作者： Zhang, Jing;Xu, Xin;Chen, Hongbo;Kang, Ping;Zhu, Huiling;...

期刊： INNATE IMMUNITY,2021年27(2):170-183 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Zhang, Jing; Liu, Yulan; Kang, Ping; Xu, Xin; Chen, Hongbo; Zhu, Huiling] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Ren, Hongyan] Hubei Acad Agr Sci, Hubei Inst Anim Sci & Vet Med Hubei, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan, Peoples R China.;[Liu, Yulan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

关键词： Inflammation;lipopolysaccharide;lncRNA;mRNA;peripheral blood mononuclear cells;pig

摘要： Long non-coding RNAs (lncRNAs) are emerging as key regulators in inflammation. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in PBMCs treated with LPS using the lncRNA-seq technique. In total 43 differentially expressed (DE) lncRNAs and 1082 DE mRNAs were identified in porcine PBMCs after LPS stimulation. Functional enrichment analysis on DE mRNAs indicated these genes were involved in inflammation-related signaling pathways, including cytokine–cytokine receptor interaction, TNF-α, NF-κB, Jak-STAT and TLR signaling pathways. In addition, co-expression network and function analysis identified the potential lncRNAs related to inflammatory response and immune response. The expressions of eight lncRNAs (ENSSSCT00000045208, ENSSSCT00000051636, ENSSSCT00000049770, ENSSSCT00000050966, ENSSSCT00000047491, ENSSSCT00000049750, ENSSSCT00000054262 and ENSSSCT00000044651) were validated in the LPS-treated PBMCs by quantitative real-time PCR (qRT-PCR). In LPS-challenged piglets, we identified that expression of three lncRNAs (ENSSSCT00000051636, ENSSSCT00000049770, and ENSSSCT00000047491) was significantly up-regulated in liver, spleen and jejunum tissues after LPS challenge, which indicated that these lncRNAs might be important regulators for inflammation. This study provides the first lncRNA and mRNA transcriptomic landscape of LPS-mediated changes in porcine PBMCs, which might provide potential insights into lncRNAs involved in regulating inflammation in pigs. © The Author(s) 2021.

语种： 英文

作者： Kang, Ping;Huang, Xingfa;Wan, Zhicheng;Liang, Tianzeng;Wang, Yang;...

期刊： INNATE IMMUNITY,2021年27(1):23-30 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Wang, Yang; Zhang, Jing; Liang, Tianzeng; Liu, Yulan; Kang, Ping; Li, Xiangen; Huang, Xingfa; Wan, Zhicheng; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Liu, Yulan] Wuhan Polytech Univ, Xuefu South Rd 68, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Xuefu South Rd 68, Wuhan 430023, Hubei, Peoples R China.

关键词： MicroRNAs;muscle;inflammation;protein degradation;piglet;lipopolysaccharide

摘要： <jats:p> To test the dynamic changes of the expression of genes and microRNA in the gastrocnemius muscle after LPS challenge, 36 piglets were assigned to a control group (slaughtered 0 h after saline injection) and LPS groups (slaughtered at 1 h, 2 h, 4 h, 8 h, and 12 h after LPS treatment, respectively). After LPS treatment, the mRNA expression of IL-1β, IL-6, and TNF-α reached maximal levels at 1 h, 2 h, and 1 h, respectively ( P &lt; 0.05), and mRNA expression of TLR4, NODs, muscle-specific ring finger 1, and muscle atrophy F-box peaked at 12 h ( P &lt; 0.05). Moreover, the expression of miR-122, miR-135a, and miR-370 reduced at 1 h, 1 h, and 2 h, respectively ( P &lt; 0.05), and miR-34a, miR-224, miR-132, and miR-145 reached maximum expression levels at 1 h, 1 h, 2 h, and 4 h, respectively ( P &lt; 0.05). These results suggested that mRNA expression of pro-inflammatory cytokines was elevated in the early stage, mRNA expression of genes related to TLR4 and NODs signaling pathways and protein degradation increased in the later phase, and the expression of microRNA related to muscle inflammation and protein degradation changed in the early stage after LPS injection. </jats:p>

语种： 英文

作者： 黄兴法;徐鑫;张晶;王秀英;刘玉兰;...

期刊： 中国饲料,2021年(03):24-27+31 ISSN：1004-3314

作者机构： [黄兴法; 汪文俊] 中南民族大学武陵山区特色资源植物种质保护与利用湖北省重点实验室;[徐鑫; 王秀英; 张晶; 刘玉兰; 康萍] 武汉轻工大学动物营养与饲料科学湖北省重点实验室

