乳制品中微生物标准物质研究现状与趋势
作者：
刘思渊;柏赵莹;王梓权;王蒙;陆琳;...
期刊：
计量学报 ,2023年44(3):457-463 ISSN：1000-1158
作者机构：
[刘思渊; 王成龙; 柏赵莹] 中国计量科学研究院,北京100029;[刘思渊; 张伟] 河北农业大学食品科技学院,河北保定071001;[刘思渊; 张伟] 河北农业大学河北省人畜共患病原微生物分析与防控重点实验室,河北保定071001;[柏赵莹] 武汉轻工大学生命科学与技术学院,湖北武汉430023;[王成龙] 湖南师范大学生命科学学院,湖南长沙410081
关键词：
计量学;乳制品质量;微生物检测;标准物质
摘要：
乳制品中微生物标准物质是发展微生物计量技术，建立微生物检测标准，完善乳及乳制品质量监管体系的重要工具，具有广阔的需求及应用前景。介绍了乳制品中微生物标准物质的主要特性，保持微生物活性的主要制备技术，以及特性量值、标称特性的测量技术；此外，对乳制品中微生物标准物质发展和应用现状进行了综述，并在分析该领域研究面临的主要问题的基础上，对标准物质需求与趋势进行了展望，旨在为后续微生物标准物质研究提供一定的参考与借鉴。
语种：
中文
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Faster Detection of Staphylococcus aureus in Milk and Milk Powder by Flow Cytometry
作者：
Liu, Siyuan;Wang, Bin;Sui, Zhiwei* ;Wang, Ziquan;Li, Longquan;...
期刊：
FOODBORNE PATHOGENS AND DISEASE ,2021年18(5):346-353 ISSN：1535-3141
通讯作者：
Sui, Zhiwei
作者机构：
[Liu, Siyuan; Li, Longquan; Sui, Zhiwei; Wang, Ziquan; Wang, Bin] Natl Inst Metrol, Ctr Adv Measurement Sci, 18 Bei San Huan Dong Lu, Beijing 100029, Peoples R China.;[Li, Longquan; Zhou, Guoping] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Peoples R China.;[Zhen, Xiaoxiao] Beijing Inst Metrol, Inst Chem Anal & Biomed, Beijing, Peoples R China.;[Zhao, Wei] Nantong Customs, Dept Supervis, Nantong, Peoples R China.
通讯机构：
[Sui, Zhiwei] N;Natl Inst Metrol, Ctr Adv Measurement Sci, 18 Bei San Huan Dong Lu, Beijing 100029, Peoples R China.
关键词：
Staphylococcus aureus;detection;flow cytometry;milk;milk powder
摘要：
A flow cytometry (FCM)-based method was developed for the faster detection of Staphylococcus aureus in milk and milk powder. Viable S. aureus cells were recognized by highly selective, fluorescently labeled antibodies and Propidium Iodide, and then analyzed by FCM. Using a 5-h pre-enrichment period, the method could detect low numbers of S. aureus cells in 6 h, with a limit of detection of 7.50 cells/mL in milk and 8.30 cells/g in milk powder. The established method was compared with the plate-based method using 75 ultra-high-temperature-treated milk samples, 25 pasteurized milk samples, 66 raw milk samples, and 123 milk powder samples. The two methods yielded similar results for the detection of the pathogen in all sample types. The FCM-based method allows effective and faster monitoring of S. aureus contamination and can be applied to the rapid detection of microorganisms in milk and dairy products. © 2021 Mary Ann Liebert, Inc., publishers.
语种：
英文
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Characterization of three autolysins with activity against cereulide-producing Bacillus isolates in food matrices
作者：
Geng, Peiling;Hu, Yimin;Zhou, Guoping;Yuan, Zhiming* ;Hu, Xiaomin*
期刊：
International Journal of Food Microbiology ,2017年241:291-297 ISSN：0168-1605
通讯作者：
Yuan, Zhiming;Hu, Xiaomin
作者机构：
[Geng, Peiling; Yuan, Zhiming; Hu, Xiaomin; Hu, Yimin] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430070, Peoples R China.;[Geng, Peiling] Univ Chinese Acad Sci, Beijing 100039, Peoples R China.;[Zhou, Guoping] Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Yuan, ZM; Hu, Xiaomin] Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China.
通讯机构：
[Yuan, ZM; Hu, XM] C;Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China.
