摘要：
OBJECTIVE: This study aimed to explore the effects of different types of xanthophyll extracted from marigold on the growth performance, skin color, and carcass pigmentation. METHODS: A total of 192 healthy 60-day-old yellow-feathered broilers weighing an average of 1,279±81 g were randomly allocated to 4 groups, each with 6 replicates and 8 broilers. The 4 treatments were as follows: i) CON group, fed with basal diet; ii) LTN group, supplemented with lutein; iii) MDP group, supplemented with monohydroxyl pigment including dehydrated lutein, β-cryptoxanthin, and α-cryptoxanthin; iv) LTN+MDP group, supplemented with lutein and monohydroxyl pigment in proportion to 1:1. The supplementary content of LTN, MDP, and LTN+MDP was 2 g/kg. Skin color was measured after 7, 14, 21, and 28 days of feeding the dietary treatments. The breast, thigh, and abdominal fat of slaughtered chickens were stored in cold storage at 4°C for 24 hours and then the meat color of lightness (L*), redness (a*), and yellowness (b*) values was determined. RESULTS: The results showed that all treatments enhanced the yellow scores of subwing skin on day 14, 21, and 28 (p<0.05), and the mixture of lutein and monohydroxyl pigment promoted the yellow scores of shanks on day 14, 21, and 28 (p<0.05). The mixture of lutein and monohydroxyl pigment increased the yellow scores of beaks and all treatments enhanced the yellow of shanks on day 28 (p<0.05). In addition, all treatments improved the yellow (b*) values of breast and thigh muscle, moreover, the monohydroxyl pigment and the mixture of lutein and monohydroxyl pigment enhanced the values of redness (a*) and yellow (b*) of abdominal fat (p<0.05). CONCLUSION: In summary, different types of xanthophyll extracted from marigold significantly increased the yellow scores of skin color and the yellow (b*) values of carcass pigmentation. Especially, the mixture of lutein and monohydroxyl pigment was more efficient on skin color.
通讯机构：
[Zhang, C ] W;Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Peoples R China.
关键词：
personal audio system;sound field control;acoustic contrast;reconstruction error;array effort
摘要：
A personal audio system has a wide application prospect in people’s lives, which can be implemented by sound field control technology. However, the current sound field control technology is mainly based on sound pressure or its improvement, ignoring another physical property of sound: particle velocity, which is not conducive to the stability of the entire reconstruction system. To address the problem, a sound field method is constructed in this paper, which minimizes the reconstruction error in the bright zone, minimizes the loudspeaker array effort in the reconstruction system, and at the same time controls the particle velocity and sound pressure of the dark zone. Five unevenly placed loudspeakers were used as the initial setup for the computer simulation experiment. Simulation results suggest that the proposed method is better than the PM (pressure matching) and EDPM (eigen decomposition pseudoinverse method) methods in the bright zone in an acoustic contrast index, the ACC (acoustic contrast control) method in a reconstruction error index, and the ACC, PM, and EDPM methods in the bright zone in a loudspeaker array effort index. The average array effort of the proposed method is the smallest, which is about 9.4790, 8.0712, and 4.8176 dB less than that of the ACC method, the PM method in the bright zone, and the EDPM method in the bright zone, respectively, so the proposed method can produce the most stable reconstruction system when the loudspeaker system is not evenly placed. The results of computer experiments demonstrate the performance of the proposed method, and suggest that compared with traditional methods, the proposed method can achieve more balanced results in the three indexes of acoustic contrast, reconstruction error, and loudspeaker array effort on the whole.
作者：
Ye, Yuanyuan;Yan, Wei;Peng, Lijun;He, Jiangling;Zhang, Na;...
