Geographical discrimination and adulteration analysis for edible oils using two-dimensional correlation spectroscopy and convolutional neural networks (CNNs)
作者：
Liu, Yan* ;Yao, Liyun;Xia, Zhenzhen;Gao, Yonggui;Gong, Zhiyong
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2021年246:118973 ISSN：1386-1425
通讯作者：
Liu, Yan
作者机构：
[Liu, Yan; Gao, Yonggui; Gong, Zhiyong; Yao, Liyun] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Peoples R China.;[Liu, Yan; Gao, Yonggui; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430064, Peoples R China.;[Liu, Yan; Gao, Yonggui; Gong, Zhiyong] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Convolutional neural networks;Edible oils;Near infrared spectroscopy;Two-dimensional correlation spectroscopy
摘要：
Geographical discrimination and adulteration analysis play significant roles in edible oil analysis. A novel method for discrimination and adulteration analysis of edible oils were proposed in this study. The two-dimensional correlation spectra of edible oils were obtained by solvents perturbation and the convolutional neural networks (CNNs) were constructed to analyze the synchronous and asynchronous correlation spectra of the edible oils. The differences for geographical origins of oils or oil types could be amplificated through the networks. For different networks, the layer sequences and the filter number of convolutional layers may affect the analysis results. A group of sesame oils from different geographical origins and a group of olive oils adulterated by other vegetable oils were adopted to evaluate the proposed method. The results show that the proposed method may provide an alternative method for edible oil discrimination and adulteration analysis in practical applications. For the two datasets, the prediction accuracy could be 97.3% and 88.5%, respectively. (C) 2020 Elsevier B.V. All rights reserved.
语种：
英文
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Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure
作者：
Liu, Zhezhe;Zhao, Xiaoxue;Wu, Libiao;Zhou, Shuang* ;Gong, Zhiyong;...
期刊：
TOXINS ,2020年12(3):193- ISSN：2072-6651
通讯作者：
Zhou, Shuang;Wu, Yongning
作者机构：
[Gong, Zhiyong; Wu, Libiao; Liu, Zhezhe] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wu, Yongning; Zhao, Xiaoxue; Zhao, Yunfeng; Zhou, Shuang; Wu, YN; Liu, Zhezhe] Chinese Acad Med Sci, China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Food Safety Res Unit 2019RU014, Beijing 100021, Peoples R China.
通讯机构：
[Zhou, S; Wu, YN] C;Chinese Acad Med Sci, China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Food Safety Res Unit 2019RU014, Beijing 100021, Peoples R China.
关键词：
UHPLC-MS/MS;biomarkers;emerging mycotoxins;human biomonitoring;metabolites;urine
摘要：
A variety of mycotoxins from different sources frequently contaminate farm products, presenting a potential toxicological concern for animals and human. Mycotoxin exposure has been the focus of attention for governments around the world. To date, biomarkers are used to monitor mycotoxin exposure and promote new understanding of their role in chronic diseases. The goal of this research was to develop and validate a sensitive UHPLC-MS/MS method using isotopically-labeled internal standards suitable for accurate determination of 18 mycotoxin biomarkers, including fumonisins, ochratoxins, Alternaria and emerging Fusarium mycotoxins (fumonisin B1, B2, and B3, hydrolyzed fumonisin B1 and B2, ochratoxin A, B, and alpha, alternariol, alternariol monomethyl ether, altenuene, tentoxin, tenuazonic acid, beauvericin, enniatin A, A1, B, and B1) in human urine. After enzymatic digestion with β-glucuronidase, human urine samples were cleaned up using HLB solid phase extraction cartridges prior to instrument analysis. The multi-mycotoxin and analyte-specific method was validated in-house, providing satisfactory results. The method provided good linearity in the tested concentration range (from LOQ up to 25–500 ng/mL for different analytes), with R2 from 0.997 to 0.999. The limits of quantitation varied from 0.0002 to 0.5 ng/mL for all analytes in urine. The recoveries for spiked samples were between 74.0% and 133%, with intra-day precision of 0.5%–8.7% and inter-day precision of 2.4%–13.4%. This method was applied to 60 urine samples collected from healthy volunteers in Beijing, and 10 biomarkers were found. At least one biomarker was found in all but one of the samples. The high sensitivity and accuracy of this method make it practical for human biomonitoring and mycotoxin exposure assessment. © 2020 by the authors.
