作者： 樊庆涛;罗微;刘梁;赵玲;刘华英;...

期刊： 食品研究与开发,2022年43(03):117-122 ISSN：1005-6521

作者机构： 武汉轻工大学生命科学与技术学院,湖北武汉430023;湖北来凤腾升香料化工公司,湖北恩施445700;[子文杰] 深圳市樱落科技有限公司,广东深圳518000;[刘华英] 湖北来凤腾升香料化工有限公司;[刘梁; 陈新; 樊庆涛; 赵玲; 罗微] 武汉轻工大学

关键词： 来凤藤茶;二氢杨梅素;大孔树脂;纯化工艺;高效液相色谱

摘要： 采用乙酸乙酯萃取的方式对来凤藤茶提取液预处理,利用树脂法对萃取液中的二氢杨梅素进行纯化.以二氢杨梅素纯度为指标,选用4种大孔树脂对萃取液进行静态吸附与动态吸附试验.结果表明:在静态吸附试验中,HPD-100大孔吸附树脂对萃取液中的二氢杨梅素吸附量可达到14.1 mg/g,解吸率可达到87％;在动态吸附试验中,HPD-100大孔吸附树脂通过浓度为30％的乙醇溶液洗脱,洗脱液浓缩干燥后可获得纯度在95％以上的二氢杨梅素.

语种： 中文

作者： 申毓锦;何丹妮;杨博;赵玲

期刊： 武汉轻工大学学报,2022年41(4):31-34 ISSN：2095-7386

作者机构： 武汉轻工大学 生命科学与技术学院,武汉430023;[杨博] 武汉英普瑞医药科技有限公司,武汉430000;[申毓锦; 赵玲; 何丹妮] 武汉轻工大学

关键词： 泰泽司他;3-氨基-5-溴-2-甲基苯甲酸甲酯;合成;工艺优化

摘要： 对现有的泰泽司他(Tazemetostat)合成工艺进行了改进.以3-氨基-5-溴-2-甲基苯甲酸甲酯为原料,经过两次还原胺化反应,Suzuki偶联,水解,缩合5步反应得到目标化合物泰泽司他(Tazemetostat).结果显示:经1 H-NMR、LC-MS确认为目标化合物,总产率57.6％(以3-氨基-5-溴-2-甲基苯甲酸甲酯计),纯度达99.1％(HPLC法).该方法操作简单,无柱层析操作,收率、纯度高,适合工业化生产.

语种： 中文

作者： Wu, Shu Hui;Zhao, Yao Xin;Zhou, Jie;Ma, Guo Fei;Zhao, Ling*

期刊： Chemistry of Natural Compounds,2021年57(1):26-29 ISSN：0009-3130

通讯作者： Zhao, Ling

作者机构： [Zhao, Yao Xin; Wu, Shu Hui; Zhao, Ling] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Ma, Guo Fei; Zhou, Jie] Hubei Prov Key Lab Conservat Biol Shennongjia Gol, Shennongjia Natl Pk, Shennongjia 442411, Hubei, Peoples R China.

通讯机构： [Zhao, Ling] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

关键词： Alpinia officinarum;diarylheptanoid;officinine C;xanthine oxidase

摘要： A novel diarylheptanoid-flavonol dimer (1), named officinin C, was isolated from the rhizomes of Alpinia officinarum Hance. The structure elucidation was accomplished by HR-ESI-MS and 1D and 2D NMR methods. Its xanthine oxidase inhibitory activity was tested along with officinin A (2). At the concentration of 25 mu M, compounds 1 and 2 can reach 57 and 52% inhibition rates against xanthine oxidase.

语种： 英文

作者： 郭嘉鹏;罗微;章慧;刘梁;赵玲;...

