酿酒酵母中柚皮素异源合成途径构建与优化
作者:
任枫;周一征;杨利文;王舜;何如怡;...
期刊:
武汉轻工大学学报 ,2024年43(04):49-57 ISSN:2095-7386
作者机构:
武汉轻工大学生命科学与技术学院,武汉 430023;[杨利文; 何如怡; 田宇; 周一征; 刘志国; 王舜; 任枫; 杨彪] 武汉轻工大学生命科学与技术学院
关键词:
柚皮素;酿酒酵母;异源合成;同源整合;发酵罐
摘要:
本研究构建酿酒酵母柚皮素细胞合成工厂,优化关键合成步骤,以提升柚皮素产量.采用同源重组技术,在酿酒酵母的rDNA位点构建从对香豆酸到柚皮素的合成途径,包括4-香豆酸辅酶A连接酶(4CL)、查尔酮合成酶(CHS)、查尔酮异构酶(CHI)三个酶基因的完整表达阅读框.同时,单因素法优化YPD发酵培养基中酵母提取物,葡萄糖和胰蛋白胨浓度.结果表明:得到了5株产柚皮素的酿酒酵母改造菌株S1、S2、S3、S4、S5,在4%的酵母提取物,10%的胰蛋白胨和2%的葡萄糖的最优配比下,5 L发酵罐扩大培养,通过分批补料发酵,最高柚皮素产量为438 mg/L,成功实现了在酿酒酵母中异源合成柚皮素途径的构建与优化,为工业化微生物合成柚皮素提供了新的途径和参考价值.
语种:
中文
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高脂饮食及昼夜颠倒对小鼠脾脏免疫功能的影响
作者:
余佳乐;邱熠;周文汀;刘志国
期刊:
武汉轻工大学学报 ,2023年42(03):46-51 ISSN:2095-7386
作者机构:
[周文汀; 余佳乐; 刘志国; 邱熠] 武汉轻工大学 生命科学与技术学院,武汉 432023
关键词:
节律;高脂饮食;免疫;流式
摘要:
探究高脂饮食以及昼夜节律颠倒对于小鼠脾脏免疫功能的影响。将6~7周龄雄性C57BL/6J小鼠分为正常膳食组(CON)和高脂膳食组(HFD),每组根据进食时间又分为白天进食(D)和夜间进食(N),总计4组,每组10只,即:...展开更多 探究高脂饮食以及昼夜节律颠倒对于小鼠脾脏免疫功能的影响。将6~7周龄雄性C57BL/6J小鼠分为正常膳食组(CON)和高脂膳食组(HFD),每组根据进食时间又分为白天进食(D)和夜间进食(N),总计4组,每组10只,即:颠倒饮食组(DC)、白天进食高脂组(DH)、正常节律正常饮食组(NC)和高脂进食组(NH)。小鼠饲养18周后,取脾脏组织及血液分别进行B淋巴细胞分离、流式细胞检测。结果显示:与夜间正常膳食组小鼠脾脏系数0.37相比,颠倒饮食组小鼠脾脏指数为0.4,高脂进食组小鼠脾脏指数为0.41,指数增加,流式细胞检测结果显示正常节律正常饮食组小鼠脾脏B细胞水平显著高于颠倒饮食组小鼠脾脏B细胞水平(P<0.001),正常节律正常饮食组小鼠脾脏B细胞水平也显著高于高脂进食组小鼠脾脏B细胞水平(P<0.01),提示昼夜颠倒饮食以及高脂饮食均会导致小鼠免疫系统发生紊乱。因此,颠倒饮食和高脂饮食小鼠B淋巴细胞水平均显示节律性波动,但和正常小鼠相比,波动性较为紊乱或颠倒。这些结果表明,高脂饮食以及颠倒饮食均会导致B淋巴细胞水平的降低和免疫功能的损害,对免疫系统产生不利影响。收起
语种:
中文
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三丁酸甘油酯的微胶囊化制备工艺及优化
作者:
王虎;周文汀;邱熠;余佳乐;何如怡;...
期刊:
武汉轻工大学学报 ,2023年42(02):108-113 ISSN:2095-7386
作者机构:
[王虎; 周文汀; 邱熠; 余佳乐; 何如怡; 刘志国] 武汉轻工大学生命科学与技术学院
关键词:
三丁酸甘油酯;微胶囊;乳化法
摘要:
为了扩展三丁酸甘油酯的应用,采用乳化法制作三丁酸甘油酯微胶囊,探究了海藻酸钠浓度、氯化钙浓度、水油相比对三丁酸甘油酯微胶囊的影响,并采用单因素分析方法和响应面法优化微胶囊的制备工艺。结果显示:三丁酸甘油酯微胶囊的最佳工艺为藻酸钠浓度1.46%、氯化钙浓度0.6%、水油相比1.16,此时包埋率理论上可达92.01%。
语种:
中文
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黄芩发酵对动物肠道炎症的改善作用
作者:
于潇洁;赵丽;刘志国
期刊:
武汉轻工大学学报 ,2022年41(4):35-42 ISSN:2095-7386
作者机构:
武汉轻工大学 生命科学与技术学院,湖北 武汉430023;[刘志国; 于潇洁; 赵丽] 武汉轻工大学
关键词:
红茶菌;黄芩;黄芩发酵液;果蝇;小鼠;肠炎模型
摘要:
为研究红茶菌发酵黄芩(Scutellaria baicalensis Georgi)的抗菌作用和对果蝇及小鼠的细菌性肠道炎症的改善作用,测定黄芩发酵液的OD值、pH值等来确定红茶菌的发酵条件,采用二倍稀释法确定发酵液的最小抑菌浓度,使用金黄色葡萄球菌(Staphylococcus aureus)建立果蝇和小鼠的肠炎模型,通过果蝇存活率、小鼠空肠组织HE的染色情况、Zo-1和Occludin-1连接蛋白的免疫组化情况、小鼠空肠组织的荧光定量分析来验证发酵液的功能.结果显示,黄芩提取物在30℃下经红茶菌发酵84 h后,对2×108 CFU/mL的金黄色葡萄球菌的抑菌效果提升了8倍,喂食5%黄芩发酵液的果蝇生存率高于空白模型组及黄芩提取物组.小鼠肠道的HE染色结果显示,在黄芩发酵液组,小鼠空肠组织的肠绒毛形态较为完整,与模型组有显著差异.ZO-1、Occludin-1连接蛋白的免疫组化评分有显著改善,qPCR检测结果显示黄芩发酵液组的小鼠空肠IL-6 mRNA与TNF-a mRNA表达量均极显著下降(P<0.01).实验表明红茶菌发酵黄芩能明显改善肠炎动物模型的肠道免疫功能,可为红茶菌发酵中药提取物治疗炎症疾病提供理论依据.
