Injury and mechanism of recombinant E-coli expressing STa on piglets colon
作者：
Lv, Yang;Li, Xueni;Zhang, Lin;Shi, Yutao;Du, Linxiao;...
期刊：
Journal of Veterinary Medical Science ,2018年80(2):205-212 ISSN：0916-7250
通讯作者：
Wu, Tao
作者机构：
[Wu, Tao; Gong, Joshua; Lv, Yang; Hou, Yongqing; Li, Xueni; Shi, Yutao; Ding, Binying; Du, Linxiao] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Lin] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构：
[Wu, Tao] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
colon;heat-stable enterotoxin A;piglet;recombinant escherichia coli
摘要：
Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 × 10 9 CFU E. coli LMG194-STa), LMG194 group (2 × 10 9 CFU E. coli LMG194) and K88 group (2 × 10 9 CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H 2 O 2 ; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines. © 2018 The Japanese Society of Veterinary Science.
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Effects of dietary coated-oleum cinnamomi supplementation on the immunity and intestinal integrity of broiler chickens
作者：
Guo, Shuangshuang;Cheng, Qiang;Li, Yehan;Duan, Rui;Hou, Yongqing;...
期刊：
Animal Science Journal ,2018年89(11):1581-1590 ISSN：1344-3941
通讯作者：
Ding, Binying
作者机构：
[Cheng, Qiang; Duan, Rui; Guo, Shuangshuang; Li, Yehan; Hou, Yongqing; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Ding, Binying] W;Wuhan Polytech Univ, Engn Res Ctr Feed Prot Resources Agr By Prod, Minist Educ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
broiler chicken;coated oleum cinnamomi;immunity;intestinal integrity
摘要：
Oleum cinnamomi (OCM) is increasingly used as a feed additive in animal diets. The aim of this study was to investigate the effects of dietary supplementation with coated‐OCM (cOCM) on the immunity and intestinal integrity of broiler chickens. A total of 396 one‐day‐old chicks were randomly assigned into six groups. The basal diets were supplemented with 50 mg/kg of flavomycin (positive control) as well as 0 (control), 50, 100, 200, and 300 mg/kg of cOCM. Compared with the control, both positive control and cOCM treatments did not improve the growth performance. Serum immunoglobulin (Ig) Y levels were decreased by flavomycin and 50 mg/kg of cOCM treatments (p < 0.05). Dietary cOCM decreased ileal secretory IgA contents at d 21 and commonly down‐regulated duodenal and ileal mRNA expression of interleukin (IL)‐1β and IL‐8 at d 42 (p < 0.05). The 300 mg/kg of cOCM increased jejunal ratio of villus height to crypt depth and upregulated intestinal claudin‐1 expression (p < 0.05). Jejunal (at d 21) and duodenal (at d 42) mucin‐2 expression was up and downregulated by both 50 and 300 mg/kg of cOCM, respectively (p < 0.05). In conclusion, dietary cOCM addition helped to maintain noninflammatory states of humoral and mucosal immunity, and improved the intestinal integrity of broiler chickens.
语种：
英文
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Establishment of a recombinant Escherichia coli-induced piglet diarrhea model
作者：
Wu, Tao;Lv, Yang;Li, Xueni;Zhao, Di;Yi, Dan;...
期刊：
FRONTIERS IN BIOSCIENCE-LANDMARK ,2018年23(8):1517-1534 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Gong, Joshua; Lv, Yang; Hou, Yongqing; Wang, Lei; Li, Xueni; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Res & Dev Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Intestinal injury;Piglet Diarrhea;Recombinant Escherichia coli;Review
摘要：
Enterotoxigenic Escherichia coli strains induce human and animal intestinal dysfunction and injury, and cause diarrhea in weanling pigs. A recombinant E.coli strain LMG194-STa which expressed single toxin STa of ETEC was constructed by directly inserting the STa gene of ETEC into the expression vector pBAD202 and then transferring the recombinant plasmid pBAD-STa into the E.coli LMG19 host strain. Diarrhea and intestinal injury in piglets were induced by oral administration of recombinant strain LMG194-Sta. In vitro, the recombinant strain LMG194-Sta had the same toxicity to the IPEC cell as wild type strain K88, and higher toxicity than the host strain LMG194. In vivo, LMG194-STa caused severe diarrhea in piglets as K88, the diarrhea rate of LMG194-STa and K88 groups was higher than that in the LMG194 and control groups. Both K88 and LMG194-STa induced intestinal inflammation, injury, and atrophy, while adversely affecting the expression of genes for cytokines, transporters and ion channels, and nutrient metabolism. Thus, we established a porcine model of recombinant E. coli strain LMG194-STa-induced diarrhea for future nutritional and mechanistic studies of intestinal dysfunction. © 2018 Frontiers in Bioscience. All Rights Reserved.
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Tea polyphenols suppress growth and virulence-related factors of Haemophilus parasuis
作者：
Guo, Ling;Guo, Jing;Liu, HuaShan;Zhang, Jing;Chen, Xiabing;...
期刊：
Journal of Veterinary Medical Science ,2018年80(7):1047-1053 ISSN：0916-7250
通讯作者：
Fu, Shulin
作者机构：
[Zhang, Jing; Liu, HuaShan; Qiu, Yinsheng; Fu, Shulin; Guo, Jing; Guo, Ling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Chen, Xiabing] Wuhan Acad Agr Sci & Technol, Inst Anim Husb & Vet Sci, Wuhan 430208, Hubei, Peoples R China.
