作者： Li, Ping;He, Lang;Liu, Xueling;Fan, Shengxuan;Yuan, Yifan;...

期刊： BIOMEDICAL MATERIALS,2021年16(3):035019 ISSN：1748-6041

通讯作者： Wang, Haibo

作者机构： [Li, Ping] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Yuan, Yifan; Liu, Xueling; Li, Sheng; Fan, Shengxuan; Wang, Haibo; He, Lang; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： Biological characterization;Chitosan;Collagen;Electro-deposition;Tube-like hydrogel

摘要： Electro-deposition is a smart, safe and efficient method for biomaterial manufacturing. Collagen, a functional protein with excellent biocompatibility and biosafety, is a promising candidate for tissue engineering and biomedical applications. However, there are few reports on electro-deposition of biomaterials using collagen without electrically or magnetically active nanoparticles. In this study, electro-deposition was employed to swiftly fabricate tube-like collagen–chitosan hydrogels in a mild environment. Fourier transform infrared spectroscopy was employed to analyze the ingredients of the tube-like hydrogels. The result showed that the hydrogels contained both collagen and chitosan. The distribution and content of collagen in the hydrogels was further measured by hematoxylin–eosin staining and hydroxyproline titration. Collagen was distributed homogeneously and its content was related to the initial collagen:chitosan ratio. The tension resistance of the composite gels and the thermal stability of collagen in the composites were obviously enhanced by the chitosan doping. Meanwhile, the tube-like hydrogels retained a good ability to promote cell proliferation of collagen. This method offers a convenient approach to the design and fabrication of collagen-based materials, which could effectively retain the bioactivity and biosafety of collagen and furnish a new way to enhance the stability of collagen and the tensile strength of collagen-based materials. © 2021 IOP Publishing Ltd

语种： 英文

作者： Cheng, Qunpeng;Xu, Chenxi;Huang, Wenwen;Jiang, Meng;Yan, Juntao;...

期刊： Environmental Technology & Innovation,2020年19:100948 ISSN：2352-1864

通讯作者： Song, Guangsen

作者机构： [Cheng, Qunpeng; Zhang, Jian; Song, Guangsen; Yan, Juntao; Chen, Ken; Huang, Wenwen; Fan, Guozhi; Xu, Chenxi] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430073, Peoples R China.;[Jiang, Meng] Huazhong Univ Sci & Technol, Sch Energy & Power Engn, Wuhan, Peoples R China.;[Xiao, Bo] Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan, Peoples R China.

通讯机构： [Song, Guangsen] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430073, Peoples R China.

关键词： Ammonia;Anaerobic digestion;Biochar;Improvement;Piggery wastewater

摘要： In this study, impact of a rice straw-derived biochar on the anaerobic digestion of piggery wastewater was investigated under different ammonium "stress levels". The results showed that the biogas yield was decreased from 1293.27mL to 402.31 mL and the COD removal rate was decreased from 70.68% to 38.13% with the NH4+-N concentration increasing from 900mg/L to 3500 mg/L. At no ammonium "stress level", the COD removal rate was increased from 70.68% to 83.75%, and biogas yield was increased from 1293.27mL to 2306mL with the increase of biochar dosage from 0g to 15g. While with the addition of biochar increasing from 0g to 15g, the COD removal rate was increased from 38.13% to 70.38% and the biogas yield was increased from 382 mL to 1878 mL at high ammonium "stress level". It indicated that the presence of biochar not only could promote biogas yield but also could release the ammonium inhibition. (c) 2020 Elsevier B.V. All rights reserved.

语种： 英文

作者： Li, Zeshan*;He, Weitao;Xiong, Tao;Wang, Yan;Zhang, Juanjuan;...

期刊： BIORESOURCES,2020年15(3):5871-5885 ISSN：1930-2126

通讯作者： Li, Zeshan

作者机构： [He, Weitao; Guo, Chaoqiang; Shang, Shuang; Li, Jianfen; Lan, Kui; Xiong, Tao; Zhang, Yiran; Li, Zeshan; Wang, Yan; Zhang, Juanjuan] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.

通讯机构： [Li, Zeshan] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.

