三乳酸甘油酯对冷应激肉鸡生产性能、抗氧化能力和能量代谢状况的影响
作者:
廖满;程强;李叶涵;李宝成;杨书慧;...
期刊:
中国家禽 ,2016年38(2):18-24 ISSN:1004-6364
作者机构:
动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院,湖北武汉430023;中国农业大学动物科技学院,北京,100193;[丁斌鹰; 杨书慧; 李宝成; 廖满; 程强; 易丹; 李叶涵] 武汉轻工大学;[杨鹰] 中国农业大学
关键词:
三乳酸甘油酯;冷应激;能量代谢;肉鸡
摘要:
试验旨在研究三乳酸甘油酯对冷应激肉鸡生产性能、抗氧化能力和能量代谢状况的影响.选用150只1日龄Cobb肉鸡,随机分为3个处理组:对照组、冷应激组、乳酸甘油酯组,分别饲喂基础日粮、基础日粮、基础日粮+500 mg/kg三乳酸甘油酯,每组5个重复,每个重复10只鸡.试验第1周各组温度控制在32~34℃,第2周控制在30~32℃,试验第3~6周冷应激组、乳酸甘油酯组控制在(10±2)℃,而对照组维持在(26±2)℃,湿度50%~60%.结果显示:①与对照组相比,冷应激处理降低了肉鸡生产性能,心脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px),肝脏SOD及肺脏SOD、GSH-Px的酶活力(P<0.05),同时也降低了心脏、肝脏三磷酸腺苷(ATP)含量,心脏腺苷酸池(TAN)含量、低氧诱导因子-1(HIF-1)、血红素加氧酶-1(HMOX-1)mRNA水平及肝脏HIF-1、过氧化物酶体增殖活化受体γ辅助活化因子1 α(PGC-1α)mRNA水平(P<0.05),但提高了心脏相对重量、肝脏AMP/ATP比值及心脏和肝脏AMPK-α1 mRNA水平(P<0.05);②与冷应激组相比,日粮中添加500 mg/kg三乳酸甘油酯对冷应激肉鸡生长性能和器官相对重量并无影响,但可提高心脏GSH-Px、肝脏SOD和肺脏CAT的活力、心脏ATP和EC值、心脏和肝脏ATP5B mRNA水平(P<0.05),并降低心脏AMP/ATP比值、HMOX-1和AMPK-α1 mRNA水平(P<0.05).研究结果表明:三乳酸甘油酯可提高冷应激肉鸡的心脏、肝脏及肺脏抗氧化能力并改善心脏的能量代谢状况.
语种:
中文
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L-精氨酸和α-酮戊二酸对热应激肉鸡肠道吸收功能、抗氧化能力、能量代谢的影响
作者:
晏利琼;廖满;谢佳倩;谭灵琳;丁斌鹰;...
期刊:
中国畜牧杂志 ,2016年(15):33-41 ISSN:0258-7033
作者机构:
1. 动物营养与饲料安全湖北省协同创新中心动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院;2. 中国农业大学动物科技学院
关键词:
L-精氨酸;α-酮戊二酸;热应激;肠道;肉鸡
摘要:
本试验旨在研究L-精氨酸(Arg)和α-酮戊二酸(AKG)对热应激肉鸡肠道功能的影响。选用200只1日龄Cobb肉鸡,随机分为4个处理组:对照组(基础日粮)、热应激组(基础日粮)、Arg组(基础日粮+0.5%Arg)、AKG组(基础日粮+0.5%AKG),每个组5个重复,每个重复10只鸡。试验为期5周,第1周各组室温控制在32~34℃,第2~5周热应激组、Arg组和AKG组控制在(36±2)℃,而对照组第2周开始慢慢降至(22±2)℃,并从第3周开始维持在(22±2)℃,湿度为50%~60%。结果表明:与对照组相比,热应激处理降低了肉鸡生长性能和血液三碘甲腺原氨酸(T3)含量(P<0.05),同时也显著降低了空肠和回肠脂肪酶、回肠胰蛋白酶、空肠过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)、回肠超氧化物歧化酶(SOD)和CAT的活力、十二指肠和回肠三磷酸腺苷(ATP)含量、空肠小肽转运载体(Pep T1)、紧密连接蛋白(Claudin-5)m RNA表达量(P<0.05),显著提高了死亡率、血液皮质酮、十二指肠和回肠丙二醛(MDA)含量、空肠低氧诱导因子-1α(HIF-1α)、AMP依赖的蛋白激酶α1(AMPK-α1)m RNA表达量(P<0.05);与热应激组相比,日粮中添加0.5%Arg可显著提高热应激肉鸡饲料转化率、十二指肠脂肪酶和回肠胰蛋白酶活力、十二指肠和空肠DNA含量、空肠CAT活力和HMOX-1 m RNA表达量(P<0.05),降低死亡率、十二指肠和空肠RNA/DNA比值、空肠TP/DNA比值、HIF-1α和AMPK-α1 m RNA表达量(P<0.05),而日粮中添加0.5%AKG可显著提高热应激肉鸡血液T3、十二指肠和空肠DNA含量、十二指肠CAT和GSH-Px的活力、十二指肠和空肠及回肠ATP含量(P<0.05),并显著降低死亡率、血液皮质酮含量、空肠RNA/DNA及TP/DNA比值、十二指肠MDA和回肠过氧化氢(H2O2)含量(P<0.05)。因此,日粮中添加0.5%Arg可提高热应激肉鸡肠道消化能力和抗氧化功能,而添加0.5%AKG可促进热应激肉鸡肠道黏膜细胞生长,提高肠道抗氧化能力,并改善肠道能量代谢状况。
语种:
中文
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Gene expression profiles in the intestine of lipopolysaccharide-challenged piglets
作者:
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Zhao, Di;Ding, Binying;...
