合生元对生长育肥猪生长性能、胴体品质和肉品质的影响
作者：
罗鹏;李爱科;任慧慧;何贝贝;王丽;...
期刊：
动物营养学报 ,2021年33(10):5908-5916 ISSN：1006-267X
作者机构：
[丁斌鹰; 罗鹏] 武汉轻工大学动物科学与营养工程学院;[成俊林; 王薇薇; 李爱科; 王丽; 何贝贝] 国家粮食和物资储备局科学研究院;[任慧慧] 青岛康尔生物工程有限公司
关键词：
合生元;胴体品质;肉品质;生长育肥猪
摘要：
本试验旨在研究由植物乳杆菌、屎肠球菌和壳寡糖组成的合生元(SYN)对生长育肥猪生长性能、胴体品质、肉品质和肉质感官评定的影响。试验选用90头(50±2)日龄、体重[(33± 6) kg]相近、窝龄相近、健康状况良好的“杜×长×大”三元杂交生长猪,根据体重随机分为3个组,每组6个重复(公母各占1 / 2,其中公猪为去势公猪),每个重复5头猪。3个组分别为:1)对照组(CON组),饲喂基础饲粮;2)抗生素组(ANT组),生长期阶段(30 ~ 60 kg)饲喂基础饲粮+ 0.002%维吉尼亚霉素,育肥期阶段(61 kg至出栏)饲喂基础饲粮;3)合生元组(SYN组),饲喂基础饲粮+合生元(0.05%植物乳杆菌+0.05%屎肠球菌+0.05%壳寡糖) 。试验预试期7 d,正试期120 d。结果表明:1)与CON组相比,ANT组和SYN组公猪或母猪的生长性能(包括平均日增重、平均日采食量和料重比)均无显著差异(P >0.05),但死亡率有所降低。2)与CON组相比,ANT和SYN组生长育肥猪的胴体品质(包括屠宰率、胴体长、背膘厚、眼肌面积和腿臀比例等)均无显著差异(P>0.05),但背膘厚有降低趋势(0.05≤P<0.10) 。3) SYN组生长育肥猪背最长肌剪切力显著低于CON组(P<0.05),与ANT组差异不显著(P>0.05),即饲粮添加合生元显著改善了猪肉的嫩度。各组其他肉品质指标(pH45 min 、滴水损失、宰后45 min和24 h的肉色以及大理石纹评分等)之间无显著差异(P>0.05) 。4)猪肉感官评定中,SYN组里脊肉嫩度显著高于ANT组(P<0.05),后腿肉嫩度显著高于CON组(P<0.05) 。由此可见,饲粮中添加由植物乳杆菌、屎肠球菌和壳寡糖组成的合生元,虽然对30 kg至出栏阶段生长育肥猪的生长性能无显著影响,但是有降低生长育肥猪背膘厚的趋势,并且可显著改善猪肉嫩度、降低死亡率。
语种：
中文
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发酵乳杆菌和凝结芽孢杆菌对产气荚膜梭菌感染肉鸡生长性能和肠道健康的影响
作者：
夏亿;张元可;徐晶云;郭双双;丁斌鹰
期刊：
中国畜牧兽医 ,2019年46(10):2927-2936 ISSN：1671-7236
作者机构：
[徐晶云; 丁斌鹰; 郭双双; 张元可; 夏亿] 武汉轻工大学
关键词：
产气荚膜梭菌;肉鸡;发酵乳杆菌;凝结芽孢杆菌;肠道健康
摘要：
试验以产气荚膜梭菌感染肉仔鸡建立坏死性肠炎模型,研究发酵乳杆菌和凝结芽孢杆菌对感染肉鸡生长性能和肠道健康的影响。将336只1日龄爱拔益加肉仔鸡随机分成4个处理组,每个处理6个重复。4个处理组分别为对照组、感染组、感染+LF组(日粮添加1×10^9 CFU/kg发酵乳杆菌)、感染+BC组(日粮添加1×10^10 CFU/kg凝结芽孢杆菌)。所有感染肉鸡在14~21 d经口接种A型产气荚膜梭菌。试验期为28 d。结果显示:与对照组相比,灌服产气荚膜梭菌显著降低22~28 d肉鸡的日均采食量(P<0.05),显著增加28 d十二指肠和空肠损伤评分(P<0.05),显著提高21 d肉鸡盲肠的产气荚膜梭菌和大肠杆菌数量,显著降低28 d回肠产气荚膜梭菌数量(P<0.05)。与感染组相比,日粮添加发酵乳杆菌显著提高21 d肉鸡回肠的乳杆菌属数量(P<0.05),显著降低21 d盲肠大肠杆菌的数量(P<0.05);日粮添加凝结芽孢杆菌显著降低28 d肉鸡十二指肠损伤评分(P<0.05),显著降低21 d十二指肠隐窝深度(P<0.05),显著提高21 d空肠绒毛高度与隐窝深度的比值(P<0.05),显著降低21 d肉鸡回肠和盲肠的大肠杆菌数量(P<0.05),显著提高28 d肉鸡回肠乳杆菌属数量(P<0.05)。综上所述,日粮中添加发酵乳杆菌或凝结芽孢杆菌均对感染肉鸡的肠道微生物具有调控作用;其中添加凝结芽孢杆菌有利于肠道绒毛发育,缓解肠道损伤,一定程度上提高了肉鸡的生长性能,而发酵乳杆菌没有缓解肠道损伤的作用。
语种：
中文
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几种蛋白质饲料原料和配合饲料氨基酸组成的调查分析
作者：
张元可;程强;郭双双;丁斌鹰;严义梅
期刊：
饲料研究 ,2019年42(1):61-65 ISSN：1002-2813
作者机构：
武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心;武汉轻工大学动物营养与饲料科学湖北省重点实验室;武汉轻工大学农业部饲料资源与加工科学观测实验站;[严义梅] 湖北国正质量技术服务有限公司;[丁斌鹰; 程强; 郭双双; 张元可] 武汉轻工大学
关键词：
蛋白质饲料原料;配合饲料;氨基酸
摘要：
