Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide
作者：
Qin, Qin;Xu, Xiao;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;...
期刊：
Scientific Reports ,2018年8(1):15124 ISSN：2045-2322
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Qin, Qin; Xu, Xiao; Liu, Yulan; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Dai, Bing; Liu, Xiuting] Zhe Jiang Goshine Test Technol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
摘要：
This experiment aimed to explore whether glutamate (Glu) had beneficial effects on intestinal injury caused by Escherichia coli LPS challenge via regulating mTOR, TLRs, as well as NODs signaling pathways. Twenty-four piglets were allotted to 4 treatments including: (1) control group; (2) LPS group; (3) LPS + 1.0% Glu group; (4) LPS + 2.0% Glu group. Supplementation with Glu increased jejunal villus height/crypt depth ratio, ileal activities of lactase, maltase and sucrase, and RNA/DNA ratio and protein abundance of claudin-1 in jejunum and ileum. In addition, the piglets fed Glu diets had higher phosphorylated mTOR (Ser 2448 )/total mTOR ratio in jejunum and ileum. Moreover, Glu decreased TNF-α concentration in plasma. Supplementation with Glu also decreased mRNA abundance of jejunal TLR4, MyD88, IRAK1, TRAF6, NOD2 and increased mRNA abundance of ileal Tollip. These results indicate that Glu supplementation may be closely related to maintaining mTOR and inhibiting TLR4 and NOD signaling pathways, and concomitant improvement of intestinal integrity under an inflammatory condition. © 2018, The Author(s).
语种：
英文
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Dietary modulation of endogenous host defense peptide synthesis as an alternative approach to in-feed antibiotics
作者：
Robinson, Kelsy;Ma, Xi;Liu, Yulan;Qiao, Shiyan;Hou, Yongqing;...
期刊：
动物营养(英文) ,2018年4(2):160-169 ISSN：2405-6545
通讯作者：
Zhang, Guolong
作者机构：
[Zhang, Guolong; Robinson, Kelsy] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Qiao, Shiyan; Ma, Xi] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Zhang, Guolong; Liu, Yulan; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhang, Guolong] O;[Zhang, Guolong] W;Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Host defense peptides;Antibiotic alternatives;Butyrate;Vitamin D;Antibiotics
摘要：
Traditionally, antibiotics are included in animal feed at subtherapeutic levels for growth promotion and disease prevention. However, recent links between in-feed antibiotics and a rise in antibiotic-resistant pathogens have led to a ban of all antibiotics in livestock production by the European Union in January 2006 and a removal of medically important antibiotics in animal feeds in the United States in January 2017. An urgent need arises for antibiotic alternatives capable of maintaining animal health and productivity without triggering antimicrobial resistance. Host defense peptides (HDP) are a critical component of the animal innate immune system with direct antimicrobial and immunomodulatory activities. While in-feed supplementation of recombinant or synthetic HDP appears to be effective in maintaining animal performance and alleviating clinical symptoms in the context of disease, dietary modulation of the synthesis of endogenous host defense peptides has emerged as a cost-effective, antibiotic-alternative approach to disease control and prevention. Several different classes of small-molecule compounds have been found capable of promoting HDP synthesis. Among the most efficacious compounds are butyrate and vitamin D. Moreover, butyrate and vitamin D synergize with each other in enhancing HDP synthesis. This review will focus on the regulation of HDP synthesis by butyrate and vitamin D in humans, chickens, pigs, and cattle and argue for potential application of HDP-inducing compounds in antibiotic-free livestock production. © 2018 Chinese Association of Animal Science and Veterinary Medicine
语种：
英文
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Establishment of a porcine model of indomethacin-induced intestinal injury
作者：
Yi, Dan;Liu, Wenkai;Hou, Yongqing* ;Wang, Lei;Zhao, Di;...
