作者机构：
[Hou, Yongqing; Li, Peng; Guo, Chenyu; Tong, Wenfei; Han, Shaochen; Ding, Binying] Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources of Agricultural By-products, Ministry of Education,Wuhan Polytechnic University, Wuhan, Hubei 430023, PR China;[Sun, Xiangxue; Xiao, Lei] Hubei Lan Good microbial Technology Co., Ltd. Yichang, Hubei 443100, PR China;[Hu, Qunbing] Hubei Horwath Biotechnology Co., Ltd. Xianning, Hubei 437000, PR China;[Yi, Dan] Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources of Agricultural By-products, Ministry of Education,Wuhan Polytechnic University, Wuhan, Hubei 430023, PR China. Electronic address: yidan810204@163.com
通讯机构：
[Dan Yi] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources of Agricultural By-products, Ministry of Education,Wuhan Polytechnic University, Wuhan, Hubei 430023, China
摘要：
Farnesol ( FAN ), one of plant essential oils, is widely found in a variety of natural plants. Studies demonstrated that FAN contributed to the antioxidant and immune function as well as improving the intestinal flora, however effects of it on the broiler chickens has not been fully characterized. In the present study, we present an undated report of its effects on growth performance, antioxidant and immune functions of broiler chickens challenged with lipopolysaccharide ( LPS ). One hundred healthy male AA + broiler chickens with uniform body weight were divided into control and FAN groups, there were five replicates and 10 birds in each one. The trial lasted for 28 days, and two birds with uniform body weight were selected from each replicate to be treated with intraperitoneal injection of LPS at the end of the trial, and then samples were harvested after 3 h. Results showed that dietary supplementary with FAN tended to improve the feed conversion ratio ( FCR ) ( P = 0.058). The levels of serum lactate dehydrogenase and IL-1β were elevated in the birds challenged with LPS, as well as the content of malondialdehyde in the ileal and liver ( P < 0.05). Additionally, LPS treatment descended the levels of catalase and superoxide dismutase, and the ratio of villi height to crypt depth in the ileum ( P < 0.05). Dietary supplementation with FAN was able to alleviate the abnormal changes of the above indexes caused by LPS. In addition, dietary supplementation with FAN also contributed to alleviating the up-regulation of Toll-like receptor 4 ( TLR-4 ), nuclear transcription factor κB ( NF-κB ), myeloid differentiation primary response gene 88 ( MYD88 ), tumor necrosis factor ( TNF-α ) and IL-1β transcription levels in the ileum and liver of birds challenged with LPS ( P < 0.05). Results of intestinal flora demonstrated that the relative abundance of Candidatus Arthromitus was up-regulated in the ileal chyme of birds challenged with LPS, and dietary supplementation with FAN could reshape it. Intriguingly, the relative abundance of Candidatus Arthromitus was positively correlated with the mRNA levels of TLR-4, NF-κB, MYD88, TNF-α and IL-1β in the ileum ( P < 0.05). In conclusion, dietary supplementation with FAN might confer a protective effect on the intestine of broiler chickens challenged with lipopolysaccharide by reshaping intestinal flora, especially Candidatus Arthromitus, and regulating TLR4/NF-κB signaling pathway.
