鄂西北地区猪场不同免疫水平下PCV-2的流行病学调查
作者：
潘奕凡;周华林;方聪;刘忠柱;李凡;...
期刊：
黑龙江畜牧兽医 ,2021年(03):86-90 ISSN：1004-7034
作者机构：
[熊江林; 潘奕凡; 刘忠柱; 吴灵英; 邱银生; 方聪; 李凡] 武汉轻工大学动物科学与营养工程学院/农副产品蛋白质饲料资源教育部工程研究中心;襄阳职业技术学院农学院/襄阳市动物医学工程技术研究中心;襄阳求是动物科技有限公司;[周华林] 襄阳职业技术学院农学院/襄阳市动物医学工程技术研究中心<&wdkj&>襄阳求是动物科技有限公司
关键词：
鄂西北;猪场;猪圆环病毒2型;免疫;流行病;调查
摘要：
为了解鄂西北地区猪场猪圆环病毒2型（Porcine circovirus type 2,PCV-2）不同免疫水平下的流行病学特点,试验采用ELISA法和PCR法分别对2017—2019年鄂西北地区猪场送检的1 456份血样和134份临床疑似PCV-2感染的病料进行了PCV-2抗体和病原检测,并统计采样猪的PCV-2疫苗免疫情况。根据2017—2019年采样猪PCV-2疫苗免疫记录,分别统计不同年份PCV-2疫苗免疫密度,分析不同免疫密度下PCV-2抗体水平差异。再根据采样猪的PCV-2疫苗免疫记录将猪分为未免疫、1次免疫及≥2次免疫共3个不同免疫频次组,分析不同免疫频次下PCV-2抗体阳性率和PCV-2病原阳性率差异。结果表明:2017年、2018年和2019年PCV-2疫苗免疫密度分别为91.2%、49.3%和39.9%,PCV-2抗体阳性率分别为78.1%、39.1%和34.8%,PCV-2疫苗免疫密度下降导致PCV-2抗体阳性率显著下降（P＜0.05）;未免疫、1次免疫和≥2次免疫的PCV-2抗体阳性率分别为32.4%、66.0%和86.5%,PCV-2病原阳性率分别为73.7%、42.0%和18.5%,PCV-2疫苗免疫频次增加能显著提高PCV-2抗体阳性率（P＜0.05）,显著降低PCV-2病原阳性率（P＜0.05）。说明鄂西北地区PCV-2防控形势严峻,增加免疫密度和加强免疫频次可提高PCV-2抗体水平,降低猪只感染PCV-2病原的风险。
语种：
中文
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Prevalence of aflatoxin M1 in raw milk and three types of liquid milk products in central-south China
作者：
Xiong, Jianglin;Peng, Lijuan;Zhou, Hualin;Lin, Bo;Yan, Pingyu;...
期刊：
Food Control ,2020年108:106840 ISSN：0956-7135
通讯作者：
Wu, Lingying
作者机构：
[Liu, Yulan; Wu, Lingying; Xiong, Jianglin; Qiu, Yinsheng; Yan, Pingyu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China.;[Lin, Bo] Guangxi Univ, Coll Anim Sci, Nanning 530004, Peoples R China.;[Wu, Wenxuan] Guizhou Univ, Coll Anim Sci, Guiyang 550025, Guizhou, Peoples R China.
通讯机构：
[Wu, Lingying] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Aflatoxin M1;China;Milk products;Raw milk
摘要：
The objective of this study was to survey the prevalence of aflatoxin M1 (AFM1) in raw, pasteurized, extended shelf life (ESL) and UHT milk from central-south China. A total of 734 milk samples, comprising raw milk (133 samples), pasteurized milk (410), ESL milk (93) and UHT milk (98), were obtained between Nov. 01, 2018 and Mar. 31, 2019. The AFM1 concentrations in milk samples were detected by ELISA and confirmed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Five hundred and ninety samples (80.4%) were positive for AFM1; the mean value was 23.7 ng/L, and the range was 5.1–104.4 ng/L. The percentages of AFM1 positive raw, pasteurized, ESL and UHT milk samples were 75.2, 82.2, 81.7 and 78.6%, respectively. The AFM1 concentration in raw milk was significantly lower (p < 0.05) than that in pasteurized and ESL milk, and the AFM1 level in UHT milk tended to be lower (p < 0.10) than that in pasteurized and ESL milk. The AFM1 levels exceeded the EU legal limit of 50 ng/L in 0, 11.5, 18.3 and 3.1% of raw, pasteurized, ESL and UHT milk samples, respectively. All milk samples tested had levels far below China's legal limit of 500 ng/L. Therefore, the AFM1 content in raw milk and milk products in central-south China does not pose a public health threat, but close attention should nonetheless be paid to milk AFM1, given the high positive rate of 80.4%. © 2019
语种：
英文
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“新农科”背景下卓越动物科学专业“晋级式”实践教学模式的研究与实践
作者：
熊江林;邱银生;吴灵英
期刊：
湖北畜牧兽医 ,2020年41(11):38-41 ISSN：1007-273X
作者机构：
武汉轻工大学动物科学与营养工程学院,武汉 430023;[吴灵英; 邱银生; 熊江林] 武汉轻工大学
关键词：
“新农科”背景;动物科学专业;“晋级式”实践教学模式
摘要：
近年来,国内养殖行业发展迅速,对高技能人才需求极大,而动物科学专业学生由于对行业认知不够,实践动手能力较差,进入企业需要连续培训,融入行业时间变长。课题组在“新农科”建设背景下,首次提出了“晋级式”实践教学模式,即采用企业人事制度,设置3个等级:技术员(初级)、技术经理(中级)和技术总经理(高级),并设定相应技术考核要求,学员进行初级实践学习并达到所有技术要求,才能晋升中级、高级开展实践学习和考核,逐级晋升考核。课题组对该模式的目标、原则和实施思路进行了剖析,并以复合应用人才的实践教学培养为例,研究“晋级式”实践教学模式在动物科学专业人才培养中的应用评价,实施效果较好。
语种：
中文
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Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion
作者：
Fu, Shulin;Zhao, Wenhua;Xiong, Chunhong;Guo, Ling;Guo, Jing;...