期刊:
Frontiers in Bioscience-Landmark ,2016年21(3):487-501 ISSN:2768-6701
通讯作者:
Hou, Yongqing
作者机构:
[Wu, Tao; Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Gene expression;Intestinal function;Lipopolysaccharide;Pig;Review
摘要:
Bowel diseases are common in human and animals and are characterized by intestinal dysfunction and injury. A well-established porcine model of intestinal injury can be induced by lipopolysaccharide (LPS), an endotoxin released from the cell wall of pathogenic bacteria. LPS affects the expression of genes associated with intestinal immune response, mucosal growth, energy metabolism, absorption, mucosal barrier function, and antiviral function. Transcriptional analysis of intestinal genes reveals that the duodenum, jejunum, ileum and colon respond to LPS challenge in a similar pattern. Moreover, the jejunum and ileum exhibit greater responses to LPS challenge than the duodenum and colon with regard to gene expression. Additionally, over 85% of genes are co-expressed along the small and large intestines and there is a clear distinction in gene expression patterns amongst the different intestinal segments in pigs. These findings have important implications for underlying molecular mechanisms responsible for endotoxin-induced intestinal injury and dysfunction.
语种:
英文
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抑制大肠杆菌K88的益生菌体外筛选
作者:
王磊;吕阳;张越;谢佳倩;赵迪;...
期刊:
中国畜牧兽医 ,2016年(05):1355-1360 ISSN:1671-7236
作者机构:
1. 武汉轻工大学动物科学与营养工程学院;2. 动物营养与饲料科学湖北省重点实验室
关键词:
益生菌;干酪乳杆菌;凝结芽孢杆菌;IPEC-1细胞系;LDH活性;平板计数法
摘要:
本研究以从断奶仔猪肠道分离的益生菌为试验菌株,将大肠杆菌K88和益生菌接种到体外培养的猪小肠上皮细胞(intestinal porcine epithelial cell-1,IPEC-1)中,测定培养2.5h上清液中的乳酸脱氢酶(lactate dehydrogenase,LDH)活性,同时将益生菌和大肠杆菌K88体外混合培养2.5h,进行平板计数,统计大肠杆菌K88菌数的变化,筛选出可以抑制大肠杆菌K88的益生菌。试验结果显示,干酪乳杆菌和凝结芽孢杆菌能显著降低IPEC-1培养上清液中的LDH活性(P<0.05),降低大肠杆菌K88对细胞的损伤;凝结芽孢杆菌能降低DMEM培养基中大肠杆菌K88的生长速度,结合模拟制粒过程及胃肠道环境的耐高温、耐酸及耐胆盐研究进行综合分析,该凝结芽胞杆菌对大肠杆菌K88有较好的抑制作用且具有良好的耐高温、耐酸及耐胆盐性能,具有作为微生态制剂菌株的应用潜力。本试验建立了能够抑制大肠杆菌K88的益生菌体外筛选技术模型。
语种:
中文
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大豆抗原蛋白对仔猪肠道转运通道的影响
作者:
梅慧敏;周颖;张越;谢佳倩;周佳;...
作者机构:
[梅慧敏; 周颖; 张越; 谢佳倩; 周佳; 李抗; 李宝成; 易丹; 丁斌鹰; 王蕾] 武汉轻工大学动物营养与饲料科学湖北省重点实验室;[梅慧敏; 周颖; 张越; 谢佳倩; 周佳; 李抗; 李宝成; 易丹; 丁斌鹰; 王蕾] 武汉轻工大学动物营养与饲料安全湖北省协同创新中心
会议名称:
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间:
2016-10-21
会议地点:
中国湖北武汉
关键词:
大豆抗原蛋白;仔猪;生长性能;肠道功能
摘要:
本试验研究了大豆抗原蛋白对仔猪生长性能及肠道功能的影响。试验选用21日龄(杜×长×大)仔猪12头,随机分成2个处理组:对照组和大豆分离蛋白组,每组6个重复,每个重复1头猪。对照组饲喂含4%酪蛋白的基础日粮,大豆分离蛋白组饲喂含4%大豆分离蛋白的基础日粮。试验期为7天,于试验第7天采血后屠宰,取十二指肠、空肠、回肠等组织样品,测定仔猪生长性能、血液生化指标、肠道损伤和肠道功能相关基因等指标。结果表明
语种:
中文
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Dietary Supplementation with α-Ketoglutarate Activates mTOR Signaling and Enhances Energy Status in Skeletal Muscle of Lipopolysaccharide-Challenged Piglets
作者:
Wang, Lei;Yi, Dan;Hou, Yongqing* ;Ding, Binying;Li, Kang;...
