作者机构：
[Fen Teng] Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Longxia Hu; Jiaqing Wang; Yi Dai; Lihong Bao; Yun Yang; Tingting Jiao; Fei Luo; Yali Yang; Zixi Wang; Xiaoping Yang; Shengli Yang] Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Deying Hu; Yilan Liu; Yanhong Han; Xiaoping Ding] Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Wenbin Liu; Hongling Zhang; Qingzhou Cheng] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Deying Hu] D;[Wenbin Liu] C;Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
关键词：
attitude;knowledge;living will;malignant tumor;palliative care
摘要：
Given the suboptimal quality of end-of-life care among patients with cancer in China, promoting living wills is critical in this population. Living wills ensure that individuals can receive the medical care they desire during the terminal phase of an illness, maintain their dignity, and ultimately achieve a good death. However, current awareness and attitudes about living wills among Chinese patients with cancer remain unclear. We administered a questionnaire survey on living wills to patients with malignant tumors to assess their most important needs and increase understanding about living wills.
In this cross-sectional study using convenience sampling, inpatients with malignant tumors in Wuhan completed our questionnaire between July 2020 and June 2021. We collected patients' sociodemographic characteristics and details regarding their knowledge and attitudes about living wills.
Among 213 patients with malignant tumors, 114 (53.52%) had heard of living wills; 125 (58.69%) expressed their willingness to sign the “Five Wishes” living will document after learning about it through the questionnaire. Patients with malignant tumors had a high level of desire for the following living will items: the lives of family and friends return to normal as soon as possible after their death, maintaining personal hygiene and dignity, and remaining pain-free. The knowledge level of patients with malignant tumors was related to their educational level ( p < 0.05) and self-care ability ( p < 0.05).
Patients with malignant tumors have a high need for comfort, cleanliness, and pain relief in the terminal stages. Patients with a higher level of education and those with poorer self-care ability had greater knowledge and acceptance of living wills. Promotion can first be targeted toward more highly educated patients and can then be gradually expanded to other groups.
摘要：
Blended vegetable oil is considered to be a valuable product in the market owing to favourable taste and nutritional composition. The quantification of its contents has notable implications for protecting food safety and consumer interests. Thus, a rapid and non-destructive method is needed to analyse the composition of blended oil. This study established an analytical method combining Raman spectroscopy and prediction models to determine the content of olive oil in a mixture. Competitive adaptive reweighted sampling was employed to select feature bands attributed to β-carotene and unsaturated fatty acids. Various models were used to calculate the mixture proportion, and the importance of characteristic peak intensity affecting the prediction was evaluated via grey relational analysis. The random forest model exhibited superior performance in quantitative analysis, with RMSE and R 2 of 0.0447 and 0.9799, respectively. Overall, this approach was proven to effectively identify blended olive oils, exemplifying its potential in food authentication.
Blended vegetable oil is considered to be a valuable product in the market owing to favourable taste and nutritional composition. The quantification of its contents has notable implications for protecting food safety and consumer interests. Thus, a rapid and non-destructive method is needed to analyse the composition of blended oil. This study established an analytical method combining Raman spectroscopy and prediction models to determine the content of olive oil in a mixture. Competitive adaptive reweighted sampling was employed to select feature bands attributed to β-carotene and unsaturated fatty acids. Various models were used to calculate the mixture proportion, and the importance of characteristic peak intensity affecting the prediction was evaluated via grey relational analysis. The random forest model exhibited superior performance in quantitative analysis, with RMSE and R 2 of 0.0447 and 0.9799, respectively. Overall, this approach was proven to effectively identify blended olive oils, exemplifying its potential in food authentication.
期刊：
Journal of Pharmaceutical and Biomedical Analysis,2024年249:116397 ISSN：0731-7085
通讯作者：
Cheng, C
作者机构：
[Cheng, Qingzhou; Cheng, Chu; Zhou, Wei; Chen, Yulong] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.;[Xiao, Pengfeng] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing, Peoples R China.
