Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide
作者：
Xu, Xiao;Chen, Shaokui;Wang, Haibo;Tu, Zhixiao;Wang, Shuhui;...
期刊：
British Journal of Nutrition ,2018年119(9):1019-1028 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Xu, Xiao; Liu, Yulan; Chen, Shaokui; Wang, Haibo; Wang, Shuhui; Wang, Chunwei; Tu, Zhixiao; Wang, Xiuying; Zhu, Jiadong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Inflammatory response;Intestine;Medium-chain TAG;Necroptosis;Weanling piglets
摘要：
This study was conducted to evaluate whether medium-chain TAG (MCT) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial inflammatory response, as well as necroptosis. A total of twenty-four weanling piglets were randomly allotted to one of four treatments in a 2×2 factorial arrangement including diet type (5 % maize oil v. 4 % MCT+1 % maize oil) and immune stress (saline v. E. coli LPS). The piglets were fed diets containing maize oil or MCT for 21 d. On 21 d, piglets were injected intraperitoneally with saline or LPS. The blood and intestinal samples were collected at 4 h post injection. Supplementation with MCT improved intestinal morphology, digestive and barrier function, indicated by increased jejunal villus height, increased jejunal and ileal disaccharidases (sucrase and maltase) activities, as well as enhanced protein expression of claudin-1. Furthermore, the protein expression of heat-shock protein 70 in jejunum and the concentration of TNF-α in plasma were reduced in the piglets fed diets supplemented with MCT. In addition, MCT down-regulated the mRNA expression of toll-like receptor 4 (TLR4) and nucleotide-binding oligomerisation domain proteins (NOD) signalling-related genes in jejunum and ileum. Finally, MCT inhibited jejunal and ileal enterocyte necroptosis indicated by suppressed mRNA expression of the receptor-interacting protein 3 and mixed-lineage kinase domain-like protein. These results indicate that MCT supplementation may be closely related to inhibition of TLR4, NOD and necroptosis signalling pathways and concomitant improvement of intestinal integrity under an inflammatory condition. Copyright © The Authors 2018.
语种：
英文
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Effects of Biotite V supplementation on growth performance and the immunological responses of weaned pigs after an Escherichia coli lipopolysaccharide challenge
作者：
Guo, Ling;Liu, Yulan* ;Han, Jie;Zhu, Huiling;Wang, Xiuying
期刊：
Livestock Science ,2017年195:112-117 ISSN：1871-1413
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Han, Jie; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Biotite V;Immunological challenge;Lipopolysaccharide;Weaned pigs
摘要：
This study evaluated the effect of an aluminosilicate mineral additive-Biotite V (BV) on growth performance, the immunological and adrenal responses in weaned pigs after Escherichia coli lipopolysaccharide (LPS) challenge. Thirty-two crossbred weaned pigs were used in this 2x2 factorial experiment, with dietary treatment (basal diet with or without 0.45% BV supplementation) and LPS challenge (challenged or not challenged) as two main factors. On day 14 and 21, pigs were injected intraperitoneally with either 100 mu g/kg body weight of LPS or an equivalent amount of sterile saline. Blood samples were collected 3 h after the first challenge followed by total and differential leukocyte counts and analysis of plasma tumor necrosis factor-alpha (TNF-alpha), prostaglandin E-2 (PGE(2)), cortisol, insulin, and biochemical parameters. Body weight and feed intake were measured weekly throughout the 4-week experiment. The results showed that both LPS challenges reduced average daily gain (ADG) (P < 0.05) and increased feed/gain (P < 0.001), and BV decreased feed/gain (P < 0.05) of immunology challenged pigs. The LPS challengexdiet interactions were observed for ADG (P < 0.05) and feed/gain (P < 0.05) during both challenge. LPS challenge reduced the number of white blood cells, lymphocytes, monocytes, and neutrophils (P < 0.05). LPS challengexdiet interaction was observed for plasma TNF-alpha (P < 0.05) concentration, showing decreased plasma TNF-alpha response to LPS challenged in pigs receiving BV. But no LPS challenge x diet interaction was observed for plasma cortisol or PGE(2). There was a diet effect for plasma insulin (P < 0.05) and glucose (P < 0.05), showing increased plasma insulin and decreased glucose with BV supplementation, but no LPS challengexdiet interaction was observed. We conclude that BV improved the weight gain and feed efficiency of weaned pigs during an immunological challenge by suppressing the proinflammatory cytokine release.
语种：
英文
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Asparagine preserves intestinal barrier function from LPS-induced injury and regulates CRF/CRFR signaling pathway:
作者：
Zhu, Huiling;Pi, Dingan;Leng, Weibo;Wang, Xiuying;Hu, Chien-An Andy;...