关键词：
autolysin;cereulide;bacterial protein;bacterial toxin;cereulide;depsipeptide;n acetylmuramoylalanine amidase;alkalophile;Article;assay;Bacillus cereus;bacterial strain;bactericidal activity;bacterium isolate;chemical analysis;controlled study;food;milk;nonhuman;pH;physical phenomena;rice;spore germination;thermal analysis;analysis;Bacillus cereus;diarrhea;drug effects;enzymology;food contamination;food control;genetics;human;isolation and purification;metabolism;microbiology;Bacillus cereus;Bacterial Proteins;Bacterial Toxins;Depsipeptides;Diarrhea;Food Contamination;Food Microbiology;Humans;N-Acetylmuramoyl-L-alanine Amidase
摘要：
Bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases. It is therefore necessary to develop an effective strategy to prevent the propagation of B. cereus in the food supply. In this study, three autolysins from the cereulide-producing B. cereus group isolates, LysIS075, LysF8819.1 and LysCER057, were identified and characterized. The results showed that the three autolysins were highly lytic and bactericidal to the tested cereulide-producing B. cereus group strains and cross-lytic against other tested B. cereus group strains, and they could inhibit the spore germination and propagation of their tested derived emetic strains. Physical and chemical characterization showed that all the three autolysins were alkalophilic with the optimal activity at pH9.0 or 9.5 with one exception of LysF8819.1 also having significant lytic activity at pH5.0, and they all had relative strong lytic activity at 37–50°C during the 30minute assay. However, LysCER057 showed relative susceptibility to thermo-condition. Remarkably, the separate or cock-tail addition of the three autolysins in food matrices (milk and rice porridge) showed effective bactericidal activity within the tested 2h. All the results revealed that the three autolysins might be potential candidates to control emetic B. cereus strains in different applications. © 2016
语种：
英文
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Genetic and genomic diversity of NheABC locus from Bacillus strains
作者：
Cai, Yan;Huang, Tingxuan;Xu, Yuekang;Zhou, Guoping;Zou, Ping;...
期刊：
Archives of Microbiology ,2017年199(5):775-785 ISSN：0302-8933
通讯作者：
Liu, Xiaojin
作者机构：
[Zeng, Guifeng; Cai, Yan; Zou, Ping] Changzhou Univ, Sch Food Sci & Technol, 1 Gehu Middle Rd, Changzhou 213164, Jiangsu, Peoples R China.;[Liu, Xiaojin; Xu, Yuekang] Anhui Normal Univ, Coll Life Sci, 1 Beijing East Rd, Wuhu 241002, Anhui, Peoples R China.;[Huang, Tingxuan; Zhou, Guoping] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Xiaojin] A;Anhui Normal Univ, Coll Life Sci, 1 Beijing East Rd, Wuhu 241002, Anhui, Peoples R China.
关键词：
Bacillus;NheABC;Genetic diversity;MLST
摘要：
Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurrence of NHE among them, and other members of Bacillus is unclear. Consequently, we aimed to determine the distribution and evolution of NHE within Bacillus by confirming the presence of the nheA, B and C sequences and variation within them using published data, and to analyze the genomic and genetic diversity. The phylogenetic tree of NHE proteins (NheA, NheB and NheC) from 81 different B. cereus s.l. strains was constructed. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis. Analysis of the genomic diversity of the nheA, B and C loci revealed that their upstream regions were more conserved than the downstream sequences. Multilocus sequence typing schemes (MLST) based on seven concatenated housekeeping genes and nheA, B and C genes of the 75 strains were developed. The neighbor joining phylogenetic tree based on seven housekeeping genes together with nheA, B and C genes was similiar to published Bacillus phylogenetic trees. And on the genetic determinants of the NHE toxin did not bring any obvious link between the nheABC genes sequence of a strain and its virulence in the diarrhoeal pathogenesis.The results indicate that nheA, B and C genes do not affect the diversity of housekeeping genes, and this specific NHE protein does not participate in the diarrheic syndrome.
