天然维生素E和合成维生素E在猪营养中的研究进展
作者：
刘玉兰;肖勘
期刊：
动物营养学报 ,2020年32(6):2449-2453 ISSN：1006-267X
作者机构：
武汉轻工大学动物科学与营养工程学院,动物营养与饲料科学湖北省重点实验室,武汉 430023;[肖勘; 刘玉兰] 武汉轻工大学
关键词：
天然维生素E;合成维生素E;猪营养
摘要：
维生素E是一类脂溶性维生素,是维持动物正常生理机能必不可少的酚类化合物。维生素E具有广泛的生物学作用,如抗氧化、抗应激、提高免疫、促进生长、改善肉质等,是畜禽配合饲料中不可缺少的添加成分之一。维生素E来源于天然植物或人工合成,二者在体内的吸收、转运存在差异,生物学效价也不一样。总体而言,天然维生素E在生物学利用率比合成维生素E高,其在提高仔猪免疫力、改善肥育猪肉质等方面比合成维生素E具有潜在优势。本文主要综述了天然维生素E和合成维生素E在猪营养中的应用,以期为养猪生产中维生素E的选择提供参考。
语种：
中文
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Effect of flaxseed oil on muscle protein loss and carbohydrate oxidation impairment in a pig model after lipopolysaccharide challenge.
作者：
Kang, Ping;Wang, Yang;Li, Xiangen;Wan, Zhicheng;Wang, Xiuying;...
期刊：
British Journal of Nutrition ,2020年123(8):859-869 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Wang, Yang; Zhao, Shengjun; Liu, Yulan; Chen, Huifu; Kang, Ping; Wang, Chunwei; Li, Xiangen; Wan, Zhicheng; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Carbohydrate oxidation impairment;Flaxseed oil;Muscle atrophy;Protein kinase B/Forkhead box O signalling pathways;Toll-like receptor 4/nucleotide-binding oligomerisation domain protein signalling pathway
摘要：
<jats:title>Abstract</jats:title><jats:p>Flaxseed oil is rich in α-linolenic acid (ALA), which is the metabolic precursor of EPA and DHA. The present study investigated the effect of flaxseed oil supplementation on lipopolysaccharide (LPS)-induced muscle atrophy and carbohydrate oxidation impairment in a piglet model. Twenty-four weaned pigs were used in a 2 × 2 factorial experiment including dietary treatment (5 % maize oil<jats:italic>v</jats:italic>. 5 % flaxseed oil) and LPS challenge (saline<jats:italic>v</jats:italic>. LPS). On day 21 of treatment, the pigs were injected intraperitoneally with 100 μg/kg body weight LPS or sterile saline. At 4 h after injection, blood, gastrocnemius muscle and longissimus dorsi muscle were collected. Flaxseed oil supplementation increased ALA, EPA, total<jats:italic>n</jats:italic>-3 PUFA contents, protein:DNA ratio and pyruvate dehydrogenase complex quantity in muscles (<jats:italic>P</jats:italic>< 0·05). In addition, flaxseed oil reduced mRNA expression of toll-like receptor (TLR) 4 and nucleotide-binding oligomerisation domain protein (NOD) 2 and their downstream signalling molecules in muscles and decreased plasma concentrations of TNF-<jats:italic>α</jats:italic>, IL-6 and IL-8, and mRNA expression of TNF-<jats:italic>α</jats:italic>, IL-1<jats:italic>β</jats:italic>and IL-6 (<jats:italic>P</jats:italic>< 0·05). Moreover, flaxseed oil inclusion increased the ratios of phosphorylated protein kinase B (Akt) 1:total Akt1 and phosphorylated Forkhead box O (FOXO) 1:total FOXO1 and reduced mRNA expression of FOXO1, muscle RING finger (MuRF) 1 and pyruvate dehydrogenase kinase 4 in muscles (<jats:italic>P</jats:italic>< 0·05). These results suggest that flaxseed oil might have a positive effect on alleviating muscle protein loss and carbohydrates oxidation impairment induced by LPS challenge through regulation of the TLR4/NOD and Akt/FOXO signalling pathways.</jats:p>
语种：
英文
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Prevalence of aflatoxin M1 in raw milk and three types of liquid milk products in central-south China
作者：
Xiong, Jianglin;Peng, Lijuan;Zhou, Hualin;Lin, Bo;Yan, Pingyu;...
期刊：
Food Control ,2020年108:106840 ISSN：0956-7135
通讯作者：
Wu, Lingying
作者机构：
[Liu, Yulan; Wu, Lingying; Xiong, Jianglin; Qiu, Yinsheng; Yan, Pingyu] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China.;[Lin, Bo] Guangxi Univ, Coll Anim Sci, Nanning 530004, Peoples R China.;[Wu, Wenxuan] Guizhou Univ, Coll Anim Sci, Guiyang 550025, Guizhou, Peoples R China.
