作者： Li, Han;Wan, Yanjian;Chen, Xiao;Cheng, Lu;Yang, Xueyu;...

期刊： Chemosphere,2018年199(May):702-708 ISSN：0045-6535

通讯作者： Xu, Shunqing;Zhang, Hongling

作者机构： [Xia, Wei; Li, Han; Xu, Shunqing; Cheng, Lu; Yang, Xueyu] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Key Lab Environm & Hlth,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;[Xia, Wei; Li, Han; Xu, Shunqing; Cheng, Lu; Yang, Xueyu] Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Minist Environm Protect,State Key Lab Environm Hl, Wuhan 430030, Hubei, Peoples R China.;[Wan, Yanjian] Wuhan Ctr Dis Control & Prevent, Inst Environm Hlth, Wuhan 430015, Hubei, Peoples R China.;[Chen, Xiao] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Hongling] Wuhan Polytech Univ, Sch Hlth Sci & Nursing, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Xu, Shunqing] H;[Zhang, Hongling] W;Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Key Lab Environm & Hlth,Minist Educ, Wuhan 430030, Hubei, Peoples R China.;Huazhong Univ Sci & Technol, Sch Publ Hlth, Tongji Med Coll, Minist Environm Protect,State Key Lab Environm Hl, Wuhan 430030, Hubei, Peoples R China.;Wuhan Polytech Univ, Sch Hlth Sci & Nursing, Wuhan 430023, Hubei, Peoples R China.

关键词： Nickel;Particulate matter;Human exposure;Outdoor air

摘要： Nickel is a widespread environmental contaminant, and it is toxic to humans in certain forms at high doses. Despite this, nationwide data on nickel in outdoor air particulate matter and human exposure to nickel through inhalation in China are limited. In the present study, 662 outdoor air samples from seven representative provinces such as Shanghai, Hubei, Hunan, Hebei, Guangdong, Yunnan, and Shanxi were collected between March 2013 and February 2014 and analyzed by inductively coupled plasma mass spectrometry. The concentrations of nickel in the air were in the range of 2.1–80.9 ng/m3 (geometric mean: 14.4 ng/m3). In most areas, the concentrations of nickel were higher in winter and spring than those measured in summer and autumn. The daily intake (median) of nickel through inhalation of air particulate matter was estimated. Although the nickel concentrations in some air samples were high, inhalation of the air particulate matter accounted for a minor part of the total nickel intake; however, the adverse effects of human exposure to nickel through inhalation and its potential sources require more attention, particularly in Shanghai. This is a multiregional survey of nickel in outdoor air particulate matter in China. © 2018 Elsevier Ltd

语种： 英文

作者： Li, Yan;Wang, Pengcheng;Hu, Chuanlin;Wang, Kun;Chang, Qing;...

期刊： Scientific Reports,2018年8(1):1939 ISSN：2045-2322

通讯作者： Gong, Zhiyong;Wu, Yang

作者机构： [Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Hubei, Peoples R China.;[Wu, Yang; Gong, Zhiyong; Wu, Y] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Wang, Kun] NYU, Dept Populat Hlth, Div Biostat, 550 1st Ave, New York, NY 10016 USA.;[Chang, Qing] Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Kowloon, Hong Kong, Peoples R China.

通讯机构： [Gong, ZY; Wu, Y] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.

摘要： Exposure to PM2.5 has become one of the most important factors affecting public health in the world. Both clinical and research studies have suggested that PM2.5 inhalation is associated with impaired lung function. In this study, material characterization identified the existence of nanoscale particulate matter (NPM) in airborne PM2.5 samples. When coming into contact with protein-rich fluids, the NPM becomes covered by a protein layer that forms a "protein corona". Based on a 3D organotypic cell culture, the protein corona was shown to mitigate NPM cytotoxicity and further stimulate the proliferation of human lung fibroblasts (HLFs). ROS-activated alpha-smooth muscle actin (alpha-SMA) is considered to be one of the proliferation pathways. In this research, 3D cell cultures exhibited more tissue-like properties compared with the growth in 2D models. Animal models have been widely used in toxicological research. However, species differences make it impossible to directly translate discoveries from animals to humans. In this research, the 3D HLF model could partly simulate the biological responses of NPM-protein corona-induced aberrant HLF proliferation in the human lung. Our 3D cellular results provide auxiliary support for an animal model in research on PM2.5-induced impaired lung function, particularly in lung fibrosis.

语种： 英文

作者： Zhang, Xi-Feng;Huang, Feng-Hua;Zhang, Guo-Liang;Bai, Ding-Ping;Massimo, De Felici;...

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2017年12:7551-7575 ISSN：1178-2013

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Huang, Feng-Hua; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Zhang, Xi-Feng] Qingdao Agr Univ, Inst Reprod Sci, Qingdao, Peoples R China.;[Zhang, Guo-Liang] Dong EE Jiao Co Ltd, Natl Engn Res Ctr Gelatin Based Tradit Chinese Me, Donge, Shandong, Peoples R China.;[Bai, Ding-Ping; Huang, Yi-Fan] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou, Fujian, Peoples R China.;[Massimo, De Felici] Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, 120 Gwangjin Gu, Seoul 143701, South Korea.