期刊：
LWT,2023年175:114470 ISSN：0023-6438
通讯作者：
Jiangling He<&wdkj&>Jie Cai
作者机构：
[He, Jiangling; Cheng, Shuiyuan; Zhou, Jiaojiao; Cai, Jie; Zhang, Na; Ye, Yuanyuan] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Yan, Wei; Peng, Lijun] Hubei Key Lab Nutr Qual & Safety Agroprod, Wuhan 430064, Peoples R China.;[Cai, Jie; Ye, Yuanyuan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Jiangling He; Jie Cai] N;National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China
关键词：
Se-enriched green tea;Mineral;Amino acid;Catechins;Correlation analysis
摘要：
Substance basis of selenium (Se) enriched green tea (Se-GT) plays a crucial role in imparting numerous health benefits to the consumer, however, the complex relationship between minerals, chemical ingredients, and Se in tea leaves remains largely unknown. Here, the contents of 14 minerals and 29 bioactive components (including 19 amino acids, 8 polyphenols, and 2 alkaloids) were determined in five green tea samples with different Se levels. Marked differences were observed in the elemental and chemical profiles of the five groups of teas. Pearson correlation analysis demonstrated that Se was likely to be synergistic with Zn, while antagonistic with Mg, Al, Cr, Fe, and As. Inconspicuous correlations between amino acids and Se in tea leaves were discussed via this extended study. Moreover, Se showed positive correlations with gallic acid, (+)-catechin, and gallated catechins (like (-)-epigallocatechin gallate, (-)-gallocatechin gallate, and (-)-epicatechin gallate), but nega-tively correlated with some non-gallated catechins (including (-)-gallocatechin and (-)-epigallocatechin) in tea leaves. These results suggested that Se has significant effects on the accumulation of minerals and polyphenols in tea leaves. Overall, this study elucidates the elemental and chemical basis of Se-GT and provides a rationale for the quality evaluation of Se-GT, which is of crucial importance for tea industry.
通讯机构：
[Shan Zeng] S;School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, Hubei 430023, China
关键词：
Data augmentation;Anomaly detection;Industrial data
摘要：
Detecting the anomalies in a large amounts of high-dimensional data has been a challenging task. In the Industry 4.0 environment, large-scale high-dimensional monitoring data features the complex pattern of high level semantics. In order to provide enterprise-wide monitoring solutions, it is necessary to identify the high-level semantic patterns of the anomalies in these data without splitting them. Existing end-to-end deep neural networks for time series are capable of recognizing the high-level semantics in natural language or speech signals, but they are barely applied in real-time anomaly detection of industrial data because of the large time costs. In this paper, we leverage the self-supervised contrastive learning methodology and propose a Composite Semantic Augmentation Encoder (CSAE) to provide an appropriate representation of industrial data and implement quick detection of anomalies in industrial application environments. CSAE is a non-sequential deep neural network with two augmentation layers and a mandatory layer. The two layers of data-augmentation are built to expand the size of samples of both low-level semantic anomalies and high-level semantic anomalies, which enables CSAE to discover diverse anomalies and improves its accuracy of high-level semantic pattern recognition. The mandatory layer is built to compress and reserve the temporal information in the industrial data to accelerate the anomaly detection. Therefore, as a non-sequential contrastive learning model, CSAE has faster training convergence than the usual sequence models. The experiment results have verified that CSAE can achieve higher prediction accuracy with less time consumption than existing machine learning models in the tasks of high dimensional anomaly pattern detection. (C) 2022 Elsevier B.V. All rights reserved.
通讯机构：
[Bin Guo] D;Department of Physics, Wuhan University of Technology, Wuhan 430070, China
关键词：
Many -body localization;Two-qubit nonlocality;Multipartite nonlocality;Energy level statistics;Entanglement entropy
摘要：
Characterizing the many-body localization (MBL) transition and revealing its inherent mechanisms from the ergodic phase to the localized phase is an increasing interest issue. In this paper, we use quantum nonlocality, the hierarchy of multipartite correlations, to identify the MBL transition in an XXZ spin chain with random on-site magnetic fields. We use the shift-invert exact diagonalization method to explore the properties of two-qubit nonlocality and multipartite nonlocality in the many-body localized system. We then use their first derivatives to estimate the critical disorder strength, which is found to be range in hc/J is an element of [3, 4]. Correspondingly, two quantities and their first derivatives, the energy level statistics and the half-chain entanglement entropy that are promising for the study of the MBL transition, are also investigated and as comparisons to quantum nonlocality.(c) 2023 Elsevier B.V. All rights reserved.