关键词： Catalytic pyrolysis;LaAlO3;Soybean straw;Syr/gas

摘要： A LaAlO3 carrier was prepared via the sol-gel method, and a Ni/LaAlO3 catalyst was prepared via the homogeneous precipitation method; this catalyst was used for the catalytic pyrolysis of soybean straw for syngas (H2 + CO) production. The analysis of the raw materials (straw) was performed via elemental analysis and industrial analysis. The support and catalyst were characterized and analyzed by X-ray fluorescence spectroscopy, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy, and N2 adsorption-desorption isotherms. The results illustrated that the NiO was uniformly loaded on the LaAlO3 surface. Furthermore, the effects of the Ni loading amount, pyrolysis temperature, holding time, and calcination temperature on the performance of the catalyst were studied. The results showed that the catalyst had the highest increase in production and concentration of H2 and CO when the Ni loading was 10 wt%, the calcination temperature was 500 °C, the reaction temperature was 800 °C, and the holding time was 20 min. Compared with the pyrolysis of straw without a catalyst, the yield of H2 and CO increased from 85 mL/g and 125 mL/g to 238.5 mL/g and 255 mL/g, respectively, and the concentration of H2 and CO increased from 23.5 vol% and 29.4 vol% to 43.1 vol% and 41.9 vol%, respectively. © 2020, North Carolina State University.

语种： 英文

作者： 张剑;梅运军;王明明

期刊： 武汉轻工大学学报,2020年39(06):103-107 ISSN：2095-7386

作者机构： 武汉轻工大学化学与环境工程学院,湖北武汉430023;[张剑; 梅运军; 王明明] 武汉轻工大学

关键词： 化学实验;玻璃仪器;污渍;清洗

摘要： 玻璃仪器的清洗是化学实验一个十分重要的环节。选择合理的去渍方法对保证玻璃仪器的洁净尤为重要。对化学实验中玻璃仪器上常见的污渍来源及其成分进行了梳理,并提出了相应的可借鉴的污渍洁净技术。

语种： 中文

作者： Shen, Wenjuan;Zhang, Juanjuan;Xiao, Menghan;Zhang, Xu;Li, Jianfen*;...

期刊： Applied Surface Science,2020年525:146593 ISSN：0169-4332

通讯作者： Li, Jianfen

作者机构： [He, Weitao; Li, Jianfen; Xiao, Menghan; Jiang, Wei; Qin, Zhenhua; Zhang, Shunxi; He, Yun; Shen, Wenjuan; Yan, Juntao; Zhang, Juanjuan] Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 430023, Peoples R China.;[Zhang, Xu] Cent China Normal Univ, Inst Environm & Appl Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.

通讯机构： [Li, Jianfen] W;Wuhan Polytech Univ, Coll Chem & Environm Engn, Wuhan 430023, Peoples R China.

关键词： Cr(VI) removal;Ethylenediaminetetraacetic acid;Surface erosion;Zero-valent iron

摘要： The wide application of zero-valent iron (ZVI) technology was limited by its slow electron transfer rate because of the dense iron oxide on ZVI surface. In this study, we reported a method to improve the performance of ZVI by ball-milling ZVI with ethylenediaminetetraacetic acid (ZVI-EDTA). The pretreatment of ZVI with EDTA provided acidic sites for ZVI surface corrosion, and thus increased the amount of surface Fe0 from 7.23% to 30.12%. Because of the promoted surface Fe0 amount, the corrosion potential of ZVI-EDTA shifted from −0.66 V to −0.75 V, and the concentration of dissolved ferrous ions and total ions increased, resulting in high efficiency in Cr(VI) removal. This study provides an effective method for enhancing the reactivity of commercial ZVI powder, which could contribute to the wide application of ZVI technology, especially in heavy metal remediation. © 2020

语种： 英文

作者： Wang, Wenxin;Shu, Feiyi;Pan, Lingyu;Huang, Shanshan;Tu, Xiao;...

期刊： Macromolecular Research,2020年28(6):636-643 ISSN：1598-5032

通讯作者： Zhang, Juntao;Wang, Haibo

作者机构： [Xu, Chengzhi; Zhang, Juntao; Wang, Haibo; Li, Yuanyuan; Wang, Wenxin; Li, Ping; Tu, Xiao; Li, Siman; Huang, Shanshan; Pan, Lingyu; Shu, Feiyi] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Sun, Yang] Hubei Univ Arts & Sci, Sch Chem Engn & Food Sci, Xiangyang, Hubei, Peoples R China.