语种：
英文
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经辐照杀菌的无菌袋中耐辐照微生物分离与鉴定
作者：
纪明淮;高雯;周帼萍
期刊：
食品科学 ,2016年37(22):205-209 ISSN：1002-6630
通讯作者：
Zhou, Guoping(wjczgp@163.com)
作者机构：
[周帼萍; 高雯; 纪明淮] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
关键词：
耐辐照;辐照杀菌的无菌袋;不动杆菌属;考克斯菌属;微球菌属;微杆菌属;芽孢杆菌属;栖水菌属;表皮葡萄球菌
摘要：
对某番茄酱生产企业提供的辐照杀菌(辐照剂量≥15 kGy)的5 L无菌袋进行菌落计数、分离和鉴定。3 L无菌水反复漂洗,滤膜过滤后进行平板菌落计数,分离纯化后采用16S rDNA和dnaJ序列分析分别鉴定到属和种。结果表明,5批次的样品中均有细菌检出,数量在5~132 CFU/袋,9个典型菌落经过16S rDNA序列分析后,分别属6个属,其中栖水菌属(Enhydrobacter)、不动杆菌属(Acinetobacter)和考克斯菌属(Kocuria)各1株,微球菌属(Micrococcus)、微杆菌属(Microbacterium)和芽孢杆菌属(Bacillus)各2株;dnaJ序列分析则将一株葡萄球菌C8-3鉴定到种-表皮葡萄球菌(Staphylococcus epidermidis)。辐照杀菌的无菌袋虽然微生物数量不高,但是依然残留了一些耐辐照的细菌,它们或多或少会给内装食品带来一定的食品安全隐患或影响食品保质期。
语种：
中文
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PET瓶装无气天然矿泉水中污染菌及其系统进化分析
作者：
高雯;胡晓敏;周帼萍
期刊：
食品科学 ,2016年37(8):196-200 ISSN：1002-6630
通讯作者：
Zhou, Guoping(wjczgp@163.com)
作者机构：
[周帼萍; 高雯] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[胡晓敏] Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
关键词：
PET瓶装无气天然矿泉水;异味;紫外杀菌;两级串联0.2 μm过滤器
摘要：
目的:了解某一批次聚对苯二甲酸乙二醇酯(polyethylene terephthalate,PET)瓶装无气天然矿泉水产生异味的缘由。方法:首先采用膜过滤结合平板菌落计数法分离和计数污染菌,16S rRNA序列分析鉴定其为假单胞菌属(Pseudomonas spp.),再采用假单胞菌属特异性rpoB引物进行梯度聚合酶链式反应优化扩增条件,产物进行测序和序列分析将污染菌鉴定到种。结果:这批产品的主要污染菌是一种2009年从南极分离鉴定的假单胞菌属新种P. extremaustralis。反接实验证实该菌能在天然矿泉水中生长繁殖,并产生刺激性异味。结论:P. extremaustralis部分细菌能够透过两级串联0.2 μm过滤器,耐受中、低压紫外杀菌工艺,耐低温和低氧压,能在无任何添加的PET瓶装无气天然矿泉水中生长并产生异味。
语种：
中文
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浓缩果汁中耐热霉菌的分析及鉴定
作者：
童迅;高雯;黄庭轩;周帼萍
期刊：
食品科学 ,2016年37(20):198-202 ISSN：1002-6630
通讯作者：
Zhou, Guoping(wjczgp@163.com)
作者机构：
[周帼萍; 高雯; 童迅; 黄庭轩] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
关键词：
浓缩果汁;耐热霉菌;检测与鉴定;正青霉;篮状菌
摘要：
目的:检测15个批次浓缩果汁中的耐热霉菌并对其进行鉴定。方法:80 ℃、30 min热处理后涂布平板菌落计数法分离和计数浓缩果汁中的耐热霉菌,分离纯化后观察其显微形态和菌落形态,再采用28S rDNA序列分析鉴定霉菌的种属。结果:这15批次浓缩果汁中有8个样品检出耐热霉菌,检出率达53.3%;检出的耐热霉菌以正青霉(Eupenicillium)和篮状菌(Talaromyces)为主,还有少量白耙齿菌、栓菌和座囊菌属。结论:进口或国产浓缩果汁都存在耐热霉菌污染,其中尤其值得注意的是检出了既耐热又能产生真菌毒素的正青霉和篮状菌。
语种：
中文
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基于能量法的非均质地基中单桩受扭弹塑性分析
作者：
邹新军;王英宇;赵明华;徐洞斌
期刊：
水文地质工程地质 ,2016年37(2):54-61 ISSN：1000-3665
作者机构：
湖南大学土木工程学院,湖南长沙,410082;湖南大学土木工程学院,湖南长沙410082;建发房地产集团有限公司,福建厦门 361008;[邹新军; 王英宇; 赵明华; 徐洞斌] 湖南大学
关键词：
单桩基础;非均质地基;扭矩;能量原理;有限差分法
摘要：
