作者： Yao, Yi-Lan;Shu, Chang;Feng, Ge;Wang, Qing;Yan, You-Yu;...

期刊： International Journal of Biological Macromolecules,2020年150:1162-1174 ISSN：0141-8130

通讯作者： Wang, Li-Mei

作者机构： [Wang, Hong-Xun; Shu, Chang; Feng, Ge; Wang, Qing; Zhang, Xi-Feng; Yao, Yi-Lan; Yan, You-Yu; Wang, Li-Mei] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Zhang, Xi-Feng] Qingdao Agr Univ, Coll Vet Med, Qingdao 266100, Peoples R China.

通讯机构： [Wang, Li-Mei] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

关键词： Antioxidant activity;Apoptosis;Cancer;P. fortuneana;Polysaccharides

摘要： This study used response surface methodology to determine the optimal conditions for extraction of polysaccharides from Pyracantha. fortuneana (PSPF), and studied the mechanism of PSPF-inducing apoptosis in human ovarian carcinoma Skov3 cells. Response surface methodology (RSM) were adopted to extract PSPF. The maximum value of polysaccharide yield was obtained under these optimal conditions. PSPF had good potential as an antioxidant. Exposure of cells to PSPF resulted in cytotoxicity through the induction of apoptosis, and the reactive oxygen species were increased, mitochondrial membrane potential decreased, DNA damage (detected as gamma- H2AX and RAD51 foci) was observed in Skov3 cells. In addition, PSPF could induce apoptosis of cancer cells. Therefore, PSPF should be explored as novel potential antioxidants and an anti-tumor drug in a clinical setting.

语种： 英文

作者： Feng, Ge;Zhang, Xi-Feng*

期刊： International Journal of Biological Macromolecules,2020年162:1227-1240 ISSN：0141-8130

通讯作者： Zhang, Xi-Feng

作者机构： [Feng, Ge; Zhang, Xi-Feng] Qingdao Agr Univ, Coll Vet Med, Qingdao, Peoples R China.;[Feng, Ge] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Zhang, Xi-Feng] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou 350002, Peoples R China.

通讯机构： [Zhang, Xi-Feng] Q;Qingdao Agr Univ, Coll Vet Med, Qingdao, Peoples R China.

关键词： Antioxidant activity;JC-1 staining;MTT;Polysaccharides iron;ROS

摘要： A water extraction and alcohol precipitation method was applied to extract polysaccharides from Codonopsis pilosula (CPP), response surface methodology was used to optimize the extraction conditions and synthesis of C. pilosula polysaccharide iron (CPPI), and the properties of CPPI were evaluated. The optimum extraction conditions for CPP were as follows: liquid-solid ratio of 29.39 mL/g, time of 1.25 h and temperature of 62.84 degrees C. The optimum synthesis conditions for CPPI were pH 8.9, temperature 70.30 degrees C and the ratio of citric acid to CPP1 of 2.95. An HPSEC-MALLS-RID system, UV spectroscopy, FT-IR spectroscopy and NMR were used for characterization of the polysaccharide. CPPI exhibited antioxidant activity in vitro and a relatively strong inhibitory effect on A2780 cells growth. After CPPI treatment, the reactive oxygen species increased, the mitochondrial membrane potential decreased, and DNA damage was observed in A2780 cells. Therefore, CPPI should be explored as a potential antioxidant and an antitumor drug in a clinical setting. (C) 2020 Elsevier B.V. All rights reserved.

语种： 英文

作者： Wang, Li-Mei;Yi, Yang;Yao, Yi-Lan;Feng, Ge;Shu, Chang;...

期刊： FOOD SCIENCE & NUTRITION,2019年7(1):293-301 ISSN：2048-7177

通讯作者： Zhang, Xi-Feng

作者机构： [Wang, Hong-Xun; Shu, Chang; Feng, Ge; Zhang, Xi-Feng; Wang, Li-Mei; Yao, Yi-Lan] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Wang, Hong-Xun; Yi, Yang; Wang, Li-Mei] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan, Hubei, Peoples R China.;[Wang, Hong-Xun; Yi, Yang; Wang, Li-Mei] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ China, Wuhan, Hubei, Peoples R China.;[Yi, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Zhang, Xi-Feng] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.

关键词： acid-sensing ion channels;Asic2a;Asic4;methylation;morris water maze;walnut oil

摘要： <jats:title>Abstract</jats:title><jats:p>Although Walnut oil (<jats:styled-content style="fixed-case">WO</jats:styled-content>) has been reported to enhance cognitive function, the underlying molecular mechanisms are not well understood. This study was designed to assess the effects of <jats:styled-content style="fixed-case">WO</jats:styled-content> on spatial memory in rats through modulation of the expression of acid‐sensing ion channel genes, <jats:italic>Asic2a</jats:italic> and <jats:italic>Asic4</jats:italic>. To investigate the effect of <jats:styled-content style="fixed-case">WO</jats:styled-content> on cognitive performance, we supplemented the diet of female rats with <jats:styled-content style="fixed-case">WO</jats:styled-content>. The results showed that supplementation with <jats:styled-content style="fixed-case">WO</jats:styled-content> at doses of 2.2 and 11gkg<jats:sup>−1</jats:sup>day<jats:sup>−1</jats:sup> significantly improved learning and memory. In vitro treatment of rat hippocampal neuronal cells with appropriate doses of <jats:styled-content style="fixed-case">WO</jats:styled-content> revealed a significant increase in the expression of <jats:italic>Asic2a</jats:italic> and <jats:italic>Asic</jats:italic>4 in a dose‐dependent manner at both the <jats:styled-content style="fixed-case">mRNA</jats:styled-content> and protein levels. We conclude that <jats:styled-content style="fixed-case">WO</jats:styled-content> intake might help to prevent cognitive decline, particularly in the elderly, and that <jats:styled-content style="fixed-case">ASIC</jats:styled-content> genes in neurons can be the targets of compounds contained in the oil.</jats:p>

