摘要：
As the byproduct in fluoride production, fluorogypsum is of slow hydration and poor concreting, playing a role of threat to environmental safety. Microstructure and performances of sludge soil stabilized by fluorogypsum-based cementitious binder were investigated in comparison with the solidified sludge blended with quicklime (LM) and PC32.5 cement (PC32.5) in this paper. The water content (wt%), pH value, unconfined compressive strength (UCS) and strength loss after soaking in water for 24 h of the solidified sludge with different solidifications were discussed. The solidified sludge blended with fluorogypsum-based cementitious binder (FB) possessed the lowest wt% of 9.24% (14 days, solidification agent dosage as 19%) indicating the good compressibility and potential to be a roadbed material. The pH value of 9.67 for FB (14 days, solidification agent dosage as 19%) was closer to neutral than LM and PC32.5. The UCS of FB stabilized for 14 days arrived at 4.7 MPa with the solidification agent dosage as 19%, which was 1.6 and 6.4 times as much as that of PC and LM, respectively, which further proved the good compression performance of FB. SEM and FT-IR demonstrated the formation of ettringite, portlandite and other hydration products, and explained the phenomenon that FB had sufficient early strength. The FB has not only a big specific surface area of 6.02 m(2) g(-1), but also a great pore volume of 2.25 x 10(-2) cm(3) g(-1), which would improve the FB activity and accelerate hydration reaction. Based on the above characterization and analysis, the FB solidified sludge soil was clarified as a roadbed material and potential adsorbent.
作者机构：
[Li, Xun; Ning, Na; Yang, Xiang-Ping; Xia, Minghui; Wang, Jing; Ba, Hongping; Liu, Huicheng; Weng, Junmei] Huazhong Univ Sci & Technol HUST, Tongji Med Coll, Sch Basic Med, Dept Immunol, Wuhan, Peoples R China.;[Cheng, Xiang; Zhang, Si] HUST, Tongji Med Coll, Union Hosp, Lab Cardiovasc Immunol,Inst Cardiol, Wuhan, Peoples R China.;[Wu, Qi; Wang, Guihua] HUST, Tongji Med Coll, Tongji Hosp, GI Canc Res Inst, Wuhan, Peoples R China.;[Ao, Qilin; Kuang, Dong; Duan, Yaqi; Wang, Guoping] HUST, Tongji Med Coll, Tongji Hosp, Inst Pathol, Wuhan, Peoples R China.;[Ao, Qilin; Kuang, Dong; Duan, Yaqi; Wang, Guoping] HUST, Tongji Med Coll, Sch Basic Med, Dept Pathol, Wuhan, Peoples R China.
关键词：
DLBCL;Venetoclax;AGK;BCL-2;FOXO1
摘要：
Background: Despite of the paradigm change on the treatments of acute myeloid leukemia (AML) and chronic lymphocytic leukemia (CLL) by venetoclax, it has been less successful in the treatment of diffuse large B-cell lymphoma (DLBCL). Here, we explored whether acylglycerol kinase regulates the sensitivity of DLBCLs to venetoclax and its mechanism in both cell lines and preclinical animal models. Methods: The expression of AGK and sensitivity to venetoclax of seven DLBCL cell lines were determined. Upon knockdown and overexpression of AGK by lentivirus in DLBCL cells, the venetoclax-induced apoptosis and PTEN-FOXO1-BCL-2 signaling axis were evaluated in vitro. The efficacy of venetoclax and PTEN-FOXO1-BCL-2 signaling axis were evaluated in immunodeficient NCG mice that were implanted with control or shAGK stably transduced SU-DHL4 cells. The expressions of AGK, BCL-2 and FOXO1 were evaluated in tumor tissues of DLBCL patients. Results: AGK expression was inversely correlated with sensitivity of DLBCL to venetoclax. Inhibition of AGK rendered the DLBCL cells more sensitive to venetoclax. Mechanistically, AGK phosphorylated and inactivated PTEN, which led to AKT activation and reduced FOXO1 nuclear translocation. Inhibition of AGK also led to enhanced efficacy of venetoclax for suppression of DLBCL tumor growth in vivo, which was dependent on FOXO1. In human DLBCL tumor tissues, the expression of AGK inversely correlated with BCL-2 expression, as well as the amounts of nuclear FOXO1. Conclusions: Our data demonstrated that AGK regulates venetoclax response in DLBCL via PTEN-FOXO1-BCL-2 signaling axis. Targeting AGK may enhance the efficacy of venetoclax for the treatment of DLBCL patients.