摘要：
Intestinal health is critical for the growth and development of humans and animals. Our previous study has demonstrated that indomethacin (IDMT) could induce intestinal injury in piglets, and N-acetylcysteine (NAC) supplementation contributed to alleviating intestinal injury induced by various stimuli. In this study, we investigated the mechanism of IDMT-induced cell death in IPEC-1 cell lines and explored the role of NAC by using transcriptomic and proteomic analyses. Results showed that cell viability was substantially reduced with the increasing concentrations of IDMT, whereas NAC significantly increased the survival rate of IPEC-1 cells regardless of its addition method. Transcriptomics and proteomics data indicated that terms, such as cell cycle, energy metabolism, and cell proliferation, were significantly enriched by Gene ontology and pathway analyses. Flow cytometer analysis showed that IDMT induced cell cycle arrest at G0/G1 phase. The expression of cell cycle regulatory proteins (CDK1, CCNA2, and CDC45) was decreased by IDMT stimulation. Importantly, NAC treatment repaired IDMT-induced mitochondrial dysfunction by increasing ATP production, decreasing oxygen consumption rate in non-mitochondrial O 2 consumption, and increasing the red/green fluorescence ratio. IDMT stimulation significantly increased caspase-3 expression, which was partially reversed by NAC treatment. These results suggest that IDMT-induced cell death may be attributable to disturbance of the cell cycle processes, mitochondria dysfunction and apoptosis, and NAC could confer a protective effect by restoring the mitochondrial function and inhibiting the apoptosis pathway. This study provides a theoretical basis for the pathogenesis of IDMT-induced intestinal injury and guides the clinic application of NAC. (c) 2022 Elsevier Inc. All rights reserved.
摘要：
In this study, nanoscale zero valent iron (nZVI) and biochar supported zero valent iron (nZVI/BC) mediated anaerobic digestion of piggery wastewater were investigated. The variation of COD removal rate, methane yield, kinetics modeling and microbial community analysis was analyzed. COD removal rate was increased by 7.46-14.79% respectively for 1, 2, 3 and 4 g nZVI. While COD removal rate was increased by 7.90-12.83% respectively for 1, 2, 3 and 4 g nZVI/BC. Compared with the control group, the accumulated methane yield was increased by 24.17-30.22% and 29.2-46.7% respectively for nZVI and nZVI/BC. Meanwhile, the accumulated hydrogen sulfide concentration was greatly reduced which was decreased from 8.32 ml (control group) to 0.40 ml (nZVI) and 0.22 ml (nZVI/BC). The variation of microbial community showed that nZVI could increase the relative abundance of Methanobacterium, longlinea and syntrophobacter, while nZVI/BC provides a more suitable environment for the growth of Mesotoga, Pertrimonas and Longilinea.
作者机构：
[Guo, S. S.; Li, L. L.; Liu, Z. P.; Ding, B. Y.; Liu, C. A.] Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Zou, X. T.] Zhejiang Univ, Key Lab Mol Anim Nutr, Minist Educ, Hangzhou, Peoples R China.;[Elnesr, S. S.] Fayoum Univ, Dept Poultry Prod, Fac Agr, Al Fayyum 63514, Egypt.
