作者： Wu, Mengjun;Yi, Dan;Zhang, Qian;Wu, Tao;Yu, Kui;...

期刊： Scientific Reports,2021年11(1):6552 ISSN：2045-2322

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Peng, Meng; Yu, Kui; Hou, Yongqing; Wu, Mengjun; Zhang, Qian; Wang, Lei; Zhao, Di; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Mengjun] Univ Molise, Dept Agr Environm & Food Sci, I-86100 Campobasso, Italy.

通讯机构： [Hou, Yongqing] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.

摘要： <jats:title>Abstract</jats:title><jats:p>Puerarin has been reported to be an excellent antioxidant, anti-inflammatory and antimicrobial agent, but the potential effect of puerarin on porcine epidemic diarrhea virus (PEDV) is unclear. This study aimed to determine whether puerarin could alleviate intestinal injury in piglets infected with PEDV. A PEDV (Yunnan province strain) infection model was applied to 7-day-old piglets at 10<jats:sup>4.5</jats:sup> TCID<jats:sub>50</jats:sub> (50% tissue culture infectious dose). Piglets were orally administered with puerarin at the dosage of 0.5mg/kg body weight from day 5 to day 9. On day 9 of the trial, piglets were inoculated orally with PEDV. Three days later, jugular vein blood and intestinal samples were collected. Results showed puerarin reduced morbidity of piglets infected with PEDV. In addition, puerarin reduced the activities of aspartate aminotransferase and alkaline phosphatase, the ratio of serum aspartate aminotransferase to serum alanine aminotransferase, the number of white blood cells and neutrophils, and the plasma concentrations of interleukin-6, interleukin-8 and tumor necrosis factor-α, as well as protein abundances of heat shock protein-70 in PEDV-infected piglets. Moreover, puerarin increased D-xylose concentration but decreased intestinal fatty acid-binding protein concentration and diamine oxidase activity in the plasma of piglets infected with PEDV. Puerarin increased the activities of total superoxide dismutase, glutathione peroxidase and catalase, while decreasing the activities of myeloperoxidase and concentration of hydrogen peroxide in both the intestine and plasma of PEDV-infected piglets. Puerarin decreased mRNA levels of glutathione S-transferase omega 2 but increased the levels of nuclear factor erythroid 2-related factor 2<jats:italic>.</jats:italic> Furthermore, puerarin increased the abundance of total eubacteria (16S rRNA)<jats:italic>, Enterococcus</jats:italic> genus<jats:italic>, Lactobacillus</jats:italic> genus and <jats:italic>Enterobacteriaceae</jats:italic> family in the intestine, but reduced the abundance of <jats:italic>Clostridium coccoides</jats:italic> in the caecum. These data indicate puerarin improved intestinal function in piglets infected by PEDV and may be a promising supplement for the prevention of PEDV infection.</jats:p>

语种： 英文

作者： 董毅;王倩;孟禹璇;朱哲坤;田俊杰;...

期刊： 中国畜牧兽医,2020年47(8):2445-2453 ISSN：1671-7236

作者机构： [董毅; 侯永清; 王蕾; 田俊杰; 孟禹璇; 朱哲坤; 王倩; 赵迪] 武汉轻工大学

关键词： 复合氨基酸;断奶仔猪;肝脏;能量状态;游离氨基酸;基因

摘要： 为进一步探明复合功能性氨基酸的应用效果与机理,在前期研究的基础上,本试验研究了日粮中添加复合氨基酸对断奶仔猪肝脏能量状态、游离氨基酸及相关基因表达的影响.将16头体重相近的(21±3)日龄断奶仔猪随机分为对照组和复合氨基酸组,每组8个重复.分别饲喂添加0.99％丙氨酸(与试验组等氮)和1.00％复合氨基酸(谷氨酸∶谷氨酰胺∶精氨酸∶甘氨酸∶N-乙酰半胱氨酸=5∶2∶2∶1∶0.5)的基础日粮.于试验第21天前腔静脉采血,屠宰取肝脏样品,待测.结果 表明,与对照组相比,复合氨基酸显著降低了仔猪血浆ALT和AST活性(P＜0.05);显著提高了仔猪肝脏总蛋白含量、RNA/DNA和总蛋白/DNA(P＜0.05),显著降低了仔猪肝脏MDA和H2O2含量(P＜0.05);显著提高了肝脏精氨酸和二磷酸腺苷(ADP)含量(P＜0.05);显著上调了肝脏氨基酸转运载体b0,+ AT和y+LAT2基因、谷胱甘肽硫转移酶m2基因(GSTO2)、干扰素γ基因(IFN-γ)、脂蛋白酯酶基因(LPL)的表达(P＜0.05),显著下调了脂肪酸合成酶基因(FASN)的表达(P＜0.05).以上结果表明,日粮中添加1.00％复合氨基酸可以改善肝功能,提高抗氧化能力,可以调控仔猪肝脏能量状态及游离氨基酸代谢,并可能影响肝脏的脂肪代谢和免疫功能.

