期刊：
CURRENT MEDICINAL CHEMISTRY,2025年32:1-13 ISSN：0929-8673
作者机构：
[Wenbin Liu] College of Medicine and Health Science, Wuhan Polytechnic
University, Wuhan, 430023, China;[Muhammad Umer] Research Center of Forest
Ecology, Institute for Forest Resources and Environment of Guizhou and Forestry College, Guizhou University,
Guiyang, 550025, China;[Yang Xu] Wuhan Polytechnic University School of Modern Industry for Selenium Science and Engineering Wuhan China;[Jixin Chen; Yi He; Shiya Wei; Zhangqian Wang; Chao Gao] National R&[Jixin Chen; Yi He; Shiya Wei; Zhangqian Wang; Chao Gao] D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center
for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science
and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
Introduction: G-quadruplexes (G4s) are non-classical high-level structures that are formed by DNA/RNA sequences and have been a promising target for developing antitumor drugs. However, it is still a challenge to find a ligand that binds to a particular G4 with selectivity. Telomeric multimeric G4s are more accessible for screening for specific ligands due to their higher-order structure compared with telomeric monomeric G4s.<&wdkj&>Methods : In this study, the natural product berberine was found to exhibit a higher selectivity for telomeric multimeric G4 in comparison with other G4s. The mechanism of interaction between telomeric G4s and berberine was further investigated by fluorescence spectra measurements, job plot analysis, and UV titrations. We found that there are three binding sites for berberine on telomeric dimeric G-quadruplex Tel45, which are located at the 5' and 3' terminal G-quartet surfaces and the pocket between the two quadruplex units of Tel45. It was worth noting that the berberine preferred to interact within the interfacial cavity between two G4 units.<&wdkj&>Results : Moreover, via dynamic light scattering (DLS) and native polyacrylamide gel electrophoresis (Native-PAGE) assays, it was found that the particle size of the telomeric multimeric G4s conformation was significantly increased by the addition of berberine. In contrast, the particle sizes of Tel21 did not change significantly after the addition of berberine. An immunofluorescence assay indicated that berberine induced the formation of endogenous telomeric G4 structures along with the related telomeric DNA damage response.<&wdkj&>Conclusion: This study provides a hypothetical basis for the development of natural products targeting telomeric G4 as antitumor drugs.
作者机构：
[Fen Teng] Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Longxia Hu; Jiaqing Wang; Yi Dai; Lihong Bao; Yun Yang; Tingting Jiao; Fei Luo; Yali Yang; Zixi Wang; Xiaoping Yang; Shengli Yang] Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Deying Hu; Yilan Liu; Yanhong Han; Xiaoping Ding] Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;[Wenbin Liu; Hongling Zhang; Qingzhou Cheng] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Deying Hu] D;[Wenbin Liu] C;Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
关键词：
attitude;knowledge;living will;malignant tumor;palliative care
摘要：
Given the suboptimal quality of end-of-life care among patients with cancer in China, promoting living wills is critical in this population. Living wills ensure that individuals can receive the medical care they desire during the terminal phase of an illness, maintain their dignity, and ultimately achieve a good death. However, current awareness and attitudes about living wills among Chinese patients with cancer remain unclear. We administered a questionnaire survey on living wills to patients with malignant tumors to assess their most important needs and increase understanding about living wills.
In this cross-sectional study using convenience sampling, inpatients with malignant tumors in Wuhan completed our questionnaire between July 2020 and June 2021. We collected patients' sociodemographic characteristics and details regarding their knowledge and attitudes about living wills.
Among 213 patients with malignant tumors, 114 (53.52%) had heard of living wills; 125 (58.69%) expressed their willingness to sign the “Five Wishes” living will document after learning about it through the questionnaire. Patients with malignant tumors had a high level of desire for the following living will items: the lives of family and friends return to normal as soon as possible after their death, maintaining personal hygiene and dignity, and remaining pain-free. The knowledge level of patients with malignant tumors was related to their educational level ( p < 0.05) and self-care ability ( p < 0.05).
Patients with malignant tumors have a high need for comfort, cleanliness, and pain relief in the terminal stages. Patients with a higher level of education and those with poorer self-care ability had greater knowledge and acceptance of living wills. Promotion can first be targeted toward more highly educated patients and can then be gradually expanded to other groups.