通讯机构：
[Wu, Lingying] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Aflatoxin M1;China;Milk products;Raw milk
摘要：
The objective of this study was to survey the prevalence of aflatoxin M1 (AFM1) in raw, pasteurized, extended shelf life (ESL) and UHT milk from central-south China. A total of 734 milk samples, comprising raw milk (133 samples), pasteurized milk (410), ESL milk (93) and UHT milk (98), were obtained between Nov. 01, 2018 and Mar. 31, 2019. The AFM1 concentrations in milk samples were detected by ELISA and confirmed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Five hundred and ninety samples (80.4%) were positive for AFM1; the mean value was 23.7 ng/L, and the range was 5.1–104.4 ng/L. The percentages of AFM1 positive raw, pasteurized, ESL and UHT milk samples were 75.2, 82.2, 81.7 and 78.6%, respectively. The AFM1 concentration in raw milk was significantly lower (p < 0.05) than that in pasteurized and ESL milk, and the AFM1 level in UHT milk tended to be lower (p < 0.10) than that in pasteurized and ESL milk. The AFM1 levels exceeded the EU legal limit of 50 ng/L in 0, 11.5, 18.3 and 3.1% of raw, pasteurized, ESL and UHT milk samples, respectively. All milk samples tested had levels far below China's legal limit of 500 ng/L. Therefore, the AFM1 content in raw milk and milk products in central-south China does not pose a public health threat, but close attention should nonetheless be paid to milk AFM1, given the high positive rate of 80.4%. © 2019
语种：
英文
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EPA and DHA Inhibit Myogenesis and Downregulate the Expression of Muscle-related Genes in C2C12 Myoblasts
作者：
Zhang, Jing;Xu, Xin;Liu, Yan;Zhang, Lin;Odle, Jack;...
期刊：
Genes ,2019年10(1):64 ISSN：2073-4425
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yan; Zhang, Jing; Liu, Yulan; Xu, Xin; Zhang, Lin; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
C2C12;proliferation;differentiation;EPA;DHA
摘要：
This study was conducted to elucidate the biological effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cell proliferation, differentiation and gene expression in C2C12 myoblasts. C2C12 were treated with various concentrations of EPA or DHA under proliferation and differentiation conditions. Cell viability was analyzed using cell counting kit-8 assays (CCK-8). The Edu assays were performed to analyze cell proliferation. To analyze cell differentiation, the expressions of myogenic marker genes were determined at the transcriptional and translational levels by qRT-PCR, immunoblotting and immunofluorescence. Global gene expression patterns were characterized using RNA-sequencing. Phosphorylation levels of ERK and Akt were examined by immunoblotting. Cell viability and proliferation was significantly inhibited after incubation with EPA (50 and 100 muM) or DHA (100 muM). Both EPA and DHA suppressed C2C12 myoblasts differentiation. RNA-sequencing analysis revealed that some muscle-related genes were significantly downregulated following EPA or DHA (50 muM) treatment, including insulin-like growth factor 2 (IGF-2), troponin T3 (Tnnt3), myoglobin (Mb), myosin light chain phosphorylatable fast skeletal muscle (Mylpf) and myosin heavy polypeptide 3 (Myh3). IGF-2 was crucial for the growth and differentiation of skeletal muscle and could activate the PI3K/Akt and the MAPK/ERK cascade. We found that EPA and DHA (50 muM) decreased the phosphorylation levels of ERK1/2 and Akt in C2C12 myoblasts. Thus, this study suggested that EPA and DHA exerted an inhibitory effect on myoblast proliferation and differentiation and downregulated muscle-related genes expression.
语种：
英文
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脂多糖刺激对断奶仔猪脾脏炎症信号通路关键基因表达的影响
作者：
汪洋;李先根;刘玉兰;朱惠玲;王秀英
期刊：
动物营养学报 ,2019年31(8):3882-3890 ISSN：1006-267X
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉,430023;[汪洋; 李先根; 王秀英; 朱惠玲; 刘玉兰] 武汉轻工大学
关键词：
脂多糖;断奶仔猪;脾脏;炎症
摘要：
本试验旨在研究脂多糖(LPS)刺激后不同时间断奶仔猪脾脏Toll样受体4( TLR4)和核苷酸结合寡聚域受体( NOD)炎症信号通路关键基因表达的变化.选择42 头体重为( 7.1 ± 0.9) kg的杜×长×大断奶仔猪,按注射LPS之前( 0 h)和注射LPS后不同时间点( 1、2、4、8、12、24 h)随机分为7个处理,每个处理6头猪.预饲14 d后,腹腔注射100 μg／kg BW的LPS,分别于注射LPS之前和注射LPS后不同时间点,将仔猪麻醉屠宰,取血液和脾脏样品,测定血细胞分类计数和脾脏炎症信号通路关键基因及炎症相关基因的mRNA表达量.结果表明:LPS刺激导致部分血细胞分类计数发生显著的变化( P＜0.05) ;使 TLR4 和 NOD 炎症信号通路关键基因mRNA表达量显著上调( P＜0.05) ,且在2～8 h达到峰值;也导致肿瘤坏死因子－α、白细胞介素－1β、白细胞介素－6、环氧合酶2、热休克蛋白70 mRNA表达量显著升高( P＜0.05),且在1～4 h达到峰值. LPS刺激8～24 h后,这些炎症相关基因均逐渐恢复至正常水平或略低于正常水平.总体来看,LPS刺激激活了炎症信号通路,促进炎性细胞因子的表达,并随着时间的延长,这些基因呈现先上升、达到高峰后逐渐下降的变化.
语种：
中文
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Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide.