为探讨非均质地基中单桩受扭的非线性特性,假定桩侧地表处地基土剪切模量为非零值（已有方法多假定为零）且随深度呈幂函数分布,引入虚土桩法建立出桩土体系的总能量方程后,基于最小势能原理由变分法分别导得桩身及土体的受扭控制方程,进而采用有限差分法获得桩身扭转角的差分方程式。然后,将桩周土体视为理想弹塑性材料,给出了塑性状态下的单桩受扭分析方法,并基于MATLAB编制了计算程序,再将计算结果与已有方法及实验结果进行了对比验证。最后,通过参数分析获得了桩体参数G_p和r_0以及土体参数α_g和n的取值对桩身扭转响应的影响规律：随桩体剪切模量G_p的增大,桩顶扭转角减小;桩径r_0增大一倍时,相同扭转角下桩顶能承受的扭矩增大5~7倍;α_g≤0.1L时,扭矩传递先慢后快,反之则表现为先快后慢;n≤1时,扭矩传递先快后慢,反之则表现为先慢后快,且桩身扭转响应主要集中于桩身上部。
语种：
中文
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山西老陈醋样品的污染微生物分析
作者：
李盈颖;高雯;周帼萍
期刊：
食品科学 ,2016年37(12):226-231 ISSN：1002-6630
通讯作者：
Zhou, Guoping(wjczgp@163.com)
作者机构：
[周帼萍; 高雯; 李盈颖] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
关键词：
山西老陈醋;头状葡萄球菌头状亚种;产气;解淀粉芽孢杆菌
摘要：
为了解某批老陈醋产气变质的原因,采用橙血清和乳酸菌培养基分离和计数污染菌,显微形态观察结合16S rRNA序列分析鉴定其主要污染菌为葡萄球菌属(Staphylococcus sp.),再采用葡萄球菌属特异性dnaJ引物进行扩增,测序和序列分析将污染菌鉴定到头状葡萄球菌头状亚种(Staphylococcus capitis subsp. capitis);当使用醋酸菌培养基对老陈醋中沉淀进行分离时又发现少量芽孢杆菌,经芽孢杆菌属特异性rpoB引物鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。因此,导致该批次老陈醋产气变质的细菌主要是头状葡萄球菌头状亚种,少量解淀粉芽孢杆菌也存活于老陈醋中,但基本处于休眠态。电子舌检测显示:它们的存在和繁殖不仅导致产气,而且使醋的各种滋味变得平淡。
语种：
中文
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1型鸭甲型肝炎病毒3D基因的原核表达与多抗制备
作者：
王安平;朱善元;吴双
期刊：
安徽农业大学学报 ,2015年42(4):536-539 ISSN：1672-352X
作者机构：
[王安平; 朱善元; 吴双] 江苏农牧科技职业学院
关键词：
鸭甲型肝炎病毒;3D基因;原核表达;抗血清
摘要：
根据血清1型鸭甲型肝炎病毒(DHAV-1)SH株3D基因序列设计并合成1对特异性引物,通过RT-PCR方法扩增3D基因,将其克隆入原核表达载体p ET-32a,转化大肠杆菌BL21(DE3),在IPTG的诱导培养下重组蛋白获得了成功表达。SDS-PAGE显示重组蛋白的分子量约为68 k D,Western blot分析显示该重组蛋白能与多聚组氨酸标签单抗发生特异性反应。将切胶后纯化的目的蛋白免疫ICR小鼠,制备针对重组蛋白的多抗血清,Western blot结果显示制备的抗血清能够与DHAV-1感染的SPF鸡胚尿囊液总蛋白发生特异反应。以上结果表明,3D基因在大肠杆菌中获得了成功表达,且制备的多抗血清可以用于3D蛋白的检测,为3D蛋白的功能研究奠定了基础。
语种：
中文
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Characterization of Three Bacillus cereus Strains Involved in a Major Outbreak of Food Poisoning After Consumption of Fermented Black Beans (Douchi) in Yunan, China
作者：
Zhou, Guoping;Bester, Kai;Liao, Bin;Yang, Zushun;Jiang, Rongrong;...
期刊：
FOODBORNE PATHOGENS AND DISEASE ,2014年11(10):769-774 ISSN：1535-3141
通讯作者：
Hendriksen, Niels Bohse
作者机构：
[Jiang, Rongrong; Zhou, Guoping] Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan, Peoples R China.;[Bester, Kai; Hendriksen, Niels Bohse] Aarhus Univ, Dept Environm Sci, DK-4000 Roskilde, Denmark.;[Liao, Bin] Ctr Dis Control & Prevent Kunming, Kunming, Peoples R China.;[Yang, Zushun] Ctr Dis Control & Prevent Yunnan, Kunming, Peoples R China.;[Hendriksen, Niels Bohse] Aarhus Univ, Dept Environm Sci, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
通讯机构：
[Hendriksen, Niels Bohse] A;Aarhus Univ, Dept Environm Sci, Frederiksborgvej 399, DK-4000 Roskilde, Denmark.
