作者： 李乾玉;刘丽萍;刘玉兰;丛欣;刘洋;...

期刊： 中国无机分析化学,2024年14(11):1595-1602 ISSN：2095-1035

作者机构： [陈绍占; 刘洋; 张晶] 北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室;首都医科大学公共卫生学院;[刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室;恩施德源硒材料工程科技有限公司;武汉轻工大学国家富硒农产品加工技术研发专业中心

关键词： 含硒补充剂;鸡;硒;硒形态

摘要： 通过分析三种不同剂量含硒补充剂饲喂的三黄鸡体内总硒及硒形态含量,研究硒在其体内的富集和分布情况,为发展富硒禽肉等功能农产品提供数据参考。采用微波消解-电感耦合等离子体质谱法(ICP-MS)测定经亚硒酸钠、富硒堇叶碎米荠和...展开更多 通过分析三种不同剂量含硒补充剂饲喂的三黄鸡体内总硒及硒形态含量,研究硒在其体内的富集和分布情况,为发展富硒禽肉等功能农产品提供数据参考。采用微波消解-电感耦合等离子体质谱法(ICP-MS)测定经亚硒酸钠、富硒堇叶碎米荠和富硒酵母饲喂的三黄鸡组织中总硒含量;经55℃水浴超声辅助酶解提取,ZORBAX SB-Aq C18色谱柱分离,以10 mmol/L柠檬酸钠+5 mmol/L己烷磺酸钠(含1%甲醇,pH=4.0)为流动相,高效液相色谱与ICP-MS联用技术(HPLC-ICP/MS)测定其肌肉和肝脏中硒酸根[Se(Ⅵ)]、硒代半胱氨酸(SeCys)、甲基硒代半胱氨酸(MeSeCys)和硒代氨酸(SeMet)4种硒形态。分析结果显示,饲喂富硒酵母组的三黄鸡中肌肉和各组织器官中硒含量最高;鸡胸肉和鸡腿肉中主要硒形态为SeMet,肝脏主要硒形态为SeCys,饲喂富硒酵母组肌肉和肝脏中SeMet含量显著高于其他含硒补充剂组。研究结果表明,添加含硒补充剂的种类对三黄鸡组织中硒的富集有影响,饲喂富硒酵母能显著增加禽类组织中硒含量,特别是SeMet的含量,可为富硒禽肉的饲养提供参考。收起

语种： 中文

作者： Zhang, Jing;Xu, Xin;Liu, Yan;Zhang, Lin;Odle, Jack;...

期刊： Genes,2019年10(1):64 ISSN：2073-4425

通讯作者： Liu, Yulan

作者机构： [Liu, Yan; Zhang, Jing; Liu, Yulan; Xu, Xin; Zhang, Lin; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： C2C12;proliferation;differentiation;EPA;DHA

摘要： This study was conducted to elucidate the biological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cell proliferation, differentiation and gene expression in C2C12 myoblasts. C2C12 were treated with various concentrations of EPA or DHA under proliferation and differentiation conditions. Cell viability was analyzed using cell counting kit-8 assays (CCK-8). The Edu assays were performed to analyze cell proliferation. To analyze cell differentiation, the expressions of myogenic marker genes were determined at the transcriptional and translational levels by qRT-PCR, immunoblotting and immunofluorescence. Global gene expression patterns were characterized using RNA-sequencing. Phosphorylation levels of ERK and Akt were examined by immunoblotting. Cell viability and proliferation was significantly inhibited after incubation with EPA (50 and 100 muM) or DHA (100 muM). Both EPA and DHA suppressed C2C12 myoblasts differentiation. RNA-sequencing analysis revealed that some muscle-related genes were significantly downregulated following EPA or DHA (50 muM) treatment, including insulin-like growth factor 2 (IGF-2), troponin T3 (Tnnt3), myoglobin (Mb), myosin light chain phosphorylatable fast skeletal muscle (Mylpf) and myosin heavy polypeptide 3 (Myh3). IGF-2 was crucial for the growth and differentiation of skeletal muscle and could activate the PI3K/Akt and the MAPK/ERK cascade. We found that EPA and DHA (50 muM) decreased the phosphorylation levels of ERK1/2 and Akt in C2C12 myoblasts. Thus, this study suggested that EPA and DHA exerted an inhibitory effect on myoblast proliferation and differentiation and downregulated muscle-related genes expression.