期刊：
Frontiers in Bioscience-Landmark ,2018年23(11):2166-2176 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Wenkai; Wu, Tao; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Animal Model;Hematology;Indomethacin;Inflammation;Intestinal injury;Piglets;Review
摘要：
A useful animal model of intestinal injury is pivotal for studying its pathogenesis and developing nutritional interventions (e.g., amino acid supplementation). Here, we propose the use of indomethacin (IDMT) to induce intestinal inflammation in neonatal pigs. Fourteen-day-old piglets fed a milk replacer diet receive intraperitoneal administration of IDMT (5 mg/kg body weight) for 3 consecutive days. On day 4, blood and intestinal samples are obtained for physiological and biochemical analyses. IDMT increases blood DAO activity, I-FABP concentration, neutrophil and eosinophil numbers; intestinal MMP3 mRNA levels, MPO activity, and MDA concentration; but reduces the plasma concentration of citrulline (synthesized exclusively by enterocytes of the small intestine), intestinal GSH-Px activity, and mRNA levels for villin, I-FABP, TRPV6, AQP10, and KCNJ13. Moreover, extensive hemorrhagic spots, thinned intestinal wall, and ulcers in the distal jejunum and ileum are observed in IDMT-challenged piglets. Furthermore, IDMT decreases intestinal villus height and villus surface area in the piglet jejunum. Collectively, this work establishes a porcine model of intestinal injury for designing novel nutritional means to improve gut function in pigs and humans. © 2018 Frontiers in Bioscience. All Rights Reserved.
语种：
英文
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幼龄仔猪PEDV感染肠道损伤模型的建立
作者：
李鹏;吴梦郡;余魁;徐海旺;吴涛;...
期刊：
中国畜牧兽医 ,2018年45(6):1715-1721 ISSN：1671-7236
作者机构：
[余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 易丹; 赵迪; 吴涛] 武汉轻工大学
关键词：
猪流行性腹泻病毒;仔猪;肠道损伤;模型
摘要：
试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组:对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为104.5 TCID50）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1g/kg体重）,1h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明:（1）PEDV感染显著降低了仔猪ADG（P<0.05）,极显著提高了仔猪腹泻率（P<0.01）;（2）PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01）,显著提高了十二指肠和空肠隐窝深度（P<0.05）;（3）PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01）,极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明,口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。
语种：
中文
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Dietary supplementation with an amino acid blend enhances intestinal function in piglets
作者：
Yi, Dan;Li, Baocheng;Hou, Yongqing* ;Wang, Lei;Zhao, Di;...
期刊：
Amino Acids ,2018年50(8):1089-1100 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Li, Baocheng; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Zhou, Ying; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Amino acid blend;Intestinal function;Piglets
摘要：
The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB. On day 20 of the trial, blood and intestinal tissue samples were obtained from piglets. Compared with the control, AAB supplementation reduced (P < 0.05) diarrhoea incidence; plasma alanine aminotransferase and diamine oxidase activities; intestinal concentrations of hydrogen peroxide, malondialdehyde, and heat shock protein-70, and intestinal mRNA levels for interleukin-1β, interferon-γ, and chemokine (C–X–C motif) ligand-9; and the numbers of Enterobacterium family, Enterococcus genus and Clostridium coccoides in the colon digesta. Furthermore, AAB supplementation enhanced (P < 0.05): the plasma concentrations of serine, aspartate, glutamate, cysteine, tyrosine, phenylalanine, tryptophan, lysine, arginine, citrulline, ornithine, taurine, and γ-aminobutyric acid; intestinal villus height and surface area, villus height/crypt depth ratio, antioxidative enzyme activities, and mRNA levels for porcine β-defensin-1, sodium-independent amino acid transporters (b 0,+ AT and y + LAT1), aquaporin (AQP) 3, AQP8, AQP10, nuclear factor erythroid 2-related factor 2 and glutathione S-transferase omega-2, and protein abundances of AQP3, AQP4, claudin-1, occludin and myxovirus resistance 1; and the numbers of Bifidobacterium genus and Lactobacillus genus in the colon digesta. Collectively, these comprehensive results indicate that dietary AAB supplementation plays an important role in improving piglet growth and intestinal function. © 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
语种：
英文
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Baicalin Inhibits Haemophilus Parasuis-Induced High-Mobility Group Box 1 Release during Inflammation
作者：
Fu, Shulin;Liu, Huashan;Chen, Xiao;Qiu, Yinsheng* ;Ye, Chun;...