通讯机构： [Zhang, JT; Wang, HB] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Grafting (chemical);Hydrophilicity;Molar ratio;Self assembly;Amino group;Grafting densities;Intrinsic property;Native collagen;Succinic anhydride;Succinylated collagen;Succinylation;Triple helixes;Collagen

摘要： Modification of protein could give their new functionality but would affect their intrinsic property and activity. In this paper, a series of succinylated collagen (SCol(n/1)) (n≥1) were prepared by modification of collagen with succinic anhydride at different molar ratio to amino groups amount of collagen. The impact of grafting density on the intrinsic self-assembly and additional hydrophilicity of succinylated collagen was explored. The results revealed that excessive grafting density of succinylated collagen would improve their hydrophilicity but weaken their self-assembly property, although the triple helix of collagen could be reserved after succinylation. SCol(1/1) (grafting density of 17%) with self-assembly property and good hydrophilicity was chosen to compare with native collagen. Compared to native collagen, thermostability of SCol(1/1) decreased slightly, however, SCol(1/1) could form softer hydrogel, which was more favorable for the proliferation of NIH/3 T3. The present work would help us to further understand the importance of grafting density for the design of modified collagen with intrinsic self-assembly property and additional new functionality. [Figure not available: see fulltext.]. © 2020, The Polymer Society of Korea and Springer.

语种： 英文

作者： Zhang, Juntao;Deng, Fen;Liu, Wei;Huang, Yaozhi;Tu, Xiao;...

期刊： NEW JOURNAL OF CHEMISTRY,2020年44(48):21261-21270 ISSN：1144-0546

通讯作者： Wang, Haibo

作者机构： [Deng, Fen; Xu, Chengzhi; Wei, Benmei; Wang, Haibo; Tu, Xiao; He, Lang; Huang, Yaozhi; Kou, Huizhi; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Liu, Wei] Wuhan Polytecn Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Biocompatibility;Grafting (chemical);Hybrid materials;Attractive strategies;Collagen surface;In-situ polymerization;Modification conditions;Native functions;Polymer conjugate;Temperature-responsive;Thermosensitivities;Collagen;collagen;polymer;animal cell;Article;biocompatibility;collagen fibril;conjugate;controlled study;heat sensitivity;hybrid;NIH 3T3 cell line;nonhuman;polymerization;priority journal;surface property;temperature dependence

摘要： Collagen-polymer conjugates are being developed rapidly based on advancements in both polymer and protein science. The attachment of polymers to proteins can be achieved with the "grafting to" method or the "grafting from" method; the latter is more attractive. Although polymer-based collagen engineering provides an attractive strategy to regulate the native functions of collagen, there are still two important unresolved issues: the harsh modification conditions and the loss of fibrillogenesis property. Herein, a collagen-PNIPAAm conjugate (PCol) was prepared by the "grafting from" method to avoid harsh conditions. The amino groups of collagen were modified by a water-soluble NHS-functionalized ATRP initiator before in situ polymerization of NIPAAm on the collagen surface. On the other hand, the fibrillogenesis of PCol was realized by the induction of natural collagen, inspired by the xenogeneic collagen hybrid fibrils in organisms. Furthermore, the structure, fibrillogenesis, thermosensitivity, and biocompatibility of PCol were explored. This work may be useful in synthesizing optimized formulate collagen-polymer conjugates and helpful for the design of the novel collagen-based hybrid materials.

语种： 英文

作者： Shang, Shuang;Qin, Zhenhua;Lan, Kui;Wang, Yan;Zhang, Juanjuan;...

期刊： BIORESOURCES,2020年15(1):1716-1731 ISSN：1930-2126

通讯作者： Li, Jianfen

作者机构： [Shang, Shuang; He, Weitao; Li, Jianfen; Lan, Kui; Qin, Zhenhua; Xiong, Tao; Wang, Yan; Zhang, Juanjuan] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.

通讯机构： [Li, Jianfen] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.