饲料中氨基酸组成及其稳定性是评定蛋白质饲料原料和配合饲料蛋白质品质的重要指标.调查收集来自湖北省及周边各地样品(鱼粉211份、肉粉121份、DDGS 22份和仔猪前期配合料37份、混养鱼料133份),分析测定了17种氨基酸(AA)含量,并将饲料原料中限制性氨基酸与中国饲料数据库中数据进行比对.调查结果显示,鱼粉AA含量合格率达到100％,肉粉AA含量合格率为86.78％,DDGS的AA含量合格率较差.混养鱼料中赖氨酸水平(1.77±0.51)％,占鱼料总氨基酸(8.5±0.5)％;仔猪前期配合料赖氨酸水平(1.12±0.39)％,占仔猪前期配合料总氨基酸(7.0±1.0)％.调查结果表明,被检的鱼粉和肉粉具有较高的AA合格率.
语种：
中文
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Generation of epidermal growth factor and green fluorescent protein coexpressing Lactobacillus acidophilus and its effect on intestinal histology improvement in mature LPS-challenged mice
作者：
Lv, Yang;Kang, Ping;Zhang, Lin;Li, Xueni;Wang, Lei;...
期刊：
THAI JOURNAL OF VETERINARY MEDICINE ,2018年48(2):147-156 ISSN：0125-6491
通讯作者：
Wu, Tao
作者机构：
[Wu, Tao; Lv, Yang; Kang, Ping; Hou, Yongqing; Wang, Lei; Li, Xueni; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.
通讯机构：
[Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Epidermal growth factor;Intestinal histology;LPS-challenged mice;Lactobacillus acidophilus
摘要：
The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named ΔMG6243-1 and ΔMG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 μl MG6243, 4) LPS-challenged + 200 μl ΔMG6243-1, 5) LPS-challenged + 200 μl ΔMG6243-1 + inducer IPTG, and 6) LPS-challenged + 200 μl ΔMG6243-2. There were ten replicates for each treatment, and each replicate had one mouse. On d 15, the LPS-challenged mice were injected intraperitoneally with LPS at 10 mg/kg BW, while the non-challenged mice were injected with 0.9% NaCl solution. Three hours after the challenge, all mice were killed. Results showed that both ΔMG6243-1 and ΔMG6243-2 could increase villus height in the duodenum, ileum and jejunum (P < 0.05). In addition, the recombinant L. acidophilus decreased the crypt depth in the duodenum and ileum (P < 0.05). These results indicated that the recombinant L. acidophilus could effectively improve the intestinal histology in LPS-challenged mice. © 2018 Chulalongkorn University Printing House.
语种：
英文
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Dietary Supplementation with Trihexanoin Enhances Intestinal Function of Weaned Piglets
作者：
Wu, Tao;Li, Kang;Yi, Dan;Wang, Lei;Zhao, Di;...
期刊：
International Journal of Molecular Sciences ,2018年19(10):3277- ISSN：1661-6596
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Li, Kang; Lv, Yang; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.