作者机构：
[Tang, Mingwei] Chengdu Med Coll, Dept Endocrinol & Metab, Clin Med Coll, Affiliated Hosp 1, Chengdu, Peoples R China.;[Wang, Fengbo; Wang, Ke; Jiang, Yuan; Wang, Qiongfen] Chengdu Med Coll, Clin Med Coll, Dept Rehabil Med, Affiliated Hosp 1, 278 Middle Sect Baoguang Ave, Chengdu 610500, Sichuan, Peoples R China.
通讯机构：
Department of Rehabilitation Medicine, Clinical Medical College of Chengdu Medical College, First Affiliated Hospital of Chengdu Medical College, No. 278, Middle Section of Baoguang Avenue, Xindu District, Sichuan, Chengdu, China
关键词：
cell, circ_0058063;miR-330-3p;SDC4;thyroid cancer
摘要：
Circular RNA takes a crucial part in carcinogenesis. Circ_0058063 has been found to act as an oncogene in esophageal cancer and bladder cancer, but its role in thyroid cancer (TC) is still under investigation. Therefore, we carried out a study to understand its role in TC and its association with miR-330-3p. The circ_0058063 and miR-330-3p in TC tissues and cells were quantified by quantitative reverse transcription PCR, and cell counting kit-8 and scratch adhesion test were conducted for evaluation of cell proliferation and migration. In addition, a dual luciferase reporter assay and RNA immunoprecipitation assay were conducted for interaction analysis between circ_0058063 and miR-330-3p. Circ_0058063 was upregulated in TC tissues and cells, but miR-330-3p expression showed an opposite trend. Both silencing circ_0058063 and upregulating miR-330-3p can suppress the proliferation and migration of TC cells, upregulate Bax, and downregulate Bcl-2. In addition, circ_0058063 is able to target miR-330-3p that is also able to target syndecan 4 (SDC4). circ_0058063 can act as a carcinogen in cases with TC via the miR-330-3p/SDC4 axis.
作者机构：
[Xie, Jun; Jin, Xiaogang; Cai, Ruifan; Jin, XG] Zhejiang Univ, State Key Lab CAD&CG, Hangzhou 310058, Peoples R China.;[Li, Honglin] Quanzhou Med Coll, Quanzhou 362000, Peoples R China.
通讯机构：
[Jin, XG ] Z;Zhejiang Univ, State Key Lab CAD&CG, Hangzhou 310058, Peoples R China.
关键词：
Accurate indoor floorplan reconstruction;Super-boundary-point;Geometric priors;Approximate optimal path
摘要：
We present an accurate and automatic bottom-up floorplan reconstruction method by leveraging geometric priors extracted from raw point clouds of indoor scenes. Compared to two state-of-theart methods which adopt point density as priors only, our designed geometric priors integrate point density with indoor area recognition and normal information. These geometric priors are used to calculate the confidence score for each unit region as part of the external boundaries. A cost function is developed according to the confidence scores and the normals along a certain edge, as well as the edge length. By minimizing the cost function, we can automatically estimate candidate edges, and connect them edge by edge to generate rough external boundaries. After diminishing the redundant edges, we can finally solve for a fine closed-loop external boundary path via an efficient heuristic method. We validate our method on intensive real indoor scenes. Experimental results show that our method outperforms two state-of-the-art methods in terms of reconstructing accurate external floorplan boundaries.(C) 2021 Elsevier Ltd. All rights reserved.
摘要：
Journal of Low Frequency Noise, Vibration and Active Control, Ahead of Print. <br/>Chaotic systems arise everywhere in control theory and nonlinear vibration. This paper uses a high-precision numerical approach for capturing chaotic attractors of the fractional EI Niño chaotic systems. The numerical results indicate that some of the chaotic attractors of fractional-order systems are topologically equivalent to those discovered in the integer-order systems, and some unusual attractors of fractional EI Niño chaotic systems are found by using the present method.
