作者： Wang, Zhaobin;Li, Jia;Wang, Yu;Wang, Lei;Yin, Yuebang;...

期刊： Journal of Animal Science,2020年98(2) ISSN：1525-3163

通讯作者： Yang, Huansheng

作者机构： [Wang, Zhaobin; Li, Jia; Yin, Lanmei; Wang, Lei; Yin, Yulong; Yang, Huansheng] Hunan Normal Univ, Lab Anim Nutr & Human Hlth, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Coll Life Sci,Hunan Prov Key Lab Anim Intestinal, Changsha 410081, Hunan, Peoples R China.;[Wang, Zhaobin; Li, Jia; Wang, Lei; Yin, Yuebang; Yin, Yulong; Yang, Huansheng] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg,Minist Agr, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Inst Subtrop Agr,Sci Observing & Expt Stn Anim Nu, Changsha 410125, Hunan, Peoples R China.;[Wang, Yu] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Yang, Huansheng] H;[Yang, Huansheng] C;Hunan Normal Univ, Lab Anim Nutr & Human Hlth, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Coll Life Sci,Hunan Prov Key Lab Anim Intestinal, Changsha 410081, Hunan, Peoples R China.;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg,Minist Agr, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Inst Subtrop Agr,Sci Observing & Expt Stn Anim Nu, Changsha 410125, Hunan, Peoples R China.

关键词： Vitamin A;enzyme activity;growth performance;intestinal development;intestinal stem cells;weaning piglets

摘要： Vitamin A (VA) is an important nutrient for weaning piglets. It plays a significant role in the normal formation, development, and maintenance of epithelial cells. Previous studies have shown that VA supplements could improve the host's intestinal barrier function. Therefore, we hypothesized that VA supplements can affect intestinal function in weaned piglets by regulating intestinal stem cells. Thirty-two 21-day-old weaned [(Yorkshire x Landrace) x Duroc] piglets with an average weight of 8.34 +/- 0.13 kg were randomly divided into four treatment groups, with (i) 2 mg/kg (control), (ii) 4 mg/kg, (iii) 8 mg/kg and (iv) 16 mg/kg doses of VA, respectively. The experiment lasted for 14 days. Weaned piglets were given ad libitum access to food and water during the test. The ADG (Linear, P = 0.020) and G: F (Linear, P = 0.005) of the piglets were found to increase significantly from day 8 to 14. The Lgr5+ gene expression (P = 0.012) in the jejunum mucosa of the 16 mg/kg VA group was increased. The jejunum villus height (P = 0.027) and villi surface area (P = 0.035) were significantly increased in the 4 mg/kg VA treatment group. The crypt depth increased significantly in the 4 and 8 mg/kg VA treatment groups (Quadratic, P = 0.043), and the ratios of villus height to crypt depth significantly increased in the 16 mg/kg VA group (Quadratic, P = 0.015). The maltase (P = 0.032), sucrose (P = 0.041) and alkaline phosphatase activity (Linear, P = 0.024) were significantly increased when further supplemented with 4 mg/kg VA. Slc2a2 mRNA abundance was significantly increased in the 2 mg/kg VA group (Linear, P = 0.024). Moreover, the budding rates, buddings number per organoid and Chromogranin A and Muc2 expression of piglet intestinal organoids were significantly reduced (P < 0.05) by VA and its metabolites (retinoic acid). Compared with the control group, the expression of Spp1 and Trop2 increased. These results indicated that VA may increase the stemness of intestinal stem cell in vitro. This study suggested that VA could affect growth performance and intestinal function by regulating intestinal stem cells in the jejunum of weaned piglets.

语种： 英文

作者： 纪昌正;田俊杰;朱哲坤;王倩;孟禹璇;...

期刊： 中国畜牧杂志,2020年56(12):114-118 ISSN：0258-7033

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉 430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉 430023

关键词： 复合氨基酸;断奶仔猪;背最长肌

摘要： 本试验旨在研究复合氨基酸对断奶仔猪背最长肌生长的影响。选取16头体重（5.49±0.35）kg、健康的24日龄杜×长×大断奶仔猪随机分成对照组和复合氨基酸组,每组8个重复,每个重复1头猪。对照组饲喂基础日粮+0.99%的丙氨酸（等氮控制）,复合氨基酸组饲喂基础日粮+1.0%的复合氨基酸（谷氨酸:谷氨酰胺:甘氨酸:精氨酸:N-乙酰半胱氨酸=5:2:2:1:0.5）。试验期为21 d,于试验第21天麻醉、屠宰取样。结果表明:与对照组相比,复合氨基酸组提高了仔猪背最长肌蛋白质含量和蛋白质/DNA（P<0.05）,降低了RNA/DNA（P<0.05）;提高了背最长肌缬氨酸、组氨酸、3-甲基组氨酸和精氨酸的含量（P<0.05）,降低了背最长肌苏氨酸、谷氨酰胺、β-丙氨酸、赖氨酸和氧化型谷胱甘肽（GSSG）的含量（P<0.05）;提高了背最长肌PepT1和b0,+AT基因的相对表达量（P<0.05）,降低了MSTN基因的相对表达量（P<0.05）。结果表明添加复合氨基酸对断奶仔猪背最长肌的生长有一定的促进作用。

