TLR4信号通路关键基因在断奶仔猪不同组织中的mRNA表达
作者：
陈逢;刘玉兰;李权;朱惠玲;侯永清;...
期刊：
中国畜牧杂志 ,2013年49(17):53-57 ISSN：0258-7033
作者机构：
[陈逢; 刘玉兰; 李权; 朱惠玲; 侯永清; 易丹; 陈洪波] 武汉工业学院动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会
会议时间：
2012-10-19
会议地点：
长沙
会议论文集名称：
中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会论文集
关键词：
TLR4信号通路;实时荧光定量PCR;断奶仔猪
摘要：
本试验旨在研究Toll样受体4(TLR4)信号通路关键基因在断奶仔猪不同组织的分布情况。选择12头杜×长×大断奶仔猪,屠宰,取脾脏、胸腺、肠道淋巴结、下丘脑、垂体、肾上腺、肝脏、腓肠肌、皮下脂肪、空肠和回肠组织。应用实时荧光定量PCR技术测定TLR4信号通路关键基因,包括TLR4、髓样分化因子(MyD88)、IL-1受体相关激酶1(IRAK1)、肿瘤坏死因子受体相关因子6(TRAF6)、核因子-κB(NF-κB)和肿瘤坏死因子(TNF)-α在各组织的mRNA表达水平。结果表明:TLR4信号通路关键基因在所检测的11个组织中均有表达,并且组织分布规律基本一致。总体看来,主要在免疫组织(脾脏、胸腺、淋巴结)表达量较高,在皮下脂肪和肠道(空肠、回肠)表达量居中,在其他组织中表达量较低。TLR4信号通路关键基因在不同组织中表达差异较大,可能与仔猪各组织对病原的识别和抵抗能力有关。
语种：
中文
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仔猪溃疡性结肠炎模型的建立
作者：
陈星;王清静;王蕾;朱惠玲;丁斌鹰;...
期刊：
饲料工业 ,2013年34(19):24-27 ISSN：1001-991X
作者机构：
动物营养与饲料科学湖北省重点实验室武汉轻工大学,湖北武汉,430023;[陈星; 丁斌鹰; 侯永清; 王清静; 王蕾; 朱惠玲] 武汉轻工大学
关键词：
仔猪;结肠炎;乙酸
摘要：
试验旨在建立乙酸诱导仔猪溃疡性结肠炎模型。选取18头健康仔猪（杜洛克×长白×大白）,随机分成3组:对照组（3头）、三硝基苯磺酸（TNBS）组（6头）和乙酸组（9头）,分别注射30 mg/kg BW戊巴比妥钠,待完全麻醉后,对照组仔猪直肠灌注10 ml无菌生理盐水,TNBS组分别灌注25 mg/kg BW（3头）和50 mg/kg BW（3头）TNBS的50%乙醇溶液,乙酸组分别灌注10 ml 5%（3头）和10%的乙酸（6头）。随时观察仔猪状态与粪便情况,灌注3 d或7 d后屠宰,并取结肠组织固定,制作病理切片观察损伤变化,分别进行黏膜损伤评分,以判定造模是否成功。结果表明,采用直肠灌注不同浓度TNBS（25 mg/kg BW和50 mg/kg BW）、5%乙酸,均只出现短暂的稀便,病理切片观察只有轻微炎性症状,与对照组没有显著差异;灌注10%乙酸的6头仔猪,根据粪便性状及结肠肉眼观察均显示明显的结肠炎症状,同时通过组织学观察发现,3 d后出现溃疡灶,并有明显的炎性细胞浸润等炎症反应,而7 d后可见溃疡灶已脱落,炎性细胞浸润明显减少,大量成纤维细胞增生。由此可见,直肠灌注10%乙酸能成功诱导仔猪结肠炎。
语种：
中文
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Dietary l-proline supplementation confers immunostimulatory effects on inactivated Pasteurella multocida vaccine immunized mice
作者：
Ren, Wenkai;Zou, Lingxiu;Ruan, Zheng;Li, Nengzhang;Wang, Yan;...
期刊：
Amino Acids ,2013年45(3):555-561 ISSN：0939-4451
通讯作者：
Yin, Yulong
作者机构：
[Liu, Gang; Ren, Wenkai; Li, Tiejun; Wu, Guoyao; Yin, Yulong] Chinese Acad Sci, Minist Agr,Sci Observin & Expt Stn Anim Nutr & Fe, Key Lab Agroecol & Proc Subtrop Reg,Hunan Engn &, Inst Subtrop Agr,Res Ctr Hlth Breeding Livestock, Changsha 410125, Hunan, Peoples R China.;[Li, Nengzhang; Ren, Wenkai; Peng, Yuanyi; Zou, Lingxiu; Wang, Yan] Southwest Univ, Chongqing Key Lab Forage & Herbivores, Coll Anim Sci & Technol, Chongqing 400716, Peoples R China.;[Yin, Yulong; Ruan, Zheng] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构：
[Yin, Yulong] C;Chinese Acad Sci, Minist Agr,Sci Observin & Expt Stn Anim Nutr & Fe, Key Lab Agroecol & Proc Subtrop Reg,Hunan Engn &, Inst Subtrop Agr,Res Ctr Hlth Breeding Livestock, Changsha 410125, Hunan, Peoples R China.