语种： 英文

作者： Li, Jian;Bai, Ding-Ping;Zhang, Xi-Feng*

期刊： RSC Advances,2019年9(37):21513-21517 ISSN：2046-2069

通讯作者： Zhang, Xi-Feng

作者机构： [Bai, Ding-Ping; Li, Jian] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou 350002, Fujian, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Zhang, Xi-Feng] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

摘要： Due to the lack of a Panax japonicus var. major reference genome, we assembled a reference transcriptome from P. japonicus C. A. Mey transcriptome sequencing data, and 203 283 unigenes were obtained. In this study, with the assistance from the Trinity, Bowtie2 and SAMtools softwares, 218 m465 single nucleotide polymorphisms (SNPs) were identified by mapping the Illumina sequences to the reference transcriptome. The SNP forms included 126 262 transformations and 92 203 transversions. A large number of SNP loci were associated with triterpenoid saponin synthesis: 54 SNPs were associated with cytochrome P450, one with glycosyl transferase and 94 with the biosynthesis of the triterpenoid saponin backbone. © 2019 The Royal Society of Chemistry.

语种： 英文

作者： Liu, Xue-Lian;Wu, Rui-Ying;Sun, Xiao-Feng;Cheng, Shun-Feng;Zhang, Rui-Qian;...

期刊： INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018年14(3):294-305 ISSN：1449-2288

通讯作者： Li, Lan

作者机构： [Zhang, Rui-Qian; Liu, Xue-Lian; Zhang, Tian-Yu; Zhao, Yong; Shen, Wei; Li, Lan; Cheng, Shun-Feng] Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.;[Shen, Wei; Wu, Rui-Ying] Qingdao Univ, Qingdao Womens & Childrens Hosp, Ctr Reprod Med, Qingdao 266034, Peoples R China.;[Zhao, Yong; Shen, Wei; Sun, Xiao-Feng; Li, Lan; Cheng, Shun-Feng] Qingdao Agr Univ, Inst Reprod Sci, Coll Life Sci, Qingdao 266109, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Li, Lan] Q;Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.;Qingdao Agr Univ, Inst Reprod Sci, Coll Life Sci, Qingdao 266109, Peoples R China.

关键词： *DNA damage;*Genomic stability;*Granulosa cells;*Zearalenone

摘要： Zearalenone (ZEA) a metabolite of Fusarium fungi is commonly found on moldy grains. Because it can competitively combine to estrogen receptor to disrupt estrogenic signaling it has been reported to have serious adverse effects on animal reproduction systems. In order to explore the genotoxic effects of ZEA exposure on ovarian somatic cells porcine granulosa cells were exposed to 10 μM and 30 μM ZEA for 24 or 72 h in vitro. The results showed that ZEA exposure for 24 h remarkably reduced the proliferation of porcine granulosa cells in a dose-dependent manner as determined by MTT analysis and flow cytometry. Furthermore exposure to ZEA for 72 h induced apoptosis and RNA sequence analysis also revealed that the expression of apoptosis related genes were altered. RT-qPCR immunofluorescence and western blot analysis further confirmed the expression of DNA damage and repair related genes (γ-H2AX BRCA1 RAD51 and PRKDC) were increased in ZEA exposed granulosa cells. When the estrogen antagonist tamoxifen was added with ZEA in the culture medium the DNA damage and repairment by ZEA returned to normal level. Collectively these results illustrate that ZEA disrupts genome stability and inhibits growth of porcine granulosa cells via the estrogen receptors which may promote granulosa cell apoptosis when the DNA repair system is not enough to rescue this serious damage. © Ivyspring International Publisher.

语种： 英文

作者： Wang, Hualin;Cai, Yazheng;Shao, Yang;Zhang, Xifeng;Li, Na;...

期刊： International Journal of Molecular Sciences,2018年19(5):1325 ISSN：1661-6596

通讯作者： Liu, Zhiguo

作者机构： [Wang, Hualin; Liu, Zhiguo; Li, Na; Shao, Yang; Zhang, Xifeng; Zhang, Hongyu; Cai, Yazheng] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 30023, Hubei, Peoples R China.

通讯机构： [Liu, Zhiguo] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 30023, Hubei, Peoples R China.

关键词： 3(or 17)beta hydroxysteroid dehydrogenase;beta actin;cholesterol;cholesterol monooxygenase (side chain cleaving);cryptochrome 1;cryptochrome 2;estradiol;fish oil;omega 3 fatty acid;PER1 protein;PER2 protein;peroxisome proliferator activated receptor;retinoid related orphan receptor alpha;sirtuin 1;testosterone;testosterone 17beta dehydrogenase;transcription factor ARNTL;transcription factor CLOCK;triacylglycerol;omega 3 fatty acid;testosterone;animal experiment;animal model;animal tissue;apoptosis;Article;body weight;circadian rhythm;controlled study;down regulation;drug mechanism;gene expression;genital system disease;lipid diet;male;mouse;nonhuman;protein expression;real time polymerase chain reaction;reverse transcription polymerase chain reaction;seminiferous tubule;testosterone synthesis;TUNEL assay;Western blotting;animal;biosynthesis;C57BL mouse;drug effect;dyslipidemia;genetics;male infertility;metabolism;testis;Animals;Apoptosis;Circadian Rhythm;Diet, High-Fat;Dyslipidemias;Fatty Acids, Omega-3;Infertility, Male;Male;Mice;Mice, Inbred C57BL;Testis;Testosterone

