作者： Li, Ping;He, Lang;Liu, Xueling;Fan, Shengxuan;Yuan, Yifan;...

期刊： BIOMEDICAL MATERIALS,2021年16(3):035019 ISSN：1748-6041

通讯作者： Wang, Haibo

作者机构： [Li, Ping] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Yuan, Yifan; Liu, Xueling; Li, Sheng; Fan, Shengxuan; Wang, Haibo; He, Lang; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： Biological characterization;Chitosan;Collagen;Electro-deposition;Tube-like hydrogel

摘要： Electro-deposition is a smart, safe and efficient method for biomaterial manufacturing. Collagen, a functional protein with excellent biocompatibility and biosafety, is a promising candidate for tissue engineering and biomedical applications. However, there are few reports on electro-deposition of biomaterials using collagen without electrically or magnetically active nanoparticles. In this study, electro-deposition was employed to swiftly fabricate tube-like collagen–chitosan hydrogels in a mild environment. Fourier transform infrared spectroscopy was employed to analyze the ingredients of the tube-like hydrogels. The result showed that the hydrogels contained both collagen and chitosan. The distribution and content of collagen in the hydrogels was further measured by hematoxylin–eosin staining and hydroxyproline titration. Collagen was distributed homogeneously and its content was related to the initial collagen:chitosan ratio. The tension resistance of the composite gels and the thermal stability of collagen in the composites were obviously enhanced by the chitosan doping. Meanwhile, the tube-like hydrogels retained a good ability to promote cell proliferation of collagen. This method offers a convenient approach to the design and fabrication of collagen-based materials, which could effectively retain the bioactivity and biosafety of collagen and furnish a new way to enhance the stability of collagen and the tensile strength of collagen-based materials. © 2021 IOP Publishing Ltd

语种： 英文

作者： 曹琴;舒飞翼;汪海波;未本美;李盛;...

期刊： 武汉轻工大学学报,2021年40(01):54-60 ISSN：2095-7386

作者机构： [曹琴] 武汉轻工大学食品科学与工程学院;[汪海波; 李盛; 许承志; 舒飞翼; 未本美] 武汉轻工大学化学与环境工程学院

关键词： 胶原;湿法纺丝;紫外辐照;热稳定性;耐酶降解;抗拉伸

摘要： 为提高胶原基生物材料的热稳定性,同时保持胶原自身良好的生物相容性,首先以安全无毒的磷酸盐缓冲液（PBS）为固定浴,通过湿法纺丝制备胶原纤维丝材料,以增强热稳定性和抗拉伸性能为目标,优化了制备条件。在此基础上,进一步通过紫外辐照,提高了胶原纤维丝的热稳定性和耐酶降解性。最后,通过体外酶降解实验和细胞毒性实验探索了胶原纤维丝的耐酶降解性能和促细胞增殖性能。研究结果表明湿法纺丝制备的胶原纤维丝具备自组装性能,最佳制备条件为20 mg/mL的胶原溶液和30×PBS的固定浴。紫外辐照能够提高胶原纤维丝的热稳定性并增强其抗拉伸强度和耐酶降解性能,但30 min以上的紫外辐照会大幅降低其抗拉伸强度。紫外辐照后的胶原纤维丝仍具有良好的促细胞增殖能力,可为胶原材料的性能提升提供一种新途径。

语种： 中文

作者： Zhang, Juntao;Deng, Fen;Liu, Wei;Huang, Yaozhi;Tu, Xiao;...

期刊： NEW JOURNAL OF CHEMISTRY,2020年44(48):21261-21270 ISSN：1144-0546

通讯作者： Wang, Haibo

作者机构： [Deng, Fen; Xu, Chengzhi; Wei, Benmei; Wang, Haibo; Tu, Xiao; He, Lang; Huang, Yaozhi; Kou, Huizhi; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Liu, Wei] Wuhan Polytecn Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Biocompatibility;Grafting (chemical);Hybrid materials;Attractive strategies;Collagen surface;In-situ polymerization;Modification conditions;Native functions;Polymer conjugate;Temperature-responsive;Thermosensitivities;Collagen;collagen;polymer;animal cell;Article;biocompatibility;collagen fibril;conjugate;controlled study;heat sensitivity;hybrid;NIH 3T3 cell line;nonhuman;polymerization;priority journal;surface property;temperature dependence

摘要： Collagen-polymer conjugates are being developed rapidly based on advancements in both polymer and protein science. The attachment of polymers to proteins can be achieved with the "grafting to" method or the "grafting from" method; the latter is more attractive. Although polymer-based collagen engineering provides an attractive strategy to regulate the native functions of collagen, there are still two important unresolved issues: the harsh modification conditions and the loss of fibrillogenesis property. Herein, a collagen-PNIPAAm conjugate (PCol) was prepared by the "grafting from" method to avoid harsh conditions. The amino groups of collagen were modified by a water-soluble NHS-functionalized ATRP initiator before in situ polymerization of NIPAAm on the collagen surface. On the other hand, the fibrillogenesis of PCol was realized by the induction of natural collagen, inspired by the xenogeneic collagen hybrid fibrils in organisms. Furthermore, the structure, fibrillogenesis, thermosensitivity, and biocompatibility of PCol were explored. This work may be useful in synthesizing optimized formulate collagen-polymer conjugates and helpful for the design of the novel collagen-based hybrid materials.