语种： 中文

作者： Wu, Tao;Li, Kang;Lyu, Yang;Yi, Dan;Zhao, Di;...

期刊： FRONTIERS IN BIOSCIENCE-LANDMARK,2020年25(7):1324-1336 ISSN：2768-6701

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Li, Kang; Lyu, Yang; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Engn Res Ctr Feed Prot Resources Agr By Prod, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.

关键词： Intestinal function;Lipid metabolism;Trilactic glyceride;Weaned piglets, Review

摘要： Glycerol-lactate esters are energy supplements for exercise, but effects of trilactic glyceride (TLG) on intestinal function and hepatic metabolism are unknown. We found that dietary supplementation with 0.5% TLG to weanling piglets decreased plasma concentrations of low-density lipoprotein and gamma-glutamyl transferase but increased those of D-xylose and high-density lipoprotein. TLG supplementation enhanced mRNA levels for fatty acid synthase (FASN) and SLC27A2 in white adipose tissue; insulin receptor in duodenum; aquaporin-8 in ileum, jejunum and colon; aquaporin-10 in duodenum and ileum; nuclear factor like-2 in jejunum and colon; glutathione S-transferase and phosphoenolpyruvate carboxykinase-1 in intestines; and abundances of claudin-1 and occludin proteins. TLG supplementation decreased mRNA levels for: hepatic hormone-sensitive lipase E, lipoprotein lipase, FASN, insulin-like growth factor-binding protein-3, and SLC27A2; and intestinal lipoprotein lipase, FASN and NADPH oxidase. Furthermore, TLG supplementation enhanced abundances of genus Bifidobacterium, while reducing abundances of family Enterobacteriaceae in ileum, colon and cecum; jejunal caspase-3 protein and diarrhea rate. In conclusion, dietary supplementation with TLG modulated lipid metabolism and alleviated diarrhea by improving intestinal function and regulating intestinal microflora in piglets. © 2020 Frontiers in Bioscience. All rights reserved.

语种： 英文

作者： 彭梦;王惠云;李诚诚;赵迪;王蕾;...

期刊： 饲料工业,2020年41(3):26-30 ISSN：1001-991X

作者机构： 动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院,湖北武汉430023;[李诚诚; 丁斌鹰; 王惠云; 彭梦; 王蕾; 易丹; 赵迪; 吴涛] 动物营养与饲料科学湖北省重点实验室

关键词： 谷氨酰胺;仔猪;肝脏;大豆分离蛋白;抗氧化功能

摘要： 试验旨在研究谷氨酰胺(Gln)对灌服大豆分离蛋白(SPI)仔猪肝脏和肌肉抗氧化功能及肝脏形态结构的影响.将24头7日龄健康仔猪(杜×长×大)随机分为3组:对照组、SPI组、SPI+Gln组,每组8个重复,每个重复1头猪.试验第1～5 d各组仔猪均饲喂人工乳,试验第6～7 d SPI组和SPI+Gln组每头仔猪饲喂含大豆分离蛋白(10 g/kg BW)的人工乳,对照组饲喂含酪蛋白(7.8 g/kg BW)的人工乳.试验第8～10 d SPI + Gln组灌服Gln(250 mg/kg BW),其余2组灌服丙氨酸(305 mg/kg BW).试验第11 d屠宰仔猪,取肝脏、腓肠肌、背最长肌组织样品,检测抗氧化酶活性和氧化产物的含量,并制作苏木素-伊红染色肝脏切片.结果表明,SPI可诱导肝脏脂肪变性、细胞空泡化和细胞排列无序,并提高了仔猪肝脏MDA(P＜0.05)、腓肠肌H2O2及背最长肌MDA的含量,但显著降低了腓肠肌、背最长肌CAT和GSH-Px活性.Gln处理缓解了SPI仔猪肝细胞形态的异常与脂肪变性,提高了肝脏GSH-Px活性,并显著降低了SPI仔猪肝脏MDA和腓肠肌的H2O2的含量(P＜0.05).因此,SPI诱导仔猪肝脏形态结构异常、肝脏和肌肉抗氧化能力下降,而Gln可有效缓解肝细胞损伤,提高仔猪肝脏与腓肠肌抗氧化能力.

语种： 中文

作者： 李雪妮;史宇涛;杜林晓;吕阳;马志鹏;...