关键词：
intestinal morphology and function;glycogen and fat metabolism;trihexanoin;weaned piglets
摘要：
Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: The control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate; increased the concentrations of LDL, HDL and total protein in plasma; decreased cholesterol concentrations and glutamyl transpeptidase activity in plasma; improved intestinal morphologic structure; altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism, mucosal barrier function, antioxidant capacity and water transport capacity; and altered the community of intestinal microflora. These results indicate that dietary trihexanoin supplementation could reduce diarrhea, regulate carbohydrate and fat metabolism, exert beneficial effects on the intestinal mucosal barrier, protect the intestinal mucosa from injuries, improve intestinal transport and absorption, and enhance antioxidant capacity. In conclusion, dietary supplementation with 0.5% trihexanoin improves the intestinal function and health of weaned piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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无抗无锌乳仔猪饲料技术研讨
作者：
丁斌鹰
作者机构：
武汉轻工大学动物科学与营养工程学院
会议名称：
2018年第七届国内外教槽料发展高峰论坛
会议时间：
2018-06-28
会议地点：
南京
会议论文集名称：
2018年第七届国内外教槽料发展高峰论坛论文集
关键词：
饲料添加剂;发酵溶菌酶;肠道健康;氧化锌
摘要：
发酵溶菌酶是一种广谱抗菌蛋白，也是一种无残留、无耐药性、可替代抗生素的绿色添加剂，可以保障动物肠道健康，有效预防仔猪腹泻，同时可以消除氧化锌的副作用，显著提高动物的健康水平和经济效益，是氧化锌最好的替代品。
语种：
中文
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Establishment of a porcine model of indomethacin-induced intestinal injury
作者：
Yi, Dan;Liu, Wenkai;Hou, Yongqing* ;Wang, Lei;Zhao, Di;...
期刊：
FRONTIERS IN BIOSCIENCE-LANDMARK ,2018年23(11):2166-2176 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Wenkai; Wu, Tao; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Animal Model;Hematology;Indomethacin;Inflammation;Intestinal injury;Piglets;Review
摘要：
A useful animal model of intestinal injury is pivotal for studying its pathogenesis and developing nutritional interventions (e.g., amino acid supplementation). Here, we propose the use of indomethacin (IDMT) to induce intestinal inflammation in neonatal pigs. Fourteen-day-old piglets fed a milk replacer diet receive intraperitoneal administration of IDMT (5 mg/kg body weight) for 3 consecutive days. On day 4, blood and intestinal samples are obtained for physiological and biochemical analyses. IDMT increases blood DAO activity, I-FABP concentration, neutrophil and eosinophil numbers; intestinal MMP3 mRNA levels, MPO activity, and MDA concentration; but reduces the plasma concentration of citrulline (synthesized exclusively by enterocytes of the small intestine), intestinal GSH-Px activity, and mRNA levels for villin, I-FABP, TRPV6, AQP10, and KCNJ13. Moreover, extensive hemorrhagic spots, thinned intestinal wall, and ulcers in the distal jejunum and ileum are observed in IDMT-challenged piglets. Furthermore, IDMT decreases intestinal villus height and villus surface area in the piglet jejunum. Collectively, this work establishes a porcine model of intestinal injury for designing novel nutritional means to improve gut function in pigs and humans. © 2018 Frontiers in Bioscience. All Rights Reserved.
语种：
英文
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益生菌对维生素K缺乏肉仔鸡骨骼健康的改善作用
作者：
李叶涵;方山青;毕云天;冯娟;杨阳;...
期刊：
中国家禽 ,2018年40(14):25-31 ISSN：1004-6364
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[毕云天; 丁斌鹰; 张岩岩; 郭双双; 冯娟; 杨阳; 方山青; 李叶涵] 武汉轻工大学
关键词：
枯草芽孢杆菌;凝结芽孢杆菌;维生素K;肉鸡;骨骼健康
摘要：
试验旨在研究益生菌对维生素K（V（k））缺乏肉鸡骨骼健康的改善作用。试验选用360只爱拔益加肉仔鸡,采用3×2因子试验设计,分成6个处理组,每组3个重复。饲喂日粮中不添加益生菌或者添加500 g/t的枯草芽孢杆菌（BS）或凝结芽孢杆菌（BC）,公、母鸡分栏饲养。所有处理组的日粮均不额外添加V_（ko）结果显示：在0~42日龄,添加BS和BC均有增加肉鸡日增重（P=0.078）和降低料重比（P=0.079）的趋势。添加BS增加了21日龄肉鸡的股骨和胫骨重以及42日龄胫骨重,两种益生菌均增加21日龄胫骨强度和钙量（P〈0.05）。益生菌和性别对21日龄胫骨的灰分含量以及42日龄胫骨长度、灰分含量、钙量和磷量存在互作效应（P〈0.05）,对21日龄血清中骨源性碱性磷酸酶（BALP）的含量也存在互作效应,饲喂BC的公鸡血清中BALP含量最高（P〈0.05）。与对照组相比,两种益生菌均增加了21日龄血浆中降钙素的水平,降低了42日龄血浆中甲状旁腺激素的含量（P〈0.05）。综上所述,添加BS和BC通过调节钙磷代谢增加了V（k）缺乏肉鸡胫骨的灰分和钙磷沉积,促进了骨骼的发育,从而提高了肉鸡生长性能。
语种：
中文
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Dietary supplementation with an amino acid blend enhances intestinal function in piglets
作者：
Yi, Dan;Li, Baocheng;Hou, Yongqing* ;Wang, Lei;Zhao, Di;...