关键词：
Proline;Pasteurella multocida;Immunostimulatory effect;Adjuvant
摘要：
This study was conducted to determine the immunostimulatory effect of l-proline on inactivated vaccine immunized mice. Ninety-five female KM mice were randomly divided into five groups: (1) mice received dietary supplementation with 0.4 % l-proline and immunized with inactivated vaccine (V-P group); (2) mice received dietary supplementation with 0.3 % l-alanine (isonitrogenous control) and immunized with inactivated vaccine (V-A group, negative control); (3) mice were immunized with inactivated vaccine with oil adjuvant (V-O group, positive control); (4) mice were immunized with inactivated vaccine with aluminum hydroxide adjuvant (V-H group, positive control); (5) mice immunized with phosphate-buffered saline (control group). All mice were dead in the control group between 36 and 48 h post infection. Mice in the V-P group showed 100 % protection after challenge with P. multocida serotype A (CQ2) at dose of 4.4 × 105 CFU (2LD50). Meanwhile, serum antibody titers in the V-P group were higher than those in the V-A group before infection and those in the V-A and V-O groups at 36 h post infection. Moreover, serum IL-1β levels in the V-P group were lower than those in V-O group. Furthermore, serum GSH-PX levels in the V-P group were higher than those in the V-A and V-O groups. Collectively, dietary proline supplementation confers beneficial immunostimulatory effects in inactivated P. multocida vaccine immunized mice. © 2013 Springer-Verlag Wien.
语种：
英文
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Effects of enzyme complex supplementation to a paddy-based diet on performance and nutrient digestibility of meat-type ducks
作者：
Kang, P.;Hou, Y. Q.;Toms, Derek;Yan, N. D.;Ding, B. Y.* ;...
期刊：
ANIMAL BIOSCIENCE ,2013年26(2):253-259 ISSN：2765-0189
通讯作者：
Ding, B. Y.
作者机构：
[Hou, Y. Q.; Ding, B. Y.; Yan, N. D.; Kang, P.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.;[Toms, Derek] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON, Canada.
通讯机构：
[Ding, B. Y.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
Xylanase;beta-Glucanase;Cellulase;Paddy;Ducks
摘要：
Paddy rice is rarely used as a feed because of its high fiber content. In this study, two experiments were conducted to study the effects of supplementing an enzyme complex consisting of xylanase, beta-glucanase and cellulase, to paddy-based diets on the performance and nutrient digestibility in meat-type ducks. In the both experiments, meat-type ducks (Cherry Valley) were randomly assigned to four treatments. Treatment 1 was a basal diet of corn-soybean; treatment 2 was a basal diet of corn-paddy-soybean; treatment 3, had enzyme complex added to the corn-paddy-soybean basal diet at levels of 0.5 g/kg diet; and treatment 4, had enzyme complex added to the corn-paddy-soybean diet at levels of 1.0 g/kg diet. The results showed that the enzyme complex increased the ADG, and decreased the ADFI and F/G significantly (p<0.05) in the ducks, and the ADFI for the ducks fed the corn-paddy-soybean diet showed no difference compared to the ducks fed corn-soybean diets at all stages of the experiment (p<0.05). When corn was partially replaced by paddy, the digestibility of CP and NDF was decreased and increased, respectively (p<0.05), and the level of enzyme complex had a significant effect on both CP and NDF digestibility (p<0.05). As for the AME, addition of enzyme complex increased it significantly (p<0.05), but both diet types and levels of enzyme complex had no effect (p>0.05). The outcome of this research indicates that the application of enzyme complex made up of xylanase, beta-glucanase, and cellulase, in the corn-paddy-soybean diet, can improve performance and nutrition digestibility in meat-type ducks. Copyright © 2013 by Asian-Australasian Journal of Animal Sciences.
语种：
英文
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Fish Oil Alleviates Activation of the Hypothalamic-Pituitary-Adrenal Axis Associated with Inhibition of TLR4 and NOD Signaling Pathways in Weaned Piglets after a Lipopolysaccharide Challenge
作者：
Liu, Yulan* ;Chen, Feng;Li, Quan;Odle, Jack;Lin, Xi;...
期刊：
The Journal of Nutrition ,2013年143(11):1799-1807 ISSN：0022-3166
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Li, Quan; Hou, Yongqing; Shi, Haifeng; Chen, Feng; Pi, Dingan; Hong, Yu; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
摘要：
Long-chain n-3 (ω-3) polyunsaturated fatty acids exert beneficial effects in neuroendocrine dysfunctions in animal models and clinical trials. However, the mechanism(s) underlying the beneficial effects remains to be elucidated. We hypothesized that dietary treatment with fish oil (FO) could mitigate LPS-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis through inhibition of Toll-like receptor 4 and nucleotide-binding oligomerization domain protein signaling pathways. Twentyfour weaned pigs were used in a 2 × 2 factorial design, and the main factors consisted of diet (5% corn oil vs. 5% FO) and immunological challenge (saline vs. LPS). After 21 d of dietary treatment with 5% corn oil or FO diets, pigs were treated with saline or LPS. Blood samples were collected at 0 (preinjection), 2, and 4 h postinjection, and then pigs were humanely killed by intravenous injection of 40 mg/kg body weight sodium pentobarbital for tissue sample collection. FO led to enrichment of eicosapentaenoic acid and docosahexaenoic acid and total n-3 polyunsaturated fatty acids in hypothalamus, pituitary gland, adrenal gland, spleen, and thymus. FO decreased plasma adrenocorticotrophin and cortisol concentrations as well as mRNA expressions of hypothalamic corticotropin releasing hormone and pituitary proopiomelanocortin. FO also reduced mRNA expression of tumor necrosis factor-α in hypothalamus, adrenal gland, spleen, and thymus, and of cyclooxygenase 2 in hypothalamus. Moreover, FO downregulated the mRNA expressions of Toll-like receptor 4 (TLR4) and its downstream molecules, including cluster differentiation factor 14,myeloid differentiation factor 2, myeloid differentiation factor 88, interleukin- 1 receptor-associated kinase 1, tumor necrosis factor-α receptor-associated factor 6, and nuclear factor kappa-light-chainenhancer of activated B cells p65, and also decreased the mRNA expressions of nucleotide-binding oligomerization domain 1, nucleotide-binding oligomerization domain 2, and their adaptor molecule receptor-interacting serine/threonine-protein kinase 2. These results suggested that FOattenuates the activation of the HPA axis induced by LPS challenge. The beneficial effects of FO on the HPA axis may be associated with decreasing the production of brain or peripheral proinflammatory cytokines through inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways. © 2013 American Society for Nutrition.