期刊：
INNATE IMMUNITY ,2019年25(7):420-432 ISSN：1753-4259
通讯作者：
Qiu, Yinsheng;Hu, Chien-An Andy
作者机构：
[Hu, Chien-An Andy; Liu, Yu; Zhao, Wenhua; Hou, Yongqing; Qiu, Yinsheng; Ye, Chun; Wu, Zhongyuan; Fu, Shulin; Xiong, Chunhong; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, YS; Hu, CAA] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Baicalin;Haemophilus parasuis;apoptosis;vascular endothelial cells;invasion
摘要：
Glässer’s disease, caused by Haemophilus parasuis, is a chronic disease related to an inflammatory immune response. Baicalin exerts important biological functions. In this study, we explored the protective efficacy of treatment with baicalin and the potential mechanism of activation of the MAPK signaling pathway in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis. H. parasuis stimulated expression of receptor for advanced glycation end products, induced a significant increase in the level of protein kinase-α and protein kinase-δ phosphorylation, and significantly up-regulated ERK, c-Jun N-terminal kinase, and p38 phosphorylation in PAVECs. H. parasuis also up-regulated the levels of apoptotic genes (Bax, C-myc, and Fasl) and the expression levels of c-Jun and c-Fos, and induced S-phase arrest in PAVECs. However, treatment with baicalin inhibited expression of RAGE, suppressed H. parasuis-induced protein kinase-α and protein kinase-δ phosphorylation, reduced ERK, c-Jun N-terminal kinase, and p38 phosphorylation, down-regulated apoptotic genes (Bax, C-myc, and Fasl), attenuated phospho-c-Jun production from the extracellular to the nuclei, and reversed S-phase arrest in PAVECs. In conclusion, baicalin treatment inhibited the MAPK signaling pathway, thereby achieving its anti-inflammatory responses, which provides a new strategy to control H. parasuis infection. © The Author(s) 2019.
语种：
英文
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Effects of Baicalin on piglet monocytes involving PKC-MAPK signaling pathways induced by Haemophilus parasuis.
作者：
Ye, Chun;Li, Ruizhi;Xu, Lei;Qiu, Yinsheng* ;Fu, Shulin;...
期刊：
BMC Veterinary Research ,2019年15(1):1-11 ISSN：1746-6148
通讯作者：
Qiu, Yinsheng
作者机构：
[Ye, Chun; Li, Ruizhi; Xu, Lei; Qiu, Yinsheng; Fu, Shulin; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China;[Ye, Chun; Qiu, Yinsheng; Fu, Shulin; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China. Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Apoptosis;Baicalin;Glasser's disease;Haemophilus parasuis;PKC-MAPK
摘要：
BACKGROUND: Haemophilus parasuis (HPS) is the causative agent of Glasser's disease, characterized by arthritis, fibrinous polyserositis and meningitis, and resulting in worldwide economic losses in the swine industry. Baicalin (BA), a commonly used traditional Chinese medication, has been shown to possess a series of activities, such as anti-bacterial, anti-viral, anti-tumor, anti-oxidant and anti-inflammatory activities. However, whether BA has anti-apoptotic effects following HPS infection is unclear. Here, we investigated the anti-apoptotic effects and mechanisms of BA in HPS-induced apoptosis via the protein kinase C (PKC)-mitogen-activated protein kinase (MAPK) pathway in piglet's mononuclear phagocytes (PMNP). RESULTS: Our data demonstrated that HPS could induce reactive oxygen species (ROS) production, arrest the cell cycle and promote apoptosis via the PKC-MAPK signaling pathway in PMNP. Moreover, when BA was administered, we observed a reduction in ROS production, suppression of cleavage of caspase-3 in inducing apoptosis, and inhibition of activation of the PKC-MAPK signaling pathway for down-regulating p-JNK, p-p38, p-ERK, p-PKC-alpha and PKC-delta in PMNP triggered by HPS. CONCLUSIONS: Our data strongly suggest that BA can reverse the apoptosis initiated by HPS through regulating the PKC-MAPK signaling pathway, which represents a promising therapeutic agent in the treatment of HPS infection.
语种：
英文
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Effect of baicalin-aluminum complexes on fecal microbiome in piglets
作者：
Fu, Shulin;Zhuang, Feng;Guo, Ling;Qiu, Yinsheng* ;Xiong, Jianglin;...
期刊：
International Journal of Molecular Sciences ,2019年20(10):2390 ISSN：1661-6596
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Yu; Xiong, Jianglin; Zhuang, Feng; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Xiong, Jianglin; Zhuang, Feng; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
ABC transporter;aluminum;baicalin;genomic DNA;hydrogenase;nicotinamide nucleotide adenylyltransferase;phosphotransferase;aluminum;antiinfective agent;baicalin;flavonoid;animal experiment;antimicrobial activity;Article;binding site;bioinformatics;biosynthesis;cell adhesion;chemotaxis;controlled study;diarrhea;discriminant analysis;DNA extraction;DNA library;DNA purification;DNA sequence;drug efficacy;enzyme activity;feces analysis;gene expression;gene ontology;metagenomics;microbial community;microbiome;nonhuman;phylogeny;principal component analysis;Scutellaria baicalensis;sequence alignment;sequence analysis;animal;chemistry;diarrhea;drug effect;feces;intestine flora;microbiology;pig;swine disease;veterinary medicine;Aluminum;Animals;Anti-Infective Agents;Diarrhea;Feces;Flavonoids;Gastrointestinal Microbiome;Swine;Swine Diseases
摘要：
The gut microbiome has important effects on gastrointestinal diseases. Diarrhea attenuation functions of baicalin (BA) is not clear. Baicalin–aluminum complexes (BBA) were synthesized from BA, but the BBA’s efficacy on the diarrhea of piglets and the gut microbiomes have not been explored and the mechanism remains unclear. This study has explored whether BBA could modulate the composition of the gut microbiomes of piglets during diarrhea. The results showed that the diarrhea rate reduced significantly after treatment with BBA. BBA altered the overall structure of the gut microbiomes. In addition, the Gene Ontology (GO) enrichment analysis indicated that the functional differentially expressed genes, which were involved in the top 30 GO enrichments, were associated with hydrogenase (acceptor) activity, nicotinamide-nucleotide adenylyltransferase activity, and isocitrate lyase activity, belong to the molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that flagellar assembly, bacterial chemotaxis, lipopolysaccharide biosynthesis, ATP-binding cassette transporters (ABC) transporters, biosynthesis of amino acids, and phosphotransferase system (PTS) were the most enriched during BBA treatment process. Taken together, our results first demonstrated that BBA treatment could modulate the gut microbiomes composition of piglets with diarrhea, which may provide new potential insights on the mechanisms of gut microbiomes associated underlying the antimicrobial efficacy of BBA. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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2,4-dinitrotoluene (Dnt) perturbs yolk absorption, liver development and lipid metabolism/oxygen transport gene expression in zebrafish embryos and larvae
作者：
Xiong, Jianglin;Sha, Hang;Zhou, Hualin;Peng, Lijuan;Wu, Lingying;...
