作者： 刘立鹤;刘军;黄峰;余登航

期刊： 武汉轻工大学学报,2019年38(2):115-121 ISSN：2095-7386

作者机构： 武汉轻工大学动物科学与营养工程学院,湖北武汉,430023;[刘军; 余登航; 黄峰; 刘立鹤] 武汉轻工大学

关键词： 荆楚卓越人才计划;长效机制;协同培养型;水产专业人才培养

摘要： 在水产养殖产品升级与经营转型的大背景下,借我校水产养殖专业列入湖北省“荆楚卓越人才”协同育人计划的契机,针对水产养殖专业人才培养普遍存在的问题,结合我校水产专业人才培养的现状、目标定位与特色发展方向,笔者以教学一线教师的视角,从构建战略联盟长效机制的前提与基础、战略联盟的确定与遴选、人才培养知识体系的构建、专业技能及综合素质培养体系构建等方面,提出自己对“荆楚卓越水产人才”协同培养的一些思考与建议,以期为本校水产专业的学科建设和人才培养提供思路与探讨主题,并通过逐步教学实践探索,进而形成一套专业特色鲜明,能充分适应社会和行业需求的人才培养模式.

语种： 中文

作者： Kong, Long;Cheng, Shi-yan;Xiang, Xiao-jun;Liu, Wen-shu;Yu, Deng-hang;...

期刊： Fish & Shellfish Immunology,2019年86:536-548 ISSN：1050-4648

通讯作者： Dong, Gui-Fang

作者机构： [Cheng, Shi-yan; Kong, Long; Huang, Feng; Dong, Gui-Fang; Xiang, Xiao-jun; Yu, Deng-hang] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Wen-shu] Jiangxi Acad Agr Sci, Inst Anim Husb & Vet, Nanchang 330200, Jiangxi, Peoples R China.;[Yang, Yan-ou] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.;[Zhou, Jiancheng] Wuhan DBN Aquaculture Technol Co Ltd, Wuhan 430090, Hubei, Peoples R China.

通讯机构： [Dong, Gui-Fang] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Conjugated linoleic acid;Ctenopharyngodon idella;Immune responses;Intestinal morphology;TLR4/NF-kappaB signaling pathway

摘要： Conjugated linoleic acid (CLA) has been shown to exhibit anti-inflammatory properties in the intestine in mammals. However, the effect of CLA on intestinal immune response in fish is still unknown. Therefore, a 65-day growth trial was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) on morphology, selective immune parameters, and gene expressions in the intestine of grass carp. Seven isonitrogenous and isolipidic diets were formulated as follows: 0 (control), 0.5 (CLA0.5), 1 (CLA1), 1.5 (CLA1.5), 2 (CLA2), 2.5 (CLA2.5), and 3 (CLA3) g CLA per 100g of feed. RESULTS: showed that dietary supplementation of 1.5-3% CLA significantly (P<0.05) increased the fold and enterocyte heights in the PI and MI of grass carp. Complement 3 (C3) and immunoglobulin M (IgM) contents in three intestinal segments were significantly (P<0.05) higher in fish fed with CLA1.5 to CLA2.5 diets compared to fish fed the control diet. CLA1.5 to CLA2.5 diets significantly (P<0.05) increased the mRNA expression levels of anti-inflammatory cytokines (IL-10 and TGFbeta1) and significantly (P<0.05) reduced the mRNA expression levels of pro-inflammatory cytokines (IL-1beta, IL-8, and TNF-alpha) in the PI, MI, and DI. This improved expression of anti-inflammatory cytokines and the inhibited expression of pro-inflammatory cytokines in the intestine of grass carp, might be mediated via TLR4/NF-kappaB-signaling pathway. Our results suggested that CLA1.5 to CLA2 diets improved intestinal morphology, increased the expression of anti-inflammatory cytokines, and inhibited the expression of pro-inflammatory cytokines in the intestine of grass carp. In conclusion, dietary supplementation of 1.5%-2% CLA show the anti-inflammatory therapeutic potential in the intestine of grass carp. The anti-inflammatory therapeutic potential of CLA might be mediated via TLR4/NF-kappaB-signaling pathway.

语种： 英文

作者： Zhou, Jianjun;Gao, Zhen;Wang, Wenbiao;Huang, Feng;Hu, Junpeng;...

期刊： Aquaculture International,2019年27(6):1655-1665 ISSN：0967-6120

通讯作者： Hu, Xianqin;Wang, Xuedong

作者机构： [Hu, Xianqin; Hu, XQ; Wang, XD; Gao, Zhen; Huang, Feng; Wang, Wenbiao; Yang, Wumin; Zhou, Jianjun; Wang, Rui; Wang, Xuedong] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn,Hubei Key Lab Anim Nutr, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Engn Res Ctr Feed Prot Resources Agr By Prod,Mini, Wuhan 430023, Hubei, Peoples R China.;[Gong, Aqiong; Hu, Junpeng] Angel Yeast Co Ltd, Yichang City 443003, Hubei, Peoples R China.;[Li, Jie] Dong Xihu Farm Adm Hubei Prov, Agr Comprehens Serv Stn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Hu, XQ; Wang, XD] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn,Hubei Key Lab Anim Nutr, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Engn Res Ctr Feed Prot Resources Agr By Prod,Mini, Wuhan 430023, Hubei, Peoples R China.

