作者：
An, Jiejie;Liu, Mingzhu;Din, Zia-ud;Xie, Fang*;Cai, Jie
期刊：
International Journal of Biological Macromolecules,2023年247:125697 ISSN：0141-8130
通讯作者：
Xie, Fang;Cai, J
作者机构：
[Cai, Jie; An, Jiejie; Liu, Mingzhu; Xie, Fang] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Women Univ Swabi, Dept Food Sci & Nutr, Swabi 23430, Khyber Pakhtunk, Pakistan.;[Cai, Jie; Xie, F] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J ; Xie, F] ;Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Delivery;Nanogels;Starch
摘要：
Nanogels formed by self-assembly of natural proteins and polysaccharides have attracted great interest as potential carriers of bioactive molecules. Herein, we reported that carboxymethyl starch-lysozyme nanogels (CMS-Ly NGs) were prepared using carboxymethyl starch and lysozyme by green and facile electrostatic self-assembly, and the nanogels served as epigallocatechin gallate (EGCG) delivery systems. The dimensions and structure of the prepared starch-based nanogels (i.e., CMS-Ly NGs) were characterized by dynamic light scattering (DLS), ζ-potential, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermal gravimetric analyzer (TGA). FT-IR and (1)H NMR spectra together confirmed the formation of CMS; FT-IR spectra confirmed the formation of CMS-Ly NGs; XRD spectra confirmed the disruption of the crystal structure of lysozyme after electrostatic self-assembly with CMS, and further confirmed the formation of nanogels. TGA demonstrated the thermal stability of nanogels. More importantly, the nanogels showed a high EGCG encapsulation rate of 80.0±1.4%. The CMS-Ly NGs encapsulated with EGCG exhibited regular spherical structure and stable particle size. Under the simulated gastrointestinal environmental conditions, CMS-Ly NGs encapsulated with EGCG showed the controlled release potential, which increased its utilization. Additionally, anthocyanins can also be encapsulated in CMS-Ly NGs and showed slow-release properties during gastrointestinal digestion in the same way. Cytotoxicity assay also demonstrated good biocompatibility between CMS-Ly NGs and CMS-Ly NGs encapsulated with EGCG. The findings of this research suggested the potential application of protein and polysaccharides-based nanogels in the delivery system of bioactive compounds.
作者机构：
[Liu, Yan; Meng, Shizhen; Li, Ming; Gong, Zhiyong; Wu, Yongning; Wang, Qiao; Yu, Qingqing; Liu, Xin; Zhao, Xiaole] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod,Minist E, Wuhan 430023, Peoples R China.;[Wu, Yongning] Chinese Acad Med Sci, China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Food Safety Res Unit 2019RU014, Beijing 100021, Peoples R China.;[Wu, Kejia] Jiangnan Univ, Wuxi Sch Med, Wuxi, Jiangsu, Peoples R China.;[Liu, Xin] 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, X ] 6;68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
Human serum albumin;Ionic liquid;Protein binding;Toxicology implication
摘要：
Alkyl imidazolium-based ionic liquids (ILs) are promising for diverse industrial applications; however, their growing prevalence has raised concerns regarding human exposure and potential health implications. A critical aspect to be clarified to address the adverse health effects associated with ILs exposure is their binding mode to human serum albumin (HSA). In this study, we delved into the binding interactions between three alkyl imidazolium ILs (1-hexyl-3-methyl-imidazolium (C6[MIM]), 1-ethyl-3-methyl-imidazolium chloride (C8[MIM]) and 1-decyl-3-methyl-imidazolium (C10[MIM]) and human serum albumins (HSAs) using a comprehensive approach encompassing molecular docking and multi-spectroscopy (UV-visible, Fluorescence, Circular Dichroism, FTIR). Furthermore, for the first time, we developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach time to quantify plasma protein binding rates. Our results revealed that the ILs primarily bind to the hydrophobic cavity of HSA through hydrogen bonding and van der Waals forces, forming stable complexes via static quenching. This affected HSA's secondary structure, reducing α-helical content, particularly around specific residues. Equilibrium dialysis and ultrafiltration coupled with UPLC-MS/MS analysis showed modest plasma protein binding rates (17.84%-31.85%) for the three ILs, with no significant influence from alkyl chain effects or concentration relationship. Lower plasma protein binding rates can affect bioavailability and distribution of ILs, potentially influencing their toxicity. These findings provide critical insights into the potential toxicological implications at the molecular level, thereby contributing to continuous efforts to evaluate the risk profiles and ensure the safe utilization of these compounds.