通讯机构：
[Fu, Shulin] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
biofilm;Haemophilus parasuis;protection;tea polyphenols;virulence-related factor
摘要：
The bacterium Haemophilus parasuis (H. parasuis) is the primary cause of Glasser's disease. Currently, there are no effective vaccines that can confer protection against all H. parasuis serovars. Therefore, the present study aimed to investigate the effect of tea polyphenols on growth, expression of virulence-related factors, and biofilm formation of H. parasuis, as well as to evaluate their protective effects against H. parasuis challenge. Our findings demonstrated that tea polyphenols can inhibit H. parasuis growth in a dose-dependent manner and attenuate the biofilm formation of H. parasuis. In addition, tea polyphenols exerted inhibitory effects on the expression of H. parasuis virulence-related factors. Moreover, tea polyphenols could confer protection against a lethal dose of H. parasuis and can reduce pathological tissue damage induced by H. parasuis. In summary, our findings demonstrated the promising use of tea polyphenols as a novel treatment for H. parasuis infection in pigs.
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英文
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Bioequivalence evaluation of two 5% ceftiofur hydrochloride sterile suspension in pigs
作者：
Xiong, Jincheng;Zhu, Qianqian;Lei, Zhixin;Yang, Shuaike;Chen, Peiyuan;...
期刊：
Journal of Veterinary Medical Science ,2018年80(12):1847-1852 ISSN：0916-7250
通讯作者：
Cao, Jiyue;Qiu, Yinsheng
作者机构：
[Zhu, Qianqian; Zhao, Yaxin; Lei, Zhixin; Xiong, Jincheng; Yang, Shuaike; Chen, Peiyuan; Cao, Jiyue] Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China.;[Zhu, Qianqian; Zhao, Yaxin; Lei, Zhixin; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Zhu, Qianqian; Zhao, Yaxin; Lei, Zhixin; Xiong, Jincheng; Yang, Shuaike; Cao, Jiyue] Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;[Qiu, Yinsheng] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Cao, Jiyue] H;[Qiu, Yinsheng] W;Huazhong Agr Univ, Dept Vet Pharmacol, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.;Huazhong Agr Univ, Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China.
关键词：
bioequivalence;ceftiofur;confidence interval;pharmacokinetic;pig
摘要：
The purpose of this study was to evaluate the bioequivalence of 5% ceftiofur hydrochloride sterile suspension in two formulations, a test formulation (Saifukang 5% CEF, Hvsen) and a reference formulation (Excenel®RTU 5% CEF, Pfizer). Twenty-four healthy pigs were assigned to a two-period, two-treatment crossover parallel trial, and both formulations were administered at a single intramuscular dose of 5 mg/kg weight, with a 7-day washout period. Blood samples were collected consecutively for up to 144 hr after administration. The concentrations of ceftiofur-and desfuroylceftiofur-related metabolites in the plasma were determined by high-performance liquid chromatography. In addition, the major pharmacokinetic parameters (C max , AUC 0-t and AUC 0-∞ ) were computed and compared via analysis of variance, with 90% confidence intervals. Bioequivalence evaluation of T max was statistically analyzed with the nonparametric test. The comparison values between test and reference formulation for AUC 0-t , AUC 0-∞ , C max , and T max were 376.7 ± 75.3 µg·hr/ml, 390.5 ± 78.6 µg·hr/ml, 385.9 ± 79.2 µg·hr/ml, 402.7 ± 80.4 µg·hr/ml, 34.6 ± 5.5 µg/ml, 36.1 ± 6.2 µg/ml, 1.27 ± 0.18 hr, and 1.26 ± 0.21 hr, respectively, and we observed no significant differences between the two formulations. The 90% CI values were within the recommended range of 80–125% (P>0.05), and the relative bioavailability of the test product was 96.47 ± 10.92% according to AUC 0-t values. Based on our results, the two formulations exhibit comparable pharmacokinetic profiles, and the test product is bioequivalent to the reference formulation. © 2018 The Japanese Society of Veterinary Science.
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英文
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Balantidium grimi n. sp (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China
作者：
Zhao, Weishan;Li, Can;Zhang, Dong;Wang, Runqiu;Zheng, Yingzhen;...
期刊：
PARASITE ,2018年25:29 ISSN：1252-607X
通讯作者：
Li, Ming
作者机构：
[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China.;[Zou, Hong; Zhang, Dong; Wang, Runqiu; Li, Ming; Zhao, Weishan; Wu, Shangong; Wang, Guitang; Li, Wenxiang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.;[Li, Can] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Dong; Wang, Runqiu; Zhao, Weishan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Zheng, Yingzhen] Agr Forestry Anim Husb & Aquaculture Bur Guye Dis, Anim Husb & Aquaculture Stn, Tangshan 063100, Peoples R China.
通讯机构：
[Li, Ming] C;Chinese Acad Sci, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China.;Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
关键词：
Balantidium grimi;ciliate;new species;Quasipaa spinosa;China
摘要：
Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is "V"-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.
语种：
英文
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Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide
作者：
Xu, Xiao;Chen, Shaokui;Wang, Haibo;Tu, Zhixiao;Wang, Shuhui;...