摘要： The present study aims to investigate the protective effects of ω-3 polyunsaturated fatty acids (ω-3PUFAs) against high-fat diet induced male mouse reproductive dysfunction and to explore circadian regulation mechanisms. Male C57BL/6 mice were randomly divided into three groups and fed a normal chow diet (control group, CON), a high-fat diet (HFD group) or a HFD supplemented with fish oil (FO group) for 12 weeks. After 12 weeks of feeding, the body weight and the ratio of perinephric and epididymal fat weight to body weight were significantly higher in the HFD group compared with the CON group. The supplement of fish oil rich in ω-3PUFAs only slightly reduced the HFD-induced obesity but remarkably ameliorated HFD-induced dyslipidemia, sexual hormones disorder, testicle lesions and germ cell apoptosis. Fish oil supplementation restored the expression of steroid synthesis associated genes in HFD fed mouse and flattened the HFD-induced oscillations in circadian genes’ expression. Fish oil supplementation prevented HFD-induced male mouse reproductive dysfunction and modified the rhythmic expression of testosterone synthesis related genes. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Cheng, Shun-Feng;Li, Ling;Li, Bo;Liu, Jing-Cai;Lai, Fang-Nong;...

期刊： Molecular & Cellular Toxicology,2018年14(2):143-153 ISSN：1738-642X

通讯作者： Li, Lan

作者机构： [Liu, Jing-Cai; Lai, Fang-Nong; Zhao, Yong; Shen, Wei; Li, Lan; Cheng, Shun-Feng] Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.;[Li, Ling] Tengzhou Peoples Hosp, Tengzhou 277500, Peoples R China.;[Li, Bo] Chengguo Stn Anim Husb & Vet, Laizhou 261437, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Li, Lan] Q;Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.

关键词： DEHP;Mammary gland cells;Epigenetics;DNA methylation

摘要： Backgrounds: Di-(2-ethylhexyl) phthalate (DEHP), as an endocrine-disrupting chemical (EDC), is widely used in plasticizer and other productions. Ubiquitous human exposure to DEHP has been proposed to be a potential risk to public health. Developmental exposure to DEHP could alter epigenetic programming and result in adult-onset disease. Methods: In this study, we investigated whether DEHP exposure to pregnant mice affected epigenetic changes as a result of increase in breast cancer incidence. Results: Our results showed that the expression of total 143 epigenetics-related genes in mammary gland cells, have no significantly altered after short time and low-dose treated with DEHP from 0.5 days post-coitum (dpc) to 3.5 dpc of pregnant mice. DNA methylation status of some neoplastic development genes, such as EGFr, Esr1, Pgr, Fos and Rassf5 also had no obvious change. Conclusion: These finding showed no impact of DEHP on the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in pregnant mouse mammary gland cells. © 2018, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media B.V., part of Springer Nature.

语种： 英文

作者： Bai, Ding-Ping;Lin, Xin-Yu;Huang, Yi-Fan;Zhang, Xi-Feng*

期刊： International Journal of Molecular Sciences,2018年19(11):3299 ISSN：1661-6596

通讯作者： Zhang, Xi-Feng

作者机构： [Bai, Ding-Ping; Huang, Yi-Fan; Lin, Xin-Yu] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou 350002, Fujian, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Zhang, Xi-Feng] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： amphotericin B lipid complex;carbon nanoparticle;carbon nanotube;dendrimer;fullerene derivative;gold nanoparticle;graphene oxide;graphite;liposome;magnetic nanoparticle;metal nanoparticle;micellar nanoparticle;nanodiamond;nanohorn;nanomaterial;nanoparticle;polymeric nanoparticle;silver nanoparticle;ultrasmall superparamagnetic iron oxide;unclassified drug;zinc oxide nanoparticle;nanomaterial;animal health;antiangiogenic activity;antibacterial activity;antifungal activity;antiinflammatory activity;antineoplastic activity;antiviral activity;bacterial survival;biocompatibility;candidiasis;cell viability;controlled drug release;cryptococcosis;drug bioavailability;drug delivery system;drug formulation;drug penetration;drug safety;ecosystem restoration;Enterobacter;histoplasmosis;human;immunosuppressive treatment;Klebsiella;nanotechnology;nonhuman;particle size;pharmacokinetic parameters;physical chemistry;polymerization;Review;Staphylococcus aureus;Streptococcus agalactiae;theranostic nanomedicine;transmission electron microscopy;vaccine immunogenicity;veterinary medicine;wound healing;chemistry;procedures;theranostic nanomedicine;veterinary medicine;Nanostructures;Theranostic Nanomedicine;Veterinary Medicine

摘要： Nanoscience and nanotechnology shows immense interest in various areas of research and applications, including biotechnology, biomedical sciences, nanomedicine, and veterinary medicine. Studies and application of nanotechnology was explored very extensively in the human medical field and also studies undertaken in rodents extensively, still either studies or applications in veterinary medicine is not up to the level when compared to applications to human beings. The application in veterinary medicine and animal production is still relatively innovative. Recently, in the era of health care technologies, Veterinary Medicine also entered into a new phase and incredible transformations. Nanotechnology has tremendous and potential influence not only the way we live, but also on the way that we practice veterinary medicine and increase the safety of domestic animals, production, and income to the farmers through use of nanomaterials. The current status and advancements of nanotechnology is being used to enhance the animal growth promotion, and production. To achieve these, nanoparticles are used as alternative antimicrobial agents to overcome the usage alarming rate of antibiotics, detection of pathogenic bacteria, and also nanoparticles being used as drug delivery agents as new drug and vaccine candidates with improved characteristics and performance, diagnostic, therapeutic, feed additive, nutrient delivery, biocidal agents, reproductive aids, and finally to increase the quality of food using various kinds of functionalized nanoparticles, such as liposomes, polymeric nanoparticles, dendrimers, micellar nanoparticles, and metal nanoparticles. It seems that nanotechnology is ideal for veterinary applications in terms of cost and the availability of resources. The main focus of this review is describes some of the important current and future principal aspects of involvement of nanotechnology in Veterinary Medicine. However, we are not intended to cover the entire scenario of Veterinary Medicine, despite this review is to provide a glimpse at potential important targets of nanotechnology in the field of Veterinary Medicine. Considering the strong potential of the interaction between the nanotechnology and Veterinary Medicine, the aim of this review is to provide a concise description of the advances of nanotechnology in Veterinary Medicine, in terms of their potential application of various kinds of nanoparticles, secondly we discussed role of nanomaterials in animal health and production, and finally we discussed conclusion and future perspectives of nanotechnology in veterinary medicine. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhang, Xi-Feng;Huang, Feng-Hua;Zhang, Guo-Liang;Bai, Ding-Ping;Massimo, De Felici;...