语种： 英文

作者： Guo, Ling;Li, Linna;Zhang, Yang;Fu, Shulin;Zhang, Jing;...

期刊： INNATE IMMUNITY,2019年25(8):491-502 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Zhang, Yang; Zhang, Jing; Wu, Lingying; Liu, Yulan; Li, Linna; Fu, Shulin; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Yang; Zhang, Jing; Wu, Lingying; Liu, Yulan; Fu, Shulin; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Qiao, Mu] Hubei Acad Agr Sci, Key Lab Anim Embryo Engn & Mol Breeding Hubei Pro, Inst Anim Husb & Vet, Wuhan, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Long non-coding RNA;lipopolysaccharide;intestinal inflammation;cam signalling pathway;mTOR signalling pathway

摘要： LPS can induce an inflammatory immune response in the intestine, and long non-coding RNA (lncRNA) is involved in the process of inflammatory disease. However, the biological role of lncRNA in the intestinal inflammation of piglets remains unclear. In this study, the lncRNA expression profile of the ileal mucosa of piglets challenged by LPS was analysed using lncRNA sequencing. In total, 112 novel lncRNAs were predicted, of which 58 were up-regulated and 54 down-regulated following LPS challenge. Expression of 15 selected lncRNAs was validated by quantitative PCR. We further investigated the target genes of lncRNA that were enriched in the signalling pathways involved in the inflammatory immune response by utilising Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes analysis, with cell adhesion molecules and mTOR signalling pathway identified. In addition, the co-expression networks between the differentially expressed lncRNAs and the target mRNAs were constructed, with seven core lncRNAs identified, which also demonstrated that the relationship between lncRNAs and the target genes was highly correlated. Our study offers important information about the lncRNAs of the mucosal immune system in piglets and provides new insights into the inflammatory mechanism of LPS challenge, which might serve as a novel target to control intestinal inflammation.

语种： 英文

作者： Zhang, Jing;Xu, Xin;Zhu, Huiling;Wang, Yang;Hou, Yongqing;...

期刊： INNATE IMMUNITY,2019年25(1):60-72 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Wang, Yang; Zhang, Jing; Liu, Yulan; Hou, Yongqing; Xu, Xin; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Fish oil;inflammation;lipopolysaccharide;liver;RNA sequencing;pig

摘要： Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury. © The Author(s) 2018.

语种： 英文

作者： 张阳;汪龙梅;郭玲;付书林;刘玉兰;...

期刊： 中国畜牧杂志,2018年54(6):38-42 ISSN：0258-7033

作者机构： 动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉430023;[汪龙梅; 陈洪波; 张阳; 郭玲; 张晶; 刘玉兰; 付书林] 武汉轻工大学

关键词： DNA甲基化;重亚硫酸盐测序法;MKRN3基因;CpG岛

摘要： 为揭示猪MKRN3基因启动子区CpG岛在胚胎小肠组织的甲基化模式,本实验以梅山猪-大白猪正反交为模型,以65、100日龄的胚胎小肠组织为研究材料,首先利用生物信息学手段预测猪MKRN3基因的启动子区CpG岛,围绕预测的CpG岛设计引物,采用重亚硫酸盐测序法检测梅山猪、大白猪正反交不同发育时期胚胎小肠组织中目的基因的甲基化水平.结果表明:在大白×梅山65日龄胚胎小肠中,猪MKRN3基因启动子区CpG岛呈现高度甲基化(70.8％),而在梅山×大白65日龄胚胎小肠中呈现低甲基化(8.4％),正反交后代的甲基化模式截然相反;在大白×梅山100日龄胚胎小肠组织中,目的基因CpG岛呈现低甲基化(16.4％),而在梅山×大白100日龄胚胎小肠组织中的平均甲基化水平为37.2％.MKRN3基因启动子区CpG岛的甲基化模式随着正反交、胚胎发育时期不同而呈现出一定变化规律,推测该基因的DNA甲基化具有父母本等位基因偏好性,且在胚胎发育65～100日龄父本或母本等位基因可能会发生明显的去甲基化.本研究结果为进一步阐明猪MKRN3甲基化水平调控其基因表达及印记状态提供一定理论基础.

语种： 中文

作者： Kang, Ping;Liu, Yulan*;Zhu, Huiling;Zhang, Jing;Shi, Haifeng;...