期刊： 中国畜牧兽医,2019年46(4):1151-1157 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室 ,武汉 430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心 ,武汉430023;[杜林晓; 侯永清; 吕阳; 王蕾; 马志鹏; 易丹; 李雪妮; 赵迪; 史宇涛; 吴涛] 武汉轻工大学

关键词： 耐热肠毒素(STa);重组大肠杆菌;仔猪;炎症

摘要： 本试验旨在研究表达Ⅰ型耐热肠毒素(STa)的重组大肠杆菌诱发7日龄仔猪小肠炎症反应.选取24头7日龄健康仔猪,随机分为4个处理组:对照组(人工乳)、STa组(人工乳+2×109 CFU重组菌LMG194-pBAD-STa)、LMG194组(人工乳+2×109 CFU大肠杆菌LMG194)和K88组(人工乳+2×109 CFU大肠杆菌K88),每组6头.试验第5天进行攻毒,第7天屠宰取样,观察空肠组织形态学变化,并检测血清中炎性细胞因子含量及空肠免疫相关基因的相对表达量.结果显示,与对照组相比,S T a与K88组仔猪空肠绒毛明显萎缩变短,有明显损伤甚至脱落;STa、LMG194及K88组血清中IL-10、IL-8和IL-6浓度均显著升高(P<0.05),LMG194和K88组血液和肠道iFABP浓度均显著降低(P<0.05),STa和K88组血清中TNF-α浓度显著降低、NF-κB浓度显著升高,LMG194组NF-κB浓度显著降低、TNF-α浓度显著升高(P<0.05);STa和LMG194组CCL2和CXCL9基因表达水平显著上调(P<0.05),V N N1基因表达水平显著下调(P<0.05),STa和LMG194组IFN-γ组基因水平分别显著上升和下降(P<0.05);K88组CXCL9和IFN-γ基因表达水平显著下调(P<0.05),VNN1基因表达水平显著上调(P>0.05),CCL2基因表达水平无明显差异(P>0.05).以上结果表明,表达Ⅰ型耐热肠毒素STa的重组大肠杆菌几乎具有与K 88一样的毒性,可导致7日龄仔猪小肠黏膜受损,诱发严重的炎症反应,导致仔猪腹泻,可作为仔猪腹泻模型的候选菌株.

语种： 中文

作者： 刘文凯;董毅;史宇涛;纪昌正;李思源;...

期刊： 黑龙江畜牧兽医,2019年(24):97-100+105 ISSN：1004-7034

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉 430023;[赵广宇; 董毅; 侯永清; 王蕾; 李思源; 易丹; 赵迪; 史宇涛; 纪昌正; 刘文凯] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;肠道;抗氧化;抗病毒

摘要： 为了研究日粮中添加凝结芽抱杆菌对断奶仔猪肠道抗氧化和抗病毒能力的影响,试验选取24头 21 日龄的健康断奶仔猪(杜×长×大),随机分为 3组(对照组、BC6 组、BC7 组),每组 8个重复,对照组饲喂基础日粮,BC6组饲喂基础日粮+凝结芽抱杆菌(2×106 cfu/g饲料),BC7组饲喂基础日粮+凝结芽抱杆菌(2×107 cfu/g饲料),于试验第 21天屠宰取样,检测空肠都膜组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力和丙二酶(MDA)、过氧化氢(H202)含量,以及空肠炎性抗病毒和免疫调节相关基因[热休龙蛋白 1(HSPH1)、趋化因子(CCL2)、干扰素-α(IFN-α)、干扰素-?(IFN-?)、白细胞介素-4(IL-4)、抗氧化应激转录因子 2(Nrf-2)基因]及相关蛋白 [2'-5'寡聚腺普酸合成酶 1(OAS1)、抗黏液病毒 1(MX1)]的相对表达量.结果表明:与对照组相比,BC6组空肠MDA、H202的含量显著降低(P＜0.05),HSPH1.,IFN-?基因的相对表达量显著下调(P＜0.05),0ASl蛋白相对表达量显著升高(P＜0.05);BC7组空肠 H202的含量显著降低(P＜0.05),SOD和 CAT活力显著升高(P＜0.05),IL-4、HSPHl和 Nrf-2基因的相对表达量显著下调(P＜0.05),IFN-?基因的相对表达量显著上调(P＜0.05),MX1蛋白相对表达量显著升高(P＜0.05).说明日粮中添加凝结芽抱杆菌可以提高断奶仔猪肠道抗氧化能力和抗病毒能力,促进仔猪肠道吸收,从而维护肠道稳态,且日粮中添加 2×107cfu/g的凝结芽抱杆菌效果更好.

语种： 中文

作者： 余魁;张越;李思源;纪昌正;董毅;...

期刊： 中国畜牧兽医,2019年46(5):1362-1369 ISSN：1671-7236

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;[赵广宇; 余魁; 董毅; 侯永清; 王蕾; 张越; 李思源; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;血浆抗氧化;肠黏膜形态;肠黏膜生长

摘要： 试验研究了日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响。选取30头体重相近的21日龄健康断奶仔猪随机分为3组(对照组、试验Ⅰ组和试验Ⅱ组),每组10个重复,每个重复1头猪。对照组饲喂基础日粮,试验Ⅰ、Ⅱ组在基础日粮的基础上分别添加2.0×10^6、2.0×10^7 CFU/g的凝结芽孢杆菌。正试期21 d,试验结束时前腔静脉采血后屠宰取样。结果显示:与对照组相比,试验Ⅰ和Ⅱ组断奶仔猪血浆超氧化物歧化酶(SOD)活性、空肠和回肠绒毛高度与隐窝深度的比值、回肠总蛋白(TP)含量及RNA与DNA的比值均显著提高(P<0.05);试验Ⅱ组仔猪血浆丙二醛(MDA)含量显著降低(P<0.05);试验Ⅰ组空肠TP含量及TP/DNA显著提高(P<0.05)。在本试验条件下,日粮中添加凝结芽孢杆菌可以提高断奶仔猪的抗氧化能力,促进仔猪肠道黏膜细胞的增殖与更新,从而改善了肠道黏膜形态结构。

语种： 中文

作者： Zhang, Junmei;Zhao, Di;Yi, Dan;Wu, Mengjun;Chen, Hongbo;...