期刊：
Amino Acids ,2018年50(8):1089-1100 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Li, Baocheng; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Zhou, Ying; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Amino acid blend;Intestinal function;Piglets
摘要：
The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB. On day 20 of the trial, blood and intestinal tissue samples were obtained from piglets. Compared with the control, AAB supplementation reduced (P < 0.05) diarrhoea incidence; plasma alanine aminotransferase and diamine oxidase activities; intestinal concentrations of hydrogen peroxide, malondialdehyde, and heat shock protein-70, and intestinal mRNA levels for interleukin-1β, interferon-γ, and chemokine (C–X–C motif) ligand-9; and the numbers of Enterobacterium family, Enterococcus genus and Clostridium coccoides in the colon digesta. Furthermore, AAB supplementation enhanced (P < 0.05): the plasma concentrations of serine, aspartate, glutamate, cysteine, tyrosine, phenylalanine, tryptophan, lysine, arginine, citrulline, ornithine, taurine, and γ-aminobutyric acid; intestinal villus height and surface area, villus height/crypt depth ratio, antioxidative enzyme activities, and mRNA levels for porcine β-defensin-1, sodium-independent amino acid transporters (b 0,+ AT and y + LAT1), aquaporin (AQP) 3, AQP8, AQP10, nuclear factor erythroid 2-related factor 2 and glutathione S-transferase omega-2, and protein abundances of AQP3, AQP4, claudin-1, occludin and myxovirus resistance 1; and the numbers of Bifidobacterium genus and Lactobacillus genus in the colon digesta. Collectively, these comprehensive results indicate that dietary AAB supplementation plays an important role in improving piglet growth and intestinal function. © 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
语种：
英文
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N-乙酰半胱氨酸和冷应激对肉鸡肠道组织形态、能量代谢和抗氧化能力的影响
作者：
王惠云;廖满;王蕾;赵迪;易丹;...
期刊：
中国畜牧杂志 ,2018年54(2):52-59 ISSN：0258-7033
作者机构：
动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉 430023;[丁斌鹰; 廖满; 王惠云; 王蕾; 刘玉兰; 易丹; 赵迪] 武汉轻工大学
关键词：
N-乙酰半胱氨酸;肉鸡;冷应激;肠道功能
摘要：
本试验旨在研究N-乙酰半胱氨酸(NAC)和冷应激对肉鸡肠道组织形态、能量状况和抗氧化能力的影响.试验将200只1日龄健康雄性科宝肉鸡随机分为4个处理组:对照组(基础日粮)、NAC组(基础日粮+0.1％NAC)、冷应激组(基础日粮)与冷应激+NAC组(基础日粮+0.1％NAC),每组5个重复,每个重复10只鸡.试验第1～2周按常规饲养方法控制温度,试验第3～6周冷应激组和冷应激+NAC组的温度控制在(10±2)℃,而对照组与NAC组维持在(26±2)℃.结果表明:冷应激处理使肉鸡平均日采食量、平均日增重、空肠低氧诱导因子1 (HIF-1) mRNA水平显著下降(P＜0.05),饲料转化率、回肠一磷酸腺苷(AMP)含量和空肠隐窝深度极显著升高(P＜0.01);日粮添加0.1％ NAC显著提高了冷应激肉鸡肠道绒毛高度(VH)、十二指肠与空肠绒毛高度与隐窝深度比值(VH/CD)、空肠三磷酸腺苷(ATP)和腺苷酸池(TAN)含量、回肠ATP和二磷酸腺苷(ADP)及能荷(AEC)含量、十二指肠超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力、回肠谷胱甘肽过氧化物酶(GSH-Px)活力、空肠血红素加氧酶(HMOX) mRNA水平(P＜0.05).因此,日粮中添加0.1％的N-乙酰半胱氨酸可改善肠道组织形态和能量状况,提高冷应激肉鸡肠道抗氧化能力.
语种：
中文
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Effects of dietary coated-oleum cinnamomi supplementation on the immunity and intestinal integrity of broiler chickens
作者：
Guo, Shuangshuang;Cheng, Qiang;Li, Yehan;Duan, Rui;Hou, Yongqing;...