语种： 英文

作者： He, Lang;Yang, Jian;Xu, Chengzhi;Li, Sheng;Wei, Benmei;...

期刊： NEW JOURNAL OF CHEMISTRY,2020年44(17):6760-6770 ISSN：1144-0546

通讯作者： Wang, Haibo

作者机构： [Xu, Yuling; Xu, Chengzhi; Li, Sheng; Wei, Benmei; Wang, Haibo; He, Lang; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.;[Yang, Jian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430000, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

关键词： Collagen;Dichroism;Electrophoresis;High resolution transmission electron microscopy;Mammals;Molecular structure;Pore size;Scanning electron microscopy;Shear deformation;Shearing;Sodium dodecyl sulfate;Sulfur compounds;Viscosity;Assembly behaviors;Bovine tendon collagens;Characteristic peaks;Collagen molecules;Equilibrium moduli;Physical modification of proteins;Sodium dodecyl sulfate-polyacrylamide gel electrophoresis;Weak intermolecular interactions;Shearing machines;collagen gel;Article;bovine;chemical structure;circular dichroism;collagen degradation;collagen fiber;controlled study;flow kinetics;investigative procedures;molecular weight;nonhuman;polyacrylamide gel electrophoresis;pore size;porosity;priority journal;scanning electron microscopy;shear rate;shear treatment;time;transmission electron microscopy;triple helix

摘要： Shear treatment is always employed in the extraction, preparation and research of collagen materials. This study is aimed at investigating the effect of the pre-shearing treatment of neutral bovine tendon collagen solution on the molecular structure, assembly behavior and gel properties of collagen. Rheological and assembly degree (AD) tests showed that with the increase in the shear rate or the extension of shear time during the pre-shearing treatment, the equilibrium moduli and AD of collagen gels that assemble from the pretreated samples gradually decreased. Scanning electron microscopy (SEM) results indicated that with the deepening of the pre-shearing treatment (increased shear rate or shear time), the diameter of the resultant collagen fibers gradually decreased, and their pore size and porosity increased as a whole. The viscosity test of the collagen solution obtained after pre-shearing revealed that the viscosities of the samples were lower than that of the sample devoid of pre-shearing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism (CD) analysis manifested that the molecular weights and CD characteristic peaks of the collagen molecules remained unchanged in the sheared solution. However, with the deepening of the pre-shearing treatment, the proportion of α chains in the SDS-PAGE image increased, while the total content of β and γ chains decreased; the triple-helix structure of collagen in the CD spectrum decreased, and the random coiled structure increased. Moreover, transmission electron microscopy (TEM) displayed that the D-periods of the fibers assembled from the sheared samples were also decreased. A mechanism for the effect of the pre-shearing treatment of the collagen solution on the molecular structure, assembly behavior and gel properties of collagen has been proposed. The findings in this work may provide new understanding and theory on the physical modification of proteins and the construction of new materials from polymer systems that are aggregated or assembled based on weak intermolecular interactions. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2020.

语种： 英文

作者： He, Lang;Li, Sheng;Xu, Chengzhi;Wei, Benmei;Zhang, Juntao;...

期刊： Macromolecular Research,2020年28(9):861-868 ISSN：1598-5032

通讯作者： Wang, Haibo

作者机构： [Xu, Yuling; Xu, Chengzhi; Li, Sheng; Zhu, Beirong; Wu, Xilin; Wei, Benmei; Cao, Yang; Wang, Haibo; He, Lang; Huang, Rongrui; Xiong, Zhijin; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.;[Yang, Jian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430000, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

关键词： Amino acids;Biocompatibility;Collagen;Composite materials;Crosslinking;Degradation;Elastic moduli;Electrophoresis;Hydrogels;Mammals;Sodium dodecyl sulfate;Sulfur compounds;Swelling;Temperature;Tendons;Cold waters;Collagen composites;Composite hydrogels;Composites material;Gel properties;Gelatin-collagen composite hydrogel;Lows-temperatures;Self-healing properties;Sol'gel;Sol-gel property;Sol-gels

