构:
[Qiu, Yinsheng] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
关键词:
Acetaminophen;N-acetylcysteine;anti-inflammation;NLRP3 inflammasome;caspase-1
摘要:
Acetaminophen (AAP) and N-acetylcysteine (NAC) have been found to have anti-inflammatory effects via the TLR-NF-ΰB pathway in LPS-challenged piglets. However, the action mechanisms employed by AAP and NAC have yet to be completely understood. This study investigated the anti-inflammatory effects and mechanisms of AAP and NAC on LPS-induced inflammatory responses via the NLRP3 inflammasome pathway in piglet mononuclear phagocytes. The results show that mRNA expression levels of IL-1β, IL-18 and NLRP3, as well as the protein level of cleaved caspase-1, are significantly increased after LPS challenge in vitro. LPS stimulation did not change ASC and caspase-1 mRNA levels, which were components of NLRP3 inflammasome complex. AAP (0.5-1.0 mM) and NAC (0.5-1.0 mM) used individually or in combination could down-regulate protein expression of cleaved caspase-1 and mRNA expression of IL-1β, IL-18 and NLRP3. NAC could significantly enhance the above inhibition actions of AAP. The combined use of AAP plus NAC had better inhibition action on the NLRP3 inflammasome pathway. These results indicate that the anti-inflammatory effects of AAP and NAC occur via the regulation on mRNA expression of NLRP3 and activation of caspase-1. The anti-inflammatory activity of AAP and NAC could be related to the suppression of NLRP3 inflammasome pathway under LPS stimulation. © The Author(s) 2015.
语种:
英文
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L-Arginine improves DNA synthesis in LPS-challenged enterocytes
作者:
Tan, Bi'e* ;Xiao, Hao;Xiong, Xia;Wang, Jing;Li, Guangran;...
期刊:
FRONTIERS IN BIOSCIENCE-LANDMARK ,2015年20(6):989-1003 ISSN:2768-6701
通讯作者:
Tan, Bi'e
作者机构:
[Xiao, Hao; Tan, Bi'e; Huang, Bo; Xiong, Xia; Li, Guangran; Wu, Guoyao; Wang, Jing; Yin, Yulong] Chinese Acad Sci, Hunan Prov Engn Res Ctr Healthy Livestock & Poult, Inst Subtrop Agr,Observat & Expt Stn Anim Nutr &, Key Lab Agroecol Proc Subtrop Reg,Minist Agr, Changsha 410125, Hunan, Peoples R China.;[Xiao, Hao; Huang, Bo; Li, Guangran; Wang, Jing] Univ Chinese Acad Sci, Beijing 10008, Peoples R China.;[Hou, Yongqing; Wu, Guoyao] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Hou, Yongqing; Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Tan, Bi'e] Chinese Acad Sci, Res Ctr Healthy Breeding Livestock & Poultry, Changsha 410125, Hunan, Peoples R China.
通讯机构:
[Tan, Bi'e] C;Chinese Acad Sci, Res Ctr Healthy Breeding Livestock & Poultry, Changsha 410125, Hunan, Peoples R China.
关键词:
Arginine;Cell Cycle;DNA synthesis;Intestine;Mitochondrial Function
摘要:
The neonatal small intestine is susceptible to damage by endotoxin, and this cytotoxicity may involve intracellular generation of reactive oxygen species (ROS), resulting in DNA damage and mitochondrial dysfunction. L-Arginine (Arg) confers a cytoprotective effect on lipopolysaccharide (LPS)-treated enterocytes through activation of the mammalian target of the rapamycin (mTOR) signaling pathway. Arg improves DNA synthesis and mitochondrial bioenergetics, which may also be responsible for beneficial effects of Arg on intestinal mucosal cells. In support of this notion, results of recent studies indicate that elevated Arg concentrations enhances DNA synthesis, cell-cycle progression, and mitochondrial bioenergetics in LPS-treated intestinal epithelial cells through mechanisms involving activation of the PI3K-Akt pathway. These findings provide a biochemical basis for dietary Arg supplementation to improve the regeneration and repair of the small-intestinal mucosa in both animals and humans. © 2015, Frontiers in Bioscience. All rights reserved.