期刊： 生物化工,2021年7(05):17-20 ISSN：2096-0387

作者机构： 武汉轻工大学 生命科学与技术学院,湖北武汉 430023;[刘华英] 湖北来凤腾升香料化工公司,湖北恩施 445000;[刘梁; 陈新; 章慧; 赵玲; 罗微; 郭嘉鹏] 武汉轻工大学

关键词： 来凤凤菊;多糖;纯化

摘要： 目的:研究来凤凤菊茎叶多糖的脱色纯化工艺.方法:以来凤凤菊茎叶为对象,选取HPD-100、D101、AB-8、HPD-400、DM130和ADS-7共6种树脂以及DEAE-52纤维素,以脱色率和保留率为指标,比较7种树脂在来凤凤菊茎叶多糖脱色方面的性能,以及OH-型、Cl-型和HCO3-型DEAE-52纤维素对多糖的纯化效果.结果:DEAE-52纤维素脱色和纯化效果最佳,脱色率为91.84％,保留率为91.66％,HCO3-型分离效果好,得到7个主要成分.结论:DEAE-52纤维素适用于来凤凤菊茎叶多糖脱色,HCO3-型的DEAE-52纤维素色谱法用于来凤凤菊茎叶多糖的脱色纯化效果好,值得进一步研究并应用于生产实践.

语种： 中文

作者： 吴淑慧;於洪建;於天;郑志刚;柯达;...

期刊： 现代药物与临床,2021年36(02):226-230 ISSN：1674-5515

作者机构： [吴淑慧; 赵玲] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[於洪建; 柯达; 郑志刚] Wuxi Century Biological Engineering Co., Ltd., Wuxi, 214000, China;[於天] Tianjin Yibeiyuan Natural Product Technology Co., Ltd., Tianjin, 300000, China

关键词： 茶黄素;高尿酸血症;尿酸;尿素氮;肌酐

摘要： 目的 研究茶黄素对高尿酸血症模型小鼠的降尿酸作用.方法 采用氧嗪酸钾诱导高尿酸血症小鼠模型,茶黄素低、中、高剂量(6、20、60 mg/kg)和别嘌呤(10 mg/kg)连续ig给药7 d,检测高尿酸血症小鼠血清尿酸(UA)、尿素氮(BUN)、肌酐(CRE)、天冬氨酸氨基转氨酶(AST)、丙氨酸氨基转氨酶(ALT)水平,评价茶黄素的降尿酸作用.结果 与对照组比较,模型组小鼠血清CRE、BUN、AST和ALT水平显著升高;与模型组相比,茶黄素中、高剂量组小鼠血清UA、CRE、BUN、AST、ALT水平显著降低(P<0.01).结论 茶黄素对于氧嗪酸钾诱导的高尿酸血症模型小鼠有良好的降尿酸作用.

语种： 中文

作者： 樊庆涛;孙湘沛;刘梁;赵玲;刘华英;...

期刊： 中国酿造,2021年40(05):134-139 ISSN：0254-5071

作者机构： [刘梁; 陈新; 孙湘沛; 樊庆涛; 赵玲] 武汉轻工大学 生命科学与技术学院,湖北 武汉 430023;[刘华英] 湖北来凤腾升香料化工公司,湖北 恩施 445700

关键词： 藤茶;二氢杨梅素;乳酸菌;流式细胞术

摘要： 该研究通过生长曲线和流式细胞术实验初步探索了藤茶中二氢杨梅素对嗜酸乳杆菌（Lactobacillus acidophilus）、德氏乳杆菌保加利亚亚种（Lactobacillus delbrueckii subsp. bulgaria）、嗜热链球菌（Streptococcus thermophilus）三种乳酸菌生长的影响。结果表明,二氢杨梅素添加量在100～1 000μg/mL时,对三种乳酸菌均有显著促进其生长并提高其菌液活菌数量的作用（P<0.05）;当二氢杨梅素添加量>1 000μg/mL时,对三种菌的生长和菌液中活菌的数量均表现出不同程度的抑制,其中德氏乳杆菌保加利亚亚种对二氢杨梅素最为敏感。在发酵后的菌液分析中,未明显观察到二氢杨梅素含量的降低,说明二氢杨梅素通过对生长环境进行调节影响乳酸菌生长。