关键词： Biomass;Catalyst;Gasification;Syngas;Zr-MOF

摘要： A Ni/Zr-MOF catalyst supported on Zr-metal organic framework (Zr-MOF) was prepared by a homogeneous precipitation method and was used in the co-gasification of wet sludge and straw. The Ni/Zr-MOF catalyst was characterized via thermogravimetric, X-ray diffraction, scanning electron microscopy, energy dispersive spectrometry, and Brunauer-Emmett-Teller analyses. The experimental results illustrated that the Zr-MOF crystals were an octahedral structure with a specific surface area of 806 m2/g, and had mesoporous structure. Nickel was uniformly dispersed on the surface of the catalyst, and most of the Ni/Zr-MOF crystals maintained an octahedral morphology. Compared with non-catalyst biomass gasification, the H2 yield increased from 0.39 mol/kg to 11.87 mol/kg using the Ni/Zr-MOF catalyst at 500 °C. After 10 instances of reuse, the H2 yield was still as high as 10.11 mol/kg. The Ni/Zr-MOF catalyst exhibited high catalytic activity and stability for biomass gasification at low-temperature. © 2020, North Carolina State University.

语种： 英文

作者： Cheng, Qunpeng*;Xu, Chenxi;Huang, Wenwen;Ma, Chao;Fan, Guozhi;...

期刊： FOOD SCIENCE & NUTRITION,2020年8(1):575-583 ISSN：2048-7177

通讯作者： Cheng, Qunpeng

作者机构： [Jian, Zhang; Cheng, Qunpeng; Ma, Chao; Song, Guangsen; Yan, Juntao; Huang, Wenwen; Fan, Guozhi; Xu, Chenxi; Zhang, Yong] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Cheng, Qunpeng] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： preparation;purity;rice husk;white carbon black

摘要： In this paper, rice husk (RH) was used as raw material to prepare white carbon black, and the key technological parameters of preparing white carbon black from RH were studied through single-factor test, orthogonal experiment, and response surface analysis. Meanwhile, the characteristic of white carbon black was also analyzed. Through orthogonal experiment analysis, it was confirmed that the order of factors affecting the purity of white carbon black was calcination temperature>alkali treatment time>final pH>surfactant. Based on the response surface optimization analysis, the optimum parameters for preparation of white carbon black were as follows: calcination temperature 610°C, alkali treatment time of 2.3hr, final pH of 10, CTMAB was used as the surfactant. Under this condition, the purity of silica prepared could be reached to 99.39%, and the particle size was uniform, spherical, and well dispersed, which satisfied the requirements of GB/T 34698-2017 standard. © 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.

语种： 英文

作者： He, Lang;Yang, Jian;Xu, Chengzhi;Li, Sheng;Wei, Benmei;...

期刊： NEW JOURNAL OF CHEMISTRY,2020年44(17):6760-6770 ISSN：1144-0546

通讯作者： Wang, Haibo

作者机构： [Xu, Yuling; Xu, Chengzhi; Li, Sheng; Wei, Benmei; Wang, Haibo; He, Lang; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.;[Yang, Jian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430000, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

关键词： Collagen;Dichroism;Electrophoresis;High resolution transmission electron microscopy;Mammals;Molecular structure;Pore size;Scanning electron microscopy;Shear deformation;Shearing;Sodium dodecyl sulfate;Sulfur compounds;Viscosity;Assembly behaviors;Bovine tendon collagens;Characteristic peaks;Collagen molecules;Equilibrium moduli;Physical modification of proteins;Sodium dodecyl sulfate-polyacrylamide gel electrophoresis;Weak intermolecular interactions;Shearing machines;collagen gel;Article;bovine;chemical structure;circular dichroism;collagen degradation;collagen fiber;controlled study;flow kinetics;investigative procedures;molecular weight;nonhuman;polyacrylamide gel electrophoresis;pore size;porosity;priority journal;scanning electron microscopy;shear rate;shear treatment;time;transmission electron microscopy;triple helix

摘要： Shear treatment is always employed in the extraction, preparation and research of collagen materials. This study is aimed at investigating the effect of the pre-shearing treatment of neutral bovine tendon collagen solution on the molecular structure, assembly behavior and gel properties of collagen. Rheological and assembly degree (AD) tests showed that with the increase in the shear rate or the extension of shear time during the pre-shearing treatment, the equilibrium moduli and AD of collagen gels that assemble from the pretreated samples gradually decreased. Scanning electron microscopy (SEM) results indicated that with the deepening of the pre-shearing treatment (increased shear rate or shear time), the diameter of the resultant collagen fibers gradually decreased, and their pore size and porosity increased as a whole. The viscosity test of the collagen solution obtained after pre-shearing revealed that the viscosities of the samples were lower than that of the sample devoid of pre-shearing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism (CD) analysis manifested that the molecular weights and CD characteristic peaks of the collagen molecules remained unchanged in the sheared solution. However, with the deepening of the pre-shearing treatment, the proportion of α chains in the SDS-PAGE image increased, while the total content of β and γ chains decreased; the triple-helix structure of collagen in the CD spectrum decreased, and the random coiled structure increased. Moreover, transmission electron microscopy (TEM) displayed that the D-periods of the fibers assembled from the sheared samples were also decreased. A mechanism for the effect of the pre-shearing treatment of the collagen solution on the molecular structure, assembly behavior and gel properties of collagen has been proposed. The findings in this work may provide new understanding and theory on the physical modification of proteins and the construction of new materials from polymer systems that are aggregated or assembled based on weak intermolecular interactions. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2020.