期刊：
International Journal of Molecular Sciences ,2019年20(15):3632 ISSN：1661-6596
通讯作者：
Wang, Rui
作者机构：
[Hu, Xianqin; Wu, Lingying; Xiong, Jianglin; Qiu, Yinsheng; Wang, Rui] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Sha, Hang] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.;[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China.;[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Wang, Rui] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
2,4 dinitrotoluene;acyl coenzyme A oxidase;apolipoprotein A2;fatty acid binding protein;green fluorescent protein;heme oxygenase;hypoxia inducible factor 1alpha;microsomal triglyceride transfer protein;peroxisome proliferator activated receptor alpha;peroxisome proliferator activated receptor gamma;transferrin;2,4-dinitrotoluene;nitrobenzene derivative;oxygen;adult;animal experiment;animal model;animal tissue;Article;breathing;breeding;controlled study;down regulation;embryo;embryo development;fish breeding;gene expression;genetic transcription;larva;LC50;lipid metabolism;lipid transport;lipolysis;liver development;medical parameters;morphometry;mortality;nonhuman;oxygen transportation;polymerase chain reaction;post fertilization;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA isolation;stereomicroscopy;upregulation;yolk absorption;zebra fish;animal;drug effect;embryology;gene expression regulation;larva;lipid metabolism;liver;metabolism;nonmammalian embryo;organogenesis;pharmacology;pollutant;toxicity;transport at the cellular level;Animals;Biological Transport;Dinitrobenzenes;Embryo, Nonmammalian;Embryonic Development;Environmental Pollutants;Gene Expression Regulation, Developmental;Larva;Lipid Metabolism;Lipolysis;Liver;Organogenesis;Oxygen;Zebrafish
摘要：
2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. The results showed that the 120-h post-fertilization LC50 of 2,4-DNT was 9.59 mg/L with a 95% confidence interval of 8.89–10.44 mg/L. The larvae treated with 2,4-DNT showed toxic symptoms including smaller body, less skin pigment production, yolk malabsorption, and disordered liver development. Further studies on the expression of genes related to lipid transport and metabolism, and respiration indicated that they were significantly affected by 2,4-DNT. It is concluded that 2,4-DNT exposure perturbed liver development and yolk absorption in early-life zebrafish, and disturbed the lipid metabolism /oxygen transport gene expression. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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英文
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Tilmicosin enteric granules and premix to pigs: Antimicrobial susceptibility testing and comparative pharmacokinetics
作者：
Xiong, Jincheng;Zhu, Qianqian;Zhao, Yaxin;Yang, Shuaike;Cao, Jiyue* ;...
期刊：
Journal of Veterinary Pharmacology and Therapeutics ,2019年42(3):336-345 ISSN：0140-7783
通讯作者：
Cao, Jiyue;Qiu, Yinsheng
作者机构：
[Zhu, Qianqian; Zhao, Yaxin; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Coll Vet Med, Dept Vet Pharmacol, Wuhan, Hubei, Peoples R China.;[Zhu, Qianqian; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Zhu, Qianqian; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Qiu, Yinsheng] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Cao, Jiyue] H;[Qiu, Yinsheng] W;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan, Hubei, Peoples R China.;Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
bioavailability;pharmacokinetics;pig;susceptibility;tilmicosin
摘要：
The purpose of this study was to compare the pharmacokinetics and relative bioavailability of tilmicosin enteric granules and premix after oral administration at a dose of 40 mg/kg in pigs. Three kinds of different respiratory pathogens were selected for determination of minimal inhibitory concentration (MIC) to tilmicosin. Eight healthy pigs were assigned to a two-period, randomized crossover design. A modified rapid, sensitive HPLC method was used for determining the concentrations of tilmicosin in plasma. Pharmacokinetic parameters were calculated by using WinNonlin 5.2 software. The MIC90 of tilmicosin against Haemophilus parasuis, Actinbacillus pleuropneumoniae, and Pasteurella multocida were all 8 mug/ml. These results indicated that these common pig respiratory bacteria are sensitive to tilmicosin. The main parameters of time to reach maximum plasma concentration (Tmax ), elimination half-life (t1/2beta ), mean residence time (MRT), and apparent volume of distribution (VF ) were 2.03 +/- 0.37 hr, 29.31 +/- 5.56 hr, 25.22 +/- 2.57 hr, 4.06 +/- 1.04 L/kg, and 3.05 +/- 0.08 hr, 17.06 +/- 1.77 hr, 15.55 +/- 1.37 hr, 2.95 +/- 0.62 L/kg after the orally administrated tilmicosin enteric granules and premix. The relative bioavailability of tilmicosin enteric granules to premix was 114.97 +/- 7.19%, according to the AUC0-t values. These results demonstrated that tilmicosin enteric granules produced faster tilmicosin absorption, slower elimination, larger tissue distribution, and higher bioavailability compared to the tilmicosin premix. The present study results manifest that tilmicosin enteric granules can be used as a therapeutic alternative to premix in clinical treatment.