期刊：
FRONTIERS IN PSYCHIATRY,2025年16:1588065 ISSN：1664-0640
作者机构：
[Yihan, He; Luo, Jiyu; Xu, Ao; Qiu, Dongmei; Huang, Haiyun; Peng, Xiaorui; Zhou, Yujia; Hu, Xiang; Zhang, Hongling] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, Hubei Province, China;[Zhou, Hui] Huangshi Hospital of Traditional Chinese Medicine, Huangshi, China;[Xu, Lingyun; Li, Yang] School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei Province, China;[Li, Yuanyuan] Key Laboratory for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hebei Province, China
关键词：
family functioning;stressful life events;Second trimester of pregnancy;Depression;Mediation
摘要：
Background: In current research on prenatal depression among pregnant women, the role of family functioning is crucial yet often overlooked. Specifically, in the field of maternal mental health, relatively limited attention has been given to the psychological well-being of women during the second trimester of pregnancy. This study aims to assess the depression, family functioning, and stressful life events of women in their second trimester, and to explore whether family functioning mediates the relationship between stressful events and depressive symptoms. Methods: A questionnaire survey was conducted with 3,386 pregnant women from the Wuhan Healthy Baby Birth Cohort. Participants completed the 10-item Center for Epidemiologic Studies Depression Scale (CESD-10), the Family APGAR Index, and the 18-item Stressful Life Events Scale. Structural equation modeling was applied to analyze the data. Results: The findings revealed significant interrelationships among depression, family functioning, and stressful life events. Family functioning was found to partially mediate the relationship between stressful life events and depressive symptoms during the second trimester, explaining 21.9% of the variance. Conclusions: These findings suggest that improving family functioning and fostering communication can help reduce depressive symptoms during the second trimester, supporting maternal mental health.
摘要：
Blended vegetable oil is considered to be a valuable product in the market owing to favourable taste and nutritional composition. The quantification of its contents has notable implications for protecting food safety and consumer interests. Thus, a rapid and non-destructive method is needed to analyse the composition of blended oil. This study established an analytical method combining Raman spectroscopy and prediction models to determine the content of olive oil in a mixture. Competitive adaptive reweighted sampling was employed to select feature bands attributed to β-carotene and unsaturated fatty acids. Various models were used to calculate the mixture proportion, and the importance of characteristic peak intensity affecting the prediction was evaluated via grey relational analysis. The random forest model exhibited superior performance in quantitative analysis, with RMSE and R 2 of 0.0447 and 0.9799, respectively. Overall, this approach was proven to effectively identify blended olive oils, exemplifying its potential in food authentication.
Blended vegetable oil is considered to be a valuable product in the market owing to favourable taste and nutritional composition. The quantification of its contents has notable implications for protecting food safety and consumer interests. Thus, a rapid and non-destructive method is needed to analyse the composition of blended oil. This study established an analytical method combining Raman spectroscopy and prediction models to determine the content of olive oil in a mixture. Competitive adaptive reweighted sampling was employed to select feature bands attributed to β-carotene and unsaturated fatty acids. Various models were used to calculate the mixture proportion, and the importance of characteristic peak intensity affecting the prediction was evaluated via grey relational analysis. The random forest model exhibited superior performance in quantitative analysis, with RMSE and R 2 of 0.0447 and 0.9799, respectively. Overall, this approach was proven to effectively identify blended olive oils, exemplifying its potential in food authentication.