期刊：
British Journal of Nutrition ,2018年119(9):1019-1028 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Xu, Xiao; Liu, Yulan; Chen, Shaokui; Wang, Haibo; Wang, Shuhui; Wang, Chunwei; Tu, Zhixiao; Wang, Xiuying; Zhu, Jiadong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Inflammatory response;Intestine;Medium-chain TAG;Necroptosis;Weanling piglets
摘要：
This study was conducted to evaluate whether medium-chain TAG (MCT) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial inflammatory response, as well as necroptosis. A total of twenty-four weanling piglets were randomly allotted to one of four treatments in a 2×2 factorial arrangement including diet type (5 % maize oil v. 4 % MCT+1 % maize oil) and immune stress (saline v. E. coli LPS). The piglets were fed diets containing maize oil or MCT for 21 d. On 21 d, piglets were injected intraperitoneally with saline or LPS. The blood and intestinal samples were collected at 4 h post injection. Supplementation with MCT improved intestinal morphology, digestive and barrier function, indicated by increased jejunal villus height, increased jejunal and ileal disaccharidases (sucrase and maltase) activities, as well as enhanced protein expression of claudin-1. Furthermore, the protein expression of heat-shock protein 70 in jejunum and the concentration of TNF-α in plasma were reduced in the piglets fed diets supplemented with MCT. In addition, MCT down-regulated the mRNA expression of toll-like receptor 4 (TLR4) and nucleotide-binding oligomerisation domain proteins (NOD) signalling-related genes in jejunum and ileum. Finally, MCT inhibited jejunal and ileal enterocyte necroptosis indicated by suppressed mRNA expression of the receptor-interacting protein 3 and mixed-lineage kinase domain-like protein. These results indicate that MCT supplementation may be closely related to inhibition of TLR4, NOD and necroptosis signalling pathways and concomitant improvement of intestinal integrity under an inflammatory condition. Copyright © The Authors 2018.
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英文
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Transcriptomic analysis reveals differentially expressed genes and a unique apoptosis pathway in channel catfish ovary cells after infection with the channel catfish virus
作者：
Dawar, Farman Ullah;Hu, Xianqin;Zhao, Lijuan;Dong, Xingxing;Xiong, Yang;...
期刊：
Fish & Shellfish Immunology ,2017年71(1):58-68 ISSN：1050-4648
通讯作者：
Mei, Jie;Lin, Li
作者机构：
[Hu, Xianqin; Dong, Xingxing; Xiong, Yang; Mei, Jie; Lin, L; Dawar, Farman Ullah; Lin, Li] Huazhong Agr Univ, Minist Agr, Key Lab Freshwater Anim Breeding,Dept Aquat Anim, Hubei Prov Engn Lab Pond Aquaculture,Coll Fisheri, Wuhan 430070, Hubei, Peoples R China.;[Liang, Rishen; Zhao, Lijuan; Dawar, Farman Ullah; Babu, V. Sarath; Lin, Li; Zhou, Meng] Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangdong Prov Key Lab Waterfowl Hlth Breeding, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Bree, Guangzhou 510225, Guangdong, Peoples R China.;[Hu, Xianqin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.;[Li, Jun] Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA.;[Li, Jun; Lin, Li] Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Shandong, Peoples R China.
通讯机构：
[Mei, J; Lin, L] H;Huazhong Agr Univ, Minist Agr, Key Lab Freshwater Anim Breeding,Dept Aquat Anim, Hubei Prov Engn Lab Pond Aquaculture,Coll Fisheri, Wuhan 430070, Hubei, Peoples R China.
关键词：
Channel catfish;Channel catfish ovary cells;CCV;Transcriptome;DEGs;Apoptosis
摘要：
The channel catfish virus (CCV) can cause lethal hemorrhagic infection in juvenile channel catfish, thereby resulting in a huge economic loss to the fish industry. The genome of the CCV has been fully sequenced, and its prevalence is well documented. However, less is known about the molecular mechanisms and pathogenesis of the CCV. Herein, the channel catfish ovary cells (CCO) were infected with CCV and their transcriptomic sketches were analyzed using an RNA sequencing technique. In total, 72,686,438 clean reads were obtained from 73,231,128 sequence reads, which were further grouped into 747,168 contigs. These contigs were assembled into 49,119 unigenes, of which 20,912 and 18,333 unigenes were found in Nr and SwissProt databases and matched 15,911 and 14,625 distinctive proteins, respectively. From these, 3641 differentially expressed genes (DEGs), comprising 260 up-regulated and 3381 down-regulated genes, were found compared with the control (non-infected) cells. For verification, 16 DEGs were analyzed using qRT-PCR. The analysis of the DEGs and their related cellular signaling pathways revealed a substantial number of DEGs that were involved in the apoptosis pathway induced by CCV infection. The apoptosis pathways were further elucidated using standard apoptosis assays. The results showed that CCV could induce extrinsic apoptosis pathway (instead of a mitochondrial intrinsic apoptosis pathway) in CCO cells. This study helps our understanding of the pathogenesis of CCV and contributes to the prevention of CCV infection in channel catfish. © 2017 Elsevier Ltd
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英文
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Dietary α-ketoglutarate supplementation improves hepatic and intestinal energy status and anti-oxidative capacity of Cherry Valley ducks
作者：
Guo, Shuangshuang;Duan, Rui;Wang, Lei;Hou, Yongqing;Tan, Linglin;...