关键词： Yeast cell wall;Channel catfish;Head-kidney macrophage;Respiratory burst;Phagocytosis

摘要： Yeast cell wall (YCW) preparations are important nutritional additives in the aquaculture industry. Head-kidney macrophages play important roles in innate immune functions in fish. However, the mechanisms underlying their stimulation are not yet characterized. The purpose of this study was to investigate the effects of YCW on immunological functions of channel catfish (Ictalurus punctatus) head-kidney macrophages. Our results showed that YCW preparations not only improved the proliferation and phagocytosis of macrophages but also induced the secretion of reactive oxygen species (ROS), nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta). At a concentration of 500.0 mu g/mL, YCW preparations stimulated macrophage proliferation and phagocytosis to 1.98 and 1.8 times, respectively, when compared with the negative group. Equally, YCW preparations raised the production of NO by inducing iNOS and ROS in a concentration-dependent manner. Furthermore, these preparations enhanced TNF-alpha and IL-1 beta mRNA levels to multiple digits from 1.2 to 3.0 times, respectively. In conclusion, the stimulatory effects of YCW on the phagocytic activity of the fish macrophages were associated with respiratory bursts coupled with nitric oxide production.

语种： 英文

作者： 刘军;刘立鹤;余登航;黄峰;李娇;...

期刊： 武汉轻工大学学报,2019年38(5):103-107 ISSN：2095-7386

作者机构： 武汉轻工大学 动物科学与营养工程学院,湖北 武汉,430023;[刘军; 刘辉宇; 李娇; 余登航; 黄峰; 刘立鹤] 武汉轻工大学

关键词： 水产养殖专业;渔业资源调查;实践教学

摘要： 根据新形势下应用型人才培养的需求,阐述了《渔业资源调查》实习课程的设立缘由。依据武汉轻工大学水产养殖本科人才培养方案,结合课程教学进程安排,探索、设计了《渔业资源调查》实习课程的实施时间、实施地点、教学内容、实习方式、考核内容与考核办法,探讨了实习期间的教学手段。

语种： 中文

作者： 孔龙;邹琦;程时焰;杨严鸥;余登航;...

期刊： 水生生物学报,2019年43(4):708-722 ISSN：1000-3207

通讯作者： Dong, G.-F.

作者机构： 武汉轻工大学动物科学与营养工程学院, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;淡水水产健康养殖湖北省协同创新中心, 淡水水产健康养殖湖北省协同创新中心, 武汉, 430070;安徽农业大学动物科技学院, 合肥, 230036;[董桂芳; 邹琦; 孔龙; 余登航; 程时焰; 黄峰] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China, Freshwater Aquaculture Collaborative Innovation Center in Hubei Province, Wuhan, 430070, China;[杨严鸥] College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China

通讯机构： [Dong, G.-F.] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, China

关键词： 草鱼;共轭亚油酸;细胞学形态;抗氧化能力;基因表达

摘要： 采用65d的生长实验研究共轭亚油酸（CLA）对草鱼肝脏和肌肉细胞学形态、抗氧化能力以及脂肪代谢相关基因表达的影响。共配制7种近似等氮（粗蛋白:36 g/100 g）等脂（粗脂肪:4.5 g/100 g）的饲料:0 （对照组）、0.5 （CLA0.5）、1 （CLA1）、1.5 （CLA1.5）、2 （CLA2）、2.5 （CLA2.5）和3 g/100 g CLA （CLA3）。每个饲料组均设置3个生物学重复,实验鱼初始体重约为（5.08±0.08） g。实验结果显示:与对照组相比, CLA0.5-CLA2.5组草鱼肝脏和肌肉组织细胞学形态均无明显异常,但CLA3组肝细胞线粒体空泡化,内质网肿胀,排列过于紧密;肌细胞肌节结构松散,肌原纤维降解;在CLA1.5-CLA2.5组肝脏和肌肉中超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）和谷胱甘肽过氧化物酶（GPx）活力均显著上升（P<0.05）;在CLA1.5-CLA3组中肝脏过氧化氢酶（CAT）活力均显著高于对照组（P<0.05）,但谷胱甘肽还原酶（GR）活力无显著变化（P>0.05）,且在CLA0.5-CLA3组中肌肉CAT和GR活力均无显著变化（P>0.05）。CLA1.5-CLA2组肝脏中MDA含量显著低于对照组（P<0.05）,而CLA2.5-CLA3组MDA含量显著高于对照组（P<0.05）。CLA3组肌肉中MDA含量显著高于对照组（P<0.05）。与对照组相比,在CLA1.5-CLA2.5组肝脏和肌肉中乙酰辅酶A羧化酶（ACC） mRNA表达显著下调（P<0.05）,脂蛋白脂酶（LPL）、激素敏感性脂肪酶（HSL）和过氧化物酶体增殖物激活受体α（PPARα）均显著上调表达（P<0.05）。在CLA2-CLA3组肝脏以及CLA1.5-CLA2和CLA3组肌肉中脂肪酸去饱和酶2（FAD2）mRNA显著上调表达（P<0.05）。过氧化物酶体增殖物激活受体γ（PPARγ）mRNA在CLA1-CLA3组肝脏中显著下调表达（P<0.05）,而其在肌肉中均无显著变化（P>0.05）。综上所述,结合2%CLA在不影响草鱼的生长和饲料利用,且显著降低肝脏脂肪积累,研究结果建议, 2%CLA在不影响草鱼肝脏和肌肉组织细胞学形态的前提下,可有效提高其肝脏和肌肉组织抗氧化能力并促进其脂肪分解代谢以及抑制脂肪合成代谢相关基因表达。

语种： 中文

作者： Li, Yunxia;Tang, Zhiru;Li, Tiejun;Chen, C.;Huang, Feiruo;...