语种： 英文

作者： He, Lang;Li, Sheng;Xu, Chengzhi;Wei, Benmei;Zhang, Juntao;...

期刊： Macromolecular Research,2020年28(9):861-868 ISSN：1598-5032

通讯作者： Wang, Haibo

作者机构： [Xu, Yuling; Xu, Chengzhi; Li, Sheng; Zhu, Beirong; Wu, Xilin; Wei, Benmei; Cao, Yang; Wang, Haibo; He, Lang; Huang, Rongrui; Xiong, Zhijin; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.;[Yang, Jian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430000, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

关键词： Amino acids;Biocompatibility;Collagen;Composite materials;Crosslinking;Degradation;Elastic moduli;Electrophoresis;Hydrogels;Mammals;Sodium dodecyl sulfate;Sulfur compounds;Swelling;Temperature;Tendons;Cold waters;Collagen composites;Composite hydrogels;Composites material;Gel properties;Gelatin-collagen composite hydrogel;Lows-temperatures;Self-healing properties;Sol'gel;Sol-gel property;Sol-gels

摘要： Pure collagen materials are expensive with poor mechanical properties, which need modifications in most cases. As the degradation product of collagen, gelatin is cheap, degradable and biocompatible, but few literatures have reported the research about gelatin-collagen composite materials. This is because gelatin and collagen have different soluble temperatures—gelatin is soluble in hot water (≥30 °C) and swells in cold water. However, a low temperature (2–10 °C) is required to prepare and store collagen solution, and neutral collagen solution denatures quickly above the room temperature. In this study, gelatin was ground into powders and swelled in neutral bovine tendon pepsin-soluble collagen solution (BPSC) to form a homogeneous gelatin-collagen mixture, in light of the swelling characteristics of gelatin in cold water. The assembly properties and gel properties of this composite material were further studied. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) test results showed that the bovine tendon collagen had typical type-I collagen structural characterizations with two α chains of about 100 kDa and one β chain of about 200 kDa; while the SDS-PAGE pattern of gelatin displayed bands continuously distributed from 30 to 200 kDa. Amino acid composition analysis test indicated that the content of polar amino acids and the sum of acidic and base amino acids for gelatin were higher than that of BPSC. Studies on gel properties demonstrated that gelatin-collagen mixed solution had collagen-like assembly characteristics and assembly kinetics. The moduli of the assembled gel at 35 °C were equivalent to that of pure bovine tendon collagen system; moreover, the system moduli didn’t change with time with elastic moduli (G′) of about 40 Pa. However, at 25 °C, the moduli of gelatin-collagen composite hydrogel increased with the extension of time, its G′ increased about 18 times within 8 h, and the ratio of elastic modulus to viscous modulus (G″) increased 4.6 times, showing a significant aging effect of structural strength. Meanwhile, the mechanical strength of the composite hydrogel was also regulated by temperature—the gel was highly elastic (G′≈3,000 Pa, G′>>G″) at a low temperature (5 °C); as the temperature rose, the system moduli gradually decreased and the elastic gel transformed into waterlike fluid at 50 °C little by little. What’s more, gelatin-collagen composite hydrogel also had reversible sol-gel performances and self-healing capability similar to the gelatin hydrogel. This novel preparation method for preparing composite materials and the resultant composite hydrogel are expected to be used in the fields of natural food gels, injectable hydrogels, cell scaffolds, drug sustained-release materials and so on, and improve and promote the processing performances, price and large-scale production of collagen-based materials.[Figure not available: see fulltext.]. © 2020, The Polymer Society of Korea and Springer.

