摘要：
Polysaccharides are considered to be promising candidates for non-viral gene delivery because of their molecular diversity, which can be modified to fine-tune their physicochemical properties. In this work, transcriptional activator protein (TAT) functionalized PEI grafted polysaccharide polymer (PRBP) was prepared by using rice bran polysaccharide as the starting material, and characterized by various methods. The potential of TAT functionalized PRBP (PRBP-TAT) as gene vector was studied in vitro, including DNA loading capacity, DNA protection ability and biocompatibility. The cell uptake and transfection efficiency of the PRBP-TAT/pDNA polyplexes were studied. The results showed that PRBP-TAT could completely condense DNA at N/P 2. The PRBP-TAT/pDNA polyplexes could protect DNA from degrading by DNase and were efficiently internalized by cells. Biocompatibility result showed that PRBP-TAT had no significant cytotoxicity and effect on cell proliferation. At low N/P ratios of 1-3.5, PRBP-TAT showed higher transfection efficiency than PEI30k and PEI30k-grafted rice bran polysaccharide. PRBP-TAT and PEI showed the highest transfection efficiency of 42.8% and 28.1% when pDNA is 2 microg and N/P ratio is 1.5, respectively, while PRBP showed the highest transfection efficiency of 37.3% at N/P 2.5. These results indicate that PTA is a promising candidate vector for safe and efficient gene delivery.
作者机构：
[Liu, Jianfang; Wei, Lei; Duan, Haitao; Jin, Yongliang; Liu, Lian; Sun, Xianming; Jia, Dan; Chen, Song; Li, Jian] Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.;[Liu, Jianfang] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Sun, Xianming] Wuhan Inst Technol, Sch Mat Sci & Engn, Wuhan 430073, Peoples R China.
通讯机构：
[Li, Jian] W;Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.
关键词：
motor oil;oil condition evaluation;on-board diagnostic system
摘要：
The condition of the motor oil in civilian cars is difficult to monitor; hence, we propose a method to evaluate the degree of degradation of motor oil using an on-board diagnostic (OBD) system. Three civilian cars and four motor oils (containing mineral oils and synthetic oils) were subjected to five groups of road tests under urban traffic and high-way conditions. The operation information, oil service time, mileage, engine operation time, idle time of the engine, and number of start-ups of the engine were obtained using the proposed OBD system. Physiochemical properties and changes in the components of motor oils during road tests were analyzed in laboratory. The theoretical model of the comprehensive indicators of driving parameters and oil properties were established. The proposed method was successfully applied to different cars, motor oils, and operating conditions in road tests. All the theoretical models had high accuracy and precision. Herein, we provide a method to monitor the oil condition with real-time driving parameters and provide a reference for end users to change their motor oil reasonably.
摘要：
Metal complexes, as a type of potential non-virus gene carriers, have gained much attention due to their properties of high charge density and unique three-dimensional structure. This study investigated the potential of polyethyleneimine (PEI)-modified rice bran polysaccharide-Fe(III) complex (PEI-PI) as a safe gene delivery system and explored the effect of Fe(III) on the efficiency of gene transfection mediated by PEI modified rice bran polysaccharide (PEI-P) and PEI-PI. Gel retardation assay was used to study the DNA binding and protection capability, MTT assay was performed to evaluate the biocompatibility, and PEI-PI complex-mediated EGFP gene transfection was studied in vitro. Results showed the PEI-PI could induce DNA condensation and protect DNA from degradation by DNase I at a low weight ratio (vector/DNA) of 2. At the same weight ratio, PEI exhibited the strongest DNA binding capability but PEI-PI exhibited the highest gene transfection efficiency among all carrier systems. Compared with the PEI-P + Fe(III) system, PEI-PI not only had a more significant capability to condense DNA but also presented higher gene transfection efficiency. Moreover, PEI-PI exhibited no obvious cytotoxicity to cells. This work provides a strategy for the design and development of gene vectors based on PI complexes. (C) 2019 Elsevier B.V. All rights reserved.
摘要：
Polysaccharides are capable of being modified by polycations to adjust their physical and chemical properties, which accordingly are considered as potential candidate materials for safe and efficient gene delivery. Here, we extracted and purified polysaccharides from rice bran, and their physicochemical properties were determined by various methods. Polyethyleneimine (PEI) modified rice bran polysaccharide (PRBP) was prepared by grafting RBP with low molecular weight PEI and the preparation was determined by FTIR. The potential of PRBP as a gene vector was systematically evaluated in vitro. The results show that PRBP can compact DNA and form PRBP/DNA polylexes with a particle size of 50-100 nm. The PRBP/DNA polylexes can protect DNA degradation from DNase I efficiently. Compared with PEI, higher transfection efficiency was achieved by the PRBP. At weight ratio of 3, the highest efficiency of gene transfection mediated by PRBP-2000 was obtained, which was 37.5% and significantly higher than PEI and commercial reagents (calcium phosphate cell transfection kit) and was closed to lipo6000. Furthermore, according to MTT results, the cytotoxicity of PRBP is much lower than that of PEI, especially for PEI2000. We hope these results will provide new strategy for rice bran polysaccharides development and application as biomaterials. (C) 2019 Elsevier B.V. All rights reserved.