期刊：
International Journal of Molecular Sciences ,2018年19(5) ISSN：1661-6596
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Huashan; Liu, Yu; Hou, Yongqing; Ye, Chun; Chen, Xiao; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Huashan; Liu, Yu; Hou, Yongqing; Ye, Chun; Chen, Xiao; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
baicalin;Haemophilus parasuis;HMGB1;RNA-Seq;inflammation
摘要：
Haemophilus parasuis (H. parasuis) can cause Glässer’s disease in pigs. However, the molecular mechanism of the inflammation response induced by H. parasuis remains unclear. The high-mobility group box 1 (HMGB1) protein is related to the pathogenesis of various infectious pathogens, but little is known about whether H. parasuis can induce the release of HMGB1 in piglet peripheral blood monocytes. Baicalin displays important anti-inflammatory and anti-microbial activities. In the present study, we investigated whether H. parasuis can trigger the secretion of HMGB1 in piglet peripheral blood monocytes and the anti-inflammatory effect of baicalin on the production of HMGB1 in peripheral blood monocytes induced by H. parasuis during the inflammation response. In addition, host cell responses stimulated by H. parasuis were determined with RNA-Seq. The RNA-Seq results showed that H. parasuis infection provokes the expression of cytokines and the activation of numerous pathways. In addition, baicalin significantly reduced the release of HMGB1 in peripheral blood monocytes induced by H. parasuis. Taken together, our study showed that H. parasuis can induce the release of HMGB1 and baicalin can inhibit HMGB1 secretion in an H. parasuis-induced peripheral blood monocytes model, which may provide a new strategy for preventing the inflammatory disorders induced by H. parasuis. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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三丁酸甘油酯对乙酸刺激仔猪肠道屏障功能的影响
作者：
张俊梅;宋转;周颖;刘文凯;易丹;...
期刊：
中国畜牧兽医 ,2018年45(9):2440-2446 ISSN：1671-7236
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,武汉430023;[侯永清; 张俊梅; 王蕾; 周颖; 易丹; 宋转; 赵迪; 刘文凯] 武汉轻工大学
关键词：
三丁酸甘油酯;乙酸;仔猪;肠道屏障
摘要：
本试验旨在研究三丁酸甘油酯（TB）对乙酸（ACA）刺激仔猪小肠黏膜生长和肠道屏障功能的影响。试验选用18头体重接近、健康的28日龄断奶仔猪（杜×长×大）,随机分为3个处理:对照组、乙酸组和TB组,每个处理6个重复,每个重复1头猪。试验期为21d。试验期内对照组和乙酸组饲喂基础日粮,TB组饲喂基础日粮+0.1%TB。于试验第15天清晨,乙酸组和TB组仔猪直肠灌注10mL 10%乙酸,对照组直肠灌注相应剂量的灭菌生理盐水。于试验第18、21天清晨前腔静脉采血,测定血浆二胺氧化酶（DAO）活力;第21天屠宰取样,取空肠、回肠等组织样品,测定仔猪肠道形态结构、肠道黏膜损伤相关基因mRNA水平等指标。结果表明:（1）未用乙酸刺激前,与对照组相比,日粮中添加0.1%TB对仔猪平均日增重有一定程度提高（P=0.089）,且显著降低了仔猪料重比（P<0.05）;（2）与对照组相比,乙酸组显著降低了空肠黏膜绒毛高度、回肠黏膜绒毛高度与隐窝深度比值及空肠绒毛表面积（P<0.05）;（3）与乙酸组相比,TB组显著降低了血浆DAO活力（P<0.05）,极显著降低了回肠AREG基因的相对表达量（P<0.01）。综合上述结果,日粮中添加0.1%TB能缓解乙酸诱导的仔猪肠黏膜生长及肠道屏障功能的损伤。
语种：
中文
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凝结芽孢杆菌对断奶仔猪生长性能、肠道菌群和血液生化指标的影响
作者：
张越;徐海旺;李鹏;周颖;张俊梅;...