语种： 中文

作者： 赵广宇;孟禹璇;王倩;田俊杰;朱哲坤;...

期刊： 黑龙江畜牧兽医,2020年(16):95-99+104 ISSN：1004-7034

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉420023

关键词： 肉桂油;断奶仔猪;肝脏;激素;抗氧化;基因

摘要： 为了研究肉桂油对断奶仔猪肝脏的激素水平、抗氧化指标和相关基因相对表达量的影响,试验选取16头仔猪(杜×长×大)随机分为对照组和肉桂油组,每组8头,对照组饲喂基础日粮,肉桂油组在基础日粮中添加50 mg/kg的肉桂油,于试验第21天屠宰取样,测定和分析仔猪肝脏部分激素[类胰岛素生长因子1(IGF-1)、前列腺素E2(PGE2)]、炎性细胞因子[白介素1β(IL-13)]、抗氧化指标[谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、髓过氧化物酶(MPO)和总超氧化物歧化酶(T-SOD)]的含量和mTOR、EGFR信号通路相关基因[哺乳动物雷帕霉素靶蛋白(mTOR)、起始因子4E结合蛋白(4E-BP1)、70 ku核糖体蛋白S6激酶(P70S6K)、细胞外信号调节激酶(ERK)、丝氨酸/苏氨酸蛋白激酶(Akt)]、炎性和免疫相关基因[趋化因子9(CXCL9)、趋化因子11(CXCL11)、热休克105/110蛋白1(HSPH1)、干扰素α(IFN-α)、鼠黏液/流感病毒抵抗基因1(MX1)、鼠黏液/流感病毒抵抗基因2(MX2)、再生胰岛衍生蛋白3γ (REG3G)]及细胞凋亡相关基因[促凋亡基因(Bax)、低氧诱导因子-1(HIF-1)]的mRNA表达水平.结果 表明:与对照组比较,肉桂油组显著提高了断奶仔猪肝脏中IL-1β水平(P＜0.05),显著降低了肝脏PGE2水平(P＜0.05);显著提高了仔猪肝脏GSH-Px活性(P＜0.05),显著降低了MDA含量(P＜0.05);显著上调了仔猪肝脏mTOR、4 E-BP1、ERK、Bax、CXCL9、MX1、REG3G基因的mRNA相对表达量(P＜0.05),显著下调了Akt、CXCL11、HSPH1、IFN-α、MX2和HIF-1基因的mRNA相对表达量(P＜0.05).说明日粮中添加肉桂油可以调节仔猪肝脏PGE2和IL-1β水平,提高肝脏抗氧化能力,对断奶仔猪肝脏相关基因相对表达量有一定的调控作用.

语种： 中文

作者： 王蕾;易丹;吴涛;侯永清

期刊： 中国畜牧杂志,2019年55(8):6-12 ISSN：0258-7033

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,湖北武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉 430023;[侯永清; 王蕾; 易丹; 吴涛] 武汉轻工大学

关键词： 仔猪;肠道损伤;模型

摘要： 肠道不仅是所有营养物质消化吸收的最终场所,也是动物体内最大的免疫器官,是机体防御体系的第一道屏障,维护动物肠道健康对保障养猪生产至关重要。肠道是机体应激反应的中心器官,各种应激因素致使肠道结构损伤与功能紊乱,从而导致仔猪健康不良与生长受阻。因此,建立稳定可靠的肠道损伤模型是研究仔猪肠道损伤机制及营养干预的关键,本文主要介绍了本实验室成功建立的几种仔猪肠道损伤模型,筛选了几种肠道功能紊乱的标志性分子,为猪和人类的肠道功能障碍研究及营养干预提供参考。

语种： 中文

作者： 李雪妮;史宇涛;杜林晓;吕阳;马志鹏;...