语种：
英文
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Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets
作者：
Liu, Yu;Zhao, Wenhua;Xu, Jianfeng;Yu, Xiaolin;Ye, Chun;...
期刊：
Journal of Veterinary Pharmacology and Therapeutics ,2019年42(5):580-584 ISSN：0140-7783
通讯作者：
Qiu, Yinsheng
作者机构：
[Liu, Yu; Zhao, Wenhua; Xu, Jianfeng; Yu, Xiaolin; Ye, Chun; Fu, Shulin; Qiu, Yinsheng] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China
通讯机构：
[Qiu, Yinsheng] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
baicalin;antiinfective agent;baicalin;flavonoid;animal experiment;area under the curve;Article;drug absorption;drug bioavailability;drug blood level;drug clearance;drug distribution;drug elimination;drug half life;female;male;nonhuman;pharmacokinetics;piglet;plasma concentration-time curve;priority journal;animal;blood;half life time;intramuscular drug administration;intravenous drug administration;pig;Animals;Anti-Infective Agents;Area Under Curve;Flavonoids;Half-Life;Injections, Intramuscular;Injections, Intravenous;Swine
摘要：
The purpose of this study was to determine the pharmacokinetics of baicalin after intravenous and intramuscular administration of sodium baicalin at 50 mg/kg to piglets. Plasma baicalin levels were determined by high-performance liquid chromatography. The plasma concentration-time data of baicalin for both administration routes were best described by two-compartmental open model. The area under the plasma concentration-time curve and the elimination half-lives were 77.47 +/- 6.14 mu g/ml x h and 1.73 +/- 0.16 hr for intravenous and 64.85 +/- 5.67 mu g/ml x h and 2.42 +/- 0.15 hr for intramuscular administration, respectively. The apparent volume of distribution and body clearance were 1.63 +/- 0.23 L/kg and 2.74 +/- 0.30 L h(-1) kg(-1) for intravenous and 0.51 +/- 0.10 L/kg and 0.78 +/- 0.08 L h(-1) kg(-1) for intramuscular routes, respectively. An intramuscular injection of sodium baicalin in piglets resulted in rapid and complete absorption, with a mean maximal plasma concentration of 77.28 +/- 7.40 mu g/ml at 0.17 hr and a high absolute bioavailability of 83.73 +/- 5.53%.
语种：
英文
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黄芩苷对副猪嗜血杆菌诱导后猪血管内皮细胞黏附分子表达的影响
作者：
徐健峰;郭靖;刘华山;付书林;邱银生
期刊：
武汉轻工大学学报 ,2019年38(1):17-21 ISSN：2095-7386
作者机构：
武汉轻工大学 动物科学与营养工程学院,湖北 武汉,430023;[徐健峰; 邱银生; 郭靖; 刘华山; 付书林] 武汉轻工大学
关键词：
黄芩苷;副猪嗜血杆菌;猪血管内皮细胞;细胞黏附分子
摘要：
为了探讨黄芩苷对副猪嗜血杆菌（HPS）诱导后猪血管内皮细胞黏附分子E-选择素（E-selectin）、细胞间黏附分子1（ICAM-1）和血管细胞黏附分子（VCAM-1）的影响,体外培养猪血管内皮细胞,接种副猪嗜血杆菌。用ELISA法检测细胞培养液中E-selectin、ICAM-1和VCAM-1释放量;用Q-RT PCR法检测血管内皮细胞内E-selectin、ICAM-1和VCAM-1的mRNA表达水平。结果表明:HPS感染猪血管内皮细胞后,细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平都极显著地上调（P <0. 01）;加入药物干预后,100μg/mL的黄芩苷能极显著地抑制细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平（P <0. 01）。实验表明HPS感染猪血管内皮细胞后可以激活细胞黏附分子,黄芩苷可以抑制细胞黏附分子的表达。
语种：
中文
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Baicalin modulates NF-kappa B and NLRP3 inflammasome signaling in porcine aortic vascular endothelial cells Infected by Haemophilus parasuis Causing Glasser's disease
作者：
Fu, Shulin;Liu, Huashan;Xu, Lei;Qiu, Yinsheng* ;Liu, Yu;...
期刊：
Scientific Reports ,2018年8(1):807 ISSN：2045-2322
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Huashan; Liu, Yu; Hou, Yongqing; Xu, Lei; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
摘要：
<jats:title>Abstract</jats:title><jats:p><jats:italic>Haemophilus parasuis (H. parasuis)</jats:italic> can cause vascular inflammatory injury, but the molecular basis of this effect remains unclear. In this study,we investigated the effect of the anti-inflammatory, anti-microbial and anti-oxidant agent, baicalin, on the nuclear factor (NF)-κB and NLRP3 inflammasome signaling pathway in pig primary aortic vascular endothelial cells. Activation of the NF-κB and NLRP3 inflammasome signaling pathway was induced in <jats:italic>H. parasuis</jats:italic>-infected cells. However, baicalin reduced the production of reactive oxygen species, apoptosis, and activation of the NF-κB and NLRP3 inflammasome signaling pathway in infected cells. These results revealed that baicalin can inhibit <jats:italic>H. parasuis</jats:italic>-induced inflammatory responses in porcine aortic vascular endothelial cells, and may thus offer a novel strategy for controlling and treating <jats:italic>H. parasuis</jats:italic> infection. Furthermore, the results suggest that piglet primary aortic vascular endothelial cells may provide an experimental model for future studies of <jats:italic>H. parasuis</jats:italic> infection.</jats:p>
语种：
英文
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PK-PD Integration Modeling and Cutoff Value of Florfenicol against Streptococcus suis in Pigs
作者：
Lei, Zhixin;Liu, Qianying;Yang, Shuaike;Yang, Bing;Khaliq, Haseeb;...