期刊:
The Journal of Nutrition ,2016年146(8):1514-1520 ISSN:0022-3166
通讯作者:
Hou, Yongqing;Wu, Guoyao
作者机构:
[Li, Kang; Liu, Yulan; Li, Baocheng; Hou, Yongqing; Wu, GY; Wang, Lei; Wu, Guoyao; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, YQ; Wu, GY] W;[Wu, Guoyao] T;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Peoples R China.;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
关键词:
*cell signaling;*gastrocnemius muscle;*inflammation;*lipopolysaccharide;*alpha-ketoglutarate
摘要:
Background: Skeletal muscle undergoes rapid loss in response to inflammation. a-Ketoglutarate (AKG) has been reported to enhance muscle growth in piglets, but the underlying mechanisms are largely unknown. Objectives: This study tested the hypothesis that dietary AKG supplementation activates mechanistic target of rapamycin (mTOR) signaling and improves skeletal muscle energy metabolism in lipopolysaccharide (LPS)-challenged piglets. Methods: Forty-eightmale piglets (Duroc × Landrace × Yorkshire)wereweaned at 21 d of age to a corn- and soybeanmeal- based diet. After a 3-d period of adaptation, piglets with a mean weight of 7.21 kg were randomly assigned to control, LPS (intraperitoneal administration of 80 μg LPS/kg body weight on days 10, 12, 14, and 16), or LPS plus 1% dietary AKG (LPS +AKG) groups. On day 16, blood sampleswere collected from8 piglets/group 3 h after LPS administration.On day 17, piglets were killed to obtain gastrocnemius muscle from 8 piglets/group for biochemical analysis. Results: Compared with the control group, LPS administration increased (P < 0.05) plasma concentrations of globulin (by 14%) and tumor necrosis factor α (by 59%) and the intramuscular ratio of AMP to ATP (by 93%) and abundance of phosphorylated acetyl- coenzyme A carboxylase (ACC) β protein (by 64%). Comparedwith the control group, LPS administration reduced (P < 0.05) weight gain (by 15%); plasma concentrations of glutamine (by 20%), glucose (by 23%), insulin, insulin-like growth factor I, and epidermal growth factor; intramuscular concentrations of glutamine (by 27%), ATP (by 12%), ADP (by 22%), and total adenine nucleotides; and intramuscular ratios of phosphorylated mTOR to total mTOR (by 38%) and of phosphorylated 70-kDa ribosomal protein S6 kinase (p70S6K) to total p70S6K (by 39%). These adverse effects of LPS were ameliorated (P < 0.05) by AKG supplementation. Conclusions: Dietary AKG supplementation activated mTOR signaling, inhibited ACC-β, and improved energy status in skeletal muscle of LPS-challenged piglets. These results provide a biochemical basis for the use of AKG to enhance piglet growth under inflammatory or practical postweaning conditions. © 2016 American Society for Nutrition.
语种:
英文
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N-acetylcysteine improves the growth performance and intestinal function in the heat-stressed broilers
作者:
Yi, Dan;Hou, Yongqing;Tan, Linglin;Liao, Man;Xie, Jiaqian;...
期刊:
Animal Feed Science and Technology ,2016年220:83-92 ISSN:0377-8401
通讯作者:
Ding, Binying
作者机构:
[Gong, Joshua; Liao, Man; Hou, Yongqing; Tan, Linglin; Xie, Jiaqian; Wang, Lei; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Yang, Ying] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.;[Ding, Binying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Ding, Binying] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Broiler;Heat stress;Intestinal function;N-acetylcysteine
摘要:
This study was carried out to investigate the effects of N-acetylcysteine (NAC) on growth performance and intestinal function of heat-stressed broilers. A total of two hundred 1-d-old Cobb male chicks were allocated to 1 of 4 treatments, with 5 replicated pens per treatment and 10 birds per pen. The experiment consisted of 4 treatments in a 2 × 2 factorial arrangements with two diets (basal diet or 1 g/kg NAC diet) and two temperatures (thermoneutral or heat stress). From day 8–35 of age, broilers were raised at thermoneutral (26 ± 1 °C) or exposed to cyclic heat stress (36 ± 1 °C from 0800 to 1800 and 26 ± 1 °C from 1800 to 0800). The results showed that heat stress reduced ADFI, ADG, plasma concentrations of triiodothyronine (T3) and thyroxine (T4), intestinal villus height (VH), ratio of VH to crypt depth (CD), intestinal mucosal ATP level, adenylate energy charge (AEC), activities of alkaline phosphatase (AKP), antioxidative and digestive enzymes, and mRNA level for Bcl-2, whereas increased the feed/gain, mortality rate, plasma corticosterone level, intestinal CD, intestinal mucosal AMP and malondialdehyde (MDA) levels, and mRNA levels of heat shock protein (HSP70), caspase-3, AMP-activated protein kinase (AMPK), heme-oxigenase (HMOX), and xanthine oxidoreductase (XOR). Dietary supplementation with NAC decreased the feed/gain, mortality rate, plasma corticosterone level, MDA concentration and intestinal mucosal mRNA levels of HSP70, AMPK and HMOX, while elevated the ratio of VH to CD, ATP, catalase (CAT) and trypsine activity in the small intestine of heat-stressed broilers. Taken together, these results suggest that dietary supplementation of 1 g/kg NAC could improve growth performance and intestinal function of broilers exposed to heat stress. © 2016 Elsevier B.V.
语种:
英文
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肉桂醛对白羽肉鸡血液生化指标及免疫器官指数的影响
作者:
段瑞;丁斌鹰;李叶涵;毕云天
作者机构:
[段瑞; 丁斌鹰; 李叶涵; 毕云天] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称:
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间:
2016-10-21
会议地点:
中国湖北武汉
关键词:
肉桂醛;肉仔鸡;血液生化指标;免疫器官指数
摘要:
肉桂醛是一种植物混合提取物,在动物消化道内有较好的抑菌效果,有研究报道其可以优化肠道结构,提高肉鸡的免疫机能,促进肠道有益菌数量增加,降低肠道有害菌数量,改善动物的健康状况。本试验旨在研究饲粮中添加不同浓度的包被肉桂醛对不同阶段白羽肉鸡血细胞、血液生化指标以及免疫器官指数的影响。选取1日龄平均体重为45.23 g的科宝(cobb)肉仔鸡450只,随机分为5组,其中对照组为玉米-豆粕型基础饲粮,其他
语种:
中文
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三乳酸甘油酯对冷应激肉鸡肠道抗氧化能力和能量代谢状况的影响
作者:
廖满;段瑞;谢佳倩;周建;杨书慧;...