期刊： Scientific Reports,2019年9(1):19798 ISSN：2045-2322

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Zhou, Jia; Hou, Yongqing; Wu, Mengjun; Li, Peng; Zhao, Di; Zhang, Junmei; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

摘要： Porcine epidemic diarrhea virus (PEDV) infection can induce intestinal dysfunction, resulting in severe diarrhea and even death, but the mode of action underlying these viral effects remains unclear. This study determined the effects of PEDV infection on intestinal absorption and the expression of genes for nutrient transporters via biochemical tests and microarray analysis. Sixteen 7-day-old healthy piglets fed a milk replacer were randomly allocated to one of two groups. After 5-day adaption, piglets (n = 8/group) were orally administrated with either sterile saline or PEDV (the strain from Yunnan province) at 10(4.5) TCID50 (50% tissue culture infectious dose) per pig. All pigs were orally infused D-xylose (0.1 g/kg BW) on day 5 post PEDV or saline administration. One hour later, jugular vein blood samples as well as intestinal samples were collected for further analysis. In comparison with the control group, PEDV infection increased diarrhea incidence, blood diamine oxidase activity, and iFABP level, while reducing growth and plasma D-xylose concentration in piglets. Moreover, PEDV infection altered plasma and jejunal amino acid profiles, and decreased the expression of aquaporins and amino acid transporters (L-type amino acid transporter 1, sodium-independent amino acid transporter, B( degrees (,+))-type amino acid transport protein, sodium-dependent neutral amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 3, and peptide transporter (1), lipid transport and metabolism-related genes (lipoprotein lipase, apolipoprotein A1, apolipoprotein A4, apolipoprotein C2, solute carrier family 27 member 2, solute carrier family 27 member 4, fatty acid synthase, and long-chain acyl-CoA synthetase (3), and glucose transport genes (glucose transporter-2 and insulin receptor) in the jejunum. However, PEDV administration increased mRNA levels for phosphoenolpyruvate carboxykinase 1, argininosuccinate synthase 1, sodium/glucose co-transporter-1, and cystic fibrosis transmembrane conductance regulator in the jejunum. Collectively, these comprehensive results indicate that PEDV infection induces intestinal injury and inhibits the expression of genes encoding for nutrient transporters.

语种： 英文

作者： 吴梦郡;纪昌正;李思源;董毅;赵广宇;...

期刊： 中国畜牧兽医,2019年46(3):669-676 ISSN：1671-7236

作者机构： 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉,430023;武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,武汉 430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉 430023;[赵广宇; 董毅; 侯永清; 吴梦郡; 王蕾; 张越; 李思源; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;小肠功能

摘要： 本研究旨在探究日粮中添加凝结芽孢杆菌（BC）对断奶仔猪小肠功能的影响。试验选取平均体重为（6.5±0.21） kg的21日龄三元杂交（杜×长×大）断奶仔猪24头,随机分为3组,每组8个重复,每个重复1头猪。对照组饲喂基础日粮,BCⅠ、BCⅡ组分别在基础日粮中添加2×106、2×107 CFU/g凝结芽孢杆菌。于试验第21天,所有仔猪灌服D-木糖（0.1 g/（kg·BW））,1 h后采血,屠宰,取回肠黏膜样品及回肠、结肠食糜。结果表明:与对照组相比,BCⅠ、BCⅡ组血浆二胺氧化酶（DAO）活力显著降低（P<0.05）,BCⅠ组血浆D-木糖含量显著增加（P<0.05）;BCⅠ、BCⅡ组回肠谷胱甘肽过氧化物酶（GSH-Px）的活力显著增加（P<0.05）,BCⅡ组回肠谷胱甘肽（GSH）含量增加（P>0.05）,BCⅠ组回肠丙二醛（MDA）含量显著降低（P<0.05）;BCⅠ组回肠黏膜水通道蛋白3（AQP3）、碱性氨基酸转运载体(b0,+AT)、钠葡萄糖共转运载体1（SGLT-1）的mRNA表达量均显著增加（P<0.05）,BCⅡ组回肠黏膜AQP3、AQP10、内向整流钾离子通道13（KCNJ13）、b0,+AT、SGLT-1的mRNA表达量均显著增加（P<0.05）;BCⅠ组回肠及结肠肠球菌、双歧杆菌数量均显著升高（P<0.05）,BCⅡ组回肠肠球菌及结肠肠球菌及双歧杆菌数量均显著升高（P<0.05）。综合试验结果,日粮中添加凝结芽孢杆菌可提高断奶仔猪小肠道吸收转运能力及抗氧化能力,改善断奶仔猪小肠内菌群结构。

语种： 中文

作者： 吴梦郡;李思源;纪昌正;余魁;董毅;...