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2017年12:7551-7575 ISSN：1176-9114

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Huang, Feng-Hua; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Zhang, Xi-Feng] Qingdao Agr Univ, Inst Reprod Sci, Qingdao, Peoples R China.;[Zhang, Guo-Liang] Dong EE Jiao Co Ltd, Natl Engn Res Ctr Gelatin Based Tradit Chinese Me, Donge, Shandong, Peoples R China.;[Bai, Ding-Ping; Huang, Yi-Fan] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou, Fujian, Peoples R China.;[Massimo, De Felici] Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, 120 Gwangjin Gu, Seoul 143701, South Korea.

关键词： graphene;trichostatin;cytotoxicity;reactive oxygen species;apoptosis;DNA fragmentation;double-strand DNA breaks

摘要： Background: Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). Methods: The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. Results: AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis. Conclusion: This study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression. © 2017 Zhang et al.

语种： 英文

作者： Liu, Jing-Cai;Lai, Fang-Nong;Li, Ling;Sun, Xiao-Feng;Cheng, Shun-Feng;...

期刊： CELL DEATH & DISEASE,2017年8(8):e2966 ISSN：2041-4889

通讯作者： Shen, Wei

作者机构： [Liu, Jing-Cai; Lai, Fang-Nong; Ge, Wei; Sun, Xiao-Feng; Shen, Wei; Li, Lan; Cheng, Shun-Feng; Wang, Yu-Feng] Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.;[Li, Ling] Tengzhou Peoples Hosp, Tengzhou 277500, Peoples R China.;[Sun, Xiao-Feng] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[De Felici, Massimo] Univ Roma Tor Vergata, Dept Biomed & Prevent, I-00133 Rome, Italy.

通讯机构： [Shen, Wei] Q;Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China.

会议名称： 中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”

会议时间： 2017-08-25

会议地点： 中国安徽合肥

会议论文集名称： 中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”论文集

关键词： DEHP;Mice;Oogenesis;Meiotic entry;DNA damage

摘要： <jats:title>Abstract</jats:title><jats:p>Di (2-ethylhexyl) phthalate (DEHP), is the most common member of the class of phthalates that are used as plasticizers and have become common environmental contaminants. A number of studies have shown that DEHP exposure impacts reproductive health in both male and female mammals by acting as an estrogen analog. Here, we investigated the effects of DEHP on meiotic progression of fetal mouse oocytes by using an <jats:italic>in vitro</jats:italic> model of ovarian tissue culture. The results showed that 10 or 100 <jats:italic>μ</jats:italic>M DEHP exposure inhibited the progression of oocytes throughout meiotic prophase I, specifically from the pachytene to diplotene stages. DEHP possibly impairs the ability to repair DNA double-strand breaks induced by meiotic recombination and as a consequence activates a pachytene check point. At later stages, such defects led to an increased number of oocytes showing apoptotic markers (TUNEL staining, expression of pro-apoptotic genes), resulting in reduced oocyte survival, gap junctions, and follicle assembly in the ovarian tissues. Microarray analysis of ovarian tissues exposed to DEHP showed altered expression of several genes including some involved in apoptosis and gonad development. The expression changes of some genes clustered in cell-cell communication and signal transduction, along with plasma membrane, extracellular matrix and ion channel function classes, were dependent on the DEHP concentration. Together, these results bring new support to the notion that exposure to DEHP during gestation might exert deleterious effects on ovary development, perturbing germ cell meiosis and the expression of genes involved in a wide range of biological processes including ovary development.</jats:p>

语种： 英文

作者： Lai, Fang-Nong;Liu, Jing-Cai;Li, Lan;Ma, Jun-Yu;Liu, Xue-Lian;...

期刊： Archives of Toxicology,2017年91(3):1279-1292 ISSN：0340-5761

通讯作者： Shen, Wei

作者机构： [Liu, Jing-Cai; Lai, Fang-Nong] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China.;[Liu, Jing-Cai; Lai, Fang-Nong; Ma, Jun-Yu; Liu, Xue-Lian; Shen, Wei; Li, Lan; Zhang, Xi-Feng] Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Chen, Hong; Li, Lan] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China.;[Liu, Yu-Ping] Qingdao Agr Univ, Hosp Sch, Qingdao 266109, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

通讯机构： [Shen, Wei] Q;Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.