期刊：
INNATE IMMUNITY ,2017年23(6):546-556 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Hu, Chien-An Andy; Qin, Qin; Liu, Yulan; Leng, Weibo; Xiong, Jianglin; Hou, Yongqing; Wang, Chunwei; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Liu, Yulan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Collaborat Innovat Ctr Anim Nutr & Feed Safety, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Collaborat Innovat Ctr Anim Nutr & Feed Safety, Wuhan 430023, Hubei, Peoples R China.
关键词：
*Asparagine;*CRF/CRFR1 signaling pathway;*intestinal barrier function;*lipopolysaccharide;*weaned pigs
摘要：
Stress causes intestinal inflammation and barrier dysfunction. Corticotrophin-releasing factor (CRF)/CRF receptor (CRFR) signaling pathway has been shown to be important for stress-induced intestinal mucosal alteration. L-Asparagine (ASN) is a powerful stimulator of ornithine decarboxylase and cell proliferation in a variety of cell types, including colonic cells. In the present study, we investigated whether dietary ASN supplementation could alleviate the damage of intestinal barrier function caused by LPS through modulation of CRF/CRFR signaling pathway. Twenty-four weaned pigs were randomly divided into one of four treatments: (1) non-challenged control; (2) Escherichia coli LPS challenged control; (3) LPS + 0.5% ASN; (4) LPS + 1.0% ASN. LPS stress induced villous atrophy, intestinal morphology disruption and decreased claudin-1 expression. ASN supplementation increased intestinal claudin-1 protein expression and alleviated villous atrophy and intestinal morphology impairment caused by LPS stress. In addition, ASN supplementation increased the number of intestinal intraepithelial lymphocytes and reversed the elevations of intestinal mast cell number and neutrophil number induced by LPS stress. Moreover, ASN decreased the mRNA expression of intestinal CRF, glucocorticoid receptors and tryptase. These results indicate that ASN attenuates intestinal barrier dysfunction induced by LPS stress, and regulates CRF/CRFR1 signaling pathway and mast cell activation. © The Author(s) 2017.
语种：
英文
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Aspartate inhibits LPS-induced MAFbx and MuRF1 expression in skeletal muscle in weaned pigs by regulating Akt, AMPKα and FOXO1:
作者：
Liu, Yulan* ;Wang, Xiuying;Leng, Weibo;Pi, Dingan;Tu, Zhixiao;...
期刊：
INNATE IMMUNITY ,2017年23(1):34-43 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Hu, Chien-An Andy; Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Tu, Zhixiao; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Liu, Yulan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Changqing Garden, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Changqing Garden, Wuhan 430023, Hubei, Peoples R China.
关键词：
Aspartate;lipopolysaccharide;MAFbx;MuRF1;muscle atrophy;piglets
摘要：
Infection and inflammation can result in the rapid loss of muscle mass and myofibrillar proteins (muscle atrophy). In addition, aspartate (Asp) is necessary for protein synthesis in mammalian cells. We hypothesized that Asp could attenuate LPS-induced muscle atrophy in a piglet model. Twenty-four weaning piglets were allotted to four treatments, including non-challenged control, LPS challenged control, LPS+0.5% Asp and LPS+1.0% Asp. On d 21, the piglets were injected with i.p. injection of LPS (100 ug/kg BM) or saline. At 4 h post-injection, blood, gastrocnemius and longissimus dorsi muscles samples were collected for biochemical and molecular analyses. Asp decreased the concentrations of cortisol and glucagon in plasma. In addition, Asp increased protein and RNA contents in muscles, and decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1). Moreover, Asp decreased phosphorylation of AMPKα but increased phosphorylation of Akt and Forkhead Box O (FOXO) 1 in the muscles. Our results indicate that Asp suppresses LPS-induced MAFbx and MuRF1 expression via activation of Akt signaling, and inhibition of AMPKα and FOXO1 signaling. © SAGE Publications.
语种：
英文
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Glutamate alleviates muscle protein loss by modulating TLR4, NODs, Akt/FOXO and mTOR signaling pathways in LPS-challenged piglets
作者：
Kang, Ping;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;Hou, Yongqing;...