语种：
英文
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N-acetylcysteine reduces inflammation in the small intestine by regulating redox, EGF and TLR4 signaling
作者：
Hou, Yongqing* ;Wang, Lei;Yi, Dan;Ding, Binying;Yang, Zhenguo;...
期刊：
Amino Acids ,2013年45(3):513-522 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Hou, Yongqing; Yang, Zhenguo; Chen, Xing; Wang, Lei; Qiu, Yinsheng; Ding, Binying; Li, Jiao; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
N-acetylcysteine;Intestinal mucosa;Lipopolysaccharide;Epidermal growth factor;Toll like receptor;Aquaporin
摘要：
This study determined whether N-acetylcysteine (NAC) could affect intestinal redox status, proinflammatory cytokines, epidermal growth factor (EGF), EGF receptor (EGFR), Toll-like receptor-4 (TLR4), and aquaporin-8 in a lipopolysaccharide (LPS)-challenged piglet model. Eighteen piglets (35-day-old) were randomly allocated into one of the three treatments (control, LPS and NAC). The control and LPS groups were fed a basal diet, and the NAC group received the basal diet +500 mg/kg NAC. On days 10, 13, and 20 of the trial, the LPS- and NAC-treated piglets received intraperitoneal administration of LPS (100 μg/kg BW), whereas the control group received the same volume of saline. On days 10 and 20, venous blood samples were obtained at 3 h post LPS or saline injection. On day 21 of the trial, piglets were killed to obtain the intestinal mucosa for analysis. Compared with the control group, LPS challenge reduced (P < 0.05) the activities of superoxide dismutase, catalase, and glutathione peroxidase in jejunal mucosae, while increasing (P < 0.05) the concentrations of malondialdehyde, H2O2, O2 ·- and the ratio of oxidized to reduced glutathione in jejunal mucosae, and concentrations of TNF-α, cortisol, interleukin-6, and prostaglandin E2 in both plasma and intestinal mucosae. These adverse effects of LPS were attenuated (P < 0.05) by NAC supplementation. Moreover, NAC prevented LPS-induced increases in abundances of intestinal HSP70 and NF-κB p65 proteins and TLR4 mRNA. NAC supplementation enhanced plasma EGF concentration and intestinal EGFR mRNA levels. Collectively, these results indicate that dietary NAC supplementation alleviates LPS-induced intestinal inflammation via regulating redox, EGF, and TLR4 signaling. © 2012 Springer-Verlag.
语种：
英文
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鱼油对脂多糖刺激仔猪肠黏膜能量代谢的影响
作者：
王秀英;刘玉兰;李藏兰;陈逢;朱惠玲;...
期刊：
中国畜牧杂志 ,2013年49(23):22-25 ISSN：0258-7033
作者机构：
1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室;2. 中国农业大学动物科技学院;3. 福建高龙实业有限公司;[李藏兰] 中国农业大学;[丁斌鹰; 侯永清; 李爽; 王秀英; 朱惠玲; 陈逢; 刘玉兰; 石海峰] 武汉轻工大学
关键词：
鱼油;脂多糖;肠黏膜;能量代谢;仔猪
摘要：
试验研究鱼油对脂多糖(LPS)刺激仔猪肠黏膜能量代谢的影响。选用32头(28±3)d(8.91±0.74)kg的杜洛克×长白×大白仔猪,采用2×2因子设计,主因子包括日粮处理(5%鱼油或5%玉米油)和免疫应激(注射LPS或生理盐水),试验期19 d。试验第19天,每日粮组一半的猪注射100μg/kg BW的LPS,另一半注射生理盐水作对照。注射LPS 4 h后屠宰仔猪,取小肠样品待测。结果表明:鱼油对肠黏膜能量代谢的影响与LPS刺激不存在显著的互作关系;LPS刺激导致结肠三磷酸腺苷(ATP)水平显著下降(P<0.05);鱼油显著提高了结肠三磷酸腺苷(ATP)、一磷酸腺苷(AMP)、腺苷酸池(TAN)水平(P<0.05),对结肠AMP/ATP比值有提高的趋势(P<0.10),鱼油也显著降低了结肠能荷(EC)水平(P<0.05);鱼油和LPS刺激对空肠和回肠能量代谢指标均无显著影响。以上研究结果显示,鱼油可以缓解LPS应激引起的仔猪结肠黏膜能量代谢障碍,从而保护肠黏膜屏障。
语种：
中文
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L-Glutamine supplementation increases Pasteurella multocida burden and expression of major virulence factors in mice
作者：
Wenkai Ren;Gang Liu;Yongqing Hou;Martin Nyachoti;Tiejun Li;...