期刊：
黑龙江畜牧兽医 ,2018年(12):165-168 ISSN：1004-7034
作者机构：
武汉轻工大学动物科学与营养工程学院/动物营养与饲料科学湖北省重点实验室,武汉,430023
关键词：
凝结芽孢杆菌;断奶仔猪;生长性能;肠道菌群;血液生化指标
摘要：
为了研究日粮中添加凝结芽孢杆菌对仔猪生长性能、肠道菌群和血液生化指标的影响,试验选取21日龄健康断奶仔猪30头,随机分为3组(对照组、试验Ⅰ组、试验Ⅱ组),对照组饲喂基础日粮,试验Ⅰ组、试验Ⅱ组分别在基础日粮中添加凝结芽孢杆菌2.0× 106 cfu/g、2.0× 107 cfu/g.预试期3d,正试期21 d.于试验第21天采血,屠宰,测定其生长性能、肠道菌群和血液生化指标.结果表明:日粮中添加不同水平的凝结芽孢杆菌均显著降低了断奶仔猪的腹泻率(P＜0.05);提高了仔猪回肠内容物中乳酸菌的数量(P＜0.05),降低了回肠内容物中梭菌的数量(P＜0.05);提高了血浆中磷酸丝氨酸、胱氨酸、酪氨酸等多种氨基酸的含量(P＜0.05),降低了磷酰乙醇铵的含量(P＜0.05).说明在日粮中添加凝结芽孢杆菌可以改善断奶仔猪的肠道菌群,降低仔猪的腹泻率.
语种：
中文
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Nutritionally Essential Amino Acids
作者：
Hou, Yongqing;Wu, Guoyao
期刊：
Advances in Nutrition ,2018年9(6):849-851 ISSN：2161-8313
通讯作者：
Wu, G.
作者机构：
[Hou, Yongqing] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, China;[Wu, Guoyao] Department of Animal Science, Texas A&M University, College Station, TX
通讯机构：
Department of Animal Science, Texas A and M University, College Station, TX, United States
摘要：
Amino acids (AAs) are defined as organic compounds containing both amino and acid groups (1). Proteinogenic AAs are AAs that serve as substrates for protein synthesis in animal cells, and occur naturally as L-AAs except for glycine. Free AAs (those AAs that are not constituents of peptides or polymers) in physiologic fluid exist primarily in an L-isoform, but some of them may be present in a D-isoform. Based on his findings in 1912 that adult dogs exhibited a negative nitrogen balance when fed a tryptophan-free diet but maintained a positive nitrogen balance when fed a proline-free diet, Abderhalden (2) classified AAs as nutritionally essential (EAAs) or nonessential (NEAAs). Beginning in 1924, WC Rose and coworkers (3) published a series of landmark papers on AA nutrition and metabolism in rats and humans that further defined AAs as EAAs or NEAAs based on nitrogen balance or growth. We published an essay on NEAAs in 2017 (4), and EAAs are the focus of the present article.
语种：
英文
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The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide
作者：
Kang, Ping;Liu, Yulan* ;Zhu, Huiling;Zhang, Jing;Shi, Haifeng;...
期刊：
Animal Bioscience ,2018年31(4):548-555 ISSN：2765-0189
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Zhang, Jing; Liu, Yulan; Pi, Dinan; Leng, Weibo; Kang, Ping; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Asparagine;Energy Metabolism;Lipopolysaccharide;Piglets
摘要：
Objective: This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide. Methods: Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected. Results: At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters. Conclusion: The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge. Copyright © 2018 by Asian-Australasian Journal of Animal Sciences.
语种：
英文
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N-乙酰半胱氨酸灌胃对超早期断奶仔猪血浆中游离氨基酸及小肠形态结构与功能的影响
作者：
周佳;李鹏;程强;张俊梅;周颖;...
期刊：
中国畜牧杂志 ,2018年54(4):50-54 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[李鹏; 周佳; 侯永清; 张俊梅; 程强; 王蕾; 周颖; 易丹] 武汉轻工大学
关键词：
N-乙酰半胱氨酸;仔猪;游离氨基酸;小肠
摘要：
本试验旨在研究N-乙酰半胱氨酸(N-acetylcysteine,NAC)灌胃对超早期断奶仔猪血浆中游离氨基酸、小肠形态结构及功能的影响.将22头体重为(2.99±0.41) kg的7日龄断奶仔猪随机分为对照组和NAC组,每组1 1个重复,每个重复1头猪.在饲喂人工乳的基础上,NAC组每天每头按50 mg/kg体重进行NAC灌胃,对照组每天每头进行同体积的生理盐水灌胃.于试验第11天按0.1 g/kg体重的剂量给仔猪灌服D-木糖溶液,1h后前腔静脉采血,然后屠宰取小肠组织样,刮取肠黏膜及制作组织切片.结果表明:与对照组相比,NAC组显著提高了超早期断奶仔猪平均日增重(P＜0.05);提高了超早期断奶仔猪血浆中谷氨酰胺、胱氨酸、γ-氨基丁酸水平(P＜0.05),降低了血浆中尿素氮、半胱氨酸、色氨酸水平和二胺氧化酶活力(P＜0.05):NAC灌胃提高了哺乳仔猪十二指肠黏膜的绒毛高度/隐窝深度(P＜0.05),有降低十二指肠隐窝深度的趋势(P＜0.10),有提高空肠绒毛宽度和回肠绒毛高度的趋势(P＞0.10).在本试验条件下,NAC灌胃可改善小肠形态结构与屏障功能,提高氨基酸的利用率,调节血浆中部分氨基酸的含量.