关键词： graphene;trichostatin;cytotoxicity;reactive oxygen species;apoptosis;DNA fragmentation;double-strand DNA breaks

摘要： Background: Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). Methods: The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. Results: AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis. Conclusion: This study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression. © 2017 Zhang et al.

语种： 英文

作者： Geng, Peiling;Hu, Yimin;Zhou, Guoping;Yuan, Zhiming*;Hu, Xiaomin*

期刊： International Journal of Food Microbiology,2017年241:291-297 ISSN：0168-1605

通讯作者： Yuan, Zhiming;Hu, Xiaomin

作者机构： [Geng, Peiling; Yuan, Zhiming; Hu, Xiaomin; Hu, Yimin] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430070, Peoples R China.;[Geng, Peiling] Univ Chinese Acad Sci, Beijing 100039, Peoples R China.;[Zhou, Guoping] Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Yuan, ZM; Hu, Xiaomin] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China.

通讯机构： [Yuan, ZM; Hu, XM] C;Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China.

关键词： Bacillus cereus;Bactericidal enzymes;Cocktail;Emesis;Foodstuff

摘要： Bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases. It is therefore necessary to develop an effective strategy to prevent the propagation of B. cereus in the food supply. In this study, three autolysins from the cereulide-producing B. cereus group isolates, LysIS075, LysF8819.1 and Ly5CER057, were identified and characterized. The results showed that the three autolysins were highly lytic and bactericidal to the tested cereulide-producing B. cereus group strains and cross-lytic against other tested B. cereus group strains, and they could inhibit the spore germination and propagation of their tested derived emetic strains. Physical and chemical characterization showed that all the three autolysins were alkalophilic with the optimal activity at pH 9.0 or 9.5 with one exception of LysF8819.1 also having significant lytic activity at pH 5.0, and they all had relative strong lyric activity at 37-50 degrees C during the 30 minute assay. However, LysCER057 showed relative susceptibility to thermo-condition. Remarkably, the separate or cock-tail addition of the three autolysins in food matrices (milk and rice porridge) showed effective bactericidal activity within the tested 2 h. All the results revealed that the three autolysins might be potential candidates to control emetic B. cereus strains in different applications. (C) 2016 Published by Elsevier B.V.

语种： 英文

作者： Liu, Xiaoyun;Wei, Wei*;Zhu, Wenjun;Su, Lufang;Xiong, Zeyang;...

期刊： 分子植物,2017年10(12):1510-1522 ISSN：1674-2052

通讯作者： Wei, Wei;Zhou, Dao-Xiu

作者机构： [Su, Lufang; Zheng, Yu; Liu, Xiaoyun; Wei, Wei; Xiong, Zeyang; Zhou, Man] Jianghan Univ, Inst Interdisciplinary Res, Wuhan 430056, Hubei, Peoples R China.;[Zhou, Dao-Xiu] Univ Paris Saclay, Univ Paris Sud 11, INRA, Inst Plant Sci Paris Saclay IPS2,CNRS, B630, F-91405 Orsay, France.;[Zhu, Wenjun] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Wei, Wei] J;[Zhou, Dao-Xiu] U;Jianghan Univ, Inst Interdisciplinary Res, Wuhan 430056, Hubei, Peoples R China.;Univ Paris Saclay, Univ Paris Sud 11, INRA, Inst Plant Sci Paris Saclay IPS2,CNRS, B630, F-91405 Orsay, France.

关键词： chromartin;histone modification;HDAC;sirtuin;lysine acetylation;metabolism

摘要： How plant metabolic flux alters gene expression to optimize plant growth and response to stress remains largely unclear. Here, we report that Arabidopsis thaliana NAD~+-dependent histone deacetylase AtSRT1 negatively regulates plant tolerance to stress and glycolysis but stimulates mitochondrial respiration. We found that AtSRT1 interacts with Arabidopsis cMyc-Binding Protein 1 (AtMBP-1), a transcriptional repressor produced by alternative translation of the cytosolic glycolytic enolase gene LOS2/ENO2. We demonstrated that AtSRT1 could associate with the chromatin of AtMBP-1 targets LOS2/ENO2 and STZ/ZAT10, both of which encode key stress regulators, and reduce the H3K9ac levels at these genes to repress their transcription. Overexpression of both AtSRT1 and AtMBP-1 had synergistic effects on the expression of glycolytic genes, glycolytic enzymatic activities, and mitochondrial respiration. Furthermore, we found that AtMBP-1 is lysine-acetylated and vulnerable to proteasomal protein degradation,while AtSRT1 could remove its lysine acetylation and significantly enhance its stability in vivo. Taken together, these results indicate that AtSRT1 regulates primary metabolism and stress response by both epigenetic regulation and modulation of AtMBP-1 transcriptional activity in Arabidopsis.

语种： 英文

作者： Chen, Yu-Lian;Xiao, Chuan-Hao;Hu, Zhi-Xiong;Liu, Xiao-Shan;Liu, Zhiguo;...

期刊： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017年1055-1056:165-171 ISSN：1570-0232

通讯作者： Zhao, Xiu-Ju（赵秀举）;Zhang, Wei-Nong

作者机构： [Zhao, Xiu-Ju; Hu, Zhi-Xiong; Liu, Zhiguo; Liu, Xiao-Shan; Chen, Yu-Lian] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Zhang, Wei-Nong] Wuhan Polytech Univ, Sch Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Xiao, Chuan-Hao] Puyang Vocat & Tech Coll, Puyang 457000, Peoples R China.