摘要： Pure collagen materials are expensive with poor mechanical properties, which need modifications in most cases. As the degradation product of collagen, gelatin is cheap, degradable and biocompatible, but few literatures have reported the research about gelatin-collagen composite materials. This is because gelatin and collagen have different soluble temperatures—gelatin is soluble in hot water (≥30 °C) and swells in cold water. However, a low temperature (2–10 °C) is required to prepare and store collagen solution, and neutral collagen solution denatures quickly above the room temperature. In this study, gelatin was ground into powders and swelled in neutral bovine tendon pepsin-soluble collagen solution (BPSC) to form a homogeneous gelatin-collagen mixture, in light of the swelling characteristics of gelatin in cold water. The assembly properties and gel properties of this composite material were further studied. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) test results showed that the bovine tendon collagen had typical type-I collagen structural characterizations with two α chains of about 100 kDa and one β chain of about 200 kDa; while the SDS-PAGE pattern of gelatin displayed bands continuously distributed from 30 to 200 kDa. Amino acid composition analysis test indicated that the content of polar amino acids and the sum of acidic and base amino acids for gelatin were higher than that of BPSC. Studies on gel properties demonstrated that gelatin-collagen mixed solution had collagen-like assembly characteristics and assembly kinetics. The moduli of the assembled gel at 35 °C were equivalent to that of pure bovine tendon collagen system; moreover, the system moduli didn’t change with time with elastic moduli (G′) of about 40 Pa. However, at 25 °C, the moduli of gelatin-collagen composite hydrogel increased with the extension of time, its G′ increased about 18 times within 8 h, and the ratio of elastic modulus to viscous modulus (G″) increased 4.6 times, showing a significant aging effect of structural strength. Meanwhile, the mechanical strength of the composite hydrogel was also regulated by temperature—the gel was highly elastic (G′≈3,000 Pa, G′>>G″) at a low temperature (5 °C); as the temperature rose, the system moduli gradually decreased and the elastic gel transformed into waterlike fluid at 50 °C little by little. What’s more, gelatin-collagen composite hydrogel also had reversible sol-gel performances and self-healing capability similar to the gelatin hydrogel. This novel preparation method for preparing composite materials and the resultant composite hydrogel are expected to be used in the fields of natural food gels, injectable hydrogels, cell scaffolds, drug sustained-release materials and so on, and improve and promote the processing performances, price and large-scale production of collagen-based materials.[Figure not available: see fulltext.]. © 2020, The Polymer Society of Korea and Springer.

语种： 英文

作者： Cai, Jie*;Zhang, Die;Ding, Wen-Ping*;Zhu, Zhen-Zhou;Wang, Guo-Zhen;...

期刊： ACS OMEGA,2020年5(46):29896-29902 ISSN：2470-1343

通讯作者： Cai, Jie;Ding, Wen-Ping;Fei, Peng

作者机构： [Cai, Jie; Zhu, Zhen-Zhou; Wang, Guo-Zhen; Zhang, Die; Ding, Wen-Ping; He, Jing-Ren] Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ,Hubei Key Lab Proc & Transformat Agr, Wuhan 430023, Peoples R China.;[Wang, Hai-Bo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Si, Tian-Lei; Cai, Jie] Henan Univ Technol, Prov Key Lab Cereal Resource Transformat & Utiliz, Zhengzhou 450001, Henan, Peoples R China.;[Fei, Peng] Minnan Normal Univ, Sch Biol Sci & Biotechnol, Zhangzhou 363000, Peoples R China.

通讯机构： [Cai, J; Ding, WP] W;[Cai, Jie] H;[Fei, Peng] M;Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ,Hubei Key Lab Proc & Transformat Agr, Wuhan 430023, Peoples R China.;Henan Univ Technol, Prov Key Lab Cereal Resource Transformat & Utiliz, Zhengzhou 450001, Henan, Peoples R China.

摘要： Improving the electrochemical performance of biomass-derived carbon electrode-active materials for supercapacitor applications has recently attracted considerable attention. Herein, we develop hybrid electrode materials from rice-husk-derived porous carbon (RH-C) materials and βNi(OH)2 via a facile solid-state reaction strategy comprising two steps. The prepared RH-C/Ni(OH)2 (C−Ni) was investigated using scanning electron microscopy (SEM) (energy-dispersive X-ray spectrometer (EDS)), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) to acquire the physical and chemical information, which was used to demonstrate the successful fabrication of C−Ni. Thermogravimetric analysis (TGA) measurement results confirmed that the thermal stability of C−Ni changed due to the presence of Ni(OH)2. As expected, C−Ni possesses a high capacitance of ∼952 F/g at a current density of 1.0 A/g. This result is higher than that of pure biomass-based carbon materials under the three-electrode system. This facile preparation method, which was used to synthesize the electrode-active materials, can extend to the value-added utility of other waste biomass materials as high-performing supercapacitor electrodes for energy storage applications. © 2020 American Chemical Society.

语种： 英文

作者： Xu, Chengzhi;Wei, Xu;Shu, Feiyi;Li, Xinxin;Wang, Wenxin;...