期刊：
Animal Science Journal ,2018年89(11):1581-1590 ISSN：1344-3941
通讯作者：
Ding, Binying
作者机构：
[Cheng, Qiang; Duan, Rui; Guo, Shuangshuang; Li, Yehan; Hou, Yongqing; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Ding, Binying] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
broiler chicken;coated oleum cinnamomi;immunity;intestinal integrity
摘要：
Oleum cinnamomi (OCM) is increasingly used as a feed additive in animal diets. The aim of this study was to investigate the effects of dietary supplementation with coated‐OCM (cOCM) on the immunity and intestinal integrity of broiler chickens. A total of 396 one‐day‐old chicks were randomly assigned into six groups. The basal diets were supplemented with 50 mg/kg of flavomycin (positive control) as well as 0 (control), 50, 100, 200, and 300 mg/kg of cOCM. Compared with the control, both positive control and cOCM treatments did not improve the growth performance. Serum immunoglobulin (Ig) Y levels were decreased by flavomycin and 50 mg/kg of cOCM treatments (p < 0.05). Dietary cOCM decreased ileal secretory IgA contents at d 21 and commonly down‐regulated duodenal and ileal mRNA expression of interleukin (IL)‐1β and IL‐8 at d 42 (p < 0.05). The 300 mg/kg of cOCM increased jejunal ratio of villus height to crypt depth and upregulated intestinal claudin‐1 expression (p < 0.05). Jejunal (at d 21) and duodenal (at d 42) mucin‐2 expression was up and downregulated by both 50 and 300 mg/kg of cOCM, respectively (p < 0.05). In conclusion, dietary cOCM addition helped to maintain noninflammatory states of humoral and mucosal immunity, and improved the intestinal integrity of broiler chickens.
语种：
英文
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Injury and mechanism of recombinant E. coli expressing STa on piglets colon
作者：
Lv, Yang;Li, Xueni;Zhang, Lin;Shi, Yutao;Du, Linxiao;...
期刊：
Journal of Veterinary Medical Science ,2018年80(2):205-212 ISSN：0916-7250
通讯作者：
Wu, Tao
作者机构：
[Wu, Tao; Gong, Joshua; Lv, Yang; Hou, Yongqing; Li, Xueni; Shi, Yutao; Ding, Binying; Du, Linxiao] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构：
[Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
bacterial toxin;enterotoxin;Escherichia coli protein;glutathione peroxidase;heat stable toxin (E coli);hydrogen peroxide;malonaldehyde;nitric oxide synthase;superoxide dismutase;animal;colon;diarrhea;enterotoxigenic Escherichia coli;Escherichia coli infection;gene expression;genetics;metabolism;microbiology;pathology;pig;real time polymerase chain reaction;veterinary;Animals;Bacterial Toxins;Colon;Diarrhea;Enterotoxigenic Escherichia coli;Enterotoxins;Escherichia coli Infections;Escherichia coli Proteins;Gene Expression;Glutathione Peroxidase;Hydrogen Peroxide;Malondialdehyde;Nitric Oxide Synthase;Real-Time Polymerase Chain Reaction;Superoxide Dismutase;Swine
摘要：
Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 × 109 CFU E. coli LMG194-STa), LMG194 group (2 × 109 CFU E. coli LMG194) and K88 group (2 × 109 CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H2O2; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines. © 2018 The Japanese Society of Veterinary Science.
语种：
英文
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Dietary alpha-ketoglutarate supplementation improves hepatic and intestinal energy status and anti-oxidative capacity of Cherry Valley ducks
作者：
Guo, Shuangshuang;Duan, Rui;Wang, Lei;Hou, Yongqing;Tan, Linglin;...
期刊：
Animal Science Journal ,2017年88(11):1753-1762 ISSN：1344-3941
通讯作者：
Ding, Binying
作者机构：
[Duan, Rui; Cheng, Qiang; Guo, Shuangshuang; Liao, Man; Hou, Yongqing; Tan, Linglin; Wang, Lei; Ding, Binying] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Ding, Binying] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Cherry Valley duck;anti-oxidative capacity;energy status;alpha-ketoglutarate
摘要：
α-Ketoglutarate (AKG) is an extensively used dietary supplement in human and animal nutrition. The aim of the present study was to investigate effects of dietary AKG supplementation on the energy status and anti-oxidative capacity in liver and intestinal mucosa of Cherry Valley ducks. A total of 80 1-day-old ducks were randomly assigned into four groups, in which ducks were fed basal diets supplemented with 0% (control), 0.5%, 1.0% and 1.5% AKG, respectively. Graded doses of AKG supplementation linearly decreased the ratio of adenosine monophosphate (AMP) to adenosine triphosphate (ATP) in the liver, but increased ATP content and adenylate energy charge (AEC) in a quadratic and linear manner, respectively (P < 0.05). Increasing dietary AKG supplemental levels produced linear positive responses in ATP content and AEC, and negative responses in AMP concentration, the ratio of AMP to ATP and total adenine nucleotide in the ileal mucosa (P < 0.05). All levels of dietary AKG reduced the production of jejunal hydrogen peroxide and hepatic malondialdehyde (P < 0.05). Hepatic and ileal messenger RNA expression of AMP kinase α-1 and hypoxia-inducible factor-1α were linearly up-regulated as dietary AKG supplemental levels increased (P < 0.05). In conclusion, dietary AKG supplementation linearly or quadratically enhanced hepatic and intestinal energy storage and anti-oxidative capacity of Cherry Valley ducks.