通讯机构： [Zhao, Xiu-Ju; Zhang, Wei-Nong] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.

关键词： Biomarkers of serum fatty acids;GC-MS;Hyperlipidemia

摘要： Biomarkers of serum fatty acids in hyperlipidemia need to be elucidated. 90 SPF KM male mice were randomly divided into 18 groups (n = 5/group), control groups, and high fat diet (HFD) groups at 9 time points. On day 7, 10, 15, 18, 21, 24, 28, 31, and 35, the mice were sacrificed;blood was collected into tubes from the eyes, serum samples for clinical biochemistry assays and gas chromatography–mass spectroscopy were attained after centrifugation, and the contents of serum fatty acids were detected with GC–MS. Sections of livers were taken and stored in formalin solution for histological assessments. No species differences existed in all these groups. The contents of C16:1, C18:1, C22:6 were significantly different between HFD groups and the corresponding controls;meanwhile, the proportion of fatty acids, especially the monounsaturated degree, the polyunsaturated degree, changed significantly and regularly (P < 0.05). Thus the three unsaturated fatty acids C16:1, C18:1, C22:6 and the monounsaturated/polyunsaturated unsaturated degrees may be as potential biomarkers of hyperlipidemia. ©2017 Elsevier B.V.

语种： 英文

作者： Zhu, Wenjun;Ronen, Mordechi;Gur, Yonatan;Minz-Dub, Anna;Masrati, Gal;...

期刊： PLANT PHYSIOLOGY,2017年175(1):438-456 ISSN：0032-0889

通讯作者： Sharon, Amir

作者机构： [Zhu, Wenjun; Ronen, Mordechi; Eizner, Elad; Sharon, Itai; Gur, Yonatan; Sharon, Amir; Minz-Dub, Anna] Tel Aviv Univ, Fac Life Sci, Dept Mol Biol & Ecol Plants, IL-69978 Tel Aviv, Israel.;[Zhu, Wenjun] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Masrati, Gal; Ben-Tal, Nir] Tel Aviv Univ, Fac Life Sci, Dept Biochem & Mol Biol, IL-69978 Tel Aviv, Israel.;[Savidor, Alon] Weizmann Inst Sci, Nancy & Stephen Grand Israel Natl Ctr Personalize, IL-76100 Rehovot, Israel.;[Eizner, Elad] Tel Aviv Univ, Fleischman Fac Engn, Dept Phys Elect, IL-69978 Tel Aviv, Israel.

通讯机构： [Sharon, Amir] T;Tel Aviv Univ, Fac Life Sci, Dept Mol Biol & Ecol Plants, IL-69978 Tel Aviv, Israel.

摘要： In search of Botrytis cinerea cell death-inducing proteins, we found a xyloglucanase (BcXYG1) that induced strong necrosis and a resistance response in dicot plants. Expression of the BcXYG1 gene was strongly induced during the first 12 h post inoculation, and analysis of disease dynamics using PathTrack showed that a B. cinerea strain overexpressing BcXYG1 produced early local necrosis, supporting a role of BcXYG1 as an early cell death-inducing factor. The xyloglucanase activity of BcXYG1 was not necessary for the induction of necrosis and plant resistance, as a mutant of BcXYG1 lacking the xyloglucanase enzymatic activity retained both functions. Residues in two exposed loops on the surface of BcXYG1 were found to be necessary for the induction of cell death but not to induce plant resistance. Further analyses showed that BcXYG1 is apoplastic and possibly interacts with the proteins of the plant cell membrane and also that the BcXYG1 cell death-promoting signal is mediated by the leucine-rich repeat receptor-like kinases BAK1 and SOBIR1. Our findings support the role of cell death-inducing proteins in establishing the infection of necrotrophic pathogens and highlight the recognition of fungal apoplastic proteins by the plant immune system as an important mechanism of resistance against this class of pathogens.

语种： 英文

作者： Liu, Jing-Cai;Lai, Fang-Nong;Li, Ling;Sun, Xiao-Feng;Cheng, Shun-Feng;...

期刊： CELL DEATH & DISEASE,2017年8(8):e2966 ISSN：2041-4889

通讯作者： Shen, Wei

作者机构： [Liu, Jing-Cai; Lai, Fang-Nong; Ge, Wei; Sun, Xiao-Feng; Shen, Wei; Li, Lan; Cheng, Shun-Feng; Wang, Yu-Feng] Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.;[Li, Ling] Tengzhou Peoples Hosp, Tengzhou 277500, Peoples R China.;[Sun, Xiao-Feng] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[De Felici, Massimo] Univ Roma Tor Vergata, Dept Biomed & Prevent, I-00133 Rome, Italy.

通讯机构： [Shen, Wei] Q;Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.