摘要：
PURPOSE We aimed to assess the feasibility of radiomics analysis based on non-contrast-enhanced thoracic CT images in predicting synchronous brain metastasis (SBM) in lung cancer patients at initial diagnosis. METHODS This retrospective study enrolled 371 lung cancer patients (with SBM n=147, without SBM n=224) confirmed by histopathology. Patients were allocated to the training set (n=258) and testing set (n=113). The optimal radiomics features were selected by using the least absolute shrinkage and selection operator (LASSO) algorithm. The radiomics, clinicoradiologic, and combined models were developed to predict SBM using multivariable logistic regression. Then the discrimination ability of the models was assessed. Furthermore, the prediction performance of the abovementioned three models for oligometastatic (1-3 lesions) or multiple (>3 lesions) brain metastases in SBM, metachronous brain metastasis (MBM), and total (SBM and MBM) groups were investigated. RESULTS Six radiomics features and two clinicoradiologic characteristics were chosen for predicting SBM. Both the radiomics model (area under the receiver operating characteristic curve [AUC] = 0.870 and 0.824 in the training and testing sets, respectively) and the combined model (AUC = 0.912 and 0.859, respectively) presented better predictive ability for SBM than the clinicoradiologic model (AUC = 0.712 and 0.692, respectively). The decision curve analysis (DCA) demonstrated the clinical usefulness of the radiomics-based models. The radiomics model can also be used to predict oligometastatic or multiple brain metastases in SBM, MBM, and total groups (P =.045, P =.022, and P =.030, respectively). CONCLUSION The radiomics model and the combined model can be used as valuable imaging markers for predicting patients at high risk of SBM at the initial diagnosis of lung cancer. Furthermore, the radiomics model can also be utilized as an indicator for identifying oligometastatic or multiple brain metastases.
摘要：
The large-scale model test bench frame is adopted to carry out shear test on the pre-stressed anchor block containing structural plane, and the characteristics of tensile, bending and shear failure of anchor cable are analyzed. Furthermore, the structural calculation software ABAQUS 2020 is utilized to construct the spiral structure model of composite steel wires and the anchor cable is simplified to be composed of bending inner and outer steel wires and central steel wire, so as to simulate accurately the interaction, failure mode and mechanical mechanism between anchor cable and grouting body, as well as between grouting body and concrete block. The results show that the anti-shearing process of pre-stressed anchor blocks can be divided into the anti-shearing stage of structural plane and the anti-shearing stage of anchor cable. Affected by the anti-shearing and deformation characteristics of the bending inner and outer steel wires, the shear stress of the anchor cable has experienced an alternate change process from strong to weak, then from weak to strong, and finally to weak. The axial force of the anchor cable continuously increases until the fracture of the anchor cable. Anchor cable fracture first appears at plastic hinge position of the inner bending steel wire, and then at the interface between the anchor cable and the structural plane, the central steel wire occurs tensile-shear fracture.
期刊：
International Journal of Modern Physics B,2022年36(09N11) ISSN：0217-9792
通讯作者：
Li Zhang
作者机构：
[Ren, Ruxin; Ma, Shijie; Zhang, Li; Lu, Huihu; Liu, Chengzhi; Li, Taotao] North Univ China, Sch Mech Engn, Taiyuan 030051, Peoples R China.;[Feng, Shixin] Taiyuan Tech Coll, Taiyuan 030021, Peoples R China.;[Zhang, Li; Zhang, Yaohui; Fan, Wei] North Univ China, Sch Energy & Power Engn, Taiyuan 030051, Peoples R China.;[Li, Guoping] Taiyuan Iron & Steel Co Ltd, Taiyuan 030003, Peoples R China.;[Zhou, Diaoyu; Liu, Chengzhi] North Univ China, Coll Mat Sci & Engn, Taiyuan 030051, Peoples R China.
会议名称：
1st China-New Zealand Forum on Advanced Materials and Processing Technology (AMPT) / 5th International Forum on Advanced Materials and Processing Technoogies
会议时间：
MAY 28-29, 2021
会议地点：
Jiangsu Univ Sci & Technol, Zhenjiang, PEOPLES R CHINA
摘要：
Corrosion resistance is of practical importance for the application of 2205 duplex steels in chemical industries. For taking an insight into pitting corrosion behavior, electrolysis was employed to distinguish the 2205 duplex steel samples. In the following, X-ray diffraction, scanning electron microscopy and electron backscattering diffraction are used to characterize the regions with superior and inferior corrosion performance, thus constructing the relationship between the microstructure and their corrosion behavior.