期刊：
Animal Science Journal ,2017年88(11):1753-1762 ISSN：1344-3941
通讯作者：
Ding, Binying
作者机构：
[Duan, Rui; Cheng, Qiang; Guo, Shuangshuang; Liao, Man; Hou, Yongqing; Tan, Linglin; Wang, Lei; Ding, Binying] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Ding, Binying] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Cherry Valley duck;anti-oxidative capacity;energy status;alpha-ketoglutarate
摘要：
α-Ketoglutarate (AKG) is an extensively used dietary supplement in human and animal nutrition. The aim of the present study was to investigate effects of dietary AKG supplementation on the energy status and anti-oxidative capacity in liver and intestinal mucosa of Cherry Valley ducks. A total of 80 1-day-old ducks were randomly assigned into four groups, in which ducks were fed basal diets supplemented with 0% (control), 0.5%, 1.0% and 1.5% AKG, respectively. Graded doses of AKG supplementation linearly decreased the ratio of adenosine monophosphate (AMP) to adenosine triphosphate (ATP) in the liver, but increased ATP content and adenylate energy charge (AEC) in a quadratic and linear manner, respectively (P < 0.05). Increasing dietary AKG supplemental levels produced linear positive responses in ATP content and AEC, and negative responses in AMP concentration, the ratio of AMP to ATP and total adenine nucleotide in the ileal mucosa (P < 0.05). All levels of dietary AKG reduced the production of jejunal hydrogen peroxide and hepatic malondialdehyde (P < 0.05). Hepatic and ileal messenger RNA expression of AMP kinase α-1 and hypoxia-inducible factor-1α were linearly up-regulated as dietary AKG supplemental levels increased (P < 0.05). In conclusion, dietary AKG supplementation linearly or quadratically enhanced hepatic and intestinal energy storage and anti-oxidative capacity of Cherry Valley ducks.
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英文
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Glutamine and glutaminolysis are required for efficient replication of infectious spleen and kidney necrosis virus in Chinese perch brain cells
作者：
Fu, Xiaozhe;Hu, Xianqin;Li, Ningqiu* ;Zheng, Feifei;Dong, Xingxing;...
期刊：
Oncotarget ,2017年8(2):2400-2412 ISSN：1949-2553
通讯作者：
Li, Ningqiu;Lin, Li
作者机构：
[Li, Ningqiu; Lin, Qiang; Fu, Xiaozhe; Huang, Zhibin] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol,Minist Agr, Key Lab Aquat Anim Immune Technol Guangdong Prov, Guangzhou 510380, Guangdong, Peoples R China.;[Dong, Xingxing; Zhao, Lijuan; Tu, Jiagang; Li, Ningqiu; Lin, Qiang; Duan, Jing; Fu, Xiaozhe; Lin, Li; Hu, Xianqin; Zheng, Feifei; Su, Jianguo; Li, NQ; Lin, L] Huazhong Agr Univ, Coll Fisheries Freshwater Aquaculture Collaborat, Res Ctr Marine Biol, Dept Aquat Anim Med, Wuhan 430070, Hubei, Peoples R China.;[Hu, Xianqin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.;[Fu, Xiaozhe; Su, Jianguo] Northwest A&F Univ, Coll Anim Sci & Technol, Shanxi Key Lab Mol Biol Aquaculture, Yangling 712100, Peoples R China.;[Lin, Li] Hainan Univ, Coll Marine Sci, Hainan Prov Key Lab Trop Hydrobiol & Biotechnol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Peoples R China.
通讯机构：
[Li, Ningqiu] C;[Li, NQ; Lin, L; Lin, Li] H;Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol,Minist Agr, Key Lab Aquat Anim Immune Technol Guangdong Prov, Guangzhou 510380, Guangdong, Peoples R China.;Huazhong Agr Univ, Coll Fisheries Freshwater Aquaculture Collaborat, Res Ctr Marine Biol, Dept Aquat Anim Med, Wuhan 430070, Hubei, Peoples R China.;Hainan Univ, Coll Marine Sci, Hainan Prov Key Lab Trop Hydrobiol & Biotechnol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Peoples R China.
关键词：
siniperca chuatsi;ISKNV;glutamine;glutaminolysis;TCA cycle
摘要：
Viruses rely on host cellular metabolism for energy and macromolecule synthesis during their replication. Infectious spleen and kidney necrosis virus (ISKNV) causes significant economic losses in the Chinese perch (Siniperca chuatsi) industry worldwide. However, little is known about the relationship between ISKNV replication and cellular metabolism. Using transcriptomic analysis, we observed that glutamine metabolism in Chinese perch brain (CPB) cells is altered during ISKNV infection. Moreover, ISKNV replication was decreased in CPB cells cultured in the glutamine-depleted medium. ISKNV replication was also inhibited in CPB cells cultured in the presence of bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (an inhibitor of glutaminase), (-)-epigallocatechinmo nogallate (an inhibitor of glutamate dehydrogenase) or L-buthionine sulfoximine (an inhibitor of glutathione synthesis). However, virus replication was rescued by the addition of multiple tricarboxylic acid cycle intermediates, ATP, or glutathione reduced ethyl ester. ATP and reduced glutathione/oxidized glutathione levels were increased in CPB cells infected with ISKNV, but were decreased in CPB cells cultured in glutamine-depleted medium. These results indicate ISKNV infection induces glutaminolysis to accommodate the biosynthetic and energy needs for its efficient virus replication.