语种：
英文
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QuEChERS-HPLC-Q-TOF/MS快速筛查蔬菜中32种农药残留
作者：
杨明;涂凤琴;王冬梅;伊鋆;江小明;...
期刊：
食品工业 ,2020年41(4):289-293 ISSN：1004-471X
作者机构：
[江小明; 杨明; 王冬梅; 涂凤琴; 伊鋆] 武汉食品化妆品检验所;武汉轻工大学食品科学与工程学院;[宫智勇] 武汉轻工大学
关键词：
高效液相色谱-四极杆-飞行时间质谱法;农药残留;蔬菜
摘要：
建立了QuEChERS-高效液相色谱-四极杆-飞行时间质谱法用于蔬菜中32种农药残留的快速筛查与确证分析方法。乙睛为提取剂,氯化钠盐析, C18和PSA为净化剂,电喷雾正离子扫描。通过TOF MS-IDA-MS/MS扫描模式,一次采集即可同时获得农药分子的一级精确质量数和二级质谱图信息。在线性范围内32种化合物线性关系良好,相关系数均大于0.999 0,在三个加标水平（1, 4和20 LOQ）下的平均回收率为70.41%～103.1%,相对标准偏差为1.2%～11.2%,方法检出限LOD为0.3～1.3μg/kg,定量限LOQ为1.0～4.0μg/kg。与国标相比,大大缩短前处理时间;此外,该方法中吡虫啉、虫酰肼等9种农药的方法检出限得到了有效降低;除乙霉威和嘧菌酯回收率略低,其它30种农药回收率均无明显差别。该方法简单快速、高效准确、灵敏度高,能够快速筛查和分析蔬菜中残留农药。
语种：
中文
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Natural Occurrence of Deoxynivalenol and Its Acetylated Derivatives in Chinese Maize and Wheat Collected in 2017
作者：
Yan, Pianpian;Liu, Zhezhe;Liu, Shiqiao;Yao, Liyun;Liu, Yan;...
期刊：
TOXINS ,2020年12(3):200- ISSN：2072-6651
通讯作者：
Gong, Zhiyong
作者机构：
[Liu, Yan; Yan, Pianpian; Liu, Shiqiao; Gong, Zhiyong; Yao, Liyun; Liu, Zhezhe] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;[Wu, Yongning] Minist Hlth, Key Lab Food Safety Risk Assessment, China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China.
通讯机构：
[Gong, Zhiyong] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.
关键词：
deoxynivalenol;3-acetyl-deoxynivalenol;15-acetyl-deoxynivalenol;co-occurrence mycotoxins;wheat and maize;UHPLC-MS;MS
摘要：
Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. In this study, 579 wheat samples and 606 maize samples intended for consumption were collected from China in 2017 and analyzed to determine the co-occurrence of type-B trichothecenes (DON, 3-ADON, and 15-ADON). All the wheat samples tested positive for DON, while 99.83% of the maize samples were DON-positive with mean DON concentrations of 165.87 and 175.30 µg/kg, respectively. Per the Chinese standard limits for DON, 3.63% of wheat and 2.97% of the maize samples were above the maximum limit of 1000 µg/kg. The DON derivatives (3-ADON and 15-ADON) were less frequently found and were present at lower levels than DON in wheat. 3-ADON and 15-ADON had incidences of 13.53% and 76.40%, respectively, in maize. By analyzing the distribution ratio of DON and its derivatives in wheat and maize, DON (95.51%) was the predominant toxin detected in wheat samples, followed by 3.97% for the combination of DON + 3-ADON, while DON + 3-ADON + 15-ADON and DON + 15-ADON were only found in 0.17% and 0.35% of wheat samples, respectively. Additionally, a large amount of the maize samples were contaminated with DON + 15-ADON (64.19%) and DON (22.11%). The samples with a combination of DON + 3-ADON and DON + 3-ADON + 15-ADON accounted for 1.32% and 12.21%, respectively. Only one maize sample did not contain all three mycotoxins. Our study shows the necessity of raising awareness of the co-occurrence of mycotoxin contamination in grains from China to protect consumers from the risk of exposure to DON and its derivatives. © 2020 by the authors.