期刊：
Journal of Cleaner Production,2022年371:133573 ISSN：0959-6526
作者机构：
[Xiao, Liehui; Huang, Si-Min; Yang, Minlin] Dongguan Univ Technol, Guangdong Prov Engn Res Ctr Distributed Energy Sys, Guangdong Prov Key Lab Distributed Energy Syst, Dongguan 523808, Peoples R China.;[Yang, Yu] Dongguan City Coll, Dongguan 523419, Peoples R China.;[Han, Zhonghe; Wu, Di] North China Elect Power Univ, Sch Energy Power & Mech Engn, Dept Power Engn, Baoding 071003, Peoples R China.
关键词：
Transport membrane condenser;Condensate water;Water and heat recovery;Performance study;Technical and economic analysis
摘要：
Transport membrane condenser (TMC) is an effective equipment for water and heat recovery from flue gas exhausted by coal-fired power plants. Characteristic of cooling water source for TMC is crucial to design and performance of the device. Due to the high water quality and relatively large water flow rate, the condensate water from the unit condenser is a suitable water source. However, the water flow rate in related research ex-ceeds the range of condensate water flow rate, and the results cannot be applied in this condition. Thus, we established a numerical model for performance study of TMC. The condensate water from the condenser of a 600 MW coal-fired power unit is adopted. The effects of structural and operating parameters on the performance of TMC are investigated, and the technical and economic analysis is conducted. Results show that series connection of membrane modules is more suitable than parallel connection for engineering applications. Moreover, the number of transverse rows should be larger than 60 by comprehensively considering heat and water recovery performance and flow resistance. Due to the high benefit of recovered heat, the maximum payback period is 1.68 years. This study provides theoretical support for the engineering application of TMC using condensate water as the cooling water source.
期刊：
Mathematical Problems in Engineering,2022年2022 ISSN：1024-123X
作者机构：
[Yi, ChongYu] MuDanJiang Normal Univ, Fac Sports & Hlth Sci, Mudanjiang 157011, Heilongjiang, Peoples R China.;[Liu, Yongqiang] Harbin Inst Technol, Dept Sports, Harbin, Heilongjiang, Peoples R China.;[Zhiqiang, Zhao] Handan Polytech Coll, Dept Exercise & Hlth, Handan 056001, Peoples R China.
摘要：
The conventional college snow and ice teaching multi-source resource intelligent management system has the problem of incomplete software resource analysis function, which leads to high CPU usage of system. A cloud platform based college snow and ice teaching multi-source resource intelligent management system is designed. Hardware part: record the state of the pins when the register is reset, account for the data bit width and storage capacity of DDR2 SDRAM memory, and optimise the data storage module; Part of the software: obtain ice and snow sports course objectives in colleges and universities, rationally organise the course organization form, optimise the intelligent management mode of teaching multi-source resources by using the cloud platform, support online browsing of various text resources, set relevant parameters to construct various random modification operations, and design the software resource analysis function with the knowledge fusion algorithm. Experimental results: The average CPU usage of the multi-source intelligent resource management system for snow and ice teaching in colleges and universities in this paper and the other two systems is 34.257%, 47.458%, 53.578%. Experimental results show that the performance of multi-source intelligent resource management system for snow and ice teaching in colleges and universities has been significantly improved after making full use of cloud platform.
作者机构：
[Wang, Yanli; Sun, Yuanwei; Li, Cui; Dai, Xiaodong] Shandong Inst Petr & Chem Technol, Coll Petr Engn, Dongying 257061, Peoples R China.;[Sun, Dexu] Sinopec, Shengli Oilfield Branch, Binnan Oil Prod Plant, Binzhou 251700, Peoples R China.;[Zhao, Yizhong] Shengli Oilfield Petr Dev Ctr Co Ltd, Dongying 257000, Peoples R China.;[Gao, Yeping] Dongying Vocat Coll, Sch Petr & Chem Engn, Dongying 257091, Peoples R China.;[Sun, Yuanwei; Cheng, Yuanfang] China Univ Petr East China, Sch Petr Engn, Qingdao 266580, Peoples R China.