期刊： JOURNAL OF ANIMAL SCIENCE,2018年96(9):3804-3814 ISSN：0021-8812

通讯作者： Sun, Zhihong

作者机构： [Li, Yunxia] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.;[An, Rui; Sun, Jiajing; Zhen, Jifu; Wu, Zhaoliang; Li, Mao; Wu, Liuting; Sun, Zhihong; Li, Yunxia; Tang, Zhiru; Xu, Qingqing; Zhang, Xiangxin; Yang, Jing; Chen, Jinchao; Chen, C.] Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Huang, Feiruo] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Sun, Zhihong] S;Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： alanine;amino acid;glutamic acid;nitrogen;pyruvic acid;urea;animal;animal food;cell line;cytology;diet;dietary supplement;drug effect;energy metabolism;epithelium cell;intestine mucosa;male;metabolism;oxidation reduction reaction;physiology;pig;protein intake;protein restriction;veterinary medicine;Alanine;Amino Acids;Animal Feed;Animal Nutritional Physiological Phenomena;Animals;Cell Line;Diet;Diet, Protein-Restricted;Dietary Proteins;Dietary Supplements;Energy Metabolism;Epithelial Cells;Glutamic Acid;Intestinal Mucosa;Male;Nitrogen;Oxidation-Reduction;Pyruvic Acid;Swine;Urea

摘要： This study was performed to determine if pyruvate, which acts as a critical intermediate in energy metabolism, can substitute the role of glutamate as a metabolic fuel and effectively reduce nitrogen excretion in pigs. First, the experiment in vitro was carried out to investigate the effects of culturing porcine small intestinal epithelial cell line with pyruvate on the oxidation. Then, barrows weighing 40 kg were used in the experiment investigating the changes of nitrogen balance in response to addition of pyruvate to low-protein diets. Last, barrows (40 kg), which were surgically fitted with permanent catheters in the mesenteric vein, portal vein, hepatic vein, and carotid artery, were used to investigate the effects of supplementing low-protein diets with calcium pyruvate on the net portal fluxes of amino acids (AAs) and the consumption of AAs in the liver. The results showed that culturing cells with sodium pyruvate significantly reduced the number of glutamate oxidation (P < 0.05). Addition of calcium pyruvate to low-protein diets significantly reduced urinary nitrogen excretion from 13.2 g/d (18.0% crude protein, CP) to 10.3 g/d (15.0% CP) or 7.80 g/d (13.5% CP) and total nitrogen excretion from 22.5 g/d (18.0% CP) to 17.8 g/d (15.0% CP) or 14.2 g/d (13.5% CP) (P < 0.05), without obviously negative effects on the nitrogen retention (P > 0.05). Addition of calcium pyruvate to low-protein diets significantly decreased essential AA consumption rate in the liver (P < 0.05). This diet modification reduced the net portal fluxes of NH3, glycine, and alanine, as well as urea production rate in the liver (P < 0.05). The results indicated that pyruvate is an effective substitute for glutamate as a supplement in low-protein diets, reducing porcine nitrogen excretion and nitrogen consumption. © The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.

语种： 英文

作者： Wu, Liuting;Zhang, Xiangxin;Tang, Zhiru;Li, Yunxia;Li, Tiejun;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018年66(31):8262-8271 ISSN：0021-8561

通讯作者： Sun, Zhihong;Yin, Yulong;Jiang, Qingyan;Zhu, Weiyun

作者机构： [An, Rui; Sun, Jiajing; Zhen, Jifu; Wu, Zhaoliang; Li, Mao; Wu, Liuting; Sun, Zhihong; Tang, Zhiru; Xu, Qingqing; Zhang, Xiangxin; Chen, Cheng; Yang, Jing; Chen, Jinchao] Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Li, Yunxia] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.;[Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Huang, Feiruo] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Sun, Zhihong] S;[Yin, Yulong] C;[Jiang, Qingyan] H;[Zhu, Weiyun] N;Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： Ascorbic acid;Liver;Metabolism;Nitrogen;Proteins;Urea;Carotid artery;Crude proteins;Hepatic veins;Low protein diet;Nitrogen balance;Nitrogen excretion;Nitrogen retention;Urea production;Amino acids;amino acid;lysine;methionine;nitrogen;threonine;tryptophan;urea;animal;chemistry;diet;dietary supplement;feces;liver;male;metabolism;pig;protein restriction;urine;veterinary;viscera;Amino Acids;Animals;Diet;Diet, Protein-Restricted;Dietary Supplements;Feces;Liver;Lysine;Male;Methionine;Nitrogen;Sus scrofa;Threonine;Tryptophan;Urea;Viscera