关键词： 断奶仔猪;腓肠肌;脂多糖;炎症

摘要： 为研究脂多糖（LPS）刺激后不同时间点断奶仔猪腓肠肌相关microRNA（miRNA）基因的变化规律,试验选取（7.1±0.9）kg杜×长×大断奶仔猪42头,随机分为7个处理组,每个处理6头猪,分别于注射LPS之前（0 h）和注射LPS之后1、2、4、8、12、24 h,屠宰仔猪,取腓肠肌测定相关miRNA的表达。结果表明:与对照组相比,LPS刺激后2 h,miRNA-10b的mRNA表达量显著降低（P ＜0.01）,且于12 h达到峰值;LPS刺激后1 h,miRNA-1和miRNA-206的mRNA表达量均显著降低（P ＜0.01）,但miRNA-133a的mRNA表达量无显著变化（P＞ 0.05）。LPS刺激早期可诱导腓肠肌相关基因miRNA-10b、miRNA-1、miRNA-206的m RNA表达量在不同时间点下调,表明这些miRNA可能参与了肌肉炎症早期相关信号通路的调控。

语种： 中文

作者： Xiao, Kan;Xu, Qiao;Liu, Congcong;He, Pengwei;Qin, Qin;...

期刊： INNATE IMMUNITY,2020年26(8):653-665 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Liu, Congcong; Zhang, Jing; Qin, Qin; He, Pengwei; Liu, Yulan; Xiao, Kan; Xu, Qiao; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Zhang, Guolong; Gin, Ashley] Oklahoma State Univ, Dept Anim & Food Sci, Stillwater, OK 74078 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.

关键词： DHA;cell injury;barrier function;necroptosis;IPEC-1

摘要： Long-chain n-3 polyunsaturated fatty acids are known to have beneficial effects on intestinal health. However, the underling mechanisms are largely unknown. The present study was conducted to investigate whether docosahexaenoic acid (DHA) attenuates TNF-alpha-induced intestinal cell injury and barrier dysfunction by modulating necroptosis signalling. Intestinal porcine epithelial cell line 1 was cultured with or without 12.5 mu g/ml DHA, followed by exposure to 50 ng/ml TNF-alpha for indicated time periods. DHA restored cell viability and cell number triggered by TNF-alpha. DHA also improved barrier function, which was indicated by increased trans-epithelial electrical resistance, decreased FD4 flux and increased membrane localisation of zonula occludins (ZO-1) and claudin-1. Moreover, DHA suppressed cell necrosis in TNF-alpha-challenged cells, as shown in the IncuCyte ZOOM (TM) live cell imaging system and transmission electron microscopy. In addition, DHA decreased protein expression of TNF receptor, receptor interacting protein kinase 1, RIP3 and phosphorylation of mixed lineage kinase-like protein, phosphoglycerate mutase family 5, dynamin-related protein 1 and high mobility group box-1 protein. Furthermore, DHA suppressed protein expression of caspase-3 and caspase-8. Collectively, these results indicate that DHA is capable of alleviating TNF-alpha-induced cell injury and barrier dysfunction by suppressing the necroptosis signalling pathway.

语种： 英文

作者： Xiao, Kan;Liu, Congcong;Tu, Zhixiao;Xu, Qiao;Chen, Shaokui;...

期刊： Oxidative Medicine and Cellular Longevity,2020年2020(1):5803639 ISSN：1942-0900

通讯作者： Liu, Yulan

作者机构： [Liu, Congcong; Zhang, Yang; Zhang, Jing; Hu, Chien-An Andy; Liu, Yulan; Chen, Shaokui; Xiao, Kan; Xu, Qiao; Tu, Zhixiao; Wang, Xiuying] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.

关键词： Cell death;Chemical activation;Enzymes;Hydrogen peroxide;Immunology;Oxidation;Pathology;Peroxides;Epithelial cells;Inflammatory response;Jun N-terminal kinase;Lactate dehydrogenase activities;Mitogen activated protein kinase;Protein expressions;Protein translocation;Tumor necrosis factors;Cell signaling;2 (2 amino 3 methoxyphenyl)chromone;3 (4 methylphenylsulfonyl) 2 propenenitrile;4 (4 fluorophenyl) 2 (4 hydroxyphenyl) 5 (4 pyridyl)imidazole;claudin 1;hydrogen peroxide;immunoglobulin enhancer binding protein;interleukin 6;interleukin 8;lactate dehydrogenase;messenger RNA;mitogen activated protein kinase;stress activated protein kinase;tight junction protein;tumor necrosis factor;hydrogen peroxide;immunoglobulin enhancer binding protein;lactate dehydrogenase;messenger RNA;protein kinase inhibitor;stress activated protein kinase;tight junction protein;transcription factor RelA;animal cell;Article;cell damage;cell nucleus;cell viability;controlled study;enzyme activity;enzyme inhibition;enzyme phosphorylation;gene expression;inflammation;intestine injury;IPEC-1 cell line;MAPK signaling;nonhuman;protein expression;upregulation;animal;cell line;cell survival;drug effect;fluorescence;genetics;inflammation;MAPK signaling;metabolism;pathology;phosphorylation;pig;Cells;Enzymes;Hardiness;Hydrogen Peroxide;Oxidation;Pathology;Peroxides;Stresses;Animals;Cell Line;Cell Survival;Fluorescence;Hydrogen Peroxide;Inflammation;JNK Mitogen-Activated Protein Kinases;L-Lactate Dehydrogenase;MAP Kinase Signaling System;NF-kappa B;Phosphorylation;Protein Kinase Inhibitors;RNA, Messenger;Swine;Tight Junction Proteins;Transcription Factor RelA