语种:
英文
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N-乙酰半胱氨酸对肉鸭肝脏能量代谢状况及组织抗氧化指标的影响
作者:
谭灵琳;侯永清;易丹;廖满;王蕾;...
期刊:
中国畜牧杂志 ,2015年51(17):53-58 ISSN:0258-7033
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[丁斌鹰; 廖满; 侯永清; 谭灵琳; 王蕾; 易丹] 武汉轻工大学
关键词:
N-乙酰半胱氨酸;能量代谢;抗氧化能力;肉鸭
摘要:
为考查N-乙酰半胱氨酸(NAC)是否可以改善肉鸭肝脏的能量代谢状况及组织抗氧化能力,试验选用90只1 d樱桃谷肉鸭,随机分成3个处理组:对照组(基础日粮)、500 mg/kg NAC组(基础日粮+500 mg/kg NAC)、1 000 mg/kg NAC组(基础日粮+1 000 mg/kg NAC),每组3个重复,每个重复10只鸭。试验期为3周(1~21日龄)。结果表明:与对照组相比,日粮添加500 mg/kg NAC显著提高了血浆、肝脏的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活力及肝脏谷胱甘肽过氧化物酶(GSH-Px)的酶活力、三磷酸腺苷(ATP)、二磷酸腺苷(ADP)的含量和能荷(EC)值(P<0.05),显著降低了血浆丙二醛(MDA)和肝脏过氧化氢(H2O2)的含量及肝脏AMP/ATP值和肝脏低氧诱导因子1(HIF-1)、黄嘌呤氧化还原酶(XOR)的m RNA水平(P<0.05);1 000 mg/kg NAC组显著提高了血浆和肝脏CAT、SOD的活力及肝脏GSH-Px的酶活力、ATP的含量、EC值和AMPK-α1的m RNA水平(P<0.05),显著降低了血浆和肝脏H2O2的含量及肝脏MDA的含量、HIF-1、XOR的m RNA水平。表明NAC可以提高肉鸭的组织抗氧化能力、改善肝脏的能量代谢,这可能与NAC对AMPK-α1、HIF-1及XOR的基因表达调控有关。
语种:
中文
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Autophagy and tight junction proteins in the intestine and intestinal diseases
作者:
Chien-An A.Hu;Yongqing Hou;Dan Yi;Yinsheng Qiu;Guoyao Wu;...
期刊:
动物营养(英文) ,2015年1(3):123-127 ISSN:2405-6545
通讯作者:
Hu, C.-A.A.
作者机构:
Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, 87131, United States;[Hou Y.; Qiu Y.; Yi D.] Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China;Department of Animal Science, Texas A&M University, College Station, 77843, United States;[Kong X.; Yin Y.] Institute of Subtropical Agriculture, Chinese Academy of Engineering, Changsha, 410125, China;[Wu G.] Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China, Department of Animal Science, Texas A&M University, College Station, 77843, United States
通讯机构:
[Chien-An A. Hu] D;[Yongqing Hou] H;[Yulong Yin] I;Institute of Subtropical Agriculture, Chinese Academy of Engineering, Changsha 410125, China<&wdkj&>Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque 87131, USA<&wdkj&>Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China
关键词:
Autophagy;Claudins;IBD;Intestinal diseases;Proinflammatory cytokines;Tight junction barrier
摘要:
The intestinal epithelium (IE) forms an indispensible barrier and interface between the intestinal interstitium and the luminal environment. The IE regulates water, ion and nutrient transport while providing a barrier against toxins, pathogens (bacteria, fungi and virus) and antigens. The apical intercellular tight junctions (TJ) are responsible for the paracellular barrier function and regulate trans-epithelial flux of ions and solutes between adjacent cells. Increased intestinal permeability caused by defects in the IE TJ barrier is considered an important pathogenic factor for the development of intestinal inflammation, diarrhea and malnutrition