宠物用茵陈蒿汤配方胶囊的制备及质量标准研究
作者：
周朗;丁力;邱银生;刘宇
作者机构：
武汉轻工大学动物科学与营养工程学院
会议名称：
中国畜牧兽医学会兽医药理毒理学分会第十三次全国会员代表大会暨第十七次学术研讨会
会议时间：
2023-10-15
会议地点：
中国广西南宁
关键词：
制备工艺;干浸膏;成型率;润湿剂
摘要：
<正>引言/目的当前我国的宠物保有量超过2亿,且呈不断上升的趋势。宠物肝脏疾病约占疾病总数的20%,基数庞大,急需肝脏疾病治疗和保健的药物。茵陈蒿汤作为我国经典的方剂之一,应用于人类肝病临床祛黄清热保肝,但其在宠物肝脏保健和疾病治疗上的应用鲜见报道。本研究对药材进行入药标准检查,结合传统茵陈蒿汤的水煎方式进行提取和浓缩工艺的优化制备干浸膏,筛选茵陈蒿汤配方胶囊的处方,制备茵陈蒿汤配方胶囊,建立质量标准草案,考察其稳定性,为宠物用茵陈蒿汤配方胶囊的制备提供药学基础和理论依据。
语种：
中文
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EPA and DHA Inhibit Myogenesis and Downregulate the Expression of Muscle-related Genes in C2C12 Myoblasts
作者：
Zhang, Jing;Xu, Xin;Liu, Yan;Zhang, Lin;Odle, Jack;...
期刊：
Genes ,2019年10(1):64 ISSN：2073-4425
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yan; Zhang, Jing; Liu, Yulan; Xu, Xin; Zhang, Lin; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
C2C12;proliferation;differentiation;EPA;DHA
摘要：
This study was conducted to elucidate the biological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cell proliferation, differentiation and gene expression in C2C12 myoblasts. C2C12 were treated with various concentrations of EPA or DHA under proliferation and differentiation conditions. Cell viability was analyzed using cell counting kit-8 assays (CCK-8). The Edu assays were performed to analyze cell proliferation. To analyze cell differentiation, the expressions of myogenic marker genes were determined at the transcriptional and translational levels by qRT-PCR, immunoblotting and immunofluorescence. Global gene expression patterns were characterized using RNA-sequencing. Phosphorylation levels of ERK and Akt were examined by immunoblotting. Cell viability and proliferation was significantly inhibited after incubation with EPA (50 and 100 muM) or DHA (100 muM). Both EPA and DHA suppressed C2C12 myoblasts differentiation. RNA-sequencing analysis revealed that some muscle-related genes were significantly downregulated following EPA or DHA (50 muM) treatment, including insulin-like growth factor 2 (IGF-2), troponin T3 (Tnnt3), myoglobin (Mb), myosin light chain phosphorylatable fast skeletal muscle (Mylpf) and myosin heavy polypeptide 3 (Myh3). IGF-2 was crucial for the growth and differentiation of skeletal muscle and could activate the PI3K/Akt and the MAPK/ERK cascade. We found that EPA and DHA (50 muM) decreased the phosphorylation levels of ERK1/2 and Akt in C2C12 myoblasts. Thus, this study suggested that EPA and DHA exerted an inhibitory effect on myoblast proliferation and differentiation and downregulated muscle-related genes expression.
语种：
英文
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猪腹膜间皮细胞的培养鉴定及副猪嗜血杆菌感染模型的构建
作者：
徐健峰;赵文华;刘宇;邱银生
作者机构：
武汉轻工大学动物科学与营养工程学院
会议名称：
中国畜牧兽医学会兽医药理毒理学分会第十五次学术讨论会
会议时间：
2019-10-13
会议地点：
中国甘肃兰州
关键词：
猪腹膜间皮细胞;副猪嗜血杆菌;炎性因子
摘要：
[目的]体外分离培养猪腹膜间皮细胞,建立副猪嗜血杆菌感染猪腹膜间皮细胞的体外模型,研究副猪嗜血杆菌感染猪腹膜间皮细胞后引起的细胞炎症反应,筛选副猪嗜血杆菌感染猪腹膜间皮细胞的最佳感染时间和最佳感染比。[方法]从猪腹膜中分离猪腹膜间皮细胞,进行体外培养和鉴定。间皮细胞经传代培养,使用梯度浓度的副猪嗜血杆菌感染猪腹膜间皮细胞,于不同时间点收集细胞上清液,提取间皮细胞的RNA。采用ELISA法测定细胞培养上清液中炎性因子（IL-6、TNF-α和IL-1β）的含量;Q-RT PCR法测定细胞炎性因子（IL-6、TNF-α和IL-1β）的mRNA表达情况。[结果]体外培养的猪腹膜间皮细胞,经免疫组化鉴定确认抗波形蛋白抗体（Vim）、抗细胞角蛋白抗体（PCK）呈阳性,抗白细胞CD45抗体（CD45）、抗第Ⅷ因子相关抗体（F8）呈阴性,确认为猪腹膜间皮细胞。副猪嗜血杆菌感染能极显著上调炎性因子的mRNA表达水平,不同感染时间与不同感染比对猪腹膜间皮细胞的刺激反应不同,在6-20h之间随时间和感染比增加呈上升趋势。[结论]确定了副猪嗜血杆菌感染猪腹膜间皮细胞的最佳感染时间和最佳感染比,为研究副猪嗜血杆菌致腹膜炎症的作用机制提供了模型。
语种：
中文
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Baicalin modulates apoptosis via RAGE, MAPK, and AP-1 in vascular endothelial cells during Haemophilus parasuis invasion
作者：
Fu, Shulin;Zhao, Wenhua;Xiong, Chunhong;Guo, Ling;Guo, Jing;...