期刊： ANIMAL BIOSCIENCE,2018年31(4):548-555 ISSN：2765-0189

通讯作者： Liu, Yulan

作者机构： [Wu, Huanting; Zhang, Jing; Liu, Yulan; Pi, Dinan; Leng, Weibo; Kang, Ping; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Asparagine;Energy Metabolism;Lipopolysaccharide;Piglets

摘要： Objective: This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide. Methods: Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected. Results: At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters. Conclusion: The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge. Copyright © 2018 by Asian-Australasian Journal of Animal Sciences.

语种： 英文

作者： Guo, Ling;Guo, Jing;Liu, HuaShan;Zhang, Jing;Chen, Xiabing;...

期刊： Journal of Veterinary Medical Science,2018年80(7):1047-1053 ISSN：0916-7250

通讯作者： Fu, Shulin

作者机构： [Zhang, Jing; Liu, HuaShan; Qiu, Yinsheng; Fu, Shulin; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Chen, Xiabing] Wuhan Acad Agr Sci & Technol, Inst Anim Husb & Vet Sci, Wuhan 430208, Hubei, Peoples R China.

通讯机构： [Fu, Shulin] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： polyphenol;virulence factor;animal;chemistry;growth, development and aging;Haemophilus infection;Haemophilus parasuis;pathogenicity;pig;swine disease;tea;veterinary medicine;virulence;Animals;Haemophilus Infections;Haemophilus parasuis;Polyphenols;Swine;Swine Diseases;Tea;Virulence;Virulence Factors

摘要： The bacterium Haemophilus parasuis (H. parasuis) is the primary cause of Glässer’s disease. Currently, there are no effective vaccines that can confer protection against all H. parasuis serovars. Therefore, the present study aimed to investigate the effect of tea polyphenols on growth, expression of virulence-related factors, and biofilm formation of H. parasuis, as well as to evaluate their protective effects against H. parasuis challenge. Our findings demonstrated that tea polyphenols can inhibit H. parasuis growth in a dose-dependent manner and attenuate the biofilm formation of H. parasuis. In addition, tea polyphenols exerted inhibitory effects on the expression of H. parasuis virulence-related factors. Moreover, tea polyphenols could confer protection against a lethal dose of H. parasuis and can reduce pathological tissue damage induced by H. parasuis. In summary, our findings demonstrated the promising use of tea polyphenols as a novel treatment for H. parasuis infection in pigs. © 2018 The Japanese Society of Veterinary Science.

语种： 英文

作者： 汪龙梅;郭玲;刘玉兰;付书林;陈洪波;...

期刊： 华中农业大学学报,2017年36(6):77-82 ISSN：1000-2421

作者机构： 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;华中农业大学, 农业动物遗传育种与繁殖教育部重点实验室, 武汉, 430070;[汪龙梅; 郭玲; 刘玉兰; 付书林; 陈洪波; 张晶] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;[邓昌彦] 华中农业大学, 农业动物遗传育种与繁殖教育部重点实验室, 武汉, 430070

关键词： 猪;内脂率;臀部膘厚;印记基因

摘要： 以梅山猪、大白猪和长白猪为研究对象,筛选出MRKN3基因的单核苷酸多态位点(SNPs)并进行PCR-RFLP验证,利用PCR-BstUI-RFLP对大白×梅山F_2代资源家系(285头)DNA样品酶切分型,将分型结果与猪胴体性状进行关联分析。结果表明:猪MRKN3基因编码区有7个SNPs,分别为293C>G、361G>A、497G>A、630G>A、678T>C、1376C>T、和1407T>A;其中678T>C位点等位基因频率在中外不同猪种中存在差异;猪MRKN3基因678T>C位点与内脂率及臀部膘厚显著相关(P< 0.05);678T>C位点对内脂率具有显性效应(P<0.05),表现为该位点杂合子比纯合子具有更高的内脂率;而该位点对臀部膘厚具有加性效应(P<0.05),表现为等位基因678C的累加具有降低臀部膘厚的效应。因此,猪MKRN3基因的678T>C位点可为猪的分子育种实践提供一定指导作用。