语种：
英文
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谷氨酰胺对断奶仔猪生长性能、血液生化指标及血浆氨基酸的影响
作者：
李宝成;王蕾;张俊梅;周佳;张越;...
期刊：
黑龙江畜牧兽医 ,2017年(3):123-125 ISSN：1004-7034
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室;武汉轻工大学动物营养与饲料安全湖北省协同创新中心;[朱祖阳] 浙江国茂饲料有限公司;[丁斌鹰; 李抗; 周佳; 李宝成; 侯永清; 张俊梅; 张越; 王蕾; 梅慧敏; 易丹] 武汉轻工大学
关键词：
谷氨酰胺;断奶仔猪;生长性能;血液生化指标;氨基酸
摘要：
为了研究谷氨酰胺对断奶仔猪生长性能、血液生化指标及血浆游离氨基酸含量的影响,试验将16头体重相近的21日龄健康断奶仔猪随机分为2组(对照组和谷氨酰胺组),试验期为21 d,研究在日粮中添加1％谷氨酰胺对仔猪生长性能、血液生化指标及血浆游离氨基酸含量的影响.结果表明:①日粮中添加谷氨酰胺有降低仔猪0～14 d和0～21 d腹泻率的趋势,显著降低了15 ～ 21 d仔猪料重比(P＜0.05).②日粮中添加谷氨酰胺降低了仔猪血液中白细胞数、淋巴细胞绝对值、单核细胞绝对值(P＜0.05).③添加谷氨酰胺可提高血浆尿素、丝氨酸、半胱氨酸、瓜氨酸、蛋氨酸、酪氨酸、β-丙氨酸、γ-氨基丁酸和色氨酸含量(P＜0.05),降低α-氨基丁酸、组氨酸和脯氨酸含量(P＜0.05).说明日粮中添加1％谷氨酰胺可提高仔猪饲料利用率并降低腹泻率,并可改变仔猪血液氨基酸组成,提示谷氨酰胺具有影响部分氨基酸的吸收与转运和改善肠道完整性的作用.
语种：
中文
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N-Acetylcysteine supplementation alleviates intestinal injury in piglets infected by porcine epidemic diarrhea virus
作者：
Wang, Lei;Zhou, Jia;Hou, Yongqing* ;Yi, Dan;Ding, Binying;...
期刊：
Amino Acids ,2017年49(12):1931-1943 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Hu, Chien-An Andy; Zhang, Yue; Zhou, Jia; Hou, Yongqing; Wang, Lei; Xie, Jiaqian; Wu, Guoyao; Ding, Binying; Zhao, Di; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
N-Acetylcysteine;Intestinal functions;Piglets;Porcine epidemic diarrhea virus
摘要：
Porcine epidemic diarrhea virus (PEDV) infects the intestine of young pigs, but effective measures for prevention and treatment are lacking. N-Acetylcysteine (NAC) has been shown to reduce endotoxin-induced intestinal dysfunction. This study was conducted with the PEDV-infected neonatal piglet model to determine the effect of NAC supplementation on intestinal function. Thirty-two 7-day-old piglets were randomly allocated to one of four treatments in a 2×2 factorial design consisting of two liquid diets (0 or 50mg/kg BW NAC supplementation) and oral administration of 0 or 104.5 TCID50 (50% tissue culture infectious dose) PEDV. On day 7 of the trial, half of the pigs (n=8) in each dietary treatment received either sterile saline or PEDV (Yunnan province strain) solution at 104.5 TCID50 per pig. On day 10 of the trial, d-xylose (0.1g/kg BW) was orally administrated to all pigs. One hour later, jugular vein blood samples were collected, and then all pigs were killed to obtain the small intestine. PEDV infection increased diarrhea incidence, while reducing ADG. PEDV infection also decreased plasma d-xylose concentration, small intestinal villus height, mucosal I-FABP and villin mRNA levels but increased mucosal MX1 and GCNT3 mRNA levels (P<0.05). Dietary NAC supplementation ameliorated the PEDV-induced abnormal changes in all the measured variables. Moreover, NAC reduced oxidative stress, as indicated by decreases in plasma and mucosal H2O2 levels. Collectively, these novel results indicate that dietary supplementation with NAC alleviates intestinal mucosal damage and improves the absorptive function of the small intestine in PEDV-infected piglets. © 2017, Springer-Verlag Wien.