期刊：
INNATE IMMUNITY ,2019年25(7):420-432 ISSN：1753-4259
通讯作者：
Qiu, Yinsheng;Hu, Chien-An Andy
作者机构：
[Hu, Chien-An Andy; Liu, Yu; Zhao, Wenhua; Hou, Yongqing; Qiu, Yinsheng; Ye, Chun; Wu, Zhongyuan; Fu, Shulin; Xiong, Chunhong; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Jing; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, YS; Hu, CAA] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Baicalin;Haemophilus parasuis;apoptosis;vascular endothelial cells;invasion
摘要：
Glässer’s disease, caused by Haemophilus parasuis, is a chronic disease related to an inflammatory immune response. Baicalin exerts important biological functions. In this study, we explored the protective efficacy of treatment with baicalin and the potential mechanism of activation of the MAPK signaling pathway in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis. H. parasuis stimulated expression of receptor for advanced glycation end products, induced a significant increase in the level of protein kinase-α and protein kinase-δ phosphorylation, and significantly up-regulated ERK, c-Jun N-terminal kinase, and p38 phosphorylation in PAVECs. H. parasuis also up-regulated the levels of apoptotic genes (Bax, C-myc, and Fasl) and the expression levels of c-Jun and c-Fos, and induced S-phase arrest in PAVECs. However, treatment with baicalin inhibited expression of RAGE, suppressed H. parasuis-induced protein kinase-α and protein kinase-δ phosphorylation, reduced ERK, c-Jun N-terminal kinase, and p38 phosphorylation, down-regulated apoptotic genes (Bax, C-myc, and Fasl), attenuated phospho-c-Jun production from the extracellular to the nuclei, and reversed S-phase arrest in PAVECs. In conclusion, baicalin treatment inhibited the MAPK signaling pathway, thereby achieving its anti-inflammatory responses, which provides a new strategy to control H. parasuis infection. © The Author(s) 2019.
语种：
英文
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Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide.
作者：
Wang, Xiuying;Wang, Wenjun;Wang, Longmei;Yu, Cheng;Zhang, Guolong;...
期刊：
FOOD & FUNCTION ,2019年10(1):479-489 ISSN：2042-6496
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Guolong; Zhao, Shengjun; Hu, Chien-An Andy; Liu, Yulan; Wang, Chunwei; Yu, Cheng; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Yu, Cheng; Wang, Wenjun] South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Hubei, Peoples R China.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
摘要：
The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change. Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases. We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model. Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline). After feeding basal or LNT diet for 21 days, pigs were injected with LPS or saline. At 4 h post-injection, pigs were killed and jejunum, ileum and cecal digesta were collected. LNT improved intestinal morphology and barrier function. LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine. In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor. Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels. Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota. These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones. © 2019 The Royal Society of Chemistry.
语种：
英文
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Effect of baicalin-aluminum complexes on fecal microbiome in piglets
作者：
Fu, Shulin;Zhuang, Feng;Guo, Ling;Qiu, Yinsheng* ;Xiong, Jianglin;...
期刊：
International Journal of Molecular Sciences ,2019年20(10):2390 ISSN：1661-6596
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Yu; Xiong, Jianglin; Zhuang, Feng; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Xiong, Jianglin; Zhuang, Feng; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
ABC transporter;aluminum;baicalin;genomic DNA;hydrogenase;nicotinamide nucleotide adenylyltransferase;phosphotransferase;aluminum;antiinfective agent;baicalin;flavonoid;animal experiment;antimicrobial activity;Article;binding site;bioinformatics;biosynthesis;cell adhesion;chemotaxis;controlled study;diarrhea;discriminant analysis;DNA extraction;DNA library;DNA purification;DNA sequence;drug efficacy;enzyme activity;feces analysis;gene expression;gene ontology;metagenomics;microbial community;microbiome;nonhuman;phylogeny;principal component analysis;Scutellaria baicalensis;sequence alignment;sequence analysis;animal;chemistry;diarrhea;drug effect;feces;intestine flora;microbiology;pig;swine disease;veterinary medicine;Aluminum;Animals;Anti-Infective Agents;Diarrhea;Feces;Flavonoids;Gastrointestinal Microbiome;Swine;Swine Diseases
摘要：
The gut microbiome has important effects on gastrointestinal diseases. Diarrhea attenuation functions of baicalin (BA) is not clear. Baicalin–aluminum complexes (BBA) were synthesized from BA, but the BBA’s efficacy on the diarrhea of piglets and the gut microbiomes have not been explored and the mechanism remains unclear. This study has explored whether BBA could modulate the composition of the gut microbiomes of piglets during diarrhea. The results showed that the diarrhea rate reduced significantly after treatment with BBA. BBA altered the overall structure of the gut microbiomes. In addition, the Gene Ontology (GO) enrichment analysis indicated that the functional differentially expressed genes, which were involved in the top 30 GO enrichments, were associated with hydrogenase (acceptor) activity, nicotinamide-nucleotide adenylyltransferase activity, and isocitrate lyase activity, belong to the molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that flagellar assembly, bacterial chemotaxis, lipopolysaccharide biosynthesis, ATP-binding cassette transporters (ABC) transporters, biosynthesis of amino acids, and phosphotransferase system (PTS) were the most enriched during BBA treatment process. Taken together, our results first demonstrated that BBA treatment could modulate the gut microbiomes composition of piglets with diarrhea, which may provide new potential insights on the mechanisms of gut microbiomes associated underlying the antimicrobial efficacy of BBA. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Effects of Baicalin on piglet monocytes involving PKC-MAPK signaling pathways induced by Haemophilus parasuis.