摘要：
The effect on the intracellular reactive oxygen species (ROS) generation, and the antioxidant and cytotoxicity properties of rice bran polysaccharides (RBP) and RBP-metal complexes RBP-Fe(III), RBP-Cu, RBP-Zn and RBP-Ca, were evaluated using atomic absorption spectroscopy (AAS), scavenging activity assays, cell viability assay and fluorescence microscopy. The RBP-metal complexes were prepared using the hydrothermal method. The RBP-Fe(III) complexes were found to be potent scavengers for superoxide (O2-center dot) free radicals. The RBP alone and RBP-Ca complex showed high scavenging activity for 2,2-diphenyl-l-picrylhydrazyl (DPPH center dot) free radicals. In addition, the RBP-Fe(III) complex also showed good biocompatibility and lowered the intracellular ROS levels, while RBP alone, RBP-Zn and RBP-Ca complexes were observed to increase the intracellular ROS level. Our findings suggest that among the tested RBP-metal complexes, RBP-Fe(III) complex is a strong candidate as an antioxidant therapeutic. (C) 2018 Elsevier B.V. All rights reserved.
摘要：
The effects of n3 polyunsaturated fatty acids (PUFA) on cardiovascular disease are controversial. We currently explored the effects of various ratios of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on high-fat-induced atherosclerosis. In model apoE(-/-) mice, high-fat diets (HFD) were partially replaced with fish and algal oils (DHA/EPA 2:1, 1:1 and 1:2) and/or plant oils enriched in linoleic and alpha-linolenic acids with an n6/n3 ratio of 4:1. PUFA supplementation significantly reduced the atherosclerotic plaque area, serum lipid profile, inflammatory response, aortic ROS production, proinflammatory factors and scavenger receptor expression as compared to those in the HFD group. However, plant oils did not have a significant effect on the following: serum HDL-C level; aortic ABCA1, ABCG1 and LAL mRNA expression; and CD36 and LOX-1 protein expression. Compared to the plant-oil-treated group, the DHA/EPA 1:1 group had a smaller atherosclerotic plaque area, higher serum HDL-C levels and lesser CD36 and MSR-1 mRNA expression; the DHA/EPA 2:1 group had lower serum TC, LDL-C and TNF-a levels and lower aortic ROS levels. Our study suggested that n3 PUFA from animals had more potent atheroprotective effects than that from plants. Supplementation involving higher DHA/EPA ratios and an n6/n3 ratio of 4:1 was beneficial for reducing serum "bad cholesterol" and a 1:1 DHA/EPA ratio with an n6/n3 ratio of 4:1 was beneficial for improving serum "good cholesterol" and inhibiting ox-LDL uptake. Our results suggest that achieving an n6/n3 ratio of 4:1 in the diet is also important in addition to having an optimal DHA/EPA ratio. (C) 2016 Elsevier Inc. All rights reserved.
作者：
Liang Liu;Xiao Ming Song;Jin Tao Guan;Zhi Yong Zhang;Ben Mei Wei
期刊：
Asian Journal of Chemistry,2015年27(6):2093-2095 ISSN：0970-7077
通讯作者：
Guan, J.T.
作者机构：
[Liu L.] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China;[Wei B.M.; Guan J.T.; Zhang Z.Y.; Song X.M.] School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Guan, J.T.] S;School of Chemical and Environmental Engineering, China
关键词：
Dppc+-PF6-;Heck coupling reaction;Recyclable
摘要：
An efficient PdCl2-Dppc+-PF6--[bmim][PF6] catalytic system has been developed for the Heck coupling reaction of various aryl iodides with olefins. The catalytic system allows the coupling reaction to proceed smoothly in excellent yields and can be recycled 5 times with minimal loss of activity.
摘要：
Article Crystal structure of bis(3,5-dichloro-N-2-(dimethylamino)ethylsalicylaldiminato- κ3N,N',O)manganese(II) monohydrate, Mn(C11H13Cl2N2O)2 · H2O was published on June 1, 2012 in the journal Zeitschrift für Kristallographie - New Crystal Structures (volume 227, issue 2).