语种： 中文

作者： 张琳;赵为民;王胜军;刘玉兰;张晶

期刊： 中国畜牧杂志,2017年53(5):85-91 ISSN：0258-7033

作者机构： [王胜军; 张晶; 张琳; 刘玉兰] 武汉轻工大学;[赵为民] 江苏省农业科学院畜牧研究所

关键词： 脂多糖;断奶仔猪;骨骼肌;肝脏

摘要： 为探讨12个长链非编码RNA（lnc RNA）在发生炎症反应的仔猪骨骼肌和肝脏组织炎症反应中的差异表达,本研究选取12头（28±3）日龄、体重（9.0±0.7）kg的杜×长×大断奶仔猪,分成2个处理,每个处理6个重复;2个处理组基础饲粮相同,试验第21天,LPS组腹膜注射100μg/kg体重（BW）的LPS,对照组注射等量的生理盐水。于注射LPS或生理盐水4 h后屠宰,取骨骼肌和肝脏样品待测。结果表明：LPS刺激导致骨骼肌和肝脏组织中肿瘤坏死因子-α（TNF-α）、环氧合酶2（COX2）和核苷酸结合寡聚域受体2（NOD2）的m RNA表达量显著提高（P〈0.05）;LPS刺激极显著降低骨骼肌中lnc RNA-36199.1的表达量（P〈0.01）;LPS刺激显著提高肝脏中lnc RNA-MEG3、lnc RNA-RP11-451G4.2、lnc RNA-27609.1、lnc RNA-32021.1、lnc RNA-46606.2和lnc RNA-36199.1的表达量（P〈0.05）,但显著降低lnc RNA-30795.1和lnc RNA-26244.1的表达量（P〈0.05）。本试验研究表明,8种lnc RNA在LPS诱导的断奶仔猪骨骼肌或肝脏的炎症反应中存在差异表达,说明其可能参与不同组织的炎症反应。

语种： 中文

作者： Zhao, Di;Wu, Tao;Yi, Dan;Wang, Lei;Li, Peng;...

期刊： International Journal of Molecular Sciences,2017年18(12):2535 ISSN：1661-6596

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Hou, Yongqing; Wang, Lei; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Zhang, Junmei] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： acidifying agent;alanine aminotransferase;aspartate aminotransferase;calcium;calcium propionate;catalase;choline;gamma glutamyltransferase;glutathione peroxidase;heat shock protein 70;immunoglobulin enhancer binding protein;interleukin 6;interleukin 8;limestone;lipopolysaccharide;lysine;malonaldehyde;methionine;phosphorus;protein;sodium chloride;soybean protein;superoxide dismutase;threonine;toll like receptor 4;tryptophan;tumor necrosis factor;gamma glutamyltransferase;heat shock protein 70;immunoglobulin enhancer binding protein;interleukin 6;lipopolysaccharide;malonaldehyde;probiotic agent;tumor necrosis factor;animal experiment;animal model;animal tissue;Article;bleeding;cell vacuole;controlled study;diarrhea;diet supplementation;energy;enzyme activity;enzyme assay;experimental liver injury;female;fish meal;gene expression;histopathology;Lactobacillus casei;liver histology;liver sinusoid;lymphocytic infiltration;maize;nonhuman;performance;porcine model;reverse transcription polymerase chain reaction;soybean;ultraviolet spectrophotometry;wheat;whey;animal;blood;dietary supplement;liver;liver disease;metabolism;pig;Animals;Dietary Supplements;Female;gamma-Glutamyltransferase;HSP70 Heat-Shock Proteins;Interleukin-6;Lactobacillus casei;Lipopolysaccharides;Liver;Liver Diseases;Malondialdehyde;NF-kappa B;Probiotics;Swine;Tumor Necrosis Factor-alpha

摘要： This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 × 106 cfu/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups receivedintraperitoneal administration of LPS (100 μg/kg body weight), while the control group received the same volume of saline. Blood and liver samples were collected for analysis. Results showed that L. casei supplementation decreased the feed/gain ratio (p = 0.027) and diarrhea incidence (p &lt; 0.001), and attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 0.048), Tumor necrosis factor-alpha (TNF-α) (p = 0.041), and Malondialdehyde (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS also increased (p &lt; 0.05) the mRNA levels for IL-6, IL-8, TNF-α, Toll-like receptors 4 (TLR4), Nuclear factorkB (NF-kB) and Heat shock protein 70 (HSP70) in the liver. The adverse effects of LPS challenge were ameliorated by L. casei supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver injury via reducing pro-inflammatory cytokines and increasing anti-oxidative capacity. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： 张晶;王胜军;刘妍;汪文俊;刘玉兰