期刊： International Journal of Biological Macromolecules,2020年153:232-239 ISSN：0141-8130

通讯作者： Wang, Haibo

作者机构： [Xu, Chengzhi; Li, Xinxin; Li, Yuanyuan; Wang, Wenxin; Wang, Haibo; Shu, Feiyi; Wei, Xu; Li, Siman; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Li, Ping] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Collagen;Induction method;Multi-layered network;Self-assembly;Ultraviolet irradiation

摘要： Ultraviolet (UV) irradiation is a common method of molecular crosslinking and sterilization of collagen. In this study, UV irradiation at low temperature conditions was investigated as an induction and regulation method for fiber-like aggregation and gelation of collagen. Differences between gelation processes induced by UV irradiation and by traditional temperature-induced methods as well as differences in the properties of the gelation products were systematically analyzed. We found that UV irradiation can induce fiber-like aggregation and gelation of bovine tendon collagen at lower temperatures (<17 degrees C) than the usual temperature of 37 C. During UV irradiation, cross-linking and degradation of collagen molecules occurred along with typical collagen fiber formation. The collagen fibers, together with the grafted collagen molecules and the degraded collagen peptides, formed a gel product that had a unique, multi-layered network structure. Collagen gels induced by UV irradiation at low temperatures displayed improved thermal stability, mechanical strength, and cell-growth promoting ability compared with collagen gels that were induced at 37 degrees C. Our results open up new avenues for the production of collagen-based biomaterials. (C) 2020 Published by Elsevier B.V.

语种： 英文

作者： Sun, Man;Wei, Xu;Wang, Haibo*;Xu, Chengzhi*;Wei, Benmei;...

期刊： Food and Bioprocess Technology,2020年13(2):367-378 ISSN：1935-5130

通讯作者： Wang, Haibo;Xu, Chengzhi

作者机构： [Sun, Man] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Li, Sheng; Wang, Haibo; He, Lang; Xu, CZ; Wei, Xu; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, HB; Xu, CZ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Collagen;Structure restoration;Thermal denaturation;Ultrahigh pressure

摘要： Thermal denaturation is the primary reason for the loss of excellent performance for collagen-based materials. It is particularly urgent to explore an effective structure restoration strategy for thermally denatured collagen. In this study, three different degrees of collagen thermal denaturation models were constructed; the effects of ultrahigh pressure treatments of varying intensities on the structure and properties of partially thermal denatured collagen were investigated. Those results showed that ultrahigh pressure partially restores the triple helix conformation and the α helix of low thermally denatured collagen molecules and moderately restores collagen thermal stability. However, this recovery is not a 'perfect' structural restoration; it cannot effectively restore the fibrotic ability of collagen which leads to a decrease in the fiber diameter of the product and an increase in the storage modulus of the product gel. As the degree of thermal denaturation of collagen increases, the ultrahigh pressure recovery effect is gradually lost. For moderate thermally denatured collagen, ultrahigh pressure treatments only recover a small amount of its triple helical conformation. For high thermally denatured collagen, ultrahigh pressure treatments exacerbate the destruction of the three-dimensional structure of collagen molecules, thereby losing the order of molecular structure and the capability of fibrosis self-assembly. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

语种： 英文

作者： Yang, Jian;Wang, Haibo*;He, Lang*;Wei, Benmei;Xu, Chengzhi;...

期刊： Macromolecular Research,2019年27(11):1124-1135 ISSN：1598-5032

通讯作者： Wang, Haibo;He, Lang

作者机构： [Yang, Jian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430000, Hubei, Peoples R China.;[Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Li, Sheng; Wang, Haibo; He, Lang; Zhang, Juntao; He, L] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

通讯机构： [Wang, HB; He, L] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

关键词： Cell proliferation;Differential scanning calorimetry;Gels;Hydrophilicity;Mammals;Morphology;Quenching;Tendons;Viscoelasticity;Bovine tendon collagens;Grass carp skin;reconstituted fibril;regulation;Viscoelastic properties;Collagen

摘要： Heterotypic collagen fibril has long been found in the tissues of organisms, which plays an important role in the formation and function of complex structures of organisms. Inspiring by the phenomenon, scholars tried to incubate collagens from different sources into novel collagen materials in vitro, and the forming of heterogenic reconstituted collagen fibrils (RF) was often demonstrated by differential scanning calorimetry (DSC) and fluorescence quenching analysis. In this work, we used two type-I collagens from different species (bovine tendon and grasscarp fish skin) to co-assemble in vitro, and verified the formation of RF from a new rheological perspective. In addition, we also investigated the assembly behavior, surface hydrophilicity and hydrophobicity, microscopic morphology and cell proliferation ability of the RF. The results showed that the assembly rate, surface properties, fibril size, viscoelastic properties of RF can be delicately regulated by the method of heterogenic collagen reconstitution. This study provides new experimental evidence for the reconstitution of heterogenic collagens, and also offers a new means for the regulation of collagen gel performance, which would help to expand the application range of collagen gel materials. [Figure not available: see fulltext.] © 2019, The Polymer Society of Korea and Springer.

