A simple and rapid classification model for olive and Camellia oil was proposed based on ion mobility spectrometry (IMS) fingerprints and chemometric model (peak detection and random forest algorithm). Results indicated that IMS fingerprint spectra by second‐derivative algorithm could completely separate 64 olive oil and 79 Camellia oil samples used in this study by simply calculating the peak area. Random forest algorithm was employed to establish discriminant model for olive oil adulterated by Camellia oil. Simulated adulteration detection showed that the accuracy rate of discriminant model is 96.4% as two of 55 samples were identified as blending olive oil. All these results suggested that IMS could be an effective method to detect the adulterated olive oils by Camellia oil. Practical applications: Camellia oil is much similar to olive oil no matter in the physicochemical properties and fatty acid profiles. Thereby, olive oil has been one of the most frequent targets for the adulteration by Camellia oil. This study aimed to provide a rapid method to detect and separate olive oil and Camellia oil by a portable IMS device, by using fingerprints spectra, peak detection (first‐ and second‐derivative algorithm), and random forest algorithm. Results indicated that the classification and discriminant model established in this work was doable for the adulteration detection in the industry. Based on fingerprints spectra, peak detection (first‐ and second‐derivative algorithm) and random forest algorithm, IMS detection is doable for the rapid detection and separation of olive and Camellia oil.
Ye, Zhan;Qiao, Xue;Luo, Zhi;Hu, Chuanrong;Liu, Lingyi;He, Dongping
CyTA - Journal of Food,2016年14(4):604-612 ISSN：1947-6337
[He, Dongping; Luo, Zhi; Hu, Chuanrong; Ye, Zhan; Liu, Lingyi; Qiao, Xue] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China;[He, Dongping; Hu, Chuanrong; Liu, Lingyi; Luo, Zhi] Grain and oil resources comprehensive exploitation and engineering technology research center of State Administration of Grain, Wuhan, Hubei, 430023, China
[He, Dongping] Wuhan Polytech Univ, Sch Food Sci & Engn, Kejiao Bldg Room 801,Changqing Campus, Wuhan 430023, Hubei, Peoples R China.;School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, PR China;Grain and oil resources comprehensive exploitation and engineering technology research center of State Administration of Grain, Wuhan, Hubei 430023, China
Phospholipase C degumming;Water degumming;Process optimization;Degumming effect analysis and comparison;Desgomado con fosfolipasa C;Desgomado con agua;Optimización del proceso;Análisis del efecto del desgomado y comparación
ABSTRACT In the present study, the phospholipase C (PLC) degumming and water degumming process were studied, respectively, and each was optimized by orthogonal array experimental design; the minimum phosphorus content of PLC degumming was 7.34 ± 0.39 mg/kg, while that of water degumming was 61.54 ± 1.57 mg/kg. Oxidation stability of different oils were analyzed by testing the induction time, and the induction time of degummed oils were a little shorter than crude oil due to the removal of natural phospholipids which acted as antioxidants. Diacylglycerol and triglyceride analysis results showed that PLC degumming was superior to water degumming in increasing the oil yield and decreasing the neutral oil loss during the degumming process. According to the phospholipids composition analysis of degummed oil gums, it could be concluded that PLC was effective to remove the phosphatidylcholine and phosphatidylethanolamine, while having no activity on phosphatidylinositol or phosphatidic acid.
α‐Tocopherol, γ‐tocopherol and δ‐tocopherol were successfully separated and purified from soybean vitamin E concentrate (53% purity, from soybean oil deodorizer distillate) using a low‐pressure glass column (500 mm × 25 mm, I.D., packed with silica gel). The effects of eluent, flow‐rate and sample loading on separation efficiency were investigated using product purity and recovery as evaluation indices. On the basis of the single factor experiment results, an orthogonal test was designed to optimize the chromatographic separation conditions. The optimum conditions obtained by using analyses of extreme difference and variance are as follows: cyclohexane‐ethanol 99.7:0.3 (v/v), flow‐rate at 25 ml/min and loading amount being 2 ml (concentration 1 g/ml). Under these optimum conditions, purity of the α‐tocopherol, γ‐tocopherol and δ‐tocopherol were 92.35, 91.23, 89.95%, respectively; the recovery of those products were 35.21, 36.25, 61.25%, respectively. The advantage of this process is that high purity individual tocopherols can be obtained directly from soybean vitamin E concentrate at 53% purity, without additional purification steps.