关键词：
Bacillus;Food allergy;Pulses
摘要：
Three Bacillus cereus strains isolated from an outbreak of food poisoning caused by the consumption of fermented black beans (douchi) containing B. cereus is described. The outbreak involved 139 persons who had nausea, vomiting, and diarrhea. The strains were isolated from vomit and the unprepared douchi. Two of the strains produced the emetic toxin cereulide, as evidenced by polymerase chain reaction analysis for the presence of the nonribosomal synthetase cluster responsible for the synthesis of cereulide and by chemical analysis by high-performance liquid chromatography-mass spectrometry. These two strains belong to genetic group III of B. cereus, and multiple locus sequence typing revealed that the type was ST26, as a major part of B. cereus emetic strains. One of these strains produced significantly more cereulide at 37°C than the type cereulide producer (F4810/72), and it was also able to produce the toxin at 40°C and 42°C. The third strain belongs to genetic group IV, and it is a new multiple locus sequence type closely related to strains that are cytotoxic and enterotoxigenic. It possesses genes for hemolysin BL, nonhemolytic enterotoxin, and cytotoxin K2; however, it varies from the majority of strains possessing genes for hemolysin BL by not being hemolytic. Thus, two B. cereus strains producing the emetic toxin cereulide and a strain producing enterotoxins might have been involved in this food-poisoning incident caused by the consumption of a natural fermented food. The ability of one of the strains to produce cereulide at ≥37°C makes it possible that it is produced in the human gut in addition to occurring in the food. © Copyright 2014, Mary Ann Liebert, Inc.
语种：
英文
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A unique functional feature of the recombinant bovine prion protein fragment
作者：
Zhai, Ying;Li, Na;Zhang, Dachuan;Li, Qi;Zhou, Guoping;...
期刊：
WIT Transactions on Biomedicine and Health ,2014年19:331-338 ISSN：1743-3525
通讯作者：
Liu, Zhiguo
作者机构：
[Li, Qi; Zhang, Dachuan; Zhai, Ying; Zhou, Guoping; Li, Na; Liu, Zhiguo; Li, Rui] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
摘要：
To build a new research model of the bovine prion protein (PrP), we cloned and expressed a truncated fragment of the PrP and explore its functional feature. We obtained a recombinant protein of the PrP fragment (90 ∼231 amino acid). We analyzed the proteinase K resistance by enzymolysis, the binding force of the recombinant protein and bacteriophage Ff gene 5 protein (G5P) by affinity chromatography, and the enrichment effect of G5P on the recombinant PrP by Western blot. The recombinant protein was fully hydrolyzed by proteinase K. It specifically binds with G5P and the detectability limit was 0.5 pmol/L. The recombinant PrP showed similar sensitivity to proteinase K as the normal PrP, and high combination with G5P as the pathogenic PrP. Based on these excellent features, we can establish safe and effective separation and enrichment methods to provide feasible conditions for in vitro research and testing of pathogenic PrP. ©2014 WIT Press.
语种：
英文
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Detection of bacillus cereus containing voitoxin in food by loop-mediated isothermal amplification method
作者：
Liu, Zhiguo;Fang, Guoliang;Li, Qi* ;Zhou, Guoping;Li, Rui;...
期刊：
Journal of Food Safety ,2011年31(3):313-319 ISSN：0149-6085
通讯作者：
Li, Qi
作者机构：
[Li, Qi; Liu, Zhiguo; Li, Rui; Zhou, Guoping; Fu, Yunjie; Fang, Guoliang] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Qu, Shen; Li, He; Liu, Zhiguo] Huazhong Univ Sci & Technol, Dept Biochem & Mol Biol, Tongji Med Coll, Wuhan 430030, Peoples R China.