期刊：
PLOS ONE ,2017年12(8):e0182246- ISSN：1932-6203
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
Gastrocnemius muscles;Muscle proteins;Swine;Diet;Toll-like receptors;Glutamate;Immune receptor signaling;Skeletal muscles
摘要：
The experiment was conducted to study the effect of the glutamate (Glu) on muscle protein loss through toll-like receptor 4 (TLR4), nucleotide-binding oligomerization domain proteins (NODs), Akt/Forkhead Box O (Akt/FOXO) and mammalian target of rapamycin (mTOR) signaling pathways in LPS-challenged piglets. Twenty-four weaned piglets were assigned into four treatments: (1) Control; (2) LPS+0% Glu; (3) LPS+1.0% Glu; (4) LPS+2.0% Glu. The experiment was lasted for 28 days. On d 28, the piglets in the LPS challenged groups were injected with LPS on 100 mu g/kg body weight (BW), and the piglets in the control group were injected with the same volume of 0.9% NaCl solution. After 4 h LPS or saline injection, the piglets were slaughtered and the muscle samples were collected. Glu supplementation increased the protein/DNA ratio in gastrocnemius muscle, and the protein content in longissimus dorsi (LD) muscle after LPS challenge (P < 0.05). In addition, Glu supplementation decreased TLR4, IL-1 receptor-associated kinase (IRAK) 1, receptor-interacting serine/threonine- protein kinase (RIPK) 2, and nuclear factor-kappa B (NF-kappa B) mRNA expression in gastrocnemius muscle (P < 0.05), MyD88 mRNA expression in LD muscle, and FOXO1 mRNA expression in LD muscle (P < 0.05). Moreover, Glu supplementation increased p-Akt/t-Akt ratio (P < 0.05) in gastrocnemius muscle, and p-4EBP1/t-4EBP1 ratio in both gastrocnemius and LD muscles (P < 0.05). Glu supplementation in the piglets' diets might be an effective strategy to alleviate LPS-induced muscle protein loss, which might be due to suppressing the mRNA expression of TLR4 and NODs signaling-related genes, and modulating Akt/ FOXO and mTOR signaling pathways.
语种：
英文
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Aspartate attenuates intestinal injury and inhibits TLR4 and NODs/NF-κB and p38 signaling in weaned pigs after LPS challenge
作者：
Wang, Haibo;Liu, Yulan* ;Shi, Haifeng;Wang, Xiuying;Zhu, Huiling;...
期刊：
European Journal of Nutrition ,2017年56(4):1433-1443 ISSN：1436-6207
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Wang, Haibo; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
Aspartate;Weaned piglets;Intestine injury;Lipopolysaccharide;Inflammatory response
摘要：
Purpose: This study was conducted to investigate whether aspartate (Asp) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by modulating intestine inflammatory response. Methods: Twenty-four weaned piglets were divided into four treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 0.5 % Asp; and (4) LPS + 1.0 % Asp. After feeding with control, 0.5 or 1.0 % Asp-supplemented diets for 21 days, pigs were injected intraperitoneally with saline or LPS. At 4 h postinjection, blood and intestine samples were obtained. Results: Asp supplementation to LPS-challenged pigs improved intestinal morphology, indicated by higher jejunal and ileal villus height/crypt depth ratio and lower ileal crypt depth linearly or quadratically. Asp also improved intestinal barrier function, indicated by increased jejunal and ileal diamine oxidase activities as well as enhanced protein expression of jejunal claudin-1 linearly or quadratically. In addition, Asp decreased plasma, jejunal and ileal tumor necrosis factor-α concentration and ileal caspase-3 protein expression linearly and quadratically. Moreover, Asp down-regulated the mRNA expression of toll-like receptor 4 (TLR4) and nucleotide-binding oligomerization domain protein (NOD) signaling-related genes, nuclear factor-κB (NF-κB) p65 and p38, decreased phosphorylation of jejunal p38, and increased phosphorylation of ileal extracellular signal-related kinase 1/2 linearly or quadratically. Finally, Asp increased mRNA expressions of TLR4 and NOD signaling negative regulators including radioprotective 105, suppressor of cytokine signaling 1, toll-interacting protein, Erbb2 interacting protein and centaurin β1 linearly or quadratically. Conclusions: These results indicate that Asp supplementation is associated with inhibition of TLR4 and NODs/NF-κB and p38 signaling pathways and concomitant improvement of intestinal integrity under an inflammatory condition. © 2016, Springer-Verlag Berlin Heidelberg.
语种：
英文
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TLR4信号通路负调控因子在断奶仔猪不同组织中的mRNA表达
作者：
王树辉;刘玉兰;王海波;陈少魁;朱惠玲;...
期刊：
中国畜牧杂志 ,2016年52(3):46-49 ISSN：0258-7033
作者机构：
武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;[祝爱侠; 王海波; 赵胜军; 王树辉; 任莹; 陈少魁; 朱惠玲; 刘玉兰] 武汉轻工大学
会议名称：
中国畜牧兽医学会动物营养分会第七届中国猪营养学术研讨会
会议时间：
2015-10-01
会议地点：
重庆
会议论文集名称：
中国畜牧兽医学会动物营养分会第七届中国猪营养学术研讨会论文集
关键词：
TLR4信号通路;断奶仔猪;负调控因子
摘要：
本试验旨在研究Toll样受体4(Toll-like receptor-4,TLR4)信号通路负调控因子在断奶仔猪不同组织的分布情况。试验选取12头杜×长×大断奶仔猪,屠宰,取脾脏、胸腺、下丘脑、垂体、肾上腺、空肠、回肠、肝脏、背最长肌和腓肠肌等组织。应用实时荧光定量PCR技术测定TLR4信号通路负调控因子在仔猪不同组织中的mRNA表达水平,包括单免疫球蛋白IL-1相关蛋白(Single immunoglobulin IL-1 R-related molecule,SIGIRR)、细胞因子信号传导抑制因子1(Suppressor ofcytokine signaling 1,SOCS1)、防辐射105 (Radioprotective 105,RP105)和Toll反应蛋白(Toll-interacting protein,Tollip)。结果表明：TLR4信号通路负调控因子在所测定的10个组织中均有一定程度的表达,但是组织分布规律存在着一定的差异。总体看来,主要在肝脏、回肠和脾脏中的表达量较高,在胸腺、垂体和空肠中的表达量居中,在其它组织中的表达量较低。综上所述,TLR4信号通路负调控因子在不同组织中表达差异较大,可能是负调控因子在TLR4信号传导通路中结合的传导因子与结合位点的不同,以及机体对负调控因子的适应机制不同,造成不同组织中负调控因子的mRNA表达量不同。
语种：
中文
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脂多糖刺激对仔猪肠道、肝脏和肌肉组织NODs信号通路关键基因及其负调控因子mRNA表达的影响
作者：
涂治骁;刘玉兰;吴欢听;王秀英;朱惠玲;...