期刊： 中国畜牧兽医,2019年46(4):1151-1157 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉 430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心 ,武汉430023;[杜林晓; 侯永清; 吕阳; 王蕾; 马志鹏; 易丹; 李雪妮; 赵迪; 史宇涛; 吴涛] 武汉轻工大学

关键词： 耐热肠毒素(STa);重组大肠杆菌;仔猪;炎症

摘要： 本试验旨在研究表达Ⅰ型耐热肠毒素(STa)的重组大肠杆菌诱发7日龄仔猪小肠炎症反应.选取24头7日龄健康仔猪,随机分为4个处理组:对照组(人工乳)、STa组(人工乳+2×109 CFU重组菌LMG194-pBAD-STa)、LMG194组(人工乳+2×109 CFU大肠杆菌LMG194)和K88组(人工乳+2×109 CFU大肠杆菌K88),每组6头.试验第5天进行攻毒,第7天屠宰取样,观察空肠组织形态学变化,并检测血清中炎性细胞因子含量及空肠免疫相关基因的相对表达量.结果显示,与对照组相比,S T a与K88组仔猪空肠绒毛明显萎缩变短,有明显损伤甚至脱落;STa、LMG194及K88组血清中IL-10、IL-8和IL-6浓度均显著升高(P<0.05),LMG194和K88组血液和肠道iFABP浓度均显著降低(P<0.05),STa和K88组血清中TNF-α浓度显著降低、NF-κB浓度显著升高,LMG194组NF-κB浓度显著降低、TNF-α浓度显著升高(P<0.05);STa和LMG194组CCL2和CXCL9基因表达水平显著上调(P<0.05),V N N1基因表达水平显著下调(P<0.05),STa和LMG194组IFN-γ组基因水平分别显著上升和下降(P<0.05);K88组CXCL9和IFN-γ基因表达水平显著下调(P<0.05),VNN1基因表达水平显著上调(P>0.05),CCL2基因表达水平无明显差异(P>0.05).以上结果表明,表达Ⅰ型耐热肠毒素STa的重组大肠杆菌几乎具有与K 88一样的毒性,可导致7日龄仔猪小肠黏膜受损,诱发严重的炎症反应,导致仔猪腹泻,可作为仔猪腹泻模型的候选菌株.

语种： 中文

作者： 宋转;赵广宇;董毅;纪昌正;李思源;...

期刊： 中国畜牧兽医,2019年(03):684-689 ISSN：1671-7236

作者机构： 1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室;2. 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心

关键词： 复合植物精油;断奶仔猪;脂多糖;肠黏膜;肠道形态结构;抗氧化能力

摘要： 为研究复合植物精油（OCT）对脂多糖（LPS）刺激断奶仔猪肠道结构和抗氧化能力的影响,本试验选取28日龄左右健康的杜×长×大三元杂交仔猪24头,按照体重相近原则随机分为3个处理组:对照组、LPS组和OCT组。对照组和LPS组仔猪饲喂基础日粮,OCT组仔猪饲喂基础日粮+50 mg/kg OCT。试验期21 d。于试验第21天,LPS组和OCT组仔猪注射LPS（100μg/（kg·BW））,对照组仔猪注射等量生理盐水。LPS或生理盐水注射后3 h采血;6 h后,屠宰全部仔猪取肠道样品测定有关指标。结果表明,与对照组相比,LPS组仔猪空肠绒毛高度/隐窝深度有降低趋势（P>0.05）,回肠绒毛高度及绒毛高度/隐窝深度显著降低（P<0.05）;血浆过氧化氢酶（CAT）活性、血浆和空肠超氧化物歧化酶（SOD）活性显著降低（P<0.05）,空肠诱导型一氧化氮合酶（iNOS）活性、血浆和回肠H2O2含量显著上升（P<0.05）。与LPS组相比,OCT组仔猪空肠绒毛高度、空肠绒毛高度/隐窝深度和回肠绒毛高度显著提高（P<0.05）,回肠绒毛高度/隐窝深度有提高趋势（P>0.05）;血浆CAT、空肠SOD活性显著提高（P<0.05）,血浆和回肠H2O2含量显著下降（P<0.05）。以上结果表明,日粮中添加50 mg/kg OCT可以在一定程度上缓解由LPS刺激引起的仔猪氧化应激,改善仔猪肠道结构。

语种： 中文

作者： 余魁;张越;李思源;纪昌正;董毅;...