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英文
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Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model
作者：
Zhao, Di;Wu, Tao;Yi, Dan;Wang, Lei;Li, Peng;...
期刊：
International Journal of Molecular Sciences ,2017年18(12) ISSN：1422-0067
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Hou, Yongqing; Wang, Lei; Li, Peng; Wu, Guoyao; Zhao, Di; Yi, Dan; Zhang, Junmei] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Lactobacillus casei;piglets;lipopolysaccharide;liver
摘要：
This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 × 106 cfu/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups receivedintraperitoneal administration of LPS (100 μg/kg body weight), while the control group received the same volume of saline. Blood and liver samples were collected for analysis. Results showed that L. casei supplementation decreased the feed/gain ratio (p = 0.027) and diarrhea incidence (p < 0.001), and attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 0.048), Tumor necrosis factor-alpha (TNF-α) (p = 0.041), and Malondialdehyde (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS also increased (p < 0.05) the mRNA levels for IL-6, IL-8, TNF-α, Toll-like receptors 4 (TLR4), Nuclear factor k B (NF- k B) and Heat shock protein 70 (HSP70) in the liver. The adverse effects of LPS challenge were ameliorated by L. casei supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver injury via reducing pro-inflammatory cytokines and increasing anti-oxidative capacity. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
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英文
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Lanthanum exerts acute toxicity and histopathological changes in gill and liver tissue of rare minnow (Gobiocypris rarus)
作者：
Hua, Dong;Wang, Jianwei;Yu, Denghang;Liu, Jun*
期刊：
Ecotoxicology ,2017年26(9):1207-1215 ISSN：0963-9292
通讯作者：
Liu, Jun
作者机构：
[Yu, Denghang; Hua, Dong; Liu, Jun] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.;[Wang, Jianwei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
通讯机构：
[Liu, Jun] W;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
Toxicity;Gobiocypris rarus;La(III);Histopathological;Gill;Liver
摘要：
We evaluated the acute toxicity effects of lanthanum (La(III)) on gill and liver of rare minnow (Gobiocypris rarus). The median lethal concentration of La (III) at 96 h was 1.92 mg L–1. Rare minnow were reared in freshwater and exposed to 0.04, 0.08, 0.16, 0.32 and 0.80 mg L–1 La (III) for 21 d. Gill and liver samples were analyzed by light microscopy. The main histopathological changes induced by La (III) in gills were epithelial lifting, filamentary epithelial proliferation,edema, lamellar fusion, desquamation, and necrosis. Histopathological changes induced by La (III) in the liver included dilation of sinusoids, focal congestion, pyknotic nuclei, karyohexis and karyolysis, vacuolar degeneration, and numerous necrosis areas. Hypsometric analysis indicated significant changes in the measures of gill dimensions (average length, width, area), suggesting metabolic disturbance (gas exchange) upon La (III) exposure. The result showed that La (III) severely affects fish gill and liver. © 2017, Springer Science+Business Media, LLC.
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英文
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Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing
作者：
Sun, Zongyi;Jiang, Chuanqi;Feng, Jinmei;Yang, Wentao;Li, Ming* ;...
期刊：
PARASITE ,2017年24:43 ISSN：1252-607X
通讯作者：
Li, Ming;Miao, Wei
作者机构：
[Li, Ming; Sun, Zongyi; Li, M; Miao, W] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Li, Ming; Yang, Wentao; Li, M; Miao, W; Jiang, Chuanqi; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.;[Feng, Jinmei] Jianghan Univ, Sch Med, Dept Pathogen Biol, Wuhan 430056, Hubei, Peoples R China.
通讯机构：
[Li, M; Miao, W] W;[Li, M; Miao, W] C;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
关键词：
Balantidium ctenopharyngodoni;Trichostomatia;single-cell transcriptome sequencing;SSU rDNA;phylogenomic analysis
摘要：
In this paper, we present transcriptome data for Balantidium ctenopharyngodoni Chen, 1955 collected from the hindgut of grass carp (Ctenopharyngodon idella). We evaluated sequence quality and de novo assembled a preliminary transcriptome, including 43.3 megabits and 119,141 transcripts. Then we obtained a final transcriptome, including 17.7 megabits and 35,560 transcripts, by removing contaminative and redundant sequences. Phylogenomic analysis based on a supermatrix with 132 genes comprising 53,873 amino acid residues and phylogenetic analysis based on SSU rDNA of 27 species were carried out herein to reveal the evolutionary relationships among six ciliate groups: Colpodea, Oligohymenophorea, Litostomatea, Spirotrichea, Heterotrichea and Protocruziida. The topologies of both phylogenomic and phylogenetic trees are discussed in this paper. In addition, our results suggest that single-cell sequencing is a sound method of obtaining sufficient omics data for phylogenomic analysis, which is a good choice for uncultivable ciliates. The transcriptome data for Balantidium ctenopharyngodoni are the first omics data within the subclass Trichostomatia, and provide a good basis for ciliate phylogenomic analysis, as well as related omics analysis.
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英文
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Asparagine preserves intestinal barrier function from LPS-induced injury and regulates CRF/CRFR signaling pathway
作者：
Zhu, Huiling;Pi, Dingan;Leng, Weibo;Wang, Xiuying;Hu, Chien-An Andy;...