语种： 英文

作者： 代纯;靳熙茜;汪海波;未本美;李盛;...

期刊： 武汉轻工大学学报,2019年38(01):9-16 ISSN：2095-7386

作者机构： 武汉轻工大学 食品科学与工程学院,湖北 武汉,430023;武汉轻工大学 化学与环境工程学院,湖北 武汉,430023;[汪海波; 靳熙茜; 许承志; 何浪; 李盛; 徐玉玲; 未本美; 张军涛; 代纯] 武汉轻工大学

关键词： 桂花;乙醇提取物;层析分离;抗氧化活性;初步结构鉴定

摘要： 桂花富含天然抗氧化成分,在天然抗氧化剂领域具有良好的开发应用潜力。本研究以制取香料浸膏后的桂花残渣为原料,以乙醇为溶剂提取桂花中的抗氧化物质,得到桂花乙醇粗提物,并对该粗提物开展溶解性能、pH稳定性和热稳定性等理化性质分析。随后我们运用硅胶柱层析技术,对该粗提物进行极性分级,并对不同分级产物开展体外抗氧化活性的比较分析。在此基础上,进一步对具有良好抗氧化活性的分级产物开展了初步的结构鉴定,结果表明:桂花乙醇萃取物在溶解度参数大于10的溶剂中有较大的溶解度;其在中性、酸性和温度低于60℃的条件下较为稳定;此乙醇粗提物经硅胶柱层析分离可以得到3个分级产物。体外抗氧化活性评价结果表明,分级产物中组分1(Frac1)具有良好的1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除和还原能力,而组分3(Frac3)抑制亚油酸氧化的能力最强。高效液相色谱-质谱联用分析结果表明,Frac1中活性成分主要为黄酮甙类物质,而Frac3中的主要活性组分为双黄酮类物质。

语种： 中文

作者： Shu, Feiyi;Dai, Chun;Wang, Haibo*;Xu, Chengzhi*;Wie, Benmei;...

期刊： ChemistrySelect,2019年4(45):13370-13379 ISSN：2365-6549

通讯作者： Wang, Haibo;Xu, Chengzhi

作者机构： [Xu, Yuling; Xu, Chengzhi; Li, Sheng; Wang, Haibo; Wie, Benmei; He, Lang; Shu, Feiyi; Xu, CZ; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Dai, Chun] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, HB; Xu, CZ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： amino acids;collagen;self-assembly;stability;triple helix conformation

摘要： <jats:title>Abstract</jats:title><jats:p>The unique sequence of the α‐chain is the structural cornerstone of the collagen triple helix that dictates its conformation and molecular behavior. In this study, collagen‐like peptides of varying sequence characteristics were designed to elucidate the role of Ser (polar residues), Ala (non‐polar residues), and Arg/Glu (ionizable residues) in the formation, kinetics, and stability of the triple helix, and its self‐assembly behavior. We found that the introduction of other common amino acids in (Gly‐Hyp‐Pro)<jats:sub>n</jats:sub> results in varying degrees of decrease in the rate of formation and stability of the triple helix conformation and the self‐assembly into higher order structures. The introduction of Ser showed a maximal detrimental effect on these processes, followed by the Arg/Glu and Ala. Collagen‐like peptides with Ser could not form thermodynamically stable triple helix conformations and lost their ability to further self‐assemble. Arg/Glu promoted the self‐assembly of collagen‐like peptides, and electrostatic interactions and hydrogen bonding were key to the self‐assembly of periodic ordered structures similar to natural collagen. The hydrophobic interactions of Ala also accelerated self‐assembly to some extent, however, due to the lack of directionality of hydrophobic interactions, the formation of the long‐range ordered structure was affected negatively.</jats:p>

语种： 英文

作者： Dai, Lei;Nan, Jie;Tu, Xiao;He, Lang;Wei, Benmei;...

期刊： Cellulose,2019年26(11):6713-6724 ISSN：0969-0239

通讯作者： Wang, Haibo;Zhang, Juntao

作者机构： [Dai, Lei] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Li, Sheng; Wang, Haibo; Tu, Xiao; He, Lang; Zhang, Juntao; Nan, Jie] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Wang, HB; Zhang, JT] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： Collagen;Bacterial cellulose;Fibrillogenesis;Thermostability;Cytocompatibility