语种：
中文
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复合植物精油对脂多糖刺激仔猪肝脏的保护作用
作者：
李鹏;汪中兴;吴梦郡;余魁;纪昌正;...
期刊：
中国畜牧杂志 ,2018年54(3):87-91 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023
关键词：
复合植物精油;脂多糖;仔猪;肝脏
摘要：
试验旨在研究复合植物精油(OCT)对脂多糖(LPS)刺激仔猪肝脏的保护作用.选取18头仔猪,随机分为对照组、LPS组和OCT+LPS组,每组6个重复.对照组与LPS组饲喂基础日粮,OCT+LPS组在基础日粮中添加50 mg/kg OCT.试验期21 d.于试验第21天,LPS组与OCT+LPS组仔猪腹腔注射LPS,对照组注射等量的生理盐水,3h后采血,6h后屠宰.结果表明:与对照组相比,LPS刺激提高了血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷酰转肽酶(GGT)、肝脏诱导型一氧化氮合酶(i-NOS)的活力(P＜0.05),肝脏热休克蛋白70 (HSP70)基因的相对表达量显著提高(P＜0.05);仔猪肝脏表皮生长因子(EGF)、白细胞介素10 (IL-10)、胰岛素样因子(IGF-1)、丝氨酸蛋白激酶(mTOR)基因的相对表达量显著降低(P＜0.05).在日粮中添加50 mg/kg的OCT,可缓解LPS导致的仔猪血浆ALT、AST、GGT活力的升高以及肝脏i-NOS活力的升高(P＜0.05),且有缓解LPS导致的仔猪肝脏MPO活力升高的趋势(P＜0.1),另外可缓解LPS导致的仔猪肝脏HSP70基因相对表达量的升高以及EGF、IL-10、IGF-1、mTOR等基因相对表达量的降低(P＜0.05).综上可知,日粮中添加50 mg/kg的OCT可缓解LPS刺激引起的肝脏炎症反应,提高仔猪肝脏细胞的生长和增殖,进而缓解LPS刺激导致的仔猪肝脏氧化应激损伤.
语种：
中文
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Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis: New Insights into Pathogenesis
作者：
Fu, Shulin;Guo, Jing;Li, Ruizhi;Qiu, Yinsheng* ;Ye, Chun;...
期刊：
International Journal of Molecular Sciences ,2018年19(5) ISSN：1422-0067
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Li, Ruizhi; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Li, Ruizhi; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Haemophilus parasuis;Infection;Porcine aortic vascular endothelial cells;Vascular damage
摘要：
Haemophilus parasuis is the causative agent of Glässer’s disease in pigs. H. parasuis can cause vascular damage, although the mechanism remains unclear. In this study, we investigated the host cell responses involved in the molecular pathway interactions in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis using RNA-Seq. The transcriptome results showed that when PAVECs were infected with H. parasuis for 24 h, 281 differentially expressed genes (DEGs) were identified; of which, 236 were upregulated and 45 downregulated. The 281 DEGs were involved in 136 KEGG signaling pathways that were organismal systems, environmental information processing, metabolism, cellular processes, and genetic information processing. The main pathways were the Rap1, FoxO, and PI3K/Akt signaling pathways, and the overexpressed genes were determined and verified by quantitative reverse transcription polymerase chain reaction. In addition, 252 genes were clustered into biological processes, molecular processes, and cellular components. Our study provides new insights for understanding the interaction between bacterial and host cells, and analyzed, in detail, the possible mechanisms that lead to vascular damage induced by H. parasuis. This may lead to development of novel therapeutic targets to control H. parasuis infection. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响
作者：
徐海旺;余魁;吴梦郡;刘文凯;李鹏;...