摘要： Reducing dietary crude protein (CP) intake effectively decreases nitrogen excretion in growing-finishing pigs but at the expense of poor growth when dietary CP content is reduced by ≥3%. In this study, we investigated the main disadvantages of low-protein diets supplemented with lysine, methionine, threonine, and tryptophan in pigs. First, changes in the nitrogen balance in response to differences in dietary CP content (18%, 15%, and 13.5%) were investigated in barrows (40 kg). Then, barrows (40 kg) surgically fitted with catheters in the mesenteric vein, portal vein, hepatic vein, and carotid artery were used to investigate changes in amino acid (AA) metabolism in the portal-drained viscera and liver in response to differences in dietary CP content. The results showed that low-protein diets reduced fecal and urinary nitrogen excretion (P &lt; 0.05) meanwhile resulted in significant decreases in nitrogen retention (P &lt; 0.05). Moreover, a reduction in the dietary CP content from 18% to 13.5% resulted in decreases in the net portal fluxes of NH3, glycine, and alanine as well as in the urea production in the liver (P &lt; 0.05), whereas their values as a percentage of nitrogen intake did not decline (P &gt; 0.05). The net portal fluxes of nonessential AA (NEAA) were reduced in the low-protein diet groups (P &lt; 0.05), while essential AA consumption in the liver increased (P &lt; 0.05). Thus, low-protein diets result in reductions in both nitrogen excretion and retention, and NEAA deficiency may be a major disadvantage of low-protein diets. © 2018 American Chemical Society.

语种： 英文

作者： Zou, Qi;Yang, Yan-ou;Wei, Bang-Hong;Yu, Deng-hang;Chen, Lu;...

期刊： Aquaculture Nutrition,2018年24(6):1752-1768 ISSN：1353-5773

通讯作者： Dong, Gui-Fang

作者机构： [Huang, Feng; Wei, Bang-Hong; Zhou, Teng; Dong, Gui-Fang; Zou, Qi; Yu, Deng-hang] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Huang, Feng; Dong, Gui-Fang; Zou, Qi; Yu, Deng-hang] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Hubei, Peoples R China.;[Yang, Yan-ou; Chen, Lu] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei, Anhui, Peoples R China.

通讯机构： [Dong, Gui-Fang] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： adipocytokine;conjugated linoleic acid;Ctenopharyngodon idella;gene expression;growth performance

摘要： A 65‐day growth trial was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) on growth performance, tissue adipocytokine levels and lipid metabolism of grass carp. Seven isonitrogenous and isolipidic diets were formulated as follows: 0 (control), 5 (CLA5), 10 (CLA10), 15 (CLA15), 20 (CLA20), 25 (CLA25) and 30 (CLA30) g/kg CLA. Results showed that incorporating as low as 25 g/kg CLA in the diet significantly decreased the growth of grass carp. The liver lipid content in fish fed with CLA15 to CLA30 significantly decreased. The leptin and resistin levels in liver of fish fed with CLA5 to CLA30 diets significantly increased. The mRNA expression levels of fatty acid synthase (FAS) and triacylglycerol lipase (TGL) in liver of fish fed with CLA5 to CLA30 and CLA10 to CLA30 diets also significantly decreased, respectively. In summary, the lipid‐lowering effects in grass carp liver were induced by the supplementation of 15–20 g/kg CLA, but its growth remained unaffected. Moreover, the lipid‐lowering effects of CLA on grass carp could also be modulated both by adipocytokine levels and by the expression of genes involved in lipid metabolism in tissues.

语种： 英文

作者： Rui An;Tang, Zhiru;Li, Yunxia;Li, Tiejun;Xu, Qingqing;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018年66(15):3793-3800 ISSN：0021-8561

通讯作者： Sun, Zhihong;Yin, Yulong;Jiang Qingyan;Zhu, Weiyun

作者机构： [Sun, Jiajing; Zhen, Jifu; Wu, Zhaoliang; Rui An; Jing Yang; Wu, Liuting; Sun, Zhihong; Mao Li; Tang, Zhiru; Xu, Qingqing; Zhang, Xiangxin; Cheng Chen; Chen, Jinchao] Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Li, Yunxia] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.;[Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Huang, Feiru] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Sun, Zhihong] S;[Yin, Yulong] C;[Jiang Qingyan] H;[Zhu, Weiyun] N;Southwest Univ, Coll Anim Sci & Technol, Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： Amino acids;Ascorbic acid;Bacteria;Cells;Chemical activation;Cytology;Dichloroacetic acid;Mammals;Metabolism;Nutrients;Phosphorylation;Physiology;Sodium compounds;-glutamate;Dichloroacetate;Glucose consumption;Nutrient consumption;Oxidative metabolism;Pyruvate dehydrogenase kinase;Pyruvates;Small interfering rna (siRNA);Glucose;amino acid;dichloroacetic acid;glucose;pyruvate dehydrogenase complex;pyruvic acid derivative;analysis;animal;bacterium;cell line;dietary supplement;drug effects;intestine;metabolism;microbiology;pig;Amino Acids;Animals;Bacteria;Cell Line;Dichloroacetic Acid;Dietary Supplements;Glucose;Intestines;Pyruvate Dehydrogenase Complex;Pyruvates;Swine