期刊： 中国畜牧兽医,2019年46(2):424-432 ISSN：1671-7236

作者机构： [赵广宇; 余魁; 董毅; 侯永清; 吴梦郡; 李思源; 赵迪; 吴涛; 纪昌正] 武汉轻工大学

关键词： 耐热肠毒素(STa);重组大肠杆菌;仔猪;肠道

摘要： 试验旨在研究表达Ⅰ型耐热肠毒素(S T a)的重组大肠杆菌对7日龄仔猪小肠功能的影响.选取24头7日龄仔猪,随机分为4个处理组,分别为对照组、STa组、LMG194组和K88组.整个试验期间均以人工乳饲喂,预饲期4 d,试验第5天进行攻毒,STa组、LMG194组及K88组分别在早晚两次口服灌喂2×109 CFU大肠杆菌LMG194-pBAD-STa、LMG194和K88,对照组灌服等量生理盐水,试验第7天早上各组灌服D-木糖并采血,屠宰并取空肠、回肠组织样品,测量仔猪血常规指标、血浆D-木糖含量及二胺氧化酶(DAO)活力,以及吸收与转运相关基因与蛋白表达量.结果显示,与对照组相比,STa组仔猪血液中中性粒细胞、中性粒细胞比率显著降低(P<0.05),淋巴细胞比率显著升高(P<0.05),LMG194组与K88组仔猪血液中中性粒细胞比率显著降低(P<0.05),淋巴细胞比率显著升高(P<0.05);STa组与K88组仔猪血浆D-木糖含量显著降低(P<0.05);STa组、LMG194组及K88组血浆DAO活力显著升高(P<0.05);STa组与LMG194组空肠AQP8、AQP10、KCN J13和b0,+A T基因表达量均显著降低(P<0.05),K88组空肠AQP8、AQP10基因表达量显著升高(P<0.05);STa组回肠AQP8基因表达量显著降低(P<0.05),AQP10、SGLT-1基因表达量显著升高(P<0.05),LMG194组、K88组AQP8基因表达量显著升高(P<0.05),K88组AQP10、KCNJ13、b0,+AT、SGLT-1基因表达量显著降低(P<0.05);STa组、LMG194组及K88组空肠HSP70蛋白的表达量显著升高(P<0.05).以上结果表明,表达Ⅰ型耐热肠毒素的重组大肠杆菌可导致7日龄仔猪小肠产生炎症,吸收与转运能力下降.

语种： 中文

作者： Wu, Tao;Li, Kang;Yi, Dan;Wang, Lei;Zhao, Di;...

期刊： International Journal of Molecular Sciences,2018年19(10):3277- ISSN：1661-6596

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Li, Kang; Lv, Yang; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr Byprod, Hubei Key Lab Anim Nutr & Feed Sci, Minist Educ, Wuhan 430023, Hubei, Peoples R China.

关键词： intestinal morphology and function;glycogen and fat metabolism;trihexanoin;weaned piglets

摘要： Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: The control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate; increased the concentrations of LDL, HDL and total protein in plasma; decreased cholesterol concentrations and glutamyl transpeptidase activity in plasma; improved intestinal morphologic structure; altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism, mucosal barrier function, antioxidant capacity and water transport capacity; and altered the community of intestinal microflora. These results indicate that dietary trihexanoin supplementation could reduce diarrhea, regulate carbohydrate and fat metabolism, exert beneficial effects on the intestinal mucosal barrier, protect the intestinal mucosa from injuries, improve intestinal transport and absorption, and enhance antioxidant capacity. In conclusion, dietary supplementation with 0.5% trihexanoin improves the intestinal function and health of weaned piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Lv, Yang;Kang, Ping;Zhang, Lin;Li, Xueni;Wang, Lei;...

期刊： THAI JOURNAL OF VETERINARY MEDICINE,2018年48(2):147-156 ISSN：0125-6491

通讯作者： Wu, Tao

作者机构： [Wu, Tao; Lv, Yang; Kang, Ping; Hou, Yongqing; Wang, Lei; Li, Xueni; Zhao, Di; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.

通讯机构： [Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.

关键词： Epidermal growth factor;Intestinal histology;LPS-challenged mice;Lactobacillus acidophilus

摘要： The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named ΔMG6243-1 and ΔMG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 μl MG6243, 4) LPS-challenged + 200 μl ΔMG6243-1, 5) LPS-challenged + 200 μl ΔMG6243-1 + inducer IPTG, and 6) LPS-challenged + 200 μl ΔMG6243-2. There were ten replicates for each treatment, and each replicate had one mouse. On d 15, the LPS-challenged mice were injected intraperitoneally with LPS at 10 mg/kg BW, while the non-challenged mice were injected with 0.9% NaCl solution. Three hours after the challenge, all mice were killed. Results showed that both ΔMG6243-1 and ΔMG6243-2 could increase villus height in the duodenum, ileum and jejunum (P < 0.05). In addition, the recombinant L. acidophilus decreased the crypt depth in the duodenum and ileum (P < 0.05). These results indicated that the recombinant L. acidophilus could effectively improve the intestinal histology in LPS-challenged mice. © 2018 Chulalongkorn University Printing House.