语种： 英文

作者： Sun, Man;Wei, Xu;Wang, Haibo*;Xu, Chengzhi*;Wei, Benmei;...

期刊： Food and Bioprocess Technology,2020年13(2):367-378 ISSN：1935-5130

通讯作者： Wang, Haibo;Xu, Chengzhi

作者机构： [Sun, Man] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Li, Sheng; Wang, Haibo; He, Lang; Xu, CZ; Wei, Xu; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, HB; Xu, CZ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Collagen;Structure restoration;Thermal denaturation;Ultrahigh pressure

摘要： Thermal denaturation is the primary reason for the loss of excellent performance for collagen-based materials. It is particularly urgent to explore an effective structure restoration strategy for thermally denatured collagen. In this study, three different degrees of collagen thermal denaturation models were constructed; the effects of ultrahigh pressure treatments of varying intensities on the structure and properties of partially thermal denatured collagen were investigated. Those results showed that ultrahigh pressure partially restores the triple helix conformation and the α helix of low thermally denatured collagen molecules and moderately restores collagen thermal stability. However, this recovery is not a 'perfect' structural restoration; it cannot effectively restore the fibrotic ability of collagen which leads to a decrease in the fiber diameter of the product and an increase in the storage modulus of the product gel. As the degree of thermal denaturation of collagen increases, the ultrahigh pressure recovery effect is gradually lost. For moderate thermally denatured collagen, ultrahigh pressure treatments only recover a small amount of its triple helical conformation. For high thermally denatured collagen, ultrahigh pressure treatments exacerbate the destruction of the three-dimensional structure of collagen molecules, thereby losing the order of molecular structure and the capability of fibrosis self-assembly. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

语种： 英文

作者： 柴波;杨晓鸿;张剑

期刊： 创新创业理论研究与实践,2019年2(23):102-103 ISSN：2096-5206

作者机构： 武汉轻工大学化学与环境工程学院,湖北武汉 430023;[张剑; 柴波; 杨晓鸿] 武汉轻工大学

关键词： 材料化学;创新创业;人才培养

摘要： 在高等学校创新创业教育改革的背景下,对武汉轻工大学材料化学专业创新创业人才培养模式从人才培养方案修订,教学方法、教学手段和教学内容改革,实施本科生导师制等措施进行了一些改革和探索,期望为其他高等学校创新创业教育改革提供一些借鉴。

语种： 中文

作者： 朱清华;张剑;刘清云;夏志刚;张建

期刊： 清洗世界,2019年35(4):48-51 ISSN：1671-8909

作者机构： 武汉轻工大学化学与环境工程学院;长江大学化学与环境工程学院;[张建; 夏志刚] 江汉油田江汉采油厂;[刘清云] 长江大学;[张剑; 朱清华] 武汉轻工大学

关键词： 注水管网;腐蚀;结垢;油田细菌;油层伤害

摘要： 本文针对江汉油田注入水硫酸盐还原菌超标,管网腐蚀断裂、结垢堵塞与油层伤害严重等生产现状,通过对注入泵、沿线站点及井口水样水化学成分与水质指标检测,结合堵塞、断裂管线垢物成分分析结果,研究管网腐蚀与结垢机理。结果表明,江汉油田注入水SO42-高达4398.2mg/l,水体中有机物和30℃的水温利于硫酸盐还原菌生长繁殖,SRB代谢产物H2S是导致注水管网腐蚀、结垢与油层伤害的主要原因,生产危害极大。

语种： 中文

作者： Wei, Benmei;Wang, Linjie;Zhong, Huaying;Zhang, Juntao;Xu, Chengzhi;...

期刊： NEW JOURNAL OF CHEMISTRY,2019年43(29):11679-11683 ISSN：1144-0546

通讯作者： Wang, Haibo

作者机构： [Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Zhong, Huaying; Li, Sheng; Wang, Linjie; Wang, Haibo; He, Lang; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Hubei, Peoples R China.