语种： 中文

作者： 吴淑慧;胡淼淼;王薇;赵玲

期刊： 武汉轻工大学学报,2021年40(05):102-107 ISSN：2095-7386

作者机构： 武汉轻工大学 生命科学与技术学院 武汉430023;[胡淼淼; 赵玲; 王薇; 吴淑慧] 武汉轻工大学

关键词： 高良姜多糖;微波;提取;响应面法

摘要： 以高良姜根茎为原料,研究高良姜多糖的微波提取工艺。利用单因素实验和Box-Behnken响应面实验相结合,以多糖得率作为响应值,以料液比、提取时间、微波功率作为优化对象自变量,建立响应面回归模型,筛选出高良姜粗多糖的最佳微波提取工艺。最终得出高良姜多糖的最优微波提取条件是:料液比（g∶mL）1∶45,提取时间20 min,微波功率350 W。在此最佳提取工艺条件下,高良姜多糖的实际得率为12.34%,与模型预测值接近,该模型拟合程度较高。对高良姜多糖产率的主要影响因素从大到小依次为:微波功率＞提取时间＞料液比。采用微波提取技术提取高良姜多糖具有省力高效的优点,为后续高良姜多糖深入开发利用奠定了理论基础。

语种： 中文

作者： 王薇;胡淼淼;石光锐;赵玲

期刊： 武汉轻工大学学报,2021年40(04):87-92 ISSN：2095-7386

作者机构： [石光锐; 胡淼淼; 赵玲; 王薇] 武汉轻工大学生命科学与技术学院,武汉430023

关键词： 高良姜素;高良姜;提取纯化;D101大孔树脂

摘要： 为寻找一条适合工业化生产高良姜素的路线,本研究采用乙醇提取法、D101型大孔树脂柱色谱法和结晶法相结合的方法,并对各步骤的工艺参数进行了详细优化,成功得到高纯度高良姜素.首先用8倍量的85％乙醇水溶液(体积比)提取高良姜,回流3次,每次2h,得到粗提物,将提取液以1 BV(Bed Volume,树脂床体积)/h的流速在D101大孔树脂柱上负载15 BVs,再用85％乙醇溶液洗脱除去杂质,得到高良姜素粗品,最后以60％乙醇进行重结晶处理,即得纯度达95％高良姜素,经中试放大试验证明该工艺操作简便,稳定可行,为高良姜素工业化生产提供实验依据.

语种： 中文

作者： Zeng, Xiaofei;Sheng, Jiajing;Zhu, Fenglin;Wei, Tianzi;Zhao, Lingling;...

期刊： Biotechnology for Biofuels and Bioproducts,2020年13(1):1-14 ISSN：2731-3654

通讯作者： Diao, Ying;Jin, Surong

作者机构： [Diao, Ying; Zeng, Xiaofei] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Zeng, Xiaofei] Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China.;[Sheng, Jiajing] Nantong Univ, Sch Life Sci, Nantong 226019, Peoples R China.;[Hu, Zhongli; Hu, Xiaohu; Sheng, Jiajing; Zhao, Lingling; Zhu, Fenglin; Zhou, Fasong; Zheng, Xingfei] Wuhan Univ, Coll Life Sci, Hubei Lotus Engn Ctr, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China.;[Jin, Surong] Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Peoples R China.

通讯机构： [Diao, Ying; Jin, Surong] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Peoples R China.

关键词： Miscanthus × giganteus;Bioethanol;Lignin;Monolignol biosynthesis pathway;Monolignol biosynthetic genes;Transcription factors;Regulatory mechanism;Transcriptome analysis;Genetic engineering