语种：
英文
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蔬菜中农药残留分析检测技术的研究进展
作者：
杨明;涂凤琴;伊鋆;许晴;董秋花;...
期刊：
河南工业大学学报(自然科学版) ,2020年41(6):119-128 ISSN：1673-2383
作者机构：
[江小明; 杨明; 胡筱静; 涂凤琴; 杨永; 董秋花; 许晴; 伊鋆; 陈丹; 卢跃鹏] 武汉食品化妆品检验所;武汉轻工大学食品科学与工程学院;[宫智勇; 常超; 伍金娥] 武汉轻工大学
关键词：
农药残留;分析检测技术;蔬菜
摘要：
蔬菜中农药残留问题一直威胁着人们的健康安全,也严重阻碍了我国蔬菜出口贸易的发展,有效的分析检测技术是监测农药残留的关键之一。综述了近年来蔬菜中的农药残留现状,同时详细介绍了光谱法、免疫分析法、色谱法和色谱-质谱联用技术等分析检测技术的基本原理、特点,以及这些分析检测技术在蔬菜农药残留检测中的应用与研究进展,比较了各分析检测技术针对有机磷类、菊酯类、生长调节剂等农药的优势与不足,并对其发展进行展望,旨在为蔬菜中农药残留的检测提供理论和技术支持。
语种：
中文
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食品中农药残留分析前处理技术研究进展
作者：
杨明;伊鋆;王冬梅;胡筱静;王梦颖;...
期刊：
河南工业大学学报(自然科学版) ,2020年41(03):130-140 ISSN：1673-2383
作者机构：
武汉食品化妆品检验所,湖北武汉430012;武汉轻工大学食品科学与工程学院,湖北武汉430023;[江小明; 杨明; 李首道; 王冬梅; 胡筱静; 王梦颖; 涂凤琴; 董秋花; 许晴; 伊鋆; 卢跃鹏] 武汉食品化妆品检验所;[宫智勇; 常超; 伍金娥] 武汉轻工大学
关键词：
农药残留;前处理技术;食品;展望
摘要：
食品中残留的农药会威胁人们的健康,对其进行分析十分必要,也有助于对食品中的农药残留进行监管。然而食品种类繁多、基质复杂,必须通过有效的前处理技术进行提取和净化,以保障检测结果的准确性和可靠性。对近年来国内外应用于食品中农药残留分析的前处理技术（如固相萃取、固相微萃取、固相分散萃取、QuEChERS等）进行了综述,详细介绍了各类前处理技术的原理及应用,并且对比了这些技术在前处理有机磷类农药残留的优缺点,旨在为我国更好地开展农药残留检测工作提供理论依据和技术支撑,同时对其发展趋势进行展望。
语种：
中文
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The neurotoxicity of Nε-(carboxymethyl)lysine in food processing by a study based on animal and organotypic cell culture
作者：
Wu, Yang;Li, Yan;Zheng, Liangqing;Wang, Pengcheng;Liu, Yan;...