摘要：
Open hole combined with a sand filter pipe is the main development mode of an unconsolidated sandstone horizontal well, which causes problems such as shaft wall collapse, sand filter pipe blockage, and even damage. At present, the polyurethane intelligent sand control screen pipe can effectively solve these problems, but its application scope is limited by the downhole temperature and material strength. Therefore, the high-strength shape memory polyphenylene sulfide (SMPPPS) was synthesized by an indoor experiment, then the shape memory performance was analyzed, and the variation law of pore size with composition and processing technology was obtained. Finally, the performance of the SMPPPS intelligent screen was evaluated. It is found that (1) with the increase of the cross-linking agent content, the melt index and melt peak temperature of SMPPPS decrease rapidly at first and then remain unchanged; the cross-linking agent content is set at 4.5 wt %. (2) The shape memory performance of SMPPPS is good, and the recovery ratio can reach 99.8% at 110 degrees C in 11.8 min. (3) The relationship between the pore size distribution proportion of SMPPPS and the foaming agent content, pore enhancer content, temperature, and pressure is determined through experiments; the main distribution range of pore size increases with the increase of the foaming agent content and temperature and decreases with the increase of the pore enhancer content and pressure. When the foaming agent content is 0.5 wt %, the pore enhancer concentration is 10 wt %, the molding temperature is 320 degrees C, the molding pressure is 10 MPa, and the main distribution range of pore size is 80-320 mu m. (4) The permeability of recovery SMPPPS with a compression ratio of 300% first increases slightly and then decreases slightly with the increase of flow, with a variation range of 420.5-463 mD; the compressive strength range is 9.5-11.4 MPa; the SMPPPS has good adaptability to downhole fluid; the sand retaining accuracy is higher than that of the screen sand filter pipe, but the blockage is more serious.
摘要：
Journal of Engineered Fibers and Fabrics, Volume 17, Issue , January-December 2022. <br/>In this work, the effects of degumming treatment on the structure and properties of OS-BADF were discussed. Alkali degumming and biological enzyme degumming were used to obtain AD-BADF and BED-BADF respectively. Spinnability index, thermal property, macromolecular structure, bending property and antibacterial property of OS-BADF (Original Sample of Bast Fiber of Alchornea Davidii Franch), AD-BADF (Alkali Degummed Bast Fiber of Alchornea Davidii Franch), and BED-BADF (Biological Enzyme degummed Bast Fiber of Alchornea Davidii Franch) were tested. The surface morphology of the above three kinds of fiber was analyzed by SEM. The results showed that the main component of degummed OS-BADF was cellulose, and the fiber had stripes in longitudinal direction, and kidney ellipsoid, irregular polygon in the cross section. After degumming treatment, the fineness of fiber decreased obviously and reached the spinnability index. Non-cellulose substances in OS-BADF can be removed by degumming. SEM observation showed that AD-BADF and BED-BADF became smoother after degumming. Compared with AD-BADF, BED-BADF had finer linear density, better thermal stability and higher antibacterial property. The equivalent flexural modulus and flexural rigidity of AD-BADF and BED-BADF were decreased compared with OS-BADF. In particular, the equivalent flexural modulus and the flexural rigidity of BED-BADF were mostly reduced, which indicated that BED-BADF became thinner and softer. The initial thermal decomposition temperature and the highest thermal decomposition temperature of AD-BADF and BED-BADF had been significantly increased. And -OH (free group) decreased significantly after degumming treatment. The testing results of the antibacterial properties of the three blended fabrics showed that the antibacterial properties of AD-BADF and BED-BADF were improved. Compared with AD-BADF, the antibacterial properties of BED-BADF were improved significantly. On the one hand, this research can provide a basis for technological research for the development of ADF (Alchornea Davidii Franch) bast fiber blended yarn and its textile product development. On the other hand, because of the high mechanical properties of ADF bast fiber, it also provides new development idea for the development of natural high-performance fiber reinforcement composites.
摘要：
Insulin glargine is a long-acting insulin analog, which plays an important role in the treatment of diabetes mellitus. Biosimilar products of insulin glargine can provide patients with additional safe, high-quality, and potentially cost-effective options for treating diabetes. This article presents a randomized, double-blind, single-dose, two-treatment, four-period, replicate crossover, euglycemic clamp study which was designed to evaluate the PK and PD similarity between the recombinant insulin glargine developed by Wanbang (test) and Lantus(R) (reference) in healthy volunteers. Subjects received subcutaneous administration of the insulin glargine formulation (0.4 U/kg) on two occasions for the test and reference drug, respectively, and a 20% dextrose solution was infused at variable rate to clamp the blood glucose concentrations at 0.3 mmol/L below the subjects' fasting glucose for 24 h. Taking advantage of the improved sensitivity of the bioanalytical method applied and the solution of the matrix stability problem, the parent insulin glargine was determined in the vast majority of plasma samples using a fully validated UHPLC-MS/MS method. The PK characteristics of the parent insulin glargine were revealed for the first time: after subcutaneous injection, concentrations of the parent insulin glargine increased to a relative high level within 3 h, and then, a relatively flat concentration-time profile lasting for at least 12 h post-dose was observed. For the first time, the pharmacokinetic parameters of the parent insulin glargine were used as endpoints for similarity evaluation, which complied with the regulatory guidance better and made the similarity conclusion more powerful. The ratios of geometric means of all PK and PD endpoints were close to 100.00%. For the PK endpoints (AUC(0-24h), C-max,C- AUC(0-12h), and AUC(12-24h) of the parent insulin glargine and its metabolite M1), the 90% confidence intervals of geometric mean ratios of test to reference were entirely contained within 80.00%-125.00%. For the PD endpoints [AUC(GIR(0-24h)), GIR(max,) AUC(GIR(0-12h)), and AUC(GIR(12-24h))], the 95% confidence intervals of geometric mean ratios of test to reference were entirely contained within 80.00%-125.00%. Based on the above mentioned results, it can be concluded that the PK and PD characteristics of the biosimilar drug developed by Wanbang are similar to those of Lantus.