摘要： Miscanthus × giganteus is widely recognized as a promising lignocellulosic biomass crop due to its advantages of high biomass production, low environmental impacts, and the potential to be cultivated on marginal land. However, the high costs of bioethanol production still limit the current commercialization of lignocellulosic bioethanol. The lignin in the cell wall and its by-products released in the pretreatment step is the main component inhibiting the enzymatic reactions in the saccharification and fermentation processes. Hence, genetic modification of the genes involved in lignin biosynthesis could be a feasible strategy to overcome this barrier by manipulating the lignin content and composition of M. × giganteus. For this purpose, the essential knowledge of these genes and understanding the underlying regulatory mechanisms in M. × giganteus is required. In this study, MgPAL1, MgPAL5, Mg4CL1, Mg4CL3, MgHCT1, MgHCT2, MgC3′H1, MgCCoAOMT1, MgCCoAOMT3, MgCCR1, MgCCR2, MgF5H, MgCOMT, and MgCAD were identified as the major monolignol biosynthetic genes in M. × giganteus based on genetic and transcriptional evidence. Among them, 12 genes were cloned and sequenced. By combining transcription factor binding site prediction and expression correlation analysis, MYB46, MYB61, MYB63, WRKY24, WRKY35, WRKY12, ERF021, ERF058, and ERF017 were inferred to regulate the expression of these genes directly. On the basis of these results, an integrated model was summarized to depict the monolignol biosynthesis pathway and the underlying regulatory mechanism in M. × giganteus. This study provides a list of potential gene targets for genetic improvement of lignocellulosic biomass quality of M. × giganteus, and reveals the genetic, transcriptional, and regulatory landscape of the monolignol biosynthesis pathway in M. × giganteus.

语种： 英文

作者： 阮文静;柯尊军;杨博;赵玲

期刊： 中国新药杂志,2020年29(7):822-825 ISSN：1003-3734

通讯作者： Zhao, L.

作者机构： [阮文静; 柯尊军; 赵玲] School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[杨博] Wuhan Ying Purui Pharmaceutical Technology Co., Ltd., Wuhan, 430014, China

通讯机构： [Zhao, L.] S;School of Biology and Pharmaceutical Engineering, China

关键词： 高效液相色谱法;达克替尼;含量测定;有关物质

摘要： 目的:建立测定达克替尼含量及有关物质的高效液相色谱法(high performance liquid chromatography, HPLC),以控制达克替尼的质量标准。方法:采用Agilent Eclipse XDB×C_(18)柱(250 mm×4.6 mm, 5 μm);流动相为乙酸铵水溶液(30 mmol·L~(-1))-甲醇(20∶80);流速: 1.0 mL·min~(-1);柱温为25 ℃;检测波长为252 nm;进样体积为20 μL。结果:本方法能较好地分离达克替尼和杂质峰,达克替尼浓度在48.40~242.0 μg·mL~(-1)浓度范围内,线性关系良好(r= 0.999 9);精密度、稳定性、重复性的RSD均<1%;回收率为99.79%, RSD= 0.39%(n= 9)。结论:该操作方法简便、快捷、重复性好,可用于达克替尼的含量测定。

语种： 中文

作者： Zhao, Yao-xin;Ruan, Wen-jing;Xue, Wen-lai;Zhao, Ling*

期刊： 亚太热带医药杂志：英文版,2019年12(12):565-573 ISSN：1995-7645

通讯作者： Zhao, Ling

作者机构： [Zhao, Yao-xin; Ruan, Wen-jing; Zhao, Ling; Xue, Wen-lai] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Peoples R China.

通讯机构： [Zhao, Ling] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Peoples R China.

关键词： Alpinia offcinarum Hance extract;Lung injury;Particulate matter

摘要： To evaluate the effect of Alpinia officinarum Hance (A. officinarum) extract on lung injury caused by particulate matter (PM). Methods: The Kunming mice were intranasally instilled with PM and treated with A. officinarum extract for 3 weeks. Bronchoalveolar lavage fluid, blood and lung samples were collected for biochemical, serological and histopathological studies. Results: Serological analysis showed that albumin levels, lactate dehydrogenase and alkaline phosphatase activities in bronchoalveolar lavage fluid were significantly reduced after administrations of 50, 100 and 200 mg/kg of A. officinarum extracts to the PM injured mice. Markers of oxidative stress, nitric oxide, malondialdehyde levels and nitric oxide synthase activities, were significantly decreased. Correspondingly, total superoxide dismutase activity was improved dramatically. The expressions of interleukin-6 and tumor necrosis factor alpha were also down-regulated obviously. In addition, pathological sections of lung tissue showed that A. officinarum could reduce the infiltration of inflammatory cells, pulmonary edema and pulmonary fibrosis. These results showed that A. officinarum extract could alleviate PM-induced lung injury via reducing the permeability of cell membranes in lung tissue, eliminating oxidative stress and relieving inflammatory response. Conclusions: A. officinarum extract was an efficient treatment for PM-induced lung injury ir mice, and it may be a promising therapeutic agent in future. © 2019 Wolters Kluwer Medknow Publications. All rights reserved.