期刊：
Ecotoxicology and Environmental Safety ,2020年190:110077 ISSN：0147-6513
通讯作者：
Wu, Yongning;Gong, ZhiYong
作者机构：
[Liu, Yan; Wu, Yang; Zheng, Liangqing; Gong, ZhiYong; Wang, Pengcheng] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Li, Yan] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.;[Wu, Yongning] Minist Hlth, China Natl Ctr Food Safety Risk Assessment, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China.;[Gong, ZhiYong] Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构：
[Wu, Yongning] M;[Gong, ZhiYong] W;Minist Hlth, China Natl Ctr Food Safety Risk Assessment, Key Lab Food Safety Risk Assessment, Beijing 100021, Peoples R China.;Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
3D microvascular model;Nepsilon-(Carboxymethyl) lysine;Oxidative stress;p38MAPK-AP-1 signal pathway
摘要：
Nepsilon-(carboxymethyl)lysine (CML) is a potentially noxious compound that is causing widespread concern due to its use in various food products. In this study, we investigated CML neurotoxicity via an in vivo experiment with mice, and an in vitro experiment using a 3D microvascular network model (with human brain vascular endothelial cell and human astrocyte) that simulated the blood-brain barrier. We found that CML could induce cell survival status variations, and histopathological changes to the brain. In addition, CML increased levels of oxidative stress, prompted the protein expression of the receptor for advanced glycation end-products (RAGE). CML up-regulated both the gene expression of RAGE, the activating protein-1 (AP-1), the inflammatory cytokines Interleukin-6 (IL-6), vascular cell adhesion molecule1 (VCAM-1), monocyte chemotactic protein1 (MCP-1). We, therefore, postulated that CML has the potential to deleteriously affect the nervous system through oxidative stress and that activation of the p38 MAPK-AP-1 signaling pathway might be implicated in this pathological process.
语种：
英文
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Simultaneous and rapid determination of sesamin and sesamolin in sesame oils using excitation-emission matrix fluorescence coupled with self-weighted alternating trilinear decomposition
作者：
Liu, Yan* ;Wu, Qian;Xia, Zhenzhen;Wu, Yang;Li, Yan;...
期刊：
Journal of the Science of Food and Agriculture ,2020年100(12):4418-4424 ISSN：0022-5142
通讯作者：
Liu, Yan
作者机构：
[Liu, Yan; Wu, Yang; Gong, Zhiyong; Li, Yan; Wu, Qian] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.;[Liu, Yan; Wu, Yang; Gong, Zhiyong; Li, Yan] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Peoples R China.;[Liu, Yan; Wu, Yang; Gong, Zhiyong; Li, Yan] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Peoples R China.;[Xia, Zhenzhen] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan, Peoples R China.
通讯机构：
[Liu, Yan] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
1,3 dioxolane derivative;lignan;sesame seed oil;sesamin;sesamolin;chemistry;fluorescence;procedures;Sesamum;spectrofluorometry;Dioxoles;Fluorescence;Lignans;Sesame Oil;Sesamum;Spectrometry, Fluorescence
摘要：
BACKGROUND: Sesamin and sesamolin are two typical and important lignans isolated from sesame oil. Various studies have shown the bioactivity, physiological activity, and potential health benefits of the two components. In this study, a rapid method for the simultaneous determination of sesamin and sesamolin in sesame oils was proposed. The excitation-emission fluorescence spectra of the oils were obtained after a simple pretreatment, then self-weighted alternating trilinear decomposition was used to extract the quantitative information from the very overlapping spectra. RESULTS: It was found that reasonable quantification results could be obtained with the limits of detection for the two lignans. These limits were 0.05 mg/g and 0.24 mg/g, and the limits of quantitation were 0.14 mg/g and 0.74 mg/g, respectively. The average recoveries for sesamin and sesamolin were 99.05% and 94.97%. CONCLUSION: The results indicate that, with simple sample pretreatment, the application for combining excitation-emission fluorescence spectra and self-weighted alternating trilinear decomposition can be a useful and sensitive tool for the determination of lignans in sesame oil. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry
语种：
英文
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Determination of Trace Zearalenone and Its Metabolites in Human Serum by a High-Throughput UPLC-MS/MS Analysis
作者：
Sun, Danlei;Li, Chenglong;Zhou, Shuang* ;Zhao, Yunfeng;Gong, Yun Yun;...
期刊：
Applied Sciences-Basel ,2019年9(4):741 ISSN：2076-3417
通讯作者：
Zhou, Shuang;Wu, Yongning
作者机构：
[Sun, Danlei; Gong, Zhiyong] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Wu, Yongning; Zhao, Yunfeng; Zhou, Shuang; Wu, YN; Li, Chenglong] Natl Hlth Commiss, Key lab Food Safety Risk Assessment, China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China.;[Gong, Yun Yun] Univ Leeds, Sch Food Sci & Nutr, Leeds LS2 9JT, W Yorkshire, England.