通讯机构：
[Li, Qi] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
关键词：
Bacillus cereus;Bacteria (microorganisms)
摘要：
Bacillus cereus strains containing voitoxin cereulide are pathogenic bacteria. The cereulide is produced by B. cereus strains containing plasmid pCER270. To establish a specific loop-mediated isothermal amplification (LAMP) method detecting pathogenic B. cereus, LAMP primer sets were designed based on 16SrDNA and pCER270 plasmid cesA sequence. The LAMP reaction was carried out under optimal conditions with 2mM of Mg2+ at 65C for 1h. Specificity of LAMP primers were validated by assaying 19 B. cereus strains with and without pCER270 plasmid and 42 non-B. cereus species. Detection limits of the LAMP assay were 1cfu/mL of B. cereus without pCER270 plasmid and 11cfu/mL of pathogenic B. cereus with pCER270 plasmid. Furthermore, liquid milk samples were detected by the LAMP method. The results showed that LAMP method is a reliable method used for rapid detection of B. cereus and its pathogenic strains of milk and other food stuff. © 2011 Wiley Periodicals, Inc..
语种：
英文
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Dot immunogold filtration assay for rapid detection of Sudan I residue in chili powder
作者：
Liu Zhiguo;Fu Yunjie;Li Qi;Li Kui;Chen Jiangyuan;...
期刊：
ITME 2011 - Proceedings: 2011 IEEE International Symposium on IT in Medicine and Education ,2011年2:628-632
通讯作者：
Liu, Z.(Zhiguo_1@126.com)
作者机构：
[Liu Zhiguo; Fu Yunjie; Li Qi; Li Kui; Chen Jiangyuan; Zhou Guoping; Li Rui; Liu Lieju] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
会议名称：
2011 IEEE International Symposium on IT in Medicine and Education
会议时间：
December 2011
会议地点：
Cuangzhou
会议论文集名称：
2011 IEEE International Symposium on IT in Medicine and Education
关键词：
Sudan I;polyclonal antibody;colloidal gold;dot immunogold filtration assay
摘要：
A method of Dot immunogold filtration assay for rapid detection of Sudan I residue in food was developed. Sudan I derivate-gelatin immunogen has been synthesized using the mixed anhydride method. Polyclonal antibodies were raised against the immunogen in rabbits. The purified antibody was immobilised on the nitrocellulose membrane as the test reagent. When the sample was added into the test device, the possible Sudan I in samples and the gold-labeled Sudan I-BSA conjugate which was added subsequently into the test device will competitively capture the immobilised antibody. The more Sudan I present in the samples, the more effective competition reaction with gold-labeled Sudan I-BSA occurred. A visible red dot shows a negative result in the read-out zone, and vice versa. Results showed that the detection limit was 0.8 g/mL in chili powder. Quantitative analysis of Sudan I could be achieved between 0.2 g/mL and 1.0 g/mL. Compared with HPLC methods, the results showed a good correlation with a square of correlation coefficient of 0.941. Low cross-reactivity was found among other Sudan dyes. The analysis could be completed in shorter time, suitable for rapid detect Sudan I residue in food and preliminary screening detections for abundant samples. ©2011 IEEE.<br/>
语种：
英文
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Occurrence of psychrotolerant Bacillus cereus group strains in ice creams
作者：
Zhou, Guoping;Zheng, Dasheng;Dou, Lina;Cai, Quanxin;Yuan, Zhiming*
期刊：
International Journal of Food Microbiology ,2010年137(2-3):143-146 ISSN：0168-1605
通讯作者：
Yuan, Zhiming
作者机构：
[Yuan, Zhiming; Cai, Quanxin; Zhou, Guoping; Zheng, Dasheng] Chinese Acad Sci, Wuhan Inst Virol, Ctr Appl & Environm Microbiol, Wuhan 430071, Peoples R China.;[Zhou, Guoping; Dou, Lina] Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Yuan, Zhiming] C;Chinese Acad Sci, Wuhan Inst Virol, Ctr Appl & Environm Microbiol, Wuhan 430071, Peoples R China.
关键词：
bacterial protein;DNA 16S;protein cspa;unclassified drug;article;Bacillus cereus;Bacillus thuringiensis;bacillus weihenstephanensis;bacterial gene;bacterial growth;bacterial strain;bacterium contamination;bacterium identification;controlled study;DNA fragmentation;DNA sequence;food contamination;food poisoning;ice cream;mesophilic bacterium;nonhuman;Bacillus cereus;Bacillus thuringiensis;Cold Temperature;DNA, Bacterial;DNA, Ribosomal;Genes, Bacterial;Humans;Ice Cream;RNA, Ribosomal, 16S;Sequence Analysis, DNA;United States;Bacillus cereus;Bacillus cereus group;Bacillus thuringiensis
摘要：
The occurrences of Bacillus cereus group strains in 40 ice cream samples were investigated. Among 109 isolated B. cereus group strains confirmed by 16S rDNA sequence analysis only 50 were identified as B. cereus and one as B. thuringiensis by using FDA (U.S. Food and Drug Administration) standard, indicating the two identification standards were highly inconsistent. Furthermore, the psychrotolerant growth properties and the occurrence of specific psychrotolerant genes of the isolates were also studied. Both psychrotolerant 16S rDNA fragments and enterotoxic genes could be detected among mesophilic and psychrotolerant strains. No relationship among psychrotolerance, presence of psychrotolerant 16S rDNA fragments and enterotoxic genes were found and the specific cspA fragment was only detected in a small fraction (9.5%) of the psychrotolerant isolates. One psychrotolerant isolate Bw2-1 was identified as B. weihenstephanensis, but no clear distinguishing characteristics between B. weihenstephanensis and psychrotolerant B. cereus were found. These results might be of importance for gaining further understanding of the growth properties of B. weihenstephanensis and psychrotolerant B. cereus as well as their contribution to food poisoning. © 2009 Elsevier B.V. All rights reserved.