期刊： 中国畜牧杂志,2017年53(3):106-111 ISSN：0258-7033

作者机构： 武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;中南民族大学,生命科学学院,湖北武汉430074;[刘妍; 王胜军; 张晶; 刘玉兰] 武汉轻工大学;[汪文俊] 中南民族大学

关键词： 成肌细胞;激活性蛋白激酶C受体1;肌细胞生成素;肌球蛋白重链;RNA干扰

摘要： 本实验旨在研究RNA干扰（RNAi）RACK1基因对C2C12成肌细胞中肌分化标志基因MHC和MyoG表达的影响。将人工合成的靶向RAKC1基因的3条si RNA（1,2,3）转染C2C12细胞,用RT-qPCR方法检测并筛选干扰效率最高的1条siRNA。将筛选出的si RNA转染C2C12成肌细胞并诱导细胞分化,通过RT-qPCR、Western blot和免疫荧光方法在mRNA及蛋白水平检测RACK1基因沉默后对MHC和MyoG表达的影响。此外,Western blot方法检测RACK1基因沉默后对PI3K/Akt和Erk/MAPK通路激活的影响。结果表明：RACK1-si RNA-2干扰效率最高,转染48 h后抑制率可达70%。随后,C2C12细胞转染si RNA-2,诱导分化48h后RTqPCR表明,MHC和MyoG的m RNA表达均显著性下调（P〈0.05）;诱导分化72 h后,Western blot和免疫荧光结果表明MHC和MyoG的蛋白表达明显低于对照组,但AKT和Erk磷酸化水平未见明显变化。上述结果表明,干扰RACK1能显著抑制MHC和MyoG的表达,提示RACK1正向调控C2C12成肌细胞的分化。

语种： 中文

作者： 张琳;刘妍;刘玉兰;张晶

期刊： 中国畜牧杂志,2017年53(1):61-65 ISSN：0258-7033

作者机构： 武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;中南民族大学生命科学学院,湖北武汉430074;[刘妍; 张晶; 张琳; 刘玉兰] 武汉轻工大学

关键词： 二十碳五烯酸;二十二碳六烯酸;成肌细胞;细胞增殖;细胞凋亡

摘要： 本试验旨在研究二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对体外培养的C2C12成肌细胞增殖和凋亡的影响.用不同终浓度DHA和EPA(0、6 25、12 5、25、50、100 μmol/L)分别作用C2C12细胞12、24、48 h后,用CCK-8法检测细胞的增殖情况;用不同终浓度DHA和EPA(0、50、100 μmol/L)分别作用C2C12细胞48 h后,用TUNEL法检测细胞凋亡情况.结果表明:终浓度为50、100 μmol/L的EPA处理细胞48 h,C2C12细胞增殖能力受到显著抑制,但不同浓度DHA处理组细胞的增殖能力无明显变化.此外,EPA处理C2C12细胞后,TUNEL法显示凋亡细胞数增加,且100μmol/L的EPA处理组细胞凋亡最明显.然而,与对照相比,DHA处理组细胞无明显凋亡.上述结果表明,EPA能抑制C2C12成肌细胞增殖并促进其凋亡,而DHA对细胞增殖和凋亡无明显影响.

语种： 中文

作者： Zhang, Jing;Liu, Yu Lan*

期刊： CURRENT PROTEIN & PEPTIDE SCIENCE,2017年18(6):579-588 ISSN：1389-2037

通讯作者： Liu, Yu Lan

作者机构： [Zhang, Jing; Liu, Yu Lan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.

通讯机构： [Liu, Yu Lan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.

关键词： MicroRNAs;muscle fiber type;muscle protein synthesis;myogenesis;signal proteins;SNP

摘要： Pork is one of the most economical sources of animal protein for human consumption. Meat quality is an important economic trait for the swine industry, which is primarily determined by prenatal muscle development and postnatal growth. Identification of the molecular mechanisms underlying skeletal muscle development is a key priority. MicroRNAs (miRNAs) are a class of small noncoding RNAs that have emerged as key regulators of skeletal muscle development. A number of muscle-related miRNAs have been identified by functional gain and loss experiments in mouse model. However, determining miRNA-mRNA interactions involved in pig skeletal muscle still remains a significant challenge. For a comprehensive understanding of miRNA-mediated mechanisms underlying muscle development, miRNAome analyses of pig skeletal muscle have been performed by deep sequencing. Additionally, porcine miRNA single nucleotide polymorphisms have been implicated in muscle fiber types and meat quality. The present review provides an overview of current knowledge on recently identified miRNAs involved in myogenesis, muscle fiber type and muscle protein metabolism. Undoubtedly, further systematic understanding of the functions of miRNAs in pig skeletal muscle development will be helpful to expand the knowledge of basic skeletal muscle biology and be beneficial for the genetic improvement of meat quality traits.