作者：
Ye, Chun;Li, Ruizhi;Xu, Lei;Qiu, Yinsheng* ;Fu, Shulin;...
期刊：
BMC Veterinary Research ,2019年15(1):1-11 ISSN：1746-6148
通讯作者：
Qiu, Yinsheng
作者机构：
[Ye, Chun; Li, Ruizhi; Xu, Lei; Qiu, Yinsheng; Fu, Shulin; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China;[Ye, Chun; Qiu, Yinsheng; Fu, Shulin; Liu, Yu; Wu, Zhongyuan; Hou, Yongqing] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China. Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Apoptosis;Baicalin;Glasser's disease;Haemophilus parasuis;PKC-MAPK
摘要：
BACKGROUND: Haemophilus parasuis (HPS) is the causative agent of Glasser's disease, characterized by arthritis, fibrinous polyserositis and meningitis, and resulting in worldwide economic losses in the swine industry. Baicalin (BA), a commonly used traditional Chinese medication, has been shown to possess a series of activities, such as anti-bacterial, anti-viral, anti-tumor, anti-oxidant and anti-inflammatory activities. However, whether BA has anti-apoptotic effects following HPS infection is unclear. Here, we investigated the anti-apoptotic effects and mechanisms of BA in HPS-induced apoptosis via the protein kinase C (PKC)-mitogen-activated protein kinase (MAPK) pathway in piglet's mononuclear phagocytes (PMNP). RESULTS: Our data demonstrated that HPS could induce reactive oxygen species (ROS) production, arrest the cell cycle and promote apoptosis via the PKC-MAPK signaling pathway in PMNP. Moreover, when BA was administered, we observed a reduction in ROS production, suppression of cleavage of caspase-3 in inducing apoptosis, and inhibition of activation of the PKC-MAPK signaling pathway for down-regulating p-JNK, p-p38, p-ERK, p-PKC-alpha and PKC-delta in PMNP triggered by HPS. CONCLUSIONS: Our data strongly suggest that BA can reverse the apoptosis initiated by HPS through regulating the PKC-MAPK signaling pathway, which represents a promising therapeutic agent in the treatment of HPS infection.
语种：
英文
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Pharmacokinetics of sodium baicalin following intravenous and intramuscular administration to piglets
作者：
Liu, Yu;Zhao, Wenhua;Xu, Jianfeng;Yu, Xiaolin;Ye, Chun;...
期刊：
Journal of Veterinary Pharmacology and Therapeutics ,2019年42(5):580-584 ISSN：0140-7783
通讯作者：
Qiu, Yinsheng
作者机构：
[Liu, Yu; Zhao, Wenhua; Xu, Jianfeng; Yu, Xiaolin; Ye, Chun; Fu, Shulin; Qiu, Yinsheng] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China
通讯机构：
[Qiu, Yinsheng] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
baicalin;antiinfective agent;baicalin;flavonoid;animal experiment;area under the curve;Article;drug absorption;drug bioavailability;drug blood level;drug clearance;drug distribution;drug elimination;drug half life;female;male;nonhuman;pharmacokinetics;piglet;plasma concentration-time curve;priority journal;animal;blood;half life time;intramuscular drug administration;intravenous drug administration;pig;Animals;Anti-Infective Agents;Area Under Curve;Flavonoids;Half-Life;Injections, Intramuscular;Injections, Intravenous;Swine
摘要：
The purpose of this study was to determine the pharmacokinetics of baicalin after intravenous and intramuscular administration of sodium baicalin at 50 mg/kg to piglets. Plasma baicalin levels were determined by high-performance liquid chromatography. The plasma concentration-time data of baicalin for both administration routes were best described by two-compartmental open model. The area under the plasma concentration-time curve and the elimination half-lives were 77.47 +/- 6.14 mu g/ml x h and 1.73 +/- 0.16 hr for intravenous and 64.85 +/- 5.67 mu g/ml x h and 2.42 +/- 0.15 hr for intramuscular administration, respectively. The apparent volume of distribution and body clearance were 1.63 +/- 0.23 L/kg and 2.74 +/- 0.30 L h(-1) kg(-1) for intravenous and 0.51 +/- 0.10 L/kg and 0.78 +/- 0.08 L h(-1) kg(-1) for intramuscular routes, respectively. An intramuscular injection of sodium baicalin in piglets resulted in rapid and complete absorption, with a mean maximal plasma concentration of 77.28 +/- 7.40 mu g/ml at 0.17 hr and a high absolute bioavailability of 83.73 +/- 5.53%.