摘要： Oxidative stress can lead to intestinal cell injury as well as the induction of inflammation. It is not clear whether inflammation is an important factor leading to cell injury caused by oxidative stress. The purpose of this study was to investigate the role of inflammation in intestinal injury caused by hydrogen peroxide (H2O2). Our results revealed that H2O2 stimulation significantly decreased the viability of intestinal porcine epithelial cells (IPEC-1), increased lactate dehydrogenase (LDH) activity, and disrupted the distribution of the tight junction protein claudin-1. H2O2 significantly increased the mRNA expression of interleukin-6 (IL-6), IL-8, and tumor necrosis factor- (TNF-). H2O2 stimulation also led to increased phosphorylation of p38 and jun N-terminal kinase (JNK), and p65 NF-κB protein translocation into the nucleus of IPEC-1 cells. Cells treated with the NF-κB inhibitor (BAY11-7082), the p38 inhibitor (SB202190), or the JNK inhibitor (PD98059) significantly decreased mRNA and protein expression of IL-6, IL-8, and TNF-. However, treatment with mitogen-activated protein kinase (MAPK) or NF-κB inhibitors did not prevent the damage effect on cell viability, LDH activity, or the distribution of claudin-1 in cells challenged with H2O2. In summary, our data demonstrate that activation of the NF-κB and MAPK signaling pathways can contribute to the inflammatory response, but not cell injury, in IPEC-1 cells challenged with H2O2. © 2020 Kan Xiao et al.

语种： 英文

作者： 黄兴法;徐鑫;张晶;王秀英;刘玉兰;...

期刊： 黑龙江畜牧兽医,2020年(13):17-21 ISSN：1004-7034

作者机构： 中南民族大学武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉430074;武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;[黄兴法; 汪文俊] 中南民族大学;[王秀英; 张晶; 刘玉兰; 徐鑫] 武汉轻工大学

关键词： 长链非编码RNA(lncRNA);脂多糖(LPS);胸腺;淋巴结;空肠;断奶仔猪;炎症

摘要： 为了探究脂多糖（LPS）刺激仔猪发生炎症时炎性因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）、lncRNA-44651和lncRNA-45208在仔猪胸腺、淋巴结和空肠的表达差异,试验选取8头杜×长×大健康断奶仔猪并按照是否注射LPS随机分为对照组和LPS组,各组仔猪在饲喂基础饲粮14 d后,分别按体重100μg/kg腹腔注射生理盐水和LPS,4 h后屠宰仔猪并取胸腺、淋巴结和空肠样品置液氮中保存,采用基因表达测定的方法研究仔猪胸腺、淋巴结和空肠样品相关基因的表达情况。结果表明:LPS刺激4 h后,LPS组仔猪胸腺、淋巴结和空肠中炎性因子TNF-α的mRNA相对表达量均无显著变化（P>0.05）,IL-1β的mRNA相对表达量均极显著上调（P<0.01）;LPS组仔猪胸腺中lncRNA-44651的mRNA相对表达量极显著上调（P<0.01）,lncRNA-45208的mRNA相对表达量显著上调（P<0.05）;LPS组仔猪淋巴结中lncRNA-44651和lncRNA-45208的mRNA相对表达量均极显著上调（P<0.01）;LPS组仔猪空肠中lncRNA-44651的mRNA相对表达量极显著上调（P<0.01）,lncRNA-45208的mRNA相对表达量显著下调（P<0.05）。说明lncRNA-44651和lncRNA-45208可能参与了机体的炎症反应,可为后续仔猪机体功能研究提供候选lncRNA。

语种： 中文

作者： 徐鑫;黄兴法;王秀英;汪文俊;朱惠玲;...

期刊： 中国畜牧杂志,2020年(02):84-87 ISSN：0258-7033

作者机构： 1. 武汉轻工大学动物科学与营养工程学院动物营养与饲料科学湖北省重点实验室;2. 中南民族大学生命科学学院武陵山区特色资源植物种质保护与利用湖北省重点实验室

关键词： 脂多糖;肝脏;脾脏;断奶仔猪

摘要： 本试验旨在研究脂多糖(LPS)诱导仔猪发生炎症反应时肝脏和脾脏炎性细胞因子和lncRNA-47491、lncRNA-49770、lncRNA-51636表达的变化。选取8头21日龄、体重(7.15±0.42)kg的杜×长×大三元杂交断奶仔猪,随机分为对照组、LPS组,每个处理4个重复,每个重复1头猪。预试14 d后,LPS组和对照组腹腔分别注射100μg/kg体重的LPS、生理盐水,4 h后屠宰并取肝脏和脾脏组织样待测。结果表明:与对照组相比,LPS刺激使肝脏和脾脏中炎性细胞因子的mRNA表达量显著上调;LPS诱导的炎症反应中lncRNA-47491、lncRNA-49770和lncRNA-51636的表达量均显著上调;对lncRNA调控的顺式靶标基因的预测结果显示,lncRNA-47491和lncRNA-51636的靶基因有TMEM235、PGS1、TK1等,lncRNA-49770的靶基因有SRRM2、CLDN6、TNFRSF12A等,且这些基因均与炎症相关。综上,lncRNA-47491、lncRNA-49770及lncRNA-51636可能参与了机体的炎症反应,且靶标基因的预测提示其可能发挥不同的调控作用。

语种： 中文

作者： Zhang, Jing;Xu, Xin;Huang, Xingfa;Zhu, Huiling;Chen, Hongbo;...