关键词： atelocollagen;collagen;fluorescein isothiocyanate;telopeptide;tropocollagen;Article;collagen fibril;extracellular matrix;grass carp;morphology;nonhuman;pig;polyacrylamide gel electrophoresis;priority journal;scanning electron microscopy;skin;viscoelasticity

摘要： Xenogeneic collagen co-assembly (XCCA) is a novel and efficient strategy for improving and regulating the performance of collagen assembled products. However, the effect of collagen molecular structures on XCCA has long been neglected and remains unclear. Herein, we investigated the telopeptide effect of collagen on XCCA by employing grass carp skin collagen (GCSC) with two different structures (tropocollagen and atelocollagen) and porcine skin collagen (PSC). Kinetic analysis showed that the co-assembly rate of PSC and grass carp skin atelocollagen (AC-GCSC) was faster than that of PSC and grass carp skin tropocollagen (TC-GCSC). Based on this observation, we further obtained different concentration dependences of XCCA by the alteration of telopeptide. Compared with PSC/TC-GCSC co-assembly, PSC/AC-GCSC co-assembly exhibited a higher sensitivity and a lower detection limit. Furthermore, telopeptide promoted the fabrication of thicker fibrils and improved the mobility of gels.

语种： 英文

作者： Zhang, Jian*;Song, Guangsen;Mei, Yunjun;Li, Rui;Zhang, Haiyan;...

期刊： CZECH JOURNAL OF FOOD SCIENCES,2019年37(3):141-154 ISSN：1212-1800

通讯作者： Zhang, Jian

作者机构： [Zhang, Jian; Song, Guangsen; Mei, Yunjun; Li, Rui; Zhang, Haiyan; Liu, Ye] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China

通讯机构： [Zhang, Jian] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： alpha-galactosidase;autoclaving;cooking;germination;soaking;alpha-galactose oligosaccharides

摘要： Raffinose family oligosaccharides (RFOs) are alpha-galactosyl derivatives of sucrose or glucose. They are found in a large variety of seeds from many different families such as beans, vegetables and whole grains. Due to absence of alpha-galactosidase in the digestive tract of humans and other monogastric animals, RFOs are responsible for intestinal disturbances (flatulence) following the ingestion of legume-derived products. Structural relationships of RFOs and their enzymatic degradation mechanism are described. Concentration and distribution from various seed sources are introduced. The present status on removal of the RFOs (such as soaking, cooking, germination, and addition of alpha-galactosidase) is summarized. At the meantime, alpha-galactosidases from botanic and microbial sources and their partial enzymatic properties are also presented in detail. Based on a comparison of various removal treatments, the microbial a-galactosidases are thought as the most optimum candidate for removing RFOs in legumes, and the ideal system for the RFO removal is proposed.

语种： 英文

作者： 陶瑞东;王鑫;高欣仪;周银;张剑

期刊： 安徽化工,2019年45(2):41-43 ISSN：1008-553X

作者机构： 武汉轻工大学化学与环境工程学院,湖北 武汉,430023;[周银; 张剑; 陶瑞东; 高欣仪; 王鑫] 武汉轻工大学

关键词： 地衣芽孢杆菌;葡萄糖;生长速率

摘要： 研究了水环境对地衣芽孢杆菌生长的影响。结果表明,37℃、pH=5.0（或9.0）的水环境最有利于地衣芽孢杆菌生长。水环境中一定量的NO3--N对地衣芽孢杆菌生长有促进作用。0.1 g/L的地衣芽孢杆菌接种量,NO3--N浓度为46.7 mg/L最适其生长。地衣芽孢杆菌在生长过程中能缓慢利用HPO42-,经过6天的培养,水环境中的HPO42-由0.98 mg/m L降至0.7 mg/m L。

语种： 中文

作者： Yang, Jian;Wang, Haibo*;He, Lang*;Wei, Benmei;Xu, Chengzhi;...

期刊： Macromolecular Research,2019年27(11):1124-1135 ISSN：1598-5032

通讯作者： Wang, Haibo;He, Lang

作者机构： [Yang, Jian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430000, Hubei, Peoples R China.;[Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Li, Sheng; Wang, Haibo; He, Lang; Zhang, Juntao; He, L] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

通讯机构： [Wang, HB; He, L] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430000, Hubei, Peoples R China.