期刊： 中国畜牧兽医,2019年46(5):1362-1369 ISSN：1671-7236

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;[赵广宇; 余魁; 董毅; 侯永清; 王蕾; 张越; 李思源; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;血浆抗氧化;肠黏膜形态;肠黏膜生长

摘要： 试验研究了日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响。选取30头体重相近的21日龄健康断奶仔猪随机分为3组(对照组、试验Ⅰ组和试验Ⅱ组),每组10个重复,每个重复1头猪。对照组饲喂基础日粮,试验Ⅰ、Ⅱ组在基础日粮的基础上分别添加2.0×10^6、2.0×10^7 CFU/g的凝结芽孢杆菌。正试期21 d,试验结束时前腔静脉采血后屠宰取样。结果显示:与对照组相比,试验Ⅰ和Ⅱ组断奶仔猪血浆超氧化物歧化酶(SOD)活性、空肠和回肠绒毛高度与隐窝深度的比值、回肠总蛋白(TP)含量及RNA与DNA的比值均显著提高(P<0.05);试验Ⅱ组仔猪血浆丙二醛(MDA)含量显著降低(P<0.05);试验Ⅰ组空肠TP含量及TP/DNA显著提高(P<0.05)。在本试验条件下,日粮中添加凝结芽孢杆菌可以提高断奶仔猪的抗氧化能力,促进仔猪肠道黏膜细胞的增殖与更新,从而改善了肠道黏膜形态结构。

语种： 中文

作者： 吴梦郡;纪昌正;李思源;董毅;赵广宇;...

期刊： 中国畜牧兽医,2019年46(3):669-676 ISSN：1671-7236

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉 430023;[赵广宇; 董毅; 侯永清; 吴梦郡; 王蕾; 张越; 李思源; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;小肠功能

摘要： 本研究旨在探究日粮中添加凝结芽孢杆菌（BC）对断奶仔猪小肠功能的影响。试验选取平均体重为（6.5±0.21） kg的21日龄三元杂交（杜×长×大）断奶仔猪24头,随机分为3组,每组8个重复,每个重复1头猪。对照组饲喂基础日粮,BCⅠ、BCⅡ组分别在基础日粮中添加2×106、2×107 CFU/g凝结芽孢杆菌。于试验第21天,所有仔猪灌服D-木糖（0.1 g/（kg·BW））,1 h后采血,屠宰,取回肠黏膜样品及回肠、结肠食糜。结果表明:与对照组相比,BCⅠ、BCⅡ组血浆二胺氧化酶（DAO）活力显著降低（P<0.05）,BCⅠ组血浆D-木糖含量显著增加（P<0.05）;BCⅠ、BCⅡ组回肠谷胱甘肽过氧化物酶（GSH-Px）的活力显著增加（P<0.05）,BCⅡ组回肠谷胱甘肽（GSH）含量增加（P>0.05）,BCⅠ组回肠丙二醛（MDA）含量显著降低（P<0.05）;BCⅠ组回肠黏膜水通道蛋白3（AQP3）、碱性氨基酸转运载体(b0,+AT)、钠葡萄糖共转运载体1（SGLT-1）的mRNA表达量均显著增加（P<0.05）,BCⅡ组回肠黏膜AQP3、AQP10、内向整流钾离子通道13（KCNJ13）、b0,+AT、SGLT-1的mRNA表达量均显著增加（P<0.05）;BCⅠ组回肠及结肠肠球菌、双歧杆菌数量均显著升高（P<0.05）,BCⅡ组回肠肠球菌及结肠肠球菌及双歧杆菌数量均显著升高（P<0.05）。综合试验结果,日粮中添加凝结芽孢杆菌可提高断奶仔猪小肠道吸收转运能力及抗氧化能力,改善断奶仔猪小肠内菌群结构。

语种： 中文

作者： Wang, Xiuying;Wang, Wenjun;Wang, Longmei;Yu, Cheng;Zhang, Guolong;...

期刊： FOOD & FUNCTION,2019年10(1):479-489 ISSN：2042-6496

通讯作者： Liu, Yulan

作者机构： [Zhang, Guolong; Zhao, Shengjun; Hu, Chien-An Andy; Liu, Yulan; Wang, Chunwei; Yu, Cheng; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Yu, Cheng; Wang, Wenjun] South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Hubei, Peoples R China.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

摘要： The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change. Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases. We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model. Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline). After feeding basal or LNT diet for 21 days, pigs were injected with LPS or saline. At 4 h post-injection, pigs were killed and jejunum, ileum and cecal digesta were collected. LNT improved intestinal morphology and barrier function. LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine. In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor. Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels. Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota. These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones. © 2019 The Royal Society of Chemistry.

语种： 英文

作者： Lv, Yang;Kang, Ping;Zhang, Lin;Li, Xueni;Wang, Lei;...

期刊： THAI JOURNAL OF VETERINARY MEDICINE,2018年48(2):147-156 ISSN：0125-6491

通讯作者： Wu, Tao

作者机构： [Wu, Tao; Lv, Yang; Kang, Ping; Hou, Yongqing; Wang, Lei; Li, Xueni; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.