语种： 英文

作者： Hu, Xiao-Yu;Li, Yun;Li, Long-Quan;Zheng, Yuan;Lv, Jia-Hong;...

期刊： BMJ OPEN,2018年8(4):e019974 ISSN：2044-6055

通讯作者： Zhao, Xiu-Ju（赵秀举）

作者机构： [Zhao, Xiu-Ju; Li, Yun; Li, Long-Quan; Lv, Jia-Hong; Huang, Shu-Chun; Zhang, Weinong; Zhao, Ling; Liu, Liang; Liu, Zhuiguo; Hu, Xiao-Yu; Zheng, Yuan] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan, Hubei, Peoples R China.;[Zhao, Xiu-Ju] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA.

通讯机构： [Zhao, Xiu-Ju] W;[Zhao, Xiu-Ju] C;[Zhao, Xiu-Ju] T;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan, Hubei, Peoples R China.

关键词： epidemiology;hepatology;public health

摘要： Objective Non-alcoholic fatty liver disease (NAFLD) is a major public health burden in China, and its prevalence is increasing. This study aimed to determine the risk factors and biomarkers of NAFLD. Design An observational cross-sectional primary survey. Setting Central China. Participants The study included 1479 participants aged over 18 and below 80 years, not currently being treated for cancer or infectious disease or no surgery in the previous year, and no history of cancer or an infectious disease. Participants underwent clinical examination, metabolomic assay and anthropometric assessment. Univariate and logistic regression analyses were used to evaluate associations between covariates and NAFLD. Main outcome measures Risk factors and metabolic biomarkers including sex, body mass index, hypertension, body fat ratio, blood triglycerides, blood fasting glucose, liver enzyme elevation, uric acid and oleic acid-hydroxy oleic acid (OAHOA). Results Data from the 447 participants (mean age 44.3±11.9 years) were analysed, and the prevalence of NAFLD was 24.7%. Male sex (OR 3.484, 95% CI 2.028 to 5.988), body mass index ≥24 kg/m 2 (OR 8.494, 95% CI 5.581 to 12.928), body fat ratio (≥25 for women, ≥20 for men) (OR 1.833, 95% CI 1.286 to 2.756), triglycerides ≥1.7 mmol/L (OR 1.340, 95% CI 1.006 to 1.785), fasting glucose ≥6.1 mmol/L (OR 3.324, 95% CI 1.888 to 5.850), blood pressure ≥140/90 mm Hg or antihypertensive drug treatment (OR 1.451, 95% CI 1.069 to 1.970), uric acid (≥357 μmol/L for women, ≥416 μmol/L for men) (OR 2.755, 95% CI 2.009 to 3.778) and OAHOA (<5 nmol/L) (OR 1.340, 95% CI 1.006 to 1.785) were independent predictors of NAFLD (all P<0.05). These results were verified by all 1479 participants. Conclusions NAFLD was common among the study participants. In particular, NAFLD was correlated with uric acid. We identified OAHOA as a novel marker of NAFLD prevalence. It provides a reference on the prevention of NAFLD and related metabolic diseases with the rapid urbanisation, technological advancement and population ageing in China over the recent decades. © 2018 Article author(s) (or their employer(s) unless otherwise stated in the text of the article). All rights reserved.

语种： 英文

作者： 陈旅翼;李赫宇;赵玲

期刊： 中草药,2016年47(22):4039-4042 ISSN：0253-2670

通讯作者： Zhao, L.