语种： 英文

作者： 李鹏;吴梦郡;余魁;徐海旺;吴涛;...

期刊： 中国畜牧兽医,2018年45(6):1715-1721 ISSN：1671-7236

作者机构： [余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 易丹; 赵迪; 吴涛] 武汉轻工大学

关键词： 猪流行性腹泻病毒;仔猪;肠道损伤;模型

摘要： 试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组:对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为104.5 TCID50）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1g/kg体重）,1h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明:（1）PEDV感染显著降低了仔猪ADG（P<0.05）,极显著提高了仔猪腹泻率（P<0.01）;（2）PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01）,显著提高了十二指肠和空肠隐窝深度（P<0.05）;（3）PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01）,极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明,口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。

语种： 中文

作者： Yi, Dan;Li, Baocheng;Hou, Yongqing*;Wang, Lei;Zhao, Di;...

期刊： Amino Acids,2018年50(8):1089-1100 ISSN：0939-4451

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Li, Baocheng; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Zhou, Ying; Ding, Binying; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Amino acid blend;Intestinal function;Piglets

摘要： The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB. On day 20 of the trial, blood and intestinal tissue samples were obtained from piglets. Compared with the control, AAB supplementation reduced (P &lt; 0.05) diarrhoea incidence; plasma alanine aminotransferase and diamine oxidase activities; intestinal concentrations of hydrogen peroxide, malondialdehyde, and heat shock protein-70, and intestinal mRNA levels for interleukin-1β, interferon-γ, and chemokine (C–X–C motif) ligand-9; and the numbers of Enterobacterium family, Enterococcus genus and Clostridium coccoides in the colon digesta. Furthermore, AAB supplementation enhanced (P &lt; 0.05): the plasma concentrations of serine, aspartate, glutamate, cysteine, tyrosine, phenylalanine, tryptophan, lysine, arginine, citrulline, ornithine, taurine, and γ-aminobutyric acid; intestinal villus height and surface area, villus height/crypt depth ratio, antioxidative enzyme activities, and mRNA levels for porcine β-defensin-1, sodium-independent amino acid transporters (b 0,+ AT and y + LAT1), aquaporin (AQP) 3, AQP8, AQP10, nuclear factor erythroid 2-related factor 2 and glutathione S-transferase omega-2, and protein abundances of AQP3, AQP4, claudin-1, occludin and myxovirus resistance 1; and the numbers of Bifidobacterium genus and Lactobacillus genus in the colon digesta. Collectively, these comprehensive results indicate that dietary AAB supplementation plays an important role in improving piglet growth and intestinal function. © 2018, Springer-Verlag GmbH Austria, part of Springer Nature.

语种： 英文

作者： Yi, Dan;Liu, Wenkai;Hou, Yongqing*;Wang, Lei;Zhao, Di;...

期刊： FRONTIERS IN BIOSCIENCE-LANDMARK,2018年23(11):2166-2176 ISSN：2768-6701

通讯作者： Hou, Yongqing

作者机构： [Liu, Wenkai; Wu, Tao; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Animal Model;Hematology;Indomethacin;Inflammation;Intestinal injury;Piglets;Review

摘要： A useful animal model of intestinal injury is pivotal for studying its pathogenesis and developing nutritional interventions (e.g., amino acid supplementation). Here, we propose the use of indomethacin (IDMT) to induce intestinal inflammation in neonatal pigs. Fourteen-day-old piglets fed a milk replacer diet receive intraperitoneal administration of IDMT (5 mg/kg body weight) for 3 consecutive days. On day 4, blood and intestinal samples are obtained for physiological and biochemical analyses. IDMT increases blood DAO activity, I-FABP concentration, neutrophil and eosinophil numbers; intestinal MMP3 mRNA levels, MPO activity, and MDA concentration; but reduces the plasma concentration of citrulline (synthesized exclusively by enterocytes of the small intestine), intestinal GSH-Px activity, and mRNA levels for villin, I-FABP, TRPV6, AQP10, and KCNJ13. Moreover, extensive hemorrhagic spots, thinned intestinal wall, and ulcers in the distal jejunum and ileum are observed in IDMT-challenged piglets. Furthermore, IDMT decreases intestinal villus height and villus surface area in the piglet jejunum. Collectively, this work establishes a porcine model of intestinal injury for designing novel nutritional means to improve gut function in pigs and humans. © 2018 Frontiers in Bioscience. All Rights Reserved.

语种： 英文

作者： 张俊梅;宋转;周颖;刘文凯;易丹;...