期刊：
INNATE IMMUNITY ,2017年23(6):546-556 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Hu, Chien-An Andy; Qin, Qin; Liu, Yulan; Leng, Weibo; Xiong, Jianglin; Hou, Yongqing; Wang, Chunwei; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Liu, Yulan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Collaborat Innovat Ctr Anim Nutr & Feed Safety, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Collaborat Innovat Ctr Anim Nutr & Feed Safety, Wuhan 430023, Hubei, Peoples R China.
关键词：
*Asparagine;*CRF/CRFR1 signaling pathway;*intestinal barrier function;*lipopolysaccharide;*weaned pigs
摘要：
Stress causes intestinal inflammation and barrier dysfunction. Corticotrophin-releasing factor (CRF)/CRF receptor (CRFR) signaling pathway has been shown to be important for stress-induced intestinal mucosal alteration. L-Asparagine (ASN) is a powerful stimulator of ornithine decarboxylase and cell proliferation in a variety of cell types, including colonic cells. In the present study, we investigated whether dietary ASN supplementation could alleviate the damage of intestinal barrier function caused by LPS through modulation of CRF/CRFR signaling pathway. Twenty-four weaned pigs were randomly divided into one of four treatments: (1) non-challenged control; (2) Escherichia coli LPS challenged control; (3) LPS + 0.5% ASN; (4) LPS + 1.0% ASN. LPS stress induced villous atrophy, intestinal morphology disruption and decreased claudin-1 expression. ASN supplementation increased intestinal claudin-1 protein expression and alleviated villous atrophy and intestinal morphology impairment caused by LPS stress. In addition, ASN supplementation increased the number of intestinal intraepithelial lymphocytes and reversed the elevations of intestinal mast cell number and neutrophil number induced by LPS stress. Moreover, ASN decreased the mRNA expression of intestinal CRF, glucocorticoid receptors and tryptase. These results indicate that ASN attenuates intestinal barrier dysfunction induced by LPS stress, and regulates CRF/CRFR1 signaling pathway and mast cell activation. © The Author(s) 2017.
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英文
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N-Acetylcysteine supplementation alleviates intestinal injury in piglets infected by porcine epidemic diarrhea virus
作者：
Wang, Lei;Zhou, Jia;Hou, Yongqing* ;Yi, Dan;Ding, Binying;...
期刊：
Amino Acids ,2017年49(12):1931-1943 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Wu, Tao; Hu, Chien-An Andy; Zhang, Yue; Zhou, Jia; Hou, Yongqing; Wang, Lei; Xie, Jiaqian; Wu, Guoyao; Ding, Binying; Zhao, Di; Yi, Dan; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
N-Acetylcysteine;Intestinal functions;Piglets;Porcine epidemic diarrhea virus
摘要：
Porcine epidemic diarrhea virus (PEDV) infects the intestine of young pigs, but effective measures for prevention and treatment are lacking. N-Acetylcysteine (NAC) has been shown to reduce endotoxin-induced intestinal dysfunction. This study was conducted with the PEDV-infected neonatal piglet model to determine the effect of NAC supplementation on intestinal function. Thirty-two 7-day-old piglets were randomly allocated to one of four treatments in a 2 × 2 factorial design consisting of two liquid diets (0 or 50 mg/kg BW NAC supplementation) and oral administration of 0 or 104.5 TCID50 (50% tissue culture infectious dose) PEDV. On day 7 of the trial, half of the pigs (n = 8) in each dietary treatment received either sterile saline or PEDV (Yunnan province strain) solution at 104.5 TCID50 per pig. On day 10 of the trial, d-xylose (0.1 g/kg BW) was orally administrated to all pigs. One hour later, jugular vein blood samples were collected, and then all pigs were killed to obtain the small intestine. PEDV infection increased diarrhea incidence, while reducing ADG. PEDV infection also decreased plasma d-xylose concentration, small intestinal villus height, mucosal I-FABP and villin mRNA levels but increased mucosal MX1 and GCNT3 mRNA levels (P < 0.05). Dietary NAC supplementation ameliorated the PEDV-induced abnormal changes in all the measured variables. Moreover, NAC reduced oxidative stress, as indicated by decreases in plasma and mucosal H2O2 levels. Collectively, these novel results indicate that dietary supplementation with NAC alleviates intestinal mucosal damage and improves the absorptive function of the small intestine in PEDV-infected piglets. © 2017, Springer-Verlag Wien.
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Roles of amino acids in preventing and treating intestinal diseases: recent studies with pig models
作者：
Liu, Yulan* ;Wang, Xiuying;Hou, Yongqing;Yin, Yulong;Qiu, Yinsheng;...