期刊： INNATE IMMUNITY,2020年26(5):435-446 ISSN：1753-4259

通讯作者： Liu, Yulan;Wang, Wenjun

作者机构： [Zhang, Jing; Liu, Yulan; Xu, Xin; Zhu, Huiling; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wang, Wenjun; Huang, Xingfa] South Cent Univ Nationalities, Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan, Peoples R China.;[Wang, Wenjun] Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Peoples R China.

通讯机构： [Liu, Yulan] W;[Wang, Wenjun] H;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Peoples R China.

关键词： PBMCs;LPS;microRNA;small RNA sequencing;pig

摘要： In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and ssc-miR-103 were the top 10 most abundant miRNAs in porcine PBMCs. Through miRNA differential analysis combined with quantitative PCR, we found the expressions of ssc-miR-122, ssc-miR-129b, ssc-miR-17-5p and ssc-miR-152 were significantly changed in porcine PBMCs after LPS stimulation. Furthermore, targets prediction and function analysis indicated a significant enrichment in gene ontology functional categories related to diseases, immunity and inflammation. In conclusion, this study on profiling of miRNAs expressed in LPS-stimulated PBMCs provides an important reference point for future studies on regulatory roles of miRNAs in porcine immune system. © The Author(s) 2020.

语种： 英文

作者： Xiao, Kan;Liu, Congcong;Qin, Qin;Zhang, Yang;Wang, Xiuying;...

期刊： FASEB JOURNAL,2020年34(2):2483-2496 ISSN：0892-6638

通讯作者： Liu, Yulan

作者机构： [Liu, Congcong; Zhang, Yang; Zhang, Jing; Hu, Chien-An Andy; Qin, Qin; Liu, Yulan; Xiao, Kan; Wang, Xiuying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Xuefunan Rd, Wuhan 430023, Hubei, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Lab Dev Nutr, Dept Anim Sci, Raleigh, NC USA.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Xuefunan Rd, Wuhan 430023, Hubei, Peoples R China.

关键词： barrier function;cell injury;deoxynivalenol;necroptosis signaling pathway;n-3 PUFA

摘要： Deoxynivalenol (DON) is one of the most common mycotoxins that contaminates food or feed and cause intestinal damage. Long-chain n-3 polyunsaturated fatty acids (PUFA) such as EPA and DHA exert beneficial effects on intestinal integrity in animal models and clinical trials. Necroptosis signaling pathway plays a critical role in intestinal cell injury. This study tested the hypothesis that EPA and DHA could alleviate DON-induced injury to intestinal porcine epithelial cells through modulation of the necroptosis signaling pathway. Intestinal porcine epithelial cell 1 (IPEC-1) cells were cultured with or without EPA or DHA (6.25-25 mu g/mL) in the presence or absence of 0.5 mu g/mL DON for indicated time points. Cell viability, cell number, lactate dehydrogenase (LDH) activity, cell necrosis, transepithelial electrical resistance (TEER), fluorescein isothiocyanate-labeled dextran 4kDa (FD4) flux, tight junction protein distribution, and protein abundance of necroptosis related signals were determined. EPA and DHA promoted cell growth indicated by higher cell viability and cell number, and inhibited cell injury indicated by lower LDH activity in the media. EPA and DHA also improved intestinal barrier function, indicated by higher TEER and lower permeability of FD4 flux as well as increased proportions of tight junction proteins located in the plasma membrane. Moreover, EPA and DHA decreased cell necrosis demonstrated by live cell imaging and transmission electron microscopy. Finally, EPA and DHA downregulated protein expressions of necroptosis related signals including tumor necrosis factor receptor (TNFR1), receptor interacting protein kinase 1 (RIP1), RIP3, phosphorylated mixed lineage kinase-like protein (MLKL), phosphoglycerate mutase family 5 (PGAM5), dynamin-related protein 1 (Drp1), and high mobility group box-1 protein (HMGB1). EPA and DHA also inhibited protein expression of caspase-3 and caspase-8. These results suggest that EPA and DHA prevent DON-induced intestinal cell injury and enhance barrier function, which is associated with inhibition of the necroptosis signaling pathway.

语种： 英文

作者： Zhang, Jing;Xu, Xin;Liu, Yan;Zhang, Lin;Odle, Jack;...