Farnesol ( FAN ), one of plant essential oils, is widely found in a variety of natural plants. Studies demonstrated that FAN contributed to the antioxidant and immune function as well as improving the intestinal flora, however effects of it on the broiler chickens has not been fully characterized. In the present study, we present an undated report of its effects on growth performance, antioxidant and immune functions of broiler chickens challenged with lipopolysaccharide ( LPS ). One hundred healthy male AA + broiler chickens with uniform body weight were divided into control and FAN groups, there were five replicates and 10 birds in each one. The trial lasted for 28 days, and two birds with uniform body weight were selected from each replicate to be treated with intraperitoneal injection of LPS at the end of the trial, and then samples were harvested after 3 h. Results showed that dietary supplementary with FAN tended to improve the feed conversion ratio ( FCR ) ( P = 0.058). The levels of serum lactate dehydrogenase and IL-1β were elevated in the birds challenged with LPS, as well as the content of malondialdehyde in the ileal and liver ( P < 0.05). Additionally, LPS treatment descended the levels of catalase and superoxide dismutase, and the ratio of villi height to crypt depth in the ileum ( P < 0.05). Dietary supplementation with FAN was able to alleviate the abnormal changes of the above indexes caused by LPS. In addition, dietary supplementation with FAN also contributed to alleviating the up-regulation of Toll-like receptor 4 ( TLR-4 ), nuclear transcription factor κB ( NF-κB ), myeloid differentiation primary response gene 88 ( MYD88 ), tumor necrosis factor ( TNF-α ) and IL-1β transcription levels in the ileum and liver of birds challenged with LPS ( P < 0.05). Results of intestinal flora demonstrated that the relative abundance of Candidatus Arthromitus was up-regulated in the ileal chyme of birds challenged with LPS, and dietary supplementation with FAN could reshape it. Intriguingly, the relative abundance of Candidatus Arthromitus was positively correlated with the mRNA levels of TLR-4, NF-κB, MYD88, TNF-α and IL-1β in the ileum ( P < 0.05). In conclusion, dietary supplementation with FAN might confer a protective effect on the intestine of broiler chickens challenged with lipopolysaccharide by reshaping intestinal flora, especially Candidatus Arthromitus, and regulating TLR4/NF-κB signaling pathway.
摘要：
The aim of this study was to investigate the effects of dietary l-glutamine (Gln) supplementation on the morphology and function of the intestine and the growth of muscle in piglets. In this study, sixteen 21-day-old piglets were randomly divided into two groups: the Control group (fed a basal diet) and the Gln group (fed a basal diet supplemented with 0.81% Gln). Blood, gut, and muscle samples were collected from all piglets on Day 20 of the trial. Compared with the Control group, the supplementation of Gln increased (p < 0.05) the villus height, villus width, villus surface area, and villus height/crypt depth ratio of the small intestine. Furthermore, the supplementation of Gln increased (p < 0.05) total protein, total protein/DNA, and RNA/DNA in both the jejunum and ileum. It also increased (p < 0.05) the concentrations of carnosine and citrulline in the jejunal mucosa, as well as citrulline and cysteine concentrations in the ileum. Conversely, Gln supplementation decreased (p < 0.05) Gln concentrations in both the jejunum and ileum, along with β-aminoisobutyric acid and 1-Methylhistidine concentrations, specifically in the ileum. Subsequent research revealed that Gln supplementation increased (p < 0.05) the mRNA levels for glutathione-S-transferase omega 2 and interferon-β in the duodenum. In addition, Gln supplementation led to an increase (p < 0.05) in the number of Lactobacillus genus in the colon, but a decrease (p < 0.05) in the level of HSP70 in the jejunum and the activity of diamine oxidase in plasma. Also, Gln supplementation reduced (p < 0.05) the mRNA levels of glutathione-S-transferase omega 2 and interferon stimulated genes, such as MX1, OAS1, IFIT1, IFIT2, IFIT3, and IFIT5 in both the jejunum and ileum, and the numbers of Clostridium coccoides, Enterococcus genus, and Enterobacterium family in the colon. Moreover, Gln supplementation enhanced (p < 0.05) the concentrations of total protein, RNA/DNA, and total protein/DNA ratio in the longissimus dorsi muscle, the concentrations of citrulline, ornithine, arginine, and hydroxyproline, and the mRNA level of peptide transporter 1, while reducing the contents of hydrogen peroxide and malondialdehyde and the mRNA level of glutathione-S-transferase omega 2 in the longissimus dorsi muscle. In conclusion, dietary Gln supplementation can improve the intestinal function of piglets and promote the growth of the longissimus dorsi muscle.