语种：
英文
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包被肉桂油对肉鸡生长性能、血浆生化指标、免疫器官指数和肠道形态结构的影响
作者：
段瑞;郭双双;丁斌鹰;李叶涵;程强;...
期刊：
粮食与饲料工业 ,2017年(05):49-54 ISSN：1003-6202
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北 武汉,430023;[毕云天; 丁斌鹰; 段瑞; 程强; 郭双双; 李叶涵] 武汉轻工大学
关键词：
肉桂油;肉鸡;血浆生化指标;免疫器官;肠道形态结构
摘要：
研究旨在探讨日粮中添加不同水平的包被肉桂油对肉鸡生长性能、血浆生化指标、免疫器官指数和肠道形态结构的影响,试验选取1日龄(45.23±0.50)g的科宝(cobb)肉鸡540只,随机分为6个处理组:对照组、正对照组、50mg/kg肉桂油组、100mg/kg肉桂油组、200mg/kg肉桂油组、300mg/kg肉桂油组;每组6个重复,每个重复15只鸡。试验期为42d。结果表明:1)与对照组相比,日粮中添加300mg/kg肉桂油提高了42日龄肉鸡的平均日增重、平均日采食量(P<0.05)。2)日粮中添加不同浓度的肉桂油均降低了肉鸡前期血液中谷丙转氨酶(ALT)活力、谷草转氨酶(AST)活力(P<0.05)。3)日粮中添加50、100 mg/kg的包被肉桂油分别提高了21、42日龄脾脏指数(P<0.05)。4)日粮中添加300mg/kg肉桂油提高了肉鸡前期十二指肠的绒毛高度、空肠绒毛高度/隐窝深度(P<0.05)。综合来看,日粮中添加肉桂油能够提高肉鸡的生长性能,促进脾脏发育,改善血液状况、保持肠道形态结构完整性。包被肉桂油可以替代黄霉素在肉鸡饲料中的应用,适宜添加剂量为300mg/kg。
语种：
中文
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日粮中添加包被肉桂油对肉鸡非特异性免疫功能的影响
作者：
程强;郭双双;丁斌鹰;李叶涵;夏亿
期刊：
中国家禽 ,2017年39(18):28-33 ISSN：1004-6364
作者机构：
武汉轻工大学动物科学与营养工程学院,动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;[丁斌鹰; 程强; 郭双双; 夏亿; 李叶涵] 武汉轻工大学
关键词：
肉鸡;包被肉桂油;非特异性免疫;淋巴细胞增殖
摘要：
试验旨在研究包被肉桂油对肉鸡非特异性免疫功能的影响.选用360只1日龄科宝肉鸡,随机分为6个处理组,每组6个重复,每个重复10只鸡.对照组饲喂玉米-豆粕型基础日粮,正对照组在基础日粮中添加50 mg/kg的黄霉素,试验组分别添加50、100、200和300 mg/kg的包被肉桂油(以肉桂醛计),试验期为42 d.结果显示:与对照组相比,50和200 mg/kg试验组14日龄血液中单核细胞的比例上调(P＜0.05);试验组21日龄血清中溶菌酶的含量增加且呈线性正相关(R2=0.824),试验组42日龄血清中溶茵酶的含量增加且呈线性正相关(R2=0.625);试验组42日龄外周血淋巴细胞的LPS刺激指数上调(P＜0.001).与正对照组相比,50 mg/kg试验组14日龄血液中单核细胞的比例上调(P＜0.05);试验组21日龄血清中溶菌酶的含量增加(P＜0.05);试验组42日龄外周血淋巴细胞的LPS刺激指数上调(P＜0.05).结果表明:日粮中添加包被肉桂油有利于促进肉鸡外周血B淋巴细胞的增殖,增强非特异性免疫功能.
语种：
中文
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N-Acetylcysteine improves intestinal function in lipopolysaccharides-challenged piglets through multiple signaling pathways
作者：
Yi, Dan;Hou, Yongqing* ;Xiao, Hang;Wang, Lei;Zhang, Yue;...
