作者机构：
[Fen Teng] Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Longxia Hu; Jiaqing Wang; Yi Dai; Lihong Bao; Yun Yang; Tingting Jiao; Fei Luo; Yali Yang; Zixi Wang; Xiaoping Yang; Shengli Yang] Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Deying Hu; Yilan Liu; Yanhong Han; Xiaoping Ding] Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Wenbin Liu; Hongling Zhang; Qingzhou Cheng] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Deying Hu] D;[Wenbin Liu] C;Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
关键词：
attitude;knowledge;living will;malignant tumor;palliative care
摘要：
Given the suboptimal quality of end-of-life care among patients with cancer in China, promoting living wills is critical in this population. Living wills ensure that individuals can receive the medical care they desire during the terminal phase of an illness, maintain their dignity, and ultimately achieve a good death. However, current awareness and attitudes about living wills among Chinese patients with cancer remain unclear. We administered a questionnaire survey on living wills to patients with malignant tumors to assess their most important needs and increase understanding about living wills.
In this cross-sectional study using convenience sampling, inpatients with malignant tumors in Wuhan completed our questionnaire between July 2020 and June 2021. We collected patients' sociodemographic characteristics and details regarding their knowledge and attitudes about living wills.
Among 213 patients with malignant tumors, 114 (53.52%) had heard of living wills; 125 (58.69%) expressed their willingness to sign the “Five Wishes” living will document after learning about it through the questionnaire. Patients with malignant tumors had a high level of desire for the following living will items: the lives of family and friends return to normal as soon as possible after their death, maintaining personal hygiene and dignity, and remaining pain-free. The knowledge level of patients with malignant tumors was related to their educational level ( p < 0.05) and self-care ability ( p < 0.05).
Patients with malignant tumors have a high need for comfort, cleanliness, and pain relief in the terminal stages. Patients with a higher level of education and those with poorer self-care ability had greater knowledge and acceptance of living wills. Promotion can first be targeted toward more highly educated patients and can then be gradually expanded to other groups.
期刊：
CURRENT MEDICINAL CHEMISTRY,2025年32:1-13 ISSN：0929-8673
作者机构：
[Wenbin Liu] College of Medicine and Health Science, Wuhan Polytechnic
University, Wuhan, 430023, China;[Muhammad Umer] Research Center of Forest
Ecology, Institute for Forest Resources and Environment of Guizhou and Forestry College, Guizhou University,
Guiyang, 550025, China;[Yang Xu] Wuhan Polytechnic University School of Modern Industry for Selenium Science and Engineering Wuhan China;[Jixin Chen; Yi He; Shiya Wei; Zhangqian Wang; Chao Gao] National R&[Jixin Chen; Yi He; Shiya Wei; Zhangqian Wang; Chao Gao] D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center
for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science
and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
Introduction: G-quadruplexes (G4s) are non-classical high-level structures that are formed by DNA/RNA sequences and have been a promising target for developing antitumor drugs. However, it is still a challenge to find a ligand that binds to a particular G4 with selectivity. Telomeric multimeric G4s are more accessible for screening for specific ligands due to their higher-order structure compared with telomeric monomeric G4s.<&wdkj&>Methods : In this study, the natural product berberine was found to exhibit a higher selectivity for telomeric multimeric G4 in comparison with other G4s. The mechanism of interaction between telomeric G4s and berberine was further investigated by fluorescence spectra measurements, job plot analysis, and UV titrations. We found that there are three binding sites for berberine on telomeric dimeric G-quadruplex Tel45, which are located at the 5' and 3' terminal G-quartet surfaces and the pocket between the two quadruplex units of Tel45. It was worth noting that the berberine preferred to interact within the interfacial cavity between two G4 units.<&wdkj&>Results : Moreover, via dynamic light scattering (DLS) and native polyacrylamide gel electrophoresis (Native-PAGE) assays, it was found that the particle size of the telomeric multimeric G4s conformation was significantly increased by the addition of berberine. In contrast, the particle sizes of Tel21 did not change significantly after the addition of berberine. An immunofluorescence assay indicated that berberine induced the formation of endogenous telomeric G4 structures along with the related telomeric DNA damage response.<&wdkj&>Conclusion: This study provides a hypothetical basis for the development of natural products targeting telomeric G4 as antitumor drugs.
作者机构：
[Cheng, Qingzhou; Cheng, Chu; Zhou, Wei; Chen, Yulong; Liu, Wenbin] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.;[Zhang, Zhiling; Ye, Jingsi] Huaren Technol Co Ltd, Wuhu, Peoples R China.;[Xiao, Pengfeng] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing, Peoples R China.
通讯机构：
[Cheng, C ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.