通讯机构： [Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.

关键词： Epidermal growth factor;Intestinal histology;LPS-challenged mice;Lactobacillus acidophilus

摘要： The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named ΔMG6243-1 and ΔMG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 μl MG6243, 4) LPS-challenged + 200 μl ΔMG6243-1, 5) LPS-challenged + 200 μl ΔMG6243-1 + inducer IPTG, and 6) LPS-challenged + 200 μl ΔMG6243-2. There were ten replicates for each treatment, and each replicate had one mouse. On d 15, the LPS-challenged mice were injected intraperitoneally with LPS at 10 mg/kg BW, while the non-challenged mice were injected with 0.9% NaCl solution. Three hours after the challenge, all mice were killed. Results showed that both ΔMG6243-1 and ΔMG6243-2 could increase villus height in the duodenum, ileum and jejunum (P < 0.05). In addition, the recombinant L. acidophilus decreased the crypt depth in the duodenum and ileum (P < 0.05). These results indicated that the recombinant L. acidophilus could effectively improve the intestinal histology in LPS-challenged mice. © 2018 Chulalongkorn University Printing House.

语种： 英文

作者： Wu, Tao;Li, Kang;Yi, Dan;Wang, Lei;Zhao, Di;...

期刊： International Journal of Molecular Sciences,2018年19(10):3277- ISSN：1422-0067

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Li, Kang; Lv, Yang; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.

关键词： intestinal morphology and function;glycogen and fat metabolism;trihexanoin;weaned piglets

摘要： Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: The control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate; increased the concentrations of LDL, HDL and total protein in plasma; decreased cholesterol concentrations and glutamyl transpeptidase activity in plasma; improved intestinal morphologic structure; altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism, mucosal barrier function, antioxidant capacity and water transport capacity; and altered the community of intestinal microflora. These results indicate that dietary trihexanoin supplementation could reduce diarrhea, regulate carbohydrate and fat metabolism, exert beneficial effects on the intestinal mucosal barrier, protect the intestinal mucosa from injuries, improve intestinal transport and absorption, and enhance antioxidant capacity. In conclusion, dietary supplementation with 0.5% trihexanoin improves the intestinal function and health of weaned piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Wang, Weiwei;Chen, Juan;Zhou, Hang;Wang, Li;Ding, Sujuan;...

期刊： Food and Agricultural Immunology,2018年29(1):84-94 ISSN：0954-0105

通讯作者： Li, Aike

作者机构： [Wang, Yongwei; Wang, Li; Song, Dan; Wang, Weiwei; Ding, Sujuan; Li, Aike; Zhou, Hang; Chen, Juan] Acad State Adm Grain, 11 Baiwanzhuang St, Beijing 100037, Peoples R China.;[Li, Aike; Chen, Juan] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan, Hubei, Peoples R China.;[Ding, Sujuan] Hunan Agr Univ, Sch Biol Sci & Biotechnol, Changsha, Hunan, Peoples R China.

通讯机构： [Li, Aike] A;Acad State Adm Grain, 11 Baiwanzhuang St, Beijing 100037, Peoples R China.

关键词： Fructooligosaccharide;Immune;Lactobacillus plantarum;Microencapsulated;Weaned piglets

摘要： This experiment aimed to investigate the effects of a symbiotic, consisting of microencapsulated Lactobacillus plantarum (MLP) and fructooligosaccharide (FOS) on growth performance, blood immune parameters, and intestinal microbiota in weaned piglets. Ninety weaned piglets were assigned to three dietary groups (CON: basal diet; ANT: basal diet + aureomycin; SYN: basal diet + MLP and FOS) for a four-week trial. Compared to CON, pigs in the SYN group had higher weight gain and feed intake, and lower diarrhea rate (P < .05). Also, pigs in the SYN group had improved plasma IgA and IgG concentrations, and increased (P < .05) numbers of lactic acid bacteria in the colon compared to CON. In conclusion, feeding a synbiotic based on MLP and FOS had beneficial effects on growth performance, plasma immune parameters, and intestinal microbiota, indicating the potential of it to serve as an alternative to feed antibiotics in weaned pig diets. © 2017 The Author(s).

语种： 英文

作者： Yi, Dan;Liu, Wenkai;Hou, Yongqing*;Wang, Lei;Zhao, Di;...