摘要： The extensive metabolism of amino acids (AA) as fuel is an important reason for the low use efficiency of protein in pigs. In this study, we investigated whether regulation of the pyruvate dehydrogenase kinase (PDK)/pyruvate dehydrogenase alpha 1 (PDHA1) pathway affected AA consumption by porcine intestinal epithelial (IPEC-J2) cells and intestinal bacteria in pigs. The effects of knockdown of PDHA1 and PDK1 with small interfering RNA (siRNA) on nutrient consumption by IPEC-J2 cells were evaluated. IPEC-J2 cells were then cultured with sodium dichloroacetate (DCA) to quantify AA and glucose consumption and nutrient oxidative metabolism. The results showed that knockdown of PDHA1 using siRNA decreased glucose consumption but increased total AA (TAA) and glutamate (Glu) consumption by IPEC-J2 cells (P < 0.05). Opposite effects were observed using siRNA targeting PDK1 (P < 0.05). Additionally, culturing IPEC-J2 cells in the presence of 5 mM DCA markedly increased the phosphorylation of PDHA1 and PDH phosphatase 1, but inhibited PDK1 phosphorylation (P < 0.05). DCA treatment also reduced TAA and Glu consumption and increased glucose depletion (P < 0.05). These results indicated that PDH was the regulatory target for shifting from AA metabolism to glucose metabolism and that culturing cells with DCA decreased the consumption of AAs by increasing the depletion of glucose through PDH activation. © 2018 American Chemical Society.

语种： 英文

作者： Dong, G. F.;Yang, Y. O.*;Yao, F.;Chen, L.;Yue, D. D.;...

期刊： Aquaculture Nutrition,2017年23(1):101-110 ISSN：1353-5773

通讯作者： Yang, Y. O.

作者机构： [Liu, L. H.; Huang, F.; Yu, D. H.; Dong, G. F.; Liu, J.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Liu, L. H.; Huang, F.; Yu, D. H.; Dong, G. F.; Liu, J.] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Hubei, Peoples R China.;[Yao, F.; Yue, D. D.; Chen, L.; Yang, Y. O.] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.

通讯机构： [Yang, Y. O.] A;Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.

关键词： amino acid composition;fatty acid profile;feeding rate restriction;growth performance;Pelteobagrus fulvidraco

摘要： Fish growth is an important index in aquaculture practice. However, the effect of growth under restricted feeding on quality of fish grown to the same size remains poorly unknown. In this study, yellow catfish were reared by feeding them at three different feeding rates such as low (FR-L group), medium (FR-M group) and high (apparent satiation) ration levels for 82, 58 and 34 days to reach the same size, respectively. After this period, the growth performance, whole-body amino acid composition and fatty acid profile of the fish were evaluated. Feeding restriction led to significant decreases in specific growth rate and feed conversion efficiency. Significant increases in dry matter, protein and energy contents were observed in fish under both restricted feeding treatments. FR-L-treated fish had significantly lower whole-body essential amino acid contents, and significantly higher Ala and non-essential amino acid contents compared to fish fed to satiation. Fish in restricted feeding treatments had significantly higher n-3 PUFA and n-3/n-6 ratio compared to the fish fed to satiation. The present results indicate that from a quality perspective, there are certain advantages in improvement of whole-body composition and enrichment of n-3 PUFA associated with the restricted feeding of yellow catfish at slow growth rate.

语种： 英文

作者： 邹琦;余登航;黄峰;杨严鸥;魏帮鸿;...

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,中国,武汉 430023;淡水水产健康养殖湖北省协同创新中心,中国,武汉 430070;安徽农业大学动物科技学院,中国,合肥 230000

会议名称： 第十届世界华人鱼虾营养学术研讨会

会议时间： 2015-10-22

会议地点： 武汉

会议论文集名称： 第十届世界华人鱼虾营养学术研讨会论文集

关键词： 草鱼;生长性能;脂肪细胞因子;基因表达

摘要： 　　为进一步确定共轭亚油酸(CLA)对草鱼肝脏的降脂功效，本研究采用65d、无鱼粉和鱼油配方的生长实验、显微和超微病理观察确定CLA显著降低其肝脏脂肪含量且不影响其生长和健康的添加量(CLAOPT)，并探讨CLA在草鱼肝脏中发挥降脂功效的潜在机理.共配制7种等氮等脂饲料，其中CLA添加量分别为：0％(对照组)、0.6％、1.2％、1.8％、2.4％、3％、3.6％，分别补充椰子油至3.6％的总外源脂肪水平.

语种： 中文

作者： 董桂芳;余登航;陈路;黄峰;朱晓鸣;...

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,中国,武汉 430023;淡水水产健康养殖湖北省协同创新中心,中国,武汉430070;安徽农业大学动物科技学院,中国,合肥 230000;中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,中国,武汉,430072

