作者机构：
[Liu, Jianfang; Wei, Lei; Duan, Haitao; Jin, Yongliang; Liu, Lian; Sun, Xianming; Jia, Dan; Chen, Song; Li, Jian] Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.;[Liu, Jianfang] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Sun, Xianming] Wuhan Inst Technol, Sch Mat Sci & Engn, Wuhan 430073, Peoples R China.
通讯机构：
[Li, Jian] W;Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Peoples R China.
关键词：
motor oil;oil condition evaluation;on-board diagnostic system
摘要：
The condition of the motor oil in civilian cars is difficult to monitor; hence, we propose a method to evaluate the degree of degradation of motor oil using an on-board diagnostic (OBD) system. Three civilian cars and four motor oils (containing mineral oils and synthetic oils) were subjected to five groups of road tests under urban traffic and high-way conditions. The operation information, oil service time, mileage, engine operation time, idle time of the engine, and number of start-ups of the engine were obtained using the proposed OBD system. Physiochemical properties and changes in the components of motor oils during road tests were analyzed in laboratory. The theoretical model of the comprehensive indicators of driving parameters and oil properties were established. The proposed method was successfully applied to different cars, motor oils, and operating conditions in road tests. All the theoretical models had high accuracy and precision. Herein, we provide a method to monitor the oil condition with real-time driving parameters and provide a reference for end users to change their motor oil reasonably.
摘要：
The original version of this article unfortunately contained a mistake in the Fig. S1 of supplementary material. It is corrected with this erratum.
期刊：
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART J-JOURNAL OF ENGINEERING TRIBOLOGY,2019年233(2):281-288 ISSN：1350-6501
通讯作者：
Li, Jian
作者机构：
[Liu, Jianfang; Wei, Lei; Duan, Haitao; Jin, Yongliang; Jia, Dan; Chen, Song; Qian, Xuzheng; Li, Jian] Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Hubei, Peoples R China.;[Liu, Jianfang] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.
通讯机构：
[Li, Jian] W;Wuhan Res Inst Mat Protect, State Key Lab Special Surface Protect Mat & Appli, Wuhan 430030, Hubei, Peoples R China.
关键词：
Motor oil;oil drain interval;driving parameters;grey relational analysis;on-board diagnostic
摘要：
Although the reason is not clear, mileage is commonly recommended as an indicator to evaluate the oil drain interval of a car. This paper tries to investigate whether the mileage is the most suitable indicator to evaluate the oil drain interval based on scientific experiments and data analysis method. Three civilian cars and motor oils (contained mineral oil, semisynthetic oil, and synthetic oil) were subjected to road tests under urban traffic condition. The real-time driving parameters comprising speed, mileage, idle time, engine operation time, and number of starts were obtained by on-board diagnostic system. Synchronous oil samples were collected about every 30 days. The physicochemical properties and component changes of experimental oils were analyzed in laboratory. The effects of driving parameters on oil properties were studied with the improved grey relational analysis method. The results show that the influencing factors of driving parameters on motor oil degradation were different in different experimental cars. The influence of mileage on oil degradation was not obvious. The idle time of engine, engine operation time, and number of starts were the three most significantly influential factors on oil deterioration after comprehensive analysis of the three experimental cars.
摘要：
Acinetobacter sp. YT-02, a Gram-negative bacterium isolated from the activated sludge from a sodium N-cyclohexylsulfamate production plant, has the ability to degrade cyclohexylamine. It was classified as a member of Acinetobacter sp., a Gram-negative bacterium, sharing a 16S rRNA gene sequence identity of 99% with Acinetobacter guangdongensis strain 1NM-4. It could degrade 10 mmol/L cyclohexylamine within 22 h. Based on the identified metabolite, the metabolic pathway of cyclohexylamine could be postulated as it was degraded via cyclohexanone. Draft genome sequence of this strain (2,993, 647 bp of chromosome length) is presented here. We further identified the genes encoding the enzymes involved in cyclohexylamine oxidation to cyclohexanone and the subsequent downstream metabolic pathway of cyclohexanone oxidation. Strain YT-02 has the potentiality to be applied in the treatment of the pollutant cyclohexylamine, and it could also be treated as a research material to study the degradation mechanism of cyclohexylamine.