通讯机构：
[Zhou, S; Wu, YN] N;Natl Hlth Commiss, Key lab Food Safety Risk Assessment, China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China.
关键词：
96-well μElution SPE;Metabolites;Serum;UPLC-MS/MS;Zearalenone
摘要：
This paper described an improved method for high-throughput and sensitive determination of zearalenone and its five metabolites (zearalanone, α-zearalenol, β-zearalenol, α-zearalanol and β-zearalanol) in human serum. Serum samples were measured both before and after enzyme hydrolysis to assess the free and total amount of each compound by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in multi reaction monitoring (MRM) mode following off-line 96-well μElution solid-phase extraction (SPE). All the analytes were completely separated on a C18 column within 6 min. It enabled multi-sample preparation at the same time eliminating tedious evaporation and reconstitution steps, allowing 96 (one plate) samples to be processed and analyzed within 24 h. Using an isotope labelled internal standard ( 13 C-ZEN), high recoveries were achieved for all the compounds in the range 91.6%-119.5%, with intra-day and inter-day relative standard deviations (RSDs) of less than 8%. The limits of detection (LOD) and the limits of quantification (LOQ) were 0.02-0.06 ng mL -1 (0.6-2 fmol) and 0.1-0.2 ng mL -1 (3-6 fmol), respectively, demonstrating a notable enhancement in sensitivity compared to the existing methods. The validated method was applied to the analysis of paired urine and serum samples collected from 125 healthy individuals in Henan Province, locating in the middle area of China. ZEN metabolites in human serum were significantly lower than those in urine. Only one serum sample was positive for ZEN after enzyme digestion, whereas at least one of ZEN biomarkers was detected in 75.2% of the paired urine samples. Some comparison and discussion were also included in this paper. © 2019 by the authors.
语种：
英文
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Functional human 3D microvascular networks on a chip to study the cytocompatibility of alpha-MnO2 nanowire
作者：
Li, Yan;Wang, Pengcheng;Gong, Zhiyong;Wu, Yang* ;Yang, Xu*
期刊：
Ferroelectrics ,2019年546(1):13-24 ISSN：0015-0193
通讯作者：
Wu, Yang;Yang, Xu
作者机构：
[Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Wu, Yang; Gong, Zhiyong] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wu, Yang] W;[Yang, Xu] C;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 9th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN)
会议时间：
AUG 17-20, 2018
会议地点：
Hohhot, PEOPLES R CHINA
会议主办单位：
[Li, Yan;Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Gong, Zhiyong;Wu, Yang] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
关键词：
3D cell cuture;MnO2 nanowires;oxidative stress;vascular toxicity
摘要：
A 3D functional human microvascular network in a microfluidic device was developed in this study. The established model enables endotheilal cells to form vessel-like microtissues and behave physiological functions which are closer to cells in human blood vessels. It provides a possible bridge for how the research outcomes translate to humans. The ɑ-MnO2 nanowires, which were successfully synthesized using the hydrothermal approach. The vascular toxicity was evaluated based on this organotypic 3D microvessel model. Our results demonstrated that ɑ-MnO2 nanowires can induce cytotoxicity in dose-dependently. Because the accumulation of reactive oxygen species (ROS) and depletion of glutathione (GSH) are observed in the nanowire-treated cells. In addition, intravascular accumulation of ɑ-MnO2 nanowires could induce endothelial dysfunction by oxidative stress. This is expressed in disorder of NO expression and IL-6 up-regulation. Based on these results, we concluded that a mechanism for ɑ-MnO2 nanowire-induced vascular toxicity, which involves the accumulation of ROS, up-regulation of oxidative stress and inflammatory factors. This investigation may provide a fundamental insight to understand the nanotoxicity of wire-shaped nanomaterials. © 2019, © 2019 Taylor & Francis Group, LLC.