期刊：
中国畜牧杂志 ,2016年52(11):35-38,54 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[康萍; 吴欢听; 涂治骁; 王秀英; 张晶; 朱惠玲; 刘玉兰] 武汉轻工大学
关键词：
仔猪;脂多糖;NODs信号通路
摘要：
本试验旨在研究脂多糖（LPS）刺激对仔猪核苷酸结合寡聚化结构域蛋白（NODs）信号通路关键基因及其负调控因子m RNA表达的影响。选取12头杜×长×大断奶仔猪,分成2个处理组,每个处理组6个重复。试验组注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后,屠宰仔猪,取空肠、回肠、肝脏、背最长肌和腓肠肌,应用实时荧光定量PCR技术测定NODs信号通路关键基因及其负调控因子m RNA表达水平,包括NOD1、NOD2、受体相互作用蛋白激酶2（RIPK2）、核因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）、矢车菊苷β1（ACAP1）和Erbb2相互作用蛋白（ERBB2IP）m RNA表达水平。结果表明:与对照组相比,LPS刺激显著提高了肝脏NOD1、NOD2、RIPK2、NF-κB和TNF-α,背最长肌NOD2、RIPK2和TNF-α,腓肠肌NOD2、RIPK2、NF-κB和TNF-α,空肠RIPK2和TNF-α的m RNA表达量（P<0.05）,LPS刺激有提高回肠RIPK2和NF-κB m RNA表达量的趋势（P<0.10）;同时,LPS刺激显著降低了空肠ACAP1和ERBB2IP,回肠和肝脏ACAP1的m RNA的表达量（P<0.05）。这表明,LPS激活仔猪肠道、肝脏和肌肉组织中NODs信号通路关键基因的表达,而抑制其负调控因子ACAP1和ERBB2IP的表达。
语种：
中文
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亚麻油对脂多糖诱导仔猪肠道损伤的调控作用
作者：
王树辉;涂治骁;王海波;陈少魁;朱惠玲;...
作者机构：
[王树辉; 涂治骁; 王海波; 陈少魁; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
仔猪;脂多糖;亚麻油;肠道损伤
摘要：
本试验研究了饲粮添加5%亚麻油对脂多糖诱导仔猪肠道损伤的调控作用,并从组织脂肪酸组成、形态学结构、组织功能指标以及抗炎的核苷酸结合寡聚域受体(NOD)和Toll样受体4(TLR4)信号通路基因的表达等方面探讨其作用机理。选择24头体重为(9.15±0.8)kg的杜(长(大三元杂交断奶仔猪,随机分为4个处理。本试验采用2×2因子设计,两个因子分别为饲粮处理和应激处理,得到以下四个分组:1)玉米油+生
语种：
中文
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甘氨酸对脂多糖刺激仔猪肝脏能量代谢及相关基因表达的调控作用
作者：
张琳;王秀英;吴欢听;王海波;陈少魁;...
期刊：
动物营养学报 ,2016年28(12):4006-4013 ISSN：1006-267X
作者机构：
[张琳; 王秀英; 吴欢听; 王海波; 陈少魁; 朱惠玲; 刘玉兰] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
仔猪;脂多糖;甘氨酸;肝脏;能量代谢
摘要：
本试验旨在研究甘氨酸(Gly)对脂多糖(LPS)刺激断奶仔猪肝脏能量代谢、能量代谢关键酶和相关调节因子mRNA表达的调控作用。选取24头杜×长×大仔猪,分成4个处理,每个处理6个重复。4个处理组分别为:1)对照组(基础饲粮);2)LPS组(LPS+基础饲粮);3)1.0%Gly组(LPS+基础饲粮+1.0%Gly);4)2.0%Gly组(LPS+基础饲粮+2.0%Gly)。试验第28天,2、3、4
语种：
中文
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亚麻籽油对脂多糖刺激仔猪肝脏Toll样受体4和核苷酸结合寡聚化结构域信号通路关键基因表达的影响
作者：
陈少魁;刘玉兰;王海波;王秀英;朱惠玲;...
