通讯机构：
[Jiahua Wang] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Wuhan, 430023, People's Republic of China
摘要：
There is an urgent need to measure aflatoxin B(1) (AFB(1)) in food to prevent contaminated food consumption. In this work, a novel colorimetric enzyme-linked immunosorbent assay (ELISA) was developed for the detection of AFB(1) using curcumin as a colorimetric indicator. An indirect competitive enzyme-label immunoassay was developed using urease and rabbit anti-mouse immunoglobulin G labeled with gold nanoparticles as the signal-transduction tag. Urease catalyzed the hydrolysis of urea to produce ammonia, which increased the pH of the solution. The phenolic hydroxyl group of curcumin ionized into phenolic oxygen anions under alkaline conditions, which strengthened the synergistic effect of electron supply and absorption in curcumin. As a result, the color of curcumin changed from yellow to reddish-brown, producing a visible color change. Under optimal conditions, AFB(1) could be qualitatively determined with the naked eye, and quantitatively assessed by measuring the ratio of absorbance at wavelengths of 550 and 428nm. The change in the ratio of absorbance Δ(550)/Δ(428) decreased linearly in a range of 0.01-5ngmL(-1), and the limit of detection was 67pgmL(-1). Therefore, the selectivity and reliability of this proposed method were well validated. This method was also successfully used for the quantitation of AFB(1) in spiked rice flour and wheat flour samples. This approach may broaden the application field of colorimetric ELISA for aflatoxin, providing a promising platform for the rapid screening of aflatoxin in food.
摘要：
A rapid colorimetric method for detecting sodium benzoate in food products was established based on the d-amino acid oxidase (DAAO) and 2D metal organic framework (2D MOF) nanosheets mediated cascade enzyme reactions. Firstly, the synthesized 2D MOF nanosheets served as high efficient nanozyme with outstanding peroxidase-like catalytic activity and catalyzed the color reaction between H(2)O(2) and 3, 3', 5, 5'- tetramethylbenzidine. Secondly, sodium benzoate as a competitive inhibitor of DAAO, could influence the production of H(2)O(2) in DAAO mediated oxidation reaction. After a combination of those two reactions, this colorimetric quantitative method was constructed and validated for sodium benzoate determination with wide linear range (2.0-200.0μM), low limit of detection (2.0μM), high accuracy (recovery rate in 95.80-108.00%) and satisfied selectivity. Lastly, this method was utilized to analyze sodium benzoate concentration in juice, wine and vinegar by naked eyes.
通讯机构：
[E. Liao] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China<&wdkj&>Ministry of Education and Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, China<&wdkj&>National R&d Center for Se-richAgricultural Products Processing Technology, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Xu, Y.] S;Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Products, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China
摘要：
Colistin has broad-spectrum activity against Gram-negative bacteria and has been considered as the last-resort treatment for multiantibiotic-resistant Gram-negative bacteria infections in human. And it is also world widely utilized as a veterinary medicine for the promotion of growth, prevention and control of diseases in livestock and poultry. Extensive use of colistin in husbandry results in the introduction of large amounts of colistin to the surrounding environment via animals' urine and feces, potentially inducing the prevalence of colistin resistance bacteria and the impact of the ecological environment. The study investigated the adsorption, desorption and degradation of colistin in soils using high sensitivity UPLC-MS/MS assays. An MS based assay was established to directly determine colistin in the soil. It was observed that the moderate adsorption affinity of colistin to the three soils with adsorption strength (1/n) ranging from 0.6897 to 1.3333. Colistin exhibited the highest adsorption affinity to the sandy loam, followed by the sand and loam. Despite of different characteristics of three soils, the adsorption capacity of the three soils was comparable. The adsorption of colistin to the three types of soils analyzed was irreversible. The degradation experiments showed that the degradation of colistin in the sandy loam was relatively slow with a degradation half-life in a range of 13.2-29.7days when colistin was applied to the sandy loam at a level of 10 ~ 40µg/g. The degradation of colistin occurred in the mixture of the sandy loam and feces recovered from the colistin treated broiler as well. 25% of colistin remained in the mixture under environmental conditions after 14 days. Composting the sandy loam by directly covering the soil surface with colistin treated broilers' feces resulted in the introduction of colistin to the sandy loam. Colistin was observed in both the topsoil from the contact surface and sandy loam samples collected 20cm below the contact surface. The understanding of adsorption-desorption behaviors, degradation and mobility of colistin in soils might offer insights into the potential impact of colistin on the emergence and prevalence of resistant bacteria and the ecological environment.
通讯机构：
[Yang Yi] C;[Fei Huang] S;Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China<&wdkj&>College of Food Science and Engineering & Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Zizyphus jujube polysaccharide was extracted with hot water, ultrahigh pressure, deep eutectic solvent (DES) and ultrahigh pressure-assisted DES. Comparative analyses were conducted on the yield, physicochemical properties and prebiotic activity of four polysaccharides (JP-H, JP-U, JP-D and JP-UD). The yield of JP-UD (10.42 %) was 3.3 times that of JP-H (3.12 %), and its sugar content was the highest. JP-UD possessed the lowest Mw, while JP-H possessed the highest. Four JPs were acidic pyranose and mainly composed of galacturonic acid, arabinose and galactose. NMR results demonstrated that they contained not only similar glycosidic linkage but also the specific glycosidic linkage of -> 4)-alpha-D-Glcp-(l -> appeared in JP-U and JP-UD, the esterified units of GalA and CONH2 group appeared in JP-D and JP-UD, and the Terminal beta-D-Galp and -> 4)-alpha-GalpA-(1 -> appeared in JP-UD. JPs showed different proliferation effects on four lactobacillus strains, among which JP-UD exhibited the strongest prebiotic activity. Zizyphus jujube polysaccharides have great potential for application in the functional food and medical industry.