期刊:
中国畜牧杂志 ,2016年(13):47-54 ISSN:0258-7033
作者机构:
动物营养与饲料安全湖北省协同创新中心动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院
关键词:
乳酸甘油酯;冷应激;抗氧化;能量代谢;肉鸡
摘要:
本试验研究三乳酸甘油酯对冷应激肉鸡肠道的抗氧化能力及能量代谢状况的影响。试验选用150只1日龄Cobb肉鸡,随机分为3个处理组:对照组、冷应激组、乳酸甘油酯组,分别饲喂基础日粮、基础日粮、基础日粮+500mg/kg三乳酸甘油酯,每组5个重复,每个重复10只鸡。试验第1周各组温度控制在32~34℃,第2周控制在30~32℃,试验第3~6周冷应激组、乳酸甘油酯组控制在(10±2)℃,而对照组维持在(26±2)℃,湿度50%~60%。结果表明:与对照组相比,冷应激处理降低了肉鸡生长性能、十二指肠过氧化氢酶(CAT)、空肠超氧化物歧化酶(SOD)、CAT及谷胱甘肽过氧化物酶(GSH-Px)的活力(P<0.05),同时也降低了十二指肠的三磷酸腺苷(ATP)、腺苷酸池(TAN)和能荷(EC)含量,回肠的ATP、二磷酸腺苷(ADP)和EC含量及空肠血红素加氧酶-1(HMOX-1)m RNA相对表达量(P<0.05),但提高了十二指肠和回肠AMP/ATP比值,同时也提高了十二指肠、空肠、回肠AMP依赖的蛋白激酶-α1(AMPK-α1)m RNA相对表达量及十二指肠低氧诱导因子-1(HIF-1)m RNA相对表达量(P<0.05);与冷应激组相比,日粮中添加500 mg/kg三乳酸甘油酯对冷应激肉鸡生长性能并无影响,但提高了冷应激肉鸡肠道SOD活力、十二指肠CAT和空肠GSH-Px活力、十二指肠和回肠ATP、EC含量、十二指肠和回肠血红素加氧酶-1(HMOX-1)m RNA及空肠HIF-1、COXⅢm RNA相对表达量(P<0.05),同时降低了十二指肠和回肠AMP/ATP比值,十二指肠、空肠及回肠AMPK-α1m RNA相对表达量、十二指肠和回肠HIF-1 m RNA相对表达量及空肠PGC-1αm RNA相对表达量(P<0.05);本试验结果表明,三乳酸甘油酯可提高冷应激肉鸡的肠道抗氧化能力、改善肠道的能量代谢状况,这可能与三乳酸甘油酯调控HIF-1/HMOX-1和AMPK信号通路有关。
语种:
中文
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L-精氨酸和α-酮戊二酸对热应激肉鸡肝脏功能的影响
作者:
李叶涵;周佳;张越;谭灵琳;廖满;...
期刊:
饲料工业 ,2016年37(16):6-11 ISSN:1001-991X
作者机构:
动物营养与饲料安全湖北省协同创新中心 动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院,湖北武汉,430023;[丁斌鹰; 杨书慧; 周佳; 廖满; 谭灵琳; 张越; 李叶涵] 武汉轻工大学
关键词:
L-精氨酸;α-酮戊二酸;热应激;肝脏;肉鸡
摘要:
试验旨在研究L-精氨酸(Arg)和α-酮戊二酸(AKG)对热应激肉鸡肝脏功能的影响。选用200只1日龄科宝肉鸡,随机分为4个处理组:对照组(基础日粮)、热应激组(基础日粮)、Arg组(基础日粮+0.5%Arg)、AKG组(基础日粮+0.5%AKG),每组5个重复,每个重复10只鸡。试验期5周。结果表明:1与对照组相比,热应激处理降低了肉鸡肝脏相对重量、血液三碘甲状腺原氨酸(T3)和四碘甲状腺原氨酸(T4)含量、肝脏三磷酸腺苷(ATP)、腺苷酸池(TAN)含量及能荷(EC)值(P〈0.05),同时也降低了肝脏过氧化物酶体增殖活化受体γ辅助活化因子1α(PGC-1α)、血红素加氧酶-1(HMOX-1)和黄嘌呤氧化还原酶(XOR)的m RNA水平(P〈0.05),但提高了血液肌酸激酶(CK)和乳酸脱氢酶(LDH)活性、皮质酮含量、肝脏丙二醛(MDA)和过氧化氢(H2O2)含量(P〈0.05);2与热应激组相比,日粮中添加0.5%Arg可提高热应激肉鸡血液T3和T4含量、肝脏ATP含量、肝脏PGC-1α、HMOX-1和XOR的m RNA水平(P〈0.05),但降低了血液CK活性、皮质酮含量、肝脏H2O2含量及低氧诱导因子1α(HIF-1α)的m RNA水平(P〈0.05);3与热应激组相比,日粮中添加0.5%的AKG可提高血液T3含量,肝脏相对重量、二磷酸腺苷(ADP)和TAN含量及EC值(P〈0.05),但降低了血液皮质酮含量、肝脏H2O2含量、一磷酸腺苷(AMP)依赖的蛋白激酶α1(AMPK-α1)和PGC-1α的m RNA水平(P〈0.05)。因此,日粮中添加0.5%Arg可改善热应激肉鸡肝脏能量代谢状况和抗氧化能力,提高肉鸡抗热应激能力,而0.5%AKG能改善热应激肉鸡肝脏的能量代谢状况和提高肝脏相对重量。
语种:
中文
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三乳酸甘油酯和橄榄油对冷应激肉鸡生长性能、血液生化指标及抗氧化能力的影响
作者:
廖满;段瑞;谢佳倩;谭灵琳;易丹;...