期刊：
Neural Computing and Applications,2025年37(6):5169-5186 ISSN：0941-0643
通讯作者：
Shan Zeng
作者机构：
[Cheng Zhang; Shan Zeng; Zhiguang Yang; Hao Li] College of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, China;[Yulong Chen] College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China;[Yuanyan Tang] Faculty of Science and Technology, University of Macau, Macau, China
通讯机构：
[Shan Zeng] C;College of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, China
摘要：
Intelligent transportation system (ITS) plays an important role in assisting drivers to master road information and optimize traffic flow. However, image degradation resulting from complicated environmental factors, such as motion blur caused by vehicle movement and illumination condition, caused some difficulties in current object recognition research on the ITS that may pose serious risks to driving safety. In order to tackle these challenges, this paper introduces a novel perturbation-based image super-resolution method based on GAN inversion (PSRGANI), utilizing a perturbation mechanism to better assist the latent space escape from the local optimum. In the architecture of PSRGANI, a dual encoder that preserves high-dimensional semantic feature from degraded images, extracts texture information from high-resolution (HR) images and completes the SR reconstruction process via perturbation mechanism. The additional encoder effectively decreases illuminated interference from external environment, enhancing the result robustness of SR reconstruction. A dual discriminator precisely regulates the upsampling process of the decoder for improving the quality of generated images. The additional discriminator achieves the optimal fusion of inputs from different sources by decoupling. SR experiment results reveal the higher evaluation metrics of PSRGANI in texture and decoupling compared with other SR models such as ESRGAN and DGP. In real-world ITS of traffic sign experiments, PSRGANI-applied model shows better performance (Top-1 Class Error of 3%) and faster inference speed (0.06 s per image) when compared to target detection algorithms such as YOLO and DETR. PSRGANI is demonstrated to have accurate results on degraded image recognition in terms of texture quality and evaluation metrics.
摘要：
This study investigated the antiepileptic effects of polydatin (PD) and its potential mechanisms. Racine scoring and EEG were used to assess seizure severity in a PTZ-induced mouse epilepsy model. The results demonstrated that PD significantly reduced the intensity of seizures. Behavioral tests revealed that PD improved learning, memory, and motor coordination. Histological analysis using HE and Nissl staining showed that PD alleviated hippocampal neuronal damage. TUNEL staining further confirmed that PD effectively prevented neuronal apoptosis. Network pharmacology predicted SIRT1 as a potential target of PD, and GO and KEGG pathway enrichment analyses indicated that PD regulated mitochondrial function. Therefore, PD may reduce neuronal damage by protecting mitochondrial function through SIRT1. This process was tested both in vitro and in vivo . In a Mg 2+ -free neuronal excitability model, CCK-8, LDH, and Hoechst staining results demonstrated that PD significantly increased cell survival and reduced apoptosis. Reduced mitosox staining, along with increased activity of Complexes IV and V and ATP production, indicated that PD mitigated mitochondrial function. Gene expression studies revealed that PD activated the SIRT1-PGC-1α signaling pathway via AMPK. In conclusion, PD exerted neuroprotective effects by regulating mitochondrial function, suggesting a potential therapeutic target for epilepsy.
This study investigated the antiepileptic effects of polydatin (PD) and its potential mechanisms. Racine scoring and EEG were used to assess seizure severity in a PTZ-induced mouse epilepsy model. The results demonstrated that PD significantly reduced the intensity of seizures. Behavioral tests revealed that PD improved learning, memory, and motor coordination. Histological analysis using HE and Nissl staining showed that PD alleviated hippocampal neuronal damage. TUNEL staining further confirmed that PD effectively prevented neuronal apoptosis. Network pharmacology predicted SIRT1 as a potential target of PD, and GO and KEGG pathway enrichment analyses indicated that PD regulated mitochondrial function. Therefore, PD may reduce neuronal damage by protecting mitochondrial function through SIRT1. This process was tested both in vitro and in vivo . In a Mg 2+ -free neuronal excitability model, CCK-8, LDH, and Hoechst staining results demonstrated that PD significantly increased cell survival and reduced apoptosis. Reduced mitosox staining, along with increased activity of Complexes IV and V and ATP production, indicated that PD mitigated mitochondrial function. Gene expression studies revealed that PD activated the SIRT1-PGC-1α signaling pathway via AMPK. In conclusion, PD exerted neuroprotective effects by regulating mitochondrial function, suggesting a potential therapeutic target for epilepsy.