作者机构：
[Wenkai Ren; Shuping Liu; Fengmei Zhang; Nengzhang Li; Yuanyi Peng] Chongqing Key Laboratory of Forage & Herbivorce,College of Animal Science and Technology,Southwest University,Chongqing 400716,China;[Wenkai Ren; Gang Liu; Tiejun Li; Guoyao Wu; Shuai Chen; Jie Yin; Yulong Yin; Li Wu] Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central,Ministry of Agriculture,Hunan Provincial Engineering Research Center of Healthy Livestock,Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;[Yongqing Hou; Yulong Yin] Hubei key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China;[Martin Nyachoti] Department of Animal Science,University of Manitoba,Winnipeg,R3T 2N2,MB,Canada;[Guoyao Wu] Department of Animal Science,Texas A&M University,College Station,TX77843,USA
会议名称：
第六届动物营养、保健与饲料添加剂国际学术研讨会
会议时间：
2013-7-22
会议地点：
青岛
会议主办单位：
国家生猪产业技术创新战略联盟
会议论文集名称：
第六届动物营养、保健与饲料添加剂国际学术研讨会论文集
关键词：
Glutamine;Pasteurella multocida;virulence factor;TLR signaling
摘要：
This study was conducted to determine effects of graded doses of glutamine supplementation on the replication and distribution of Pasteurella multocida,and expression of major virulence factors in a mouse model.Mice were randomly assigned into the basal diet supplemented with 0%,0.5%,1.0% or 2.0% glutamine.Pasteurella multocida burden were detected in heart,liver,spleen,lung and kidney after 12 hrs of Pasteurella multocida infection.Meanwhile,the expression of major virulence factors,toll-like receptors (TLRs),proinflammatory cytokines (IL-1β,IL-6 and TNF-a),and anti-oxidative factors (GPX1 and CuZnSOD) was analyzed in the lung and spleen.Dietary 0.5% glutamine supplementation have no much significant effect on these data,compared to those with basa diet.However,our study showed that a high dose of glutamine supplementation increased the P.multocida burden (P<0.001) and expression of the genes for major virulence factors expression of (P<0.05),when compared to those with a low-dose group.In the lung,a high dose of glutamine supplementation inhibited proinflammatory responses (P<0.05) and TLRs signaling (P<0.05).In the spleen,the effect of glutamine supplementation on different components in TLR signaling depends on glutamine concentration,as a high dose of supplemental glutamine activated the proinflammatory response.In conclusion,glutamine supplementation increased P.multocida burden and expression of major virulence factors,while affecting the functions of the lung and spleen.
语种：
英文
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The combination effects of acetaminophen and N-acetylcysteine on cytokines production and NF-κB activation of lipopolysaccharide-challenged piglet mononuclear phagocytes in vitro and in vivo
作者：
Qiu, Yinsheng* ;Zhang, Jiawei;Liu, Yu;Ma, Hongwei;Cao, Fangyuan;...
期刊：
Veterinary Immunology and Immunopathology ,2013年152(3-4):381-388 ISSN：0165-2427
通讯作者：
Qiu, Yinsheng
作者机构：
[Liu, Yu; Xu, Jun; Hou, Yongqing; Zhang, Jiawei; Qiu, Yinsheng; Cao, Fangyuan; Ma, Hongwei] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.;[Xu, LingYun] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Qiu, Yinsheng] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
关键词：
Acetaminophen;N-acetylcysteine;Cytokines;NF-kappa B;Mononuclear phagocytes;Piglets
摘要：
Lipopolysaccharide (LPS) is a known activator of mononuclear phagocytes. LPS activates the pro-inflammatory gene expression and induces the release of mediators/cytokines by TLR4-NF-kappa B signaling pathway. The purpose of this study was to investigate the effects of acetaminophen (AAP) and N-acetylcysteine (NAC), individually as well as in combination on LPS-induced cytokines production and NF-kappa B activation in piglets. AAP (0.125-1.0 mM) and NAC (0.0625-1.0 mM) down-regulate the expression of cytokines and inhibit NF-kappa B p65 protein transfer from the cytoplasm to the nucleus in vitro. NAC enhances the inhibition action of AAP on cytokines expression in vitro. IL-6 in piglet plasma of the AAP group (mixed feed concentration of 600 mg/kg) was significantly reduced (P<0.05) at3 h after LPS-challenge as compared with the LPS control group. IL-10 also significantly reduced (P<0.05) at 24 h after LPS injection. The levels of inflammatory cytokines (TNF-alpha, IL-1p, IL-6, and IL10) in piglet plasma of the NAC group (mixed feeding concentration of 1200 mg/kg) were significantly lower at 3 h after LPS stimulation (P<0.05). IL-10 was significantly decreased in the NAC group at 24 h after LPS stimulation (P<0.05). AAP or NAC treated alone could reduce the NF-kappa B p65 concentration ratio. The levels of cytokines (TNF-alpha, IL-1 p, IL-6, and IL-10) in the group with piglet plasma of AAP (mixed feed concentration of 600 mg/kg) plus NAC (mixed feeding concentration of 1200 mg/kg) group were significantly lower (P<0.05) at 3 h after LPS activation. The level of IL-10 in the group with AAP plus NAC was significantly lower (P<0.05) at 24h after LPS stimulation, while the rest of the inflammatory cytokines were returned to the original levels. The NF-kappa B p65 concentration ratio had significantly reduced (P<0.05) when AAP and NAC were used in combination. In summary, NAC could enhance the anti-inflammatory effects of AAP both in vitro and in vivo. (C) 2013 Elsevier B.V. All rights reserved.
语种：
英文
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Effects of dietary l-lysine intake on the intestinal mucosa and expression of CAT genes in weaned piglets
作者：
He, Liuqin;Yang, Huansheng;Hou, Yongqing;Li, Tiejun* ;Fang, Jun;...
期刊：
Amino Acids ,2013年45(2):383-391 ISSN：0939-4451
通讯作者：
Li, Tiejun
作者机构：
[Wu, Li; Li, Tiejun; Wu, Guoyao; Yin, Yulong; He, Liuqin; Yang, Huansheng] Chinese Acad Sci, Key Lab Agroecol Subtrop Reg,Inst Subtrop Agr Res, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Minist Agr,Hunan Engn & Res Ctr Anim & Poultry Sc, Changsha 410125, Hunan, Peoples R China.;[Hou, Yongqing; Yin, Yulong] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhou, Xihong] Zhejiang Univ, Key Lab Mol Anim Nutr, Inst Feed Sci, Hangzhou 310012, Zhengjiang, Peoples R China.;[Nyachoti, Martin] Univ Manitoba, Dept Anim Sci, Winnipeg, MB R3T 2N2, Canada.