关键词：
glycerol monolaurate;cinnamaldehyde;intestinal morphology;cecal microbiota;laying hen
摘要：
This study was to determine the effects of the mixture of glycerol monolaurate and cinnamaldehyde ( GCM ) supplementation on the intestinal morphology, immunity, antioxidant status and cecal microbiota of laying hens. A total of 1,120 healthy laying hens (Jingfen-1 strain) at the age of 14 wk were randomly divided into 4 groups with 10 replicates of 28 layers in each and layers were fed diets containing 0 (control group), or 250, 500, and 1,000 mg/kg GCM for 12 wk. The results showed that dietary supplementation with GCM significantly increased intestinal villus height and villus height/crypt depth, duodenal villus area, total superoxide disumutase activities in the liver and jejunum, jejunal glutathione peroxidase activities while decreased duodenal and jejunal crypt depth, hydrogen peroxide content in the liver and jejunal malondialdehyde content of laying hens aging 28 wk ( P < 0.05). Meanwhile, GCM addition significantly increased serum immunoglobulin A and immunoglobulin M concentration of layers at the age of 20, 24, and 28 wk ( P < 0.05). Moreover, it was observed in the 16S rRNA sequencing that the addition of GCM elevated the abundance and diversity of gut microbiota in laying hens. The predominant bacteria from each group were Bacteroidota and Firmicutes at the phylum level and Bacteroides and Lactobacillus were the dominant genera. The composition and structure of cecal microflora were changed by the addition of GCM to the diet of laying hens. In conclusion, the addition of GCM (500–1,000 mg/kg diet) can improve intestinal morphology, immune function, intestinal and liver antioxidant status and intestinal flora of laying hens, thereby improving intestinal digestion and absorption capacity. These findings provide a new way to further explore the mechanism of GCM improving intestinal health.
This study was to determine the effects of the mixture of glycerol monolaurate and cinnamaldehyde ( GCM ) supplementation on the intestinal morphology, immunity, antioxidant status and cecal microbiota of laying hens. A total of 1,120 healthy laying hens (Jingfen-1 strain) at the age of 14 wk were randomly divided into 4 groups with 10 replicates of 28 layers in each and layers were fed diets containing 0 (control group), or 250, 500, and 1,000 mg/kg GCM for 12 wk. The results showed that dietary supplementation with GCM significantly increased intestinal villus height and villus height/crypt depth, duodenal villus area, total superoxide disumutase activities in the liver and jejunum, jejunal glutathione peroxidase activities while decreased duodenal and jejunal crypt depth, hydrogen peroxide content in the liver and jejunal malondialdehyde content of laying hens aging 28 wk ( P < 0.05). Meanwhile, GCM addition significantly increased serum immunoglobulin A and immunoglobulin M concentration of layers at the age of 20, 24, and 28 wk ( P < 0.05). Moreover, it was observed in the 16S rRNA sequencing that the addition of GCM elevated the abundance and diversity of gut microbiota in laying hens. The predominant bacteria from each group were Bacteroidota and Firmicutes at the phylum level and Bacteroides and Lactobacillus were the dominant genera. The composition and structure of cecal microflora were changed by the addition of GCM to the diet of laying hens. In conclusion, the addition of GCM (500–1,000 mg/kg diet) can improve intestinal morphology, immune function, intestinal and liver antioxidant status and intestinal flora of laying hens, thereby improving intestinal digestion and absorption capacity. These findings provide a new way to further explore the mechanism of GCM improving intestinal health.