会议名称： 中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”

会议时间： 2017-08-25

会议地点： 中国安徽合肥

会议论文集名称： 中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”论文集

关键词： DEHP;Mice;Oogenesis;Meiotic entry;DNA damage

摘要： Di (2-ethylhexyl) phthalate (DEHP), is the most common member of the class of phthalates that are used as plasticizers and have become common environmental contaminants. A number of studies have shown that DEHP exposure impacts reproductive health in both male and female mammals by acting as an estrogen analog. Here, we investigated the effects of DEHP on meiotic progression of fetal mouse oocytes by using an in vitro model of ovarian tissue culture. The results showed that 10 or 100 ?M DEHP exposure inhibited the progression of oocytes throughout meiotic prophase I, specifically from the pachytene to diplotene stages. DEHP possibly impairs the ability to repair DNA double-strand breaks induced by meiotic recombination and as a consequence activates a pachytene check point. At later stages, such defects led to an increased number of oocytes showing apoptotic markers (TUNEL staining, expression of pro-Apoptotic genes), resulting in reduced oocyte survival, gap junctions, and follicle assembly in the ovarian tissues. Microarray analysis of ovarian tissues exposed to DEHP showed altered expression of several genes including some involved in apoptosis and gonad development. The expression changes of some genes clustered in cell-cell communication and signal transduction, along with plasma membrane, extracellular matrix and ion channel function classes, were dependent on the DEHP concentration. Together, these results bring new support to the notion that exposure to DEHP during gestation might exert deleterious effects on ovary development, perturbing germ cell meiosis and the expression of genes involved in a wide range of biological processes including ovary development.

语种： 英文

作者： Lai, Fang-Nong;Liu, Jing-Cai;Li, Lan;Ma, Jun-Yu;Liu, Xue-Lian;...

期刊： Archives of Toxicology,2017年91(3):1279-1292 ISSN：0340-5761

通讯作者： Shen, Wei

作者机构： [Liu, Jing-Cai; Lai, Fang-Nong] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China.;[Liu, Jing-Cai; Lai, Fang-Nong; Ma, Jun-Yu; Liu, Xue-Lian; Shen, Wei; Li, Lan; Zhang, Xi-Feng] Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Chen, Hong; Li, Lan] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China.;[Liu, Yu-Ping] Qingdao Agr Univ, Hosp Sch, Qingdao 266109, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

通讯机构： [Shen, Wei] Q;Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.

关键词： Di (2-ethylhexyl) phthalate;Pre-puberty mice;Folliculogenesis;Steroidogenesis

摘要： Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer which is widely used in the manufacture of plastics. As a common environmental contaminant and recognized endocrine disrupting chemical, DEHP is able to deregulate the functions of a variety of tissues, including the reproductive system both in males and females. In order to investigate the possible effects of DEHP on the first wave of folliculogenesis, occurring in the mouse ovary postnatally, mice were administered 20 or 40 μg/kg DEHP through intraperitoneal injection at days 5, 10 and 15 post partum (dpp). Following DEHP treatment the gene expression profile of control and exposed ovaries was compared by microarray analyses at 20 dpp. We found that in the exposed ovaries DEHP significantly altered the transcript levels of several immune response and steroidogenesis associated genes. In particular, DEHP significantly decreased the expression of genes essential for androgen synthesis by theca cells including Lhcgr, Cyp17a1, Star and Ldlr. Immunohistochemistry and immune flow cytometry confirmed reduced expression of LHCGR and CYP17A1 proteins in the exposed theca cells. These effects were associated to a significant reduction in ovarian concentrations of progesterone, 17β-estradiol and androstenedione along with a reduction of LH in the serum. Although we did not find a significant reduction of the number of primary, secondary or antral follicles in the DEHP exposed ovaries when compared to controls, we did observe that theca cells showed an altered structure of the nuclear envelope, fewer mitochondria, and mitochondria with a reduced number of cristae. Collectively, these results demonstrate a deleterious effect of DEHP exposure on ovarian steroidogenesis during the first wave of folliculogenesis that could potentially affect the correct establishment of the hypothalamic-pituitary-ovarian axis and the onset of puberty. © 2016, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： Yang, Jiang-Ke*;Xiong, Wei;Chen, Fang-Yuan;Xu, Li;Han, Zheng-Gang

期刊： PLOS ONE,2017年12(5):e0176444- ISSN：1932-6203

通讯作者： Yang, Jiang-Ke

作者机构： [Xu, Li; Han, Zheng-Gang; Chen, Fang-Yuan; Yang, Jiang-Ke; Xiong, Wei] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Peoples R China.

通讯机构： [Yang, Jiang-Ke] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Peoples R China.

关键词： Cellulose;Aromatic amino acids;Cell binding;Cell binding assay;Cellulases;Facies (medical);Filter paper;Binding analysis

摘要： The cellulose binding domain (CBD) of cellulase binding to cellulosic materials is the initiation of a synergistic action on the enzymatic hydrolysis of the most abundant renewable biomass resources in nature. The binding of the CBD domain to cellulosic substrates generally relies on the interaction between the aromatic amino acids structurally located on the flat face of the CBD domain and the glucose rings of cellulose. In this study, we found the CBD domain of a newly cloned Penicillium crustosum endoglucanase EGL1, which was phylogenetically related to Aspergillus, Fusarium and Rhizopus, and divergent from the well-characterized Trichoderma reeseis cellulase CBD domain, contain two conserved aromatic amino acid-rich regions, Y451-Y452 and Y477-Y478-Y479, among which three amino acids Y451, Y477, and Y478 structurally sited on a flat face of this domain. Cellulose binding assays with green fluorescence protein as the marker, adsorption isotherm assays and an isothermal titration calorimetry assays revealed that although these three amino acids participated in this process, the Y451-Y452 appears to contribute more to the cellulose binding than Y477-Y478-Y479. Further glycine scanning mutagenesis and structural modelling revealed that the binding between CBD domain and cellulosic materials might be multi-amino-acids that participated in this process. The flexible poly-glucose molecule could contact Y451, Y477, and Y478 which form the contacting flat face of CBD domain as the typical model, some other amino acids in or outside the flat face might also participate in the interaction. Thus, it is possible that the conserved Y451-Y452 of CBD might have a higher chance of contacting the cellulosic substrates, contributing more to the affinity of CBD than the other amino acids.