语种：
英文
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Dietary fish oil supplementation alters liver gene expressions to protect against LPS-induced liver injury in weanling piglets
作者：
Zhang, Jing;Xu, Xin;Zhu, Huiling;Wang, Yang;Hou, Yongqing;...
期刊：
INNATE IMMUNITY ,2019年25(1):60-72 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Wang, Yang; Zhang, Jing; Liu, Yulan; Hou, Yongqing; Xu, Xin; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Fish oil;inflammation;lipopolysaccharide;liver;RNA sequencing;pig
摘要：
Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury. © The Author(s) 2018.
语种：
英文
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Fermented cassava bioethanol waste as substitute of protein in diet for growth performance and carcass evaluation on meat ducks.
作者：
Lei, Long;Feng, Zhi;Li, Qiang;Xue, Xiaoqiang;Zhang, Dandan;...
期刊：
Tropical Animal Health and Production ,2019年51(5):1049-1056 ISSN：0049-4747
通讯作者：
Zhao, Shengjun
作者机构：
[Li, Qiang; Zhao, Shengjun; Lei, Long; Liu, Zhengya; Liu, Yulan; Xue, Xiaoqiang; Feng, Zhi; Zhang, Dandan; Ren, Ying] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhao, Shengjun] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
Digestibility rate;Fermented cassava bioethanol waste;Growth performance;Meat duck;Meat quality
摘要：
The effect of dietary supplementation with fermented cassava bioethanol waste (FCBW) on the growth performance and meat quality was evaluated in 80 15-day-old male Cherry Valley meat ducks with an initial body weight (BW) of 250.67 +/- 7.50 g. The experiment has 5 replications and 4 treatments and 4 ducks per treatment. Four groups (groups I, II, III, IV) supplemented with 0%, 5%, 10%, and 15% FCBW substituted for part of maize, soybean meal, and bran in basal diet and were fed for 29 days; the metabolizable energy and content of lysine in the four groups were equal. The results indicated that there were no significant differences in average daily weight gain and average daily feed intake among the four groups (P > 0.05). The digestibility rate of dry matter, ash, and phosphorus in group IV was significantly lower than that in group I by 5.23%, 6.25%, and 6.40% respectively (P < 0.05), but the digestibility rate of crude fat was significantly higher than that in group I by 8.30% (P < 0.05). No significant differences were presented among different levels of FCBW supplementation in carcass yield, eviscerated carcass yield, and semi-eviscerated carcass yield (P > 0.05), but 5% FCBW can improve the carcass yield relatively. In conclusion, with dietary supplementation of 5% FCBW, a better growth performance in meat ducks could be achieved.
语种：
英文
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Baicalin modulates NF-kappa B and NLRP3 inflammasome signaling in porcine aortic vascular endothelial cells Infected by Haemophilus parasuis Causing Glasser's disease
作者：
Fu, Shulin;Liu, Huashan;Xu, Lei;Qiu, Yinsheng* ;Liu, Yu;...
期刊：
Scientific Reports ,2018年8(1):807 ISSN：2045-2322
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Huashan; Liu, Yu; Hou, Yongqing; Xu, Lei; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yu; Hou, Yongqing; Ye, Chun; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
摘要：
<jats:title>Abstract</jats:title><jats:p><jats:italic>Haemophilus parasuis (H. parasuis)</jats:italic> can cause vascular inflammatory injury, but the molecular basis of this effect remains unclear. In this study,we investigated the effect of the anti-inflammatory, anti-microbial and anti-oxidant agent, baicalin, on the nuclear factor (NF)-κB and NLRP3 inflammasome signaling pathway in pig primary aortic vascular endothelial cells. Activation of the NF-κB and NLRP3 inflammasome signaling pathway was induced in <jats:italic>H. parasuis</jats:italic>-infected cells. However, baicalin reduced the production of reactive oxygen species, apoptosis, and activation of the NF-κB and NLRP3 inflammasome signaling pathway in infected cells. These results revealed that baicalin can inhibit <jats:italic>H. parasuis</jats:italic>-induced inflammatory responses in porcine aortic vascular endothelial cells, and may thus offer a novel strategy for controlling and treating <jats:italic>H. parasuis</jats:italic> infection. Furthermore, the results suggest that piglet primary aortic vascular endothelial cells may provide an experimental model for future studies of <jats:italic>H. parasuis</jats:italic> infection.</jats:p>
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Glycine relieves intestinal injury by maintaining mtor signaling and suppressing AMPK, TLR4, and NOD signaling in weaned piglets after lipopolysaccharide challenge
作者：
Xu, Xiao;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;Liu, Congcong;...