期刊：
动物营养学报 ,2016年28(3):891-898 ISSN：1006-267X
作者机构：
[陈少魁; 刘玉兰; 王海波; 王秀英; 朱惠玲; 张晶; 王树辉; 涂治骁] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023
关键词：
仔猪;亚麻籽油;脂多糖;肝脏
摘要：
本试验旨在研究亚麻籽油对脂多糖(LPS)刺激仔猪肝脏Toll样受体4(TLR4)和核苷酸结合寡聚化结构域(NOD)信号通路关键基因表达的影响。选取24头断奶仔猪,按体重相近原则随机分为4个组,分别为对照组、LPS组、2.5%亚麻籽油组(2.5%亚麻籽油+LPS)、5.0%亚麻籽油组(5.0%亚麻籽油+LPS),每组6个重复,每个重复1头猪,试验期21 d。试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后屠宰仔猪,取肝脏,测定TLR4和NOD信号通路关键基因及相关炎性介质的mRNA表达水平。结果表明:1)LPS刺激显著提高了肝脏肿瘤坏死因子-α(TNF-α)、环氧酶2(COX2)、热休克蛋白70(HSP70)的mRNA相对表达量(P<0.05),2.5%亚麻籽油可显著降低COX2、TNF-α的mRNA相对表达量(P<0.05),5.0%亚麻籽油可显著降低TNF-α的mRNA相对表达量(P<0.05)。2)LPS刺激显著提高了肝脏TLR4、髓样分化因子88(MyD88)、白细胞介素-1受体相关激酶1(IRAK1)、NOD1、NOD2、受体互作蛋白2(RIPK2)、核因子-κB(NF-κB)的mRNA相对表达量(P<0.05);2.5%亚麻籽油可显著降低NOD1、NOD2的mRNA相对表达量(P<0.05),有降低RIPK2 mRNA相对表达量的趋势(0.05≤P<0.10);5.0%亚麻籽油可显著降低NOD2的mRNA相对表达量(P<0.05)。这表明LPS刺激导致仔猪发生炎症反应,亚麻籽油可能通过抑制NOD信号通路进而缓解肝脏炎症反应。
语种：
中文
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中链脂肪酸对脂多糖诱导的仔猪肝脏损伤的调控作用
作者：
涂治骁;陈少魁;王海波;王树辉;朱惠玲;...
作者机构：
[涂治骁; 陈少魁; 王海波; 王树辉; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
仔猪;中链脂肪酸;肝脏损伤
摘要：
本文通过测定肝脏脂肪酸组成、形态结构与功能、核苷酸结合寡聚域受体(NOD)和Toll样受体4(TLR4)信号通路及其下游相关基因的表达,探讨了中链脂肪酸对大肠杆菌脂多糖(LPS)诱导的断奶仔猪肝脏损伤的调控及其机制。试验共选用24头杜×长×大断奶仔猪[体重(9.0±0.7)kg],分为4个组。试验采用2×2因子设计,两个主因子分别:1)饲粮处理(玉米油或中链脂肪酸);2)免疫应激处理(0.9%Na
语种：
中文
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谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响
作者：
秦琴;王秀英;吴欢听;朱惠玲;刘玉兰
作者机构：
[秦琴; 王秀英; 吴欢听; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
谷氨酸;脂多糖;断奶仔猪;肠道;能量代谢
摘要：
本试验旨在研究谷氨酸(Glu)对脂多糖(LPS)刺激断奶仔猪肠道能量代谢的影响。选择24头断奶仔猪,分为4个处理组:1)对照组;2)LPS+0%Glu组;3)LPS+1.0%Glu组;4)LPS+2.0%Glu组。在正式试验第28天,2、3、4组猪注射100μg/kg BW LPS,对照组注射等量的生理盐水,4 h后屠宰,取肠道样品待测。结果表明:1)LPS刺激导致空肠三磷酸腺苷(ATP)、腺苷酸
语种：
中文
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Asparagine improves intestinal integrity, inhibits TLR4 and NOD signaling, and differently regulates p38 and ERK1/2 signaling in weanling piglets after LPS challenge:
作者：
Chen, Shaokui;Liu, Yulan* ;Wang, Xiuying;Wang, Haibo;Li, Shuang;...