通讯机构：
[Liming Cheng; Hao-Long Zeng] D;Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
摘要：
BACKGROUND: Trace metals/metalloids were important for the biological functions of both the eukaryotic host and the microorganism. Their concentrations and variations may associate with the critical illness in sepsis, which still needs to be investigated. METHODS: We performed a prospective cohort study on the patients with sepsis admitted to Tongji hospital (Wuhan, China) from Jul 01 to Dec 31, 2021. Sepsis was diagnosed in accordance with the third international consensus definitions for sepsis and septic shock (Sepsis-3). The concentrations of metals/metalloids including magnesium (Mg), calcium (Ca), chromium (Cr), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), mercury (Hg), thallium (Tl) and lead (Pb) in whole blood were analyzed by ICP-MS based methods. RESULTS: Compared to the healthy controls, patients with sepsis showed higher levels of Ca, Cr and Cu, and lower levels of Mg, Mn, Fe, Zn, As, Hg and Pb. Further analysis between the critical illness and noncritical illness, revealed the Mn, Fe were significantly lower in the critically-ill sepsis. The longitudinal profile of the two metals show the differences appeared to exist almost throughout the clinical course. By performing the binary regression logistic analysis, we determined the Fe, Mn as independently risk factors for critical illness in sepsis, with effect sizes (β) of 17.14 (95%CI: 1.79-163.81) and 10.83 (1.96-59.83), respectively, which collectively discriminated 83.3% of all cases between critical-illness and non-critically illness. CONCLUSIONS: The variations of whole blood metals/metalloids were associated with the critically-ill sepsis.
期刊：
Environmental Science and Pollution Research,2023年30(36):86232-86243 ISSN：0944-1344
通讯作者：
Qi, BK
作者机构：
[Zhu, Zhenzhou] Wuhan Polytech Univ, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Wu, Sirong] Wuhan Polytech Univ, Coll Food Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Wu, Sirong; Qi, Benkun; Luo, Jianquan] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China.;[Wang, Caixia] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China.;[Wan, Yinhua; Luo, Jianquan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
通讯机构：
[Qi, BK ] C;Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China.
作者机构：
[Xiao, Anhong; Duan, Shuo; Liu, Xiaodan; Shu, Zaixi; Dai, Huang; Wang, Jiahua; Yuan, Zhenhong] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Dai, Huang] Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Wuhan 430023, China. Electronic address: huangdai9@126.com;[Xiao, Anhong; Duan, Shuo; Liu, Xiaodan; Shu, Zaixi] Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Wuhan 430023, China;[Shen, Yafang] Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;[Zhang, Qi] School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China
通讯机构：
[Dai, H.] C;College of Food Science and Engineering, Hubei province, China
作者：
Zhuo, Yue;Liang, Yuehui;Xu, Duoxia;McClements, David Julian;Wang, Suqing;...
期刊：
International Journal of Biological Macromolecules,2023年242(Pt 4):125109 ISSN：0141-8130
通讯作者：
Chen, S
作者机构：
[Zhuo, Yue; Chen, Shuai; Liang, Yuehui] Wuhan Univ, Sch Publ Hlth, Wuhan 430071, Peoples R China.;[Xu, Duoxia] Beijing Technol & Business Univ, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.;[McClements, David Julian] Univ Massachusetts Amherst, Dept Food Sci, Amherst, MA 01003 USA.;[Wang, Suqing] Wuhan Univ, Sch Nursing, Wuhan 430071, Peoples R China.;[Li, Qi] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Neurosurg, Wuhan 430022, Peoples R China.
通讯机构：
[Chen, S ] W;Wuhan Univ, Sch Publ Hlth, 115,Donghu Rd, Wuhan 430071, Peoples R China.