摘要：
A consortium comprised of an engineered Escherichia
coli DH5α and a natural pentachlorophenol (PCP) degrader, Sphingobium
chlorophenolicum ATCC 39723, was assembled for degradation of hexachlorobenzene (HCB), a persistent organic pollutant. The engineered E. coli strain, harbouring a gene cassette (camA
+
camB
+
camC) that encodes the F87W/Y96F/L244A/V247L mutant of cytochrome P-450cam (CYP101), oxidised HCB to PCP. The resulting PCP was then further completely degraded by ATCC 39723. The results showed that almost 40 % of 4 μM HCB was degraded by the consortium at a rate of 0.033 nmol/mg (dry weight)/h over 24 h, accompanied by transient accumulation and immediate consumption of the intermediate PCP, detected by gas chromatography. In contrast, in the consortium comprised of Pseudomonas
putida PaW340 harbouring camA
+
camB
+
camC and ATCC 39723, PCP accumulated in PaW340 cells but could not be further degraded, which may be due to a permeability barrier of Pseudomonas PaW340 for PCP transportation. The strategy of bacterial co-culture may provide an alternative approach for the bioremediation of HCB contamination.
作者机构：
[Liu, Jianfang; Wang, Ding; Gu, Kali; Zhao, Yuan] Wuhan Res Inst Mat Protect, Wuhan 430030, Hubei, Peoples R China.;[Liu, Jianfang] Wuhan Polytech Univ, Dept Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Jianfang] W;Wuhan Res Inst Mat Protect, Wuhan 430030, Hubei, Peoples R China.
关键词：
Hydraulic Fluid;Lubricant Physical and Chemical Analysis;Lubricant Recycling;Lubrication Performance
摘要：
Used lubricating oil contains more than 90% of good base oil and available additives, providing the potential of energy conservation, environmental protection, and economy, though it loses its useful performance because of contamination by impurities, oxidation, and degradation of additives. Many countries around the world have focused on reclamation and re-refining methods to treat and reuse waste lubricating oils for many years. But the regeneration rate of waste oils is very low in China, less than 20% of the lubricant consumption. Few studies on the performance, especially lubrication properties of recycled lubricating oils, have been considered. In this article, based on differences between used and fresh oils, used oils were reclaimed and replenished with additives (henceforth refortified oils). The physical and chemical properties, including viscosity, total acid number, moisture content, copper corrosion, and water separability from petroleum oil and synthetic fluid as well as the lubrication characteristics of three kinds of refortified hydraulic oils were investigated. The lubrication performance was evaluated using a four-ball tribotester and a Universal Micro-Tribotester (UMT) reciprocating tester. Moreover, the worn surfaces were analyzed using confocal scanning optical microscopy (CSOM), scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. The results showed that refortified oils provided good physical and chemical properties, close to those of fresh oils, and produced better tribological characteristics than used and even fresh oils. There were compositional differences between the films of fresh, used, and refortified oils.
作者机构：
[冀志霞; 陈守文; 马昕; 付军涛; 祁高富] State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;[刘军] College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Chen, S.] S;State Key Laboratory of Agricultural Microbiology, , Wuhan 430070, China
关键词：
地衣芽胞杆菌;聚γ-谷氨酸;ywtD基因;SacB启动子;amy启动子;加强表达
摘要：
聚γ-谷氨酸( γ-PGA)是一种应用前景良好的生物高分子材料.比较了蔗糖诱导的枯草芽胞杆菌果聚糖蔗糖酶基因(SacB)启动子和地衣芽胞杆菌α-淀粉酶基凶启动子对γ-PGA降解酶基因ywtD在地衣芽胞杆菌中加强表达的影响.分别用SacB基因启动子和α-淀粉酶启动子构建了穿梭表达载体pHY300-SYT和pHY300-PYT,通过电转化地衣芽胞杆菌WX-02获得重组子SYT和PYT.酶活测定结果显示SYT和PYT中γ-PGA降解酶基因ywtD得到加强表达,摇瓶发酵结果显示两个重组菌株的γ-PGA相对分子质量都由1 000 000～1 200 000降低为800 000～900 000,PYT的γ-PGA产量较对照菌株PLK提高了33％,由13.50 g L-1提高到17.97 g L-1,而SYT的γ-PGA产量则降低为10.85 g L-1.因此,α-淀粉酶启动子更适合于在地衣芽胞杆菌WX-02菌株中表达γ-PGA降解酶基因,从而获得高产低分子量γ-PGA的工程菌.