摘要：
The purpose of this study was to determine the efficacy of tannic acid on the antioxidative function, immunity, and intestinal barrier of broilers co-infected with coccidia and Clostridium perfringens (CCP). A total of 294 1-day-old arbor acres(AA) broilers were divided into three groups: control group (CON), CCP co-infected group (CCP), and 1000 mg/kg TA + CCP co-infected group (CTA). This trial lasted for 28 days. The results showed that the CCP group decreased the activity of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), catalase (CAT), and total antioxidant capacity (T-AOC) levels and increased the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the jejunum (p < 0.05). The mRNA levels of GSH-Px3 and CAT in the liver and jejunum, and the mRNA levels of GSH-Px3, SOD, HO-1, and NAD(P)H quinone oxidoreductase I (NQO1) in the liver were down-regulated by CCP challenge (p < 0.05). In addition, the Keap1 and Nrf2 mRNA levels in the liver and jejunum, jejunal glutathione S-transferase (GST), and heme-oxygenase-1 (HO-1) were upregulated in the CCP group compared with CON (p < 0.05). The mRNA levels of interleukin 8 (IL-8), IL-1β, inducible nitric oxide synthase (iNOS), and interferon γ (IFN-γ) in the jejunum were elevated, and jejunal mRNA levels of IL-10, zonula occludens protein1 (ZO-1), claudin-1, claudin-2, and occludin were decreased in the CCP treatment (p < 0.05). Dietary supplementation with 1000 mg/kg TA increased the activity of GSH-Px, T-SOD, CAT, and T-AOC and decreased the contents of H2O2 and MDA in the jejunum (p < 0.05). Compared with the CCP group, TA decreased the mRNA level of Keap1 and Nrf2 in the liver and jejunum, increased the GSH-Px3, SOD, and CAT mRNA in the liver, and alleviated the rise of IL-8, IL-1β, iNOS, and IFN-γ and decrease in IL-10, occludin gene expression in the jejunum (p < 0.05). In conclusion, the addition of 1000 mg/kg TA to the diet improved the jejunal barrier, mitigated the jejunal inflammation, and increased the antioxidant capacity of the liver and jejunum through the activation of the transcription factor Nrf2 downstream of the Nrf2-Keap1 pathway in broilers with NE condition.
作者机构：
[张正帆; 徐朋涛; 侯永清; 何来; 刘志鹏; 王思甜; 刘诚傲; 齐雅; 丁斌鹰; 郑丽云; 郭双双] Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China;[童庆芳] Wuhan Jiangxia District Animal Disease Prevention and Control Center, Wuhan, 430072, China
通讯机构：
[Zhang, Z.] E;Engineering Research Center of Feed Protein Resources on Agricultural By-Products, China
作者机构：
[Guo, S. S.; Liu, Z. P.; Li, L. L.; Xu, P. T.; Ding, B. Y.; Chao, J. R.; Zhang, Z. F.] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Peoples R China.;[Lv, H. Y.] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Lv, H. Y.] Beijing Ctr Biol Co Ltd, Beijing 102600, Peoples R China.
通讯机构：
[L.L. Li; S.S. Guo] E;Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China
关键词：
Cnicus japonicus;Lonicera flos;antioxidant status;inflammatory cytokine;laying hen
摘要：
This study was conducted to investigate the effects of Lonicera flos and Cnicus japonicus extracts ( LCE ) on the laying performance, egg quality, morphology, antioxidant status, inflammatory-related cytokines, and shell matrix protein expression of oviduct in laying hens. A total of 1,728 Roman Pink laying hens aged 73-wk-old were randomly assigned into 4 groups (18 replicates/group, 24 layers/replicate) fed basal diets supplemented with 0, 300, 500, and 1,000 mg of LCE per kg of diet, respectively. The trial lasted for 11 wk, including 2-wk adjustment period and 9-wk testing period. The results indicated that laying hens fed diets supplemented with LCE linearly increased egg weight, yolk color and shell thickness at wk 78 and albumen height, Haugh unit and shell thickness at wk 83 ( P < 0.05). At wk 78, LCE groups linearly affected the hydrogen peroxide content in magnum ( P < 0.05) and 300 mg/kg LCE groups had the highest catalase activity in isthmus ( P < 0.05). At wk 83, LCE groups linearly reduced ( P < 0.05) hydrogen peroxide content in the magnum and isthmus and malondialdehyde content in the uterus whereas increased catalase activity in isthmus ( P < 0.05). Furthermore, LCE levels quadratically affected glutathione peroxidase activity in isthmus at wk 83 ( P < 0.05). At wk 78, the mRNA expressions of inducible nitric oxide synthase and interferon-γ in isthmus and ovalbumin and ovocleidin-116 in uterus had linear effects in response to LCE levels ( P < 0.05) and 1,000 mg/kg LCE group had the lowest mRNA expression of interleukin-6 in magnum ( P < 0.05). At wk 83, LCE supplementation linearly decreased the mRNA expression of interleukin-1β, interferon-γ and tumor necrosis factor-α in magnum and tumor necrosis factor-α and inducible nitric oxide synthase in uterus ( P < 0.05). It is concluded that LCE improved egg quality partly by modulating antioxidant status, inflammatory-related cytokines and shell matrix protein expression of oviduct in laying hens.