语种:
中文
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昼夜节律异常对小鼠脾脏免疫功能的影响
作者:
张世杰;余佳乐;刘志国
期刊:
武汉轻工大学学报 ,2022年41(2):33-37 ISSN:2095-7386
作者机构:
武汉轻工大学 生命科学与技术学院,武汉430023;[张世杰; 余佳乐; 刘志国] 武汉轻工大学
关键词:
节律;铁死亡;免疫力;转录组测序
摘要:
研究昼夜节律异常对小鼠脾脏免疫功能的影响。采用14月龄的节律基因Per1/Per2双敲除(Per1-/-/Per2-/-)雄性C57BL/6小鼠(双敲除组,DKO)与野生型的雄性C57BL/6小鼠(野生组,WT),测量体重、脾脏重量,同时,对脾脏组织进行HE染色、免疫组化、转录组测序以及脾脏B细胞和T细胞的流式细胞检测,分析节律基因敲除所导致的节律异常对小鼠脾脏免疫功能的影响。结果显示:与野生型相比,双敲除组脾脏指数增加,HE染色显示脾脏白髓变薄,动脉周围淋巴鞘消失且边缘区消失;流式细胞检测结果显示,双敲除组的B细胞和T细胞的比例都显著下降。TFR1免疫组化显示DKO小鼠出现铁死亡表现,转录组测序结果显示双敲除组铁死亡途径显著激活,其中参与脂质过氧化的Acsl4、Lpcat3基因表达显著上调,及谷胱甘肽合成的Gclm、Gclc基因表达显著上调。所得结论:Per1/Per2双敲除节律异常小鼠脾脏免疫功能降低,可能涉及铁死亡途径。
语种:
中文
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omega-3 PUFAs Alleviate High-Fat Diet-Induced Circadian Intestinal Microbes Dysbiosis.
作者:
Gui, Lifeng;Chen, Si;Wang, Hualin;Ruan, Mengcheng;Liu, Yang;...
期刊:
MOLECULAR NUTRITION & FOOD RESEARCH ,2019年63(22):e1900492 ISSN:1613-4125
通讯作者:
Liu, Zhiguo
作者机构:
[Wang, Hualin; Gui, Lifeng; Liu, Zhiguo; Li, Na; Ruan, Mengcheng; Chen, Si; Liu, Yang; Zhang, Hongyu] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Liu, Zhiguo] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China.
关键词:
algae oil;circadian rhythm;fish oil;gut microbiota;omega-3 PUFAs
摘要:
<jats:sec><jats:title>Scope</jats:title><jats:p>Published data support that fish oil and algae oil rich in ω‐3 polyunsaturated fatty acids (PUFAs) protect against hyperlipidemia in mice. This study is aimed to explore the effects of fish oil and algae oil on high‐fat diet (HFD) induced circadian intestinal microbes dysregulation.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>Male C57BL/6 mice are randomly divided into four groups, which are fed a normal chow diet (CON), a HFD, a HFD supplemented with fish oil (FO), and a HFD supplemented with algae oil (AO), respectively, for 12 weeks. At the end of the experiment, mice are sacrificed at 12 h intervals with the first one at zeitgeber time 0 (ZT0) and the second at zeitgeber time 12 (ZT12). FO and AO groups ameliorate diet‐induced hyperlipidemia. The relative abundance of certain genera is improved in FO and AO groups according to 16S rRNA gene sequencing. The short‐chain fatty acids (SCFAs) producing bacteria <jats:italic>Butyricimonas</jats:italic> and some of the genera in the <jats:italic>Lachnospiraceae</jats:italic> recover to the normal circadian rhythm in both FO and AO groups.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>The data show that FO and AO alleviate circadian gut microbiota dysregulation in mice caused by HFD, and support the further investigation of ω‐3 PUFAs as a dietary intervention strategy for the prevention of hyperlipidemia.</jats:p></jats:sec>
语种:
英文
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中国红豆杉羟化酶基因TcCYP725A22的亚细胞定位及过表达作用分析
作者:
廖卫芳;付春华;刘志国;缪礼鸿;余龙江
期刊:
生物工程学报 ,2019年35(6):1109-1116 ISSN:1000-3061
作者机构:
武汉轻工大学生物与制药工程学院,湖北武汉,430023;华中科技大学生命科学与技术学院,湖北武汉,430074;[缪礼鸿; 廖卫芳; 刘志国] 武汉轻工大学;[余龙江; 付春华] 华中科技大学
关键词:
紫杉醇;生物合成途径;中国红豆杉;羟化酶;亚细胞定位;过表达
摘要:
抗癌药物紫杉醇生物合成途径中羟化酶的发现是当今研究的热点和难点。文中利用前期分析得到的1 个新的中国红豆杉羟化酶基因TcCYP725A22 (GenBank 登录号:MF448646.1),构建了亚细胞定位载体pCAMIBA1303-TcCYP725A22-EGFP,瞬时侵染洋葱表皮细胞,激光共聚焦显微镜观察结果发现该基因编码蛋白定位在细胞膜。进一步构建了植物表达载体pBI121-TcCYP725A22,经根癌农杆菌Agrobacterium tumefaciens LBA4404 介导转化到中国红豆杉细胞中过表达后,利用荧光定量PCR (qRT-PCR) 和液质联用(LC-MS) 分析TcCYP725A22 过表达对紫杉醇生物合成的影响。结果显示,瞬时转化pBI121-TcCYP725A22 的红豆杉细胞中TcCYP725A22 基因的转录水平明显高于pBI121 空载体对照组。随着TcCYP725A22 基因过表达,几个已知的紫杉醇合成关键酶基因的表达量也都有不同程度的上调,且细胞中各紫杉烷的含量普遍提高。这些结果说明羟化酶基因TcCYP725A22 极有可能参与紫杉醇的生物合成路径。.