通讯机构：
[Li, Tiejun] C;Chinese Acad Sci, Key Lab Agroecol Subtrop Reg,Inst Subtrop Agr Res, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Minist Agr,Hunan Engn & Res Ctr Anim & Poultry Sc, Changsha 410125, Hunan, Peoples R China.
关键词：
Pigs;Digestibility;Cationic amino acids;Intestinal mucosa;Transporters
摘要：
The objective of this study was to evaluate effects of dietary l-lysine on the intestinal mucosa and expression of cationic amino acid transporters (CAT) in weaned piglets. Twenty-eight piglets weaned at 21 days of age (Duroc × Landrace × Yorkshire; 6.51 ± 0.65 kg body weight) were assigned randomly into one of the four groups: Zein + LYS (zein-based diet + 1.35 % supplemental lysine), Zein - LYS (zein-based diet), NF (nitrogen-free diet), and CON (basal diet). The experiment lasted for 3 weeks, during which food intake and body weight were recorded. At the end of the trial, blood was collected from the jugular vein of all pigs, followed by their euthanasia. Dietary supplementation with lysine enhanced villus height and crypt depth in the jejunum (P < 0.05). Jejunal mRNA levels for the b0,+-AT, y +LAT1 and CAT1 genes were greater (P < 0.05) in the Zein + LYS group than in the control, and the opposite was observed for CAT1. Dietary content of lysine differentially affected intestinal CAT expression to modulate absorption of lysine and other basic amino acids. Thus, transport of these nutrients is a key regulatory step in utilization of dietary protein by growing pigs and lysine in the diet influences the expression of amino acid transporters in the small intestine. © 2013 Springer-Verlag Wien.
语种：
英文
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Fish Oil Increases Muscle Protein Mass and Modulates Akt/FOXO, TLR4, and NOD Signaling in Weanling Piglets After Lipopolysaccharide Challenge
作者：
Liu, Yulan* ;Chen, Feng;Odle, Jack;Lin, Xi;Zhu, Huiling;...
期刊：
The Journal of Nutrition ,2013年143(8):1331-1339 ISSN：0022-3166
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Shi, Haifeng; Hou, Yongqing; Chen, Feng; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.;[Yin, Jingdong] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100094, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
摘要：
Proinflammatory cytokines play a key role in the pathophysiology of muscle atrophy. In addition, n3 polyunsaturated fatty acids (PUFAs) exert an inhibitory effect on proinflammatory cytokines affecting many inflammatory diseases. We hypothesized that dietary supplementation of fish oil could attenuate lipopolysaccharide (LPS)-induced muscle atrophy.Weanling pigswere used in a 2 × 2 factorial design and themain factors included diet (5% corn oil or 5%fish oil) and immunological challenge (LPS or saline). After 21 d of treatment with either fish oil or corn oil, pigs received an i.p. injection of either saline or LPS. At 4 h postinjection, blood and muscle samples were obtained. Fish oil led to enrichment of eicosapentaenoic acid, docosahexaenoic acid, and total n3 PUFAs in muscles. Fish oil increased muscle protein mass, indicated by a higher protein:DNA ratio in gastrocnemius and longissimus dorsi (LD) muscles. In addition, fish oil increased Akt1 mRNA abundance and decreased Forkhead Box O (FOXO) 1 and FOXO4 mRNA abundance. Fish oil also increased phosphorylation of Akt and FOXO1 in gastrocnemius and LD muscles. Fish oil decreased the mRNA abundance of muscle atrophy F-box (MAFbx) and muscle RING finger 1 in gastrocnemius and LD muscles. Moreover, fish oil reduced the plasma tumor necrosis factor (TNF) a, muscle TNFa, and prostaglandin E2 concentrations, and muscle TNFa and cyclooxygenase 2 (COX2) mRNA abundance. Finally, fish oil downregulated the mRNA abundance ofmuscle toll-like receptor (TLR4) and its downstream signalingmolecules [myeloid differentiation factor 88 (MyD88), TNFa receptor-associated factor 6 (TRAF6), and NF-kB p65], and nucleotide-binding oligomerization domain protein (NOD1), NOD2, and their adaptor molecule [receptor-interacting serine/threonine-protein kinase 2 (RIPK2)]. These results indicate fish oil may suppress muscle proinflammatory cytokine production via regulation of TLR and NOD signaling pathways and therefore improve muscle protein mass, possibly through maintenance of Akt/FOXO signaling. © 2013 American Society for Nutrition.
语种：
英文
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Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model
作者：
Wang, Qingjing;Hou, Yongqing* ;Yi, Dan;Wang, Lei;Ding, Binying;...