期刊：
Frontiers in Pharmacology ,2018年9(JAN):2 ISSN：1663-9812
通讯作者：
He, Qigai;Cao, Jiyue;Qiu, Yinsheng
作者机构：
[Yang, Bing; Liu, Qianying; Zhang, Bingzhou; Lei, Zhixin; He, Qigai; Khaliq, Haseeb; Ahmed, Saeed; Chen, Pin] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China.;[Yang, Bing; Liu, Qianying; Lei, Zhixin; Sajid, Abdul; Ahmed, Saeed; Chen, Pin; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Yang, Bing; Liu, Qianying; Lei, Zhixin; Sajid, Abdul; Ahmed, Saeed; Chen, Pin; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, MAO Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Lei, Zhixin; Li, Kun; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan, Hubei, Peoples R China.;[Li, Kun] Univ Illinois, Coll Vet Med, Champaign, IL USA.
通讯机构：
[He, Qigai; Cao, Jiyue] H;[Qiu, Yinsheng] W;Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China.;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan, Hubei, Peoples R China.;Huazhong Agr Univ, MAO Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.
关键词：
Streptococcus suis;florfenicol;optimal dosages;pharmacodynamic;pharmacokinetic
摘要：
The aims of the present study were to establish optimal doses and provide an alternate COPD for florfenicol against Streptococcus suis based on pharmacokinetic-pharmacodynamic integration modeling. The recommended dose (30 mg/kg b.w.) were administered in healthy pigs through intramuscular and intravenous routes for pharmacokinetic studies. The main pharmacokinetic parameters of Cmax, AUC0-24h, AUC, Ke, t1/2ke, MRT, Tmax, and Clb, were estimated as 4.44 μg/ml, 88.85 μg·h/ml, 158.56 μg·h/ml, 0.048 h-1, 14.46 h, 26.11 h, 4 h and 0.185 L/h·kg, respectively. The bioavailability of florfenicol was calculated to be 99.14% after I.M administration. A total of 124 Streptococcus suis from most cities of China were isolated to determine the minimum inhibitory concentration (MIC) of florfenicol. The MIC50 and MIC90 were calculated as 1 and 2 μg/ml. A serotype 2 Streptococcus suis (WH-2), with MIC value similar to MIC90, was selected as a representative for an in vitro and ex vivo pharmacodynamics study. The MIC values of WH-2 in TSB and plasma were 2 μg/ml, and the MBC/MIC ratios were 2 in TSB and plasma. The MPC was detected to be 3.2 μg/ml. According to inhibitory sigmoid Emax model, plasma AUC0-24h/MIC values of florfenicol versus Streptococcus suis were 37.89, 44.02, and 46.42 h for the bactericidal, bacteriostatic, and elimination activity, respectively. Monte Carlo simulations the optimal doses for bactericidal, bacteriostatic, and elimination effects were calculated as 16.5, 19.17, and 20.14 mg/kg b.w. for 50% target attainment rates (TAR), and 21.55, 25.02, and 26.85 mg/kg b.w. for 90% TAR, respectively. The PK-PD cutoffvalue (COPD) analyzed from MCS for florfenicol against Streptococcus suis was 1 μg/ml which could provide a sensitivity cutoffvalue. These results contributed an optimized alternative to clinical veterinary medicine and showed that the dose of 25.02 mg/kg florfenicol for 24 h could have a bactericidal action against Streptococcus suis after I.M administration. However, it should be validated in clinical practice in the future investigations. © 2018 Lei, Liu, Yang, Yang, Khaliq, Li, Ahmed, Sajid, Zhang, Chen, Qiu, Cao and He.
语种：
英文
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Baicalin inhibits Haemophilus Parasuis-induced high-mobility group box 1 release during inflammation
作者：
Fu, Shulin;Liu, Huashan;Chen, Xiao;Qiu, Yinsheng* ;Ye, Chun;...
期刊：
International Journal of Molecular Sciences ,2018年19(5):1307 ISSN：1661-6596
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Huashan; Liu, Yu; Hou, Yongqing; Ye, Chun; Chen, Xiao; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Huashan; Liu, Yu; Hou, Yongqing; Ye, Chun; Chen, Xiao; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
baicalin;high mobility group B1 protein;lipopolysaccharide;tumor necrosis factor;antiinfective agent;antiinflammatory agent;baicalin;cytokine;flavonoid;high mobility group B1 protein;animal cell;animal model;antiinflammatory activity;antimicrobial activity;Article;blood analysis;CCL4 gene;CCR2 gene;CD14 gene;CXCL9 gene;CYCS gene;enzyme linked immunosorbent assay;gene;gene sequence;Haemophilus parasuis;inflammation;monocyte;Myd88 gene;nonhuman;protein expression;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA extraction;RNA sequence;sequence analysis;STAT3 gene;Western blotting;animal;dose response;drug effect;genetics;Haemophilus infection;Haemophilus parasuis;inflammation;metabolism;nucleotide sequence;pathogenicity;pig;primary cell culture;swine disease;veterinary medicine;Animals;Anti-Infective Agents;Anti-Inflammatory Agents;Base Sequence;Cytokines;Dose-Response Relationship, Drug;Flavonoids;Haemophilus Infections;Haemophilus parasuis;HMGB1 Protein;Inflammation;Monocytes;Primary Cell Culture;Swine;Swine Diseases
摘要：
Haemophilus parasuis (H. parasuis) can cause Glässer’s disease in pigs. However, the molecular mechanism of the inflammation response induced by H. parasuis remains unclear. The high-mobility group box 1 (HMGB1) protein is related to the pathogenesis of various infectious pathogens, but little is known about whether H. parasuis can induce the release of HMGB1 in piglet peripheral blood monocytes. Baicalin displays important anti-inflammatory and anti-microbial activities. In the present study, we investigated whether H. parasuis can trigger the secretion of HMGB1 in piglet peripheral blood monocytes and the anti-inflammatory effect of baicalin on the production of HMGB1 in peripheral blood monocytes induced by H. parasuis during the inflammation response. In addition, host cell responses stimulated by H. parasuis were determined with RNA-Seq. The RNA-Seq results showed that H. parasuis infection provokes the expression of cytokines and the activation of numerous pathways. In addition, baicalin significantly reduced the release of HMGB1 in peripheral blood monocytes induced by H. parasuis. Taken together, our study showed that H. parasuis can induce the release of HMGB1 and baicalin can inhibit HMGB1 secretion in an H. parasuis-induced peripheral blood monocytes model, which may provide a new strategy for preventing the inflammatory disorders induced by H. parasuis. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Transcriptional profiling of host cell responses to virulent Haemophilus parasuis: New insights into pathogenesis
作者：
Fu, Shulin;Guo, Jing;Li, Ruizhi;Qiu, Yinsheng* ;Ye, Chun;...