语种:
中文
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Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD
作者:
Liu, Yang;Li, Qi;Wang, Hualin
* ;Zhao, Xiuju
( 赵秀举 ) ;Li, Na;...
期刊:
JOURNAL OF NUTRITIONAL BIOCHEMISTRY ,2019年69:53-62 ISSN:0955-2863
通讯作者:
Wang, Hualin
作者机构:
[Zhao, Xiuju; Wang, Hualin; Li, Qi; Liu, Zhiguo; Li, Na; Liu, Yang; Zhang, Hongyu] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China.;[Chen, Guoxun] Univ Tennessee, Dept Nutr, Knoxville, TN 37996 USA.
通讯机构:
[Wang, Hualin] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China.
关键词:
Bile acid metabolism;Bile component;Circadian rhythm;Fish oil;NAFLD
摘要:
Our previous studies have found that fish oil rich in omega-3 polyunsaturated fatty acids (omega-3 PUFA) protects against non-alcoholic fatty liver disease (NAFLD) in mice. This study was aimed to explore the effects of fish oil on high fat diet (HFD)-induced circadian bile composition chaos. Male C57BL/6 mice were randomly divided into three groups, a control group (CON), a HFD group and a fish oil (FO) group, which were fed a normal chow diet, a HFD, and a HFD supplemented with FO, respectively for 12 weeks. At the end of the experiment, liver tissue, blood and bile samples were processed at 12-h intervals with the first one at zeitgeber time 0 (ZT0) and the second at zeitgeber time 12 (ZT12). Metabolites in bile were determined using UPLC-QTOF-MS, screened using multivariate statistical analysis, and analyzed using KEGG database and Metaboanalyst. The expression levels of key proteins in bile acid metabolism were examined using western blot. Results of biochemical analysis and H&E staining illustrated that feeding of HFD induced NAFLD, which was ameliorated in FO group. The bile content of each group at ZT0 (CON, HFD, or FO group) was respectively higher than that at ZT12 (P<.05). The metabolic pathway analysis of differential metabolites showed that these differences were correlated with amino acid metabolism, fatty acid biosynthesis and primary bile acid synthesis at ZT0. FO supplement could modify bile composition, which was related to the influence of its omega-3 PUFA on liver metabolism. omega-3 PUFA may also regulate the circadian rhythm of bile metabolism.
语种:
英文
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Inhibition effects of low concentrations of epigallocatechin gallate on the biofilm formation and hemolytic activity of Listeria monocytogenes
作者:
Du, Wenfang;Zhou, Min;Liu, Zhiguo;Chen, Ying;Li, Rui*
期刊:
Food Control ,2018年85:119-126 ISSN:0956-7135
通讯作者:
Li, Rui
作者机构:
[Du, Wenfang; Zhou, Min; Liu, Zhiguo; Li, Rui] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Chen, Ying] Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China.
通讯机构:
[Li, Rui] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.
关键词:
Biofilm;EGCG;Inhibition;L. monocytogenes;Virulence
摘要:
Listeria monocytogenes (L. monocytogenes) is a well-known food-borne pathogen that causes systemic listeriosis. Its biofilm-forming ability is known to be important for its antimicrobial resistance and persistence. Epigallocatechin-gallate (EGCG) is the highest component of tea polyphenols in tea extracts and has broad-spectrum antimicrobial activities. In this study, the efficacys of EGCG to inhibit biofilm formation and hemolytic activity of L. monocytogenes were determined. EGCG at 20 μg/mL, 40 μg/mL (less than compound's minimum inhibitory concentration, MIC) and 200 μg/mL (1 MIC) were tested. Crystal violet staining showed that sub-MIC and MIC of EGCG could significantly reduce the biofilm formation by L. monocytogenes on polystyrene microtiter plates at three temperatures (15 °C, 30 °C and 37 °C). EGCG reduced the sessile cell numbers present in biofilm at those temperatures. EGCG also significantly inhibited hemolytic activity of L. monocytogenes as measured by sheep red blood cells. Real time PCR assay was used to investigate the relative gene expression of L. monocytogenes grown at 37 °C and revealed that EGCG down-regulated virulence genes (inlA and hly), SOS response genes (recA and yneA) and quorum sensing gene (agrA). These results suggested the feasibility of using EGCG in food industry to control L. monocytogenes biofilm formation. © 2017 Elsevier Ltd
语种:
英文
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Control of Listeria monocytogenes biofilm by paenibacterin, a natural antimicrobial lipopeptide
作者:
Li, Rui* ;Du, Wenfang;Yang, Jun;Liu, Zhiguo;Yousef, Ahmed E.*
期刊:
Food Control ,2018年84:529-535 ISSN:0956-7135
通讯作者:
Li, Rui;Yousef, Ahmed E.