期刊：
中国畜牧兽医 ,2018年45(8):2197-2203 ISSN：1671-7236
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉 430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心 ,武汉430023;[余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 易丹; 刘文凯] 武汉轻工大学
关键词：
复合植物精油(OCT);断奶仔猪;脂多糖(LPS);免疫应激
摘要：
试验旨在研究复合植物精油(OC T)对脂多糖(L PS)诱导的断奶仔猪的免疫应激的影响.试验选取28日龄左右的杜×长×大三元杂交仔猪,随机分成3个处理组:对照组(基础日粮,注射生理盐水),L PS组(基础日粮,注射LPS),OCT组(基础日粮+50 mg/kg OCT,注射LPS).每组8个重复,每个重复1头猪.于试验第21天注射L PS(100μg/kg体重)或等体积的生理盐水,注射3 h后前腔静脉采血进行白细胞分类计数;6 h后屠宰,取胸腺、脾脏,-80℃保存,用于测量胸腺、脾脏免疫相关基因白介素-4(IL-4)、白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子(TNF-α)、核因子(NF-κB)以及转录相关基因转录激活因子3(STAT3)的基因相对表达量.结果表明:①与对照组相比,L PS刺激降低了仔猪血液中白细胞、中性粒细胞、淋巴细胞、单核细胞及嗜酸性粒细胞数量(P<0.05);与LPS组相比,日粮中添加OCT提高了白细胞的数量(P<0.05);②与对照组相比,LPS刺激上调了脾脏、胸腺IL-6、STAT3 mRNA水平(P<0.05),下调了脾脏IL-4、TNF-α及胸腺IL-4、TNF-α、NF-κB mRNA水平(P<0.05);与LPS组相比,添加OCT下调了脾脏、胸腺IL-6、STAT3 mRNA水平(P<0.05);上调了胸腺IL-4、IL-10、TNF-α、NF-κB的mRNA水平(P<0.05).综合上述结果,LPS诱导发生了仔猪免疫应激,OCT在一定程度具有缓解作用.
语种：
中文
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Injury and mechanism of recombinant E-coli expressing STa on piglets colon
作者：
Lv, Yang;Li, Xueni;Zhang, Lin;Shi, Yutao;Du, Linxiao;...
期刊：
Journal of Veterinary Medical Science ,2018年80(2):205-212 ISSN：0916-7250
通讯作者：
Wu, Tao
作者机构：
[Wu, Tao; Gong, Joshua; Lv, Yang; Hou, Yongqing; Li, Xueni; Shi, Yutao; Ding, Binying; Du, Linxiao] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构：
[Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
colon;heat-stable enterotoxin A;piglet;recombinant escherichia coli
摘要：
Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 × 109 CFU E. coli LMG194-STa), LMG194 group (2 × 109 CFU E. coli LMG194) and K88 group (2 × 109 CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H2O2; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines.
语种：
英文
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N-乙酰半胱氨酸对乙酸刺激仔猪肠黏膜生长及肠道屏障功能的影响
作者：
周颖;宋转;张俊梅;刘文凯;余魁;...
期刊：
中国饲料 ,2018年(15):48-53 ISSN：1004-3314
作者机构：
1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室;2. 动物营养与饲料安全湖北省协同创新中心
关键词：
N-乙酰半胱氨酸;乙酸;仔猪;肠黏膜
摘要：
为研究N-乙酰半胱氨酸（NAC）对乙酸（ACA）刺激仔猪肠黏膜屏障功能以及肠道黏膜生长的影响,试验选取体重相似且体况健康的仔猪18头,随机分为对照组、ACA组和NAC组,其中前两组饲喂基础饲粮,NAC组在基础饲粮中添加0.05%NAC,每组6个重复,每个重复1头猪。试验第15天对ACA组和NAC组仔猪直肠灌注10 m L10%的乙酸,对照组灌注相应剂量的生理盐水。第21天灌服D-木糖溶液后采集前腔静脉血样,深度麻醉后屠宰,取小肠黏膜样品待测。结果表明:与对照组相比,ACA组仔猪血浆二胺氧化酶（DAO）活性、十二指肠和回肠的隐窝深度分别提高了94.80%、14.31%、8.14%（P<0.05）;十二指肠DAO活性降低了17.95%（P<0.05）,空肠绒毛高度、十二指肠和回肠绒毛高度/隐窝深度比值,十二指肠和空肠绒毛表面积,及回肠RNA/DNA比值和TP/DNA比值分别降低了14.03%、26.72%、14.97%、12.40%、37.11%、38.91%、26.35%（P<0.05）。饲粮中添加NAC可以缓解以上这些负面影响。此外,相较于ACA组,NAC血浆中D-木糖和表皮生长因子（EGF）含量以及回肠中EGF m RNA水平提高了17.29%、26.20%、34.03%（P<0.05）。综上所述,NAC能够改善ACA刺激造成的仔猪小肠黏膜屏障功能受损,NAC可能是通过提高EGF含量以及EGF m RNA水平,促进细胞生长,修复肠道损伤。
语种：
中文
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Effects of dietary coated-oleum cinnamomi supplementation on the immunity and intestinal integrity of broiler chickens
作者：
Guo, Shuangshuang;Cheng, Qiang;Li, Yehan;Duan, Rui;Hou, Yongqing;...