期刊：
International Journal of Molecular Sciences ,2018年19(5):1320 ISSN：1661-6596
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Li, Ruizhi; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Li, Ruizhi; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
fibroblast growth factor 16;interleukin 7 receptor;phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase;Rap1 protein;serum and glucocorticoid regulated kinase 1;transcription factor FOXO;transcriptome;forkhead transcription factor;phosphatidylinositol 3 kinase;protein kinase B;transcriptome;arthritis;Article;ATF6B gene;bacterial gene;bacterial virulence;bioinformatics;blood vessel injury;cAMP signaling;controlled study;down regulation;endocytosis;F2R gene;FGF16 gene;gene cluster;gene overexpression;Haemophilus infection;Haemophilus parasuis;IL7R gene;meningitis;molecular genetics;next generation sequencing;nonhuman;nucleotide sequence;Pi3K/Akt signaling;PKN2 gene;pneumonia;polyserositis;PTEN gene;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA sequence;SGK1 gene;TLR signaling;transcriptomics;upregulation;animal;cell culture;cytology;endothelium cell;genetics;Haemophilus parasuis;metabolism;microbiology;pathogenicity;pig;vascular endothelium;Animals;Cells, Cultured;Endothelial Cells;Endothelium, Vascular;Forkhead Transcription Factors;Haemophilus Infections;Haemophilus parasuis;Phosphatidylinositol 3-Kinases;Proto-Oncogene Proteins c-akt;Swine;Transcriptome
摘要：
Haemophilus parasuis is the causative agent of Glässer’s disease in pigs. H. parasuis can cause vascular damage, although the mechanism remains unclear. In this study, we investigated the host cell responses involved in the molecular pathway interactions in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis using RNA-Seq. The transcriptome results showed that when PAVECs were infected with H. parasuis for 24 h, 281 differentially expressed genes (DEGs) were identified; of which, 236 were upregulated and 45 downregulated. The 281 DEGs were involved in 136 KEGG signaling pathways that were organismal systems, environmental information processing, metabolism, cellular processes, and genetic information processing. The main pathways were the Rap1, FoxO, and PI3K/Akt signaling pathways, and the overexpressed genes were determined and verified by quantitative reverse transcription polymerase chain reaction. In addition, 252 genes were clustered into biological processes, molecular processes, and cellular components. Our study provides new insights for understanding the interaction between bacterial and host cells, and analyzed, in detail, the possible mechanisms that lead to vascular damage induced by H. parasuis. This may lead to development of novel therapeutic targets to control H. parasuis infection. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Tea polyphenols suppress growth and virulence-related factors of Haemophilus parasuis
作者：
Guo, Ling;Guo, Jing;Liu, HuaShan;Zhang, Jing;Chen, Xiabing;...
期刊：
Journal of Veterinary Medical Science ,2018年80(7):1047-1053 ISSN：0916-7250
通讯作者：
Fu, Shulin
作者机构：
[Zhang, Jing; Liu, HuaShan; Qiu, Yinsheng; Fu, Shulin; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Chen, Xiabing] Wuhan Acad Agr Sci & Technol, Inst Anim Husb & Vet Sci, Wuhan 430208, Hubei, Peoples R China.
通讯机构：
[Fu, Shulin] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
polyphenol;virulence factor;animal;chemistry;growth, development and aging;Haemophilus infection;Haemophilus parasuis;pathogenicity;pig;swine disease;tea;veterinary medicine;virulence;Animals;Haemophilus Infections;Haemophilus parasuis;Polyphenols;Swine;Swine Diseases;Tea;Virulence;Virulence Factors
摘要：
The bacterium Haemophilus parasuis (H. parasuis) is the primary cause of Glässer’s disease. Currently, there are no effective vaccines that can confer protection against all H. parasuis serovars. Therefore, the present study aimed to investigate the effect of tea polyphenols on growth, expression of virulence-related factors, and biofilm formation of H. parasuis, as well as to evaluate their protective effects against H. parasuis challenge. Our findings demonstrated that tea polyphenols can inhibit H. parasuis growth in a dose-dependent manner and attenuate the biofilm formation of H. parasuis. In addition, tea polyphenols exerted inhibitory effects on the expression of H. parasuis virulence-related factors. Moreover, tea polyphenols could confer protection against a lethal dose of H. parasuis and can reduce pathological tissue damage induced by H. parasuis. In summary, our findings demonstrated the promising use of tea polyphenols as a novel treatment for H. parasuis infection in pigs. © 2018 The Japanese Society of Veterinary Science.
语种：
英文
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Bioequivalence evaluation of two 5% ceftiofur hydrochloride sterile suspension in pigs
作者：
Xiong, Jincheng;Zhu, Qianqian;Lei, Zhixin;Yang, Shuaike;Chen, Peiyuan;...
期刊：
Journal of Veterinary Medical Science ,2018年80(12):1847-1852 ISSN：0916-7250
通讯作者：
Cao, Jiyue;Qiu, Yinsheng
作者机构：
[Zhu, Qianqian; Zhao, Yaxin; Lei, Zhixin; Xiong, Jincheng; Yang, Shuaike; Chen, Peiyuan; Cao, Jiyue] Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China.;[Zhu, Qianqian; Zhao, Yaxin; Lei, Zhixin; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Zhu, Qianqian; Zhao, Yaxin; Lei, Zhixin; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Qiu, Yinsheng] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Cao, Jiyue] H;[Qiu, Yinsheng] W;Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.