in humans and animals. In fact, defects in the IE TJ barrier allow increased antigenic penetration, resulting in an amplified inflammatory response in inflammatory bowel disease (IBD), necrotizing enterocolitis and ischemia-reperfusion injury. Conversely, the beneficial enhancement of the intestinal TJ barrier has been shown to resolve intestinal inflammation and apoptosis in both animal models of IBD and human IBD. Autophagy (self-eating mechanism) is an intracellular lysosome-dependent degradation and recycling pathway essential for cell survival and homeostasis. Dysregulated autophagy has been shown to be directly associated with many pathological processes, including IBD. Importantly, the crosstalk between IE TJ and autophagy has been revealed recently. We showed that autophagy enhanced IE TJ barrier function by increasing transepithelial resistance and reducing the paracellular permeability of small solutes and ions, which is, in part, by targeting claudin-2, a cation-selective, pore-forming, transmembrane TJ protein, for lysosome (autophagy)-mediated degradation. Interestingly, previous studies have shown that the inflamed intestinal mucosa in patients with active IBD has increased claudin-2 expression. In addition, inflammatory cytokines (for example, tumor necrosis factor-α, interleukin-6, interleukin-13, and interleukin-17) whose levels are increased in IBD patients cause an increase in claudin-2 expression and a claudin-2-dependent increase in TJ permeability. Thus, the role of claudin-2 in intestinal pathological processes has been attributed, in part, to the increase of intestinal TJ permeability. Claudin-2 represents a new therapeutic target in treating IBD, diarrhea and malnutrition in animals and humans. © 2015
语种:
英文
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Metabolic profiles in the response to supplementation with composite antimicrobial peptides in piglets challenged with deoxynivalenol
作者:
Xiao, H.;Wu, M. M.;Shao, F. Y.;Tan, B. E.* ;Li, T. J.;...
期刊:
Journal of Animal Science ,2015年93(3):1114-1123 ISSN:1525-3163
通讯作者:
Tan, B. E.
作者机构:
[Wu, M. M.; Xiao, H.; Tan, B. E.; He, Q. H.; Li, T. J.; Yin, Y. L.; Yin, J.; Wang, J.; Ren, W. K.; Shao, F. Y.] Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg,Minist Agr,Obse, Changsha 410125, Hunan, Peoples R China.;[Wu, M. M.; Yin, J.; Wang, J.; Ren, W. K.; Shao, F. Y.] Univ Chinese Acad Sci Beijing, Beijing 100080, Peoples R China.;[Tan, B. E.] Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410000, Hunan, Peoples R China.;[Hou, Y. Q.] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
通讯机构:
[Tan, B. E.] C;Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg,Minist Agr,Obse, Changsha 410125, Hunan, Peoples R China.