作者机构:
[Du, Wenfang; Yang, Jun; Li, Rui; Liu, Zhiguo] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Yousef, Ahmed E.] Ohio State Univ, Dept Food Sci & Technol, 2015 Fyffe Rd, Columbus, OH 43210 USA.
通讯机构:
[Li, Rui] W;[Yousef, Ahmed E.] O;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;Ohio State Univ, Dept Food Sci & Technol, 2015 Fyffe Rd, Columbus, OH 43210 USA.
关键词:
Biofilm;Fluorescent microscopy;Gene expression;Listeria monocytogenes;Paenibacterin
摘要:
Paenibacterin is a novel antimicrobial lipopeptide produced by Paenibacillus thiaminolyticus. The present study assesses the efficacy of paenibacterin in inhibiting Listeria monocytogenes biofilm formation and removing established biofilm. Paenibacterin at 1.7 μg/mL (less than compound's minimum inhibitory concentration, MIC) and higher concentrations (3.4 μg/mL, 6.8 μg/mL) were tested. Concentrations greater than the lipopeptide's MIC significantly inhibited the formation of L. monocytogenes biofilm on polystyrene microtiter plates at 30 °C and 37 °C. Paenibacterin also was added to established biofilm and its structure or removal was monitored by fluorescence microscopes after appropriate staining. Results show that paenibacterin could eliminate established biofilms formed at 30 °C for 72 h, whereas it could not disrupt stronger biofilms formed at 37 °C for 72 h. Motility of L. monocytogenes is important for its ability to form biofilm. Swimming assay confirmed that paenibacterin suppressed L. monocytogenes motility. Real time quantitative PCR data revealed that paenibacterin down-regulated L. monocytogenes critical biofilm-associated genes, prfA, agrA, flaA, fliG and flgE. These results suggested the feasibility of using paenibacterin in food processing environments to control L. monocytogenes growth and biofilm formation, or even for removal of some established biofilms. © 2017 Elsevier Ltd
语种:
英文
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Effects of exposure of adult mice to multi-walled carbon nanotubes on the liver lipid metabolism of their offspring
作者:
Zhang, Hong-yu;Chen, Ru-long;Shao, Yang;Wang, Hua-lin;Liu, Zhi-guo*
期刊:
TOXICOLOGY RESEARCH ,2018年7(5):809-816 ISSN:2045-452X
通讯作者:
Liu, Zhi-guo
作者机构:
[Wang, Hua-lin; Liu, Zhi-guo; Zhang, Hong-yu; Shao, Yang; Chen, Ru-long] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 Xuefu Southern Rd, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Liu, Zhi-guo] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 Xuefu Southern Rd, Wuhan 430023, Hubei, Peoples R China.
摘要:
Objective: To explore the toxicity of multi-walled carbon nanotubes (MWCNTs) on the liver lipid metabolism of offspring mice and the possible mechanisms involved. Method: Virgin female (16–18 g) and male (18–20 g) C57BL/6 mice were randomly divided into two groups: Control group and Test group. After anesthesia with chloral hydrate, the mice were administered 50 μL saline or dust solution by intratracheal instillation (Control group: 50 μL saline; Test group: 15 mg kg−1 MWCNTs). Mice were injected with these doses once a week for 13 weeks. Then, male and female mice in the same group were allowed to mate to produce offspring. The pups were fed with normal diet until the end of the experiment (12 weeks old). The offspring mice were sacrificed by decapitation to detect the blood biochemistry and the expression of genes and proteins. Results: Compared with the Control group, MWCNTs significantly reduced the weight of offspring mice (male and female) and led to histopathological changes in the liver tissues. The expression of liver fat synthesis gene significantly increased (P < 0.05 or P < 0.01). The expression of genes and proteins involved in the inflammatory reactions appeared to be abnormal (P < 0.05 or P < 0.01). Conclusion: Exposure of adult mice to MWCNTs can affect the expression of fatty acid synthesis genes in the liver tissues of offspring mice, leading to disruption of liver function and accumulation of lipid droplets in the hepatocytes. The imbalance between M1 and M2 liver macrophage phenotypes may be one of the underlying mechanisms of action of MWCNTs leading to disordered fatty acid synthesis in offspring mice.
Objective: To explore the toxicity of multi-walled carbon nanotubes (MWCNTs) on the liver lipid metabolism of offspring mice and the possible mechanisms involved.
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英文
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Fish Oil Ameliorates High-Fat Diet Induced Male Mouse Reproductive Dysfunction via Modifying the Rhythmic Expression of Testosterone Synthesis Related Genes
作者:
Wang, Hualin;Cai, Yazheng;Shao, Yang;Zhang, Xifeng;Li, Na;...