This study was conducted to investigate the effects of Lonicera flos and Cnicus japonicus extracts ( LCE ) on the laying performance, egg quality, morphology, antioxidant status, inflammatory-related cytokines, and shell matrix protein expression of oviduct in laying hens. A total of 1,728 Roman Pink laying hens aged 73-wk-old were randomly assigned into 4 groups (18 replicates/group, 24 layers/replicate) fed basal diets supplemented with 0, 300, 500, and 1,000 mg of LCE per kg of diet, respectively. The trial lasted for 11 wk, including 2-wk adjustment period and 9-wk testing period. The results indicated that laying hens fed diets supplemented with LCE linearly increased egg weight, yolk color and shell thickness at wk 78 and albumen height, Haugh unit and shell thickness at wk 83 ( P < 0.05). At wk 78, LCE groups linearly affected the hydrogen peroxide content in magnum ( P < 0.05) and 300 mg/kg LCE groups had the highest catalase activity in isthmus ( P < 0.05). At wk 83, LCE groups linearly reduced ( P < 0.05) hydrogen peroxide content in the magnum and isthmus and malondialdehyde content in the uterus whereas increased catalase activity in isthmus ( P < 0.05). Furthermore, LCE levels quadratically affected glutathione peroxidase activity in isthmus at wk 83 ( P < 0.05). At wk 78, the mRNA expressions of inducible nitric oxide synthase and interferon-γ in isthmus and ovalbumin and ovocleidin-116 in uterus had linear effects in response to LCE levels ( P < 0.05) and 1,000 mg/kg LCE group had the lowest mRNA expression of interleukin-6 in magnum ( P < 0.05). At wk 83, LCE supplementation linearly decreased the mRNA expression of interleukin-1β, interferon-γ and tumor necrosis factor-α in magnum and tumor necrosis factor-α and inducible nitric oxide synthase in uterus ( P < 0.05). It is concluded that LCE improved egg quality partly by modulating antioxidant status, inflammatory-related cytokines and shell matrix protein expression of oviduct in laying hens.
摘要：
This study was conducted to investigate effects of dietary Limosilactobacillus fermentum and Lacticaseibacillus paracasei supplementation on the intestinal stem cell proliferation, immunity, and ileal microbiota of broiler chickens challenged by coccidia and Clostridium perfringens. A total of 336 one-day-old Ross 308 chickens were randomly assigned into four groups. Chickens in the control (CTR) group were fed basal diet, and chickens in the three challenged groups were fed basal diets supplemented with nothing (CCP group), 1.0 × 10(9) CFU/kg L. fermentum (LF_CCP group), and 1.0 × 10(9) CFU/kg L. paracasei (LP_CCP group), respectively. All challenged birds were infected with coccildia on day 9 and Clostridium perfringens during days 13-18. The serum and intestinal samples were collected on days 13 and 19. The results showed that L. fermentum significantly increased jejunal gene expression of cdxB (one of the intestinal stem cell marker genes) on day 13. Additionally, L. fermentum significantly up-regulated mRNA levels of JAK3 and TYK2 and tended to increase STAT6 mRNA expression in jejunum on day 19. In the cecal tonsil, both L. fermentum and L. paracasei decreased mRNA expression of JAK2 on day 13, and L. fermentum down-regulated JAK1-2, STAT1, and STAT5-6 gene expressions on day 19. Ileal microbiological analysis showed that coccidial infection increased the Escherichia-Shigella, Lactobacillus, and Romboutsia abundance and decreased Candidatus_Arthromitus richness on day 13, which were reversed by Lactobacillus intervention. Moreover, Lactobacilli increased ileal Lactobacillus richness on day 19. In conclusion, Lactobacilli alleviated the impairment of intestinal stem cell proliferation and immunity in coccidia- and C. perfringens-challenged birds via modulating JAK/STAT signaling and reshaping intestinal microflora.