期刊： Genes,2019年10(1):64 ISSN：2073-4425

通讯作者： Liu, Yulan

作者机构： [Liu, Yan; Zhang, Jing; Liu, Yulan; Xu, Xin; Zhang, Lin; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： C2C12;proliferation;differentiation;EPA;DHA

摘要： This study was conducted to elucidate the biological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cell proliferation, differentiation and gene expression in C2C12 myoblasts. C2C12 were treated with various concentrations of EPA or DHA under proliferation and differentiation conditions. Cell viability was analyzed using cell counting kit-8 assays (CCK-8). The Edu assays were performed to analyze cell proliferation. To analyze cell differentiation, the expressions of myogenic marker genes were determined at the transcriptional and translational levels by qRT-PCR, immunoblotting and immunofluorescence. Global gene expression patterns were characterized using RNA-sequencing. Phosphorylation levels of ERK and Akt were examined by immunoblotting. Cell viability and proliferation was significantly inhibited after incubation with EPA (50 and 100 muM) or DHA (100 muM). Both EPA and DHA suppressed C2C12 myoblasts differentiation. RNA-sequencing analysis revealed that some muscle-related genes were significantly downregulated following EPA or DHA (50 muM) treatment, including insulin-like growth factor 2 (IGF-2), troponin T3 (Tnnt3), myoglobin (Mb), myosin light chain phosphorylatable fast skeletal muscle (Mylpf) and myosin heavy polypeptide 3 (Myh3). IGF-2 was crucial for the growth and differentiation of skeletal muscle and could activate the PI3K/Akt and the MAPK/ERK cascade. We found that EPA and DHA (50 muM) decreased the phosphorylation levels of ERK1/2 and Akt in C2C12 myoblasts. Thus, this study suggested that EPA and DHA exerted an inhibitory effect on myoblast proliferation and differentiation and downregulated muscle-related genes expression.

语种： 英文

作者： Guo, Ling;Li, Linna;Zhang, Yang;Fu, Shulin;Zhang, Jing;...

期刊： INNATE IMMUNITY,2019年25(8):491-502 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Zhang, Yang; Zhang, Jing; Wu, Lingying; Liu, Yulan; Li, Linna; Fu, Shulin; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Yang; Zhang, Jing; Wu, Lingying; Liu, Yulan; Fu, Shulin; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Qiao, Mu] Hubei Acad Agr Sci, Key Lab Anim Embryo Engn & Mol Breeding Hubei Pro, Inst Anim Husb & Vet, Wuhan, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Long non-coding RNA;lipopolysaccharide;intestinal inflammation;cam signalling pathway;mTOR signalling pathway

摘要： LPS can induce an inflammatory immune response in the intestine, and long non-coding RNA (lncRNA) is involved in the process of inflammatory disease. However, the biological role of lncRNA in the intestinal inflammation of piglets remains unclear. In this study, the lncRNA expression profile of the ileal mucosa of piglets challenged by LPS was analysed using lncRNA sequencing. In total, 112 novel lncRNAs were predicted, of which 58 were up-regulated and 54 down-regulated following LPS challenge. Expression of 15 selected lncRNAs was validated by quantitative PCR. We further investigated the target genes of lncRNA that were enriched in the signalling pathways involved in the inflammatory immune response by utilising Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes analysis, with cell adhesion molecules and mTOR signalling pathway identified. In addition, the co-expression networks between the differentially expressed lncRNAs and the target mRNAs were constructed, with seven core lncRNAs identified, which also demonstrated that the relationship between lncRNAs and the target genes was highly correlated. Our study offers important information about the lncRNAs of the mucosal immune system in piglets and provides new insights into the inflammatory mechanism of LPS challenge, which might serve as a novel target to control intestinal inflammation.

语种： 英文

作者： Zhang, Jing;Xu, Xin;Zhu, Huiling;Wang, Yang;Hou, Yongqing;...

期刊： INNATE IMMUNITY,2019年25(1):60-72 ISSN：1753-4259

通讯作者： Liu, Yulan

作者机构： [Wang, Yang; Zhang, Jing; Liu, Yulan; Hou, Yongqing; Xu, Xin; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Fish oil;inflammation;lipopolysaccharide;liver;RNA sequencing;pig

摘要： Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury. © The Author(s) 2018.

语种： 英文

作者： 张阳;汪龙梅;郭玲;付书林;刘玉兰;...