期刊：
Amino Acids ,2017年49(8):1277-1291 ISSN：0939-4451
通讯作者：
Liu, Yulan;Hu, Chien-An Andy
作者机构：
[Hu, Chien-An Andy; Liu, Yulan; Hou, Yongqing; Qiu, Yinsheng; Liu, YL; Hu, CAA; Wang, Xiuying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Hu, Chien-An Andy; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr & Hlth, Changsha 410125, Hunan, Peoples R China.;[Hu, Chien-An Andy; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol, Changsha 410125, Hunan, Peoples R China.;[Hu, Chien-An Andy; Yin, Yulong] Human Normal Univ, Inst Life Sci, Changsha 410081, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Liu, YL; Hu, CAA] W;[Hu, Chien-An Andy] C;[Hu, Chien-An Andy] H;[Hu, Chien-An Andy] U;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
Pig models;Intestinal disease;Amino acids;Therapeutics
摘要：
Animal models are needed to study and understand a human complex disease. Because of their similarities in anatomy, structure, physiology, and pathophysiology, the pig has proven its usefulness in studying human gastrointestinal diseases, such as inflammatory bowel disease, ischemia/reperfusion injury, diarrhea, and cancer. To understand the pathogenesis of these diseases, a number of experimental models generated in pigs are available, for example, through surgical manipulation, chemical induction, microbial infection, and genetic engineering. Our interests have been using amino acids as therapeutics in pig and human disease models. Amino acids not only play an important role in protein biosynthesis, but also exert significant physiological effects in regulating immunity, anti-oxidation, redox regulation, energy metabolism, signal transduction, and animal behavior. Recent studies in pigs have shown that specific dietary amino acids can improve intestinal integrity and function under normal and pathological conditions that protect the host from different diseases. In this review, we summarize several pig models in intestinal diseases and how amino acids can be used as therapeutics in treating pig and human diseases.
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英文
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Dietary supplementation of branched-chain amino acids increases muscle net amino acid fluxes through elevating their substrate availability and intramuscular catabolism in young pigs
作者：
Zheng, Liufeng;Zuo, Fangrui;Zhao, Shengjun;He, Pingli;Wei, Hongkui;...
期刊：
British Journal of Nutrition ,2017年117(7):911-922 ISSN：0007-1145
通讯作者：
Peng, Jian
作者机构：
[Zheng, Liufeng; Zuo, Fangrui; Wei, Hongkui; Xiang, Quanhang; Pang, Jiaman; Peng, Jian] Huazhong Agr Univ, Dept Anim Nutr & Feed Sci, Coll Anim Sci & Technol, Wuhan 430070, Peoples R China.;[Zhao, Shengjun] Wuhan Polytech Univ, Dept Feed Sci, Wuhan 430070, Peoples R China.;[He, Pingli] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Wei, Hongkui; Peng, Jian] Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Peoples R China.
通讯机构：
[Peng, Jian] H;[Peng, Jian] C;Huazhong Agr Univ, Dept Anim Nutr & Feed Sci, Coll Anim Sci & Technol, Wuhan 430070, Peoples R China.;Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Peoples R China.
关键词：
Branched-chain amino acids;Young pigs;Hindlimb muscle;Net amino acid fluxes;Metabolomic profiling
摘要：
Branched-chain amino acids (BCAA) have been clearly demonstrated to have anabolic effects on muscle protein synthesis. However, little is known about their roles in the regulation of net AA fluxes across skeletal muscle in vivo. This study was aimed to investigate the effect and related mechanisms of dietary supplementation of BCAA on muscle net amino acid (AA) fluxes using the hindlimb flux model. In all fourteen 4-week-old barrows were fed reduced-protein diets with or without supplemental BCAA for 28 d. Pigs were implanted with carotid arterial, femoral arterial and venous catheters, and fed once hourly with intraarterial infusion of p-amino hippurate. Arterial and venous plasma and muscle samples were obtained for the measurement of AA, branched-chain α-keto acids (BCKA) and 3-methylhistidine (3-MH). Metabolomes of venous plasma were determined by HPLC-quadrupole time-of-flight-MS. BCAA-supplemented group showed elevated muscle net fluxes of total essential AA, non-essential AA and AA. As for individual AA, muscle net fluxes of each BCAA and their metabolites (alanine, glutamate and glutamine), along with those of histidine, methionine and several functional non-essential AA (glycine, proline and serine), were increased by BCAA supplementation. The elevated muscle net AA fluxes were associated with the increase in arterial and intramuscular concentrations of BCAA and venous metabolites including BCKA and free fatty acids, and were also related to the decrease in the intramuscular concentration of 3-MH. Correlation analysis indicated that muscle net AA fluxes are highly and positively correlated with arterial BCAA concentrations and muscle net BCKA production. In conclusion, supplementing BCAA to reduced-protein diet increases the arterial concentrations and intramuscular catabolism of BCAA, both of which would contribute to an increase of muscle net AA fluxes in young pigs. © The Authors 2017.
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英文
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Evaluation of recombinant protein superoxide dismutase of Haemophilus parasuis strain SH0165 as vaccine candidate in a mouse model
作者：
Guo, Ling;Xu, Lei;Wu, Tao;Fu, Shulin* ;Qiu, Yinsheng* ;...