关键词：
Chondroitin sulfate;Quercetagetin;Zein
摘要：
Zein-quercetagetin-chondroitin sulfate (Zein-Que-CS) composite nanoparticles with different compositions were successfully fabricated using a novel antisolvent co-precipitation method. The mean particle diameter (97.5 to 219.4nm), negative surface potential (-29.9 to -51.1mV), and turbidity (265 to 370 NTU) of suspensions of Zein-Que nanoparticles increased after the addition of CS. Electrostatic attraction, hydrogen bonding, and hydrophobic attraction were the main driving forces for the formation of the composite nanoparticles. The encapsulation efficiency and loading capacity of the quercetagetin within the Zein-Que-CS (100:10:30) nanoparticles were 91.6% and 6.1%, respectively. The photostability and thermal stability of the encapsulated quercetagetin were 3.4- and 3.2- fold higher than that of the free form. The nanoparticles had good resistance to sedimentation and exhibited slow-release properties under simulated gastrointestinal conditions. The Zein-Que-CS nanoparticles developed in this study may therefore be useful for the encapsulation, protection, and delivery of quercetagetin.
期刊：
International Journal of Biological Macromolecules,2023年225:557-564 ISSN：0141-8130
通讯作者：
Chengdeng Chi<&wdkj&>Youcai Zhou
作者机构：
[Chi, Chengdeng; He, Yongjin; Xiao, Xuehua; Zhou, Youcai; Liu, Pingying; Chen, Bilian] Fujian Normal Univ, Coll Life Sci, Fuzhou 350117, Peoples R China.;[He, Yongjin; Chen, Bilian] Fujian Normal Univ, Engn Res Ctr Ind Microbiol, Minist Educ, Fuzhou 350117, Peoples R China.;[Tan, Xiaoyan] Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Peoples R China.;[Ji, Zhili] Wuhan Polytech Univ, Sch Food Sci & Technol, Cereal Engn, Wuhan 430023, Peoples R China.;[Zhang, Yiping] Henan Normal Univ, Coll Life Sci, Henan Engn Lab Bioconvers Technol Funct Microbes, Xinxiang 453007, Peoples R China.
通讯机构：
[Chengdeng Chi; Youcai Zhou] C;College of Life Sciences, Fujian Normal University, Fuzhou 350117, China
关键词：
Microalgae;Starch functionalities;Starch structure;Very small granular starch;chlorella starch
摘要：
Novel resources of very small granular starch are of great interests to food scientists. We previously found Chlorella sp. MBFJNU-17 contained small granular starch but whether the MBFJNU-17 was a novel resource of very small granular starch remained unresolved. This study isolated and characterized the starch from MBFJNU-17 in comparison with quinoa starch (a typical very small granular starch), and discussed whether the MBFJNU-17 could be a resource of very small granular starch. Results showed that chlorella starch displayed a smaller size (1024 nm) than quinoa starch did (1107 nm), suggesting MBFJNU-17 was a good resource of very small granular starch. Additionally, chlorella starch had less amylose, higher proportion of long amylopectin branches, more ordered structures, thinner amorphous lamellae, better paste thermostability, and slower enzymatic digestion than quinoa starch did. These findings indicated that Chlorella sp. MBFJNU-17 was a novel resource of very small granular starch with desirable thermostability and nutritional attributes.
通讯机构：
[Aixi Yu] D;[Ang Lu] C;Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China<&wdkj&>College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan 430072, China
摘要：
Trade-off of high-strength and dynamic crosslinking of hydrogels remains an enormous challenge. Motivated by the self-healing property of biological tissues, the strategy of combining multiple dynamic bond mechanisms and a polysaccharide network is proposed to fabricate biomimetic hydrogels with sufficient mechanical strength, injectability, biodegradability, and self-healing property for bone reconstruction engineering. Stable acylhydrazone bonds endowed hydrogels with robust mechanical strength (>10 kPa). The integration of dynamic imine bonds and acylhydrazone bonds optimized the reversible characteristic to protect the cell during the injection and mimicked ECM microenvironment for cell differentiation as well as rapid adapting bone defect area. Furthermore, due to the slow enzymatic hydrolysis kinetics of chitosan and the self-healing properties of resulting networks, hydrogels exhibited a satisfactory biodegradation period (>8 weeks) that highly matches with bone regeneration. Additionally, rBMSC-laden hydrogels exhibited splendid osteogenic induction and bone reconstruction without prefabrication scaffolds and incubation, demonstrating tremendous potential for clinical application. This work proposes an efficient strategy for the construction of a low-cost multifunctional hydrogel, making polysaccharide-based hydrogels as the optimal carrier for enabling cellular functions in bone repair.