摘要：
Simple Summary Plant extracts are one of the alternatives to antibiotics, and are generally considered to be safe for animals and effective against pathogens. Their antimicrobial, anti-inflammatory, antioxidant, and antiviral activities have been well documented. Puerarin, the main component of pueraria extract, is a C-glucoside of isoflavone daidzein. Curcumin, isolated from the rhizome of curcuma, is a kind of polyphenol. The current study was carried out to examine the synergistic effects of pueraria extract and curcumin on the growth performance, antioxidant capacity and intestinal barriers of broilers. Our observations showed that supplementation of pueraria extract and curcumin alone or in combination did not improve the growth performance of broilers in the 28-day trial, but enhanced the antioxidant status and intestinal integrity of broilers by increasing the activities of antioxidant enzymes and promoting intestinal morphology. Pueraria extract and curcumin are potential modulators of antioxidant function and intestinal health. Their beneficial effects on improving growth performance need further investigation. The current study was carried out to examine the effects of pueraria extract (PE) and curcumin (CUR) on growth performance, antioxidant capacity and intestinal integrity in broiler chickens. A complete randomized design with a 2 x 2 factorial arrangement of treatments was employed to assign 200 one-day-old Ross-308 broilers to four groups, each including five replicates of ten birds. Chickens in the control group (CON) were fed the basal diet, while the PE, CUR and PE+CUR groups were supplemented with 200 mg/kg PE or 200 mg/kg CUR or 200 mg/kg PE+ 200 mg/kg CUR. This trial lasted for 28 days. The PE supplementation decreased the average daily gain during the whole period (p < 0.05). The PE+CUR group had a higher feed conversion ratio than that of the PE and CUR groups during days 14-28 and 1-28 (p < 0.05). Dietary CUR supplementation increased duodenal T-SOD activity (p < 0.05). Compared with the CON group, the other three groups increased the duodenal GSH-Px activity, the PE+CUR group reduced the duodenal H2O2 level, and the CUR and PE groups elevated the ileal GSH-Px activity and the ratio of jejunal villus height to crypt depth, respectively (p < 0.05). The addition of PE decreased crypt depth and increased villus area and mucin-2 mRNA level in the jejunum (p < 0.05). Overall, dietary supplementation with PE, CUR, or a combination of these, enhanced the antioxidant status and intestinal integrity of broilers.
作者机构：
[Guo, S. S.; Li, L. L.; Liu, Z. P.; Xu, P. T.; Ding, B. Y.; Zhang, Z. F.; Liu, C. A.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Dong, X. Y.] Zhejiang Univ, Key Lab Anim Feed & Nutr Zhejiang Prov, Hangzhou 310058, Peoples R China.