作者：
Wang, Xiuying;Wang, Wenjun;Wang, Longmei;Yu, Cheng;Zhang, Guolong;...
期刊：
FOOD & FUNCTION ,2019年10(1):479-489 ISSN：2042-6496
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Guolong; Zhao, Shengjun; Hu, Chien-An Andy; Liu, Yulan; Wang, Chunwei; Yu, Cheng; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Yu, Cheng; Wang, Wenjun] South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Hubei, Peoples R China.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
摘要：
The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change. Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases. We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model. Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline). After feeding basal or LNT diet for 21 days, pigs were injected with LPS or saline. At 4 h post-injection, pigs were killed and jejunum, ileum and cecal digesta were collected. LNT improved intestinal morphology and barrier function. LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine. In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor. Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels. Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota. These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones. © 2019 The Royal Society of Chemistry.
语种：
英文
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Long non-coding RNA profiling in LPS-induced intestinal inflammation model: New insight into pathogenesis:
作者：
Guo, Ling;Li, Linna;Zhang, Yang;Fu, Shulin;Zhang, Jing;...
期刊：
INNATE IMMUNITY ,2019年25(8):491-502 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Yang; Zhang, Jing; Wu, Lingying; Liu, Yulan; Li, Linna; Fu, Shulin; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Yang; Zhang, Jing; Wu, Lingying; Liu, Yulan; Fu, Shulin; Wang, Xiuying; Guo, Ling; Zhu, Huiling] Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan, Hubei, Peoples R China.;[Qiao, Mu] Hubei Acad Agr Sci, Key Lab Anim Embryo Engn & Mol Breeding Hubei Pro, Inst Anim Husb & Vet, Wuhan, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Long non-coding RNA;lipopolysaccharide;intestinal inflammation;cam signalling pathway;mTOR signalling pathway
摘要：
LPS can induce an inflammatory immune response in the intestine, and long non-coding RNA (lncRNA) is involved in the process of inflammatory disease. However, the biological role of lncRNA in the intestinal inflammation of piglets remains unclear. In this study, the lncRNA expression profile of the ileal mucosa of piglets challenged by LPS was analysed using lncRNA sequencing. In total, 112 novel lncRNAs were predicted, of which 58 were up-regulated and 54 down-regulated following LPS challenge. Expression of 15 selected lncRNAs was validated by quantitative PCR. We further investigated the target genes of lncRNA that were enriched in the signalling pathways involved in the inflammatory immune response by utilising Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes analysis, with cell adhesion molecules and mTOR signalling pathway identified. In addition, the co-expression networks between the differentially expressed lncRNAs and the target mRNAs were constructed, with seven core lncRNAs identified, which also demonstrated that the relationship between lncRNAs and the target genes was highly correlated. Our study offers important information about the lncRNAs of the mucosal immune system in piglets and provides new insights into the inflammatory mechanism of LPS challenge, which might serve as a novel target to control intestinal inflammation.
语种：
英文
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Dietary fish oil supplementation alters liver gene expressions to protect against LPS-induced liver injury in weanling piglets
作者：
Zhang, Jing;Xu, Xin;Zhu, Huiling;Wang, Yang;Hou, Yongqing;...
期刊：
INNATE IMMUNITY ,2019年25(1):60-72 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Wang, Yang; Zhang, Jing; Liu, Yulan; Hou, Yongqing; Xu, Xin; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Fish oil;inflammation;lipopolysaccharide;liver;RNA sequencing;pig
摘要：
Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury. © The Author(s) 2018.
语种：
英文
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脂多糖刺激对断奶仔猪肌肉炎症和蛋白质降解相关基因表达的影响
作者：
李先根;汪洋;王秀英;朱惠玲;刘玉兰
期刊：
中国畜牧杂志 ,2019年55(4):74-77 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[汪洋; 李先根; 王秀英; 刘玉兰; 朱惠玲] 武汉轻工大学
关键词：
断奶仔猪;脂多糖;炎症;肌肉蛋白质降解
摘要：
本试验旨在研究脂多糖(LPS)刺激后不同时间断奶仔猪肌肉炎症和肌肉蛋白质降解相关基因表达的变化规律.选择42头(7.1±0.9) kg杜×长×大三元杂断奶仔猪,按注射LPS之前(0 h)和注射LPS后1、2、4、8、12、24 h随机分为7个处理,每个处理6头猪.预试14d后,腹腔注射100 tg/kg体重的LPS.按以上时间点将仔猪屠宰,取背最长肌样品待测.结果 表明:背最长肌炎性细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6的mRNA表达量在注射LPS 1～2 h后达到峰值;Toll样受体4信号通路关键基因Toll样受体4(TLR4)、骨髓分化因子88 (MyD88),核苷酸结合寡聚域信号通路关键基因核苷酸结合寡聚域2 (NOD2)、受体互作蛋白激酶2(RIPK2)的mRNA表达量在注射LPS 2～4 h后达到峰值;肌肉蛋白质降解相关基因叉头转录因子-1 (FOXO-1)、FOXO-4、肌肉环指蛋白1(MuRF1)、肌萎缩F-box (MAFbx)的mRNA表达量在注射LPS 12 h后达到峰值.可见,LPS刺激诱导肌肉释放大量炎性细胞因子,使TLR4和NOD炎症信号通路关键基因及肌肉蛋白质降解相关基因mRNA显著表达.