关键词：
L. plantarum;Metabolomics;Microbial interaction;S. cerevisiae;Suanyu;Transcriptomics
摘要：
S. cerevisiae and L. plantarum play important roles in Suanyu fermentation. This study investigated the interaction between S. cerevisiae and L. plantarum during fermentation and its impact on metabolic pathways. Co-culturing S. cerevisiae and L. plantarum increased pH to 5.72, reduced TVB-N to 9.47 mg/mL, and achieved high utilization rates of sugars (98.9%) and proteins (73.7%). During microbial interactions, S. cerevisiae and L. plantarum produced antibiotics, including phenyllactate and Gentamicin C1a, inhibiting the growth of each other. S. cerevisiae used S-adenosyl-l-methionine to counteract acid production of L. plantarum, establishing dominance in Suanyu fermentation. Microbial interactions influenced carbohydrate and energy metabolism pathways, such as nicotinate and nicotinamide metabolism and purine metabolism. S. cerevisiae significantly impacted gene expression in protein synthesis and cell growth pathways, including ribosome, SNARE interactions, basal transcription factors, and MAPK signaling. These findings offer insights into microbial interactions and metabolic processes during Suanyu fermentation.
摘要：
The objective of this study was to examine the pharmacokinetic (PK) properties of enrofloxacin (EF) and its metabolite, ciprofloxacin (CF), in yellow catfish (Pelteobagrus fulvidraco) after a single oral dose of EF at 20 mg/ kg at 20, 25, and 30 degrees C. Samples were collected at pre-designed time points and determined by high-performance liquid chromatography with a fluorescent detector. Results showed that most concentrations of EF and CF in plasma and tissues at the same time point at different temperatures were statistically significant. With the increase in temperature, the terminal half-life (T-1/2 lambda z) of EF and CF was first reduced from 20 to 25 degrees C but elevated from 25 to 30 degrees C in plasma, muscle + skin, gill, liver, and kidney, respectively. The area under the plasma concentration-time curves (AUC(last)) of EF were all decreased in plasma, muscle + skin, and gill except for that of EF in the liver and kidney. However, the AUC(last) and the apparent metabolic rate of CF were exhibited first elevated and then decreased trend. The apparent volume of distribution (Vz_F) of EF was first reduced from 20 to 25 degrees C but increased at 30 degrees C. The apparent total body clearance (CL_F) of EF was increased from 0.15 to 0.32 L/ h center dot kg with the temperature elevation. These indicated that increased temperature markedly affected the PKs of EF and CF in yellow catfish. Through in-depth analysis, the EF dosage of 20 mg/kg is appropriate to use in yellow catfish at 20 and 25 degrees C but 30 degrees C.
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2023年71(18):6987-6998 ISSN：0021-8561
通讯作者：
Lin Zheng<&wdkj&>Mouming Zhao
作者机构：
[Zhang, Qi] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Zhao, Mouming; Luo, Donghui] Chem & Chem Engn Guangdong Lab, Chaozhou Branch, Chaozhou 521000, Peoples R China.;[Zhao, Mouming; Zheng, Lin] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China.
通讯机构：
[Lin Zheng; Mouming Zhao] S;School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China<&wdkj&>Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, China<&wdkj&>School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
摘要：
WCPFSRSF, an octapeptide (Trp-Cys-Pro-Phe-Ser-Arg-Ser-Phe), has been reported to improve memory in mice, but its gastrointestinal stability is unclear. The objective of this study was to evaluate the gastrointestinal stability of peptide WCPFSRSF and explore the neuroprotective potential of its digestive fragments. Results showed that the content of WCPFSRSF after gastric and gastrointestinal digestion decreased to 71.64% and less than 1%, respectively. Furthermore, the antioxidant and neuroprotective ability of WCPFSRSF were also affected. Eleven and nine peptides were identified in its gastric and gastrointestinal digestive products, respectively. Multiple bioinformatics tools in combination with principal component analysis were employed to assess the physicochemical and structural properties of peptides. Novel peptides generated after gastrointestinal digestion could be classified into three groups: the first group had high bioactivity and bioavailability; the second group had high amphiphilicity, charge, and net hydrogen; and the third group had a long peptide chain. In addition, the representative peptides WCPF and SR showed neuroprotective ability.