关键词： Di (2-ethylhexyl) phthalate;Pre-puberty mice;Folliculogenesis;Steroidogenesis

摘要： Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer which is widely used in the manufacture of plastics. As a common environmental contaminant and recognized endocrine disrupting chemical, DEHP is able to deregulate the functions of a variety of tissues, including the reproductive system both in males and females. In order to investigate the possible effects of DEHP on the first wave of folliculogenesis, occurring in the mouse ovary postnatally, mice were administered 20 or 40μg/kg DEHP through intraperitoneal injection at days 5, 10 and 15 post partum (dpp). Following DEHP treatment the gene expression profile of control and exposed ovaries was compared by microarray analyses at 20dpp. We found that in the exposed ovaries DEHP significantly altered the transcript levels of several immune response and steroidogenesis associated genes. In particular, DEHP significantly decreased the expression of genes essential for androgen synthesis by theca cells including Lhcgr, Cyp17a1, Star and Ldlr. Immunohistochemistry and immune flow cytometry confirmed reduced expression of LHCGR and CYP17A1 proteins in the exposed theca cells. These effects were associated to a significant reduction in ovarian concentrations of progesterone, 17β-estradiol and androstenedione along with a reduction of LH in the serum. Although we did not find a significant reduction of the number of primary, secondary or antral follicles in the DEHP exposed ovaries when compared to controls, we did observe that theca cells showed an altered structure of the nuclear envelope, fewer mitochondria, and mitochondria with a reduced number of cristae. Collectively, these results demonstrate a deleterious effect of DEHP exposure on ovarian steroidogenesis during the first wave of folliculogenesis that could potentially affect the correct establishment of the hypothalamic-pituitary-ovarian axis and the onset of puberty. © 2016, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： Bai, Ding-Ping;Zhang, Xi-Feng;Zhang, Guo-Liang;Huang, Yi-Fan;Gurunathan, Sangiliyandi*

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2017年12:6521-6535 ISSN：1176-9114

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Bai, Ding-Ping; Huang, Yi-Fan] Fujian Agr & Forestry Univ, Fujian Key Lab Tradit Chinese Vet Med & Anim Hlth, Fuzhou, Fujian, Peoples R China.;[Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Zhang, Guo-Liang] Dong E E Jiao Co Ltd, Liaocheng, Shandong, Peoples R China.;[Zhang, Guo-Liang] Natl Engn Res Ctr Gelatin Based Tradit Chinese Me, Liaocheng, Shandong, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

关键词： zinc oxide nanoparticles;human ovarian cancer cells SKOV3;mitochondrial membrane potential;apoptosis;DNA fragmentation;autophagy

摘要： Background: Zinc oxide nanoparticles (ZnO NPs) are frequently used in industrial products such as paint, surface coating, and cosmetics, and recently, they have been explored in biologic and biomedical applications. Therefore, this study was undertaken to investigate the effect of ZnO NPs on cytotoxicity, apoptosis, and autophagy in human ovarian cancer cells (SKOV3). Methods: ZnO NPs with a crystalline size of 20 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The cytotoxicity apoptosis, and autophagy were examined using a series of cellular assays. Results: Exposure of cells to ZnO NPs resulted in a dose-dependent loss of cell viability, and the characteristic apoptotic features such as rounding and loss of adherence, enhanced reactive oxygen species generation, and loss of mitochondrial membrane potential were observed in the ZnO NP-treated cells. Furthermore, the cells treated with ZnO NPs showed significant double-strand DNA breaks, which are gained evidences from significant number of γ-H2AX and Rad51 expressed cells. ZnO NP-treated cells showed upregulation of p53 and LC3, indicating that ZnO NPs are able to upregulate apoptosis and autophagy. Finally, the Western blot analysis revealed upregulation of Bax, caspase-9, Rad51, γ-H2AX, p53, and LC3 and downregulation of Bcl-2. Conclusion: The study findings demonstrated that the ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in human ovarian cells through reactive oxygen species generation and oxidative stress. Therefore, this study suggests that ZnO NPs are suitable and inherent anticancer agents due to their several favorable characteristic features including favorable band gap, electrostatic charge, surface chemistry, and potentiation of redox cycling cascades. © 2017 Bai et al.

语种： 英文

作者： Zhang, Xi-Feng;Gurunathan, Sangiliyandi*

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2016年11:3655-3675 ISSN：1176-9114

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul 143701, South Korea.

关键词： apoptosis;autophagy;cell viability;caspase activity;ovarian cancer;salinomycin;silver nanoparticles

摘要： Combination of salinomycin and silver nanoparticles enhances apoptosis and autophagy in human ovarian cancer cells: an effective anticancer therapy Xi-Feng Zhang,1 Sangiliyandi Gurunathan2 1College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People&rsquo;s Republic of China; 2Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, South Korea Abstract: Ovarian cancer is one of the most important malignancies, and the origin, detection, and pathogenesis of epithelial ovarian cancer remain elusive. Although many cancer drugs have been developed to dramatically reduce the size of tumors, most cancers eventually relapse, posing a critical problem to overcome. Hence, it is necessary to identify possible alternative therapeutic approaches to reduce the mortality rate of this devastating disease. To identify alternative approaches, we first synthesized silver nanoparticles (AgNPs) using a novel bacterium called Bacillus clausii. The synthesized AgNPs were homogenous and spherical in shape, with an average size of 16&ndash;20&nbsp;nm, which are known to cause cytotoxicity in various types of human cancer cells, whereas salinomycin (Sal) is able to kill cancer stem cells. Therefore, we selected both Sal and AgNPs to study their combined effect on apoptosis and autophagy in ovarian cancer cells. The cells treated with either Sal or AgNPs showed a dose-dependent effect with inhibitory concentration (IC)-50 values of 6.0&nbsp;&micro;M and 8&nbsp;&micro;g/mL for Sal and AgNPs, respectively. To determine the combination effect, we measured the IC25 values of both Sal and AgNPs (3.0&nbsp;&micro;M and 4&nbsp;&micro;g/mL), which showed a more dramatic inhibitory effect on cell viability and cell morphology than either Sal or AgNPs alone. The combination of Sal and AgNPs had more pronounced effect on cytotoxicity and expression of apoptotic genes and also significantly induced the accumulation of autophagolysosomes, which was associated with mitochondrial dysfunction and loss of cell viability. Our data show a strong synergistic interaction between Sal and AgNPs in tested cancer cells. The combination treatment increased the therapeutic potential and demonstrated the relevant targeted therapy for the treatment of ovarian cancer. Furthermore, we provide, for the first time, a mode of action for Sal and AgNPs in ovarian cancer cells: enhanced apoptosis and autophagy. Keywords: apoptosis, autophagy, cell viability, caspase activity, ovarian cancer, salinomycin, silver nanoparticles

语种： 英文

作者： Han, Zhe;Yan, Qi;Ge, Wei;Liu, Zhi-Guo;Gurunathan, Sangiliyandi;...