摘要：
Sebum composition may be more important than amount for acne lesions, and current research on skin surface lipids (SSLs) focuses on determining their relative content. The objective of this study was to analyze the changes in the absolute content of SSLs in acne patients and their relationship with skin barrier function. To evaluate skin barrier function, transepidermal water loss (TEWL), skin moisture, sebum content, skin elasticity, and whiteness were measured, while SSL changes were investigated using LC-MS/MS. The results indicated that adult acne patients have reduced skin barrier function, as demonstrated by changes in skin moisture, sebum content, skin flexibility, and whitening. Notably, AGlcSiE, Cer, CL, Co, LPC, PA, PC, PE, PI, SM, So, SQDG, and TG were considerably enhanced in acne patients' SSLs, whereas CerG1, DG, DGDG, MGDG, PG, and phSM were decreased. Furthermore, side chain analysis showed that the ratio of linoleic acid to linolenic acid in acne patients' skin surface lipids was higher than in healthy controls, and the caprylic acid/capric acid ratio was likewise greater. The correlation study of SSLs and skin barrier function demonstrated that increasing LPC and decreasing PG are associated with skin barrier function deterioration. In conclusion, acne patients have compromised skin barrier function and altered SSL absolute content, and certain SSL species identified in this study could serve as potential targets for research into acne pathogenesis.
摘要：
Both colchicine (Col) and loxoprofen sodium (LS) are first-line drugs for gouty arthritis (GA). Dissolvable microneedles (DMNs) represent an innovative transdermal drug delivery system (TDDS) that maximizes the therapeutic effect of drugs. In this paper, a novel TDDS of combined Col with LS loaded in DMNs (Col-LS-DMNS) was prepared to treat GA. To assess the synergistic effects of Col and LS, cell viability assays were conducted using monosodium urate (MSU)-induced HaCaT cells. The optimization of the Col-LS-DMNs formulation was carried out through sealing membrane puncture tests, single-factor experiments, and Box-Behnken response surface methodology. The quality of Col-LS-DMNs was assessed using high-performance liquid chromatography (HPLC), scanning electron microscopy (SEM), sealing membrane puncture tests, in vitro mouse skin puncture experiments, and Franz diffusion cell assays. The therapeutic effect of Col-LS DMNs on GA through animal experiments. Results demonstrated that Col and LS exhibit a synergistic effect, with optimal mass ratio of 1:1. The optimal formulation included PVP K30 and CMC Na at a mass ratio of 10:1, with 63 % water content and a mixed dosage of Col and LS of 42 mg. The drug content in Col-LS-DMNs was (19.11 ± 0.65) μg of Col and (20.69 ± 0.89) μg of LS per tablet. SEM imaging revealed that the needle tips were conical. Additionally, Col-LS-DMNs exhibited favorable mechanical properties and caused minimal skin damage. After 24 h, the cumulative permeation amount was (21.55 ± 19.49) μg/cm2. Col-LS-DMNs significantly reduced MSU-induced ankle swelling and decreased serum concentrations of TNF-α, IL-6, and IL-1β in rats. In conclusion, Col-LS-DMNs represent a promising new treatment modality for GA.
Both colchicine (Col) and loxoprofen sodium (LS) are first-line drugs for gouty arthritis (GA). Dissolvable microneedles (DMNs) represent an innovative transdermal drug delivery system (TDDS) that maximizes the therapeutic effect of drugs. In this paper, a novel TDDS of combined Col with LS loaded in DMNs (Col-LS-DMNS) was prepared to treat GA. To assess the synergistic effects of Col and LS, cell viability assays were conducted using monosodium urate (MSU)-induced HaCaT cells. The optimization of the Col-LS-DMNs formulation was carried out through sealing membrane puncture tests, single-factor experiments, and Box-Behnken response surface methodology. The quality of Col-LS-DMNs was assessed using high-performance liquid chromatography (HPLC), scanning electron microscopy (SEM), sealing membrane puncture tests, in vitro mouse skin puncture experiments, and Franz diffusion cell assays. The therapeutic effect of Col-LS DMNs on GA through animal experiments. Results demonstrated that Col and LS exhibit a synergistic effect, with optimal mass ratio of 1:1. The optimal formulation included PVP K30 and CMC Na at a mass ratio of 10:1, with 63 % water content and a mixed dosage of Col and LS of 42 mg. The drug content in Col-LS-DMNs was (19.11 ± 0.65) μg of Col and (20.69 ± 0.89) μg of LS per tablet. SEM imaging revealed that the needle tips were conical. Additionally, Col-LS-DMNs exhibited favorable mechanical properties and caused minimal skin damage. After 24 h, the cumulative permeation amount was (21.55 ± 19.49) μg/cm2. Col-LS-DMNs significantly reduced MSU-induced ankle swelling and decreased serum concentrations of TNF-α, IL-6, and IL-1β in rats. In conclusion, Col-LS-DMNs represent a promising new treatment modality for GA.