期刊:
饲料工业 ,2016年37(10):13-17 ISSN:1001-991X
作者机构:
动物营养与饲料安全湖北省协同创新中心 动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院,湖北武汉,430023;[丁斌鹰; 杨书慧; 段瑞; 廖满; 谭灵琳; 易丹; 谢佳倩] 武汉轻工大学
关键词:
三乳酸甘油酯;橄榄油;冷应激;肉鸡;血液生化指标;抗氧化
摘要:
试验旨在研究三乳酸甘油酯和橄榄油对冷应激肉鸡生长性能、血液生化指标及血液抗氧化能力的影响。选用200只1日龄科宝肉鸡,随机分为4个处理组:对照组、冷应激组、乳酸甘油酯组、橄榄油组,分别饲喂基础日粮、基础日粮、基础日粮+500 mg/kg三乳酸甘油酯、以橄榄油替代豆油的基础日粮,每组5个重复,每个重复10只鸡。试验第1周各组温度控制在32~34℃,第2周控制在30~32℃,试验第3~6周冷应激组、乳酸甘油酯组、橄榄油组控制在(10±2)℃,而对照组维持在(26±2)℃,湿度50%~60%。试验结果表明:①与对照组相比,冷应激处理降低了肉鸡的生长性能、部分组织器官相对重量(胸腺、法氏囊、腿肌)和血液超氧化物歧化酶(SOD)的酶活力(P〈0.05),提高了血液碱性磷酸酶(ALP)的酶活力和心脏的相对重量(P〈0.05);②与冷应激组相比,日粮中添加三乳酸甘油酯降低了冷应激肉鸡血液谷丙转氨酶(ALT)、ALP的酶活力和总胆红素(TBIL)含量(P〈0.05),提高了血液SOD和谷胱甘肽过氧化物酶(GSH-Px)的酶活力(P〈0.05);③与冷应激组相比,日粮中橄榄油替代豆油可提高冷应激肉鸡血液的总蛋白(TP)、球蛋白(GLB)含量及血液SOD和GSH-Px的酶活力(P〈0.05),降低血液TBIL含量(P〈0.05)。试验研究结果表明,三乳酸甘油酯和橄榄油均能够改善冷应激肉鸡血液生化指标,提高冷应激肉鸡的血液抗氧化能力。
语种:
中文
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黄曲霉毒素生物合成及代谢转换的研究进展
作者:
熊江林;周华林;丁斌鹰;刘建新
期刊:
家畜生态学报 ,2015年36(04):85-89 ISSN:1673-1182
作者机构:
武汉轻工大学动物科学与营养工程学院,湖北武汉430023;浙江大学奶业科学研究所,浙江杭州310058;襄阳职业技术学院生物工程学院,襄阳市动物医学工程技术中心,湖北襄阳441100;武汉轻工大学动物科学与营养工程学院,湖北武汉,430023;浙江大学奶业科学研究所,浙江杭州,310058
关键词:
黄曲霉毒素;合成途径;代谢转化;研究进展
摘要:
黄曲霉毒素(Aflatoxins,AFs)是真菌产生的有机代谢产物,具有强烈的生物毒性,广泛存在于饲料原料和各类饲料之中。本文综述了AFs的产生条件、合成途径以及AFB1在动物体内的代谢转化,旨为黄曲霉毒素的防控和动物健康养殖提供参考。
语种:
中文
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Dietary supplementation with glutamate precursor α-ketoglutarate attenuates lipopolysaccharide-induced liver injury in young pigs
作者:
Wang, Lei;Hou, Yongqing* ;Yi, Dan;Li, Yongtang;Ding, Binying;...
期刊:
Amino Acids ,2015年47(7):1309-1318 ISSN:0939-4451
通讯作者:
Hou, Yongqing
作者机构:
[Xiao, Hang; Hou, Yongqing; Liu, Jian; Wang, Lei; Li, Yongtang; Wu, Guoyao; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
alpha-Ketoglutarate;Piglets;Lipopolysaccharide;Liver injury
摘要:
Abstract There is growing interest in glutamate as a functional amino acid in nutrition and health. This study was conducted to determine whether glutamate precursor α-ketoglutarate (AKG) could alleviate lipopolysaccharide (LPS)-induced liver injury in young pigs. Twenty-four piglets were randomly assigned to the control, LPS, or LPS + AKG group. Piglets in the control and LPS groups were fed a basal diet, whereas piglets in the NAC group were fed the basal diet supplemented with 1 % AKG. On days 10, 12, 14, and 16 of the trial, piglets in the LPS and LPS + AKG groups received intraperitoneal administration of LPS (80 μg/kg BW), whereas piglets in the control group received the same volume of saline. On day 16 of the trial, blood samples were collected 3 h after LPS or saline injection. Twenty-four hours post-administration of LPS or saline (on day 17 of the trial), piglets were killed to obtain liver for analysis. Dietary AKG supplementation alleviated LPS-induced histomorphological abnormalities and mitigated LPS-induced increases in aspartate aminotransferase (AST) activity and AST/ALT ratio (P < 0.05). Compared with the LPS group, dietary supplementation with AKG decreased plasma glutamate concentration, while increasing hepatic concentrations of glutamate, glutamine, leucine, asparagine, lysine, alanine, serine, threonine, valine, and phenylalanine (P < 0.05). LPS challenge dramatically increased concentrations of malondialdehyde and decreased glutathione peroxidase activity in the liver. Additionally, LPS challenge enhanced concentrations of AMP and total protein, as well as RNA/DNA and total protein/DNA ratios, while decreasing hepatic ADP concentrations. These adverse effects of LPS challenge were ameliorated by AKG supplementation. Collectively, dietary AKG supplementation provides a new means to ameliorate LPS-induced liver injury by increasing anti-oxidative capacity and improving energy metabolism in young pigs. © 2015 Springer-Verlag Wien.