语种： 英文

作者： 唐斌;金德俊;梁辉煌;孙威;张晶;...

期刊： 中国预防兽医学报,2017年39(12):1013-1016 ISSN：1008-0589

作者机构： 武汉轻工大学动物科学与营养工程学院, 生物资源绿色转化湖北省协同创新中心, 湖北, 武汉, 430023;湖北大学生命科学学院, 生物资源绿色转化湖北省协同创新中心, 湖北, 武汉, 430062;武汉轻工大学动物科学与营养工程学院, 湖北, 武汉, 430023;[唐斌; 魏子贡] 武汉轻工大学动物科学与营养工程学院, 生物资源绿色转化湖北省协同创新中心, 湖北, 武汉, 430023;[金德俊; 梁辉煌; 孙威] 湖北大学生命科学学院, 生物资源绿色转化湖北省协同创新中心, 湖北, 武汉, 430062

关键词： 马链球菌兽疫亚种;PK15细胞;粘附

摘要： microRNA分子在调节宿主细胞应对细菌感染的过程中起重要作用。为研究miR-194b-3p对马链球菌兽疫亚种(SeZ)粘附猪肾上皮细胞(PK15)能力的影响,本实验将miR-194b-3pagomir和antagomir分别转染PK15细胞,检测SeZ对PK15细胞粘附能力的变化。结果表明:在PK15细胞中转染miR-194b-3p agomir,SeZ C55138粘附率显著下降;转染miR-194b-3p antagomir, SeZ C55138粘附率显著上升,确定了miR-194b-3p能够显著抑制SeZC55138对PK15细胞的粘附。同时对其机制进行研究,显示PK15细胞表面CD44是miR-194b-3p的一个调控靶基因, miR-194b-3p通过调节CD44的表达参与影响了上述粘附过程。本实验为深入理解SeZ与PK15细胞的互作机制提供了线索。

语种： 中文

作者： 涂治骁;刘玉兰;吴欢听;王秀英;朱惠玲;...

期刊： 中国畜牧杂志,2016年52(11):35-38,54 ISSN：0258-7033

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[康萍; 吴欢听; 涂治骁; 王秀英; 张晶; 朱惠玲; 刘玉兰] 武汉轻工大学

关键词： 仔猪;脂多糖;NODs信号通路

摘要： 本试验旨在研究脂多糖（LPS）刺激对仔猪核苷酸结合寡聚化结构域蛋白（NODs）信号通路关键基因及其负调控因子m RNA表达的影响。选取12头杜×长×大断奶仔猪,分成2个处理组,每个处理组6个重复。试验组注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后,屠宰仔猪,取空肠、回肠、肝脏、背最长肌和腓肠肌,应用实时荧光定量PCR技术测定NODs信号通路关键基因及其负调控因子m RNA表达水平,包括NOD1、NOD2、受体相互作用蛋白激酶2（RIPK2）、核因子-κB（NF-κB）、肿瘤坏死因子-α（TNF-α）、矢车菊苷β1（ACAP1）和Erbb2相互作用蛋白（ERBB2IP）m RNA表达水平。结果表明:与对照组相比,LPS刺激显著提高了肝脏NOD1、NOD2、RIPK2、NF-κB和TNF-α,背最长肌NOD2、RIPK2和TNF-α,腓肠肌NOD2、RIPK2、NF-κB和TNF-α,空肠RIPK2和TNF-α的m RNA表达量（P<0.05）,LPS刺激有提高回肠RIPK2和NF-κB m RNA表达量的趋势（P<0.10）;同时,LPS刺激显著降低了空肠ACAP1和ERBB2IP,回肠和肝脏ACAP1的m RNA的表达量（P<0.05）。这表明,LPS激活仔猪肠道、肝脏和肌肉组织中NODs信号通路关键基因的表达,而抑制其负调控因子ACAP1和ERBB2IP的表达。