语种：
中文
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Fermented cassava bioethanol waste as substitute of protein in diet for growth performance and carcass evaluation on meat ducks.
作者：
Lei, Long;Feng, Zhi;Li, Qiang;Xue, Xiaoqiang;Zhang, Dandan;...
期刊：
Tropical Animal Health and Production ,2019年51(5):1049-1056 ISSN：0049-4747
通讯作者：
Zhao, Shengjun
作者机构：
[Li, Qiang; Zhao, Shengjun; Lei, Long; Liu, Zhengya; Liu, Yulan; Xue, Xiaoqiang; Feng, Zhi; Zhang, Dandan; Ren, Ying] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhao, Shengjun] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
Digestibility rate;Fermented cassava bioethanol waste;Growth performance;Meat duck;Meat quality
摘要：
The effect of dietary supplementation with fermented cassava bioethanol waste (FCBW) on the growth performance and meat quality was evaluated in 80 15-day-old male Cherry Valley meat ducks with an initial body weight (BW) of 250.67 +/- 7.50 g. The experiment has 5 replications and 4 treatments and 4 ducks per treatment. Four groups (groups I, II, III, IV) supplemented with 0%, 5%, 10%, and 15% FCBW substituted for part of maize, soybean meal, and bran in basal diet and were fed for 29 days; the metabolizable energy and content of lysine in the four groups were equal. The results indicated that there were no significant differences in average daily weight gain and average daily feed intake among the four groups (P > 0.05). The digestibility rate of dry matter, ash, and phosphorus in group IV was significantly lower than that in group I by 5.23%, 6.25%, and 6.40% respectively (P < 0.05), but the digestibility rate of crude fat was significantly higher than that in group I by 8.30% (P < 0.05). No significant differences were presented among different levels of FCBW supplementation in carcass yield, eviscerated carcass yield, and semi-eviscerated carcass yield (P > 0.05), but 5% FCBW can improve the carcass yield relatively. In conclusion, with dietary supplementation of 5% FCBW, a better growth performance in meat ducks could be achieved.
语种：
英文
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Flaxseed Oil Attenuates Intestinal Damage and Inflammation by Regulating Necroptosis and TLR4/NOD Signaling Pathways Following Lipopolysaccharide Challenge in a Piglet Model
作者：
Zhu, Huiling;Wang, Haibo;Wang, Shuhui;Tu, Zhixiao;Zhang, Lin;...
期刊：
MOLECULAR NUTRITION & FOOD RESEARCH ,2018年62(9):e1700814 ISSN：1613-4125
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Hou, Yongqing; Wang, Haibo; Wang, Shuhui; Wang, Chunwei; Tu, Zhixiao; Zhang, Lin; Wang, Xiuying; Chen, Jie; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词：
flaxseed oil;inflammation;intestinal barrier function;lipopolysaccharide;necroptosis signaling pathway;weaned pigs
摘要：
Scope: Flaxseed oil is a rich source of α-linolenic acid (ALA), which is the precursor of the long-chain n-3 polyunsaturated fatty acids (PUFAs), including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). This study investigates the protective effect of flaxseed oil against intestinal injury induced by lipopolysaccharide (LPS). Materials and results: Twenty-four weaned pigs were used in a 2 × 2 factorial experiment with dietary treatment (5% corn oil vs 5% flaxseed oil) and LPS challenge (saline vs LPS). On day 21 of the experiment, pigs were administrated with LPS or saline. At 2 h and 4 h post-administration, blood samples were collected. After the blood harvest at 4 h, all piglets were slaughtered and intestinal samples were collected. Flaxseed oil supplementation led to the enrichment of ALA, EPA, and total n-3 PUFAs in intestine. Flaxseed oil improved intestinal morphology, jejunal lactase activity, and claudin-1 protein expression. Flaxseed oil downregulated the mRNA expression of intestinal necroptotic signals. Flaxseed oil also downregulated the mRNA expression of intestinal toll-like receptors 4 (TLR4) and its downstream signals myeloid differentiation factor 88 (MyD88), nuclear factor kappa B (NF−κB), and nucleotide-binding oligomerization domain proteins 1, 2 (NOD1, NOD2) and its adapter molecule, receptor-interacting protein kinase 2 (RIPK2). Conclusion: These results suggest that dietary addition of flaxseed oil enhances intestinal integrity and barrier function, which is involved in modulating necroptosis and TLR4/NOD signaling pathways. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
语种：
英文
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Glycine relieves intestinal injury by maintaining mtor signaling and suppressing AMPK, TLR4, and NOD signaling in weaned piglets after lipopolysaccharide challenge
作者：
Xu, Xiao;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;Liu, Congcong;...