期刊：
INNATE IMMUNITY ,2016年22(8):577-587 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Jing; Hu, Chien-An Andy; Liu, Yulan; Chen, Shaokui; Li, Shuang; Shi, Haifeng; Wang, Haibo; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC USA.;[Liu, Yulan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Asparagine;weanling piglets;intestine;lipopolysaccharide;inflammatory response
摘要：
Asparagine (Asn), an activator of ornithine decarboxylase (ODC), stimulates cell proliferation in intestinal epithelial cells. We hypothesized that Asn can mitigate LPS-induced injury of intestinal structure and barrier function by regulating inflammatory signaling pathways. We executed the following experiment using weanling pigs for each of the groups: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 0.5% Asn; (4) LPS + 1.0% Asn. After 21-d feeding, pigs received an i.p. injection of either saline or LPS. Four h after injection, the mid-jejunum and mid-ileum samples were collected. We found that Asn restored ODC expression that was decreased by LPS treatment. Asn also restored intestinal morphology and barrier function that were impaired by LPS treatment. In addition, Asn down-regulated intestinal caspase-3 protein expression and TNF-α concentration, and decreased the mRNA expression of intestinal TLR4, TLR4 downstream signals (myeloid differentiation factor 88, IL-1 receptor-associated kinase 1 and TNF-α receptor-associated factor 6 and NOD1, NOD2 and their adaptor molecule (receptor-interacting serine/threonine-protein kinase 2). Moreover, Asn decreased p38 phosphorylation but increased ERK1/2 phosphorylation. Our results suggest that Asn improves intestinal integrity during an inflammatory insult, which appears to be related to the decrease of intestinal pro-inflammatory cytokine (via TLR4, NODs and p38) and of enterocyte apoptosis (via p38 and ERK1/2). © SAGE Publications.
语种：
英文
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Dietary Supplementation with α-Ketoglutarate Activates mTOR Signaling and Enhances Energy Status in Skeletal Muscle of Lipopolysaccharide-Challenged Piglets
作者：
Wang, Lei;Yi, Dan;Hou, Yongqing* ;Ding, Binying;Li, Kang;...
期刊：
The Journal of Nutrition ,2016年146(8):1514-1520 ISSN：0022-3166
通讯作者：
Hou, Yongqing;Wu, Guoyao
作者机构：
[Li, Kang; Liu, Yulan; Li, Baocheng; Hou, Yongqing; Wu, GY; Wang, Lei; Wu, Guoyao; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, YQ; Wu, GY] W;[Wu, Guoyao] T;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Peoples R China.;Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
关键词：
*cell signaling;*gastrocnemius muscle;*inflammation;*lipopolysaccharide;*alpha-ketoglutarate
摘要：
Background: Skeletal muscle undergoes rapid loss in response to inflammation. a-Ketoglutarate (AKG) has been reported to enhance muscle growth in piglets, but the underlying mechanisms are largely unknown. Objectives: This study tested the hypothesis that dietary AKG supplementation activates mechanistic target of rapamycin (mTOR) signaling and improves skeletal muscle energy metabolism in lipopolysaccharide (LPS)-challenged piglets. Methods: Forty-eightmale piglets (Duroc × Landrace × Yorkshire)wereweaned at 21 d of age to a corn- and soybeanmeal- based diet. After a 3-d period of adaptation, piglets with a mean weight of 7.21 kg were randomly assigned to control, LPS (intraperitoneal administration of 80 μg LPS/kg body weight on days 10, 12, 14, and 16), or LPS plus 1% dietary AKG (LPS +AKG) groups. On day 16, blood sampleswere collected from8 piglets/group 3 h after LPS administration.On day 17, piglets were killed to obtain gastrocnemius muscle from 8 piglets/group for biochemical analysis. Results: Compared with the control group, LPS administration increased (P < 0.05) plasma concentrations of globulin (by 14%) and tumor necrosis factor α (by 59%) and the intramuscular ratio of AMP to ATP (by 93%) and abundance of phosphorylated acetyl- coenzyme A carboxylase (ACC) β protein (by 64%). Comparedwith the control group, LPS administration reduced (P < 0.05) weight gain (by 15%); plasma concentrations of glutamine (by 20%), glucose (by 23%), insulin, insulin-like growth factor I, and epidermal growth factor; intramuscular concentrations of glutamine (by 27%), ATP (by 12%), ADP (by 22%), and total adenine nucleotides; and intramuscular ratios of phosphorylated mTOR to total mTOR (by 38%) and of phosphorylated 70-kDa ribosomal protein S6 kinase (p70S6K) to total p70S6K (by 39%). These adverse effects of LPS were ameliorated (P < 0.05) by AKG supplementation. Conclusions: Dietary AKG supplementation activated mTOR signaling, inhibited ACC-β, and improved energy status in skeletal muscle of LPS-challenged piglets. These results provide a biochemical basis for the use of AKG to enhance piglet growth under inflammatory or practical postweaning conditions. © 2016 American Society for Nutrition.