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2016年11:5187-5203 ISSN：1176-9114

通讯作者： Zhang, Xi-Feng

作者机构： [Yan, Qi; Liu, Zhi-Guo; Han, Zhe; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Ge, Wei; Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul, South Korea.;[De Felici, Massimo] Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.

通讯机构： [Zhang, Xi-Feng] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

关键词： ZnO nanoparticle;Sertoli cells;Leydig cells;mice

摘要： Cytotoxic effects of ZnO nanoparticles on mouse testicular cells Zhe Han,1,* Qi Yan,1,* Wei Ge,2 Zhi-Guo Liu,1 Sangiliyandi Gurunathan,3 Massimo De Felici,4 Wei Shen,2 Xi-Feng Zhang1 1College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China; 2Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, People’s Republic of China; 3Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea; 4Department of Biomedicine and Prevention, University of Rome “Tor Vergata”, Rome, Italy *These authors contributed equally tothis work Background: Nanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model. Methods: ZnO NPs with a crystalline size of 70 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and atomic force microscopy. The cytotoxicity of the ZnO NPs was examined in vitro on Leydig cell and Sertoli cell lines, and in vivo on the testes of CD1 mice injected with single doses of ZnO NPs.Results: ZnO NPs were internalized by Leydig cells and Sertoli cells, and this resulted in cytotoxicity in a time- and dose-dependent manner through the induction of apoptosis. Apoptosis likely occurred as a consequence of DNA damage (detected as γ-H2AX and RAD51 foci) caused by increase in reactive oxygen species associated with loss of mitochondrial membrane potential. In addition, injection of ZnO NPs in male mice caused structural alterations in the seminiferous epithelium and sperm abnormalities.Conclusion: These results demonstrate that ZnO NPs have the potential to induce apoptosis in testicular cells likely through DNA damage caused by reactive oxygen species, with possible adverse consequences for spermatogenesis and therefore, male fertility. This suggests that evaluating the potential impacts of engineered NPs is essential prior to their mass production, to address both the environmental and human health concerns and also to develop sustainable and safer nanomaterials. Keywords: ZnO nanoparticle, Sertoli cells, Leydig cells, mice

语种： 英文

作者： Zhang, Xi-Feng;Liu, Zhi-Guo;Shen, Wei;Gurunathan, Sangiliyandi*

期刊： International Journal of Molecular Sciences,2016年17(9):1534 ISSN：1661-6596

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Liu, Zhi-Guo; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

关键词： angiogenesis inhibitor;silver nanoparticle;angiogenesis inhibitor;antiinfective agent;antiinflammatory agent;antineoplastic agent;metal nanoparticle;silver;antibacterial activity;antifungal activity;antiinflammatory activity;antineoplastic activity;antiviral activity;atomic force microscopy;biosensor;drug activity;drug mechanism;drug synthesis;gene therapy;human;infrared spectroscopy;nonhuman;photon correlation spectroscopy;Review;scanning electron microscopy;surface plasmon resonance;transmission electron microscopy;ultraviolet spectroscopy;X ray diffraction;X ray photoelectron spectroscopy;chemistry;green chemistry;Angiogenesis Inhibitors;Anti-Bacterial Agents;Anti-Inflammatory Agents;Antineoplastic Agents;Green Chemistry Technology;Humans;Metal Nanoparticles;Silver

摘要： Recent advances in nanoscience and nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials among several metallic nanoparticles that are involved in biomedical applications. AgNPs play an important role in nanoscience and nanotechnology, particularly in nanomedicine. Although several noble metals have been used for various purposes, AgNPs have been focused on potential applications in cancer diagnosis and therapy. In this review, we discuss the synthesis of AgNPs using physical, chemical, and biological methods. We also discuss the properties of AgNPs and methods for their characterization. More importantly, we extensively discuss the multifunctional bio-applications of AgNPs; for example, as antibacterial, antifungal, antiviral, anti-inflammatory, anti-angiogenic, and anti-cancer agents, and the mechanism of the anti-cancer activity of AgNPs. In addition, we discuss therapeutic approaches and challenges for cancer therapy using AgNPs. Finally, we conclude by discussing the future perspective of AgNPs. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhang, Xi-Feng;Shen, Wei;Gurunathan, Sangiliyandi*

期刊： International Journal of Molecular Sciences,2016年17(10):1603 ISSN：1661-6596

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

关键词： antiinfective agent;antineoplastic agent;lactate dehydrogenase;mitogen activated protein kinase;silver nanoparticle;metal nanoparticle;reactive oxygen metabolite;silver;angiogenesis;apoptosis;biocompatibility;biological activity;cell growth;cell migration;cell proliferation;cell survival;cell viability;cellular stress response;cytotoxicity;endothelium cell;fibroblast;gene expression;genotoxicity;HeLa cell line;human;keratinocyte;macrophage;nerve cell;neurotoxicity;nonhuman;Review;signal transduction;stem cell;animal;chemistry;cytology;DNA damage;drug effects;epithelium cell;metabolism;Animals;Cell Survival;DNA Damage;Endothelial Cells;Epithelial Cells;Humans;Macrophages;Metal Nanoparticles;Neurons;Reactive Oxygen Species;Silver;Stem Cells