关键词:
Composite antimicrobial peptide;Deoxynivalenol;Liquid chromatography tandem mass spectrometry;Metabolic profiles;Piglets;Proton nuclear magnetic resonance spectroscopy
摘要:
Deoxynivalenol (DON) causes various toxic effects in human and animals. However, our previous studies have shown that composite antimicrobial peptides (CAP) can have a protective effect in piglets challenged with DON. This study was conducted to evaluate the effect of the CAP GLAM 180# on the metabolism of piglets challenged with DON using a nuclear magnetic resonance (NMR)-based metabolomics approach. A total of 28 individually housed piglets (Duroc × Landrace × Large Yorkshire) weaned at 28 d of age were randomly assigned into 4 treatment groups (7 pigs/treatment) based on a 2 × 2 factorial arrangement that were fed, respectively, a basal diet (NC), basal diet + 0.4% CAP (basal + CAP), basal diet + 4 mg/kg DON (basal + DON), and basal diet + 4 mg/ kg DON + 0.4% CAP (DON + CAP). A 7-d adaptation period was followed by 30 d of treatment. Blood samples were then collected for metabolite analysis by proton NMR (1H-NMR) spectroscopy and liquid chromatography tandem mass spectrometry (LC-MS/MS). The combined results of1H-NMR spectroscopy and LC-MS/MS showed that DON increased (P < 0.05) the serum concentrations of low-density lipoprotein, glycoprotein, urea, trimethylamine-N-oxide (TMAO), and lactate as well as those of almost all essential AA and some nonessential AA but decreased the concentrations of high-density lipoprotein (HDL), unsaturated lipids, citrate, choline, and fumarate compared with those in NC treatment (P < 0.05). There was a significant interaction effect (P < 0.05) of supplementation with DON and CAP on some metabolites showed that the serum concentrations of HDL, unsaturated lipids, Pro, citrate, and fumarate were greater (P < 0.05) whereas those of glycoprotein, urea, TMAO, Gly, and lactate were lower in the DON + CAP treatment compared with those in the basal + DON treatment (P < 0.05). These findings indicated that DON causes disturbances in AA, lipid, and energy metabolism and that CAP could partially attenuate the above metabolic disturbances induced by DON. © 2015 American Society of Animal Science. All rights reserved.
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英文
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植物精油对断奶仔猪生长性能及血液生化指标的影响
作者:
方秋红;侯永清;赵迪;汪中兴;王蕾;...
期刊:
饲料工业 ,2015年35(17):44-47 ISSN:1001-991X
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉420023;[肖航; 侯永清; 王蕾; 陈振强; 汪中兴; 方秋红; 易丹; 赵迪] 武汉轻工大学
会议名称:
中国畜牧兽医学会动物营养分会第七届中国猪营养学术研讨会
会议时间:
2015-10-01
会议地点:
重庆
会议论文集名称:
中国畜牧兽医学会动物营养分会第七届中国猪营养学术研讨会论文集
关键词:
最小抑菌浓度;肉桂油;断奶仔猪;生长性能;血液生化指标
摘要:
本试验旨在筛选出抑菌效果最佳的植物精油并通过动物饲养实验研究该植物精油对断奶仔猪生长性能,血液生化指标的影响,为开发绿色饲料添加剂建立工作基础和提供理论依据.试验通过微量肉汤稀释法和刃天青指示剂测定肉桂油、牛至油、百里香油、茶树油的最小抑菌浓度(MIC),选出抑菌能力最强的植物精油,然后挑选16头体重相近,健康的21日龄"杜×长×大"三元杂交仔猪,分为2个处理组(对照组和肉桂油组),每个处理8个重复.对照组饲喂基础饲粮,肉桂油组饲喂含50 mg/kg肉桂油的基础饲粮.试验期为21d,于试验第21d,前腔静脉采血,分离血浆.采完血之后屠宰,取肝脏组织待测.(结果)结果表明:肉桂油对大肠杆菌和沙门氏菌的抑菌能力最强(MIC为800 μL/L).与对照组相比,添加50 mg/kg肉桂油能显著提高仔猪血液的总蛋白(P<0.05)和白蛋白(P<0.05)水平,红细胞比容提高了11.74% (P=0.082),谷胱甘肽过氧化物酶的活力提高了13.66% (P=0.085).由此可见:肉桂油有较强的抑菌能力,在饲粮中添加50 mg/kg的肉桂油可在一定程度上改善断奶仔猪营养状况,并有提高仔猪抗氧化能力的趋势.