期刊：
Animal Science Journal ,2018年89(11):1581-1590 ISSN：1344-3941
通讯作者：
Ding, Binying
作者机构：
[Cheng, Qiang; Duan, Rui; Guo, Shuangshuang; Li, Yehan; Hou, Yongqing; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Ding, Binying] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
broiler chicken;coated oleum cinnamomi;immunity;intestinal integrity
摘要：
Oleum cinnamomi (OCM) is increasingly used as a feed additive in animal diets. The aim of this study was to investigate the effects of dietary supplementation with coated‐OCM (cOCM) on the immunity and intestinal integrity of broiler chickens. A total of 396 one‐day‐old chicks were randomly assigned into six groups. The basal diets were supplemented with 50 mg/kg of flavomycin (positive control) as well as 0 (control), 50, 100, 200, and 300 mg/kg of cOCM. Compared with the control, both positive control and cOCM treatments did not improve the growth performance. Serum immunoglobulin (Ig) Y levels were decreased by flavomycin and 50 mg/kg of cOCM treatments (p < 0.05). Dietary cOCM decreased ileal secretory IgA contents at d 21 and commonly down‐regulated duodenal and ileal mRNA expression of interleukin (IL)‐1β and IL‐8 at d 42 (p < 0.05). The 300 mg/kg of cOCM increased jejunal ratio of villus height to crypt depth and upregulated intestinal claudin‐1 expression (p < 0.05). Jejunal (at d 21) and duodenal (at d 42) mucin‐2 expression was up and downregulated by both 50 and 300 mg/kg of cOCM, respectively (p < 0.05). In conclusion, dietary cOCM addition helped to maintain noninflammatory states of humoral and mucosal immunity, and improved the intestinal integrity of broiler chickens.
语种：
英文
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Dietary Supplementation with Oleum Cinnamomi Improves Intestinal Functions in Piglets
作者：
Yi, Dan;Fang, Qiuhong;Hou, Yongqing* ;Wang, Lei;Xu, Haiwang;...
期刊：
International Journal of Molecular Sciences ,2018年19(5) ISSN：1422-0067
通讯作者：
Hou, Yongqing
作者机构：
[Fang, Qiuhong; Wu, Tao; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Yi, Dan; Xu, Haiwang] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Growth performance;Intestinal function;Oleum cinnamomi;Piglets
摘要：
The present study was to determine the efficacy of dietary supplementation with oleum cinnamomi (OCM) on growth performance and intestinal functions in piglets. Sixteen piglets (24-day-old) were randomly assigned to the control or OCM groups. Piglets in the control group were fed a basal diet, whereas piglets in the OCM group were fed the basal diet supplemented with 50 mg/kg OCM. On day 20 of the trial, blood samples and intestinal tissues were obtained from piglets. Compared with the control group, dietary OCM supplementation increased (p < 0.05) average daily feed intake, plasma insulin levels, villus width and villous surface area in the duodenum and jejunum, DNA levels and RNA/DNA ratios in the ileum, the abundance of Enterococcus genus and Lactobacillus genus in caecum digesta, mRNA levels for epithelial growth factor receptor (EGFR), Ras, extracellular signal-regulated kinase 1/2 (Erk1/2), b-cell lymphoma-extra large (Bcl-xL), villin, junctional adhesion molecule A (JAM-A), myxovirus resistance (MX) 1, MX2 and regenerating islet-derived protein 3 gamma (REG3G), and protein abundances of Ras and claudin-1, but decreased (p < 0.05) diarrhoea incidence; the abundances of Enterobacteriaceae family, Enterococcus genus, Lactobacillus genus, Bifidobacterium genus, and Clostrium coccoides in the colon digesta, and AMP-activated protein kinase (AMPK) mRNA levels and caspase-3 protein abundance in the jejunal mucosa of piglets. Taken together, these data indicate that dietary OCM supplementation modulates intestinal microbiota and improves intestinal function in weanling pigs. OCM is an effective feed additive and alternative to feed antibiotics for improving intestinal health in swine.