期刊： Frontiers in Bioscience-Landmark,2018年23(11):2166-2176 ISSN：2768-6701

通讯作者： Hou, Yongqing

作者机构： [Liu, Wenkai; Wu, Tao; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Animal Model;Hematology;Indomethacin;Inflammation;Intestinal injury;Piglets;Review

摘要： A useful animal model of intestinal injury is pivotal for studying its pathogenesis and developing nutritional interventions (e.g., amino acid supplementation). Here, we propose the use of indomethacin (IDMT) to induce intestinal inflammation in neonatal pigs. Fourteen-day-old piglets fed a milk replacer diet receive intraperitoneal administration of IDMT (5 mg/kg body weight) for 3 consecutive days. On day 4, blood and intestinal samples are obtained for physiological and biochemical analyses. IDMT increases blood DAO activity, I-FABP concentration, neutrophil and eosinophil numbers; intestinal MMP3 mRNA levels, MPO activity, and MDA concentration; but reduces the plasma concentration of citrulline (synthesized exclusively by enterocytes of the small intestine), intestinal GSH-Px activity, and mRNA levels for villin, I-FABP, TRPV6, AQP10, and KCNJ13. Moreover, extensive hemorrhagic spots, thinned intestinal wall, and ulcers in the distal jejunum and ileum are observed in IDMT-challenged piglets. Furthermore, IDMT decreases intestinal villus height and villus surface area in the piglet jejunum. Collectively, this work establishes a porcine model of intestinal injury for designing novel nutritional means to improve gut function in pigs and humans. © 2018 Frontiers in Bioscience. All Rights Reserved.

语种： 英文

作者： 李鹏;吴梦郡;余魁;徐海旺;吴涛;...

期刊： 中国畜牧兽医,2018年45(6):1715-1721 ISSN：1671-7236

作者机构： [余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 易丹; 赵迪; 吴涛] 武汉轻工大学

关键词： 猪流行性腹泻病毒;仔猪;肠道损伤;模型

摘要： 试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组:对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为104.5 TCID50）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1g/kg体重）,1h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明:（1）PEDV感染显著降低了仔猪ADG（P<0.05）,极显著提高了仔猪腹泻率（P<0.01）;（2）PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01）,显著提高了十二指肠和空肠隐窝深度（P<0.05）;（3）PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01）,极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明,口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。

语种： 中文

作者： Yi, Dan;Li, Baocheng;Hou, Yongqing*;Wang, Lei;Zhao, Di;...

期刊： Amino Acids,2018年50(8):1089-1100 ISSN：0939-4451

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Li, Baocheng; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Zhou, Ying; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Amino acid blend;Intestinal function;Piglets

摘要： The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB. On day 20 of the trial, blood and intestinal tissue samples were obtained from piglets. Compared with the control, AAB supplementation reduced (P &lt; 0.05) diarrhoea incidence; plasma alanine aminotransferase and diamine oxidase activities; intestinal concentrations of hydrogen peroxide, malondialdehyde, and heat shock protein-70, and intestinal mRNA levels for interleukin-1β, interferon-γ, and chemokine (C–X–C motif) ligand-9; and the numbers of Enterobacterium family, Enterococcus genus and Clostridium coccoides in the colon digesta. Furthermore, AAB supplementation enhanced (P &lt; 0.05): the plasma concentrations of serine, aspartate, glutamate, cysteine, tyrosine, phenylalanine, tryptophan, lysine, arginine, citrulline, ornithine, taurine, and γ-aminobutyric acid; intestinal villus height and surface area, villus height/crypt depth ratio, antioxidative enzyme activities, and mRNA levels for porcine β-defensin-1, sodium-independent amino acid transporters (b 0,+ AT and y + LAT1), aquaporin (AQP) 3, AQP8, AQP10, nuclear factor erythroid 2-related factor 2 and glutathione S-transferase omega-2, and protein abundances of AQP3, AQP4, claudin-1, occludin and myxovirus resistance 1; and the numbers of Bifidobacterium genus and Lactobacillus genus in the colon digesta. Collectively, these comprehensive results indicate that dietary AAB supplementation plays an important role in improving piglet growth and intestinal function. © 2018, Springer-Verlag GmbH Austria, part of Springer Nature.

语种： 英文

作者： Wang, Longmei;Tu, Zhixiao;Wang, Haibo;Wang, Shuhui;Wang, Xiuying;...