期刊：
International Journal of Molecular Sciences ,2018年19(7):1980 ISSN：1661-6596
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Liu, Congcong; Xu, Xiao; Liu, Yulan; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Dai, Bing; Liu, Xiuting] Zhe Jiang Goshine Test Technol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
adenosine triphosphate;glycine;iron;lipopolysaccharide;mammalian target of rapamycin;messenger RNA;rapamycin;sodium chloride;toll like receptor 4;zinc;AMP-activated protein kinase kinase;glycine;lipopolysaccharide;protein kinase;target of rapamycin kinase;toll like receptor 4;animal experiment;animal model;Article;colitis;controlled study;diet supplementation;Escherichia coli;gene expression;immunoblotting;inflammation;intestine injury;nonhuman;protein degradation;protein expression;real time polymerase chain reaction;RNA extraction;signal transduction;Western blotting;animal;drug effect;genetics;ileum;immunology;intestine mucosa;intraperitoneal drug administration;jejunum;metabolism;pathology;pig;weaning;Animals;Escherichia coli;Glycine;Ileum;Inflammation;Injections, Intraperitoneal;Intestinal Mucosa;Jejunum;Lipopolysaccharides;Models, Animal;Protein Kinases;RNA, Messenger;Swine;Toll-Like Receptor 4;TOR Serine-Threonine Kinases;Weaning
摘要：
This study was conducted to envaluate whether glycine could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial energy status, protein synthesis, and inflammatory response via AMPK, mTOR, TLR4, and NOD signaling pathways. A total of 24 weanling piglets were randomly allotted to 1 of 4 treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 1% glycine; (4) LPS + 2% glycine. After 28 days feeding, piglets were injected intraperitoneally with saline or LPS. The pigs were slaughtered and intestinal samples were collected at 4 h postinjection. The mRNA expression of key genes in these signaling pathways was measured by real-time PCR. The protein abundance was measured by Western blot analysis. Supplementation with glycine increased jejunal villus height/crypt depth ratio. Glycine also increased the jejunal and ileal protein content, RNA/DNA ratio, and jejunal protein/DNA ratio. The activities of citroyl synthetase in ileum, and α-ketoglutarate dehydrogenase complex in jejunum, were increased in the piglets fed diets supplemented with glycine. In addition, glycine decreased the jejunal and ileal phosphorylation of AMPKα, and increased ileal phosphorylation of mTOR. Furthermore, glycine downregulated the mRNA expression of key genes in inflammatory signaling. Meanwhile, glycine increased the mRNA expression of negative regulators of inflammatory signaling. These results indicate that glycine supplementation could improve energy status and protein synthesis by regulating AMPK and mTOR signaling pathways, and relieve inflammation by inhibiting of TLR4 and NOD signaling pathways to alleviate intestinal injury in LPS-challenged piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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Flaxseed Oil Attenuates Intestinal Damage and Inflammation by Regulating Necroptosis and TLR4/NOD Signaling Pathways Following Lipopolysaccharide Challenge in a Piglet Model
作者：
Zhu, Huiling;Wang, Haibo;Wang, Shuhui;Tu, Zhixiao;Zhang, Lin;...
期刊：
MOLECULAR NUTRITION & FOOD RESEARCH ,2018年62(9):e1700814 ISSN：1613-4125
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Hou, Yongqing; Wang, Haibo; Wang, Shuhui; Wang, Chunwei; Tu, Zhixiao; Zhang, Lin; Wang, Xiuying; Chen, Jie; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
flaxseed oil;inflammation;intestinal barrier function;lipopolysaccharide;necroptosis signaling pathway;weaned pigs
摘要：
Scope: Flaxseed oil is a rich source of α-linolenic acid (ALA), which is the precursor of the long-chain n-3 polyunsaturated fatty acids (PUFAs), including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). This study investigates the protective effect of flaxseed oil against intestinal injury induced by lipopolysaccharide (LPS). Materials and results: Twenty-four weaned pigs were used in a 2 × 2 factorial experiment with dietary treatment (5% corn oil vs 5% flaxseed oil) and LPS challenge (saline vs LPS). On day 21 of the experiment, pigs were administrated with LPS or saline. At 2 h and 4 h post-administration, blood samples were collected. After the blood harvest at 4 h, all piglets were slaughtered and intestinal samples were collected. Flaxseed oil supplementation led to the enrichment of ALA, EPA, and total n-3 PUFAs in intestine. Flaxseed oil improved intestinal morphology, jejunal lactase activity, and claudin-1 protein expression. Flaxseed oil downregulated the mRNA expression of intestinal necroptotic signals. Flaxseed oil also downregulated the mRNA expression of intestinal toll-like receptors 4 (TLR4) and its downstream signals myeloid differentiation factor 88 (MyD88), nuclear factor kappa B (NF−κB), and nucleotide-binding oligomerization domain proteins 1, 2 (NOD1, NOD2) and its adapter molecule, receptor-interacting protein kinase 2 (RIPK2). Conclusion: These results suggest that dietary addition of flaxseed oil enhances intestinal integrity and barrier function, which is involved in modulating necroptosis and TLR4/NOD signaling pathways. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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英文
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Dietary modulation of endogenous host defense peptide synthesis as an alternative approach to in-feed antibiotics
作者：
Robinson, Kelsy;Ma, Xi;Liu, Yulan;Qiao, Shiyan;Hou, Yongqing;...