期刊： 中国畜牧兽医,2018年45(9):2440-2446 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,武汉430023;[侯永清; 张俊梅; 王蕾; 周颖; 易丹; 宋转; 赵迪; 刘文凯] 武汉轻工大学

关键词： 三丁酸甘油酯;乙酸;仔猪;肠道屏障

摘要： 本试验旨在研究三丁酸甘油酯（TB）对乙酸（ACA）刺激仔猪小肠黏膜生长和肠道屏障功能的影响。试验选用18头体重接近、健康的28日龄断奶仔猪（杜×长×大）,随机分为3个处理:对照组、乙酸组和TB组,每个处理6个重复,每个重复1头猪。试验期为21d。试验期内对照组和乙酸组饲喂基础日粮,TB组饲喂基础日粮+0.1%TB。于试验第15天清晨,乙酸组和TB组仔猪直肠灌注10mL 10%乙酸,对照组直肠灌注相应剂量的灭菌生理盐水。于试验第18、21天清晨前腔静脉采血,测定血浆二胺氧化酶（DAO）活力;第21天屠宰取样,取空肠、回肠等组织样品,测定仔猪肠道形态结构、肠道黏膜损伤相关基因mRNA水平等指标。结果表明:（1）未用乙酸刺激前,与对照组相比,日粮中添加0.1%TB对仔猪平均日增重有一定程度提高（P=0.089）,且显著降低了仔猪料重比（P<0.05）;（2）与对照组相比,乙酸组显著降低了空肠黏膜绒毛高度、回肠黏膜绒毛高度与隐窝深度比值及空肠绒毛表面积（P<0.05）;（3）与乙酸组相比,TB组显著降低了血浆DAO活力（P<0.05）,极显著降低了回肠AREG基因的相对表达量（P<0.01）。综合上述结果,日粮中添加0.1%TB能缓解乙酸诱导的仔猪肠黏膜生长及肠道屏障功能的损伤。

语种： 中文

作者： 张越;徐海旺;李鹏;周颖;张俊梅;...

期刊： 黑龙江畜牧兽医,2018年(12):165-168 ISSN：1004-7034

作者机构： 武汉轻工大学动物科学与营养工程学院/动物营养与饲料科学湖北省重点实验室,武汉,430023;[李鹏; 侯永清; 徐海旺; 张俊梅; 张越; 周颖; 赵迪] 武汉轻工大学

关键词： 凝结芽孢杆菌;断奶仔猪;生长性能;肠道菌群;血液生化指标

摘要： 为了研究日粮中添加凝结芽孢杆菌对仔猪生长性能、肠道菌群和血液生化指标的影响,试验选取21日龄健康断奶仔猪30头,随机分为3组(对照组、试验Ⅰ组、试验Ⅱ组),对照组饲喂基础日粮,试验Ⅰ组、试验Ⅱ组分别在基础日粮中添加凝结芽孢杆菌2.0× 106 cfu/g、2.0× 107 cfu/g.预试期3d,正试期21 d.于试验第21天采血,屠宰,测定其生长性能、肠道菌群和血液生化指标.结果表明:日粮中添加不同水平的凝结芽孢杆菌均显著降低了断奶仔猪的腹泻率(P＜0.05);提高了仔猪回肠内容物中乳酸菌的数量(P＜0.05),降低了回肠内容物中梭菌的数量(P＜0.05);提高了血浆中磷酸丝氨酸、胱氨酸、酪氨酸等多种氨基酸的含量(P＜0.05),降低了磷酰乙醇铵的含量(P＜0.05).说明在日粮中添加凝结芽孢杆菌可以改善断奶仔猪的肠道菌群,降低仔猪的腹泻率.

语种： 中文

作者： 周颖;宋转;张俊梅;刘文凯;余魁;...

期刊： 中国饲料,2018年(15):48-53 ISSN：1004-3314

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[余魁; 侯永清; 张俊梅; 吴梦郡; 王蕾; 周颖; 易丹; 宋转; 赵迪; 刘文凯] 武汉轻工大学

关键词： N-乙酰半胱氨酸;乙酸;仔猪;肠黏膜

摘要： 为研究N-乙酰半胱氨酸（NAC）对乙酸（ACA）刺激仔猪肠黏膜屏障功能以及肠道黏膜生长的影响,试验选取体重相似且体况健康的仔猪18头,随机分为对照组、ACA组和NAC组,其中前两组饲喂基础饲粮,NAC组在基础饲粮中添加0.05%NAC,每组6个重复,每个重复1头猪。试验第15天对ACA组和NAC组仔猪直肠灌注10 m L10%的乙酸,对照组灌注相应剂量的生理盐水。第21天灌服D-木糖溶液后采集前腔静脉血样,深度麻醉后屠宰,取小肠黏膜样品待测。结果表明:与对照组相比,ACA组仔猪血浆二胺氧化酶（DAO）活性、十二指肠和回肠的隐窝深度分别提高了94.80%、14.31%、8.14%（P<0.05）;十二指肠DAO活性降低了17.95%（P<0.05）,空肠绒毛高度、十二指肠和回肠绒毛高度/隐窝深度比值,十二指肠和空肠绒毛表面积,及回肠RNA/DNA比值和TP/DNA比值分别降低了14.03%、26.72%、14.97%、12.40%、37.11%、38.91%、26.35%（P<0.05）。饲粮中添加NAC可以缓解以上这些负面影响。此外,相较于ACA组,NAC血浆中D-木糖和表皮生长因子（EGF）含量以及回肠中EGF m RNA水平提高了17.29%、26.20%、34.03%（P<0.05）。综上所述,NAC能够改善ACA刺激造成的仔猪小肠黏膜屏障功能受损,NAC可能是通过提高EGF含量以及EGF m RNA水平,促进细胞生长,修复肠道损伤。

语种： 中文

作者： 王惠云;廖满;王蕾;赵迪;易丹;...