语种： 英文

作者： Wang, Hualin;Sit, Wat-Hung;Tipoe, George Lim;Liu, Zhiguo;Wan, Jennifer Man-Fan*

期刊： Clinical Nutrition,2017年36(1):198-208 ISSN：0261-5614

通讯作者： Wan, Jennifer Man-Fan

作者机构： [Wang, Hualin; Liu, Zhiguo] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Wang, Hualin; Wan, Jennifer Man-Fan; Sit, Wat-Hung] Univ Hong Kong, Sch Biol Sci, Food & Nutr Div, Hong Kong, Hong Kong, Peoples R China.;[Tipoe, George Lim] Univ Hong Kong, Li Ka Shing Fac Med, Dept Anat, Hong Kong, Hong Kong, Peoples R China.

通讯机构： [Wan, Jennifer Man-Fan] U;Univ Hong Kong, Sch Biol Sci, Food & Nutr Div, Hong Kong, Hong Kong, Peoples R China.

关键词： Carbon tetrachloride;Corn oil;Lard;Liver fibrosis;Proteomics

摘要： Introduction The influences of dietary fatty acids on the progress of chronic liver diseases have attracted lots of attentions, but the mechanisms of the effects of lipids rich in saturated fatty acids or PUFAs on hepatic fibrogenesis remain unclear. Methods Female Fischer 344 rats were fed normal chow or chow plus 20% (w/w) of corn oil or lard, respectively, and injected CCl4 twice a week for 4 weeks to induce liver fibrosis. Masson's staining was adopted to illustrate the fibrosis level. The mRNA expression level of α-SMA and the DNA methylation level of its promoter region were analyzed. A 2-DE gel based proteomic approach was constructed to investigate the differential expression level of hepatic proteome between three diet groups. Results Histological evaluations and α-SMA expression analysis illustrated the high corn oil intake has no effects on hepatic fibrogenesis, but lard intake aggravated liver fibrosis, partly attributed to DNA demethylation of α-SMA promoter region. 2-DE Gel based proteomic study demonstrated excessive lard consumption elevated the expression of fibrosis related alpha-1-antitrypsin precursor, and endoplasmic reticulum stress related proteins such as heat shock cognate 71 kDa, eukaryotic translation initiation factor 4A1 and protein disulfide isomerase associated 3. Moreover, unlike corn oil rich in PUFAs, lard had no effects to elevate the expression of glutathione S-transferases, but decreased the expression of iron store related proteins heme binding protein 1 and ferritin. Conclusions Lard intake aggravates CCl4 induced liver fibrosis via enhancing the expression of fibrogenesis and ER stress related proteins, and disturbing the hepatic transmethylation reaction. © 2015 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism

语种： 英文

作者： He, Lei*;Yuan, Fa-hu;Chen, Ting;Huang, Qiang;Wang, Yu;...

期刊： 当代医学科学,2017年37(2):217-225 ISSN：2096-5230

通讯作者： He, Lei;Liu, Zhi-guo

作者机构： [He, Lei] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Blood Transfus, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Yuan, Fa-hu] Jianghan Univ, Sch Med, Wuhan 430000, Peoples R China.;[Huang, Qiang; Chen, Ting; Liu, Zhi-guo] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Wang, Yu] Huazhong Univ Sci & Technol, Sch Basic Med, Dept Biochem & Mol Biol, Tongji Med Coll, Wuhan 430030, Peoples R China.

通讯机构： [He, Lei] H;[Liu, Zhi-guo] W;Huazhong Univ Sci & Technol, Tongji Hosp, Dept Blood Transfus, Tongji Med Coll, Wuhan 430030, Peoples R China.;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

关键词： fibronectin;ER stress;nonalcoholic fatty liver disease;necrosis

摘要： Fibronectin containing extra domain A (EDA+ FN), a functional glycoprotein participating in several cellular processes, correlates with chronic liver disease. Herein, we aim to investigate the expression and secretion of EDA+ FN from hepatocytes in nonalcoholic fatty liver disease (NAFLD) and the underlying mechanisms. Circulating levels of EDA+ FN were determined by ELISA in clinical samples. Western blotting and flow cytometry were performed on L02 and HepG2 cell lines to analyze whether the levels of EDA+ FN were associated with endoplasmic reticulum (ER) stress-related cell death. Circulating levels of EDA+ FN in NAFLD patients were significantly higher than those in control subjects, and positively related with severity of ultrasonographic steatosis score. In cultured hepatocytes, palmitate up-regulated the expression of EDA+ FN in a dose-dependent manner. Conversely, when the cells were pretreated with 4-phenylbutyrate, a specific inhibitor of ER stress, up-regulation of EDA+ FN could be abrogated. Moreover, silencing CHOP by shRNA enhanced the release of EDA+ FN from hepatocytes following palmitate treatment, which was involved in ER stress-related cell damage. These findings suggest that the up-regulated level of EDA+ FN is associated with liver damage in NAFLD, and ER stress-mediated cell damage contributes to the release of EDA+ FN from hepatocytes. © 2017, Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： Yu, Yang*;Xiao, Jifen;Zhu, Wenjun;Yang, Yuheng;Mei, Jiaqin;...