关键词： Cell proliferation;Differential scanning calorimetry;Gels;Hydrophilicity;Mammals;Morphology;Quenching;Tendons;Viscoelasticity;Bovine tendon collagens;Grass carp skin;reconstituted fibril;regulation;Viscoelastic properties;Collagen

摘要： Heterotypic collagen fibril has long been found in the tissues of organisms, which plays an important role in the formation and function of complex structures of organisms. Inspiring by the phenomenon, scholars tried to incubate collagens from different sources into novel collagen materials in vitro, and the forming of heterogenic reconstituted collagen fibrils (RF) was often demonstrated by differential scanning calorimetry (DSC) and fluorescence quenching analysis. In this work, we used two type-I collagens from different species (bovine tendon and grasscarp fish skin) to co-assemble in vitro, and verified the formation of RF from a new rheological perspective. In addition, we also investigated the assembly behavior, surface hydrophilicity and hydrophobicity, microscopic morphology and cell proliferation ability of the RF. The results showed that the assembly rate, surface properties, fibril size, viscoelastic properties of RF can be delicately regulated by the method of heterogenic collagen reconstitution. This study provides new experimental evidence for the reconstitution of heterogenic collagens, and also offers a new means for the regulation of collagen gel performance, which would help to expand the application range of collagen gel materials. [Figure not available: see fulltext.] © 2019, The Polymer Society of Korea and Springer.

语种： 英文

作者： Zhang, Juntao;Tu, Xiao;Wang, Wenxin;Nan, Jie;Wei, Benmei;...

期刊： International Journal of Biological Macromolecules,2019年128:885-892 ISSN：0141-8130

通讯作者： Wang, Haibo

作者机构： [Xu, Yuling; Xu, Chengzhi; Wei, Benmei; Li, Sheng; Wang, Wenxin; Wang, Haibo; Tu, Xiao; He, Lang; Zhang, Juntao; Nan, Jie] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, Haibo] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： Acrylic acid-grafted-collagen;Grafting density;Self-assembly

摘要： Side chain modification of collagen provides an attractive way to enhance their structure and functions, which is highly desirable for the development of promising biomaterials. However, the impact of structural change of side chains on the intrinsic self-assembly property of collagen was always ignored. Here, a series of acrylic acid-grafted-collagen (AA-g-Col) with different grafting density were prepared to explore the impact of side chain structural variation on the self-assembly of collagen. The results showed that excessive grafting density would weaken or even disappear the self-assembly property of AA-g-Col, but only affects the triple helix to a minor extent. Compared to pristine collagen, the mechanical property and cytocompatibility of AA-g-Col based matrices also deteriorated, along with the increase of grafting density. Therefore, this work contributed a new insight into the importance of grafting density for the study of modified collagen, which would be helpful for the design of optimized formulate collagen-based hybrid materials with both additional novel functions and tissue-mimicking fibrillary structures.

语种： 英文

作者： Shu, Feiyi;Dai, Chun;Wang, Haibo*;Xu, Chengzhi*;Wie, Benmei;...

期刊： ChemistrySelect,2019年4(45):13370-13379 ISSN：2365-6549

通讯作者： Wang, Haibo;Xu, Chengzhi

作者机构： [Xu, Yuling; Xu, Chengzhi; Li, Sheng; Wang, Haibo; Wie, Benmei; He, Lang; Shu, Feiyi; Xu, CZ; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.;[Dai, Chun] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.

通讯机构： [Wang, HB; Xu, CZ] W;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.

关键词： amino acids;collagen;self-assembly;stability;triple helix conformation

摘要： <jats:title>Abstract</jats:title><jats:p>The unique sequence of the α‐chain is the structural cornerstone of the collagen triple helix that dictates its conformation and molecular behavior. In this study, collagen‐like peptides of varying sequence characteristics were designed to elucidate the role of Ser (polar residues), Ala (non‐polar residues), and Arg/Glu (ionizable residues) in the formation, kinetics, and stability of the triple helix, and its self‐assembly behavior. We found that the introduction of other common amino acids in (Gly‐Hyp‐Pro)<jats:sub>n</jats:sub> results in varying degrees of decrease in the rate of formation and stability of the triple helix conformation and the self‐assembly into higher order structures. The introduction of Ser showed a maximal detrimental effect on these processes, followed by the Arg/Glu and Ala. Collagen‐like peptides with Ser could not form thermodynamically stable triple helix conformations and lost their ability to further self‐assemble. Arg/Glu promoted the self‐assembly of collagen‐like peptides, and electrostatic interactions and hydrogen bonding were key to the self‐assembly of periodic ordered structures similar to natural collagen. The hydrophobic interactions of Ala also accelerated self‐assembly to some extent, however, due to the lack of directionality of hydrophobic interactions, the formation of the long‐range ordered structure was affected negatively.</jats:p>

语种： 英文