作者机构： [陈旅翼] College of Pharmacy, South-central University For Nationalities, Wuhan, 430743, China;[李赫宇] Tianjin Ubasic health Nutrition Co., Ltd., Tianjin, 300457, China;[赵玲] College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, China

通讯机构： [Zhao, L.] C;College of Biological and Pharmaceutical Engineering, China

关键词： 复合萃取物;降尿酸;量效关系;时效关系;葡萄籽;人参萃取物

摘要： 目的研究一种复合萃取物（主要由葡萄皮、葡萄籽、红酒萃取物及人参、枸杞萃取物组成）体内降尿酸作用的量效和时效关系。方法雄性昆明种小鼠随机分为对照组,模型组,别嘌醇组及复合萃取物高、中、低剂量（400、200、100 mg/kg）组。时效关系研究中,各组分别连续给药2、4、7、10、13、16、20 d后,ip氧嗪酸钾溶液20 m L/kg造模;量效关系研究中,各组连续ig给药7 d后,ip氧嗪酸钾溶液20 m L/kg造模;按照试剂盒方法分别测定小鼠血清及尿液中尿酸水平及肝组织中黄嘌呤氧化酶（XOD）活性。结果时效关系研究发现,复合萃取物组给药4 d即显示出明显的降尿酸活性,连续给药7 d后,其低、中、高3个剂量组均表现出显著的活性,且在连续20 d的实验过程中,小鼠生理和精神状态都非常好。量效关系研究表明,复合萃取物低剂量（100mg/kg）时即显示良好的降尿酸活性,并存在一定的量效关系,以高剂量组最为显著,且高剂量组还显示出显著的抑制肝脏XOD的活性。结论复合萃取物在体内具有良好的降尿酸活性,呈现一定的量效和时效关系,其降尿酸作用与抑制XOD活性有关。

语种： 中文

作者： 陈新;刘梁;赵玲

期刊： 食品研究与开发,2015年(08):5 ISSN：1005-6521

作者机构： 湖北省农产品加工协调创新中心武汉轻工大学生物与制药工程学院

摘要： <正>农产品不仅是人们日常食物的主要来源,同时还富含各类生物活性物质。种类丰富的农产品为新型、高效、成本低廉的保健品、营养食品、药品的开发提供了开阔的资源。在未来的功能食品和药品活性物质研究中,从农产品中寻找生物兼容性好、安全性高的活性物质已经成为科研工作者的一个研究重点。本课题组近年来一直围绕农产品中生物活性物质研究、开发与利用等方面开展工作,取得了一定的成果。主要建立了稳定的米糠多糖和米糠肤酶法提取、分离工艺,

语种： 中文

作者： Liu Dan;Qu Wei;Zhao Ling;Guan Fu-Qin;Liang Jing-Yu*

期刊： 中国天然药物,2014年12(2):139-141 ISSN：2095-6975

通讯作者： Liang Jing-Yu

作者机构： [Liang Jing-Yu; Qu Wei; Liu Dan] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Jiangsu, Peoples R China.;[Zhao Ling] Wuhan Polytech Univ, Coll Biotechnol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Guan Fu-Qin] Inst Bot, Nanjing 210014, Jiangsu, Peoples R China.;[Guan Fu-Qin] Chinese Acad Sci, Nanjing 210014, Jiangsu, Peoples R China.

通讯机构： [Liang Jing-Yu] C;China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Jiangsu, Peoples R China.

关键词： (4E)-1,7-diphenylhept-4-en-3-one;1,7-diphenyl-3,5-heptanedione;Alpinia officinarum Hance;alpinin B;diarylheptanoid

摘要： Aim: To study the chemical constituents of the rhizomes of Alpinia officinarum Hance. Method: Compounds were isolated by repeated column chromatography, and their structures were elucidated on the basis of spectral analysis. The cytotoxic activities of these compounds were evaluated with the T98G and B16F10 cell lines by the MTT assay. Results: A dimeric diarylheptanoid, named alpinin B (1), along with three known diarylheptanoids were obtained, and their structures were identified as alpinin B (1), 1, 7-diphenyl-3,5-heptanedione (2), (4E)-1, 7-diphenylhept-4-en-3-one (3) and (4E)-7- (4-hydroxyphenyl)-1-phenylhept-4-en-3-one (4). Conclusion: Compound 1 is a new dimeric diarylheptanoid. The biosynthetic pathway of 1 was speculated to originate from a Michael reaction between compounds 2 and 3. Compound 3 showed cytotoxicity against the human glioblastoma T98G cell line with IC50 of 27 μmol·L-1. © 2014 China Pharmaceutical University.