语种：
英文
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谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响
作者：
秦琴;王秀英;吴欢听;朱惠玲;刘玉兰
期刊：
动物营养学报 ,2016年28(11):3618-3625 ISSN：1006-267X
作者机构：
[秦琴; 王秀英; 吴欢听; 朱惠玲; 刘玉兰] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023
关键词：
谷氨酸;脂多糖;断奶仔猪;肠道;能量代谢
摘要：
本试验旨在研究谷氨酸（ Glu）对脂多糖（ LPS）刺激断奶仔猪肠道能量代谢的影响。选择24头断奶仔猪分为4个组，分别为对照组、LPS组、LPS＋1．0％ Glu组和LPS＋2．0％ Glu组，每组6个重复，每个重复1头猪。于试验第28天，试验组猪注射100μg／kg BW LPS，对照组注射等量的生理盐水，4 h后屠宰，取肠道样品待测。结果表明：1）与对照组相比，LPS刺激导致断奶仔猪空肠三磷酸腺苷（ATP）、腺苷酸池（TAN）含量和能荷（EC）显著降低（P＜0．05），一磷酸腺苷（ AMP）／ATP值显著升高（ P＜0．05）；与LPS组相比，LPS＋2．0％ Glu组显著提高了空肠ATP、二磷酸腺苷（ ADP）和TAN含量（ P＜0．05）。2）与对照组相比，LPS刺激导致断奶仔猪回肠柠檬酸合成酶和α－酮戊二酸脱氢酶系活性极显著降低（ P＜0．01），空肠α－酮戊二酸脱氢酶系活性有降低趋势（ P＝0．092）；与LPS组相比，除LPS＋1．0％ Glu组回肠柠檬酸合成酶活性显著降低（ P＜0．05）外，Glu对空肠和回肠三羧酸循环关键酶活性无显著影响（ P＞0．05）。3）与对照组相比， LPS刺激导致空肠过氧化物酶体增殖物激活受体γ辅激活因子1α（ PGC1α）及回肠沉默信号调控因子1（Sirt1）和PGC1α的mRNA表达量极显著降低（P＜0．01）；与LPS组相比，LPS＋2．0％Glu组有提高空肠PGC1α（P＝0．067）和回肠Sirt1（P＝0．053）mRNA表达量的趋势，LPS＋1．0％Glu组有提高回肠Sirt1 mRNA表达量的趋势（ P＝0．070）。由此可见，Glu可以改善LPS刺激导致的肠道能量损耗状态。
语种：
中文
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Asparagine reduces the mRNA expression of muscle atrophy markers via regulating protein kinase B (Akt), AMP-activated protein kinase α, toll-like receptor 4 and nucleotide-binding oligomerisation domain protein signalling in weaning piglets after lipopolysaccharide challenge
作者：
Wang, Xiuying;Liu, Yulan* ;Wang, Shuhui;Pi, Dingan;Leng, Weibo;...
期刊：
British Journal of Nutrition ,2016年116(7):1188-1198 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Jing; Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Wang, Shuhui; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Asparagine;Lipopolysaccharides;Muscle atrophy;Pro-inflammatory cytokines
摘要：
Pro-inflammatory cytokines are critical in mechanisms of muscle atrophy. In addition, asparagine (Asn) is necessary for protein synthesis in mammalian cells. We hypothesised that Asn could attenuate lipopolysaccharide (LPS)-induced muscle atrophy in a piglet model. Piglets were allotted to four treatments (non-challenged control, LPS-challenged control, LPS+0·5 % Asn and LPS+1·0 % Asn). On day 21, the piglets were injected with LPS or saline. At 4 h post injection, piglet blood and muscle samples were collected. Asn increased protein and RNA content in muscles, and decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1). However, Asn had no effect on the protein abundance of MAFbx and MuRF1. In addition, Asn decreased muscle AMP-activated protein kinase (AMPK) α phosphorylation, but increased muscle protein kinase B (Akt) and Forkhead Box O (FOXO) 1 phosphorylation. Moreover, Asn decreased the concentrations of TNF-α, cortisol and glucagon in plasma, and TNF-α mRNA expression in muscles. Finally, Asn decreased mRNA abundance of muscle toll-like receptor (TLR) 4 and nucleotide-binding oligomerisation domain protein (NOD) signalling-related genes, and regulated their negative regulators. The beneficial effects of Asn on muscle atrophy may be associated with the following: (1) inhibiting muscle protein degradation via activating Akt and inactivating AMPKα and FOXO1; and (2) decreasing the expression of muscle pro-inflammatory cytokines via inhibiting TLR4 and NOD signalling pathways by modulation of their negative regulators. © The Authors 2016.
语种：
英文
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Fish oil enhances intestinal barrier function and inhibits corticotropin-releasing hormone/corticotropin-releasing hormone receptor 1 signalling pathway in weaned pigs after lipopolysaccharide challenge
作者：
Zhu, Huiling;Liu, Yulan* ;Chen, Shaokui;Wang, Xiuying;Pi, Dingan;...