语种:
英文
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绿色荧光蛋白穿梭载体的构建及其在嗜酸乳杆菌中的应用
作者:
曾娟娟;王磊;赵迪;吕阳;王蕾;...
期刊:
中国畜牧兽医 ,2015年42(10):2551-2559 ISSN:1671-7236
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;[丁斌鹰; 陈洪波; 侯永清; 吕阳; 王蕾; 曾娟娟; 易丹; 赵迪; 王磊] 武汉轻工大学
关键词:
嗜酸乳杆菌;同源重组;绿色荧光蛋白
摘要:
为深入研究嗜酸乳杆菌的作用机理,以温敏型穿梭载体pSET4s为基本骨架,将绿色荧光蛋白(GFP)和嗜酸乳杆菌上、下游同源臂插入该载体,构建GFP标记的穿梭表达载体pSET4s-P1-GFP-P2。利用电转化方法将穿梭表达载体pSET4s-P1-GFP-P2转化入嗜酸乳杆菌,通过抗性和温度双重筛选获得能稳定表达GFP的重组嗜酸乳杆菌,将Western blotting和荧光显微镜观察验证的重组菌命名为ΔMG6243(GFP)。利用荧光显微镜观察和平板计数的方法对重组菌进行遗传稳定性和生物学特性研究。结果显示,重组菌在蓝光激发下可见明显绿色荧光,连传10代仍可见明显荧光,与野生菌相比,其生长特性、酸碱盐耐受性均无显著差别。结果表明,本试验已成功构建稳定表达GFP基因的重组嗜酸乳杆菌,利用温敏型穿梭载体pSET4s实现了外源基因在嗜酸乳杆菌中非抗性、整合型表达,为嗜酸乳杆菌基因重组菌的构建奠定了基础,同时GFP的标记作用也为嗜酸乳杆菌在动物体内分布、定植规律和作用机理创造了可行的条件。
语种:
中文
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L-Glutamine enhances enterocyte growth via activation of the mTOR signaling pathway independently of AMPK
作者:
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Ouyang, Wanjin;Long, Minhui;...
期刊:
Amino Acids ,2015年47(1):65-78 ISSN:0939-4451
通讯作者:
Hou, Yongqing
作者机构:
[Ouyang, Wanjin; Long, Minhui; Liu, Yulan; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词:
AMPK;IPEC-1;l-Glutamine;mTOR
摘要:
Neonates (including human infants) require l-glutamine (Gln) for optimal intestinal health. This study tested the hypothesis that Gln enhances enterocyte growth via both mammalian target of rapamycin (mTOR) and AMP-activated kinase (AMPK) signaling pathways. Intestinal porcine epithelial cells (IPEC-1) were cultured for 3 days in Gln-free Dulbecco's modified Eagle medium containing 0 or 2 mM Gln. To determine the role of mTOR and AMPK on cell growth, additional experiments were conducted where medium contained 2 mM Gln and 10 nM rapamycin (Rap, an inhibitor of mTOR) or 1 μM compound C (an inhibitor of AMPK). IPEC-1 cell growth increased with increasing concentrations of Gln from 0 to 2 mM. Compared with 0 mM Gln, 2 mM Gln increased (P < 0.05) the amounts of phosphorylated 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase (p70S6 kinase) proteins but did not affect abundances of total or phosphorylated AMPK protein. Gln also increased mRNA levels for Bcl-2, mTOR, p70S6 kinase, 4E-BP1, COX7C, ASCT2, ODC, SGLT-1, CFTR, Na+/K+-ATPase, HSP70, and ZO-1. Similarly, cells cultured with Rap and Gln exhibited higher (P < 0.05) abundances of phosphorylated 4E-BP1 and p70S6 kinase proteins than the Rap-only group, whereas abundances of phosphorylated mTOR and 4E-BP1 proteins were increased when AMPK was inhibited by compound C. Conversely, the amount of phosphorylated AMPK increased when mTOR was inhibited by Rap, suggesting a negative cross-talk between mTOR and AMPK. Collectively, these results indicate that Gln stimulates enterocyte growth by activating the mTOR signaling pathway independently of AMPK. © 2014 Springer-Verlag Wien.
语种:
英文
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Beneficial roles of dietary oleum cinnamomi in alleviating intestinal injury
作者:
Wang, Lei;Hou, Yongqing* ;Yi, Dan;Ding, Binying;Zhao, Di;...