语种： 英文

作者： Chen, Lvyi*;Lan, Zhou;Ma, Shuwei;Zhao, Ling;Yang, Xinzhou

期刊： PLANTA MEDICA,2013年79(8):634-638 ISSN：0032-0943

通讯作者： Chen, Lvyi

作者机构： [Yang, Xinzhou; Chen, Lvyi] South Cent Univ Nationalities, Sch Pharm, Wuhan 430074, Peoples R China.;[Lan, Zhou] China Pharmaceut Univ, Dept Pharmacol Chinese Mat Med, Nanjing, Jiangsu, Peoples R China.;[Ma, Shuwei] Qiqihar Univ, Inst Chem & Chem Engn, Qiqihar, Peoples R China.;[Zhao, Ling] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Peoples R China.;[Chen, Lvyi] South Cent Univ Nationalities, Sch Pharm, 708 Min Zu Rd, Wuhan 430074, Peoples R China.

通讯机构： [Chen, Lvyi] S;South Cent Univ Nationalities, Sch Pharm, 708 Min Zu Rd, Wuhan 430074, Peoples R China.

关键词： Elaeagnus pungens;Elaeagnaceae;emodinol;gouty arthritis;interleukin 1 beta;monosodium urate crystals

摘要： A series of studies have recently demonstrated that the release of interleukin 1β induced by monosodium urate crystals is central to the experimental gouty arthritis. Elaeagnus pungens has been traditionally used for the treatment of gouty arthritis in China for more than thousands years. However, there is still little known about the active ingredients and mechanisms of E. pungens against gouty arthritis. Emodinol, as a major triterpene compound in E. pungens, has been seldom reported to have an effect on gouty arthritis. Therefore, the potential beneficial effects and mechanisms of emodinol on gouty arthritis were investigated in this study. Results showed that it significantly ameliorated the hyperalgesia, inflammation, and levels of multiple proinflammatory cytokines in monosodium urate crystals-treated mice. These findings elucidate that emodinol exhibits a prominent effect on improving symptoms of acute gouty arthritis induced by monosodium urate crystals through inhibiting the generation of proinflammatory cytokines.

语种： 英文

作者： 赵玲;赵春才;杨博;刘艳艳;施琳

期刊： 中国药房,2013年23(21):2007-2009 ISSN：1001-0408

作者机构： [赵玲] 武汉工业学院生物与制药工程学院;[赵春才; 刘艳艳; 施琳] 南京科瑞翔医药科技有限公司;[杨博] 南京安格医药化工有限公司

关键词： 盐酸阿莫地喹片;有关物质;含量测定;高效液相色谱法

摘要： 目的:建立测定盐酸阿莫地喹片含量和有关物质的方法。方法:采用高效液相色谱(HPLC)法,并与该制剂国家标准中的薄层色谱(TLC)法检测有关物质和紫外-可见分光光度法(UV)测定含量的结果进行比较。色谱柱为Insteril ODS-3,流动相为缓冲溶液[水-三乙胺-磷酸(100 ∶1 ∶0.5)]-甲醇(75 ∶25),流速为1.0 ml/min,检测波长为342 nm,柱温为30 ℃。结果:盐酸阿莫地喹检测质量浓度线性范围为10.06~100.55 μg/ml(r=0.999 9),平均回收率为99.56%,RSD=0.87%(n=9),定量限和检测限分别为6.7、0.4 ng。2种含量测定方法结果相似,RSD分别为0.60%、0.71%;2种有关物质测定方法中TLC法未检出杂质,HPLC法测定总杂质量为0.10%~0.13%。结论:所建立的方法准确、简便、可靠,能有效控制盐酸阿莫地喹片的质量,可考虑代替国家标准方法。

语种： 中文

作者： Zhao Ling;Chen Lv-Yi;Liang Jing-Yu*

期刊： 中国天然药物,2012年10(5):370-373 ISSN：2095-6975

通讯作者： Liang Jing-Yu

作者机构： [Zhao Ling] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Liang Jing-Yu] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China.;[Chen Lv-Yi] S Cent Univ Nationalities, Sch Pharm, Wuhan 430074, Peoples R China.