通讯机构：
[Zou, X T] K;Key Laboratory of Molecular Animal Nutrition (Zhejiang University), Ministry of Education, China. Electronic address:
关键词：
calcium signal;salpingitis;uterus;lipopolysaccharide;laying hen
摘要：
This study investigated whether there is disturbance of calcium signal in the simulated salpingitis of laying hens. A total of 90 Roman Pink layers (81 wk; 1.916 ± 0.17 kg) were divided into 3 groups (Control treated with PBS, 1.85 mg lipopolysaccharide (LPS)/layer as LPS group, 1.85 mg LPS/layer as LPS+organic chemical reagent (OCR) group) with 6 replicates of 5 layers. Compared with the Control, the mRNA expression of calcium/calmodulin dependent protein kinase IV (CaMK IV), sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA), and plasma membrane calcium-transporting ATPase (PMCA) were not only decreased (P < 0.05) in magnum of laying hens from LPS and LPS+OCR groups, but also in isthmus and uterus of hens from LPS+OCR group. Moreover, the mRNA expression of calcium sensing receptor (CaSR) and Orai1 in uterus from LPS+OCR group were higher (P < 0.05) than that from Control. The relative fluorescence intensity of Ca(2+) in uterus from LPS and LPS+OCR groups were significantly higher than that from Control (P < 0.05). In conclusion, it existed that the linkage of simulated salpingitis treated with LPS+OCR and altered intracellular calcium signals in layers, which provided a new insight for alleviating salpingitis and uterine dysfunction of laying hens.
摘要：
Tea is one of the most widely consumed nonalcoholic beverages worldwide and one of the most important economic crops in the world. Tea varieties rich in anthocyanins have become one of the important breeding targets due to their important health care function. In our previous study, an important gene CsGSTU18 involved in anthocyanin transport was identified. However, the corresponding molecular mechanism is still unclear. Here, we used the promoter sequence of CsGSTU18 as bait in a yeast onehybrid (YIH) screen for promoter-binding proteins. We identified an R2R3-MYB transcription factor, CsMYBPA1, that was coordinately expressed with anthocyanin accumulation. Molecular evidence confirmed that CsMYBPA1 activated CsGSTU18 by binding to MBS cis-element, indicating that CsGSTU18 was a direct target gene of CsMYBPA1. In addition, over-expression of CsMYBPA1 elevated anthocyanin content in transgenic Arabidopsis. Transient silencing of CsMYBPA1 reduced anthocyanin accumulation and decreased the expression level of CsGSTU18 in tea plant leaves, whereas the transient over-expression of CsMYBPA1 showed the opposite change trend. Overall, these findings suggest that CsMYBPA1 directly actives CsGSTU18 to enhance anthocyanin accumulation. This study provides important gene resources and theoretical basis for the anthocyanin metabolism regulation and modification.
作者机构：
[Wu, Haoqiang; Chen, Yunsi] Wuhan Polytech Univ, Food Econ Res Ctr, Wuhan 430023, Peoples R China.;[Wu, Haoqiang; Chen, Yunsi] Wuhan Polytech Univ, Sch Econ, Wuhan 430048, Peoples R China.;[Hu, Sumin] Suzhou Univ Sci & Technol, Business Sch, Suzhou 215009, Peoples R China.
通讯机构：
[Wu, HQ ] W;Wuhan Polytech Univ, Food Econ Res Ctr, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Econ, Wuhan 430048, Peoples R China.
关键词：
digital economy;green technology innovation;green agricultural development
摘要：
The digital economy is a key driver of greener agriculture and sustainable development. This paper focuses on the impact of the digital economy on green agricultural development and the mediating role of green technology innovation. Using provincial panel data from China from 2011 to 2020, we test hypotheses using fixed effects models. The results indicate that: (a) the digital economy boosts agricultural total factor productivity (AGTFP); (b) green technology innovation positively moderates the relationship between the digital economy and AGTFP; and (c) the positive impact of the digital economy on green agriculture varies across regions, favoring eastern areas.