期刊：
动物营养(英文) ,2018年4(2):160-169 ISSN：2405-6545
通讯作者：
Zhang, Guolong
作者机构：
[Zhang, Guolong; Robinson, Kelsy] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Qiao, Shiyan; Ma, Xi] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Zhang, Guolong; Liu, Yulan; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhang, Guolong] O;[Zhang, Guolong] W;Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Host defense peptides;Antibiotic alternatives;Butyrate;Vitamin D;Antibiotics
摘要：
Traditionally, antibiotics are included in animal feed at subtherapeutic levels for growth promotion and disease prevention. However, recent links between in-feed antibiotics and a rise in antibiotic-resistant pathogens have led to a ban of all antibiotics in livestock production by the European Union in January 2006 and a removal of medically important antibiotics in animal feeds in the United States in January 2017. An urgent need arises for antibiotic alternatives capable of maintaining animal health and productivity without triggering antimicrobial resistance. Host defense peptides (HDP) are a critical component of the animal innate immune system with direct antimicrobial and immunomodulatory activities. While in-feed supplementation of recombinant or synthetic HDP appears to be effective in maintaining animal performance and alleviating clinical symptoms in the context of disease, dietary modulation of the synthesis of endogenous host defense peptides has emerged as a cost-effective, antibiotic-alternative approach to disease control and prevention. Several different classes of small-molecule compounds have been found capable of promoting HDP synthesis. Among the most efficacious compounds are butyrate and vitamin D. Moreover, butyrate and vitamin D synergize with each other in enhancing HDP synthesis. This review will focus on the regulation of HDP synthesis by butyrate and vitamin D in humans, chickens, pigs, and cattle and argue for potential application of HDP-inducing compounds in antibiotic-free livestock production.
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Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide
作者：
Qin, Qin;Xu, Xiao;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;...
期刊：
Scientific Reports ,2018年8(1):15124 ISSN：2045-2322
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Qin, Qin; Xu, Xiao; Liu, Yulan; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Dai, Bing; Liu, Xiuting] Zhe Jiang Goshine Test Technol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Nodal Signaling Pathway;IL-1 Receptor-associated Kinase (IRAK1);Myeloid Differentiation Factor (MyD88);Tollip;TNF Receptor Associated Factor 6 (TRAF6)
摘要：
<jats:title>Abstract</jats:title><jats:p>This experiment aimed to explore whether glutamate (Glu) had beneficial effects on intestinal injury caused by <jats:italic>Escherichia coli</jats:italic> LPS challenge via regulating mTOR, TLRs, as well as NODs signaling pathways. Twenty-four piglets were allotted to 4 treatments including: (1) control group; (2) LPS group; (3) LPS + 1.0% Glu group; (4) LPS + 2.0% Glu group. Supplementation with Glu increased jejunal villus height/crypt depth ratio, ileal activities of lactase, maltase and sucrase, and RNA/DNA ratio and protein abundance of claudin-1 in jejunum and ileum. In addition, the piglets fed Glu diets had higher phosphorylated mTOR (Ser<jats:sup>2448</jats:sup>)/total mTOR ratio in jejunum and ileum. Moreover, Glu decreased TNF-α concentration in plasma. Supplementation with Glu also decreased mRNA abundance of jejunal TLR4, MyD88, IRAK1, TRAF6, NOD2 and increased mRNA abundance of ileal Tollip. These results indicate that Glu supplementation may be closely related to maintaining mTOR and inhibiting TLR4 and NOD signaling pathways, and concomitant improvement of intestinal integrity under an inflammatory condition.</jats:p>
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Flaxseed oil improves liver injury and inhibits necroptotic and inflammatory signaling pathways following lipopolysaccharide challenge in a piglet model
作者：
Wang, Longmei;Tu, Zhixiao;Wang, Haibo;Wang, Shuhui;Wang, Xiuying;...
期刊：
Journal of Functional Foods ,2018年46:482-489 ISSN：1756-4646
通讯作者：
Liu, Yulan
作者机构：
[Hu, Chien-An Andy; Liu, Yulan; Wang, Haibo; Wang, Shuhui; Tu, Zhixiao; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Flaxseed oil;Inflammation;Lipopolysaccharide;Liver;Necroptosis;Pigs
摘要：
The hepatoprotective effects of flaxseed oil (FO) were tested in a pig model of lipopolysaccharide (LPS)-induced liver injury. Twenty-four piglets were used in a 2 × 2 factorial design including diet and an LPS challenge. After 3 weeks of feeding with 5% FO or 5% corn oil, pigs were challenged with LPS or saline. FO alleviated LPS-induced morphological liver damage, reduced serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities, and increased claudin-1 protein expression. FO decreased tumor necrosis factor-α interleukin-6 and cyclooxygenase 2 mRNA expression, and heat shock protein 70 mRNA and protein expression. FO downregulated receptor interacting protein kinase (RIP)1, RIP3, and mixed-lineage kinase domain-like protein (MLKL) mRNA expression, RIP1 and RIP3 protein expression, and MLKL phosphorylation. FO downregulated mRNA expression of toll-like receptor 4, nucleotide-binding oligomerization domain protein 1 and multiple downstream signaling molecules, and decreased p38 and extracellular signal-regulated kinase 1/2 phosphorylation. These results indicate that FO inhibits necroptotic and inflammatory signaling pathways to protect the liver from inflammation-related injury. © 2018 Elsevier Ltd
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英文
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Baicalin inhibits Haemophilus Parasuis-induced high-mobility group box 1 release during inflammation
作者：
Fu, Shulin;Liu, Huashan;Chen, Xiao;Qiu, Yinsheng* ;Ye, Chun;...