语种： 中文

作者： 王胜军;刘妍;刘玉兰;汪文俊;张晶

期刊： 中国畜牧兽医,2016年43(9):2379-2387 ISSN：1671-7236

作者机构： 武汉轻工大学动物营养与饲料科学湖北省重点实验室，武汉 430023;中南民族大学生命科学学院，武汉 430074;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;中南民族大学生命科学学院,武汉,430074;[刘妍; 王胜军; 张晶; 刘玉兰] 武汉轻工大学

关键词： 骨骼肌消耗;蛋白质降解;炎症反应

摘要： 骨骼肌消耗是肌肉蛋白质合成减少和（或）分解代谢加快的结果,可发生于多种疾病状态下,如病原菌感染、创伤、肿瘤等。目前越来越多的研究结果表明系统性炎症在肌肉蛋白质降解过程中扮演着重要角色。作者综述了近年来关于炎症反应导致肌肉蛋白质降解机理的研究进展,主要包括泛素—蛋白酶体途径、自噬溶酶体降解途径及PI3K/Akt、p38/MAPK、NF-κB等多条信号通路,为开发新的方法治疗肌肉降解提供参考。

语种： 中文

作者： 陈少魁;刘玉兰;王海波;王秀英;朱惠玲;...

期刊： 动物营养学报,2016年28(3):891-898 ISSN：1006-267X

作者机构： [陈少魁; 刘玉兰; 王海波; 王秀英; 朱惠玲; 张晶; 王树辉; 涂治骁] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023

关键词： 仔猪;亚麻籽油;脂多糖;肝脏

摘要： 本试验旨在研究亚麻籽油对脂多糖(LPS)刺激仔猪肝脏Toll样受体4(TLR4)和核苷酸结合寡聚化结构域(NOD)信号通路关键基因表达的影响。选取24头断奶仔猪,按体重相近原则随机分为4个组,分别为对照组、LPS组、2.5%亚麻籽油组(2.5%亚麻籽油+LPS)、5.0%亚麻籽油组(5.0%亚麻籽油+LPS),每组6个重复,每个重复1头猪,试验期21 d。试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后屠宰仔猪,取肝脏,测定TLR4和NOD信号通路关键基因及相关炎性介质的mRNA表达水平。结果表明:1)LPS刺激显著提高了肝脏肿瘤坏死因子-α(TNF-α)、环氧酶2(COX2)、热休克蛋白70(HSP70)的mRNA相对表达量(P<0.05),2.5%亚麻籽油可显著降低COX2、TNF-α的mRNA相对表达量(P<0.05),5.0%亚麻籽油可显著降低TNF-α的mRNA相对表达量(P<0.05)。2)LPS刺激显著提高了肝脏TLR4、髓样分化因子88(MyD88)、白细胞介素-1受体相关激酶1(IRAK1)、NOD1、NOD2、受体互作蛋白2(RIPK2)、核因子-κB(NF-κB)的mRNA相对表达量(P<0.05);2.5%亚麻籽油可显著降低NOD1、NOD2的mRNA相对表达量(P<0.05),有降低RIPK2 mRNA相对表达量的趋势(0.05≤P<0.10);5.0%亚麻籽油可显著降低NOD2的mRNA相对表达量(P<0.05)。这表明LPS刺激导致仔猪发生炎症反应,亚麻籽油可能通过抑制NOD信号通路进而缓解肝脏炎症反应。

语种： 中文

作者： 刘妍;梁辉煌;刘玉兰;唐中林;汪文俊;...

期刊： 畜牧兽医学报,2016年47(2):374-380 ISSN：0366-6964

作者机构： 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;中国农业科学院北京畜牧兽医研究所, 北京, 100193;中南民族大学生命科学学院, 武汉, 430074;[刘妍; 梁辉煌; 刘玉兰; 张晶] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;[唐中林] 中国农业科学院北京畜牧兽医研究所, 北京, 100193

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 脂多糖;C2C12肌管细胞;肌肉蛋白降解;MuRF1基因;MAFbx基因