摘要：
Based on the concept of microstructure control and multiphase doping, a novel strong-textured porous (STP) Cu-Al-Mn (CAM) shape memory alloy was fabricated through multi-stage sintering process. Porosity ranging from 31.1 to 11.4 % and the largest near-elastic deformation approached 8.0% were achieved in the STP Cu71Al18Mn11 alloy. Through in-depth characterization, it is confirmed that STP-CAM has less γ2 phase compared to porous ordinary polycrystalline alloy, and exhibits strong 〈0 0 1〉 and 〈1 0 1〉 -oriented texture along the direction of sintering pressure, as well as low angle grain boundaries, which contributes to the large near-elastic deformation observed in this novel porous SMAs.
关键词：
growth performance;immune function;piglets;yeast hydrolysate;spray-dried plasma protein
摘要：
Yeast hydrolysate (YH) is rich in amino acids, small peptides, B vitamins, glutathione, and nucleotides, which makes it a possible substitute for spray-dried plasma powder (SDPP). This research was conducted to estimate the application of YH instead of SDPP in creep feed of weaned piglets. The experiment had four treatment groups: (1) basal diet (CON group), (2) CON + 4% YH (YH group), (3) CON + 2% YH + 2% SDPP (SY group), and (4) CON + 4% SDPP (SDPP group). Growth performance, biochemical parameters, immunoglobulin levels, and intestinal tissue morphology were measured. No substantial difference in growth performance between the YH, SY, and SDDP groups was found; however, compared with the CON group, the performance of these three groups was substantially improved. The contents of serum globulin and ALP in the CON group were markedly decreased compared to the other groups, but the AST level was substantially increased. The IL-10 concentration in the other groups was substantially higher than the CON group, and the highest content was in group YH; the TNF-alpha content showed an opposite trend. The levels of serum IgG and IgA in the CON group were the lowest among all groups. There were substantial differences among the groups in villi height and crypt depth in the duodenum, jejunum, and ileum. The results showed that YH effectively increased IL-10 concentration and decreased TNF-alpha level to promote intestinal development, while not differing from SDPP in terms of growth performance.
通讯机构：
[Huang Dai] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Food Safety Research Center, Key Research Institute of Humanities and Social Sciences of Hubei Province, Wuhan 430023, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Triterpenoids, known for their anti-inflammatory, anticancer, and hypoglycemic properties, are the major bioactive components in Cyclocarya paliurus (Batal.) Iljinskaja. Selecting elite individuals with high triterpenoids content is the basis of C. paliurus industry for medicinal use. In this study, seasonal variation patterns of total triterpenoids and five triterpene monomers accumulation for three groups with different total triterpenoid contents (TTC; H: 59.74-64.03mgg(-1); M: 47.66-57.08mgg(-1), and L: 35.26-42.22mgg(-1)) were surveyed. Seasonal expression dynamics of 6 key genes relevant to triterpenoids biosynthesis, including HMGR, DXR, SQS, SE, LUS, and β-AS, were described by quantitative real-time PCR (qRT-PCR) for three groups. The expression levels of HMGR, SE, LUS, and β-AS genes in group H were higher than in groups M and L. In addition, Pearson correlation analysis showed that they were significantly positively correlated with triterpene accumulation, and the expression level of SE gene not only was significantly correlated with downstream genes, but also exhibited a linear relationship with TTC, especially in September. These results suggest that SE gene could serve as an effective make for screening elite individuals with high TTC from the germplasm of C. paliurus for medicinal use. Further testing on randomly selected individuals in next September proved the feasibility and reliability of SE gene in assisted selection. Also, we successfully cloned the full-length cDNA of SE. Thus, our work provides an efficient way to attain superior genotypes to develop medicinal industry of C. paliurus in practice.