期刊:
Frontiers in Bioscience-Landmark ,2015年20(5):814-828 ISSN:2768-6701
通讯作者:
Hou, Yongqing
作者机构:
[Gong, Joshua; Liu, Yulan; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Ding, Binying; Zhao, Di; Yi, Dan; Wang, Zhongxing; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao; Assaad, Houssein] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Intestinal mucosa;Lipopolysaccharide;Oleum cinnamomi;Piglets;Review
摘要:
Cinnamon is a traditional herb used for treatment of many human diseases. The most important chemical compounds of the essential oil are cinnamaldehyde and eugenol. Oleum cinnamomi (OCM, cinnamon oil) is increasingly used as a feed additive to animal diets. Beneficial effects of OCM in protecting tissues from inflammation and injury by endogenous and exogenous agents (such as hydrogen peroxide and lipopolysaccharide (LPS)) may result, in part, from its action on regulating amino acid metabolism in cells to favor the synthesis of glutathione (a major low-molecular-weight antioxidant) from cysteine, glycine and glutamate. In support of this notion, results of recent studies indicate that supplementing OCM (50 mg/kg diet) to a corn- and soybean meal-based diet for piglets weaned at 21 days of age enhances intestinal anti-oxidative capacity and reduces the incidence of diarrhea. Additionally, dietary supplementation with OCM ameliorates LPS-induced mucosal barrier dysfunction and mucosal damage in the small intestine. OCM holds great promise for protecting the gut from injury under conditions of inflammation, infections, and oxidative stress. © 2015, Frontiers in Bioscience. All rights reserved.
语种:
英文
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Effects of tributyrin on intestinal energy status, antioxidative capacity and immune response to lipopolysaccharide challenge in broilers
作者:
Li, Jiaolong;Hou, Yongqing;Yi, Dan;Zhang, Jun;Wang, Lei;...
期刊:
Animal Bioscience ,2015年28(12):1784-1793 ISSN:2765-0189
通讯作者:
Ding, Binying
作者机构:
[Qin, Hongyi; Hou, Yongqing; Li, Jiaolong; Wang, Lei; Ding, Binying; Yi, Dan; Zhang, Jun] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Ding, Binying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Ding, Binying] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Li, Jiaolong;Hou, Yongqing;Yi, Dan;Zhang, Jun;Wang, Lei;Qiu, Hongyi;Ding, Binying;Gong, Joshua;Tributyrin;Lipopolysaccharide;Intestine;Immune Response;Broilers
摘要:
This study was carried out to investigate the effects of tributyrin (TB) on the growth performance, pro-inflammatory cytokines, intestinal morphology, energy status, disaccharidase activity, and antioxidative capacity of broilers challenged with lipopolysaccharide (LPS). A total of 160 one-day-old Cobb broilers were allocated to 1 of 4 treatments, with 4 replicated pens per treatment and 10 birds per pen. The experiment consisted of a 2×2 factorial arrangements of treatments with TB supplementation (0 or 500 mg/kg) and LPS challenge (0 or 500 μg/kg body weight [BW]). On days 22, 24, and 26 of the trial, broilers received an intraperitoneal administration of 500 μ g/kg BW LPS or saline. Dietary TB showed no effect on growth performance. However, LPS challenge decreased the average daily gain of broilers from day 22 to day 26 of the trial. Dietary TB supplementation inhibited the increase of interleukin-1β (in the jejunum and ileum), interleukin-6 (in the duodenum and jejunum), and prostaglandin E2 (in the duodenum) of LPS-challenged broilers. Similar inhibitory effects of TB in the activities of total nitric oxide synthase (in the ileum) and inducible nitric oxide synthase (in the jejunum) were also observed in birds challenged with LPS. Additionally, TB supplementation mitigated the decrease of ileal adenosine triphosphate, adenosine diphosphate and total adenine nucleotide and the reduction of jejunal catalase activity induced by LPS. Taken together, these results suggest that the TB supplementation was able to reduce the release of pro-inflammatory cytokines and improve the energy status and anti-oxidative capacity in the small intestine of LPS-challenged broilers. Copyright © 2015 by Asian-Australasian Journal of Animal Sciences.
语种:
英文
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N-乙酰半胱氨酸对肉鸭肝脏能量代谢状况及组织抗氧化指标的影响
作者:
谭灵琳;侯永清;易丹;廖满;王蕾;...
期刊:
中国畜牧杂志 ,2015年51(17):53-58 ISSN:0258-7033
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[丁斌鹰; 廖满; 侯永清; 谭灵琳; 王蕾; 易丹] 武汉轻工大学
关键词:
N-乙酰半胱氨酸;能量代谢;抗氧化能力;肉鸭
摘要:
为考查N-乙酰半胱氨酸(NAC)是否可以改善肉鸭肝脏的能量代谢状况及组织抗氧化能力,试验选用90只1 d樱桃谷肉鸭,随机分成3个处理组:对照组(基础日粮)、500 mg/kg NAC组(基础日粮+500 mg/kg NAC)、1 000 mg/kg NAC组(基础日粮+1 000 mg/kg NAC),每组3个重复,每个重复10只鸭。试验期为3周(1~21日龄)。结果表明:与对照组相比,日粮添加500 mg/kg NAC显著提高了血浆、肝脏的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活力及肝脏谷胱甘肽过氧化物酶(GSH-Px)的酶活力、三磷酸腺苷(ATP)、二磷酸腺苷(ADP)的含量和能荷(EC)值(P<0.05),显著降低了血浆丙二醛(MDA)和肝脏过氧化氢(H2O2)的含量及肝脏AMP/ATP值和肝脏低氧诱导因子1(HIF-1)、黄嘌呤氧化还原酶(XOR)的m RNA水平(P<0.05);1 000 mg/kg NAC组显著提高了血浆和肝脏CAT、SOD的活力及肝脏GSH-Px的酶活力、ATP的含量、EC值和AMPK-α1的m RNA水平(P<0.05),显著降低了血浆和肝脏H2O2的含量及肝脏MDA的含量、HIF-1、XOR的m RNA水平。表明NAC可以提高肉鸭的组织抗氧化能力、改善肝脏的能量代谢,这可能与NAC对AMPK-α1、HIF-1及XOR的基因表达调控有关。
语种:
中文
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三丁酸甘油酯与三乳酸甘油酯对脂多糖刺激肉鸡生长性能、血液生化指标及肝脏能量状态的影响
作者:
李娇龙;张俊;邱洪仪;杨玲;谭灵琳;...