期刊:
International Journal of Molecular Sciences ,2018年19(5):1325 ISSN:1422-0067
通讯作者:
Liu, Zhiguo
作者机构:
[Wang, Hualin; Liu, Zhiguo; Li, Na; Shao, Yang; Zhang, Xifeng; Zhang, Hongyu; Cai, Yazheng] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 30023, Hubei, Peoples R China.
通讯机构:
[Liu, Zhiguo] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 30023, Hubei, Peoples R China.
关键词:
high-fat diet;fish oil;reproduction function;circadian rhythms
摘要:
The present study aims to investigate the protective effects of ω-3 polyunsaturated fatty acids (ω-3PUFAs) against high-fat diet induced male mouse reproductive dysfunction and to explore circadian regulation mechanisms. Male C57BL/6 mice were randomly divided into three groups and fed a normal chow diet (control group, CON), a high-fat diet (HFD group) or a HFD supplemented with fish oil (FO group) for 12 weeks. After 12 weeks of feeding, the body weight and the ratio of perinephric and epididymal fat weight to body weight were significantly higher in the HFD group compared with the CON group. The supplement of fish oil rich in ω-3PUFAs only slightly reduced the HFD-induced obesity but remarkably ameliorated HFD-induced dyslipidemia, sexual hormones disorder, testicle lesions and germ cell apoptosis. Fish oil supplementation restored the expression of steroid synthesis associated genes in HFD fed mouse and flattened the HFD-induced oscillations in circadian genes’ expression. Fish oil supplementation prevented HFD-induced male mouse reproductive dysfunction and modified the rhythmic expression of testosterone synthesis related genes. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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英文
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Dynamic lipid profile of hyperlipidemia mice
作者:
Chen, Yu-Lian;Xiao, Chuan-Hao;Hu, Zhi-Xiong;Liu, Xiao-Shan;Liu, Zhiguo;...
期刊:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2017年1055-1056:165-171 ISSN:1570-0232
通讯作者:
Zhao, Xiu-Ju
( 赵秀举 ) ;Zhang, Wei-Nong
作者机构:
[Zhao, Xiu-Ju; Hu, Zhi-Xiong; Liu, Zhiguo; Liu, Xiao-Shan; Chen, Yu-Lian] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Zhang, Wei-Nong] Wuhan Polytech Univ, Sch Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;[Xiao, Chuan-Hao] Puyang Vocat & Tech Coll, Puyang 457000, Peoples R China.
通讯机构:
[Zhao, Xiu-Ju; Zhang, Wei-Nong] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Sch Food Sci & Engn, 68 South Xuefu Rd, Wuhan 430023, Peoples R China.
关键词:
Biomarkers of serum fatty acids;GC-MS;Hyperlipidemia
摘要:
Biomarkers of serum fatty acids in hyperlipidemia need to be elucidated. 90 SPF KM male mice were randomly divided into 18 groups (n = 5/group), control groups, and high fat diet (HFD) groups at 9 time points. On day 7, 10, 15, 18, 21, 24, 28, 31, and 35, the mice were sacrificed;blood was collected into tubes from the eyes, serum samples for clinical biochemistry assays and gas chromatography–mass spectroscopy were attained after centrifugation, and the contents of serum fatty acids were detected with GC–MS. Sections of livers were taken and stored in formalin solution for histological assessments. No species differences existed in all these groups. The contents of C16:1, C18:1, C22:6 were significantly different between HFD groups and the corresponding controls;meanwhile, the proportion of fatty acids, especially the monounsaturated degree, the polyunsaturated degree, changed significantly and regularly (P < 0.05). Thus the three unsaturated fatty acids C16:1, C18:1, C22:6 and the monounsaturated/polyunsaturated unsaturated degrees may be as potential biomarkers of hyperlipidemia. ©2017 Elsevier B.V.
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英文
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Comparative proteomic analysis of fibrotic liver of rats fed high fat diet contained lard versus corn oil
作者:
Wang, Hualin;Sit, Wat-Hung;Tipoe, George Lim;Liu, Zhiguo;Wan, Jennifer Man-Fan*
期刊:
Clinical Nutrition ,2017年36(1):198-208 ISSN:0261-5614
通讯作者:
Wan, Jennifer Man-Fan
作者机构:
[Wang, Hualin; Liu, Zhiguo] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.;[Wang, Hualin; Wan, Jennifer Man-Fan; Sit, Wat-Hung] Univ Hong Kong, Sch Biol Sci, Food & Nutr Div, Hong Kong, Hong Kong, Peoples R China.;[Tipoe, George Lim] Univ Hong Kong, Li Ka Shing Fac Med, Dept Anat, Hong Kong, Hong Kong, Peoples R China.
通讯机构:
[Wan, Jennifer Man-Fan] U;Univ Hong Kong, Sch Biol Sci, Food & Nutr Div, Hong Kong, Hong Kong, Peoples R China.