期刊：
Saudi Pharmaceutical Journal,2024年32(6):102100 ISSN：1319-0164
通讯作者：
Xu, LY;Qiu, YS
作者机构：
[Li, Junjie; Jiang, Peipei; Xu, Lingyun; Han, Di; Liu, Mingxue; Wang, Yingzhou] Wuhan Polytech Univ, Sch Life Sci & Technol, Xuefu South Rd 68,Changqing Garden, Wuhan 430023, Peoples R China.;[Zhang, Hongling] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan 430023, Peoples R China.;[Qiu, Yinsheng] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Xuefu South Rd 68,Changqing Garden, Wuhan 430023, Peoples R China.
通讯机构：
[Xu, LY ; Qiu, YS ] W;Wuhan Polytech Univ, Sch Life Sci & Technol, Xuefu South Rd 68,Changqing Garden, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Xuefu South Rd 68,Changqing Garden, Wuhan 430023, Peoples R China.
关键词：
Baicalin;Microemulsion-based gel;Skin penetration and retention;Gouty arthritis;Analgesic and anti-inflammatory activity
摘要：
We previously demonstrated that baicalin had efficacy against gouty arthritis (GA) by oral administration. In this paper, a novel baicalin-loaded microemulsion-based gel (B-MEG) was prepared and assessed for the transdermal delivery of baicalin against GA. The preparation method and transdermal capability of B-MEG was screened and optimized using the central composite design, Franz diffusion cell experiments, and the split-split plot design. Skin irritation tests were performed in guinea pigs. The anti-gout effects were evaluated using mice. The optimized B-MEG comprised of 50 % pH 7.4 phosphate buffered saline, 4.48 % ethyl oleate, 31.64 % tween 80, 13.88 % glycerin, 2 % borneol, 0.5 % clove oil and 0.5 % xanthan gum, with a baicalin content of (10.42 ± 0.08) mg/g and particle size of (15.71 ± 0.41) nm. After 12 h, the cumulative amount of baicalin permeated from B-MEG was (672.14 ± 44.11) μg·cm−2. No significant skin irritation was observed following B-MEG application. Compared to the model group, B-MEG groups significantly decreased the rate of auricular swelling (P < 0.01) and number of twists observed in mice (P < 0.01); and also reduced the rate of paw swelling (P < 0.01) and inflammatory cell infiltration in a mouse model of GA. In conclusion, B-MEG represents a promising transdermal carrier for baicalin delivery and can be used as a potential therapy for GA.
We previously demonstrated that baicalin had efficacy against gouty arthritis (GA) by oral administration. In this paper, a novel baicalin-loaded microemulsion-based gel (B-MEG) was prepared and assessed for the transdermal delivery of baicalin against GA. The preparation method and transdermal capability of B-MEG was screened and optimized using the central composite design, Franz diffusion cell experiments, and the split-split plot design. Skin irritation tests were performed in guinea pigs. The anti-gout effects were evaluated using mice. The optimized B-MEG comprised of 50 % pH 7.4 phosphate buffered saline, 4.48 % ethyl oleate, 31.64 % tween 80, 13.88 % glycerin, 2 % borneol, 0.5 % clove oil and 0.5 % xanthan gum, with a baicalin content of (10.42 ± 0.08) mg/g and particle size of (15.71 ± 0.41) nm. After 12 h, the cumulative amount of baicalin permeated from B-MEG was (672.14 ± 44.11) μg·cm−2. No significant skin irritation was observed following B-MEG application. Compared to the model group, B-MEG groups significantly decreased the rate of auricular swelling (P < 0.01) and number of twists observed in mice (P < 0.01); and also reduced the rate of paw swelling (P < 0.01) and inflammatory cell infiltration in a mouse model of GA. In conclusion, B-MEG represents a promising transdermal carrier for baicalin delivery and can be used as a potential therapy for GA.
作者机构：
[Cheng, Qingzhou; Cheng, Chu; Zhou, Wei; Chen, Yulong; Liu, Wenbin] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.;[Zhang, Zhiling; Ye, Jingsi] Huaren Technol Co Ltd, Wuhu, Peoples R China.;[Xiao, Pengfeng] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing, Peoples R China.