摘要： Silver nanoparticles (AgNPs) have attracted increased interest and are currently used in various industries including medicine, cosmetics, textiles, electronics, and pharmaceuticals, owing to their unique physical and chemical properties, particularly as antimicrobial and anticancer agents. Recently, several studies have reported both beneficial and toxic effects of AgNPs on various prokaryotic and eukaryotic systems. To develop nanoparticles for mediated therapy, several laboratories have used a variety of cell lines under in vitro conditions to evaluate the properties, mode of action, differential responses, and mechanisms of action of AgNPs. In vitro models are simple, cost-effective, rapid, and can be used to easily assess efficacy and performance. The cytotoxicity, genotoxicity, and biocompatibility of AgNPs depend on many factors such as size, shape, surface charge, surface coating, solubility, concentration, surface functionalization, distribution of particles, mode of entry, mode of action, growth media, exposure time, and cell type. Cellular responses to AgNPs are different in each cell type and depend on the physical and chemical nature of AgNPs. This review evaluates significant contributions to the literature on biological applications of AgNPs. It begins with an introduction to AgNPs, with particular attention to their overall impact on cellular effects. The main objective of this review is to elucidate the reasons for different cell types exhibiting differential responses to nanoparticles even when they possess similar size, shape, and other parameters. Firstly, we discuss the cellular effects of AgNPs on a variety of cell lines; Secondly, we discuss the mechanisms of action of AgNPs in various cellular systems, and try to elucidate how AgNPs interact with different mammalian cell lines and produce significant effects; Finally, we discuss the cellular activation of various signaling molecules in response to AgNPs, and conclude with future perspectives on research into AgNPs. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhang, Xi-Feng;Gurunathan, Sangiliyandi*

期刊： INTERNATIONAL JOURNAL OF NANOMEDICINE,2016年11:6635-6649 ISSN：1176-9114

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.

关键词： biocompatibility;graphene oxide;nicotinamide;reduced graphene oxide;tight junction proteins;alkaline phosphatase

摘要： Biofabrication of a novel biomolecule-assisted reduced graphene oxide: an excellent biocompatible nanomaterial Xi-Feng Zhang,1 Sangiliyandi Gurunathan2 1College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People&rsquo;s Republic of China; 2Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, Republic of Korea Abstract: Graphene has been shown much interest, both in academics and industry due to its extraordinary physical, chemical, and biological proprieties. It shows great promises in biotechnological and biomedical applications as an antibacterial and anticancer agent, nanocarrier, sensor, etc. However, many studies demonstrated the toxicity of graphene in several cell lines, which is an obstacle to its use in biomedical applications. In this study, to improve the biocompatibility of graphene, we used nicotinamide (NAM) as a reducing and stabilizing agent to catalyze the reduction of graphene oxide (GO) to reduced graphene oxide (rGO). The resulted smaller-sized GO (NAM-rGO) showed excellent biocompatibility with mouse embryonic fibroblast cells, evidenced by various cellular assays. Furthermore, NAM-rGO had no effect on mitochondrial membrane permeability and caspase-3 activity compared to GO. Reverse transcription polymerase chain reaction analysis allowed us to identify the molecular mechanisms responsible for NAM-rGO-induced biocompatibility. NAM-rGO significantly induced the expression of genes encoding tight junction proteins (TJPs) such as zona occludens-1 (Tjp1) and claudins (Cldn3) without any effect on the expression of cytoskeleton proteins. Furthermore, NAM-rGO enhances the expression of alkaline phosphatase (ALP) gene, and it does this in a time-dependent manner. Overall, our study depicted the molecular mechanisms underlying NAM-rGO biocompatibility depending on upregulation of TJPs and ALP. This potential quality of graphene could be used in diverse applications including tissue regeneration and tissue engineering. Keywords: biocompatibility, graphene oxide, nicotinamide, reduced graphene oxide, tight junction proteins, alkaline phosphatase

语种： 英文

作者： Zhang, Xi-Feng;Shen, Wei;Gurunathan, Sangiliyandi*

期刊： Molecules,2016年21(6):731 ISSN：1420-3049

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul 143701, South Korea.

关键词： antioxidant;biomaterial;gold;metal nanoparticle;reactive oxygen metabolite;adverse effects;chemistry;cold;drug effects;Escherichia coli;growth, development and aging;heat;human;metabolism;oxidative stress;synthesis;Antioxidants;Biocompatible Materials;Cold Temperature;Escherichia coli;Gold;Hot Temperature;Humans;Metal Nanoparticles;Oxidative Stress;Reactive Oxygen Species

摘要： Due to their unique physical, chemical, and optical properties, gold nanoparticles (AuNPs) have recently attracted much interest in the field of nanomedicine, especially in the areas of cancer diagnosis and photothermal therapy. Because of the enormous potential of these nanoparticles, various physical, chemical, and biological methods have been adopted for their synthesis. Synthetic antioxidants are dangerous to human health. Thus, the search for effective, nontoxic natural compounds with effective antioxidative properties is essential. Although AuNPs have been studied for use in various biological applications, exploration of AuNPs as antioxidants capable of inhibiting oxidative stress induced by heat and cold stress is still warranted. Therefore, one goal of our study was to produce biocompatible AuNPs using biological methods that are simple, nontoxic, biocompatible, and environmentally friendly. Next, we aimed to assess the antioxidative effect of AuNPs against oxidative stress induced by cold and heat in Escherichia coli, which is a suitable model for stress responses involving AuNPs. The response of aerobically grown E. coli cells to cold and heat stress was found to be similar to the oxidative stress response. Upon exposure to cold and heat stress, the viability and metabolic activity of E. coli was significantly reduced compared to the control. In addition, levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and leakage of proteins and sugars were significantly elevated, and the levels of lactate dehydrogenase activity (LDH) and adenosine triphosphate (ATP) significantly lowered compared to in the control. Concomitantly, AuNPs ameliorated cold and heat-induced oxidative stress responses by increasing the expression of antioxidants, including glutathione (GSH), glutathione S-transferase (GST), super oxide dismutase (SOD), and catalase (CAT). These consistent physiology and biochemical data suggest that AuNPs can ameliorate cold and heat stress-induced oxidative damage in E. coli. Our results indicate that AuNPs may be effective antioxidants. However, further studies are needed to confirm the role of AuNPs as antioxidative agents, as well as their mechanism of action. © 2016 by the authors; licensee MDPI.