期刊： Molecular Plant Pathology,2017年18(8):1052-1061 ISSN：1464-6722

通讯作者： Yu, Yang

作者机构： [Yu, Yang; Xiao, Jifen; Tan, Wanzhong; Qing, Ling; Yang, Yuheng; Bi, Chaowei] Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China.;[Zhu, Wenjun] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Mei, Jiaqin; Qian, Wei] Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China.

通讯机构： [Yu, Yang] S;Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China.

关键词： appressorium;Rhs repeat;sclerotia;Sclerotinia sclerotiorum;secretory protein;virulence

摘要： Sclerotinia sclerotiorum is a devastating necrotrophic plant pathogen with worldwide distribution. Cell wall-degrading enzymes and oxalic acid are important to the virulence of this pathogen. Here, we report a novel secretory protein Ss-Rhs1 that is essential for the virulence of S. sclerotiorum. Ss-Rhs1 is believed to contain a typical signal peptide at the N-terminal and eight rearrangement hotspot (Rhs) repeats. Ss-Rhs1 exhibited high-level expression at the initial stage of sclerotial development as well as during the hyphal infection process. Targeted silencing of Ss-Rhs1 resulted in abnormal colony morphology and reduced virulence on host plants. Microscopic observations indicated that Ss-Rhs1 silenced strains exhibited reduced efficiency in compound appressoria formation. This article is protected by copyright. All rights reserved.

语种： 英文

作者： Wang, Hualin;Shao, Yang;Yuan, Fahu;Feng, Han;Li, Na;...

期刊： BioMed Research International,2017年2017:2503847 ISSN：2314-6133

通讯作者： Liu, Zhiguo

作者机构： [Wang, Hualin; Feng, Han; Yuan, Fahu; Liu, Zhiguo; Li, Na; Shao, Yang; Zhang, Hongyu] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Yuan, Fahu] Jianghan Univ, Sch Med, Wuhan 430000, Hubei, Peoples R China.;[Wu, Chaodong] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA.

通讯机构： [Liu, Zhiguo] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

摘要： Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver diseases worldwide. Recent studies have indicated that fish oil supplementation has benefits against NAFLD. Our previous transcriptomic study has validated the effect of fish oil supplementation on altering hepatic gene expression in a NAFLD rat model. In the current study, we examined the effects of fish oil on the expression of hepatic microRNAs. Male Sprague-Dawley rats were fed with a lab chow (CON), high-fat high-cholesterol diet (WD), or WD supplemented with fish oil (FOH), respectively. Small RNAs were extracted from livers for RNA-sequencing. A total of 79 miRNAs were identified as differentially expressed miRNAs (DEMs) between FOH and WD groups, exemplified by rno-miR-29c-3p, rno-miR-30d-5p, rno-miR-33-5p, rno-miR-34a, and rno-miR-328a-3p. Functional annotation of DEMs predicted target genes suggested that the altered hepatic miRNAs contributed to fish oil modification of hepatic lipid metabolism and signaling transduction. Integrative analysis of DEMs and differentially expressed genes suggested that the expression difference of Pcsk9, Insig2, Per3, and Socs1/3 between FOH and WD groups may be due to miRNA modification. Our study reveals that fish oil supplementation alters hepatic expression of miRNAs, which may contribute to fish oil amelioration of NAFLD in rats. © 2017 Hualin Wang et al.

语种： 英文

作者： Yao, Xiangyang*;Lu, Binyu;Lu, Chaotian;Bai, Qin;Yan, Dazhong;...

期刊： FOOD & FUNCTION,2017年8(1):132-141 ISSN：2042-6496

通讯作者： Yao, Xiangyang

作者机构： [Yao, Xiangyang; Bai, Qin; Xu, Hui; Lu, Chaotian; Wu, Yanli; Hong, Zibing] Bengbu Univ, Dept Biol & Food Engn, Bengbu, Peoples R China.;[Lu, Binyu] Fudan Univ, Sch Pharm, Shanghai, Peoples R China.;[Yan, Dazhong] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Peoples R China.

通讯机构： [Yao, Xiangyang] B;Bengbu Univ, Dept Biol & Food Engn, Bengbu, Peoples R China.

摘要： The aim of the present study was to examine the anti-inflammatory effect of solanesol and to elucidate the underlying mechanisms. Heme oxygenase-1 (HO-1) plays an important role in cytoprotection against oxidative stress and inflammation. Solanesol induced HO-1 expression both at the level of mRNA and proteins, resulting in increased HO-1 activity. Solanesol treatment enhanced the level of the phosphorylated form, nuclear translocation, ARE-binding, and transcriptional activity of Nrf2. p38 and Akt contributed to ARE-driven HO-1 expression. Solanesol activated both p38 and Akt, and treatments with SB203580 (a p38 kinase inhibitor), LY294002 (an Akt inhibitor), specific p38 siRNA and Akt siRNA suppressed the solanesol-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Solanesol also elevated the autophagic protein LC3B-II level. SnPP (a HO-1 inhibitor) and HO-1 siRNA markedly abolished the anti-inflammatory effect of solanesol against LPS-induced cell damage. Likewise, SB203580, LY294002, 3-MA and Baf-A1 inhibited the solanesol-induced anti-inflammatory effect. These studies demonstrate that solanesol attenuates inflammation by HO-1 induction via p38 and Akt signaling. Thus, it is quite plausible that HO-1 induction by solanesol could trigger anti-inflammatory pathways including limiting LPS-stimulated cytokine production through autophagic signaling via p38 and Akt. © 2017 The Royal Society of Chemistry.