摘要：
Metabolic syndrome (MetS) is a global health problem, and EPA/DHA-enriched phospholipids (EPA/DHA-PLs) have been found to have positive effects on MetS improvement. Currently, research on EPA/DHA-PL mainly focuses on special and rare seafood, such as phospholipids derived from krill, sea cucumber, squid, and fish roe. However, it has been recently demonstrated that abundant EPA/DHA-PL can also be found in bulk fish and its by-products. Nonetheless, there is still limited research on the biological activities of EPA/DHA-PL derived from these sources. The aim of this study was to investigate the effect of phospholipid extracts from the heads of salmon and silver carp (S-PLE and SC-PLE) on the high-fat-diet-induced MetS in C57/BL mice. After an 8-week intervention, both SC-PLE and S-PLE had a significant ameliorating effect on MetS. Moreover, SC-PLE was more effective than S-PLE in reducing liver inflammation and fasting glucose. Both of the PL extracts were able to regulate the expression of key genes in lipid synthesis, fatty acid β-oxidation, and insulin signaling pathways. Compared with S-PLE, dietary SC-PLE had a greater influence on liver metabolomics. Pathway enrichment analysis showed that the differential metabolites of SC-PLE were mainly involved in arachidonic acid metabolism and glutathione metabolism. The results indicated that the different metabolic regulation methods of S-PLE and SC-PLE could be related to their variant molecular composition in EPA/DHA-PL.
期刊：
Food Research International,2022年161:111811 ISSN：0963-9969
通讯作者：
He, Jingren(jingren.he@whpu.edu.cn)
作者机构：
[Jiang, Tian; He, Jingren] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[Ye, Shuxin] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Liao, Wei] Univ Lyon, Univ Claude Bernard Lyon 1, CNRS, LAGEPP UMR 5007, 43 Blvd 11 Novembre 1918, F-69100 Villeurbanne, France.;[Wu, Muci; He, Jingren] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Natl R&D Ctr Se Rich Agr Prod Proc, Wuhan 430023, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE,LAQV, Rua Campo Alegre S-N, P-4169007 Porto, Portugal.
通讯机构：
[Jingren He] S;[Hélder Oliveira] L;LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal<&wdkj&>School of Food Science and Engineering, Wuhan Polytechnic University, 430023 Wuhan, China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
期刊：
International Journal of Biological Macromolecules,2022年213:718-727 ISSN：0141-8130
通讯作者：
Jie Cai
作者机构：
[Zhou, Rui; Wei, Ling-Feng; Cai, Jie; Chen, Lei; Zhang, Die; Yan, Xiao-Qin; Zhu, Wei-Jia; Ding, Wen-Ping] Wuhan Polytech Univ, Minist Educ, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Ma, Tie-Zheng] Beijing Technol & Business Univ, Beijing Engn & Technol Res Ctr Food Addit, Beijing 100048, Peoples R China.;[Wei, Ling-Feng; Cai, Jie; Zhang, Die; Zhu, Wei-Jia] Wuhan Polytech Univ, Natl R&D Ctr Serich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Pro, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Univ Swabi, Dept Agr, Anbar 23561, Khyber Pakhtunk, Pakistan.
通讯机构：
[Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, PR China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China
关键词：
Anthocyanin;Microgel;Starch
摘要：
We report a self-assembly method for the fabrication of multilayer-starch-based microgels used for anthocyanin encapsulation. Alcohol-heating treatment and ionization reactions were employed to reduce the crystallinity of starch and introduce ionic groups on the molecule to further cross-link it with sodium trimetaphosphate and produce a starch microgel. The molecular interactions between the starch and the anthocyanins facilitated the anthocyanin encapsulation and the starch-microgel/anthocyanin complexes with one, two, and three self-assembled layers were obtained. The Lay-1 microgel exhibited an encapsulation efficiency of 50.1% when the anthocyanin concentration, cross-linking starch concentration, contact time, and temperature were 0.25mg/mL, 1.5mg/mL, 3h, and 40°C, respectively. An increase in the number of layers resulted in a more compact microgel structure with the zeta potential presenting variations upon structural changes. Furthermore, the encapsulated anthocyanins presented a slow release from Lay-1, while the multilayered microgels (Lay-2 and Lay-3) displayed outstanding encapsulation stability. This study gives an insight on the encapsulation and release of anthocyanins by starch microgels, and provides a novel strategy for the design of starch-based encapsulation materials presenting great stability.