摘要：
Accurate classification and identification of chicken parts are critical to improve the productivity and processing speed in poultry processing plants. However, the overlapping of chicken parts has an impact on the effectiveness of the identification process. To solve this issue, this study proposed a real-time classification and detection method for chicken parts, utilizing YOLOV4 deep learning. The method can identify segmented chicken parts on the assembly line in real time and accurately, thus improving the efficiency of poultry processing. First, 600 images containing multiple chicken part samples were collected to build a chicken part dataset after using the image broadening technique, and then the dataset was divided according to the 6:2:2 division principle, with 1200 images as the training set, 400 images as the test set, and 400 images as the validation set. Second, we utilized the single-stage target detector YOLO to predict and calculate the chicken part images, obtaining the categories and positions of the chicken leg, chicken wing, and chicken breast in the image. This allowed us to achieve real-time classification and detection of chicken parts. This approach enabled real-time and efficient classification and detection of chicken parts. Finally, the mean average precision (mAP) and the processing time per image were utilized as key metrics to evaluate the effectiveness of the model. In addition, four other target detection algorithms were introduced for comparison with YOLOV4-CSPDarknet53 in this study, which include YOLOV3-Darknet53, YOLOV3-MobileNetv3, SSD-MobileNetv3, and SSD-VGG16. A comprehensive comparison test was conducted to assess the classification and detection performance of these models for chicken parts. Finally, for the chicken part dataset, the mAP of the YOLOV4-CSPDarknet53 model was 98.86% on a single image with an inference speed of 22.2 ms, which was higher than the other four models of YOLOV3-Darknet53, YOLOV3-MobileNetv3, SSD-MobileNetv3, and SSD-VGG16 mAP by 3.27%, 3.78%, 6.91%, and 6.13%, respectively. The average detection time was reduced by 13, 1.9, 6.2, and 20.3 ms, respectively. In summary, the chicken part classification and detection method proposed in this study offers numerous benefits, including the ability to detect multiple chicken parts simultaneously, as well as delivering high levels of accuracy and speed. Furthermore, this approach effectively addresses the issue of accurately identifying individual chicken parts in the presence of occlusion, thereby reducing waste on the assembly line. PRACTICAL APPLICATION: The aim of this study is to offer visual technical assistance in minimizing wastage and resource depletion during the sorting and cutting of chicken parts in poultry production and processing facilities. Furthermore, considering the diverse demands and preferences regarding chicken parts, this research can facilitate product processing that caters specifically to consumer preferences.
作者机构：
[He, Jie; He, J; Li, Xiaoyan] Chengdu Med Coll, Clin Med Coll, Chengdu, Peoples R China.;[He, Jie; He, J] Chengdu Med Coll, Dept Resp & Crit Care Med, Affiliated Hosp 1, Chengdu, Peoples R China.;[Li, Xiaoyan] Chengdu Med Coll, Dept Endocrinol, Affiliated Hosp 1, Chengdu, Peoples R China.
通讯机构：
[He, J ] C;Chengdu Med Coll, Clin Med Coll, Chengdu, Peoples R China.;Chengdu Med Coll, Dept Resp & Crit Care Med, Affiliated Hosp 1, Chengdu, Peoples R China.