会议名称： 第十届世界华人鱼虾营养学术研讨会

会议时间： 2015-10-22

会议地点： 武汉

会议论文集名称： 第十届世界华人鱼虾营养学术研讨会论文集

关键词： 氧化鱼油;脂肪代谢;瓦氏黄颡鱼

摘要： 　　采用60d的生长实验研究氧化鱼油对瓦氏黄颡鱼(Pelteobaggrus vachelli)脂肪代谢的干扰作用,并探讨PPARα 和SREBP1是否介导其干扰作用,随后通过60d恢复实验研究其恢复效果.共配制4种等氮等脂实验饲料,其中对照组不添加氧化鱼油,其他3个处理组氧化鱼油添加量分别为4(LOO),6(MOO),8％(HOO),分别补充8、4、2、0％的新鲜鱼油至8％的总外源脂肪添加量.实验结果表明,在氧化鱼油饲喂阶段：各氧化鱼油组的终末体重(FBW)和特定生长率(SGR)均显著低于对照组(P＜0.05).MOO组和HOO组血浆中胆固醇(TC)及肝脏中甘油三酯(TG)含量均显著低于对照组(P＜0.05).各氧化鱼油组肝中PPARα mRNA表达量均显著低于对照组(P＜0.05),MOO和HOO组肝中SREBP1 mRNA表达水平均显著高于对照组(P＜0.05).经过60d恢复,各氧化鱼油组的FBW、血浆和肝中TG和TC含量均与对照组无显著性差异(P＜0.05).MOO和HOO组SGR均显著高于对照组(P＜0.05).各氧化鱼油组肝中PPARα mRNA表达量均显著高于对照组(P＜0.05),LOO组和HOO组肝脏中PPARα和SREBP1 mRNA表达量均显著高于对照组(P＜0.05).综上结果表明,过氧化值为12.42-35.35 meq/kg的氧化鱼油可显著干扰其组织脂肪代谢,该干扰效应可能与氧化鱼油作为配体激活PPARα和SREBP1 mRNA表达有关；经过60d的恢复,各处理组实验鱼生长性能和组织脂肪含量均已恢复至正常水平,但PPARα 和SREBP1 mRNA是否被补偿激活还有待进一步的确定.

语种： 中文

作者： Dong, G. F.;Yang, Y. O.*;Yao, F.;Chen, L.;Bu, F. Y.;...

期刊： Journal of Applied Ichthyology,2015年31(2):349-354 ISSN：0175-8659

通讯作者： Yang, Y. O.

作者机构： [Yao, F.; Chen, L.; Dong, G. F.; Yang, Y. O.; Li, P. C.; Bu, F. Y.] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.;[Huang, F.; Yu, D. H.; Dong, G. F.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.;[Huang, F.; Yu, D. H.; Dong, G. F.] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Hubei, Peoples R China.

通讯机构： [Yang, Y. O.] A;Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.

关键词： Oreochromis niloticus;Tilapia

摘要： A 30-day growth trial was conducted to investigate the individual variations in feeding rate, growth rate, spontaneous activity, and their possible interrelationships in hybrid tilapia (Oreochromis niloticus × O. aureus) reared individually at feeding frequencies of one and two meals daily. Results show that the feeding rate in energy (FRe), specific growth rate in energy (SGRe), and food conversion efficiency (FCE) of fish fed twice a day were significantly higher than that of fish fed only once. However, no significant differences in distance or in time spent swimming were observed between the groups. SGRe was positively correlated with FRe in fish fed only once daily, and SGRe was positively correlated with food conversion efficiency in energy (FCEe) between the two groups. SGRe was only inversely correlated with the distance that fish swam when fed once daily. The results suggest that the individual growth differences of hybrid tilapia could be attributed mainly to variations in FRe, and that the energy costs of spontaneous activity play an important role in the differences at feeding frequency of one meal a day. However, at feeding frequencies of two meals a day, individual growth differences in fish may be caused by variations in FCEe, and energy costs of spontaneous activity have only marginal effects on the differences. © 2014 Blackwell Verlag GmbH.

语种： 英文

作者： 王进;刘恒见;黄峰;韩金林

期刊： 淡水渔业,2014年44(4):105-108 ISSN：1000-6907

作者机构： [刘恒见; 王进; 黄峰; 韩金林] 武汉轻工大学

关键词： 铁源;吉富罗非鱼(Oreochromis niloticus);生长;表观消化率

摘要： 为观察不同铁源对吉富罗非鱼（ Oreochromis niloticus）生长及表观消化率的影响，试验以初重6.79 g左右的吉富罗非鱼为饲养对象，采用酪蛋白、糊精为主要原料制成半精制日粮，分别添加铁含量相等（100 mg/kg饲料）的硫酸亚铁、氯化铁、氯化亚铁、柠檬酸亚铁、卟啉铁，饲喂吉富罗非鱼70 d。结果显示，在铁含量相等（100 mg/kg饲料）的半精制日粮中添加氯化亚铁，吉富罗非鱼的增重率显著（ P＜0.05）高于其它四种铁源。以增重率为指标，吉富罗非鱼日粮中适宜以氯化亚铁作为添加铁源。

语种： 中文

作者： 聂伟;刘立鹤;刘军;黄峰;王哲;...

期刊： 江西水产科技,2014年(4):43-48 ISSN：1006-3188

作者机构： 武汉轻工大学动物科学与营养工程学院;[王哲; 周哲] 标优美生态工程股份有限公司;[刘军; 聂伟; 黄峰; 刘立鹤] 武汉轻工大学

关键词： 生物絮团;絮凝机理;影响因素;应用问题

摘要： 生物絮团是养殖水体中以好氧微生物为主体的有机体和无机物，经生物絮凝形成的团聚物，由细菌、浮游动植物、有机碎屑和一些无机物质相互絮凝组成。通过对生物絮团研究的发展过程、絮团的结构特征、絮凝机理、影响絮团的形成因素以及生物絮团技术在水产养殖应用中存在的问题进行了综述，为生物絮团技术在水产领域中的进一步研究和应用具有一定的指导意义。

语种： 中文

作者： Li, Weidong;Wang, Chong;Huang, Feng;Li, Ming*;Nilsen, Frank;...