期刊：
International Journal of Molecular Sciences ,2018年19(7):1980 ISSN：1661-6596
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Liu, Congcong; Xu, Xiao; Liu, Yulan; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Dai, Bing; Liu, Xiuting] Zhe Jiang Goshine Test Technol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
adenosine triphosphate;glycine;iron;lipopolysaccharide;mammalian target of rapamycin;messenger RNA;rapamycin;sodium chloride;toll like receptor 4;zinc;AMP-activated protein kinase kinase;glycine;lipopolysaccharide;protein kinase;target of rapamycin kinase;toll like receptor 4;animal experiment;animal model;Article;colitis;controlled study;diet supplementation;Escherichia coli;gene expression;immunoblotting;inflammation;intestine injury;nonhuman;protein degradation;protein expression;real time polymerase chain reaction;RNA extraction;signal transduction;Western blotting;animal;drug effect;genetics;ileum;immunology;intestine mucosa;intraperitoneal drug administration;jejunum;metabolism;pathology;pig;weaning;Animals;Escherichia coli;Glycine;Ileum;Inflammation;Injections, Intraperitoneal;Intestinal Mucosa;Jejunum;Lipopolysaccharides;Models, Animal;Protein Kinases;RNA, Messenger;Swine;Toll-Like Receptor 4;TOR Serine-Threonine Kinases;Weaning
摘要：
This study was conducted to envaluate whether glycine could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by regulating intestinal epithelial energy status, protein synthesis, and inflammatory response via AMPK, mTOR, TLR4, and NOD signaling pathways. A total of 24 weanling piglets were randomly allotted to 1 of 4 treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 1% glycine; (4) LPS + 2% glycine. After 28 days feeding, piglets were injected intraperitoneally with saline or LPS. The pigs were slaughtered and intestinal samples were collected at 4 h postinjection. The mRNA expression of key genes in these signaling pathways was measured by real-time PCR. The protein abundance was measured by Western blot analysis. Supplementation with glycine increased jejunal villus height/crypt depth ratio. Glycine also increased the jejunal and ileal protein content, RNA/DNA ratio, and jejunal protein/DNA ratio. The activities of citroyl synthetase in ileum, and α-ketoglutarate dehydrogenase complex in jejunum, were increased in the piglets fed diets supplemented with glycine. In addition, glycine decreased the jejunal and ileal phosphorylation of AMPKα, and increased ileal phosphorylation of mTOR. Furthermore, glycine downregulated the mRNA expression of key genes in inflammatory signaling. Meanwhile, glycine increased the mRNA expression of negative regulators of inflammatory signaling. These results indicate that glycine supplementation could improve energy status and protein synthesis by regulating AMPK and mTOR signaling pathways, and relieve inflammation by inhibiting of TLR4 and NOD signaling pathways to alleviate intestinal injury in LPS-challenged piglets. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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亚麻籽油对脂多糖刺激断奶仔猪肠黏膜结构和免疫细胞的影响
作者：
李先根;涂治骁;王树辉;王海波;张琳;...
期刊：
动物营养学报 ,2018年30(2):515-523 ISSN：1006-267X
作者机构：
[李先根; 涂治骁; 王树辉; 王海波; 张琳; 秦琴; 汪龙梅; 王秀英; 刘玉兰; 朱惠玲] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023
关键词：
亚麻籽油;断奶仔猪;脂多糖;肠黏膜免疫细胞
摘要：
本试验旨在研究亚麻籽油对脂多糖(LPS)刺激断奶仔猪肠黏膜结构和免疫细胞的影响。选择24头(9.15±0.90) kg杜×长×大断奶仔猪,随机分为4组,每组6个重复,每个重复1头猪。试验采用2×2双因子设计,主因子包括:1)饲粮处理(5%玉米油或5%亚麻籽油) ;2)免疫应激(注射生理盐水或LPS) 。饲粮中添加5%玉米油或5%亚麻籽油饲喂21 d,在试验第21天,每组1 / 2的猪腹膜注射100 μg / kg BW的LPS,另外1 / 2的猪腹膜注射等量生理盐水。注射LPS或生理盐水4 h后,将仔猪麻醉屠宰,取肠道样品待测。结果表明:1) LPS刺激显著降低了空肠、回肠绒毛高度和空肠隐窝深度(P<0.05) ,显著提高空肠固有层细胞密度和嗜中性粒细胞数目(P<0.05) ,有降低空肠上皮间淋巴细胞数目(P = 0.080)和回肠杯状细胞数目(P = 0.059)的趋势;2) LPS刺激对亚麻籽油组仔猪空肠、回肠绒毛高度无显著影响(P>0.05) ;饲粮添加5%亚麻籽油有提高回肠派氏结细胞密度的趋势(P = 0.064) ,且在LPS刺激下,显著降低空肠固有层细胞密度(P<0.05) ,并有增加空肠上皮间淋巴细胞数目的趋势(P = 0.069) 。结果提示,5%亚麻籽油在一定程度上可维护LPS刺激断奶仔猪的小肠黏膜结构和免疫功能。
语种：
中文
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猪胚胎期小肠组织MKRN3基因启动子区CpG岛的甲基化模式分析
作者：
张阳;汪龙梅;郭玲;付书林;刘玉兰;...