通讯机构：
[Cheng, C ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.
关键词：
Decoding sequencing;High accuracy;Long read length;Rare mutations;Single-color fluorogenic DNA
摘要：
We proposed a single-color fluorogenic DNA decoding sequencing method designed to improve sequencing accuracy, increase read length and throughput, as well as decrease scanning time. This method involves the incorporation of a mixture of four types of 3’-O-modified nucleotide reversible terminators into each reaction. Among them, two nucleotides are labeled with the same fluorophore, while the remaining two are unlabeled. Only one nucleotide can be extended in each reaction, and an encoding that partially defines base composition can be obtained. Through cyclic interrogation of a template twice with different nucleotide combinations, two sets of encodings are sequentially obtained, enabling the determination of the sequence. We demonstrate the feasibility of this method using established sequencing chemistry, achieving a cycle efficiency of approximately 99.5 %. Notably, this strategy exhibits remarkable efficacy in the detection and correction of sequencing errors, achieving a theoretical error rate of 0.00016 % at a sequencing depth of ×2, which is lower than Sanger sequencing. This method is theoretically compatible with the existing sequencing-by-synthesis (SBS) platforms, and the instrument is simpler, which may facilitate further reductions in sequencing costs, thereby broadening its applications in biology and medicine. Moreover, we demonstrate the capability to detect known mutation sites using information from only a single sequencing run. We validate this approach by accurately identifying a mutation site in the human mitochondrial DNA.
We proposed a single-color fluorogenic DNA decoding sequencing method designed to improve sequencing accuracy, increase read length and throughput, as well as decrease scanning time. This method involves the incorporation of a mixture of four types of 3’-O-modified nucleotide reversible terminators into each reaction. Among them, two nucleotides are labeled with the same fluorophore, while the remaining two are unlabeled. Only one nucleotide can be extended in each reaction, and an encoding that partially defines base composition can be obtained. Through cyclic interrogation of a template twice with different nucleotide combinations, two sets of encodings are sequentially obtained, enabling the determination of the sequence. We demonstrate the feasibility of this method using established sequencing chemistry, achieving a cycle efficiency of approximately 99.5 %. Notably, this strategy exhibits remarkable efficacy in the detection and correction of sequencing errors, achieving a theoretical error rate of 0.00016 % at a sequencing depth of ×2, which is lower than Sanger sequencing. This method is theoretically compatible with the existing sequencing-by-synthesis (SBS) platforms, and the instrument is simpler, which may facilitate further reductions in sequencing costs, thereby broadening its applications in biology and medicine. Moreover, we demonstrate the capability to detect known mutation sites using information from only a single sequencing run. We validate this approach by accurately identifying a mutation site in the human mitochondrial DNA.
作者机构：
[Cheng, Qingzhou; Cheng, Chu; Zhou, Wei; Chen, Yulong; Liu, Wenbin] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.;[Zhang, Zhiling; Ye, Jingsi] Huaren Technol Co Ltd, Wuhu, Peoples R China.;[Xiao, Pengfeng] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing, Peoples R China.
通讯机构：
[Cheng, C ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.
摘要：
Eliminating errors in next-generation sequencing has proven to be challenging. Here we present a novel strategy for DNA sequencing, called correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry. This sequencing method involves introducing a mixture of natural nucleotide X, labeled unblocked nucleotide X ', 3 ' blocked nucleotide Y*, and labeled 3 ' blocked nucleotide Y* into each reaction cycle. By cyclically interrogating a template twice with different nucleotide combinations, two sets of base-encoding are sequentially obtained, enabling accurate deduction of base sequence. We demonstrate the remarkable efficacy of this approach in detecting and correcting sequencing errors, achieving a theoretical error rate of 0.0005%, which is twice as accurate as Sanger sequencing. Furthermore, we show the capability to detect known mutation sites using information from only a single sequencing run. The correctable two-color fluorogenic DNA decoding sequencing approach should enable accurate identification of extremely rare genomic variations in diverse applications in biology and medicine. A correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry.