关键词：
antiinfective agent;ceftiofur;cephalosporin derivative;animal;clinical trial;crossover procedure;drug formulation;female;high performance liquid chromatography;male;metabolism;pig;therapeutic equivalence;veterinary medicine;Animals;Anti-Bacterial Agents;Cephalosporins;Chromatography, High Pressure Liquid;Cross-Over Studies;Drug Compounding;Female;Male;Swine;Therapeutic Equivalency
摘要：
The purpose of this study was to evaluate the bioequivalence of 5% ceftiofur hydrochloride sterile suspension in two formulations, a test formulation (Saifukang 5% CEF, Hvsen) and a reference formulation (Excenel®RTU 5% CEF, Pfizer). Twenty-four healthy pigs were assigned to a two-period, two-treatment crossover parallel trial, and both formulations were administered at a single intramuscular dose of 5 mg/kg weight, with a 7-day washout period. Blood samples were collected consecutively for up to 144 hr after administration. The concentrations of ceftiofur-and desfuroylceftiofur-related metabolites in the plasma were determined by high-performance liquid chromatography. In addition, the major pharmacokinetic parameters (Cmax, AUC0-t and AUC0-∞) were computed and compared via analysis of variance, with 90% confidence intervals. Bioequivalence evaluation of Tmax was statistically analyzed with the nonparametric test. The comparison values between test and reference formulation for AUC0-t, AUC0-∞, Cmax, and Tmax were 376.7 ± 75.3 µg·hr/ml, 390.5 ± 78.6 µg·hr/ml, 385.9 ± 79.2 µg·hr/ml, 402.7 ± 80.4 µg·hr/ml, 34.6 ± 5.5 µg/ml, 36.1 ± 6.2 µg/ml, 1.27 ± 0.18 hr, and 1.26 ± 0.21 hr, respectively, and we observed no significant differences between the two formulations. The 90% CI values were within the recommended range of 80–125% (P>0.05), and the relative bioavailability of the test product was 96.47 ± 10.92% according to AUC0-t values. Based on our results, the two formulations exhibit comparable pharmacokinetic profiles, and the test product is bioequivalent to the reference formulation. © 2018 The Japanese Society of Veterinary Science.
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英文
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Roles of amino acids in preventing and treating intestinal diseases: recent studies with pig models
作者：
Liu, Yulan* ;Wang, Xiuying;Hou, Yongqing;Yin, Yulong;Qiu, Yinsheng;...
期刊：
Amino Acids ,2017年49(8):1277-1291 ISSN：0939-4451
通讯作者：
Liu, Yulan;Hu, Chien-An Andy
作者机构：
[Hu, Chien-An Andy; Liu, Yulan; Hou, Yongqing; Qiu, Yinsheng; Liu, YL; Hu, CAA; Wang, Xiuying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Hu, Chien-An Andy; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr & Hlth, Changsha 410125, Hunan, Peoples R China.;[Hu, Chien-An Andy; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol, Changsha 410125, Hunan, Peoples R China.;[Hu, Chien-An Andy; Yin, Yulong] Human Normal Univ, Inst Life Sci, Changsha 410081, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Liu, YL; Hu, CAA] W;[Hu, Chien-An Andy] C;[Hu, Chien-An Andy] H;[Hu, Chien-An Andy] U;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
Pig models;Intestinal disease;Amino acids;Therapeutics
摘要：
Animal models are needed to study and understand a human complex disease. Because of their similarities in anatomy, structure, physiology, and pathophysiology, the pig has proven its usefulness in studying human gastrointestinal diseases, such as inflammatory bowel disease, ischemia/reperfusion injury, diarrhea, and cancer. To understand the pathogenesis of these diseases, a number of experimental models generated in pigs are available, for example, through surgical manipulation, chemical induction, microbial infection, and genetic engineering. Our interests have been using amino acids as therapeutics in pig and human disease models. Amino acids not only play an important role in protein biosynthesis, but also exert significant physiological effects in regulating immunity, anti-oxidation, redox regulation, energy metabolism, signal transduction, and animal behavior. Recent studies in pigs have shown that specific dietary amino acids can improve intestinal integrity and function under normal and pathological conditions that protect the host from different diseases. In this review, we summarize several pig models in intestinal diseases and how amino acids can be used as therapeutics in treating pig and human diseases.
语种：
英文
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Evaluation of recombinant protein superoxide dismutase of Haemophilus parasuis strain SH0165 as vaccine candidate in a mouse model
作者：
Guo, Ling;Xu, Lei;Wu, Tao;Fu, Shulin* ;Qiu, Yinsheng* ;...
期刊：
CANADIAN JOURNAL OF MICROBIOLOGY ,2017年63(4):312-320 ISSN：0008-4166
通讯作者：
Fu, Shulin;Qiu, Yinsheng
作者机构：
[Wu, Tao; Hu, Chien-An Andy; Xu, Lei; Qiu, Yinsheng; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Ren, Xinglong] Huazhong Agr Univ, Coll Sci, Wuhan 430070, Peoples R China.;[Ye, Mengdie; Liu, Rongrong] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Fu, SL; Qiu, YS] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Haemophilus parasuis;Glässer’s disease;superoxide dismutase;vaccine;Haemophilus parasuis;maladie de Glässer;superoxyde dismutase;vaccin
摘要：
Haemophilus parasuis can cause a severe membrane inflammation disorder. It has been documented that superoxide dismutase (SOD) is a potential target to treat systemic inflammatory diseases. Therefore, we constructed an experimental H. parasuis subunit vaccine SOD and determined the protective efficacy of SOD using a lethal dose challenge against H. parasuis serovar 4 strain MD0322 and serovar 5 strain SH0165 in a mouse model. The results demonstrated that SOD could induce a strong humoral immune response in mice and provide significant immunoprotection efficacy against a lethal dose of H. parasuis serovar 4 strain MD0322 or serovar 5 strain SH0165 challenge. IgG subtype analysis indicated SOD protein could trigger a bias toward a Th1-type immune response and induce the proliferation of splenocytes and secretion of IL-2 and IFN-γ of splenocytes. In addition, serum in mice from the SOD-immunized group could inhibit the growth of strain MD0322 and strain SH0165 in the whole-blood killing bacteria assay. This is the first report that immunization of mice with SOD protein could provide protective effect against a lethal dose of H. parasuis serovar 4 and serovar 5 challenge in mice, which may provide a novel approach against heterogeneous serovar infection of H. parasuis in future. © 2017, Canadian Science Publishing. All rights reserved.