关键词：
aging;IPF;bioinformatics analysis;gene expression omnibus dataset;gene
摘要：
Aging plays a significant role in the occurrence and development of idiopathic pulmonary fibrosis (IPF). In this study, we aimed to identify and verify potential aging-associated genes involved in IPF using bioinformatic analysis. The mRNA expression profile dataset GSE150910 available in the Gene Expression Omnibus (GEO) database and R software were used to identify the differentially expressed aging-related genes involved in IPF. Hub gene expression was validated by other GEO datasets. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on differentially expressed aging-related genes. Subsequently, aging-related genes were further screened using three techniques (least absolute shrinkage and selection operator (LASSO) regression, support vector machine, and random forest), and the receiver operating characteristic curves were plotted based on screening results. Finally, real-time quantitative polymerase chain reaction (qRT-PCR) was performed to verify the RNA expression of the six differentially expressed aging-related genes using the blood samples of patients with IPF and healthy individuals. Sixteen differentially expressed aging-related genes were detected, of which the expression of 12 were upregulated and four were downregulated. GO and KEGG enrichment analyses indicated the presence of several enriched terms related to senescence and apoptotic mitochondrial changes. Further screening by LASSO regression, support vector machine, and random forest identified six genes (IGF1, RET, IGFBP2, CDKN2A, JUN, and TFAP2A) that could serve as potential diagnostic biomarkers for IPF. Furthermore, qRT-PCR analysis indicated that among the above-mentioned six aging-related genes, only the expression levels of IGF1, RET, and IGFBP2 in patients with IPF and healthy individuals were consistent with the results of bioinformatic analysis. In conclusion, bioinformatics analysis identified 16 potential aging-related genes associated with IPF, and clinical sample validation suggested that among these, IGF1, RET, and IGFBP2 might play a role in the incidence and prognosis of IPF. Our findings may help understand the pathogenesis of IPF.
关键词：
Ghrelin;angiogenic factor with G-patch and FHA domain 1;subarachnoid hemorrhage;early cerebral injuries;oxidative stress
摘要：
The objective of the research was to investigate action mechanism of oxidative stress and cerebral injuries after subarachnoid hemorrhage (SAH) by Ghrelin and angiogenic factor G-patch and FHA domain 1 (Aggf1) and offer new research ideas to SAH clinical treatment and SAH-induced early cerebral injuries. SAH rat models were prepared by prechiasmatic anterior cistern injection. Specific Ghrelin and Aggf1 small interfering ribonucleic acid (siRNA) were designed and injected into silence Ghrelin or Aggf1 in rat left lateral ventricles. Rats were divided randomly into sham-operated (sham), SAH model, negative control siRNA, Ghrelin silence (Ghrelin((-/-))), and Aggf1 silence groups. Changes of rat neurological impairment, encephaledema, cerebral tissue phosphorylated protein kinase (p-Akt), and content changes of caspase-3 protein and oxidative stress indexes were observed, including glutathione (GSH) and oxidized glutathione (GSSG). Results showed scores of neurological impairment and water content in SAH model group were reduced compared with sham group, while p-Akt protein and GSH contents were enhanced. However, caspase-3 protein and GSSG contents were declined, showing statistically meaningful difference (P < 0.05). Compared with SAH model group, scores of neurological impairment, cerebral tissue water content, and caspase-3 protein and GSSG contents in silence Ghrelin and Aggf1 groups were increased, while p-Akt protein and GSH contents were decreased, demonstrating statistically meaningful difference (P < 0.05). To conclude, silence Ghrelin and Aggf1 aggravated early cerebral injuries after SAH, revealing that Ghrelin and Aggf1 could protect brains to some degree.
关键词：
Biomass;Carbonized bacterial cellulose;Cr(VI) adsorption;Pyrolysis temperature
摘要：
The effects of pyrolysis temperature on properties and adsorption performance of carbonized bacterial cellulose (CBC) produced from bacterial cellulose at 300, 400, 600 and 800 degrees C were investigated. As pyrolysis temperature increased, the BET surface area, C and ash contents of CBC increased while its mass yield and the contents of H, N and O decreased. Higher pyrolysis temperature resulted in CBC having more aromatic structure and less hydrophilic. The impacts of pyrolysis temperature, solution pH, contact time and initial concentration on the absorption of Cr(VI) onto CBC were systematically studied as well. The results showed that CBC400 prepared at 400 degrees C exhibited the highest Cr(VI) adsorption capacity for Cr(VI) up to 250.0 mg/g. The equilibrium adsorption and adsorption kinetics fitted the Langmuir isotherm and pseudo-second-order kinetic models well. The mechanisms of adsorption of Cr(VI) on CBC included electrostatic interaction, pi-pi interaction and functional groups complexation.