语种：
英文
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The occurrence of Bacillus cereus, B. thuringiensis and B. mycoides in Chinese pasteurized full fat milk
作者：
Zhou, Guoping;Liu, Haizhou;He, Jing;Yuan, Yongming;Yuan, Zhiming*
期刊：
International Journal of Food Microbiology ,2008年121(2):195-200 ISSN：0168-1605
通讯作者：
Yuan, Zhiming
作者机构：
[Liu, Haizhou; Yuan, Zhiming; Zhou, Guoping; Yuan, Yongming] Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China.;[Zhou, Guoping; He, Jing] Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Liu, Haizhou; Zhou, Guoping; Yuan, Yongming] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.
通讯机构：
[Yuan, Zhiming] C;Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China.
关键词：
enterotoxin;milk fat;article;autumn;Bacillus;Bacillus cereus;Bacillus thuringiensis;bacterial count;bacterial strain;bacterium detection;bacterium identification;bacterium isolation;China;dairy product;food and drug administration;gene frequency;gene sequence;milk;nucleotide sequence;polymerase chain reaction;seasonal variation;spring;Animals;Bacillus;Bacillus cereus;Bacillus thuringiensis;China;Colony Count, Microbial;Consumer Product Safety;DNA Primers;Enterotoxins;Food Contamination;Food Microbiology;Food Preservation;Humans;Milk;Polymerase Chain Reaction;Seasons;Species Specificity;Bacillus cereus;Bacillus thuringiensis
摘要：
In 2006, a total of 54 samples of pasteurized full fat milk packaged in cartons were collected in spring and in autumn from chain supermarkets in Wuhan, China. The samples were examined and enumerated by MPN methods strictly according to guidelines laid out in US FDA/CFSAN BAM Chapter 14. Among 102 isolated B. cereus-like bacteria, 92 isolates were identified to be B. cereus, 9 B. thuringiensis and 1 B. mycoides. It was found that the occurrences of B. cereus were 71.4% and 33.3% in spring and in autumn samples respectively and the average count among the positive samples was 11.7 MPN/ml. The PCR detection results revealed that the enterotoxin genes hblA, hblC, hblD, nheA, nheB and nheC occurred in B. cereus isolates with frequencies of 37.0%, 66.3%, 71.7%, 71.7%, 62.0% and 71.7% respectively. Nine B. thuringiensis isolates were also identified from six pasteurized milk samples, and most of them harbored six enterotoxic genes and the insecticidal toxin cry1A gene. The single B. mycoides isolate harbored nheA and nheC genes. The data provides information for further evaluating the effect of B. cereus-like bacteria on food safety of Chinese milk products.
语种：
英文
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The residual occurrences of Bacillus thuringiensis biopesticides in food and beverages
作者：
Zhou, Guoping;Yan, Jianping;Zheng Dasheng;Zhou, Xiaohui;Yuan, Zhiming*
期刊：
International Journal of Food Microbiology ,2008年127(1-2):68-72 ISSN：0168-1605
通讯作者：
Yuan, Zhiming
作者机构：
[Yuan, Zhiming; Yan, Jianping; Zheng Dasheng; Zhou, Guoping] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.;[Zhou, Guoping; Zhou, Xiaohui] Wuhan Polytech Univ, Dept Bioengn & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Zhou, Guoping] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.