语种：
英文
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Comparative transcriptional profiling of tildipirosin-resistant and sensitive Haemophilus parasuis
作者：
Lei, Zhixin;Fu, Shulin;Yang, Bing;Liu, Qianying;Ahmed, Saeed;...
期刊：
Scientific Reports ,2017年7(1):7517 ISSN：2045-2322
通讯作者：
Cao, Jiyue;Qiu, Yinsheng
作者机构：
[Yang, Bing; Ahmed, Saeed; Liu, Qianying; Lei, Zhixin; Xiong, Jincheng; Cao, Jiyue] Huazhong Agr Univ, Coll Vet Med, Vet Pharmacol Lab, Wuhan 430070, Hubei, Peoples R China.;[Yang, Bing; Ahmed, Saeed; Liu, Qianying; Lei, Zhixin; Xiong, Jincheng; Cao, Jiyue] Huazhong Agr Univ, Nat Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Yang, Bing; Ahmed, Saeed; Liu, Qianying; Lei, Zhixin; Xiong, Jincheng; Cao, Jiyue] Huazhong Agr Univ, MAO Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Xu, Lei; Qiu, Yinsheng; Fu, Shulin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Cao, Jiyue] H;[Qiu, Yinsheng] W;Huazhong Agr Univ, Coll Vet Med, Vet Pharmacol Lab, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, Nat Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, MAO Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.
摘要：
Numerous studies have been conducted to examine the molecular mechanism of Haemophilus parasuis resistance to antibiotic, but rarely to tildipirosin. In the current study, transcriptional profiling was applied to analyse the variation in gene expression of JS0135 and tildipirosin-resistant JS32. The growth curves showed that JS32 had a higher growth rate but fewer bacteria than JS0135. The cell membranes of JS32 and a resistant clinical isolate (HB32) were observed to be smoother than those of JS0135. From the comparative gene expression profile 349 up- and 113 downregulated genes were observed, covering 37 GO and 63 KEGG pathways which are involved in biological processes (11), cellular components (17), molecular function (9), cellular processes (1), environmental information processing (4), genetic information processing (9) and metabolism (49) affected in JS32. In addition, the relative overexpression of genes of the metabolism pathway (HAPS_RS09315, HAPS_RS09320), ribosomes (HAPS_RS07815) and ABC transporters (HAPS_RS10945) was detected, particularly the metabolism pathway, and verified with RT-qPCR. Collectively, the gene expression profile in connection with tildipirosin resistance factors revealed unique and highly resistant determinants of H. parasuis to macrolides that warrant further attention due to the significant threat of bacterial resistance.
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英文
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Combination Susceptibility Testing of Common Antimicrobials in Vitro and the Effects of Sub-MIC of Antimicrobials on Staphylococcus aureus Biofilm Formation
作者：
Yang, Bing;Lop, Zhixin;Zhao, Yishuang;Ahmed, Saeed;Wang, Chunqun;...
期刊：
Frontiers in Microbiology ,2017年8(NOV):2125 ISSN：1664-302X
通讯作者：
Cao, Jiyue;Qiu, Yinsheng
作者机构：
[Yang, Bing; Zhao, Yishuang; Zhang, Shishuo; Lop, Zhixin; Wang, Chunqun; Cao, Jiyue] Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan, Hubei, Peoples R China.;[Yang, Bing; Ahmed, Saeed; Lop, Zhixin; Cao, Jiyue] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Yang, Bing; Ahmed, Saeed; Lop, Zhixin; Cao, Jiyue] Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.;[Qiu, Yinsheng; Fu, Shulin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan, Hubei, Peoples R China.
通讯机构：
[Cao, Jiyue] H;[Qiu, Yinsheng] W;Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan, Hubei, Peoples R China.;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan, Hubei, Peoples R China.;Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan, Hubei, Peoples R China.
关键词：
antimicrobial;biofilm formation;morphological observation;sub-MIC
摘要：
The current study was conducted to evaluate the antibacterial combination efficacies, and whether the sub-inhibitory concentrations (sub-MIC) of antibiotics can influent on the biofilm formation of S. aureus. The minimum inhibitory concentration (MIC) of common antibacterial drugs was determined in vitro against clinical isolates of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Pasteurella multocida (P. multocida) alone and in combination with each other by using the broth microdilution method and the checkerboard micro-dilution method analyzed with the fractional inhibitory concentration index (FICI), respectively. Regarding these results, antibacterial drug combinations were categorized as synergistic, interacting, antagonistic and indifferent, and most of the results were consistent with the previous reports. Additionally, the effects of sub-MIC of seven antimicrobials (kanamycin, acetylisovaleryltylosin tartrate, enrofloxacin, lincomycin, colistin sulfate, berberine, and clarithromycin) on S. aureus biofilm formation were determined via crystal violet staining, scanning electron microscopy (SEM) and real-time PCR. Our results demonstrate that all antibiotics, except acetylisovaleryltylosin tartrate, effectively reduced the S. aureus biofilm formation. In addition, real-time reverse transcriptase PCR was used to analyze the relative expression levels of S. aureus biofilm-related genes such as sarA, fnbA, rbf, lrgA, cidA, and eno after the treatment at sub-MIC with all of the six antimicrobials. All antibiotics significantly inhibited the expression of these biofilm-related genes except for acetylisovaleryltylosin tartrate, which efficiently up-regulated these transcripts. These results provide the theoretical parameters for the selection of effective antimicrobial combinations in clinical therapy and demonstrate how to correctly use antibiotics at sub-MIC as preventive drugs. © 2017 Yang, Lei, Zhao, Ahmed, Wang, Zhang, Fu, Cao and Qiu.
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英文
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