语种:
中文
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Dietary essentiality of “nutritionally non-essential amino acids” for animals and humans
作者:
Hou, Yongqing;Yin, Yulong;Wu, Guoyao*
期刊:
Experimental Biology and Medicine ,2015年240(8):997-1007 ISSN:1535-3702
通讯作者:
Wu, Guoyao
作者机构:
[Hou, Yongqing; Wu, Guoyao; Yin, Yulong] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Wu, Guoyao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Amino acids;animals;humans;nutrition;requirement
摘要:
Based on growth or nitrogen balance, amino acids (AA) had traditionally been classified as nutritionally essential (indispensable) or non-essential (dispensable) for animals and humans. Nutritionally essential AA (EAA) are defined as either those AA whose carbon skeletons cannot be synthesized de novo in animal cells or those that normally are insufficiently synthesized de novo by the animal organism relative to its needs for maintenance, growth, development, and health and which must be provided in the diet to meet requirements. In contrast, nutritionally non-essential AA (NEAA) are those AA which can be synthesized de novo in adequate amounts by the animal organism to meet requirements for maintenance, growth, development, and health and, therefore, need not be provided in the diet. Although EAA and NEAA had been described for over a century, there are no compelling data to substantiate the assumption that NEAA are synthesized sufficiently in animals and humans to meet the needs for maximal growth and optimal health. NEAA play important roles in regulating gene expression, cell signaling pathways, digestion and absorption of dietary nutrients, DNA and protein synthesis, proteolysis, metabolism of glucose and lipids, endocrine status, men and women fertility, acid–base balance, antioxidative responses, detoxification of xenobiotics and endogenous metabolites, neurotransmission, and immunity. Emerging evidence indicates dietary essentiality of “nutritionally non-essential amino acids” for animals and humans to achieve their full genetic potential for growth, development, reproduction, lactation, and resistance to metabolic and infectious diseases. This concept represents a new paradigm shift in protein nutrition to guide the feeding of mammals (including livestock), poultry, and fish. © 2015, © 2015 by the Society for Experimental Biology and Medicine.
语种:
英文
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Technical note: Isolation and characterization of porcine mammary epithelial cells,
作者:
Dahanayaka, S.;Rezaei, R.;Porter, W. W.;Johnson, G. A.;Burghardt, R. C.;...
期刊:
Journal of Animal Science ,2015年93(11):5186-5193 ISSN:1525-3163
通讯作者:
Wu, G.
作者机构:
[Hou, Y. Q.; Wu, G.; Bazer, F. W.; Rezaei, R.; Dahanayaka, S.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Porter, W. W.; Burghardt, R. C.; Johnson, G. A.] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.;[Hou, Y. Q.; Wu, G.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, G.; Wu, Z. L.] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构:
[Wu, G.] T;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
关键词:
beta-casein;characterization;isolation;lactation;mammary epithelial cells;swine
摘要:
Within the mammary gland, functional synthesis of milk is performed by its epithelial (alveolar) cells. The availability of a stable mammary epithelial cell line is essential for biochemical studies to elucidate cellular and molecular mechanisms responsible for nutritional regulation of lactation. Therefore, porcine mammary epithelial cells (PMEC) were isolated from mammary glands of a 9-mo-old nonpregnant and nonlactating gilt and cultured to establish a nonimmortalized cell line. These cells were characterized by expression of cytokeratin-18 (an intermediate filament specific for epithelial cells), β-casein (a specific marker for mammary epithelial cells), and α-lactalbumin. In culture, the PMEC doubled in number every 24 h and maintained a cobblestone morphology, typical for cultured epithelial cells, for at least 15 passages. Addition of 0.2 to 2 μg/mL prolactin to culture medium for 3 d induced the production of β-casein and α-lactalbumin by PMEC in a dose-dependent manner. Thus, we have successfully developed a useful PMEC line for future studies of cellular and molecular regulation of milk synthesis by mammary epithelial cells of the sow. © 2015 American Society of Animal Science. All rights reserved.
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英文
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Technical note: Isolation and characterization of ovine brown adipocyte precursor cells,
作者:
Ma, X.;Hou, Y. Q.;Dahanayaka, S.;Satterfield, M. C.;Burghardt, R. C.;...
期刊:
Journal of Animal Science ,2015年93(5):2094-2099 ISSN:1525-3163
通讯作者:
Wu, G.