通讯机构：
[Cheng, C ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.
摘要：
Eliminating errors in next-generation sequencing has proven to be challenging. Here we present a novel strategy for DNA sequencing, called correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry. This sequencing method involves introducing a mixture of natural nucleotide X, labeled unblocked nucleotide X ', 3 ' blocked nucleotide Y*, and labeled 3 ' blocked nucleotide Y* into each reaction cycle. By cyclically interrogating a template twice with different nucleotide combinations, two sets of base-encoding are sequentially obtained, enabling accurate deduction of base sequence. We demonstrate the remarkable efficacy of this approach in detecting and correcting sequencing errors, achieving a theoretical error rate of 0.0005%, which is twice as accurate as Sanger sequencing. Furthermore, we show the capability to detect known mutation sites using information from only a single sequencing run. The correctable two-color fluorogenic DNA decoding sequencing approach should enable accurate identification of extremely rare genomic variations in diverse applications in biology and medicine. A correctable two-color fluorogenic DNA decoding sequencing, which can significantly improve sequencing accuracy and throughput by employing a dual-nucleotide addition combined with fluorogenic sequencing-by-synthesis (SBS) chemistry.
摘要：
Background Our previous multicenter case-control study showed that aging, up-regulation of platelet glycogen synthase kinase-3 beta (GSK-3 beta), impaired olfactory function, and ApoE & varepsilon;4 genotype were associated with cognitive decline in type 2 diabetes mellitus (T2DM) patients. However, the causal relationship between these biomarkers and the development of cognitive decline in T2DM patients remains unclear. Methods To further investigate this potential relationship, we designed a 6-year follow-up study in 273 T2DM patients with normal cognitive in our previous study. Baseline characteristics of the study population were compared between T2DM patients with and without incident mild cognitive impairment (MCI). We utilized Cox proportional hazard regression models to assess the risk of cognitive impairment associated with various baseline biomarkers. Receiver operating characteristic curves (ROC) were performed to evaluate the diagnostic accuracy of these biomarkers in predicting cognitive impairment. Results During a median follow-up time of 6 years (with a range of 4 to 9 years), 40 patients (16.13%) with T2DM developed MCI. Participants who developed incident MCI were more likely to be older, have a lower education level, have more diabetic complications, a higher percentage of ApoE & varepsilon;4 allele and a higher level of platelet GSK-3 beta activity (rGSK-3 beta) at baseline (P<0.05). In the longitudinal follow-up, individuals with higher levels of rGSK-3 beta were more likely to develop incident MCI, with an adjusted hazard ratio (HR) of 1.60 (95% confidence interval [CI] 1.05, 2.46), even after controlling for potential confounders. The AUC of the combination of age, rGSK-3 beta and ApoE & varepsilon;4 allele predicted for incident MCI was 0.71. Conclusion Platelet GSK-3 beta activity could be a useful biomarker to predict cognitive decline, suggesting the feasibility of identifying vulnerable population and implementing early prevention for dementia.
期刊：
Journal of Pharmaceutical and Biomedical Analysis,2024年249:116397 ISSN：0731-7085
通讯作者：
Cheng, C
作者机构：
[Cheng, Qingzhou; Cheng, Chu; Zhou, Wei; Chen, Yulong] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.;[Xiao, Pengfeng] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing, Peoples R China.
通讯机构：
[Cheng, C ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan, Peoples R China.
关键词：
Decoding sequencing;High accuracy;Long read length;Rare mutations;Single-color fluorogenic DNA
摘要：
We proposed a single-color fluorogenic DNA decoding sequencing method designed to improve sequencing accuracy, increase read length and throughput, as well as decrease scanning time. This method involves the incorporation of a mixture of four types of 3’-O-modified nucleotide reversible terminators into each reaction. Among them, two nucleotides are labeled with the same fluorophore, while the remaining two are unlabeled. Only one nucleotide can be extended in each reaction, and an encoding that partially defines base composition can be obtained. Through cyclic interrogation of a template twice with different nucleotide combinations, two sets of encodings are sequentially obtained, enabling the determination of the sequence. We demonstrate the feasibility of this method using established sequencing chemistry, achieving a cycle efficiency of approximately 99.5 %. Notably, this strategy exhibits remarkable efficacy in the detection and correction of sequencing errors, achieving a theoretical error rate of 0.00016 % at a sequencing depth of ×2, which is lower than Sanger sequencing. This method is theoretically compatible with the existing sequencing-by-synthesis (SBS) platforms, and the instrument is simpler, which may facilitate further reductions in sequencing costs, thereby broadening its applications in biology and medicine. Moreover, we demonstrate the capability to detect known mutation sites using information from only a single sequencing run. We validate this approach by accurately identifying a mutation site in the human mitochondrial DNA.