期刊： Journal of Functional Foods,2018年46:482-489 ISSN：1756-4646

通讯作者： Liu, Yulan

作者机构： [Hu, Chien-An Andy; Liu, Yulan; Wang, Haibo; Wang, Shuhui; Tu, Zhixiao; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Flaxseed oil;Inflammation;Lipopolysaccharide;Liver;Necroptosis;Pigs

摘要： The hepatoprotective effects of flaxseed oil (FO) were tested in a pig model of lipopolysaccharide (LPS)-induced liver injury. Twenty-four piglets were used in a 2 × 2 factorial design including diet and an LPS challenge. After 3 weeks of feeding with 5% FO or 5% corn oil, pigs were challenged with LPS or saline. FO alleviated LPS-induced morphological liver damage, reduced serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities, and increased claudin-1 protein expression. FO decreased tumor necrosis factor-α interleukin-6 and cyclooxygenase 2 mRNA expression, and heat shock protein 70 mRNA and protein expression. FO downregulated receptor interacting protein kinase (RIP)1, RIP3, and mixed-lineage kinase domain-like protein (MLKL) mRNA expression, RIP1 and RIP3 protein expression, and MLKL phosphorylation. FO downregulated mRNA expression of toll-like receptor 4, nucleotide-binding oligomerization domain protein 1 and multiple downstream signaling molecules, and decreased p38 and extracellular signal-regulated kinase 1/2 phosphorylation. These results indicate that FO inhibits necroptotic and inflammatory signaling pathways to protect the liver from inflammation-related injury. © 2018 Elsevier Ltd

语种： 英文

作者： 张俊梅;宋转;周颖;刘文凯;易丹;...

期刊： 中国畜牧兽医,2018年45(9):2440-2446 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,武汉430023;[侯永清; 张俊梅; 王蕾; 周颖; 易丹; 宋转; 赵迪; 刘文凯] 武汉轻工大学

关键词： 三丁酸甘油酯;乙酸;仔猪;肠道屏障

摘要： 本试验旨在研究三丁酸甘油酯（TB）对乙酸（ACA）刺激仔猪小肠黏膜生长和肠道屏障功能的影响。试验选用18头体重接近、健康的28日龄断奶仔猪（杜×长×大）,随机分为3个处理:对照组、乙酸组和TB组,每个处理6个重复,每个重复1头猪。试验期为21d。试验期内对照组和乙酸组饲喂基础日粮,TB组饲喂基础日粮+0.1%TB。于试验第15天清晨,乙酸组和TB组仔猪直肠灌注10mL 10%乙酸,对照组直肠灌注相应剂量的灭菌生理盐水。于试验第18、21天清晨前腔静脉采血,测定血浆二胺氧化酶（DAO）活力;第21天屠宰取样,取空肠、回肠等组织样品,测定仔猪肠道形态结构、肠道黏膜损伤相关基因mRNA水平等指标。结果表明:（1）未用乙酸刺激前,与对照组相比,日粮中添加0.1%TB对仔猪平均日增重有一定程度提高（P=0.089）,且显著降低了仔猪料重比（P<0.05）;（2）与对照组相比,乙酸组显著降低了空肠黏膜绒毛高度、回肠黏膜绒毛高度与隐窝深度比值及空肠绒毛表面积（P<0.05）;（3）与乙酸组相比,TB组显著降低了血浆DAO活力（P<0.05）,极显著降低了回肠AREG基因的相对表达量（P<0.01）。综合上述结果,日粮中添加0.1%TB能缓解乙酸诱导的仔猪肠黏膜生长及肠道屏障功能的损伤。

语种： 中文

作者： 王惠云;廖满;王蕾;赵迪;易丹;...

期刊： 中国畜牧杂志,2018年54(2):52-59 ISSN：0258-7033

作者机构： 动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉 430023;[丁斌鹰; 廖满; 王惠云; 王蕾; 刘玉兰; 易丹; 赵迪] 武汉轻工大学

关键词： N-乙酰半胱氨酸;肉鸡;冷应激;肠道功能

摘要： 本试验旨在研究N-乙酰半胱氨酸(NAC)和冷应激对肉鸡肠道组织形态、能量状况和抗氧化能力的影响.试验将200只1日龄健康雄性科宝肉鸡随机分为4个处理组:对照组(基础日粮)、NAC组(基础日粮+0.1％NAC)、冷应激组(基础日粮)与冷应激+NAC组(基础日粮+0.1％NAC),每组5个重复,每个重复10只鸡.试验第1～2周按常规饲养方法控制温度,试验第3～6周冷应激组和冷应激+NAC组的温度控制在(10±2)℃,而对照组与NAC组维持在(26±2)℃.结果表明:冷应激处理使肉鸡平均日采食量、平均日增重、空肠低氧诱导因子1 (HIF-1) mRNA水平显著下降(P＜0.05),饲料转化率、回肠一磷酸腺苷(AMP)含量和空肠隐窝深度极显著升高(P＜0.01);日粮添加0.1％ NAC显著提高了冷应激肉鸡肠道绒毛高度(VH)、十二指肠与空肠绒毛高度与隐窝深度比值(VH/CD)、空肠三磷酸腺苷(ATP)和腺苷酸池(TAN)含量、回肠ATP和二磷酸腺苷(ADP)及能荷(AEC)含量、十二指肠超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力、回肠谷胱甘肽过氧化物酶(GSH-Px)活力、空肠血红素加氧酶(HMOX) mRNA水平(P＜0.05).因此,日粮中添加0.1％的N-乙酰半胱氨酸可改善肠道组织形态和能量状况,提高冷应激肉鸡肠道抗氧化能力.