期刊：
中国畜牧杂志 ,2018年54(6):38-42 ISSN：0258-7033
作者机构：
动物营养与饲料安全湖北省协同创新中心,动物营养与饲料科学湖北省重点实验室,武汉轻工大学动物科学与营养工程学院,湖北武汉430023;[汪龙梅; 陈洪波; 张阳; 郭玲; 张晶; 刘玉兰; 付书林] 武汉轻工大学
关键词：
DNA甲基化;重亚硫酸盐测序法;MKRN3基因;CpG岛
摘要：
为揭示猪MKRN3基因启动子区CpG岛在胚胎小肠组织的甲基化模式,本实验以梅山猪-大白猪正反交为模型,以65、100日龄的胚胎小肠组织为研究材料,首先利用生物信息学手段预测猪MKRN3基因的启动子区CpG岛,围绕预测的CpG岛设计引物,采用重亚硫酸盐测序法检测梅山猪、大白猪正反交不同发育时期胚胎小肠组织中目的基因的甲基化水平.结果表明:在大白×梅山65日龄胚胎小肠中,猪MKRN3基因启动子区CpG岛呈现高度甲基化(70.8％),而在梅山×大白65日龄胚胎小肠中呈现低甲基化(8.4％),正反交后代的甲基化模式截然相反;在大白×梅山100日龄胚胎小肠组织中,目的基因CpG岛呈现低甲基化(16.4％),而在梅山×大白100日龄胚胎小肠组织中的平均甲基化水平为37.2％.MKRN3基因启动子区CpG岛的甲基化模式随着正反交、胚胎发育时期不同而呈现出一定变化规律,推测该基因的DNA甲基化具有父母本等位基因偏好性,且在胚胎发育65～100日龄父本或母本等位基因可能会发生明显的去甲基化.本研究结果为进一步阐明猪MKRN3甲基化水平调控其基因表达及印记状态提供一定理论基础.
语种：
中文
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Dietary modulation of endogenous host defense peptide synthesis as an alternative approach to in-feed antibiotics
作者：
Robinson, Kelsy;Ma, Xi;Liu, Yulan;Qiao, Shiyan;Hou, Yongqing;...
期刊：
动物营养(英文) ,2018年4(2):160-169 ISSN：2405-6545
通讯作者：
Zhang, Guolong
作者机构：
[Zhang, Guolong; Robinson, Kelsy] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;[Qiao, Shiyan; Ma, Xi] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Zhang, Guolong; Liu, Yulan; Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Zhang, Guolong] O;[Zhang, Guolong] W;Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China.
关键词：
Host defense peptides;Antibiotic alternatives;Butyrate;Vitamin D;Antibiotics
摘要：
Traditionally, antibiotics are included in animal feed at subtherapeutic levels for growth promotion and disease prevention. However, recent links between in-feed antibiotics and a rise in antibiotic-resistant pathogens have led to a ban of all antibiotics in livestock production by the European Union in January 2006 and a removal of medically important antibiotics in animal feeds in the United States in January 2017. An urgent need arises for antibiotic alternatives capable of maintaining animal health and productivity without triggering antimicrobial resistance. Host defense peptides (HDP) are a critical component of the animal innate immune system with direct antimicrobial and immunomodulatory activities. While in-feed supplementation of recombinant or synthetic HDP appears to be effective in maintaining animal performance and alleviating clinical symptoms in the context of disease, dietary modulation of the synthesis of endogenous host defense peptides has emerged as a cost-effective, antibiotic-alternative approach to disease control and prevention. Several different classes of small-molecule compounds have been found capable of promoting HDP synthesis. Among the most efficacious compounds are butyrate and vitamin D. Moreover, butyrate and vitamin D synergize with each other in enhancing HDP synthesis. This review will focus on the regulation of HDP synthesis by butyrate and vitamin D in humans, chickens, pigs, and cattle and argue for potential application of HDP-inducing compounds in antibiotic-free livestock production.
语种：
英文
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Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide
作者：
Qin, Qin;Xu, Xiao;Wang, Xiuying;Wu, Huanting;Zhu, Huiling;...
期刊：
Scientific Reports ,2018年8(1):15124 ISSN：2045-2322
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Qin, Qin; Xu, Xiao; Liu, Yulan; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Dai, Bing; Liu, Xiuting] Zhe Jiang Goshine Test Technol Co Ltd, Hangzhou 310030, Zhejiang, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Nodal Signaling Pathway;IL-1 Receptor-associated Kinase (IRAK1);Myeloid Differentiation Factor (MyD88);Tollip;TNF Receptor Associated Factor 6 (TRAF6)
摘要：
<jats:title>Abstract</jats:title><jats:p>This experiment aimed to explore whether glutamate (Glu) had beneficial effects on intestinal injury caused by <jats:italic>Escherichia coli</jats:italic> LPS challenge via regulating mTOR, TLRs, as well as NODs signaling pathways. Twenty-four piglets were allotted to 4 treatments including: (1) control group; (2) LPS group; (3) LPS + 1.0% Glu group; (4) LPS + 2.0% Glu group. Supplementation with Glu increased jejunal villus height/crypt depth ratio, ileal activities of lactase, maltase and sucrase, and RNA/DNA ratio and protein abundance of claudin-1 in jejunum and ileum. In addition, the piglets fed Glu diets had higher phosphorylated mTOR (Ser<jats:sup>2448</jats:sup>)/total mTOR ratio in jejunum and ileum. Moreover, Glu decreased TNF-α concentration in plasma. Supplementation with Glu also decreased mRNA abundance of jejunal TLR4, MyD88, IRAK1, TRAF6, NOD2 and increased mRNA abundance of ileal Tollip. These results indicate that Glu supplementation may be closely related to maintaining mTOR and inhibiting TLR4 and NOD signaling pathways, and concomitant improvement of intestinal integrity under an inflammatory condition.</jats:p>
语种：
英文
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甘氨酸对脂多糖刺激引起的断奶仔猪肠道能量代谢紊乱的影响
作者：
余程;王秀英;吴欢听;张琳;刘玉兰;...