摘要： Abstract: Composite bacterial cellulose (BC) membranes containing collagen fibrils or collagen monomers (named BC/Col-fibre or BC/Col, respectively) were fabricated by immersing macroporous BC in a 3.0mg/mL collagen solution for 50h at 4°C with or without collagen fibrillogenesis before lyophilization. The structure of the membranes was characterized by scanning electron microscope, nitrogen adsorption–desorption experiment, and X-ray diffraction. The structure became much more compact upon the introduction of collagen but the crystal structure of BC did not change. The thermal stability and the cytocompatibility of the membranes were evaluated by thermogravimetric analysis and a cell adhesion assay, respectively. The thermal stability of BC was enhanced by collagen incorporation and by fibrillogenesis. The adhesive ability and the proliferation of NIH/3 T3 fibroblast cells in BC/Col-fibre were better than that in BC/Col, which was still superior to that in BC. Graphic abstract: Bacterial cellulose/collagen composite membranes (BC/Col) with and without collagen fibrils were fabricated. The collagen amount was improved to 12% by immersing the macroporous BC in a 3.0mg/mL collagen solution for 50h at 4°C. The collagen fibrils interpenetrated with the BC nanofiber networks to improve the thermostability and the cytocompatibility of BC/Col.[Figure not available: see fulltext.]. © 2019, Springer Nature B.V.

语种： 英文

作者： Jiang, Fang-Cheng;Zhou, Rui;Cai, Jie*;Dong, Qi;Ding, Wen-Ping;...

期刊： BIORESOURCES,2019年14(1):1252-1264 ISSN：1930-2126

通讯作者： Cai, Jie;Liu, Gang;Wang, Guo-Zhen

作者机构： [Liu, Gang; Cai, Jie; Liu, G; Wang, GZ; Zhou, Rui; Chen, Xuan; Dong, Qi; Jiang, Fang-Cheng; Wang, Guo-Zhen; He, Jing-Ren; Ding, Wen-Ping] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Liu, Gang; Cai, Jie; Liu, G; Wang, GZ; Wang, Guo-Zhen; He, Jing-Ren; Ding, Wen-Ping] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan, Hubei, Peoples R China.;[Liu, Gang; Cai, Jie; Liu, G; Wang, GZ; Wang, Guo-Zhen; He, Jing-Ren; Ding, Wen-Ping] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Wang, Hai-Bo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Cai, J; Liu, G; Wang, GZ] W;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan, Hubei, Peoples R China.;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.

关键词： Adsorption;Complex enzymatic hydrolysis;Porous purple sweet potato starch

摘要： This study aimed to develop a facile synthesis process of porous starch wall materials from purple sweet potato (PSP) for microencapsulation via enzymatic treatment. The optimum extraction conditions of purple sweet potato starch (PSP-S) were first attained by an orthogonal experiment. Response surface methodology was performed through the enzymatic hydrolysis of PSP-S using an α-amylase and glucoamylase complex to optimize the process parameters for the production of porous starch. Optimal reaction conditions were: the mass ratio (w/w) of glucoamylase to α-amylase of 6.39, amount of substrate of 19.5 g, and amount of enzymes of 0.53%. The surface morphology, microstructure, and thermal stability of the obtained samples were characterized with scanning electron microscopy, Fourier-transform infrared, and thermogravimetric analyses, respectively. The purple sweet potato porous starch (PSP-PS) had a stable oil adsorption capacity, and the molecular structure and thermal stability of porous starch were not substantially different from those of native starch. This study offers a simple yet efficient approach to produce fully biodegradable food-based porous materials for potential applications in oil microencapsulation. © 2019 North Carolina State University.

语种： 英文

作者： Nan, Jie;Zou, Meilian;Wang, Haibo*;Xu, Chengzhi*;Zhang, Juntao;...

期刊： International Journal of Biological Macromolecules,2018年111:200-207 ISSN：0141-8130

通讯作者： Wang, Haibo;Xu, Chengzhi

作者机构： [Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Wang, Haibo; He, Lang; Xu, CZ; Nan, Jie; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Zou, Meilian] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, HB; Xu, CZ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

摘要： Ultra-high pressure technology has attracted a great deal of attention in recent years, and has been widely used in food science, medicine, and other fields. This study aimed to determine the effect of ultra-high pressure on the structure and properties of collagen. Native collagen extracted from bullfrog skin was processed under different ultra-high pressure treatment conditions (300, 400, and 500 MPa). Then systematic analysis of the molecular structures and properties of the samples after ultra-high pressure treatment were performed. It was found that the conformation of collagen molecules could be adjusted by ultra-high pressure treatment, and this regulation was closely related to the level of treatment pressure. A possible mechanism of the impact of ultra-high pressure on the collagen molecular structures was speculated according to the experimental results. At low pressure levels (300-400 MPa), the pressure perpendicular to collagen axis dominates and leads to a tightening of the triple helix structure of collagen, while the pressure parallel to collagen axis is dominant and the triple helix tends to dissociate like a zipper at high pressure levels (&gt; 400 MPa). These structural changes would simultaneously result in various changes to thermal stability, self-assembly properties, and antigenicity of collagen. (C) 2018 Elsevier B.V. All rights reserved.