期刊：
BMC Gastroenterology ,2013年13(1):1-11 ISSN：1471-230X
通讯作者：
Hou, Yongqing
作者机构：
[Long, Minhui; Liu, Yulan; Hou, Yongqing; Chen, Xing; Wang, Lei; Wang, Qingjing; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
N-acetylcysteine;Acetic acid;Colon injury;Claudin-1;Epidermal growth factor;Amphiregulin
摘要：
Background: Ulcerative colitis is a chronic inflammatory disease and involves multiple etiological factors. Acetic acid (AA)-induced colitis is a reproducible and simple model, sharing many characteristics with human colitis. N-acetylcysteine (NAC) has been widely used as an antioxidant in vivo and in vitro. NAC can affect several signaling pathways involving in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and inflammatory response. Therefore, NAC may not only protect against the direct injurious effects of oxidants, but also beneficially alter inflammatory events in colitis. This study was conducted to investigate whether NAC could alleviate the AA-induced colitis in a porcine model.Methods: Weaned piglets were used to investigate the effects of NAC on AA-induced colitis. Severity of colitis was evaluated by colon histomorphology measurements, histopathology scores, tissue myeloperoxidase activity, as well as concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon. The protective role of NAC was assessed by measurements of antioxidant status, growth modulator, cell apoptosis, and tight junction proteins. Abundances of caspase-3 and claudin-1 proteins in colonic mucosae were determined by the Western blot method. Epidermal growth factor receptor, amphiregulin, tumor necrosis factor-alpha (TNF-α), and toll-like receptor 4 (TLR4) mRNA levels in colonic mucosae were quantified using the real-time fluorescent quantitative PCR.Results: Compared with the control group, AA treatment increased (P < 0.05) the histopathology scores, intraepithelial lymphocyte (IEL) numbers and density in the colon, myeloperoxidase activity, the concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon, while reducing (P < 0.05) goblet cell numbers and the protein/DNA ratio in the colonic mucosa. These adverse effects of AA were partially ameliorated (P < 0.05) by dietary supplementation with NAC. In addition, NAC prevented the AA-induced increase in caspase-3 protein, while stimulating claudin-1 protein expression in the colonic mucosa. Moreover, NAC enhanced mRNA levels for epidermal growth factor and amphiregulin in the colonic mucosa.Conclusion: Dietary supplementation with NAC can alleviate AA-induced colitis in a porcine model through regulating anti-oxidative responses, cell apoptosis, and EGF gene expression. © 2013 Wang et al.; licensee BioMed Central Ltd.
语种：
英文
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Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways
作者：
Chen, Feng;Liu, Yulan* ;Zhu, Huiling;Hong, Yu;Wu, Zhifeng;...
期刊：
INNATE IMMUNITY ,2013年19(5):504-515 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Wu, Zhifeng; Li, Quan; Hou, Yongqing; Chen, Feng; Hong, Yu; Ding, Binying; Yi, Dan; Zhu, Huiling; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Fish oil;lipopolysaccharide;weaned pigs;liver injury;pro-inflammatory cytokines;Toll-like receptor 4;nucleotide-binding oligomerization domain protein
摘要：
This study evaluated whether fish oil exerted a hepatoprotective effect in a LPS-induced liver injury model via regulation of TLR4 and nucleotide-binding oligomerization domain protein (NOD) signaling pathways. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline). Fish oil resulted in enrichment of eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acids in liver. Less severe liver injury was observed in pigs fed fish oil, as evidenced by improved serum biochemical parameters and less severe histological liver damage. In addition, higher expression of liver tight junction proteins, and lower hepatocyte proliferation and higher hepatocyte apoptosis were observed in pigs fed fish oil. The improved liver integrity in pigs fed fish oil was concurrent with reduced hepatic mRNA expression of TLR4, myeloid differentiation factor 88, IL-1 receptor-associated kinase 1 and TNF-α receptor-associated factor 6, and NOD1, NOD2 and receptor-interacting serine/threonine-protein kinase 2, as well as reduced hepatic protein expression of NF-κB p65, leading to reduced hepatic pro-inflammatory mediators. These results indicate that fish oil improves liver integrity partially via inhibition of TLR4 and NOD signaling pathways under an inflammatory condition. © 2013 The Author(s).
语种：
英文
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Effect of l-arginine on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge
作者：
Zhu, H. L.;Liu, Y. L.* ;Xie, X. L.;Huang, J. J.;Hou, Y. Q.
期刊：
INNATE IMMUNITY ,2013年19(3):242-252 ISSN：1753-4259
通讯作者：
Liu, Y. L.
作者机构：
[Zhu, H. L.; Liu, Y. L.; Hou, Y. Q.; Xie, X. L.; Huang, J. J.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Y. L.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Lipopolysaccharide;arginine;intestinal mucosal immune barrier function;weaned pigs
摘要：
The effects of l-arginine (Arg) supplementation on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge were evaluated. Twenty-four weaned pigs were allotted to four treatments including: (i) non-challenged control; (ii) LPS-challenged control; (iii) LPS + 0.5% Arg; and (iv) LPS + 1.0% Arg. On d 16, pigs in the LPS, LPS + 0.5% Arg and LPS + 1.0% Arg groups were challenged by injection with 100 μg/kg of body mass LPS, whereas the control group were given sterile saline. At 48 h post-challenge, all pigs were sacrificed for evaluation of small intestinal morphology and mucosal immune barrier function. In the jejunum and ileum, LPS caused villous atrophy and intestinal morphology disruption, whereas 0.5% or 1.0% Arg supplementation mitigated villus atrophy and intestinal morphology impairment caused by LPS challenge. Arg (0.5%) supplementation increased the numbers of IgA-secreting cells, CD8+ and CD4+ T cells in the ileum (P < 0.05). Arg supplementation prevented the elevation of mast cell numbers induced by LPS challenge (P < 0.05). Dietary supplementation of Arg caused a decreased lymphocyte apoptosis of Peyer's patches in pigs challenged by LPS (P < 0.05). These results indicated that Arg supplementation protects and enhances intestinal mucosal immune barrier function and maintains intestinal integrity in weaned pigs after E. coli LPS challenge. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
语种：
英文
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Effects of monosodium glutamate supplementation on blood lipid, amino acid and hormone and colonic organic acid concentrations in sow-reared piglets
作者：
Jun Zhang;Bie Tan;Guoyao Wu;Gang Chen;Yulong Yin;...