期刊：
中国饲料 ,2018年(9):47-51 ISSN：1004-3314
作者机构：
中南民族大学生命科学学院,湖北武汉,430074;武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[吴欢听; 王秀英; 张琳; 刘玉兰] 武汉轻工大学;[汪文俊; 余程] 中南民族大学
关键词：
仔猪;脂多糖;甘氨酸;肠道;能量代谢
摘要：
为研究甘氨酸(Gly)对脂多糖(LPS)刺激引起的断奶仔猪肠道能量代谢紊乱的影响,试验选用24头体重为(7.17±0.41)kg的健康断奶仔猪[(21±1)d],分为4个处理组,分别为对照组、LPS组、1.0% Gly组和2.0% Gly组.试验第28天,LPS组、1.0% Gly组和2.0% Gly组猪注射100 μg/kg BW 的LPS,对照组注射相同剂量的生理盐水,4 h后屠宰,采集小肠样品待测.结果表明:Gly提高了肠道腺苷酸含量和三羧酸循环关键酶活性,影响了能量代谢相关基因过氧化物酶体增殖物激活受体γ辅激活因子1α和沉默信号调控因子1 mRNA表达.以上结果表明,Gly可以缓解LPS刺激导致的肠道能量代谢紊乱.
语种：
中文
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Necrostatin-1对脂多糖刺激的断奶仔猪血细胞分类计数和血液生化指标的影响
作者：
汪洋;王秀英;汪龙梅;代兵;刘秀婷;...
期刊：
中国畜牧杂志 ,2018年54(8):80-86 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室;[刘秀婷; 代兵] 浙江国正检测技术有限公司;[汪龙梅; 汪洋; 王秀英; 刘玉兰] 武汉轻工大学
关键词：
血细胞分类计数;血液生化指标;仔猪
摘要：
本试验旨在研究程序性坏死阻断剂Necrostatin-1 (Nec-1)对脂多糖(LPS)刺激断奶仔猪血细胞分类计数和血液生化指标的影响.选用25头断奶仔猪,分为对照组[10％二甲基亚砜(DMSO)+生理盐水]、LPS组(10％DMSO+LPS)、0.33Nec-1组[0.33 mg/kg体重(BW) Nec-1+LPS]、1.0Nec-1组(1.0 mg/kg BW Nec-1+LPS)和3.3Nec-1组(3.3 mg/kg BW Nec-1+LPS).注射10％ DMSO或Nec-1 30 min后,对照组注射生理盐水,其他组注射100 μg/kg BW LPS,分别在注射LPS或生理盐水4h和24h后采血液样品待测.结果表明:注射LPS 4h后,0.33 mg/kg BW Nec-1、1.0 mg/kg BW Nec-1和3.3 mg/kg BW Nec-1显著缓解LPS刺激导致的血小板分布宽度升高(P＜0.05);0.33 mg/kg BW Nec-1能显著缓解LPS刺激导致的血浆谷草转氨酶(AST)活性和AST/谷丙转氨酶(ALT)比例的升高(P＜0.05),1.0 mg/kg BW Nec-1显著缓解血浆葡萄糖含量和肌酸激酶活性的降低(P＜0.05);注射LPS 24 h后,0.33 mg/kg BW Nec-1、1.0 mg/kg BW Nec-1和3.3 mg/kg BW Nec-1可显著缓解LPS刺激导致的嗜酸性粒细胞比例的升高(P＜0.05),并有缓解嗜酸性粒细胞绝对值升高的趋势(P＜0.10);0.33 mg/kg BWNec-1缓解了肌酸激酶活性的降低并提高了血浆总胆固醇和甘油三酯含量(P＜0.05),1.0 mg/kg BW Nec-1有缓解LPS刺激导致的血浆AST/ALT升高的趋势(P＜0.10),3.3 mg/kg BW Nec-1能显著缓解AST/ALT比例的升高(P＜0.05)和葡萄糖的降低(P＜0.05),并有缓解AST升高和总胆固醇降低的趋势(P＜0.10).以上结果表明,Nec-1对LPS刺激导致的免疫应激反应具有一定的缓解作用.
语种：
中文
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Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
作者：
Zhang, Lin;Wang, Xiuying;Chen, Shaokui;Wang, Shuhui;Tu, Zhixiao;...