期刊： 中国畜牧杂志,2018年54(6):38-42 ISSN：0258-7033

作者机构： 动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉430023;[汪龙梅; 陈洪波; 张阳; 郭玲; 张晶; 刘玉兰; 付书林] 武汉轻工大学

关键词： DNA甲基化;重亚硫酸盐测序法;MKRN3基因;CpG岛

摘要： 为揭示猪MKRN3基因启动子区CpG岛在胚胎小肠组织的甲基化模式,本实验以梅山猪-大白猪正反交为模型,以65、100日龄的胚胎小肠组织为研究材料,首先利用生物信息学手段预测猪MKRN3基因的启动子区CpG岛,围绕预测的CpG岛设计引物,采用重亚硫酸盐测序法检测梅山猪、大白猪正反交不同发育时期胚胎小肠组织中目的基因的甲基化水平.结果表明:在大白×梅山65日龄胚胎小肠中,猪MKRN3基因启动子区CpG岛呈现高度甲基化(70.8％),而在梅山×大白65日龄胚胎小肠中呈现低甲基化(8.4％),正反交后代的甲基化模式截然相反;在大白×梅山100日龄胚胎小肠组织中,目的基因CpG岛呈现低甲基化(16.4％),而在梅山×大白100日龄胚胎小肠组织中的平均甲基化水平为37.2％.MKRN3基因启动子区CpG岛的甲基化模式随着正反交、胚胎发育时期不同而呈现出一定变化规律,推测该基因的DNA甲基化具有父母本等位基因偏好性,且在胚胎发育65～100日龄父本或母本等位基因可能会发生明显的去甲基化.本研究结果为进一步阐明猪MKRN3甲基化水平调控其基因表达及印记状态提供一定理论基础.

语种： 中文

作者： Kang, Ping;Liu, Yulan*;Zhu, Huiling;Zhang, Jing;Shi, Haifeng;...

期刊： ANIMAL BIOSCIENCE,2018年31(4):548-555 ISSN：2765-0189

通讯作者： Liu, Yulan

作者机构： [Wu, Huanting; Zhang, Jing; Liu, Yulan; Pi, Dinan; Leng, Weibo; Kang, Ping; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

通讯机构： [Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： Asparagine;Energy Metabolism;Lipopolysaccharide;Piglets

摘要： Objective: This experiment was conducted to investigate whether asparagine (Asn) could improve liver energy status in weaning pigs when challenged with lipopolysaccharide. Methods: Forty-eight weaned pigs (Duroc×Large White×Landrace, 8.12±0.56 kg) were assigned to four treatments: i) CTRL, piglets received a control diet and injected with sterile 0.9% NaCl solution; ii) lipopolysaccharide challenged control (LPSCC), piglets received the same control diet and injected with Escherichia coli LPS; iii) lipopolysaccharide (LPS)+0.5% Asn, piglets received a 0.5% Asn diet and injected with LPS; and iv) LPS+1.0% Asn, piglets received a 1.0% Asn diet and injected with LPS. All piglets were fed the experimental diets for 19 d. On d 20, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weights or the same volume of 0.9% NaCl solution based on the assigned treatments. Then the pigs were slaughtered at 4 h and 24 h after LPS or saline injection, and the liver samples were collected. Results: At 24 h after LPS challenge, dietary supplementation with 0.5% Asn increased ATP concentration (quadratic, p<0.05), and had a tendency to increase adenylate energy charges and reduce AMP/ATP ratio (quadratic, p<0.1) in liver. In addition, Asn increased the liver mRNA expression of pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase β (linear, p<0.05; quadratic, p<0.05), and had a tendency to increase the mRNA expression of hexokinase 2 (linear, p<0.1). Moreover, Asn increased liver phosphorylated AMP-activated protein kinase (pAMPK)/total AMP-activated protein kinase (tAMPK) ratio (linear, p<0.05; quadratic, p<0.05). However, at 4 h after LPS challenge, Asn supplementation had no effect on these parameters. Conclusion: The present study indicated that Asn could improve the energy metabolism in injured liver at the late stage of LPS challenge. Copyright © 2018 by Asian-Australasian Journal of Animal Sciences.

语种： 英文

作者： Guo, Ling;Guo, Jing;Liu, HuaShan;Zhang, Jing;Chen, Xiabing;...

期刊： Journal of Veterinary Medical Science,2018年80(7):1047-1053 ISSN：0916-7250

通讯作者： Fu, Shulin

作者机构： [Zhang, Jing; Liu, HuaShan; Qiu, Yinsheng; Fu, Shulin; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Chen, Xiabing] Wuhan Acad Agr Sci & Technol, Inst Anim Husb & Vet Sci, Wuhan 430208, Hubei, Peoples R China.

通讯机构： [Fu, Shulin] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： polyphenol;virulence factor;animal;chemistry;growth, development and aging;Haemophilus infection;Haemophilus parasuis;pathogenicity;pig;swine disease;tea;veterinary medicine;virulence;Animals;Haemophilus Infections;Haemophilus parasuis;Polyphenols;Swine;Swine Diseases;Tea;Virulence;Virulence Factors

摘要： The bacterium Haemophilus parasuis (H. parasuis) is the primary cause of Glässer’s disease. Currently, there are no effective vaccines that can confer protection against all H. parasuis serovars. Therefore, the present study aimed to investigate the effect of tea polyphenols on growth, expression of virulence-related factors, and biofilm formation of H. parasuis, as well as to evaluate their protective effects against H. parasuis challenge. Our findings demonstrated that tea polyphenols can inhibit H. parasuis growth in a dose-dependent manner and attenuate the biofilm formation of H. parasuis. In addition, tea polyphenols exerted inhibitory effects on the expression of H. parasuis virulence-related factors. Moreover, tea polyphenols could confer protection against a lethal dose of H. parasuis and can reduce pathological tissue damage induced by H. parasuis. In summary, our findings demonstrated the promising use of tea polyphenols as a novel treatment for H. parasuis infection in pigs. © 2018 The Japanese Society of Veterinary Science.