关键词:
Carbon tetrachloride;Corn oil;Lard;Liver fibrosis;Proteomics
摘要:
Introduction The influences of dietary fatty acids on the progress of chronic liver diseases have attracted lots of attentions, but the mechanisms of the effects of lipids rich in saturated fatty acids or PUFAs on hepatic fibrogenesis remain unclear. Methods Female Fischer 344 rats were fed normal chow or chow plus 20% (w/w) of corn oil or lard, respectively, and injected CCl4 twice a week for 4 weeks to induce liver fibrosis. Masson's staining was adopted to illustrate the fibrosis level. The mRNA expression level of α-SMA and the DNA methylation level of its promoter region were analyzed. A 2-DE gel based proteomic approach was constructed to investigate the differential expression level of hepatic proteome between three diet groups. Results Histological evaluations and α-SMA expression analysis illustrated the high corn oil intake has no effects on hepatic fibrogenesis, but lard intake aggravated liver fibrosis, partly attributed to DNA demethylation of α-SMA promoter region. 2-DE Gel based proteomic study demonstrated excessive lard consumption elevated the expression of fibrosis related alpha-1-antitrypsin precursor, and endoplasmic reticulum stress related proteins such as heat shock cognate 71 kDa, eukaryotic translation initiation factor 4A1 and protein disulfide isomerase associated 3. Moreover, unlike corn oil rich in PUFAs, lard had no effects to elevate the expression of glutathione S-transferases, but decreased the expression of iron store related proteins heme binding protein 1 and ferritin. Conclusions Lard intake aggravates CCl4 induced liver fibrosis via enhancing the expression of fibrogenesis and ER stress related proteins, and disturbing the hepatic transmethylation reaction. © 2015 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism
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英文
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ER stress-mediated cell damage contributes to the release of EDA(+) fibronectin from hepatocytes in nonalcoholic fatty liver disease
作者:
He, Lei* ;Yuan, Fa-hu;Chen, Ting;Huang, Qiang;Wang, Yu;...
期刊:
当代医学科学 ,2017年37(2):217-225 ISSN:2096-5230
通讯作者:
He, Lei;Liu, Zhi-guo
作者机构:
[He, Lei] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Blood Transfus, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Yuan, Fa-hu] Jianghan Univ, Sch Med, Wuhan 430000, Peoples R China.;[Huang, Qiang; Chen, Ting; Liu, Zhi-guo] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Wang, Yu] Huazhong Univ Sci & Technol, Sch Basic Med, Dept Biochem & Mol Biol, Tongji Med Coll, Wuhan 430030, Peoples R China.
通讯机构:
[He, Lei] H;[Liu, Zhi-guo] W;Huazhong Univ Sci & Technol, Tongji Hosp, Dept Blood Transfus, Tongji Med Coll, Wuhan 430030, Peoples R China.;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
关键词:
fibronectin;ER stress;nonalcoholic fatty liver disease;necrosis
摘要:
Fibronectin containing extra domain A (EDA+ FN), a functional glycoprotein participating in several cellular processes, correlates with chronic liver disease. Herein, we aim to investigate the expression and secretion of EDA+ FN from hepatocytes in nonalcoholic fatty liver disease (NAFLD) and the underlying mechanisms. Circulating levels of EDA+ FN were determined by ELISA in clinical samples. Western blotting and flow cytometry were performed on L02 and HepG2 cell lines to analyze whether the levels of EDA+ FN were associated with endoplasmic reticulum (ER) stress-related cell death. Circulating levels of EDA+ FN in NAFLD patients were significantly higher than those in control subjects, and positively related with severity of ultrasonographic steatosis score. In cultured hepatocytes, palmitate up-regulated the expression of EDA+ FN in a dose-dependent manner. Conversely, when the cells were pretreated with 4-phenylbutyrate, a specific inhibitor of ER stress, up-regulation of EDA+ FN could be abrogated. Moreover, silencing CHOP by shRNA enhanced the release of EDA+ FN from hepatocytes following palmitate treatment, which was involved in ER stress-related cell damage. These findings suggest that the up-regulated level of EDA+ FN is associated with liver damage in NAFLD, and ER stress-mediated cell damage contributes to the release of EDA+ FN from hepatocytes. © 2017, Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg.
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英文
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Fish oil feeding modulates the expression of hepatic MicroRNAs in a western-style diet-induced nonalcoholic fatty liver disease rat model
作者:
Wang, Hualin;Shao, Yang;Yuan, Fahu;Feng, Han;Li, Na;...
期刊:
BioMed Research International ,2017年2017:1-11 ISSN:2314-6133
通讯作者:
Liu, Zhiguo
作者机构:
[Wang, Hualin; Feng, Han; Yuan, Fahu; Liu, Zhiguo; Li, Na; Shao, Yang; Zhang, Hongyu] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.;[Yuan, Fahu] Jianghan Univ, Sch Med, Wuhan 430000, Hubei, Peoples R China.;[Wu, Chaodong] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA.
通讯机构:
[Liu, Zhiguo] W;Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.
摘要:
Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver diseases worldwide. Recent studies have indicated that fish oil supplementation has benefits against NAFLD. Our previous transcriptomic study has validated the effect of fish oil supplementation on altering hepatic gene expression in a NAFLD rat model. In the current study, we examined the effects of fish oil on the expression of hepatic microRNAs. Male Sprague-Dawley rats were fed with a lab chow (CON), high-fat high-cholesterol diet (WD), or WD supplemented with fish oil (FOH), respectively. Small RNAs were extracted from livers for RNA-sequencing. A total of 79 miRNAs were identified as differentially expressed miRNAs (DEMs) between FOH and WD groups, exemplified by rno-miR-29c-3p, rno-miR-30d-5p, rno-miR-33-5p, rno-miR-34a, and rno-miR-328a-3p. Functional annotation of DEMs predicted target genes suggested that the altered hepatic miRNAs contributed to fish oil modification of hepatic lipid metabolism and signaling transduction. Integrative analysis of DEMs and differentially expressed genes suggested that the expression difference of Pcsk9, Insig2, Per3, and Socs1/3 between FOH and WD groups may be due to miRNA modification. Our study reveals that fish oil supplementation alters hepatic expression of miRNAs, which may contribute to fish oil amelioration of NAFLD in rats. © 2017 Hualin Wang et al.