语种： 英文

作者： Cai, Jie*;Zhou, Rui;Li, Tiantian;He, Jingren*;Wang, Guozhen;...

期刊： Cellulose,2018年25(1):391-398 ISSN：0969-0239

通讯作者： Cai, Jie;He, Jingren;Xiong, Hanguo

作者机构： [Zhou, Rui; Cai, Jie; He, Jingren; Li, Tiantian; Wang, Guozhen] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Cai, Jie; Xiong, Hanguo] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Wang, Haibo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Cai, J; He, JR] W;[Xiong, Hanguo] H;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.

关键词： Cellulose;Electrospinning;Nanofiber

摘要： Catalysts play a key role in the production of cellulose acetate (CA). In this study, CA derived from bamboo cellulose (BC) was synthesized in a solution mixture of acetic acid and acetic anhydride that contains catalysts. The catalytic activities of three catalysts (i.e., sulfuric, methyl benzene sulfonic, and dodecyl benzene sulfonic acids) were investigated in terms of kinetics of acetylation, which was established according to the first-order reaction mechanism. Based on this, cellulose-based nanofibers were further prepared from the acetylated BC solution using an electrospinning technique. Results in this study may be useful for the preparation and application of nanomaterials derived from natural polymeric materials. © 2017, Springer Science+Business Media B.V., part of Springer Nature.

语种： 英文

作者： 戴蕾;胡秋林;汪海波;徐玉玲;张军涛;...

期刊： 武汉轻工大学学报,2018年37(06):14-22 ISSN：2095-7386

作者机构： 武汉轻工大学食品科学与工程学院,湖北武汉,430023;武汉轻工大学化学与环境工程学院,湖北武汉,430023;[汪海波; 许承志; 戴蕾; 何浪; 李盛; 徐玉玲; 未本美; 张军涛; 胡秋林] 武汉轻工大学

关键词： 草鱼;胶原纤维;环境因子;调控;形貌;性能

摘要： 体外纤维重组是天然胶原的重要分子行为,直接影响胶原的性能和应用。本研究以草鱼胶原为研究对象,系统观察了体系温度、pH、离子强度和时间等环境因素对重组胶原纤维形貌和粒径分布的影响。在此基础上,进一步比较了不同环境因子调控下,胶原重组纤维产物的耐酶降解性能和热稳定性能。结果表明,重组胶原纤维粒径和粒径分布受环境因子的调控。中性pH环境下胶原纤维粒径大但分布不均一,体系温度越高、离子强度越大,重组纤维粒径越小,延长纤维重组时间有利于纤维粒径的增大。纤维产物性能分析结果表明,纤维粒径越大,则胶原重组纤维产物的耐酶降解性能和热稳定性能越好。

语种： 中文

作者： Xu, Chengzhi;Nan, Jie;Dai, Chun;Wei, Benmei;Zhang, Juntao;...

期刊： Drying Technology,2018年36(9):1031-1040 ISSN：0737-3937

通讯作者： Wang, Haibo

作者机构： [Xu, Chengzhi; Wei, Benmei; Wang, Haibo; He, Lang; Nan, Jie; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.;[Dai, Chun] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： Cell proliferation;collagen gel;drying methods;self-assembly

摘要： In this study, the effects of collagen self-assembly, drying methods, and reswelling on collagen gel structure and biological properties were determined. The results showed that the self-assembly of collagen improved the regularity and pore size of the collagen gel and promoted collagen gel thermostability and cell proliferation. After drying and reswelling, the main structure of the self-assembly gel was composed of lamellar gel walls, but the gel structure shrank to some extent and pore size was reduced. The structure of the self-assembled gel after drying and reswelling was closely related to that of the drying method. © 2017 Taylor & Francis.

语种： 英文

作者： Lei, Miao;Jiang, Fang-Cheng;Cai, Jie*;Hu, Si;Zhou, Rui;...

期刊： International Journal of Biological Macromolecules,2018年111:755-761 ISSN：0141-8130

通讯作者： Cai, Jie;He, Jing-Ren;Xiong, Xin-Gao

作者机构： [Liu, Gang; Zhou, Rui; Cai, Jie; Hu, Si; He, Jing-Ren; Wang, Yue-Hui; Jiang, Fang-Cheng; Lei, Miao] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Wang, Hai-Bo] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.;[Xiong, Xin-Gao] Huazhong Univ Sci & Technol, Dept Otorhinolaryngol Head & Neck Surgery, Union Hosp, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China.;[Liu, Gang; Cai, Jie; He, Jing-Ren; Wang, Yue-Hui] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan, Hubei, Peoples R China.;[Liu, Gang; Cai, Jie; He, Jing-Ren; Wang, Yue-Hui] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Cai, J; He, JR] W;[Xiong, Xin-Gao] H;Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Huazhong Univ Sci & Technol, Dept Otorhinolaryngol Head & Neck Surgery, Union Hosp, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan, Hubei, Peoples R China.