作者机构：
[Jun Zhang; Bie Tan; Gang Chen; Yulong Yin; Fengna Li; Ruiling Huang; Tiejun Li] Chinese Acad Sci,Inst Subtrop Agr,Res Ctr Healthy Breeding Livestock & Poultry,Hunan Engn & Res Ctr Anim & Poultry Sci,Key Lab Agroecol Proc Subtrop Reg,Scientific Oberving and Experimetal Station of Animal Nutrition and Feed Science in South-Central,Ministry of Agriculture,Changsha City,Furong Road ＃644,Hunan,Peoples R China 410125;[Guoyao Wu] Department of Animal Science,Texas A&M University,College Station,TX 77843,USA;[Yulong Yin; Yongqing Hou] Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China;[Fran(c)ois Blachier] UMR 914 INRA/AgroParisTech,Nutrition Physiology and Alimentary Behavior,16 rue Claude Bernard,75005 Paris,France;[Martin Nyachoti] Department of Animal Science,University of Manitoba,Winnipeg,Canada R3T 2N2,MB
会议名称：
第六届动物营养、保健与饲料添加剂国际学术研讨会
会议时间：
2013-7-22
会议地点：
青岛
会议主办单位：
国家生猪产业技术创新战略联盟
会议论文集名称：
第六届动物营养、保健与饲料添加剂国际学术研讨会论文集
关键词：
Monosodium glutamate;plasma free fatty acids;short-chain fatty acids;intestine, GLP-1, leptin
摘要：
Monosodium glutamate (MSG) is currently used as a flavor enhancer in human nutrition.Supplemental glutamate may impact metabolism.In this study,we investigate the effects of three doses of MSG (0.06,0.5 and 1.0 g/kg body weight/day) on several biochemical parameters.Results indicate that although MSG supplementation had little effects on body weight,it dose-dependently decreased serum free fatty acid concentration,and transiently increased the plasma concentrations of leucine,isoleucine,phenylalanine,alanine,glutamate and aspartate.MSG at the highest dose tested increased the plasma GLPl concentration whilst decreasing leptin concentration.In addition,MSG supplementation decreased plasma glutathion-S-transferase activity whilst increasing superoxide dismutase activity.In the colonic luminal content,MSG supplementation was able to increase acetate,propionate and butyrate concentrations.Although small intestine morphology was little affected by MSG supplementation,the expression of the EAACl glutamate transporter was slightly but significantly increased following supplementation with this flavor enhancer.It is concluded that MSG supplementation modifies nutrient plasma concentration,affects hormonal profile as well as plasma concentration of enzymes related to the control of reactive oxygen species.In addition,MSG supplementation increases the short-chain fatty acid colonic luminal concentrations and affect on small intestine mucosa morphology.The consequences of such biochemical changes in this experimental model is discussed.
语种：
英文
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猪源枯草芽孢杆菌的分离鉴定及生物学特性研究
作者：
窦茂鑫;赵迪;王蕾;丁斌鹰;侯永清;...
期刊：
养殖与饲料 ,2012年(4):11-14 ISSN：1671-427X
作者机构：
武汉工业学院动物科学与营养工程学院／动物营养与饲料科学湖北省重点实验室,武汉,430023;[丁斌鹰; 侯永清; 窦茂鑫; 王蕾; 赵迪; 吴涛] 武汉工业学院
关键词：
枯草芽孢杆菌;生物学特性;耐受性;猪粪便;猪微生态饲料添加剂
摘要：
本研究旨在分离、鉴定出经过猪肠道驯化的枯草芽孢杆菌，研究其耐高温、耐酸、耐胆盐、抑菌性等生物学特性，为猪微生态饲料添加剂的研发提供候选菌株。试验以新鲜的猪粪便为材料，通过细菌培养特征、形态观察、生化鉴定、16SrDNA序列分析，分离、鉴定出5株枯草芽孢杆菌；对所鉴定的枯草芽孢杆菌进行高温处理、酸处理和胆盐处理后，比较其耐高温、耐酸、耐胆盐的能力；通过对常见病原菌的抑菌性的测定，比较其抑菌效果。结果表明：5株枯草芽孢杆菌均具有一定的生物学特性，但性能参差不齐，其中BS7菌株整体特性最好。因此该批从猪粪便中分离的枯草芽孢杆菌中BS7菌株具有较好的生物学特性，可以作为猪微生态饲料添加剂的候选菌株。
语种：
中文
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Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide
作者：
Hou, Yongqing* ;Wang, Lei;Zhang, Wei;Yang, Zhenguo;Ding, Binying;...
期刊：
Amino Acids ,2012年43(3):1233-1242 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Zhang, Wei; Hou, Yongqing; Yang, Zhenguo; Wang, Lei; Qiu, Yinsheng; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
N-acetylcysteine;Intestinal functions;Piglets;Lipopolysaccharide
摘要：
The neonatal small intestine is susceptible to damage by endotoxin, but effective methods for prevention and treatment are lacking. N-acetylcysteine (NAC) is a widely used precursor of L-cysteine for animal cells and plays an important role in protecting cells against oxidative stress. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of NAC on intestinal function. Eighteen piglets were randomly allocated into control, LPS and LPS + NAC groups. The control and LPS groups were fed a corn- and soybean meal-based diet, and the LPS + NAC group was fed the basal diet +500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS and LPS + NAC groups received intraperitoneal administration of LPS (100 lg/kg BW), whereas the control piglets received saline. On day 20 of the trial, D-xylose (0.1 g<kg BW) was orally administrated to all piglets 2 h after LPS or saline injection, and blood samples were collected 1 h thereafter. One hour blood xylose test was used to measure intestinal absorption capacity and mucosal integrity, and diamine oxidase (DAO) was used as a marker of intestinal injury. On day 21 of the trial, pigs were killed to obtain the intestinal mucosa. Compared to the control, LPS challenge reduced (P<0.05) the concentrations of D-xylose (a marker of intestinal absorption) in plasma, activities of DAO in the jejunal mucosa, the ratio of villus height to crypt depth in the jejunal mucosa, RNA/DNA and protein/DNA in the jejunal and ileal mucosae, while increasing (P<0.05) DAO activity in plasma and caspase-3 expression in the intestinal mucosa. The adverse effects of LPS were partially ameliorated (P<0.05) by NAC supplementation. Moreover, NAC prevented the LPS-induced decrease in claudin-1 and occludin expression in the jejunal and ileal mucosae. Collectively, these results indicate that dietary NAC supplementation alleviates the mucosal damage and improves the absorptive function of the small intestine in LPS-challenged piglets. © 2011 Springer-Verlag.