语种： 英文

作者： 汪龙梅;郭玲;刘玉兰;付书林;陈洪波;...

期刊： 华中农业大学学报,2017年36(6):77-82 ISSN：1000-2421

作者机构： 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;华中农业大学, 农业动物遗传育种与繁殖教育部重点实验室, 武汉, 430070;[汪龙梅; 郭玲; 刘玉兰; 付书林; 陈洪波; 张晶] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;[邓昌彦] 华中农业大学, 农业动物遗传育种与繁殖教育部重点实验室, 武汉, 430070

关键词： 猪;内脂率;臀部膘厚;印记基因

摘要： 以梅山猪、大白猪和长白猪为研究对象,筛选出MRKN3基因的单核苷酸多态位点(SNPs)并进行PCR-RFLP验证,利用PCR-BstUI-RFLP对大白×梅山F_2代资源家系(285头)DNA样品酶切分型,将分型结果与猪胴体性状进行关联分析。结果表明:猪MRKN3基因编码区有7个SNPs,分别为293C>G、361G>A、497G>A、630G>A、678T>C、1376C>T、和1407T>A;其中678T>C位点等位基因频率在中外不同猪种中存在差异;猪MRKN3基因678T>C位点与内脂率及臀部膘厚显著相关(P< 0.05);678T>C位点对内脂率具有显性效应(P<0.05),表现为该位点杂合子比纯合子具有更高的内脂率;而该位点对臀部膘厚具有加性效应(P<0.05),表现为等位基因678C的累加具有降低臀部膘厚的效应。因此,猪MKRN3基因的678T>C位点可为猪的分子育种实践提供一定指导作用。

语种： 中文

作者： Zhao, Di;Wu, Tao;Yi, Dan;Wang, Lei;Li, Peng;...

期刊： International Journal of Molecular Sciences,2017年18(12):2535 ISSN：1661-6596

通讯作者： Hou, Yongqing

作者机构： [Wu, Tao; Hou, Yongqing; Wang, Lei; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Zhang, Junmei] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

通讯机构： [Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.

关键词： acidifying agent;alanine aminotransferase;aspartate aminotransferase;calcium;calcium propionate;catalase;choline;gamma glutamyltransferase;glutathione peroxidase;heat shock protein 70;immunoglobulin enhancer binding protein;interleukin 6;interleukin 8;limestone;lipopolysaccharide;lysine;malonaldehyde;methionine;phosphorus;protein;sodium chloride;soybean protein;superoxide dismutase;threonine;toll like receptor 4;tryptophan;tumor necrosis factor;gamma glutamyltransferase;heat shock protein 70;immunoglobulin enhancer binding protein;interleukin 6;lipopolysaccharide;malonaldehyde;probiotic agent;tumor necrosis factor;animal experiment;animal model;animal tissue;Article;bleeding;cell vacuole;controlled study;diarrhea;diet supplementation;energy;enzyme activity;enzyme assay;experimental liver injury;female;fish meal;gene expression;histopathology;Lactobacillus casei;liver histology;liver sinusoid;lymphocytic infiltration;maize;nonhuman;performance;porcine model;reverse transcription polymerase chain reaction;soybean;ultraviolet spectrophotometry;wheat;whey;animal;blood;dietary supplement;liver;liver disease;metabolism;pig;Animals;Dietary Supplements;Female;gamma-Glutamyltransferase;HSP70 Heat-Shock Proteins;Interleukin-6;Lactobacillus casei;Lipopolysaccharides;Liver;Liver Diseases;Malondialdehyde;NF-kappa B;Probiotics;Swine;Tumor Necrosis Factor-alpha

摘要： This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 × 106 cfu/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups receivedintraperitoneal administration of LPS (100 μg/kg body weight), while the control group received the same volume of saline. Blood and liver samples were collected for analysis. Results showed that L. casei supplementation decreased the feed/gain ratio (p = 0.027) and diarrhea incidence (p &lt; 0.001), and attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 0.048), Tumor necrosis factor-alpha (TNF-α) (p = 0.041), and Malondialdehyde (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS also increased (p &lt; 0.05) the mRNA levels for IL-6, IL-8, TNF-α, Toll-like receptors 4 (TLR4), Nuclear factorkB (NF-kB) and Heat shock protein 70 (HSP70) in the liver. The adverse effects of LPS challenge were ameliorated by L. casei supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver injury via reducing pro-inflammatory cytokines and increasing anti-oxidative capacity. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： 张琳;赵为民;王胜军;刘玉兰;张晶