关键词： Starch;Porous starch;Microencapsulation

摘要： In this study, starch with porous structures derived from purple sweet potato was prepared and used as a food grade polymer for the microencapsulation of olive oil. The optimal reaction conditions for preparing porous starch were determined to improve its adsorption capacity as effective microcapsule-wall materials. Olive oil was then impregnated in microspheres, and loading ratio was optimized by investigating the restrictive factors, including the mass ratio of olive oil to porous starch, as well as the embedding temperature and time. The presence of olive oil in the starch matrix was confirmed by SEM, FTIR, and TGA. Results demonstrated that the porous starch-based microencapsulation exhibit a stable olive oil loading ratio and a significant improvement in oxidative stability compared with free olive oil. The newly-proposed process used in this work was easy to scale up for developing a new and attractive method for oil protection in the food industry. (C) 2018 Elsevier B.V. All rights reserved.

语种： 英文

作者： Wei, Benmei;Nan, Jie;Jiang, Ying;Wang, Haibo*;Zhang, Juntao;...

期刊： Food Biophysics,2017年12(4):422-432 ISSN：1557-1858

通讯作者： Wang, Haibo

作者机构： [Xu, Chengzhi; Zhai, Zhongwei; Xie, Dong; Wei, Benmei; Wang, Haibo; He, Lang; Xie, Shuchang; Nan, Jie; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Jiang, Ying] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Collagen;Self-assembly;Hybrid fibril;Physicochemical properties

摘要： A Hybrid collagen fibril (HCF) assembled from xenogeneic collagens is a special kind of collagen fibrils in vivo and plays an important role in living systems. Inspired by nature, can a HCF form in vitro? Herein, we fabricated a new HCF by neutralizing a mixture of type I bullfrog (Rana catesbeiana Shaw) skin collagen and porcine (Sus scrofa domesticus) skin collagen with a phosphate buffer, and investigated its physicochemical properties. Self-assembly kinetics and fluorescence-quenching experiments showed that a significant intermolecular interaction and co-assembly behavior occurred between bullfrog skin collagen and porcine skin collagen, thus confirming that xenogeneic collagens can self-assemble to form HCF. Differential scanning calorimetry revealed that the thermal stability of HCF was completely different from that of the syngeneic bullfrog skin and porcine skin collagen fibrils. This finding indicated that a new kind of collagen fibril was fabricated successfully. Scanning electron microscopy and transmission electron microscopy tests showed that the diameters and D-periodicity lengths of HCF were smaller than those of the syngeneic collagen fibrils, suggesting that the morphological features of HCF were distinguished from those of the syngeneic fibril samples. Moreover, viscoelasticity of a collagen gel also changed after the self-assembly of xenogeneic collagens. Meanwhile, the obtained hybrid gel still exhibited good biocompatibility and cell proliferation properties. Finding from this work provides a new idea for the improvement or regulation of collagen-based products performance.

语种： 英文

作者： 刘闪;刘良忠;陈阳明;杨永生;汪海波

期刊： 中国食品学报,2016年16(9):185-191 ISSN：1009-7848

通讯作者： Liu, Liangzhong(liu2022888@163.com)

作者机构： [刘闪; 刘良忠; 陈阳明; 杨永生; 汪海波] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan;430023, China;Aquatic Products Engineering and Technology Research Center of Wuhan City, Wuhan;[刘闪; 刘良忠; 陈阳明; 杨永生; 汪海波] 430023, China <&wdkj&> Aquatic Products Engineering and Technology Research Center of Wuhan City, Wuhan;[刘闪; 刘良忠; 陈阳明; 杨永生; 汪海波] 430023, China

通讯机构： College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China

关键词： 螯合钙;结构表征;增殖作用

摘要： 为开发一种具有良好吸收性和高利用率的补钙产品———胶原低聚肽螯合钙, 探究其结构特征及其对细胞的促生长作用。分别采用傅里叶红外光谱、紫外光谱、荧光光谱和差示热量扫描仪对胶原低聚肽螯合钙冻干样品进行表征分析,利用293Ad5+人肾细胞进行增殖培养试验,结果表明:胶原低聚肽中氨基上的氮原子与羧基上的氧原子均参与了钙离子的螯合反应,它们之间主要是配位结合。差示热量扫描(DSC)曲线表明:胶原低聚肽螯合钙具有比胶原低聚肽更好的稳定性。采用MTT法检测不同剂量组胶原低聚肽螯合钙对293Ad5+人肾细胞培养24h的相对增殖活力,结果表明胶原低聚肽螯合钙安全性高,在一定程度上促进了细胞的增殖。

语种： 中文