期刊： PARASITE,2014年21:46 ISSN：1252-607X

通讯作者： Li, Ming

作者机构： [Li, Ming; Huang, Feng; Li, Weidong; Xu, Jianlong; Liu, Huiyu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wang, Chong] Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China.;[Wang, Chong] Chinese Acad Sci, Wuhan 430079, Peoples R China.;[Li, Ming; Nilsen, Frank] Univ Bergen, Dept Biol, Sea Lice Res Ctr, N-5020 Bergen, Norway.;[Li, Ming] Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan 430070, Peoples R China.

通讯机构： [Li, Ming] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

关键词： animal;China;classification;isolation and purification;parasitology;Ranidae;rectum;scanning electron microscopy;species difference;stramenopile;symbiosis;ultrastructure;Animals;China;Microscopy, Electron, Scanning;Ranidae;Rectum;Species Specificity;Stramenopiles;Symbiosis

摘要： A redescription of Protoopalina pingi Nie, 1935 is presented in this paper to complete Nie's description at both light and scanning electron microscope levels. These organisms were collected from the recta of the frogs Hylarana guentheri Boulenger, 1882 and Pelophylax nigromaculatus Hallowell, 1861 from Jialing River, Sichuan Province and Honghu Lake, Hubei Province, respectively, in China. This is the first record of its occurrence in H. guentheri and P. nigromaculatus. The body of P. pingi is elongated and somewhat spindle-like in shape, slightly narrowed and bluntly rounded at the anterior extremity, while the posterior end is tapering or sharply pointed. The body surface is thickly flagellated, with the caudal tip being barren. The falx, located at the margin of the anterior end, is composed of a narrow band of kinetosomes. Four round or oval-shaped nuclei, usually arranged in a straight line, are situated in the middle region of the body. Comparisons are made between P. pingi and its congeners. © 2014 W. Li et al., published by EDP Sciences.

语种： 英文

作者： Dong, Gui-Fang;Zou, Qi;Wang, Huan;Huang, Feng;Liu, Xiao-Chun;...

期刊： Aquaculture,2014年432:181-191 ISSN：0044-8486

通讯作者： Yang, Yan-ou

作者机构： [Wang, Huan; Huang, Feng; Liu, Xiao-Chun; Dong, Gui-Fang; Yang, Chao-Yu; Zou, Qi] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Yang, Yan-ou; Chen, Lu] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.;[Huang, Feng; Dong, Gui-Fang] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China.

通讯机构： [Yang, Yan-ou] A;Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China.

关键词： Conjugated linoleic acid;Ctenopharyngodon idella;Fatty liver;Gene expression;Lipid deposition

摘要： Conjugated linoleic acid (CLA) has been shown to decrease body fat and increase lean tissue in mammals. However, limited data is available about the effect of CIA on the lipid content in fish tissue, and the mechanisms underlying the beneficial effects of CLA in fish are unknown. We hypothesized that dietary CIA may induce lipid-lowering effects in grass carp (Ctenopharyngodon idella) tissue, and the fat reduction effect was modulated by the expression of genes involved in the lipid metabolism. A 65-day growth trial was conducted to investigate the effect of CIA on the growth, tissue lipid deposition, and gene expression involved in the lipid metabolism of grass carp. Seven isonitrogenous and isolipidic diets were formulated: 0% CIA (control); 0.5% CIA (CLA0.5); 1% CIA (CLA1); 1.5% CIA (CLA1.5); 2% CIA (CLA2); 2.5% CIA (CLA25); and 3% CIA (CLA3). Results showed that only fish fed the CLA3 diet exhibited a significant reduction in feeding rate and specific growth rate than those of fish fed the control diet (P < 0.05). Significant decreases in the lipid content in the liver, intraperitoneal fat, and muscle were observed in fish fed with 2.5% to 3% CIA, 1.5% to 3% CIA, and 2% to 3% CIA diets, respectively (P<0.05), compared to those fed with the control diet. Dose- and tissue-dependent changes were found in the mRNA expressions of fatty acid synthetase (FAS), acetyl-CoA carboxylase (ACC), lipoprotein lipase (LPL), hormone-sensitive lipase (HSL), peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma (PPAR gamma), and sterol regulatory element binding protein-1c (SREBP-1c). The mRNA expressions in most of the genes examined in the liver, foregut, intraperitoneal fat and muscle were highly sensitive to dietary CIA. Our results suggested that a dose-dependent effect on the reduction of fish growth induced by CIA supplementation should be carefully considered in intensive aquaculture, although lipid reduction is nutritionally important for fatty liver control in grass carp. Furthermore, our results raise the possibility that the lipid-lowering effects of dietary CIA were modulated by the gene expressions in lipogenesis (such as FAS and ACC), lipoprotein transport (such as LPL), and lipolysis (such as HSL) primarily in the liver, foregut, intraperitoneal fat, and muscle. The activation of transcription factors (such as PPAR alpha, PPAR gamma, and SREBP-1c) may also be responsible for the lipid-lowering effects of dietary CIA (C) 2014 Elsevier B.V. All rights reserved.

语种： 英文

作者： 刘立鹤;张恒;聂伟;陈进;张志勇;...