通讯机构： [Liang Jing-Yu] C;China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China.

关键词： Alpinia galanga;Zingiberaceae;Phenylpropanoids;Chemical constituent;Cytotoxic activity

摘要： Aim: To study the chemical constituents and bioactivity of the rhizomes of Alpinia galangal Willd. (Zingiberaceae). Methods: The chemical constituents were isolated using various chromatographic methods and crystallization, and the chemical structures were elucidated on the basis of spectral analysis. In addition, cytotoxic activities toward four tumor cell lines (HepG2, EC109, A549 and Vero) were tested by MTT method. Results: Six phenylpropanoids were obtained and their structures were identified as (S)-1'-ethoxy chavicol acetate (1), (E)-4-acetoxy cinnamyl ethyl ether (2), (E)-4-hydroxycinnamaldehyde (3), (E)-4-acetoxy cinnamyl alcohol (4), 4-acetoxy cinnamyl acetate (5), and 4, 4'[(2E, 2'E)-bis(prop-2-ene)-1, 1'-oxy]-diphenyl-7, 7'-diacetate (6). Conclusion: Compounds 1 and 2 were two new phenylpropanoids. Compound 5 shows selective cytotoxic activity on human lung adenocarcinoma cell A549 (IC50 19.35 μmol·L-1). © 2012 China Pharmaceutical University.

语种： 英文

作者： Liu, Dan;Qu, Wei;Zhao, Ling;Liang, Jing Yu*

期刊： 中国化学快报：英文版,2012年23(2):189-192 ISSN：1001-8417

通讯作者： Liang, Jing Yu

作者机构： [Liang, Jing Yu; Qu, Wei; Liu, Dan] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China.;[Zhao, Ling] Wuhan Polytech Univ, Coll Biotechnol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

通讯机构： [Liang, Jing Yu] C;China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China.

关键词： Alpinia officinarum Hance;Diarylheptanoid;Alpinin A

摘要： A novel dimeric diarylheptanoid, named alpinin A (1), along with two known compounds, 1,7-diphenyl-5-ol-3-heptanone and 7(4'-hydroxy-3'-methoxyphenyl)-1-phenyl-4-heptene-3-one, were isolated from the rhizomes of Alpinia officinarum Hance. The structure of compound 1 was elucidated on the basis of spectral analysis, including HR-IT-TOF-MS, 1D and 2D NMR. And a possible biogenetic pathway had been proposed for 1. (C) 2011 Published by Elsevier B.V. on behalf of Chinese Chemical Society.

语种： 英文

作者： Zhao, Ling*;Liang, Jing Yu;Qu, Wei

期刊： Chemistry of Natural Compounds,2012年48(5):836-838 ISSN：0009-3130

通讯作者： Zhao, Ling

作者机构： [Zhao, Ling] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Liang, Jing Yu; Qu, Wei] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Peoples R China.

通讯机构： [Zhao, Ling] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

关键词： Alpinia officinarum Hance;diarylheptanoid;officinine B

摘要： A novel dimeric diarylheptanoid (E)-3-[3-(3-methoxy-4-hydroxyphenyl)prop-1- enyl]-2,4,6-triphenethylpyridine (1), named officinine B, was isolated from the rhizomes of Alpinia officinarum Hance. Its structure was elucidated by spectral methods (1H NMR, 13C NMR, 1H-1H COSY, HMBC, and HR-ESI-MS). © 2012 Springer Science+Business Media New York.

语种： 英文