期刊：
British Journal of Nutrition ,2016年115(11):1947-1957 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Jing; Liu, Yulan; Leng, Weibo; Chen, Shaokui; Kang, Ping; Chen, Feng; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Lipopolysaccharides;Fish oil;Intestinal barrier function;Weaned pigs
摘要：
Stress induces injury in intestinal barrier function in piglets. Long-chain n-3 PUFA have been shown to exhibit potential immunomodulatory and barrier protective effects in animal models and clinical trials. In addition, corticotropin-releasing hormone (CRH)/CRH receptor (CRHR) signalling pathways play an important role in stress-induced alterations of intestinal barrier function. We hypothesised that fish oil could affect intestinal barrier function and CRH/CRHR signalling pathways. In total, thirty-two weaned pigs were allocated to one of four treatments. The experiment consisted of a 2×2 factorial design, and the main factors included immunological challenge (saline or lipopolysaccharide (LPS)) and diet (5 % maize oil or 5 % fish oil). On d 19 of the trial, piglets were treated with saline or LPS. At 4 h after injection, all pigs were killed, and the mesenteric lymph nodes (MLN), liver, spleen and intestinal samples were collected. Fish oil decreased bacterial translocation incidence and the number of translocated micro-organisms in the MLN. Fish oil increased intestinal claudin-1 protein relative concentration and villus height, as well as improved the intestinal morphology. In addition, fish oil supplementation increased intestinal intraepithelial lymphocyte number and prevented elevations in intestinal mast cell and neutrophil numbers induced by LPS challenge. Moreover, fish oil tended to decrease the mRNA expression of intestinal CRHR1, CRH and glucocorticoid receptors. These results suggest that fish oil supplementation improves intestinal barrier function and inhibits CRH/CRHR1 signalling pathway and mast cell tissue density. © The Authors 2016.
语种：
英文
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鱼油提高LPS应激仔猪小肠屏障功能、抑制CRH/CRHR1信号通路
作者：
朱惠玲;刘玉兰;陈少魁;王秀英;皮定安;...
作者机构：
[朱惠玲; 刘玉兰; 陈少魁; 王秀英; 皮定安; 冷炜博; 陈逢; 张晶; 康萍] 武汉轻工大学动物营养与饲料科学湖北省重点实验室动物营养与饲料安全湖北省协同创新中心
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
会议论文集名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集
关键词：
脂多糖;鱼油;小肠屏障功能;断奶仔猪
摘要：
应激可引起仔猪小肠屏障功能障碍。大量研究表明,糖皮质激素释放激素(CRH)/糖皮质激素释放激素受体1(CRHR1)信号通路在应激诱导小肠屏障功能障碍中发挥重要作用。临床试验及动物模型研究表明,n-3多不饱和脂肪酸具有免疫调节及屏障保护功能。本试验用脂多糖(LPS)刺激建立免疫应激模型,研究鱼油对LPS应激仔猪小肠屏障功能及CRH/CRHR1信号通路的影响,旨在为缓解仔猪免疫应激提供理论依据。选用3
语种：
中文
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Glycine enhances muscle protein mass associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing TLR4 and NOD2 signaling in piglets challenged with LPS
作者：
Liu, Yulan* ;Wang, Xiuying;Wu, Huanting;Chen, Shaokui;Zhu, Huiling;...
期刊：
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY ,2016年311(2):R365-R373 ISSN：0363-6119
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Zhang, Guolong; Zhang, Jing; Hu, Chien-An Andy; Liu, Yulan; Chen, Shaokui; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
glycine;lipopolysaccharide;muscle;pro-inflammatory cytokines
摘要：
Pro-inflammatory cytokines play a critical role in the pathophysiology of muscle atrophy. We hypothesized that glycine exerted an anti-inflammatory effect and alleviated lipopolysaccharide (LPS)-induced muscle atrophy in piglets. Pigs were assigned to four treatments including the following: 1) nonchallenged control, 2) LPS-challenged control, 3) LPS+1.0% glycine, and 4) LPS+2.0% glycine. After receiving the control, 1.0 or 2.0% glycine-supplemented diets, piglets were treated with either saline or LPS. At 4 h after treatment with saline or LPS, blood and muscle samples were harvested. We found that 1.0 or 2.0% glycine increased protein/DNA ratio, protein content, and RNA/DNA ratio in gastrocnemius or longissimus dorsi (LD) muscles. Glycine also resulted in decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1) in gastrocnemius muscle. In addition, glycine restored the phosphorylation of Akt, mammalian target of rapamycin (mTOR), eukaryotic initiation factor 4E binding protein 1 (4E-BP1), and Forkhead Box O 1 (FOXO1) in gastrocnemius or LD muscles. Furthermore, glycine resulted in decreased plasma tumor necrosis factor-α (TNF-α) concentration and muscle TNF-α mRNA abundance. Moreover, glycine resulted in decreased mRNA expresson of Toll-like receptor 4 (TLR4), nucleotide-binding oligomerization domain protein 2 (NOD2), and their respective downstream molecules in gastrocnemius or LD muscles. These results indicate glycine enhances muscle protein mass under an inflammatory condition. The beneficial roles of glycine on the muscle are closely associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing the activation of TLR4 and/or NOD2 signaling pathways. © 2016 the American Physiological Society.
语种：
英文
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