摘要：
Eliminating errors in next-generation sequencing has proven to be challenging. Here we present a novel strategy for DNA sequencing, called correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry. This sequencing method involves introducing a mixture of natural nucleotide X, labeled unblocked nucleotide X ', 3 ' blocked nucleotide Y*, and labeled 3 ' blocked nucleotide Y* into each reaction cycle. By cyclically interrogating a template twice with different nucleotide combinations, two sets of base-encoding are sequentially obtained, enabling accurate deduction of base sequence. We demonstrate the remarkable efficacy of this approach in detecting and correcting sequencing errors, achieving a theoretical error rate of 0.0005%, which is twice as accurate as Sanger sequencing. Furthermore, we show the capability to detect known mutation sites using information from only a single sequencing run. The correctable two-color fluorogenic DNA decoding sequencing approach should enable accurate identification of extremely rare genomic variations in diverse applications in biology and medicine. A correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry.
作者机构：
[刘亚军; 何文; 刘文斌] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, 430023, China;[张倩] Union Hospital, Tongji Medical College, HUST, Wuhan, 430023, China;[陈灵秀] Tongji Hospital, Tongji Medical College, HUST, Wuhan, 430023, China;[吴婧] The Central Hospital of Wuhan, Wuhan, 430023, China;[王丽梅] College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
Background: Evidence has suggested that sensation and socio-psychological factors may be associated with cognitive impairment separately in older adults. However, the association between those risk factors and cognitive impairment is still unknown.Objective: To investigate the association between sensation, perception, negative sociopsychological factors, and cognitive impairment in institutionalized older adults.Methods: From two public aged care facilities, 215 participants were investigated. The Minimental State Examination was applied to assess cognitive function. The sensory function was bifurcated into auditory and somatosensory realms which were evaluated using pure tone audiometry and Nottingham Sensory Assessment, respectively. Albert's test, left and right resolution, and visuospatial distribution were used to evaluate perception. Depression and social isolation were selected as negative socio-psychological factors and were evaluated by the Geriatric Depression Scale and the Lubben Social Network Scale. The multivariate analysis was performed utilizing binary logistic regression.Results: Participants with moderately severe or severe hearing loss exhibited significant cognitive impairment compared to those with mild hearing loss. It was observed that perceptual dysfunction and depression were independently related to cognitive impairment. However, there was no significant association between somatosensory function, social isolation, and cognitive impairment in the institutionalized older adults.Conclusion: More profound hearing loss, abnormal perception, and depression are associated with cognitive impairment in older adults. Subsequent research endeavors should delve into the causal mechanisms underpinning these associations and explore whether combined interventions have the potential to postpone the onset of cognitive impairment.
作者机构：
[Li, Shang-Ze; Huang, Xi-Cheng; Zhang, Xiao-Dong; Li, Chao; Du, Run-Lei; Chen, Jie] Wuhan Univ, Coll Life Sci, Hubei Key Lab Cell Homeostasis, Wuhan 430072, Hubei, Peoples R China.;[Li, Shang-Ze; Song, Xue-Min] Wuhan Univ, Res Ctr Anesthesiol & Crit Care Med, Zhongnan Hosp, Wuhan 430071, Hubei, Peoples R China.;[Liu, Wenbin] Wuhan Polytech Univ, Coll Hlth Sci & Nursing, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Xue-Min Song] R;[Run-Lei Du] H;Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, Hubei Province, 430072, PR China<&wdkj&>Research Centre of Anesthesiology and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei Province, 430071, PR China
摘要：
Protein tyrosine phosphatase non-receptor type 18 (PTPN18) is often highly expressed in colorectal cancer (CRC), but its role in this disease remains unclear. We demonstrated that PTPN18 overexpression promotes growth and tumorigenesis in CRC cells and that PTPN18 deficiency yields the opposite results in vitro. Moreover, a xenograft assay showed that PTPN18 deficiency significantly inhibited tumorigenesis in vivo. PTPN18 activated the MYC signaling pathway and enhanced CDK4 expression, which is tightly associated with the cell cycle and proliferation in cancer cells. Finally, we found that MYC interacted with PTPN18 and increased the protein level of MYC. In conclusion, our results suggest that PTPN18 promotes CRC development by stabilizing the MYC protein level, which in turn activates the MYC-CDK4 axis. Thus, PTPN18 could be a novel therapeutic target in the future. Copyright (C) 2020, Chongqing Medical University. Production and hosting by Elsevier B.V.
摘要：
目的:分析发表在中国知网（China National Knowledge Infrastructure,CNKI）上的关于我国胃癌患者照顾者研究的发展动态,为今后的深入研究提供参考。方法:以中国知网（CNKI）为数据来源,选择知网下的文献数据库,在输入检索条件下的"主题"字段输入"胃癌"或者"照顾者",在"篇名"字段输入"照顾者"或者"家属","主题"与"篇名"之间用"并且"连接,选择"模糊"词频进行检索,对检索到的第一作者为中国大陆的文献进行分析。结果:共检索出65篇文献,选择符合纳入条件的21篇文献进行研究分析。结论:来自全国各地的研究者都越来越关注胃癌患者的照顾者,其中以描述性研究居多。在今后的研究中应该进一步拓展相关研究的广度与深度,探讨某种合适又高效的干预方式对照顾者进行干预后以达到提高患者的生活质量、促进医患关系和谐的目的。