作者机构：
[Jia, Jilai; He, Jiangling; Zhou, Jiaojiao; Cai, Jie; Lv, Xuqin; Cai, Shiqi; Xie, Fang] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Lv, Xuqin] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Din, Zia-ud] Univ Swabi, Dept Agr, Swabi 23561, Pakistan.
通讯机构：
[Jie Cai] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Developing robust and sensitive food safety detection methods is important for human health. Electrochemiluminescence (ECL) is a powerful analytical technique for complete separation of input source (electricity) and output signal (light), thereby significantly reducing background ECL signal. ECL biosensors have attracted considerable attention owing to their high sensitivity and wide dynamic range in food safety detection. In this review, we introduce the principles of ECL biosensors and common ECL luminophores, as well as the latest applications of ECL biosensors in food analysis. Further, novel nanomaterial assembly strategies have been progressively incorporated into the design of ECL biosensors, and by demonstrating some representative works, we summarize the development status of ECL biosensors in detection of mycotoxins, heavy metal ions, antibiotics, pesticide residues, foodborne pathogens, and other illegal additives. Finally, the current challenges faced by ECL biosensors are outlined and the future directions for advancing ECL research are presented.
作者机构：
[An, Xiaoyu; Guo, Ziqi; Zhang, Rui; Wu, Muci; Tao, Wen; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pr, Sch Modern Ind Selenium Sci & Engn, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.;[Zhang, Rui; He, Jingren; Yang, Ning] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.;[Oliveira, Helder] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE, Rua Campo Alegre S-N, P-4169007 Porto, Portugal.
通讯机构：
[Rui Zhang; Jingren He] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, No. 36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, P. R. China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, No. 36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, P. R. China
作者机构：
[Zhang, Rui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Hubei Engn Res Ctr Deep Proc Green Serich Agr Pro, Natl R&D Ctr Serich Agr Prod Proc, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.;[Zhang, Qian; Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Zhang, Qian] Xiangyang Acad Agr Sci, Xiangyang 441004, Hubei, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Ciencias, REQUIMTE, LAQV,Dept Quim & Bioquim, Rua Campo Alegre S-N, P-4169007 Porto, Portugal.;[Jiang, Sijia; Ye, Shuxin] Yun Hong Grp Co Ltd, Nantong 430206, Hubei, Peoples R China.
通讯机构：
[Jingren He; Muci Wu] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, No. 36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, P. R. China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, P. R. China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, No. 36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, P. R. China
通讯机构：
[Zhou, Q.] O;Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Key Laboratory of Oil Seed Processing of Ministry of Agriculture, Oil Crops and Lipids Process Technology National and Local Joint Engineering Laboratory, Wuhan, China
期刊：
Ecotoxicology and Environmental Safety,2022年238:113568 ISSN：0147-6513
通讯作者：
Kui Zhu
作者机构：
[Huang, Xiaoyong; Zhu, Kui; Shao, Bing; Zhao, Xiaole; Peng, Wenjing] China Agr Univ, Coll Vet Med, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China.;[Zhao, Xiaole] Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Peoples R China.;[Huang, Xiaoyong; Zhang, Xin; Shao, Bing] Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Traceabil Technol Food, Beijing 100013, Peoples R China.;[Zhu, Kui] 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China.;[Shao, Bing] 16 Hepingli Middle St, Beijing 100013, Peoples R China.
通讯机构：
[Kui Zhu] C;College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing 100193, People’s Republic of China
通讯机构：
[Gang Liu] P;[Wenjing Huang; Hao Zhao] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China<&wdkj&>CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing, China<&wdkj&>Pharmacy Department, Renmin Hospital of Wuhan University, Wuhan, China
摘要：
By quenching the electronic excited state, self-aggregation of photosensitizers deteriorates the photodynamic therapy (PDT) outcome. Previously reported strategies to mitigate aggregation-caused-quenching (ACQ) involve harsh conditions and tedious synthesis processes. Moreover, failure to tune the extent of photosensitizer aggregation on-demand usually leads to a sub-optimal PDT effect. Herein, a new insight into ACQ alleviation by precisely tailoring the aggregation extent of photosensitizers via the confinement effect is unraveled by concise and facile coordination co-assembly fabrication of Pt/TCPP NCPs. Optimized meso-tetra(4-carboxyphenyl)porphine (TCPP) aggregation extent was achieved by precisely regulating the PES/TCPP feeding ratio to 12, unleashing outstanding PDT efficacy for robustly synergistic cancer PDT/chemotherapy.
通讯机构：
[Chao Chang; Jine Wu] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Key Laboratory for Processing and Transformation of Agricultural Products, Hubei, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
As a major component of extracellular matrixes (ECMs), collagen is an attractive biomaterial to fabricate porous scaffold for tissue engineering due to their similarity to the in vivo static microenvironment. However, the collagen-based porous scaffolds were difficult to mimic the dynamically remolded porous structure of ECM during the cell proliferation and tissue development, and always have poor mechanical property and not easy to handle. Here, natural collagen and partially denatured collagen was used to prepare the stepwise degradable hybrid bioscaffold with suitable mechanical property and dynamically remolded inner porous structure, which is desirable for the applications of tissue engineering. The collagen-based microporous scaffold was first prepared and used as physical support, then, the mechanical strength of which was reinforced by the import of the partially denatured collagen to give the hybrid bioscaffold. The fabrication conditions of the hybrid scaffolds were optimized, of which the thermal stability, mechanical property, and swelling property was explored. The stepwise enzymatic degradation process and the corresponding porous structure variation of the hybrid scaffold was confirmed by SEM and cell culture assays.