通讯机构：
[Cheng, QZ ; Ding, H ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.;Wuhan Univ, Sch Pharmaceut Sci, 185 Donghu Rd, Wuhan 430072, Hubei, Peoples R China.
关键词：
Epilepsy;Luteolin;Neuronal apoptosis;Neuroinflammation;TLR4/I kappa B alpha/NF-kappa B pathway
摘要：
Epilepsy represents a prevalent neurological disorder in the population, and the existing antiepileptic drugs (AEDs) often fail to adequately control seizures. Inflammation is recognized as a pivotal factor in the pathophysiology of epilepsy. Luteolin, a natural flavonoid extract, possesses anti-inflammatory properties and exhibits promising neuroprotective activity. Nevertheless, the precise molecular mechanisms underlying the antiepileptic effects of luteolin remain elusive. In this study, we established a rat model of epilepsy using pentylenetetrazole (PTZ) to induce seizures. A series of behavioral experiments were conducted to assess behavioral abilities and cognitive function. Histological techniques, including HE staining, Nissl staining, and TUNEL staining, were employed to assess hippocampal neuronal damage. Additionally, Western blotting, RT-qPCR, and ELISA were utilized to analyze the expression levels of proteins involved in the TLR4/IκBα/NF-κB signaling pathway, transcription levels of apoptotic factors, and levels of inflammatory cytokines, respectively. Luteolin exhibited a dose-dependent reduction in seizure severity, prolonged the latency period of seizures, and shortened seizure duration. Furthermore, luteolin prevented hippocampal neuronal damage in PTZ-induced epileptic rats and partially restored behavioral function and learning and memory abilities. Lastly, PTZ kindling activated the TLR4/IκBα/NF-κB pathway, leading to elevated levels of the cytokines TNF-α, IL-6 and IL-1β, which were attenuated by luteolin. Luteolin exerted anticonvulsant and neuroprotective activities in the PTZ-induced epileptic model. Its mechanism was associated with the inhibition of the TLR4/IκBα/NF-κB pathway, alleviating the immune-inflammatory response in the post-epileptic hippocampus.
Epilepsy represents a prevalent neurological disorder in the population, and the existing antiepileptic drugs (AEDs) often fail to adequately control seizures. Inflammation is recognized as a pivotal factor in the pathophysiology of epilepsy. Luteolin, a natural flavonoid extract, possesses anti-inflammatory properties and exhibits promising neuroprotective activity. Nevertheless, the precise molecular mechanisms underlying the antiepileptic effects of luteolin remain elusive. In this study, we established a rat model of epilepsy using pentylenetetrazole (PTZ) to induce seizures. A series of behavioral experiments were conducted to assess behavioral abilities and cognitive function. Histological techniques, including HE staining, Nissl staining, and TUNEL staining, were employed to assess hippocampal neuronal damage. Additionally, Western blotting, RT-qPCR, and ELISA were utilized to analyze the expression levels of proteins involved in the TLR4/IκBα/NF-κB signaling pathway, transcription levels of apoptotic factors, and levels of inflammatory cytokines, respectively. Luteolin exhibited a dose-dependent reduction in seizure severity, prolonged the latency period of seizures, and shortened seizure duration. Furthermore, luteolin prevented hippocampal neuronal damage in PTZ-induced epileptic rats and partially restored behavioral function and learning and memory abilities. Lastly, PTZ kindling activated the TLR4/IκBα/NF-κB pathway, leading to elevated levels of the cytokines TNF-α, IL-6 and IL-1β, which were attenuated by luteolin. Luteolin exerted anticonvulsant and neuroprotective activities in the PTZ-induced epileptic model. Its mechanism was associated with the inhibition of the TLR4/IκBα/NF-κB pathway, alleviating the immune-inflammatory response in the post-epileptic hippocampus.