语种： 英文

作者： Zhang, Xi-Feng;Yan, Qi;Shen, Wei;Gurunathan, Sangiliyandi*

期刊： International Journal of Molecular Sciences,2016年17(8):1354- ISSN：1422-0067

通讯作者： Gurunathan, Sangiliyandi

作者机构： [Yan, Qi; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul 143701, South Korea.

通讯机构： [Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul 143701, South Korea.

关键词： caspase 3 inhibitor;glutathione;histone deacetylase inhibitor;lactate dehydrogenase;messenger RNA;palladium nanoparticle;saponin;superoxide dismutase;trichostatin A;caspase 3;histone deacetylase;hydroxamic acid;nanoparticle;palladium;trichostatin A;apoptosis;Article;cell viability;cytotoxicity;DNA fragmentation;female;human;human cell;human tissue;infrared spectroscopy;mitochondrial membrane potential;molecularly targeted therapy;oxidative stress;real time polymerase chain reaction;reverse transcription polymerase chain reaction;TUNEL assay;uterine cervix cancer;apoptosis;breast tumor;cell survival;chemistry;drug effects;enzyme activation;HeLa cell line;metabolism;uterine cervix tumor;Apoptosis;Breast Neoplasms;Caspase 3;Cell Survival;Enzyme Activation;Female;HeLa Cells;Histone Deacetylases;Humans;Hydroxamic Acids;Membrane Potential, Mitochondrial;Nanoparticles;Oxidative Stress;Palladium;Uterine Cervical Neoplasms

摘要： Cervical cancer ranks seventh overall among all types of cancer in women. Although several treatments, including radiation, surgery and chemotherapy, are available to eradicate or reduce the size of cancer, many cancers eventually relapse. Thus, it is essential to identify possible alternative therapeutic approaches for cancer. We sought to identify alternative and effective therapeutic approaches, by first synthesizing palladium nanoparticles (PdNPs), using a novel biomolecule called saponin. The synthesized PdNPs were characterized by several analytical techniques. They were significantly spherical in shape, with an average size of 5 nm. Recently, PdNPs gained much interest in various therapies of cancer cells. Similarly, histone deacetylase inhibitors are known to play a vital role in anti-proliferative activity, gene expression, cell cycle arrest, differentiation and apoptosis in various cancer cells. Therefore, we selected trichostatin A (TSA) and PdNPs and studied their combined effect on apoptosis in cervical cancer cells. Cells treated with either TSA or PdNPs showed a dose-dependent effect on cell viability. The combinatorial effect, tested with 50 nM TSA and 50 nMPdNPs, had a more dramatic inhibitory effect on cell viability, than either TSA or PdNPs alone. The combination of TSA and PdNPs had a more pronounced effect on cytotoxicity, oxidative stress, mitochondrial membrane potential (MMP), caspase-3/9 activity and expression of pro-and anti-apoptotic genes. Our data show a strong synergistic interaction between TSA and PdNPs in cervical cancer cells. The combinatorial treatment increased the therapeutic potential and demonstrated relevant targeted therapy for cervical cancer. Furthermore, we provide the first evidence for the combinatory effect and cytotoxicity mechanism of TSA and PdNPs in cervical cancer cells. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhang, Teng;Shen, Wei;De Felici, Massimo*;Zhang, Xi-Feng*

期刊： ENVIRONMENTAL AND MOLECULAR MUTAGENESIS,2016年57(8):579-588 ISSN：0893-6692

通讯作者： Zhang, Xi-Feng;De Felici, Massimo

作者机构： [Zhang, Teng; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Zhang, Teng; Shen, Wei] Qingdao Agr Univ, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[De Felici, Massimo] Univ Roma Tor Vergata, Dept Biomed & Prevent, I-00133 Rome, Italy.

通讯机构： [Zhang, Xi-Feng] W;[De Felici, Massimo] U;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;Univ Roma Tor Vergata, Dept Biomed & Prevent, I-00133 Rome, Italy.

关键词： di(2-ethylhexyl)phthalate;folliculogenesis;germ cells;endocrine disruptors

摘要： Primordial follicle formation and the subsequent transition of follicles through primary and secondary stages constitute crucial events of oogenesis. In particular, in mammals, defects in the processes that precede and accompany the formation of the primordial follicle pool can affect the size of this population significantly, while alterations in follicle activation, growth and maturation can result in premature depletion of the follicle reserve or cause follicle arrest at immature stages. Over the last decade, in vitro and in vivo approaches have been used to provide evidence that exposure to di(2-ethylhexyl)phthalate(DEHP), the most widely used plasticizer, has a deleterious effect on various stages of folliculogenesis in rodents. There is growing concern, supported by epidemiological and experimental data, that DEHP may have similar effects in women. This article reviews the evidence, with particular reference to our own findings, that DEHP may actually exert a variety of adverse effects on mammalian folliculogenesis from early to final stages of oogenesis, including altered development of the primordial germ cells, impaired fetal oocyte survival and meiotic progression, reduced oocyte nest breakdown, acceleration of primordial follicle activation, altered follicle steroidogenesis and increased follicle atresia. These effects can cause serious complications for reproductive and nonreproductive women's health. In addition, emerging data indicate that phthalates, including DEHP, may cause subtle epigenetic changes in germ cells that can be transmitted to subsequent generations, with potential negative effects on human health. Environ. Mol. Mutagen. 57:589–604, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

语种： 英文