期刊： 中国畜牧杂志,2017年53(5):85-91 ISSN：0258-7033

作者机构： [王胜军; 张晶; 张琳; 刘玉兰] 武汉轻工大学;[赵为民] 江苏省农业科学院畜牧研究所

关键词： 脂多糖;断奶仔猪;骨骼肌;肝脏

摘要： 为探讨12个长链非编码RNA（lnc RNA）在发生炎症反应的仔猪骨骼肌和肝脏组织炎症反应中的差异表达,本研究选取12头（28±3）日龄、体重（9.0±0.7）kg的杜×长×大断奶仔猪,分成2个处理,每个处理6个重复;2个处理组基础饲粮相同,试验第21天,LPS组腹膜注射100μg/kg体重（BW）的LPS,对照组注射等量的生理盐水。于注射LPS或生理盐水4 h后屠宰,取骨骼肌和肝脏样品待测。结果表明：LPS刺激导致骨骼肌和肝脏组织中肿瘤坏死因子-α（TNF-α）、环氧合酶2（COX2）和核苷酸结合寡聚域受体2（NOD2）的m RNA表达量显著提高（P〈0.05）;LPS刺激极显著降低骨骼肌中lnc RNA-36199.1的表达量（P〈0.01）;LPS刺激显著提高肝脏中lnc RNA-MEG3、lnc RNA-RP11-451G4.2、lnc RNA-27609.1、lnc RNA-32021.1、lnc RNA-46606.2和lnc RNA-36199.1的表达量（P〈0.05）,但显著降低lnc RNA-30795.1和lnc RNA-26244.1的表达量（P〈0.05）。本试验研究表明,8种lnc RNA在LPS诱导的断奶仔猪骨骼肌或肝脏的炎症反应中存在差异表达,说明其可能参与不同组织的炎症反应。

语种： 中文

作者： 张晶;王胜军;刘妍;汪文俊;刘玉兰

期刊： 中国畜牧杂志,2017年53(3):106-111 ISSN：0258-7033

作者机构： 武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;中南民族大学,生命科学学院,湖北武汉430074;[刘妍; 王胜军; 张晶; 刘玉兰] 武汉轻工大学;[汪文俊] 中南民族大学

关键词： 成肌细胞;激活性蛋白激酶C受体1;肌细胞生成素;肌球蛋白重链;RNA干扰

摘要： 本实验旨在研究RNA干扰（RNAi）RACK1基因对C2C12成肌细胞中肌分化标志基因MHC和MyoG表达的影响。将人工合成的靶向RAKC1基因的3条si RNA（1,2,3）转染C2C12细胞,用RT-qPCR方法检测并筛选干扰效率最高的1条siRNA。将筛选出的si RNA转染C2C12成肌细胞并诱导细胞分化,通过RT-qPCR、Western blot和免疫荧光方法在mRNA及蛋白水平检测RACK1基因沉默后对MHC和MyoG表达的影响。此外,Western blot方法检测RACK1基因沉默后对PI3K/Akt和Erk/MAPK通路激活的影响。结果表明：RACK1-si RNA-2干扰效率最高,转染48 h后抑制率可达70%。随后,C2C12细胞转染si RNA-2,诱导分化48h后RTqPCR表明,MHC和MyoG的m RNA表达均显著性下调（P〈0.05）;诱导分化72 h后,Western blot和免疫荧光结果表明MHC和MyoG的蛋白表达明显低于对照组,但AKT和Erk磷酸化水平未见明显变化。上述结果表明,干扰RACK1能显著抑制MHC和MyoG的表达,提示RACK1正向调控C2C12成肌细胞的分化。

语种： 中文

作者： 张琳;刘妍;刘玉兰;张晶

期刊： 中国畜牧杂志,2017年53(1):61-65 ISSN：0258-7033

作者机构： 武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;中南民族大学生命科学学院,湖北武汉430074;[刘妍; 张晶; 张琳; 刘玉兰] 武汉轻工大学

关键词： 二十碳五烯酸;二十二碳六烯酸;成肌细胞;细胞增殖;细胞凋亡

摘要： 本试验旨在研究二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对体外培养的C2C12成肌细胞增殖和凋亡的影响.用不同终浓度DHA和EPA(0、6 25、12 5、25、50、100 μmol/L)分别作用C2C12细胞12、24、48 h后,用CCK-8法检测细胞的增殖情况;用不同终浓度DHA和EPA(0、50、100 μmol/L)分别作用C2C12细胞48 h后,用TUNEL法检测细胞凋亡情况.结果表明:终浓度为50、100 μmol/L的EPA处理细胞48 h,C2C12细胞增殖能力受到显著抑制,但不同浓度DHA处理组细胞的增殖能力无明显变化.此外,EPA处理C2C12细胞后,TUNEL法显示凋亡细胞数增加,且100μmol/L的EPA处理组细胞凋亡最明显.然而,与对照相比,DHA处理组细胞无明显凋亡.上述结果表明,EPA能抑制C2C12成肌细胞增殖并促进其凋亡,而DHA对细胞增殖和凋亡无明显影响.

语种： 中文