摘要：
Phthalides are a class of unique compounds such as ligustilide, butylphthalide and butyldenephthalide, which have shown to possess multiple bioactivities in new drug research and development. Phthalides are naturally distributed in different plants that have been utilized as herbal treatments for various ailments with a long history in Asia, Europe and North America. Their extensive biological activity has led to a dramatic increase in the study of phthalide compounds in recent years. This review summarizes the latest research progress of plant-derived phthalides, and a total of 133 phthalide compounds are described based on the characteristics of chemical structures. Pharmacological properties of plant-derived phthalides are associated with hemorheological improvement, vascular function modulation and central nervous system protection. Potential treatments for a variety of diseases mainly including cardio-cerebrovascular disorders and neurological complications such as Alzheimer's disease are also concluded. In addition, key metabolic pathways have been clearly elucidated. Further investigations on the molecular mechanisms involved in biological activity are recommended for offering new insights into profound comprehension of phthalide applications.
摘要：
<jats:p><jats:bold>Background:</jats:bold> Migraine is a common neurovascular disorder with typical throbbing and unilateral headaches, causing a considerable healthcare burden on the global economy. This research aims to prepare chitosan-alginate (CS-AL) nanoparticles (NPs) containing Foshousan oil (FSSO) and investigate its potential therapeutic effects on the treatment of migraine.</jats:p><jats:p><jats:bold>Methods:</jats:bold> FSSO-loaded CS-AL NPs were prepared by using the single emulsion solvent evaporation method. Lipopolysaccharide (LPS)-stimulated BV-2 cells and nitroglycerin (NTG)-induced migraine mice were further used to explore anti-migraine activities and potential mechanisms of this botanical drug.</jats:p><jats:p><jats:bold>Results:</jats:bold> FSSO-loaded CS-AL NPs (212.1 ± 5.2nm, 45.1 ± 6.2mV) had a well-defined spherical shape with prolonged drug release and good storage within 4weeks. FSSO and FSSO-loaded CS-AL NPs (5, 10, and 15μg/mL) showed anti-inflammatory activities in LPS-treated BV-2 cells via reducing the levels of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and nitric oxide (NO), but elevating interleukin-10 (IL-10) expressions. Moreover, FSSO-loaded CS-AL NPs (52 and 104mg/kg) raised pain thresholds against the hot stimulus and decreased acetic acid-induced writhing frequency and foot-licking duration in NTG-induced migraine mice. Compared with the model group, calcitonin gene-related peptide (CGRP) and NO levels were downregulated, but 5-hydroxytryptamine (5-HT) and endothelin (ET) levels were upregulated along with rebalanced ET/NO ratio, and vasomotor dysfunction was alleviated by promoting cerebral blood flow (CBF) in the FSSO-loaded CS-AL NPs (104mg/kg) group.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> FSSO-loaded CS-AL NPs could attenuate migraine via inhibiting neuroinflammation in LPS-stimulated BV-2 cells and regulating vasoactive substances in NTG-induced migraine mice. These findings suggest that the FSS formula may be exploited as new phytotherapy for treating migraine.</jats:p>
作者：
Wang, Xuan*;Zhang, Hongling;Cheng, Qingzhou;Chen, Jing;Qiu, Nan
期刊：
PROCEEDINGS OF THE 6TH INTERNATIONAL CONFERENCE ON ELECTRONIC, MECHANICAL, INFORMATION AND MANAGEMENT SOCIETY (EMIM),2016年40:1041-1045 ISSN：2352-538X
摘要：
Nowadays, an effective teaching platform is an important factor in the distance education. In the paper, the structure and functional modules of the teaching platform system based on web technology is studied comprehensively, and these functional modules of the teaching platform is introduced in detail. And the implementation process of the main technical and design principles is generated in the paper. The platform has some function of management and teaching in the education based on multi-layer structure.