期刊： 武汉轻工大学学报,2014年33(1):34-39 ISSN：2095-7386

作者机构： 武汉轻工大学动物科学与营养工程学院;湖北省淡水水产健康养殖协同创新中心;[范伟] 武汉爱维信生物科技有限公司;[刘军; 聂伟; 张恒; 张志勇; 陈进; 黄峰; 刘立鹤] 武汉轻工大学

关键词： 草鱼;酵母培养物;生长性能;肝胰腺;组织切片

摘要： 试验在两种不同营养水平的饲料配方中分别添加0 mg/kg,50 mg/kg,100 mg/kg的酵母培养物益宁易SCP,制成6种试验饲料饲喂均重20.39±2.19g草鱼6周,评估了饲料中不同益宁易添加量对草鱼生长性能和肝、肠功能的影响.结果表明:在普通饲料中添加50 mg/kg的益宁易SCP可以促进草鱼的生长,保护草鱼的肝脏不受损害,并能够显著促进草鱼肠道的发育,增加其绒毛高度;而在降低营养标准的低价饲料中,添加量要需增加到100 mg/kg,效果才会更明显.

语种： 中文

作者： 刘成;黄峰;邱银生

期刊： 渔业致富指南,2014年(4):57-59 ISSN：1008-2840

作者机构： 武汉轻工大学动物科学与营养工程学院;[刘威; 邱银生; 黄峰] 武汉轻工大学

关键词： 苯甲酸;寄生虫;预混剂;水产养殖;养殖生产;阿维菌素;大西洋鲑鱼;推荐剂量;皮肤;细胞增生

摘要： <正>在水产养殖生产中,经常出现一些寄生虫如鲺的病害侵袭,这些寄生虫常以其口器及背甲腹面的倒刺刺伤鱼的头部、背部以及肛周,撕裂宿主（鱼类）皮肤,摄食鱼的皮肤、粘液和血液,使宿主十分不安,病鱼出现狂游并跳离水面,若寄生在鳃部可造成鳃组织的炎性水肿和细胞增生,外观上出现鳃丝末端肿大发白。寄生虫对鱼体的这种机械性刺激、损

语种： 中文

作者： 李明;李伟东;戈贤平;汪建国;龚小宁;...

作者机构： [李明; 李伟东; 戈贤平; 汪建国; 龚小宁; 黄峰; 刘辉宇] 武汉工业学院湖北省动物营养与饲料科学重点实验室;[李明; 李伟东; 戈贤平; 汪建国; 龚小宁; 黄峰; 刘辉宇] 中国水产科学研究院淡水渔业研究中心;[李明; 李伟东; 戈贤平; 汪建国; 龚小宁; 黄峰; 刘辉宇] 中国科学院水生生物研究所

会议名称： 中国水产学会鱼病专业委员会2013年学术研讨会

会议时间： 2013-11-05

会议地点： 中国海南海口

会议论文集名称： 中国水产学会鱼病专业委员会2013年学术研讨会论文摘要汇编

关键词： 珠蚌累枝虫;黄颡鱼鱼苗;缘毛亚纲;皮肤损伤

摘要： 累枝虫（Epistylis sp.）隶属于缘毛亚纲,固着目,累枝虫科;是一类广泛附生于多种水生植物、无脊椎动物和脊椎动物的纤毛虫。在一定情况下,可以严重危害养殖的鱼苗、鱼种、鳖及各种虾、蟹的卵、幼体和成体。其中尤以对虾、蟹的幼体和鱼苗的危害最大,可引起大批死亡,残存的商品价值也大大降低。珠蚌累枝虫（Epistylis unioi）是龚循矩（1986）在圆顶珠蚌的鳃叶上发现并命名的。本文以寄生在黄颡鱼鱼苗的珠蚌累枝虫为主要研究对象,利用蛋白银染色法和扫描电镜技术,对这一虫种进行了再描述。珠蚌累枝虫在黄颡鱼苗体表的发现是其宿主的一个新纪录。结果表明:黄颡鱼鱼苗为大量的珠蚌累枝虫感染,鱼体表现为生命力减弱,游动缓慢,浮头、呼吸急促;置于解剖镜下观察发现大量的虫体存在于鱼体体表、鳍、须和鳃上。虫体细长,略呈花瓶状,活体状态下虫体完全伸展时体长56-88（68）μm,体宽22-38（30）μm,口围唇的直径27-45（35）μm。蛋白银染色状态下,体长34-53（41）μm,体宽16-26（22）μm。虫体表面可见多条横向排列的条纹,在口围与横纤毛带间有37-45（41）条,横纤毛带与帚胚间有26-33（29）条,相邻条纹间距约为0.5μm。横向条纹上可见许多排列稀疏的气孔。单毛基列和复毛基列均起自于细胞顶端口围唇的内侧,两者相伴而行,进入口区之前围绕口缘1.5圈。P1-3分别由三条毛基索组成,其中P1、P2远长于P3;P1的下端一直延伸至胞口处,而P2和P3末端交汇,位于P1的下端点之上。伸缩泡一个,位于虫体顶端口围盘稍下处。大核呈马蹄形,横位,位于虫体的前部。群体较小,呈不对称二叉式分支。此外,我们还发现了虫体的游泳体,在横纤毛带上生出密集的纤毛。扫描电镜显示,在虫体的体柄与寄主皮肤接触的部位,可见明显损伤。

语种： 中文