期刊：
International Journal of Molecular Sciences ,2018年19(11):3697- ISSN：1661-6596
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Guolong; Liu, Yulan; Chen, Shaokui; Xiong, Jianglin; Wang, Shuhui; Tu, Zhixiao; Li, Xiangen; Zhang, Lin; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
alanine aminotransferase;alkaline phosphatase;aspartate aminotransferase;caspase recruitment domain protein 4;claudin 1;docosahexaenoic acid;heat shock protein 70;icosapentaenoic acid;interleukin 1beta;interleukin 6;lipopolysaccharide;medium chain triacylglycerol;messenger RNA;mitogen activated protein kinase 1;mitogen activated protein kinase 3;mitogen activated protein kinase p38;mixed lineage kinase domain like protein;phosphoglycerate mutase;polyunsaturated fatty acid;protein serine threonine kinase;toll like receptor 4;tumor necrosis factor;unclassified drug;alanine aminotransferase;alkaline phosphatase;antiinflammatory agent;caspase recruitment domain protein 4;cytokine;lipopolysaccharide;omega 3 fatty acid;toll like receptor 4;triacylglycerol;animal experiment;animal model;animal tissue;Article;autophagosome;cell infiltration;controlled study;diet supplementation;down regulation;enzyme linked immunosorbent assay;factorial design;histology;lipid composition;liver injury;membrane rupture;mitochondrion swelling;necroptosis;nonhuman;pig;protein expression;protein phosphorylation;real time polymerase chain reaction;renal protection;signal transduction;transmission electron microscopy;Western blotting;animal;apoptosis;blood;dietary supplement;drug effect;genetics;liver cell;liver disease;MAPK signaling;metabolism;randomization;Alanine Transaminase;Alkaline Phosphatase;Animals;Anti-Inflammatory Agents;Apoptosis;Cytokines;Dietary Supplements;Fatty Acids, Omega-3;Hepatocytes;Lipopolysaccharides;Liver Diseases;MAP Kinase Signaling System;Nod1 Signaling Adaptor Protein;Random Allocation;Swine;Toll-Like Receptor 4;Triglycerides
摘要：
This study was conducted to investigate whether medium-chain triglycerides (MCTs) attenuated lipopolysaccharide (LPS)-induced liver injury by down-regulating necroptotic and inflammatory signaling pathways. A total of 24 pigs were randomly allotted to four treatments in a 2 × 2 factorial design including diet (0 and 4% MCTs) and immunological challenge (saline and LPS). After three weeks of feeding with or without 4% MCTs, pigs were challenged with saline or LPS. MCTs led to a significant increase in eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acid concentrations. MCTs attenuated LPS-induced liver injury as indicated by an improvement in liver histomorphology and ultrastructural morphology of hepatocytes, a reduction in serum alanine aminotransferase and alkaline phosphatase activities as well as an increase in claudin-1 protein expression. In addition, MCTs also reduced serum tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 concentrations, liver TNF-α and IL-1β mRNA expression and protein concentrations and enhanced liver heat shock protein 70 protein expression in LPS-challenged pigs. Moreover, MCTs decreased mRNA expression of receptor-interacting serine/threonine-protein kinase (RIP) 3, mixed-lineage kinase domain-like protein (MLKL) and phosphoglycerate mutase 5 and inhibited MLKL phosphorylation in the liver. Finally, MCTs decreased liver mRNA expression of toll-like receptor (TLR) 4, nucleotide-binding oligomerization domain protein (NOD) 1 and multiple downstream signaling molecules. MCTs also suppressed LPS-induced p38 mitogen-activated protein kinase (MAPK) phosphorylation and increased extracellular signal-related kinase 1/2 phosphorylation in the liver. These results indicated that MCTs are capable of attenuating LPS-induced liver damage by suppressing hepatic necroptotic (RIP1/RIP3/MLKL) and inflammatory (TLR4/NOD1/p38 MAPK) signaling pathways. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Flaxseed oil improves liver injury and inhibits necroptotic and inflammatory signaling pathways following lipopolysaccharide challenge in a piglet model
作者：
Wang, Longmei;Tu, Zhixiao;Wang, Haibo;Wang, Shuhui;Wang, Xiuying;...
期刊：
Journal of Functional Foods ,2018年46:482-489 ISSN：1756-4646
通讯作者：
Liu, Yulan
作者机构：
[Hu, Chien-An Andy; Liu, Yulan; Wang, Haibo; Wang, Shuhui; Tu, Zhixiao; Wang, Longmei; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Flaxseed oil;Inflammation;Lipopolysaccharide;Liver;Necroptosis;Pigs
摘要：
The hepatoprotective effects of flaxseed oil (FO) were tested in a pig model of lipopolysaccharide (LPS)-induced liver injury. Twenty-four piglets were used in a 2 × 2 factorial design including diet and an LPS challenge. After 3 weeks of feeding with 5% FO or 5% corn oil, pigs were challenged with LPS or saline. FO alleviated LPS-induced morphological liver damage, reduced serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities, and increased claudin-1 protein expression. FO decreased tumor necrosis factor-α interleukin-6 and cyclooxygenase 2 mRNA expression, and heat shock protein 70 mRNA and protein expression. FO downregulated receptor interacting protein kinase (RIP)1, RIP3, and mixed-lineage kinase domain-like protein (MLKL) mRNA expression, RIP1 and RIP3 protein expression, and MLKL phosphorylation. FO downregulated mRNA expression of toll-like receptor 4, nucleotide-binding oligomerization domain protein 1 and multiple downstream signaling molecules, and decreased p38 and extracellular signal-regulated kinase 1/2 phosphorylation. These results indicate that FO inhibits necroptotic and inflammatory signaling pathways to protect the liver from inflammation-related injury. © 2018 Elsevier Ltd
语种：
英文
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