语种： 英文

作者： Zhao, Xiu-Ju*（赵秀举）;Chen, Yu-Lian;Fu, Bing;Zhang, Wen;Liu, Zhiguo*;...

期刊： Journal of the Science of Food and Agriculture,2017年97(4):1158-1163 ISSN：0022-5142

通讯作者： Zhao, Xiu-Ju（赵秀举）;Liu, Zhiguo

作者机构： [Zhao, Xiu-Ju; Liu, Zhiguo; Chen, Yu-Lian] Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Fu, Bing] Changyuan Cuisine Vocat & Tech Coll, Changyuan 453400, Peoples R China.;[Zhang, Wen] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.;[Zhuo, Hexian] Xinxiang Inst Drug Control Food & Drug Adm Xinxia, 17 Jiankang Rd, Xinxiang 453000, Peoples R China.

通讯机构： [Zhao, XJ; Liu, ZG] W;Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.

关键词： pumpkin seed oil (PSO);metabolic disease (MD);metabonomics;transcript profile

摘要： BACKGROUND: Understanding the metabolic and transcription basis of pumpkin seed oil (PSO) intervention on metabolic disease (MD) is essential to daily nutrition and health. RESULTS: This study analyzed the liver metabolic variations of Wistar rats fed normal diet (CON), high-fat diet (HFD) and high-fat plus PSO diet (PSO) to establish the relationship between the liver metabolite composition/transcript profile and the effects of PSO on MD. By using proton nuclear magnetic resonance spectroscopy together with multivariate data analysis, it was found that, compared with CON rats, HFD rats showed clear dysfunctions of choline metabolism, glucose metabolism and nucleotide and amino acid metabolism. Using quantitative real-time polymerase chain reaction (qPCR), it was found that, compared with HFD rats, PSO rats showed alleviated endoplasmic reticulum stress accompanied by lowered unfolded protein response. CONCLUSION: These findings provide useful information to understand the metabolic alterations triggered by MD and to evaluate the effects of PSO intervention. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry

语种： 英文

作者： Dai, Jianghong*

期刊： ENVIRONMENTAL MICROBIOLOGY REPORTS,2017年9(3):203-210 ISSN：1758-2229

通讯作者： Dai, Jianghong

作者机构： [Dai, Jianghong] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 Xuefu Rd S, Wuhan 430023, Peoples R China.

通讯机构： [Dai, Jianghong] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 Xuefu Rd S, Wuhan 430023, Peoples R China.

摘要： Investigating the physical-chemical setting of early life is a challenging task. In this contribution, the author attempted to introduce a provocative concept from cosmology – cosmic microwave background (CMB), which is the residual thermal radiation from a hot early Universe – to the field. For this purpose, the author revisited a recently deduced biomarker, the 1,6-anhydro bond of sugars in bacteria. In vitro, the 1,6-anhydro bond of sugars reflects and captures residual thermal radiation in thermochemical processes and therefore is somewhat analogous to CMB. In vivo, the formation process of the 1,6-anhydro bond of sugars on the peptidoglycan of prokaryotic cell wall is parallel to in vitro processes, suggesting that the 1,6-anhydro bond is an ideal CMB-like analogue that suggests a hot setting for early life. The CMB-like 1,6-anhydro bond is involved in the life cycle of viruses and the metabolism of eukaryotes, underlying this notion. From a novel perspective, the application of the concept of the CMB to microbial ecology may give new insights into a hot environment, such as hydrothermal vents, supporting early life and providing hypotheses to test in molecular palaeontology. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd

语种： 英文

作者： Jia, Luqiang;Tu, Tingyong;Huai, Qiangqiang;Sun, Jiaowen;Chen, Shanshan;...

期刊： PLOS ONE,2017年12(10):e0184602- ISSN：1932-6203

通讯作者： Shi, Zhongping;Ding, Jian

作者机构： [Chen, Shanshan; Tu, Tingyong; Huai, Qiangqiang; Sun, Jiaowen; Shi, Zhongping; Ding, Jian; Jia, Luqiang] Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi, Jiangsu, Peoples R China.;[Li, Xin] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Shi, ZP; Ding, J] J;Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi, Jiangsu, Peoples R China.