语种：
英文
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非必需氨基酸的必需性
作者：
康萍;侯永清;张金凤
期刊：
饲料工业 ,2012年33(4):50-52 ISSN：1001-991X
作者机构：
武汉工业学院动物科学与营养工程学院,430023,武汉;[张金凤] 武汉多宝微生物技术有限公司;[侯永清; 康萍] 武汉工业学院
关键词：
非必需氨基酸;蛋白质资源;世界性
摘要：
蛋白质资源紧缺是一个世界性的问题,我国由于人口众多、资源有限,蛋白质资源缺乏问题尤为严重.按21世纪初期我国种植业可提供的饲料蛋白质资源量预测,缺口在一半以上.因此,如何提高现有蛋白质资源的利用率是缓解我国蛋白质资源短缺的有效途径之一.蛋白质是动物日粮中最为昂贵的,然而动物的生长主要依赖于蛋白质在体组织中的沉积.日粮蛋白转化为动物蛋白需要复杂的生理和生化过程,如消化形成小肽和氨基酸、小肽和氨基酸的吸收、信号转导,以及代谢等等.因此,蛋白质的营养主要是氨基酸在体内的代谢.1传统意义的非必需氨基酸传统意义上,氨基酸分为必需氨基酸和非必需氨基酸两大类.必需氨基酸是体内不能提供其合成所需的碳骨架,或合成不足的氨基酸.而非必需氨基酸是指氨基酸在体内合成的量能满足动物的需要量.因此不论是研究还是实际应用,必需氨基酸的营养都是蛋白质营养的焦点,而非必需氨基酸的营养作用则被忽视,而且这些非必需氨基酸在NRC标准里都没有被考虑.
语种：
中文
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Effects of Oral Administration of Spermine on the Development of Small Intestine and Growth Performance of Weaned Pigs
作者：
Kang, Ping;Wang, Meng;Hou, Yongqing* ;Yin, Yulong;Ding, Binying;...
期刊：
Journal of Animal and Veterinary Advances ,2012年11(15):2782-2787 ISSN：1680-5593
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Qiu, Yinsheng; Wang, Meng; Yin, Yulong; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
D-xylose;Growth performance;Intestinal morphology;Intestinal protein/DNA;Piglets;Spermine
摘要：
The effects of spermine on the development of small intestine and growth performance of newly weaned pigs were investigated. Thirty pigs were used and the treatment of spermine was through oral administration for the 1 st 3 days after weaning with the dose of 0, 300, 600, 900 or 1200 umol per pig daily. The administration of spermine improved Average Daily Feed Intake (ADFI) and Average Daily Gam (ADG) in the periods 0-14 (p<0.05) but hadno significant effects on the ratio of Feed to Gain (F/G) and protein/DNA contents of the duodenal or ileal mucosa. The protein/DNA content of the jejunum was significantly increased at the doses of 300 and 600 umol day"1 spermine but was declined at the higher doses. The villus height in the duodenum (p<0.01) and ileum (p<0.05) increased with an increase in spermine doses. The crypt depth was reduced by spermine in the jejunum and the ratio of villus height/crypt depth was increased by spermine in the duodenum, jejunum and ileum (p<0.05). Additionally, the treatment of spermine at tested doses showed no effect on D-xylose absorption by the small intestine. These results indicate that oral administration of spermine for 3 days after weaning increased ADG and ADFI, enhanced the development of the small intestinal mucosa and improved intestinal morphology. © Medwell Journals, 2012.
语种：
英文
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Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs
作者：
Li, Quan;Liu, Yulan* ;Che, Zhengquan;Zhu, Huiling;Meng, Guoquan;...
期刊：
INNATE IMMUNITY ,2012年18(6):804-814 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Che, Zhengquan; Liu, Yulan; Li, Quan; Meng, Guoquan; Hou, Yongqing; Chen, Feng; Yin, Yulong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yulan; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Arginine;weaned pigs;lipopolysaccharide;Toll-like receptor 4;nuclear factor-kappa B;liver injury
摘要：
This study was conducted to evaluate whether dietary supplementation with L-arginine (Arg) could attenuate Escherichia coli LPS-induced liver injury through the TLR4 signaling pathway in weaned pigs. Eighteen weaned pigs were allotted to three treatments: non-challenged control, LPS challenged control and LPS-+-0.5% Arg. On d 18, pigs were injected with LPS at 100-μg/kg of body weight (BW) or sterile saline. Blood samples were obtained at 4-h post-injection. Pigs were then sacrificed for the collection of liver samples. Arg supplementation (0.5%) alleviated liver morphological impairment, including hepatocyte caryolysis, karyopycnosis and fibroblast proliferation induced by LPS challenge; it mitigated the increase of serum aspartate aminotransferase and alkaline phosphatase activities induced by LPS (P-<-0.05); it prevented the increase of hepatic TNF-α, malondialdehyde contents and mast cell number induced by LPS administration (P-<-0.05); and it attenuated the elevation of hepatic NF-κB and TLR4-positive cell percentages (P-<-0.05). These results indicate that Arg supplementation has beneficial effects in attenuating hepatic morphological and functional injury induced by LPS challenge in piglets. Additionally, it is possible that the protective effects of Arg on the liver are associated with a decreased release of liver pro-inflammatory cytokines and free radicals through inhibiting TLR4 signaling. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
语种：
英文
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