语种：
英文
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蛋白质水解物在动物营养中的作用
作者：
侯永清;武振龙;王根虎;戴兆来;伍国耀
期刊：
中国饲料 ,2018年(03):82-90 ISSN：1004-3314
作者机构：
动物营养与饲料科学湖北省重点实验室动物营养与饲料安全湖北省协同创新中心武汉轻工大学;动物营养学国家重点实验室中国农业大学动物科技学院;上海亘泰实业集团;美国得克萨斯农工大学动物科学系
关键词：
动物;营养;蛋白水解物;多肽;持续性
摘要：
近年来,多肽在动物营养中发挥的作用受到广泛关注。动物副产品或植物性蛋白质饲料通过化学、酶或者微生物水解可获得优质的多肽,这些多肽对于家畜、禽类和鱼类的生长具有重要的营养和生理调节作用。日粮中的蛋白质在胃肠道多种酶的作用下也可产生多种肽类,其种类因动物的生理状况和日粮组成的不同而有较大差异。分子量较大的多肽在小肠中会被进一步水解为小肽,并以二肽或三肽的形式被肠上皮细胞吸收。因此,小肽在动物小肠的吸收效率要高于游离氨基酸,更有利于血液中氨基酸的平衡。一些动植物来源的多肽除具有蛋白质营养价值外,还具有抗菌、抗氧化、抗高血压和免疫调节等生物学功能,被称为生物活性肽。生物活性肽通常由2~20个氨基酸组成(有些活性肽氨基酸组成可能超过20个)。在玉米-大豆基础日粮中添加2%~8%的动物蛋白质水解物(如猪小肠、猪黏膜、鲑鱼内脏或家禽组织水解产物)或大豆蛋白质水解产物能够提高断奶仔猪、牛犊、家禽和鱼的生长性能和饲料转化率。因此,在日粮中添加蛋白质水解物能够提高家畜和宠物的营养水平和生长,并改善其健康状况(尤其是肠道健康)及动物福利。
语种：
中文
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Establishment of a recombinant Escherichia coli-induced piglet diarrhea model
作者：
Wu, Tao;Lv, Yang;Li, Xueni;Zhao, Di;Yi, Dan;...
期刊：
Frontiers in Bioscience-Landmark ,2018年23(8):1517-1534 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Gong, Joshua; Lv, Yang; Hou, Yongqing; Wang, Lei; Li, Xueni; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Intestinal injury;Piglet Diarrhea;Recombinant Escherichia coli;Review
摘要：
Enterotoxigenic Escherichia coli strains induce human and animal intestinal dysfunction and injury, and cause diarrhea in weanling pigs. A recombinant E.coli strain LMG194-STa which expressed single toxin STa of ETEC was constructed by directly inserting the STa gene of ETEC into the expression vector pBAD202 and then transferring the recombinant plasmid pBAD-STa into the E.coli LMG19 host strain. Diarrhea and intestinal injury in piglets were induced by oral administration of recombinant strain LMG194-Sta. In vitro, the recombinant strain LMG194-Sta had the same toxicity to the IPEC cell as wild type strain K88, and higher toxicity than the host strain LMG194. In vivo, LMG194-STa caused severe diarrhea in piglets as K88, the diarrhea rate of LMG194-STa and K88 groups was higher than that in the LMG194 and control groups. Both K88 and LMG194-STa induced intestinal inflammation, injury, and atrophy, while adversely affecting the expression of genes for cytokines, transporters and ion channels, and nutrient metabolism. Thus, we established a porcine model of recombinant E. coli strain LMG194-STa-induced diarrhea for future nutritional and mechanistic studies of intestinal dysfunction. © 2018 Frontiers in Bioscience. All Rights Reserved.
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