We proposed a single-color fluorogenic DNA decoding sequencing method designed to improve sequencing accuracy, increase read length and throughput, as well as decrease scanning time. This method involves the incorporation of a mixture of four types of 3’-O-modified nucleotide reversible terminators into each reaction. Among them, two nucleotides are labeled with the same fluorophore, while the remaining two are unlabeled. Only one nucleotide can be extended in each reaction, and an encoding that partially defines base composition can be obtained. Through cyclic interrogation of a template twice with different nucleotide combinations, two sets of encodings are sequentially obtained, enabling the determination of the sequence. We demonstrate the feasibility of this method using established sequencing chemistry, achieving a cycle efficiency of approximately 99.5 %. Notably, this strategy exhibits remarkable efficacy in the detection and correction of sequencing errors, achieving a theoretical error rate of 0.00016 % at a sequencing depth of ×2, which is lower than Sanger sequencing. This method is theoretically compatible with the existing sequencing-by-synthesis (SBS) platforms, and the instrument is simpler, which may facilitate further reductions in sequencing costs, thereby broadening its applications in biology and medicine. Moreover, we demonstrate the capability to detect known mutation sites using information from only a single sequencing run. We validate this approach by accurately identifying a mutation site in the human mitochondrial DNA.
通讯机构：
[Wei, N ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Dept Rehabil Sci, Wuhan, Hubei, Peoples R China.
关键词：
Dynapenia;anthropometric measurements;cut-off values;receiver operating characteristic curve analysis;the five times sit-to-stand test
摘要：
PURPOSE: This study evaluated whether anthropometric measurements and the five times sit-to-stand test could be used to identify dynapenia. The cut-off values of accurate screening tools for identifying dynapenia were also established. MATERIALS AND METHODS: This was a cross-sectional study conducted on individuals ≥ 60 years old (N = 529). All participants underwent handgrip strength measurement, anthropometric measurements and the five times sit-to-stand test. The participants whose handgrip strength was < 28 kg for men and < 18 kg for women were considered to have dynapenia. The association between the recorded variables and dynapenia was determined using logistic regression, and cut-off values were established by performing the Receiver Operating Characteristic curve analysis. RESULTS: The prevalence of dynapenia was 35.42% in men and 25.61% in women. For males, both calf circumference (≤ 35.2 cm) and the five times sit-to-stand test (≥ 14.6 s) could be used as accurate tools for dynapenia. For females, only the five times sit-to-stand test (≥ 11.8 s) had sufficient accuracy to be used as a screening tool for dynapenia. CONCLUSIONS: The five times sit-to-stand test was an accurate screening tool for identifying dynapenia. The calf circumference could be only used as a screening tool in males.
通讯机构：
[Cheng, QZ ; Ding, H ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.;Wuhan Univ, Sch Pharmaceut Sci, 185 Donghu Rd, Wuhan 430072, Hubei, Peoples R China.