语种： 中文

作者： 周佳;李鹏;程强;张俊梅;周颖;...

期刊： 中国畜牧杂志,2018年54(4):50-54 ISSN：0258-7033

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[李鹏; 周佳; 侯永清; 张俊梅; 程强; 王蕾; 周颖; 易丹] 武汉轻工大学

关键词： N-乙酰半胱氨酸;仔猪;游离氨基酸;小肠

摘要： 本试验旨在研究N-乙酰半胱氨酸(N-acetylcysteine,NAC)灌胃对超早期断奶仔猪血浆中游离氨基酸、小肠形态结构及功能的影响.将22头体重为(2.99±0.41) kg的7日龄断奶仔猪随机分为对照组和NAC组,每组1 1个重复,每个重复1头猪.在饲喂人工乳的基础上,NAC组每天每头按50 mg/kg体重进行NAC灌胃,对照组每天每头进行同体积的生理盐水灌胃.于试验第11天按0.1 g/kg体重的剂量给仔猪灌服D-木糖溶液,1h后前腔静脉采血,然后屠宰取小肠组织样,刮取肠黏膜及制作组织切片.结果表明:与对照组相比,NAC组显著提高了超早期断奶仔猪平均日增重(P＜0.05);提高了超早期断奶仔猪血浆中谷氨酰胺、胱氨酸、γ-氨基丁酸水平(P＜0.05),降低了血浆中尿素氮、半胱氨酸、色氨酸水平和二胺氧化酶活力(P＜0.05):NAC灌胃提高了哺乳仔猪十二指肠黏膜的绒毛高度/隐窝深度(P＜0.05),有降低十二指肠隐窝深度的趋势(P＜0.10),有提高空肠绒毛宽度和回肠绒毛高度的趋势(P＞0.10).在本试验条件下,NAC灌胃可改善小肠形态结构与屏障功能,提高氨基酸的利用率,调节血浆中部分氨基酸的含量.

语种： 中文

作者： 李鹏;汪中兴;吴梦郡;余魁;纪昌正;...

期刊： 中国畜牧杂志,2018年54(3):87-91 ISSN：0258-7033

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023

关键词： 复合植物精油;脂多糖;仔猪;肝脏

摘要： 试验旨在研究复合植物精油(OCT)对脂多糖(LPS)刺激仔猪肝脏的保护作用.选取18头仔猪,随机分为对照组、LPS组和OCT+LPS组,每组6个重复.对照组与LPS组饲喂基础日粮,OCT+LPS组在基础日粮中添加50 mg/kg OCT.试验期21 d.于试验第21天,LPS组与OCT+LPS组仔猪腹腔注射LPS,对照组注射等量的生理盐水,3h后采血,6h后屠宰.结果表明:与对照组相比,LPS刺激提高了血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷酰转肽酶(GGT)、肝脏诱导型一氧化氮合酶(i-NOS)的活力(P＜0.05),肝脏热休克蛋白70 (HSP70)基因的相对表达量显著提高(P＜0.05);仔猪肝脏表皮生长因子(EGF)、白细胞介素10 (IL-10)、胰岛素样因子(IGF-1)、丝氨酸蛋白激酶(mTOR)基因的相对表达量显著降低(P＜0.05).在日粮中添加50 mg/kg的OCT,可缓解LPS导致的仔猪血浆ALT、AST、GGT活力的升高以及肝脏i-NOS活力的升高(P＜0.05),且有缓解LPS导致的仔猪肝脏MPO活力升高的趋势(P＜0.1),另外可缓解LPS导致的仔猪肝脏HSP70基因相对表达量的升高以及EGF、IL-10、IGF-1、mTOR等基因相对表达量的降低(P＜0.05).综上可知,日粮中添加50 mg/kg的OCT可缓解LPS刺激引起的肝脏炎症反应,提高仔猪肝脏细胞的生长和增殖,进而缓解LPS刺激导致的仔猪肝脏氧化应激损伤.

语种： 中文