作者机构：
[Xu, Xiaohui] Warman Med Coll, Sch Preclin Med, Wuhu 241002, Peoples R China.;[Yang, Qianwen; Zhu, Yunyi; Yue, Changling; Xu, Xiaohui] Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China.;[Zhang, Zhaohuan] Naval Med Univ, Changzheng Hosp, Dept Lab Med, Shanghai 200003, Peoples R China.
关键词：
bioinformatics;transcription factors;reverse docking;daphnoretin;JAK2 agonist;JAK2/STAT3;Chinese medicine monomer;NSCs;OPCs;multiple sclerosis
摘要：
How to use bioinformatics methods to quickly and accurately locate the effective targets of traditional Chinese medicine monomer (TCM) is still an urgent problem needing to be solved. Here, we used high-throughput sequencing to identify the genes that were up-regulated after cells were treated with TCM monomers and used bioinformatics methods to analyze which transcription factors activated these genes. Then, the binding proteins of these transcription factors were analyzed and cross-analyzed with the docking proteins predicted by small molecule reverse docking software to quickly and accurately determine the monomer’s targets. Followeding this method, we predicted that the TCM monomer Daphnoretin (DT) directly binds to JAK2 with a binding energy of −5.43 kcal/mol, and activates the JAK2/STAT3 signaling transduction pathway. Subsequent Western blotting and in vitro binding and kinase experiments further validated our bioinformatics predictions. Our method provides a new approach for quickly and accurately locating the effective targets of TCM monomers, and we also have discovered for the first time that TCM monomer DT is an agonist of JAK2.
摘要：
Combinations of Tibetan Tea and Medicine Food Homology Herbs, A new Strategy for Obesity Prevention. Abstract Obesity has become a significant global public health problem. Functional drinks have been an essential direction for obesity prevention research. The present study investigated the preventive effect and safety of winter melon and lotus leaf Tibetan tea (WLTT, a compound tea drink based on Ya'an Tibetan Tea and medicine food homology herbs) on obesity. The rats' hypercaloric high‐fat diet (HFD) obesity model was established to evaluate obesity prevention and explored the mechanism through intestinal flora regulation. The results showed that in obese rats with the intervention of WLTT (400, 800, and 1600 mg/kg BW), the body weight, fat accumulation, adipocyte cell size, serum lipid levels, and antioxidant enzyme activity (SOD, GSH‐Px, and MDA) were progressively improved. 16S rRNA high‐throughput sequencing showed that WLTT could improve intestinal flora disorders due to HFD, which significantly reversed the relative abundance of Firmicutes and the F/B ratio associated with an HFD, and significantly upregulated the relative abundance of Verrucomicrobia. At the genus level, the downregulation of the relative abundance of Akkermansia and unclassified_Lachnospiraceae groups, and the upregulation of the relative abundance of Romboutsia, Ruminococcus, Corynebacteriume, and Saccharibacteria_genera_incertae_sedis groups brought about by the HFD were significantly reversed. The results of the above experiments were compared favorably with those of a parallel experiment with Bi ‐Sheng ‐Yuan slimming tea (BSY, a functional drink based on green tea and medicine food homology herbs). Overall, the findings have provided that WLTT can prevent obesity owing to an HFD by regulating intestinal flora and has a good safety profile, and combinations of Tibetan tea and medicine food homology herbs could be a new option for obesity prevention.