期刊：
International Journal of Molecular Sciences ,2018年19(5):1307 ISSN：1661-6596
通讯作者：
Qiu, Yinsheng
作者机构：
[Hu, Chien-An Andy; Liu, Huashan; Liu, Yu; Hou, Yongqing; Ye, Chun; Chen, Xiao; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Huashan; Liu, Yu; Hou, Yongqing; Ye, Chun; Chen, Xiao; Qiu, Yinsheng; Wu, Zhongyuan; Fu, Shulin; Guo, Ling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Qiu, Yinsheng] W;[Qiu, Yinsheng] H;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
baicalin;high mobility group B1 protein;lipopolysaccharide;tumor necrosis factor;antiinfective agent;antiinflammatory agent;baicalin;cytokine;flavonoid;high mobility group B1 protein;animal cell;animal model;antiinflammatory activity;antimicrobial activity;Article;blood analysis;CCL4 gene;CCR2 gene;CD14 gene;CXCL9 gene;CYCS gene;enzyme linked immunosorbent assay;gene;gene sequence;Haemophilus parasuis;inflammation;monocyte;Myd88 gene;nonhuman;protein expression;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA extraction;RNA sequence;sequence analysis;STAT3 gene;Western blotting;animal;dose response;drug effect;genetics;Haemophilus infection;Haemophilus parasuis;inflammation;metabolism;nucleotide sequence;pathogenicity;pig;primary cell culture;swine disease;veterinary medicine;Animals;Anti-Infective Agents;Anti-Inflammatory Agents;Base Sequence;Cytokines;Dose-Response Relationship, Drug;Flavonoids;Haemophilus Infections;Haemophilus parasuis;HMGB1 Protein;Inflammation;Monocytes;Primary Cell Culture;Swine;Swine Diseases
摘要：
Haemophilus parasuis (H. parasuis) can cause Glässer’s disease in pigs. However, the molecular mechanism of the inflammation response induced by H. parasuis remains unclear. The high-mobility group box 1 (HMGB1) protein is related to the pathogenesis of various infectious pathogens, but little is known about whether H. parasuis can induce the release of HMGB1 in piglet peripheral blood monocytes. Baicalin displays important anti-inflammatory and anti-microbial activities. In the present study, we investigated whether H. parasuis can trigger the secretion of HMGB1 in piglet peripheral blood monocytes and the anti-inflammatory effect of baicalin on the production of HMGB1 in peripheral blood monocytes induced by H. parasuis during the inflammation response. In addition, host cell responses stimulated by H. parasuis were determined with RNA-Seq. The RNA-Seq results showed that H. parasuis infection provokes the expression of cytokines and the activation of numerous pathways. In addition, baicalin significantly reduced the release of HMGB1 in peripheral blood monocytes induced by H. parasuis. Taken together, our study showed that H. parasuis can induce the release of HMGB1 and baicalin can inhibit HMGB1 secretion in an H. parasuis-induced peripheral blood monocytes model, which may provide a new strategy for preventing the inflammatory disorders induced by H. parasuis. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
作者：
Zhang, Lin;Wang, Xiuying;Chen, Shaokui;Wang, Shuhui;Tu, Zhixiao;...