关键词： Fermentation;Cell growth;Energy metabolism;Cell metabolism;Glycerol;Oxygen;Protein metabolism;Ethanol

摘要： In heterologous protein productions by P. pastoris, methanol induction is generally initiated when cell concentration reaches very high density. The alternative strategy by initiating methanol induction at lower cells concentration was also reported to be effective in easing DO control, reducing toxic by-metabolites accumulation and increasing targeted proteins titers. However, the methanol/energy regulation mechanisms are seldom reported. We theoretically analyzed the methanol/energy metabolisms in protein expression process with the strategies of initiating induction at higher or lower cells concentrations, using monellin production as a prototype. When initiating induction at lower cells concentration and controlling induction temperature at 30°C, monellin concentration reached the highest levels of 2.62~2.71 g/L, which was 2.5~4.9 fold of those obtained with the strategy of initiating induction at higher cells concentration. With the desired induction strategy, 1) carbon metabolism ratio directing into the precursors synthesis route for monellin production reached the highest level of 65%, carbon metabolism ratios towards to precursors synthesis and ATP regeneration routes were regulated at relatively balanced levels; 2) monellin synthesis was completely cell growth associated, with the largest associated coefficient and higher specific growth rate; 3) theoretical NADH (energy) utilization efficiency η was the highest, and η stayed high levels (≥0.8) during most period (89%) within induction phase to supply sufficient energy in supporting monellin synthesis. © 2017 Jia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

语种： 英文

作者： Bai, Ding-Ping;Zhang, Xi-Feng;Zhang, Guo-Liang;Huang, Yi-Fan;Gurunathan, Sangiliyandi*

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2017年12:6521-6535 ISSN：1178-2013

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Bai, Ding-Ping; Huang, Yi-Fan] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou, Fujian, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Zhang, Guo-Liang] Dong E E Jiao Co Ltd, Liaocheng, Shandong, Peoples R China.;[Zhang, Guo-Liang] Natl Engn Res Ctr Gelatin Based Tradit Chinese Me, Liaocheng, Shandong, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

关键词： zinc oxide nanoparticles;human ovarian cancer cells SKOV3;mitochondrial membrane potential;apoptosis;DNA fragmentation;autophagy

摘要： Background: Zinc oxide nanoparticles (ZnO NPs) are frequently used in industrial products such as paint, surface coating, and cosmetics, and recently, they have been explored in biologic and biomedical applications. Therefore, this study was undertaken to investigate the effect of ZnO NPs on cytotoxicity, apoptosis, and autophagy in human ovarian cancer cells (SKOV3). Methods: ZnO NPs with a crystalline size of 20 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The cytotoxicity apoptosis, and autophagy were examined using a series of cellular assays. Results: Exposure of cells to ZnO NPs resulted in a dose-dependent loss of cell viability, and the characteristic apoptotic features such as rounding and loss of adherence, enhanced reactive oxygen species generation, and loss of mitochondrial membrane potential were observed in the ZnO NP-treated cells. Furthermore, the cells treated with ZnO NPs showed significant double-strand DNA breaks, which are gained evidences from significant number of γ-H2AX and Rad51 expressed cells. ZnO NP-treated cells showed upregulation of p53 and LC3, indicating that ZnO NPs are able to upregulate apoptosis and autophagy. Finally, the Western blot analysis revealed upregulation of Bax, caspase-9, Rad51, γ-H2AX, p53, and LC3 and downregulation of Bcl-2. Conclusion: The study findings demonstrated that the ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in human ovarian cells through reactive oxygen species generation and oxidative stress. Therefore, this study suggests that ZnO NPs are suitable and inherent anticancer agents due to their several favorable characteristic features including favorable band gap, electrostatic charge, surface chemistry, and potentiation of redox cycling cascades. © 2017 Bai et al.

语种： 英文

作者： Cai, Yan;Huang, Tingxuan;Xu, Yuekang;Zhou, Guoping;Zou, Ping;...

期刊： Archives of Microbiology,2017年199(5):775-785 ISSN：0302-8933

通讯作者： Liu, Xiaojin

作者机构： [Zeng, Guifeng; Cai, Yan; Zou, Ping] Changzhou Univ, Sch Food Sci & Technol, 1 Gehu Middle Rd, Changzhou 213164, Jiangsu, Peoples R China.;[Liu, Xiaojin; Xu, Yuekang] Anhui Normal Univ, Coll Life Sci, 1 Beijing East Rd, Wuhu 241002, Anhui, Peoples R China.;[Huang, Tingxuan; Zhou, Guoping] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

通讯机构： [Liu, Xiaojin] A;Anhui Normal Univ, Coll Life Sci, 1 Beijing East Rd, Wuhu 241002, Anhui, Peoples R China.

关键词： Bacillus;NheABC;Genetic diversity;MLST

摘要： Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurrence of NHE among them, and other members of Bacillus is unclear. Consequently, we aimed to determine the distribution and evolution of NHE within Bacillus by confirming the presence of the nheA, B and C sequences and variation within them using published data, and to analyze the genomic and genetic diversity. The phylogenetic tree of NHE proteins (NheA, NheB and NheC) from 81 different B. cereus s.l. strains was constructed. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis. Analysis of the genomic diversity of the nheA, B and C loci revealed that their upstream regions were more conserved than the downstream sequences. Multilocus sequence typing schemes (MLST) based on seven concatenated housekeeping genes and nheA, B and C genes of the 75 strains were developed. The neighbor joining phylogenetic tree based on seven housekeeping genes together with nheA, B and C genes was similiar to published Bacillus phylogenetic trees. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis.The results indicate that nheA, B and C genes do not affect the diversity of housekeeping genes, and this specific NHE protein does not participate in the diarrheic syndrome. © 2017, The Author(s).

语种： 英文