期刊：
CANADIAN JOURNAL OF MICROBIOLOGY ,2017年63(4):312-320 ISSN：0008-4166
通讯作者：
Fu, Shulin;Qiu, Yinsheng
作者机构：
[Wu, Tao; Hu, Chien-An Andy; Xu, Lei; Qiu, Yinsheng; Fu, Shulin; Guo, Ling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Ren, Xinglong] Huazhong Agr Univ, Coll Sci, Wuhan 430070, Peoples R China.;[Ye, Mengdie; Liu, Rongrong] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Fu, SL; Qiu, YS] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Haemophilus parasuis;Glässer’s disease;superoxide dismutase;vaccine;Haemophilus parasuis;maladie de Glässer;superoxyde dismutase;vaccin
摘要：
Haemophilus parasuis can cause a severe membrane inflammation disorder. It has been documented that superoxide dismutase (SOD) is a potential target to treat systemic inflammatory diseases. Therefore, we constructed an experimental H. parasuis subunit vaccine SOD and determined the protective efficacy of SOD using a lethal dose challenge against H. parasuis serovar 4 strain MD0322 and serovar 5 strain SH0165 in a mouse model. The results demonstrated that SOD could induce a strong humoral immune response in mice and provide significant immunoprotection efficacy against a lethal dose of H. parasuis serovar 4 strain MD0322 or serovar 5 strain SH0165 challenge. IgG subtype analysis indicated SOD protein could trigger a bias toward a Th1-type immune response and induce the proliferation of splenocytes and secretion of IL-2 and IFN-γ of splenocytes. In addition, serum in mice from the SOD-immunized group could inhibit the growth of strain MD0322 and strain SH0165 in the whole-blood killing bacteria assay. This is the first report that immunization of mice with SOD protein could provide protective effect against a lethal dose of H. parasuis serovar 4 and serovar 5 challenge in mice, which may provide a novel approach against heterogeneous serovar infection of H. parasuis in future. © 2017, Canadian Science Publishing. All rights reserved.
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英文
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Response of Selenium and Selenogenome in Immune Tissues to LPS-Induced Inflammatory Reactions in Pigs
作者：
Sun, Lv-Hui;Pi, Ding-An;Zhao, Ling;Wang, Xiu-Ying;Zhu, Luo-Yi;...
期刊：
Biological Trace Element Research ,2017年177(1):90-96 ISSN：0163-4984
通讯作者：
Liu, Yu-Lan
作者机构：
[Zhu, Luo-Yi; Zhao, Ling; Sun, Lv-Hui; Qi, De-Sheng] Huazhong Agr Univ, Dept Anim Nutr & Feed Sci, Coll Anim Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Wang, Xiu-Ying; Pi, Ding-An; Liu, Yu-Lan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
通讯机构：
[Liu, Yu-Lan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
关键词：
Selenium;Selenoprotein;LPS;Inflammatory reaction;Pigs
摘要：
Circulating concentration of the essential trace element selenium (Se) was significantly lower in inflammatory disorders. Although Se plays physiological roles mainly through the function of 25 selenoproteins, the response of the selenogenome in immune tissues during inflammatory reactions remains unclear. The objective of this study was to determine the Se retention and selenogenome expression in immune tissues during the lipopolysaccharide (LPS)-induced inflammatory response in porcine. A total of 12 male pigs were randomly divided into two groups and injected with LPS or saline. After 4 h postinjection, blood samples were collected and pigs were euthanized. Pigs challenged with LPS had 36.8 and 16.6 % lower (P < 0.05) Se concentrations in the serum and spleen, respectively, than those injected with saline. Moreover, the activities of GPX decreased (P < 0.05) by 23.4, 26.6, and 30.4 % in the serum, thymus, and lymph node, respectively, in the pigs injected with LPS. Furthermore, the LPS challenge altered (P < 0.05) the mRNA expression of 14, 16, 10, and 6 selenoprotein genes in the liver, spleen, thymus, and lymph node, respectively. Along with 10 previously reported selenoprotein genes, the response of Txnrd2, Txnrd3, Sep15, Selh, Seli, Seln, Selo, Selt, Selx, and Sephs2 to inflammatory reaction in immune tissues were newly illustrated in this study. In conclusion, the LPS-induced inflammatory response impaired Se metabolism and was associated with dysregulation of the selenogenome expression in immune tissues.
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英文
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Nutritionally Nonessential Amino Acids: A Misnomer in Nutritional Sciences
作者：
Hou, Yongqing;Wu, Guoyao
期刊：
Advances in Nutrition ,2017年8(1):137-139 ISSN：2161-8313
通讯作者：
Wu, G.
作者机构：
[Hou, Yongqing] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, China;[Wu, Guoyao] Departments of Animal Science and Medical Physiology, Texas A&M University, College Station, TX g-wu@tamu.edu
通讯机构：
Departments of Animal Science and Medical Physiology, Texas A and M University, College Station, TX, United States
摘要：
Amino acids (AAs)3 are organic compounds that contain amino and acid groups (1). Based on the configuration of glyceraldehyde (l- or d-isomers as introduced by Emil Fischer in 1908), AAs (except for Gly, taurine, β-alanine, and γ-aminobutyrate, which have no asymmetric carbon) exist as either l- or d-AAs. l-AAs are much more abundant than d-AAs in nature and are the physiologic isomers in animal and plant proteins. The AAs whose carbon skeletons are not synthesized de novo by animal cells were termed “nutritionally essential” AAs (EAAs) in 1912 and must be provided to animals to maintain their growth or nitrogen balance (2). In all animals, the EAAs consist of His, Ile, Leu, Lys, Met, Phe, Thr, Trp, and Val (3). In contrast, AAs whose carbon skeletons are synthesized de novo by animal cells were considered to be dispensable in diets and were classified as “nutritionally nonessential” AAs (NEAAs) (2). In most mammals (e.g., humans, rats, and pigs), the traditionally classified NEAAs are Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, Pro, Ser, and Tyr (3). The concepts of EAAs and NEAAs have been used for more than a century. Increasing evidence from studies in pigs, poultry, and fish has shown that animals do have dietary requirements of NEAAs to fulfill their genetic potential for maximum growth, reproduction, lactation, and production performance, as well as optimal health and well-being (4, 5).
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