作者机构:
[Wu, G.; Ma, X.] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Hou, Y. Q.; Wu, G.; Bazer, F. W.; Burghardt, R. C.; Dahanayaka, S.; Satterfield, M. C.; Ma, X.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Y. Q.; Wu, G.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Wu, G.] C;China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
关键词:
brown adipocyte precursor cells;characterization;isolation;ovine;UCP-1
摘要:
Brown adipose tissue (BAT) plays a critical role in regulating body temperature in newborn lambs. Availability of a stable BAT cell line would be invaluable for biochemical studies to elucidate cellular and molecular mechanisms responsible for nutritional regulation of fetal BAT growth and development. Ovine brown adipocyte precursor cells (BAPC) were isolated from fetal lambs at d 90 of gestation and cultured to establish a stable cell line. These cells were characterized by adipogenic differentiation and expression of a hallmark gene, uncoupling protein 1 (UCP1). The BAPC doubled every 24 h. After a 9-d induction with a serum-free Dulbecco’s modified Eagle Ham/F12 medium, BAPC differentiated into brown adipocytes with large lipid droplets. The differentiation mediuminduced expression of UCP1 mRNA and protein in BAPC. Furthermore, after BAPC were passaged 30 times, they maintained similar cell morphology, the potential for adipogenic differentiation, and the ability to express UCP1. Taken together, we have established a stable ovine BAPC cell line for studying nutritional regulation of BAT growth and development in the fetus. © 2015 American Society of Animal Science. All rights reserved.
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英文
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Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets
作者:
Xiong, X.;Yang, H. S.;Wang, X. C.;Hu, Q.;Liu, C. X.* ;...
期刊:
Journal of Animal Science ,2015年93(3):1089-1097 ISSN:1525-3163
通讯作者:
Liu, C. X.
作者机构:
[Yang, H. S.; Wu, X.; Deng, D.; Xiao, D. F.; Wang, X. C.; Yin, Y. L.; Xiong, X.] Chinese Acad Sci, Inst Subtrop Agr,Minist Agr, Res Ctr Hlth Breeding Livestock & Poultry,Sci Obs, Hunan Engn & Res Ctr Anim & Poultry Sci,Key Lab A, Changsha 410125, Hunan, Peoples R China.;[Liu, C. X.; Hu, Q.] Anyou Biotechnol Grp Co Ltd, Taicang City 215437, Jiangsu, Peoples R China.;[Hou, Y. Q.] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Nyachoti, C. M.] Univ Manitoba, Dept Anim Sci, Winnipeg, MB R3T 2N2, Canada.;[Xiao, D. F.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, C. X.] A;Anyou Biotechnol Grp Co Ltd, Taicang City 215437, Jiangsu, Peoples R China.
关键词:
barrier functions;chito-oligosaccharide;growth performance;immune response;intestinal morphology;piglets
摘要:
This study was conducted to determine the effect of dietary supplementation of a low dose of chito-oligosaccharide (COS) on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets. A total of 120 weaned pigs (21 d of age; 7.86 ± 0.22 kg average BW) were randomly assigned (6 pens/diet; 10 pigs/pen) to 2 dietary treatments consisting of a basal diet (negative control) or the basal diet supplemented with COS (30 mg/kg) for a 14-d period. Six randomly selected piglets from each treatment were killed for blood and tissue sampling. No significant differences were observed in ADG, ADFI, and G:F between treatment and the control group. Piglets fed the COS-supplemented diet had greater (P < 0.05) stomach pH than those fed the control diet on d 14 postweaning. Dietary supplementation with COS reduced villus height (P < 0.05) and villus height:crypt depth (P < 0.05) in the ileum. Dietary COS supplementation tended to reduce villus height in the duodenum (P = 0.065) and jejunum (P = 0.058). There was no effect on crypt depth in the intestinal segments of treatment group. Piglets fed the COS-supplemented diet increased (P < 0.05) the number of intraepithelial lymphocytes in duodenum or jejunum and goblet cells of ileum. However, COS decreased (P < 0.05) the number of intraepithelial lymphocytes in ileum of weaned piglets. The concentrations of IL-10 (duodenum, jejunum, and ileum) and secretory immunoglobulin (SIgA; duodenum and ileum) were higher in piglets fed the COSsupplemented diet compared with control (P < 0.05). Dietary COS supplementation reduced (P < 0.05) the concentration of total antioxidant capacity and superoxide dismutase of the jejunum or ileum. The mRNA expression of occludin in the ileum and ZO-1 in jejunum and ileum had a significant change in piglets fed the COS-supplemented diet compared with the control group (P < 0.05). In conclusion, these results indicated that dietary COS supplementation at 30 mg/kg had no effects on promoting growth performance and tended to reduce villus height in the duodenum or jejunum of weaned piglets. The results further showed that supplemental COS at this level may cause an immune and oxidative stress response in small intestine and have compromised the intestinal barrier integrity in weaned piglets. The research will provide guidance on the low dosage of COS supplementation on weaning pigs. © 2015 American Society of Animal Science. All rights reserved.