语种:
英文
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Intervention of pumpkin seed oil on metabolic disease revealed by metabonomics and transcript profile
作者:
Zhao, Xiu-Ju
* ( 赵秀举 ) ;Chen, Yu-Lian;Fu, Bing;Zhang, Wen;Liu, Zhiguo
* ;...
期刊:
Journal of the Science of Food and Agriculture ,2017年97(4):1158-1163 ISSN:0022-5142
通讯作者:
Zhao, Xiu-Ju
( 赵秀举 ) ;Liu, Zhiguo
作者机构:
[Zhao, Xiu-Ju; Liu, Zhiguo; Chen, Yu-Lian] Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.;[Zhao, Xiu-Ju] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr Oil, Wuhan 430062, Peoples R China.;[Fu, Bing] Changyuan Cuisine Vocat & Tech Coll, Changyuan 453400, Peoples R China.;[Zhang, Wen] Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China.;[Zhuo, Hexian] Xinxiang Inst Drug Control Food & Drug Adm Xinxia, 17 Jiankang Rd, Xinxiang 453000, Peoples R China.
通讯机构:
[Zhao, XJ; Liu, ZG] W;Wuhan Polytech Univ, Collaborat Innovat Ctr Proc Agr Prod Hubei Prov, Sch Biol & Pharmaceut Engn, 68 South Xuefu Rd,Changqing Garden, Wuhan 430023, Peoples R China.
关键词:
pumpkin seed oil (PSO);metabolic disease (MD);metabonomics;transcript profile
摘要:
<jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p>Understanding the metabolic and transcription basis of pumpkin seed oil (<jats:styled-content style="fixed-case">PSO</jats:styled-content>) intervention on metabolic disease (<jats:styled-content style="fixed-case">MD</jats:styled-content>) is essential to daily nutrition and health.</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>This study analyzed the liver metabolic variations of Wistar rats fed normal diet (<jats:styled-content style="fixed-case">CON</jats:styled-content>), high‐fat diet (<jats:styled-content style="fixed-case">HFD</jats:styled-content>) and high‐fat plus <jats:styled-content style="fixed-case">PSO</jats:styled-content> diet (<jats:styled-content style="fixed-case">PSO</jats:styled-content>) to establish the relationship between the liver metabolite composition/transcript profile and the effects of <jats:styled-content style="fixed-case">PSO</jats:styled-content> on <jats:styled-content style="fixed-case">MD</jats:styled-content>. By using proton nuclear magnetic resonance spectroscopy together with multivariate data analysis, it was found that, compared with <jats:styled-content style="fixed-case">CON</jats:styled-content> rats, <jats:styled-content style="fixed-case">HFD</jats:styled-content> rats showed clear dysfunctions of choline metabolism, glucose metabolism and nucleotide and amino acid metabolism. Using quantitative real‐time polymerase chain reaction (<jats:styled-content style="fixed-case">qPCR</jats:styled-content>), it was found that, compared with <jats:styled-content style="fixed-case">HFD</jats:styled-content> rats, <jats:styled-content style="fixed-case">PSO</jats:styled-content> rats showed alleviated endoplasmic reticulum stress accompanied by lowered unfolded protein response.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSION</jats:title><jats:p>These findings provide useful information to understand the metabolic alterations triggered by <jats:styled-content style="fixed-case">MD</jats:styled-content> and to evaluate the effects of <jats:styled-content style="fixed-case">PSO</jats:styled-content> intervention. © 2016 Society of Chemical Industry</jats:p></jats:sec>
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英文
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Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches
作者:
Zhang, Xi-Feng;Liu, Zhi-Guo;Shen, Wei;Gurunathan, Sangiliyandi*
期刊:
International Journal of Molecular Sciences ,2016年17(9):1534 ISSN:1661-6596
通讯作者:
Gurunathan, Sangiliyandi
作者机构:
[Liu, Zhi-Guo; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao 266109, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.
通讯机构:
[Gurunathan, Sangiliyandi] K;Konkuk Univ, Dept Stem Cell & Regenerat Biotechnol, Seoul 143701, South Korea.
关键词:
silver nanoparticles;synthesis;characterization;applications;mechanisms;cancer therapy
摘要:
Recent advances in nanoscience and nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials among several metallic nanoparticles that are involved in biomedical applications. AgNPs play an important role in nanoscience and nanotechnology, particularly in nanomedicine. Although several noble metals have been used for various purposes, AgNPs have been focused on potential applications in cancer diagnosis and therapy. In this review, we discuss the synthesis of AgNPs using physical, chemical, and biological methods. We also discuss the properties of AgNPs and methods for their characterization. More importantly, we extensively discuss the multifunctional bio-applications of AgNPs; for example, as antibacterial, antifungal, antiviral, anti-inflammatory, anti-angiogenic, and anti-cancer agents, and the mechanism of the anti-cancer activity of AgNPs. In addition, we discuss therapeutic approaches and challenges for cancer therapy using AgNPs. Finally, we conclude by discussing the future perspective of AgNPs. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
语种:
英文
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Cytotoxic effects of ZnO nanoparticles on mouse testicular cells
作者:
Han, Zhe;Yan, Qi;Ge, Wei;Liu, Zhi-Guo;Gurunathan, Sangiliyandi;...
期刊:
INTERNATIONAL JOURNAL OF NANOMEDICINE ,2016年11:5187-5203 ISSN:1178-2013
通讯作者:
Zhang, Xi-Feng
作者机构:
[Yan, Qi; Liu, Zhi-Guo; Han, Zhe; Zhang, Xi-Feng] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Ge, Wei; Shen, Wei] Qingdao Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Reprod & Germplasm Enhancement Univ, Qingdao, Peoples R China.;[Gurunathan, Sangiliyandi] Konkuk Univ, Dept Stem Cell & Regenerat Biol, Seoul, South Korea.;[De Felici, Massimo] Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.