期刊： 中国畜牧杂志,2018年54(2):52-59 ISSN：0258-7033

作者机构： 动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉 430023;[丁斌鹰; 廖满; 王惠云; 王蕾; 刘玉兰; 易丹; 赵迪] 武汉轻工大学

关键词： N-乙酰半胱氨酸;肉鸡;冷应激;肠道功能

摘要： 本试验旨在研究N-乙酰半胱氨酸(NAC)和冷应激对肉鸡肠道组织形态、能量状况和抗氧化能力的影响.试验将200只1日龄健康雄性科宝肉鸡随机分为4个处理组:对照组(基础日粮)、NAC组(基础日粮+0.1％NAC)、冷应激组(基础日粮)与冷应激+NAC组(基础日粮+0.1％NAC),每组5个重复,每个重复10只鸡.试验第1～2周按常规饲养方法控制温度,试验第3～6周冷应激组和冷应激+NAC组的温度控制在(10±2)℃,而对照组与NAC组维持在(26±2)℃.结果表明:冷应激处理使肉鸡平均日采食量、平均日增重、空肠低氧诱导因子1 (HIF-1) mRNA水平显著下降(P＜0.05),饲料转化率、回肠一磷酸腺苷(AMP)含量和空肠隐窝深度极显著升高(P＜0.01);日粮添加0.1％ NAC显著提高了冷应激肉鸡肠道绒毛高度(VH)、十二指肠与空肠绒毛高度与隐窝深度比值(VH/CD)、空肠三磷酸腺苷(ATP)和腺苷酸池(TAN)含量、回肠ATP和二磷酸腺苷(ADP)及能荷(AEC)含量、十二指肠超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力、回肠谷胱甘肽过氧化物酶(GSH-Px)活力、空肠血红素加氧酶(HMOX) mRNA水平(P＜0.05).因此,日粮中添加0.1％的N-乙酰半胱氨酸可改善肠道组织形态和能量状况,提高冷应激肉鸡肠道抗氧化能力.

语种： 中文

作者： 李鹏;汪中兴;吴梦郡;余魁;纪昌正;...

期刊： 中国畜牧杂志,2018年54(3):87-91 ISSN：0258-7033

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023;武汉轻工大学动物营养与饲料安全湖北省协同创新中心,湖北武汉430023;[余魁; 李鹏; 侯永清; 徐海旺; 吴梦郡; 王蕾; 汪中兴; 易丹; 赵迪; 纪昌正] 武汉轻工大学

关键词： 复合植物精油;脂多糖;仔猪;肝脏

摘要： 试验旨在研究复合植物精油(OCT)对脂多糖(LPS)刺激仔猪肝脏的保护作用.选取18头仔猪,随机分为对照组、LPS组和OCT+LPS组,每组6个重复.对照组与LPS组饲喂基础日粮,OCT+LPS组在基础日粮中添加50 mg/kg OCT.试验期21 d.于试验第21天,LPS组与OCT+LPS组仔猪腹腔注射LPS,对照组注射等量的生理盐水,3h后采血,6h后屠宰.结果表明:与对照组相比,LPS刺激提高了血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷酰转肽酶(GGT)、肝脏诱导型一氧化氮合酶(i-NOS)的活力(P＜0.05),肝脏热休克蛋白70 (HSP70)基因的相对表达量显著提高(P＜0.05);仔猪肝脏表皮生长因子(EGF)、白细胞介素10 (IL-10)、胰岛素样因子(IGF-1)、丝氨酸蛋白激酶(mTOR)基因的相对表达量显著降低(P＜0.05).在日粮中添加50 mg/kg的OCT,可缓解LPS导致的仔猪血浆ALT、AST、GGT活力的升高以及肝脏i-NOS活力的升高(P＜0.05),且有缓解LPS导致的仔猪肝脏MPO活力升高的趋势(P＜0.1),另外可缓解LPS导致的仔猪肝脏HSP70基因相对表达量的升高以及EGF、IL-10、IGF-1、mTOR等基因相对表达量的降低(P＜0.05).综上可知,日粮中添加50 mg/kg的OCT可缓解LPS刺激引起的肝脏炎症反应,提高仔猪肝脏细胞的生长和增殖,进而缓解LPS刺激导致的仔猪肝脏氧化应激损伤.

语种： 中文