通讯机构：
[Yuan, Zhiming] C;Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
关键词：
kaiyan;lvpuan;pesticide;qiangdi;sutai;unclassified drug;article;Bacillus thuringiensis;bacterial gene;bacterial strain;bacterium isolate;bacterium isolation;beverage;commercial phenomena;controlled study;cry1a gene;food;fruit juice;ice cream;milk;nonhuman;peach;polyacrylamide gel electrophoresis;polymerase chain reaction;random amplified polymorphic DNA;serotyping;sporogenesis;tea;Animals;Bacillus thuringiensis;Bacterial Proteins;Beverages;DNA, Bacterial;Electrophoresis, Polyacrylamide Gel;Endotoxins;Food Contamination;Fruit;Gene Expression Regulation, Bacterial;Hemolysin Proteins;Humans;Insecticides;Milk;Random Amplified Polymorphic DNA Technique;Serotyping;Spores, Bacterial;Tea;Bacillus thuringiensis;Prunus persica
摘要：
In 2006, 54 pasteurized full fat milk samples, 40 ice-cream samples, and two green-tea beverage samples were analyzed and a total of 19 Bacillus thuringiensis-like strains were isolated, nine from seven pasteurized milks, one from an ice-cream with peach pulp and juice, and nine from two green-tea beverages. These strains were classified as B. thuringiensis, contained the cry1A gene and produced crystal inclusions during sporulation. All strains were characterized by a serotyping test, SDS-PAGE, random amplified polymorphic DNA, and enterotoxic gene PCR analysis. Most isolates produced bipyramidal crystals and belonged to serotypes H3a3b, H5a5b, or H7. Furthermore, two strains from pasteurized full fat milks and three strains from green-tea beverages were indistinguishable from the B. thuringiensis subsp. kurstaki strains isolated from commercial biopesticides (Kaiyan®, Qiangdi®, Lvpuan® and Sutai®), suggesting the residual occurrences of B. thuringiensis from biopesticides in food and beverages. © 2008 Elsevier B.V. All rights reserved.
语种：
英文
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五味子、木瓜果醋的研制
作者：
周帼萍;周容容;程正道;陈平
期刊：
中国酿造 ,2008年(4):94-96 ISSN：0254-5071
作者机构：
武汉工业学院,生物与制药工程系,湖北,武汉,430023;[程正道; 周帼萍; 周容容; 陈平] 武汉工业学院
关键词：
五味子;木瓜;果醋;复合发酵
摘要：
将木瓜和五味子干酒按2:1的比例混合,进行复合醋酸发酵。发酵220h时达到峰值,总酸度为9.4651g/100mL。制备成的复合果醋风味最佳,其色泽黄而微红,清亮透明,有木瓜的清香和淡淡的五味子果味。
语种：
中文
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热变性法提纯猪血SOD的工艺优化
作者：
刘晓红;李向东;刘婷;周帼萍
期刊：
武汉轻工大学学报 ,2008年27(1):8-10 ISSN：2095-7386
作者机构：
武汉工业学院,生物与制药工程系,湖北,武汉,430023;[刘晓红; 周帼萍; 李向东; 刘婷] 武汉工业学院
关键词：
猪血;热变性法
摘要：
在热变性法提取猪血中 SOD 工艺中,比较了Cu2+和Zn2+对 SOD 的保护作用和沉淀杂蛋白的作用,试验表明:这两种离子的硫酸盐对 SOD 的保护效应要优于氯盐;当Cu2+和Zn2+浓度分别为2mmol/L和3 mmol/L共同作用时对 SOD 的保护效果最好,SOD 比活力比空白对照组的提高了78.4%,杂蛋白沉淀量也提高了21.2%;而在这个Cu2+和Zn2+浓度下,热变性温度65 ℃时,SOD 的比活力最高,杂蛋白的沉淀效果也较好.
语种：
中文
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蜡状芽孢杆菌（Bacillus cereus）污染及其对食品安全的影响
作者：
周帼萍;袁志明
期刊：
食品科学 ,2007年28(3):357-361 ISSN：1002-6630
作者机构：
[周帼萍; 袁志明] 中国科学院武汉病毒研究所;中国科学院武汉病毒研究所 湖北武汉430071 武汉工业学院生物与制药工程系;湖北武汉430023
关键词：
蜡状芽孢杆菌;苏云金杆菌;食物安全;食物中毒;毒素
摘要：
蜡状芽孢杆菌是常见的食品污染菌，在工业化社会中正成为日益重要的食物病原菌。它能产生一种呕吐毒素和多种肠毒素，主要引发呕吐和腹泻型食物中毒。与蜡状芽孢杆菌同属的苏云金杆菌也能产生类似的肠毒素，近来发现它可能与食品中毒有关。我国应当加强对蜡状芽孢杆菌的监测和研究。
语种：
中文
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