关键词：
salpingitis;lipopolysaccharide;inflammatory-related cytokines;histomorphometry;laying hen
摘要：
This study was conducted to simulate salpingitis of laying hens by observing the morphology and expression of inflammatory genes in the oviduct. A total of one hundred twenty 81-wk-old Roman Pink laying hens in good physical condition without the oviduct disease with an average egg production rate of 76% were fed a basal diet for 2 wks and then randomly allocated into 4 groups (6 replicates/group, 5 birds/replicate). The experimental treatments were as follows: 1) Control group (treated with PBS); 2) Organic chemical reagent ( OCR ) group; 3) Lipopolysaccharide ( LPS ) group; 4) LPS + OCR group. First, the chickens were kept upside down to make ectropion and exposure of the apertura uterinae; then prepared reagents were poured into the uterine part of the fallopian tube by using the chicken vas deferens (1 mL/layer); finally, the chickens were kept in the inverted position for 5 to 10 min. The fallopian tube samples (the magnum, isthmus, and uterus) were collected after 48 h of treatment. Compared with the control, treatment with LPS+OCR decreased ( P < 0.05) the secondary villus length and primary villus area in magnum and villus length in isthmus ( P < 0.05). An increase ( P < 0.05) of the intervillous space of uterus was observed in LPS + OCR group compared with the control. The expressions of interleukin-6 mRNA of magnum and interferon-γ ( IFN-γ ) of isthmus in the LPS and LPS+OCR treatments were higher ( P < 0.05) than that in control. Compared with the control, treatment with LPS+OCR increased ( P < 0.05) the expressions of IFN-γ mRNA of magnum and IFN-γ, tumor necrosis factor-α and inducible nitric oxide synthase mRNA of uterus in laying hens. In conclusion, the results of morphological damage of fallopian tube tissue and increased expression of inflammatory factors in LPS + OCR treatment group suggested that LPS+OCR treatment can provide data basis to establish salpingitis model in laying hens for studying the pathogenesis of it.
This study was conducted to simulate salpingitis of laying hens by observing the morphology and expression of inflammatory genes in the oviduct. A total of one hundred twenty 81-wk-old Roman Pink laying hens in good physical condition without the oviduct disease with an average egg production rate of 76% were fed a basal diet for 2 wks and then randomly allocated into 4 groups (6 replicates/group, 5 birds/replicate). The experimental treatments were as follows: 1) Control group (treated with PBS); 2) Organic chemical reagent ( OCR ) group; 3) Lipopolysaccharide ( LPS ) group; 4) LPS + OCR group. First, the chickens were kept upside down to make ectropion and exposure of the apertura uterinae; then prepared reagents were poured into the uterine part of the fallopian tube by using the chicken vas deferens (1 mL/layer); finally, the chickens were kept in the inverted position for 5 to 10 min. The fallopian tube samples (the magnum, isthmus, and uterus) were collected after 48 h of treatment. Compared with the control, treatment with LPS+OCR decreased ( P < 0.05) the secondary villus length and primary villus area in magnum and villus length in isthmus ( P < 0.05). An increase ( P < 0.05) of the intervillous space of uterus was observed in LPS + OCR group compared with the control. The expressions of interleukin-6 mRNA of magnum and interferon-γ ( IFN-γ ) of isthmus in the LPS and LPS+OCR treatments were higher ( P < 0.05) than that in control. Compared with the control, treatment with LPS+OCR increased ( P < 0.05) the expressions of IFN-γ mRNA of magnum and IFN-γ, tumor necrosis factor-α and inducible nitric oxide synthase mRNA of uterus in laying hens. In conclusion, the results of morphological damage of fallopian tube tissue and increased expression of inflammatory factors in LPS + OCR treatment group suggested that LPS+OCR treatment can provide data basis to establish salpingitis model in laying hens for studying the pathogenesis of it.
作者：
Zhang, Q. I. A. N.;Zhang, L. I. N.;Du, L. I. N. X. I. A. O.;Zhang, Y. A. N. Y. A. N.;Yi, D. A. N.;...
期刊：
CZECH JOURNAL OF ANIMAL SCIENCE,2023年68(7):296-305 ISSN：1212-1819
通讯作者：
Wu, T
作者机构：
[Ding, B. I. N. Y. I. N. G.; Du, L. I. N. X. I. A. O.; Yi, D. A. N.; Wu, T; Zhang, Y. A. N. Y. A. N.; Zhao, D., I; Wu, T. A. O.; Zhang, Q. I. A. N.; Hou, Y. O. N. G. Q. I. N. G.] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Prod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan, Peoples R China.;[Zhang, L. I. N.] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wu, T ] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Prod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan, Peoples R China.