关键词：
Epilepsy;Luteolin;Neuronal apoptosis;Neuroinflammation;TLR4/I kappa B alpha/NF-kappa B pathway
摘要：
Epilepsy represents a prevalent neurological disorder in the population, and the existing antiepileptic drugs (AEDs) often fail to adequately control seizures. Inflammation is recognized as a pivotal factor in the pathophysiology of epilepsy. Luteolin, a natural flavonoid extract, possesses anti-inflammatory properties and exhibits promising neuroprotective activity. Nevertheless, the precise molecular mechanisms underlying the antiepileptic effects of luteolin remain elusive. In this study, we established a rat model of epilepsy using pentylenetetrazole (PTZ) to induce seizures. A series of behavioral experiments were conducted to assess behavioral abilities and cognitive function. Histological techniques, including HE staining, Nissl staining, and TUNEL staining, were employed to assess hippocampal neuronal damage. Additionally, Western blotting, RT-qPCR, and ELISA were utilized to analyze the expression levels of proteins involved in the TLR4/IκBα/NF-κB signaling pathway, transcription levels of apoptotic factors, and levels of inflammatory cytokines, respectively. Luteolin exhibited a dose-dependent reduction in seizure severity, prolonged the latency period of seizures, and shortened seizure duration. Furthermore, luteolin prevented hippocampal neuronal damage in PTZ-induced epileptic rats and partially restored behavioral function and learning and memory abilities. Lastly, PTZ kindling activated the TLR4/IκBα/NF-κB pathway, leading to elevated levels of the cytokines TNF-α, IL-6 and IL-1β, which were attenuated by luteolin. Luteolin exerted anticonvulsant and neuroprotective activities in the PTZ-induced epileptic model. Its mechanism was associated with the inhibition of the TLR4/IκBα/NF-κB pathway, alleviating the immune-inflammatory response in the post-epileptic hippocampus.
Epilepsy represents a prevalent neurological disorder in the population, and the existing antiepileptic drugs (AEDs) often fail to adequately control seizures. Inflammation is recognized as a pivotal factor in the pathophysiology of epilepsy. Luteolin, a natural flavonoid extract, possesses anti-inflammatory properties and exhibits promising neuroprotective activity. Nevertheless, the precise molecular mechanisms underlying the antiepileptic effects of luteolin remain elusive. In this study, we established a rat model of epilepsy using pentylenetetrazole (PTZ) to induce seizures. A series of behavioral experiments were conducted to assess behavioral abilities and cognitive function. Histological techniques, including HE staining, Nissl staining, and TUNEL staining, were employed to assess hippocampal neuronal damage. Additionally, Western blotting, RT-qPCR, and ELISA were utilized to analyze the expression levels of proteins involved in the TLR4/IκBα/NF-κB signaling pathway, transcription levels of apoptotic factors, and levels of inflammatory cytokines, respectively. Luteolin exhibited a dose-dependent reduction in seizure severity, prolonged the latency period of seizures, and shortened seizure duration. Furthermore, luteolin prevented hippocampal neuronal damage in PTZ-induced epileptic rats and partially restored behavioral function and learning and memory abilities. Lastly, PTZ kindling activated the TLR4/IκBα/NF-κB pathway, leading to elevated levels of the cytokines TNF-α, IL-6 and IL-1β, which were attenuated by luteolin. Luteolin exerted anticonvulsant and neuroprotective activities in the PTZ-induced epileptic model. Its mechanism was associated with the inhibition of the TLR4/IκBα/NF-κB pathway, alleviating the immune-inflammatory response in the post-epileptic hippocampus.
摘要：
With the aging of human society, more and more elderly patients have to undergo surgery and anesthesia. Clinical observations have indicated from time to time that spinal anesthesia in the elderly appears to last longer than in young people, although there is limited research in this area and the mechanism is unclear at present time. This research work is expected to help understand the decline of local anesthetic metabolism in cerebrospinal fluid of elderly patients so as to help them with precise anesthesia and rapid rehabilitation. Twenty patients with spinal anesthesia in orthopedic lower limb surgery were selected to study the rate of drug metabolism in cerebrospinal fluid in two age groups, i.e.,18–30 years old and 75–90 years old. Ropivacaine in peripheral blood is used as a probe to reflect the speed of drug metabolism in cerebrospinal fluid. The contents of total Aβ protein and hyaluronic acid in the cerebrospinal fluid were investigated as well. The equivalent dose of ropivacaine anesthetizes the elderly group for a longer time. The metabolism rate of ropivacaine in an elderly patient was slower than that of a young patient. No significant difference in total Aβ protein between the two groups was observed while hyaluronic acid in the elderly group was significantly higher than that in the young group. This study shows that the dose of ropivacaine should be reduced when used for anesthesia in elderly patients. The cumulation of ropivacaine and HA appears to imitate the degeneration of central lymphatic circulation metabolism in elderly people.