期刊:
饲料工业 ,2014年35(18):38-43 ISSN:1001-991X
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室;[杨玲] 武汉泛华生物技术有限公司;[丁斌鹰; 李娇龙; 谭灵琳; 张俊; 邱洪仪] 武汉轻工大学
关键词:
三丁酸甘油酯;三乳酸甘油酯;生长性能;能量状态;脂多糖;肉鸡
摘要:
试验研究了三丁酸甘油酯与三乳酸甘油酯对1~21日龄肉鸡生产性能和对脂多糖(LPS)刺激肉鸡生长性能、血浆GPT、GOT、AKP活性以及肝脏能量状态的影响.试验分2期.第Ⅰ期:选取240只体重基本一致的1日龄Cobb肉公鸡,随机分成3组(对照组、丁酸酯组和乳酸酯组),每组4个重复,每个重复20只鸡.其中对照组饲喂基础日粮,丁酸酯组和乳酸酯组分别饲喂在基础日粮的基础上添加0.1%三丁酸甘油酯、0.1%三乳酸甘油酯的试验日粮.第Ⅱ期:在试验第22 d,试验分为4组,分别为对照组和LPS组(选自试验Ⅰ期对照组,各10只鸡)、丁酸酯组和乳酸酯组(从试验Ⅰ期丁酸酯组和乳酸酯组各选取10只鸡).其中对照组和LPS组饲喂基础日粮,丁酸酯组和乳酸酯组分别饲喂在基础日粮的基础上添加0.1%三丁酸甘油酯、0.1%三乳酸甘油酯的试验日粮.每个组10个重复,每个重复1只鸡.在试验第25、27、29 d上午8:00,对LPS组、丁酸酯组和乳酸酯组的试验肉鸡分别腹腔注射500 μg/kg体重的LPS,对照组注射相同剂量的灭菌生理盐水.第29 d中午12:00采血,第30 d上午8:00屠宰取肝脏.试验Ⅰ期结果显示:饲粮中添加0.1%的三丁酸甘油酯和三乳酸甘油酯对1~21日龄肉鸡生长性能均无显著性影响(P>0.05).试验Ⅱ期结果显示:①饲粮中添加三丁酸甘油酯和三乳酸甘油酯均缓解了LPS刺激引起了日增重的降低(P>0.05),且二种甘油三酯之间无显著性差异(P>0.05).②饲粮中添加三丁酸甘油酯和三乳酸甘油酯均缓解了LPS刺激导致的血浆谷丙转氨酶(GPT)、谷草转氨酶(GOT)的升高(P<0.05),但二种甘油三酯之间并无显著性差异(P>0.05).③饲粮中添加三丁酸甘油酯和三乳酸甘油酯均显著缓解了LPS刺激引起的三磷酸腺苷(ATP)、二磷酸腺苷(AMP)、腺苷酸池(TAN)、能荷(EC)的降低(P<0.05)和AMP/ATP的升高(P<0.05).三乳酸甘油酯还显著升高了AMP水平(P<0.05).由此可见,在本试验条件下,饲粮中添加0.1%的三丁酸甘油酯和三乳酸甘油酯均缓解了LPS刺激引起的肝脏损伤.
语种:
中文
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α-酮戊二酸对热应激肉鸡生长性能和肌肉品质的影响
作者:
杨书慧;谭灵琳;廖满;丁斌鹰;易丹;...
期刊:
饲料工业 ,2014年35(22):11-16 ISSN:1001-991X
作者机构:
动物营养与饲料安全湖北省协同创新中心动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院,湖北武汉430023;[丁斌鹰; 杨书慧; 廖满; 谭灵琳; 王蕾; 易丹] 武汉轻工大学
关键词:
α-酮戊二酸;热应激;肉鸡;肉品质;能量代谢
摘要:
试验旨在研究α-酮戊二酸对热应激肉鸡生长性能和肌肉品质的影响.通过给高温应激肉鸡饲喂含0.5% α-酮戊二酸(α-ketoglutarate,AKG)的日粮35 d,研究AKG对热应激肉鸡生长性能、肌肉品质、抗氧化功能和能量代谢的影响.结果表明:①与对照组相比,热应激处理显著降低了肉鸡(1~35日龄)的生长性能(P<0.05)和组织(胸腺、肝脏、胸肌和腹脂)的相对重量(P<0.05),同时也降低了肉鸡胸肌的a值、抗氧化酶(过氧化氢酶CAT和谷胱甘肽过氧化物酶GSH-Px)的活力、三磷酸腺苷(ATP)和二磷酸腺苷(ADP)含量及能荷(EC)值,但是提高了肉鸡胸肌一磷酸腺苷(AMP)的含量(P<0.05).②日粮中添加0.5%的AKG降低了热应激肉鸡的料重比(1~35日龄)、胸肌AMP含量(P<0.05),提高了热应激肉鸡血液抗氧化酶(CAT、SOD和GSH-Px)的活力(P<0.05),胸肌ATP、ADP和腺苷酸池(TAN)的含量及EC值(P<0.05).因此,日粮中添加0.5%的AKG可以降低热应激肉鸡的料重比,提高血液的抗氧化能力,改善胸肌的能量代谢.
语种:
中文
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