期刊：
Amino Acids ,2017年49(12):1915-1929 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Hu, Chien-An Andy; Zhang, Yue; Xiao, Hang; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
N-Acetylcysteine;Piglets;Intestinal function;Signaling pathway
摘要：
This study determined whether N-acetylcysteine (NAC) could improve intestinal function through phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR), epithelial growth factor receptor (EGFR), toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB), adenosine 5′-monophosphate-activated protein kinase (AMPK), and type I interferon (IFN) signaling pathways in a piglet model of lipopolysaccharides (LPS) challenge. Thirty-two piglets (24-day-old) were randomly allocated to one of four treatments, with eight replicates per treatment and one piglet per replicate. The experiment consisted of four treatments in a 2×2 factorial arrangement with two diets (supplemented with 0 or 500mg NAC/kg diet) and saline or LPS administration. On day 20 of the trial, piglets in the LPS and LPS+NAC groups were intraperitoneally injected with 0 (saline) or 100μg LPS/kg BW. Blood samples were obtained at 3h and intestinal mucosae were collected at 6h post LPS or saline injection. The growth performance was not affected by dietary NAC. LPS induced intestinal dysfunction, as indicated by: (1) reductions in the small-intestinal glutathione concentrations and plasma d-xylose levels; (2) elevations in plasma diamine oxidase activity, mucosal MMP3 mRNA levels and caspase-3 protein abundance; (3) reduced the activities of the small-intestinal mucosal maltase, sucrase and lactase. The adverse effects of LPS on porcine intestinal function and redox status were mitigated by NAC supplementation through the activation of multiple signaling pathways involving PI3K/Akt/mTOR, EGFR, TLR4/NF-κB, AMPK, and type I IFN. Our findings provide novel mechanisms for beneficial effects of NAC in protecting the intestine from inflammation in animals. © 2017, Springer-Verlag Wien.
语种：
英文
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Two Lactobacillus Species Inhibit the Growth and alpha-Toxin Production of Clostridium perfringens and Induced Proinflammatory Factors in Chicken Intestinal Epithelial Cells in Vitro
作者：
Guo, Shuangshuang;Liu, Dan;Zhang, Beibei;Li, Zhui;Li, Yehan;...
期刊：
Frontiers in Microbiology ,2017年8(OCT):2081 ISSN：1664-302X
通讯作者：
Guo, Yuming
作者机构：
[Guo, Shuangshuang; Zhang, Beibei; Li, Zhui; Guo, Yuming; Liu, Dan] China Agr Univ, State Key Lab Anim Nutr, Coll Anim Sci & Technol, Beijing, Peoples R China.;[Guo, Shuangshuang; Li, Yehan; Ding, Binying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.
通讯机构：
[Guo, Yuming] C;China Agr Univ, State Key Lab Anim Nutr, Coll Anim Sci & Technol, Beijing, Peoples R China.
关键词：
Clostridium perfringens;Lactobacillus;chicken;inflammation;intestinal epithelial cell;alpha-toxin
摘要：
Clostridium perfringens is the causative pathogen of avian necrotic enteritis. Lactobacillus spp. are well-characterized probiotics with anti-microbial and immune-modulatory activities. In the present study, we investigated the effects of L. acidophilus and L. fermentum on the growth, α-toxin production and inflammatory responses of C. perfringens. In in vitro culture experiments, both lactobacilli inhibited the growth of C. perfringens (P < 0.01), accompanied with a decrease in pH (P < 0.01). Supernatants from lactobacilli cultures also suppressed the growth of C. perfringens during 24 h of incubation (P < 0.01), but this inhibitory effect disappeared after 48 h. Both lactobacilli decreased the α-toxin production of C. perfringens (P < 0.01) without influencing its biomass, and even degraded the established α-toxin (P < 0.01). Lower environmental pH reduced the α-toxin production as well (P < 0.01). Preincubation with L. acidophilus decreased the attachment of C. perfringens to cells (P < 0.01) with the cell cytotoxicity being unaffected. Both lactobacilli pretreatment reduced the up-regulation of proinflammatory factors, peptidoglycan (PGN) receptors and nuclear factor kappa B (NF-κB) p65 in C. perfringens-challenged chicken intestinal epithelial cells (P < 0.05). In conclusion, L. acidophilus and L. fermentum inhibited the pathological effects of C. perfringens in vitro conditions. © 2017 Guo, Liu, Zhang, Li, Li, Ding and Guo.
语种：
英文
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膨化鸡肝粉的抗氧化能力和体外免疫调节功能
作者：
李叶涵;郭双双;程强;段瑞;毕云天;...
作者机构：
[李叶涵; 郭双双; 程强; 段瑞; 毕云天; 丁斌鹰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
会议论文集名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集
关键词：
膨化鸡肝粉;免疫调节功能;抗氧化能力
摘要：
鸡肝有补肝益肾的功效,鸡肝粉保留了鸡肝的营养成分,具有浓郁的腥香味,适用于各类调味品和宠物食品等产品的原辅料添加,是各种水产动物的优良饲料添加剂和诱食剂。本试验旨在研究膨化鸡肝粉的抗氧化能力和体外免疫功能,以探索其在动物日粮中应用的潜力。将膨化鸡肝粉加入生理盐水中配制成4 mg/mL的悬浊液,充分振荡溶解后,3 000 r/min离心10 min,取上清液。将该上清液倍比稀释至0.4和0.04 m
语种：
中文
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