期刊：
International Journal of Molecular Sciences ,2018年19(11):3697- ISSN：1661-6596
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Guolong; Liu, Yulan; Chen, Shaokui; Xiong, Jianglin; Wang, Shuhui; Tu, Zhixiao; Li, Xiangen; Zhang, Lin; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
alanine aminotransferase;alkaline phosphatase;aspartate aminotransferase;caspase recruitment domain protein 4;claudin 1;docosahexaenoic acid;heat shock protein 70;icosapentaenoic acid;interleukin 1beta;interleukin 6;lipopolysaccharide;medium chain triacylglycerol;messenger RNA;mitogen activated protein kinase 1;mitogen activated protein kinase 3;mitogen activated protein kinase p38;mixed lineage kinase domain like protein;phosphoglycerate mutase;polyunsaturated fatty acid;protein serine threonine kinase;toll like receptor 4;tumor necrosis factor;unclassified drug;alanine aminotransferase;alkaline phosphatase;antiinflammatory agent;caspase recruitment domain protein 4;cytokine;lipopolysaccharide;omega 3 fatty acid;toll like receptor 4;triacylglycerol;animal experiment;animal model;animal tissue;Article;autophagosome;cell infiltration;controlled study;diet supplementation;down regulation;enzyme linked immunosorbent assay;factorial design;histology;lipid composition;liver injury;membrane rupture;mitochondrion swelling;necroptosis;nonhuman;pig;protein expression;protein phosphorylation;real time polymerase chain reaction;renal protection;signal transduction;transmission electron microscopy;Western blotting;animal;apoptosis;blood;dietary supplement;drug effect;genetics;liver cell;liver disease;MAPK signaling;metabolism;randomization;Alanine Transaminase;Alkaline Phosphatase;Animals;Anti-Inflammatory Agents;Apoptosis;Cytokines;Dietary Supplements;Fatty Acids, Omega-3;Hepatocytes;Lipopolysaccharides;Liver Diseases;MAP Kinase Signaling System;Nod1 Signaling Adaptor Protein;Random Allocation;Swine;Toll-Like Receptor 4;Triglycerides
摘要：
This study was conducted to investigate whether medium-chain triglycerides (MCTs) attenuated lipopolysaccharide (LPS)-induced liver injury by down-regulating necroptotic and inflammatory signaling pathways. A total of 24 pigs were randomly allotted to four treatments in a 2 × 2 factorial design including diet (0 and 4% MCTs) and immunological challenge (saline and LPS). After three weeks of feeding with or without 4% MCTs, pigs were challenged with saline or LPS. MCTs led to a significant increase in eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acid concentrations. MCTs attenuated LPS-induced liver injury as indicated by an improvement in liver histomorphology and ultrastructural morphology of hepatocytes, a reduction in serum alanine aminotransferase and alkaline phosphatase activities as well as an increase in claudin-1 protein expression. In addition, MCTs also reduced serum tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 concentrations, liver TNF-α and IL-1β mRNA expression and protein concentrations and enhanced liver heat shock protein 70 protein expression in LPS-challenged pigs. Moreover, MCTs decreased mRNA expression of receptor-interacting serine/threonine-protein kinase (RIP) 3, mixed-lineage kinase domain-like protein (MLKL) and phosphoglycerate mutase 5 and inhibited MLKL phosphorylation in the liver. Finally, MCTs decreased liver mRNA expression of toll-like receptor (TLR) 4, nucleotide-binding oligomerization domain protein (NOD) 1 and multiple downstream signaling molecules. MCTs also suppressed LPS-induced p38 mitogen-activated protein kinase (MAPK) phosphorylation and increased extracellular signal-related kinase 1/2 phosphorylation in the liver. These results indicated that MCTs are capable of attenuating LPS-induced liver damage by suppressing hepatic necroptotic (RIP1/RIP3/MLKL) and inflammatory (TLR4/NOD1/p38 MAPK) signaling pathways. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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英文
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Occurrence of aflatoxin B1 in dairy cow feedstuff and aflatoxin M1 in UHT and pasteurized milk in central China
作者：
Xiong, Jianglin;Xiong, Lili;Zhou, Hualin;Liu, Yulan;Wu, Lingying*
期刊：
Food Control ,2018年92:386-390 ISSN：0956-7135
通讯作者：
Wu, Lingying
作者机构：
[Liu, Yulan; Wu, Lingying; Xiong, Jianglin; Xiong, Lili] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Dept Bioengn, Xiangyang 441100, Hubei, Peoples R China.
通讯机构：
[Wu, Lingying] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Aflatoxin B1;Aflatoxin M1;China;Feedstuff;Milk
摘要：
This study was carried out to evaluate the occurrence of aflatoxin B1 (AFB1) in dairy cow feedstuff and aflatoxin M1 (AFM1) in milk in central China. One hundred and seventy-four samples of dairy cow feedstuff, 111 samples of UHT milk, and 131 samples of pasteurized milk were collected from different provinces in central China from November 2016 to February 2017. The AFB1 levels in feed were determined by HPLC, and the AFM1 levels in milk were determined by ELISA. Approximately 35.1% of feedstuff samples were positive for AFB1, and AFB1 contents in 6.3% and 2.3% of samples were above the legal limit of 20 μg/kg (in the EU) and 30 μg/kg (in China), respectively. Both peanut cake and maize, compared with other feed material, are susceptible to AFB1 contamination. Approximately 73.6% of milk samples were positive for AFM1, with a mean value of 100.0 ng/L. The AFM1 content was above the EU's legal limit of 50 ng/L in 1.8% of UHT milk samples and in 59.5% of pasteurized milk samples, whereas all milk samples were below the legal limit of 500 ng/L in China. © 2018 Elsevier Ltd
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英文
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The effect of dietary asparagine supplementation on energy metabolism in liver of weaning pigs when challenged with lipopolysaccharide
作者：
Kang, Ping;Liu, Yulan* ;Zhu, Huiling;Zhang, Jing;Shi, Haifeng;...
期刊：
ANIMAL BIOSCIENCE ,2018年31(4):548-555 ISSN：2765-0189
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Zhang, Jing; Liu, Yulan; Pi, Dinan; Leng, Weibo; Kang, Ping; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Asparagine;Energy Metabolism;Lipopolysaccharide;Piglets
摘要：
Objective: This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide. Methods: Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected. Results: At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters. Conclusion: The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge. Copyright © 2018 by Asian-Australasian Journal of Animal Sciences.
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