摘要： 以C2C12肌管细胞为试验材料,探讨脂多糖(LPS)诱导的炎症反应对肌管蛋白降解相关基因及信号通路的影响。选取不同质量浓度(10、100、1 000ng?mL-1)的LPS分别刺激C2C12肌管细胞30min和3h,通过RTqPCR检测炎症因子TNF-α和IL-6的基因转录,确定LPS最佳刺激浓度以建立细胞炎症模型。用最佳浓度LPS分别刺激C2C12肌管细胞0min、30min、1h、3h、6h、12h、24h,RT-qPCR检测MAFbx基因和MuRF基因在不同时间点的转录量变化。此外,1 000ng?mL-1 LPS刺激C2C12肌管细胞12h后,Western blot检测AKT/FOXO1、mTOR和P38信号通路相关蛋白质的表达情况。结果表明:LPS刺激C2C12肌管细胞的最佳浓度为1 000ng?mL-1,在作用30min和3h时均能显著上调TNF-α、IL-6的基因转录,而在刺激12和24h时MuRF1、IL-1β、IL-6和TLR4的基因转录显著上调。此外,1 000ng?mL-1 LPS刺激C2C12肌管细胞12h时,仅AKT和FOXO1蛋白磷酸化水平明显降低。基于以上结果,推测LPS通过调控AKT/FOXO1信号通路诱导C2C12肌管细胞MuRF1转录。

语种： 中文

作者： 王胜军;刘妍;汪文俊;刘玉兰;张晶

作者机构： [王胜军; 刘妍; 汪文俊; 刘玉兰; 张晶] 武汉轻工大学动物营养与饲料科学湖北省重点实验室;[王胜军; 刘妍; 汪文俊; 刘玉兰; 张晶] 中南民族大学生命科学学院

会议名称： 中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

会议时间： 2016-10-21

会议地点： 中国湖北武汉

关键词： 成肌细胞;激活性蛋白激酶C受体1;肌细胞生成素;肌球蛋白重链;RAN干扰

摘要： 本试验旨在研究RAN干扰(RNAi)RACK1基因对C2C12成肌细胞中肌分化标志基因MHC和MyoG表达的影响。将人工合成的靶向RAKC1基因的三条siRNA(1,2,3)转染C2C12细胞,用RT-qPCR方法检测并筛选干扰效率最高的一条siRNA。将筛选出的siRNA转染C2C12成肌细胞并诱导细胞分化,通过RT-qPCR、Western blot和免疫荧光方法在mRNA及蛋白水平检测RAC

语种： 中文

作者： Wang, Xiuying;Liu, Yulan*;Wang, Shuhui;Pi, Dingan;Leng, Weibo;...

期刊： British Journal of Nutrition,2016年116(7):1188-1198 ISSN：0007-1145

通讯作者： Liu, Yulan

作者机构： [Zhang, Jing; Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Wang, Shuhui; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

关键词： Asparagine;Lipopolysaccharides;Muscle atrophy;Pro-inflammatory cytokines

摘要： <jats:title>Abstract</jats:title><jats:p>Pro-inflammatory cytokines are critical in mechanisms of muscle atrophy. In addition, asparagine (Asn) is necessary for protein synthesis in mammalian cells. We hypothesised that Asn could attenuate lipopolysaccharide (LPS)-induced muscle atrophy in a piglet model. Piglets were allotted to four treatments (non-challenged control, LPS-challenged control, LPS+0·5 % Asn and LPS+1·0 % Asn). On day 21, the piglets were injected with LPS or saline. At 4 h post injection, piglet blood and muscle samples were collected. Asn increased protein and RNA content in muscles, and decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1). However, Asn had no effect on the protein abundance of MAFbx and MuRF1. In addition, Asn decreased muscle AMP-activated protein kinase (AMPK) <jats:italic>α</jats:italic> phosphorylation, but increased muscle protein kinase B (Akt) and Forkhead Box O (FOXO) 1 phosphorylation. Moreover, Asn decreased the concentrations of TNF-<jats:italic>α</jats:italic>, cortisol and glucagon in plasma, and <jats:italic>TNF-α</jats:italic> mRNA expression in muscles. Finally, Asn decreased mRNA abundance of muscle toll-like receptor (TLR) 4 and nucleotide-binding oligomerisation domain protein (NOD) signalling-related genes, and regulated their negative regulators. The beneficial effects of Asn on muscle atrophy may be associated with the following: (1) inhibiting muscle protein degradation via activating Akt and inactivating AMPK<jats:italic>α</jats:italic> and FOXO1; and (2) decreasing the expression of muscle pro-inflammatory cytokines via inhibiting TLR4 and NOD signalling pathways by modulation of their negative regulators.</jats:p>

语种： 英文