通讯机构:
[Zhang, Xi-Feng] W;Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
关键词:
ZnO nanoparticle;Sertoli cells;Leydig cells;mice
摘要:
Cytotoxic effects of ZnO nanoparticles on mouse testicular cells Zhe Han,1,* Qi Yan,1,* Wei Ge,2 Zhi-Guo Liu,1 Sangiliyandi Gurunathan,3 Massimo De Felici,4 Wei Shen,2 Xi-Feng Zhang1 1College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China; 2Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, People’s Republic of China; 3Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea; 4Department of Biomedicine and Prevention, University of Rome “Tor Vergata”, Rome, Italy *These authors contributed equally tothis work Background: Nanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model. Methods: ZnO NPs with a crystalline size of 70 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and atomic force microscopy. The cytotoxicity of the ZnO NPs was examined in vitro on Leydig cell and Sertoli cell lines, and in vivo on the testes of CD1 mice injected with single doses of ZnO NPs.Results: ZnO NPs were internalized by Leydig cells and Sertoli cells, and this resulted in cytotoxicity in a time- and dose-dependent manner through the induction of apoptosis. Apoptosis likely occurred as a consequence of DNA damage (detected as γ-H2AX and RAD51 foci) caused by increase in reactive oxygen species associated with loss of mitochondrial membrane potential. In addition, injection of ZnO NPs in male mice caused structural alterations in the seminiferous epithelium and sperm abnormalities.Conclusion: These results demonstrate that ZnO NPs have the potential to induce apoptosis in testicular cells likely through DNA damage caused by reactive oxygen species, with possible adverse consequences for spermatogenesis and therefore, male fertility. This suggests that evaluating the potential impacts of engineered NPs is essential prior to their mass production, to address both the environmental and human health concerns and also to develop sustainable and safer nanomaterials. Keywords: ZnO nanoparticle, Sertoli cells, Leydig cells, mice
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英文
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Molecular screening and characterization of Shiga toxin-producing Escherichia coli in retail foods
作者:
Li, Rui;Tan, Xiao;Xiao, Jie;Wang, Hongxun;Liu, Zhiguo;...
期刊:
Food Control ,2016年60:180-188 ISSN:0956-7135
通讯作者:
Miyamoto, Takahisa
作者机构:
[Xiao, Jie; Wang, Hongxun; Tan, Xiao; Zhou, Min; Bi, Wanglai; Li, Rui; Liu, Zhiguo] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Miyamoto, Takahisa] Kyushu Univ, Dept Biosci & Biotechnol, Lab Food Hyg Chem, Fukuoka 8128581, Japan.;[Miyamoto, Takahisa] Kyushu Univ, Dept Biosci & Biotechnol, Lab Food Hyg Chem, Higashi Ku, 6-10-1 Hakozaki, Fukuoka 8128581, Japan.
通讯机构:
[Miyamoto, Takahisa] K;Kyushu Univ, Dept Biosci & Biotechnol, Lab Food Hyg Chem, Higashi Ku, 6-10-1 Hakozaki, Fukuoka 8128581, Japan.
关键词:
Characterization;Culture isolation;PCR screening;Retail foods;STEC
摘要:
Shiga toxin-producing Escherichia coli (STEC) strains are one of the most important recently emerged groups of food borne pathogens. This study investigated the prevalence of molecular markers for STEC and characteristics of E. coli O157 isolates from foods sold at retail markets in Wuhan, China. A total of 489 samples (350 meat products and 139 raw vegetables) were purchased from 22 large scale markets between July of 2011 and September of 2013. The meat samples consisted of frozen chicken products, raw pork, raw beef, frozen fish products and processed duck products. The raw vegetable samples consisted of lettuce, bok choy, radish, spinach, cucumber, and tomato. Shiga toxin genes (stx1 and stx2) and an O-group marker of the seven main pathogenic STEC serogroups (O157, O26, O45, O103, O111, O121, and O145) were detected in the samples by using PCR. 100% agreement was obtained between the results of the PCR targeting for wzyO157 and the PCR targeting for rfbEO157 gene. The result demonstrated that PCR assay targeting for wzyO157 gene can be employed as an effective screening method for E. coli O157 in food sample. In the study, E. coli O157 and non-O157 STEC were detected in 55 (11.2%) and 75 (15.3%) samples by PCR screening, respectively. There was significant difference in the occurrence of STEC contamination between supermarkets (19/127, 15.0%) and open markets (111/362, 30.7%) (P < 0.05). Out of 489 samples, 5 samples carried O45, 1 sample carried O145 and 1 sample carried O111. Markers for O103, O26 and O121 were not detected. This result differed from other reports. Immunomagnetic separation based cultivation technique was used to isolate E. coli O157 from 27 food samples collected in 2013. Finally 7 E. coli O157 isolates were obtained. Among the 7 isolates, the prevalent stx genotype was stx1a and stx2a. Four E. coli O157 strains exhibited toxic effects on Vero cells, while 3 isolates had no detectable cytotoxicity effects even though they contained stx genes. All E. coli O157 isolates were sensitive to the 12 antimicrobials tested except for roxithromycin. There are some inconsistencies between the PCR screening and culture results. Characteristics of STEC isolates should be evaluated and considered for monitoring STEC contamination in foods. © 2015 Elsevier Ltd.
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英文
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