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英文
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The effects of enzyme supplementation on performance and digestive parameters of broilers fed corn-soybean diets
作者:
Zhu, H. L.;Hu, L. L.;Hou, Y. Q.;Zhang, J.;Ding, B. Y.*
期刊:
Poultry Science ,2014年93(7):1704-1712 ISSN:0032-5791
通讯作者:
Ding, B. Y.
作者机构:
[Zhu, H. L.; Hou, Y. Q.; Ding, B. Y.; Hu, L. L.; Zhang, J.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Ding, B. Y.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
broiler;enzyme supplementation;dietary metabolizable energy level;growth performance;digestive parameter
摘要:
The study was performed to evaluate the effects of enzyme supplementation on performance and digestive parameters of broilers fed corn-soybean diets from 1 to 21 d of age. A total of 480 one-day-old Cobb broilers were allocated to 1 of 4 treatments, with 6 replicate pens per treatment and 20 birds per pen. The experiment consisted of a 2 × 2 factorial arrangement of treatments with 2 dietary ME levels (high ME, energy 1, 12.13 MJ/kg or low ME, energy 2, 11.92 MJ/kg) and 2 levels of supplemental enzyme (including xylanase, 1,800 IU/g, β-glucanase, 500 IU/g, and α-amylase, 800 U/g; 0 or 0.1% of diet). Enzyme supplementation had no effect on average daily weight gain, feed intake, and feed:gain. However, enzyme supplementation decreased the relative weight of the pancreas (d 7 and 21) in broilers fed the high ME diet. Low dietary ME level increased pancreatic lipase (d 7, P = 0.015), trypsin (d 14, P = 0.01; d 21, P = 0.014), amylase (d 21, P = 0.027), and pepsin (d 7, P = 0.001; d 21, P = 0.042) activity, but reduced pancreatic lipase (d 14, P = 0.03; d 21, P = 0.004) and amylase (d 14, P = 0.027) activity. Enzyme supplementation resulted in an increase in pancreatic amylase (d 7, P = 0.023), trypsin (d 7, P = 0.02; d 21, P = 0.004), lipase (d 21, P = 0.001), pepsin (d 7, P = 0.001; d 14, P = 0.004; d 21, P = 0.001), and maltase (d 14, P = 0.011, in ileum) activity. Moreover, broilers fed low dietary ME and enzyme supplementation diets had an increase in pancreatic lipase (d 21, P = 0.001) and pepsin (d 7, P = 0.001) activity. Low ME diets reduced jejunum villus height and jejunum and ileum crypt depth (d 7, 21). However, enzyme supplementation, especially enzyme supplementation in low ME diets, increased jejunum and ileum villus height and villus surface area. This suggested enzyme supplemented with low ME diet might be more effective to improve the activity of digestive enzymes and the absorptive capacity of the small intestine.
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英文
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