语种：
英文
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The intervention mechanism of folic acid for benzo(a)pyrene toxic effects in vitro and in vivo
作者：
Wang, Pengcheng;Chen, Yuwei;Wang, Lei;Wu, Yang;Wang, Lichun;...
期刊：
EUROPEAN JOURNAL OF CANCER PREVENTION ,2019年28(4):355-364 ISSN：0959-8278
通讯作者：
Gong, Zhiyong
作者机构：
[Chen, Yuwei; Wu, Yang; Gong, Zhiyong; Wang, Lei; Wang, Lichun; Wang, Pengcheng] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Wu, Yongning] China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing, Peoples R China.;[Gong, Zhiyong] WuHan Polytech Univ Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Gong, Zhiyong] W;WuHan Polytech Univ Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
benzo(a)pyrene;cell cycle;folic acid;interference effect
摘要：
Folic acid is a necessary micronutrient for normal human growth and development. Benzo(a)pyrene (BaP) is a ubiquitously distributed environmental pollutant and its metabolite, benzo(a)pyrene-diol-epoxide, is known to exert a strong teratogenic and carcinogenic effect on the body’s tissues and cells. The aim of this study was to investigate the mechanism by which folic acid can inhibit the toxic effects of BaP both in vivo and in vitro. We measured changes in 16HBE cell activity affected by the intervention of folic acid on BaP using the cell counting kit-8 assay and that of cell cycle distribution by flow cytometry. At the same time, we assessed the xeroderma pigmentosum group A, xeroderma pigmentosum group C, excision repair cross complementation group 1, cyclinD1, and CKD4 mRNAs, and their related protein expression both in mouse lung tissue and in 16HBE cells. In conclusion, the mechanisms by which this effect is mediated were not entirely elucidated by our study, possibly because folic acid antagonizes the toxic effects of BaP by upregulating the levels of excision repair cross complementation group 1, xeroderma pigmentosum group A, and xeroderma pigmentosum group C gene expression to improve the rate of DNA repair, in turn accelerating the speed of repair for DNA damage caused by BaP. Meanwhile, folic acid could restrain BaP-induced cyclinD1 protein expression, which could help cells return to their normal cell cycle.
语种：
英文
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体外消化/Caco-2细胞模型评价不同食品中高氯酸盐的生物利用率
作者：
田一媚;许浩;刘孙燕;方敏;吴永宁;...
作者机构：
武汉轻工大学食品科学与工程学院中国食品安全风险评估中心
会议名称：
中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛
会议时间：
2019-11-13
会议地点：
中国湖北武汉
会议论文集名称：
中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛论文摘要集
关键词：
高氯酸盐;食品;体外消化/Caco-2细胞模型;生物可及性;转运;生物利用率
摘要：
目的:通过研究不同食品中高氯酸盐的生物可及性和生物利用率,以进行高氯酸盐在人群中合理的健康风险评估。方法:采用RIVM体外消化/Caco-2细胞模型法研究了不同食品中高氯酸盐的生物可及性、转运吸收机制和生物利用率。结果:生菜、大米和奶粉的生物可及性大小依次为,（68.23±2.06）%、（68.27±3.45）%和（58.70±2.86）%,均高于高氯酸盐对照组（56.40±2.82）%。各食品中高氯酸盐在胃肠道吸收的大小关系依次为:大米组>生菜组>奶粉组>对照组。生菜、大米、奶粉和对照组中高氯酸盐的外排率依次为1.29、0.74、0.84、1.57。生菜、大米和奶粉中高氯酸盐的体外生物利用率依次为:（34 5±1.0）%、（37.9±1.9）%和（26.2±1.3）%,均高于高氯酸钠对照组（23.6±1.2）%。结论:高氯酸盐在模型中的转运途径可能为被动扩散。食品基质的存在,不同程度地促进了机体对高氯酸盐的吸收利用,提高了高氯酸盐的生物利用率。
语种：
中文
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小麦和玉米中呕吐毒素及衍生物暴露水平及毒性作用研究
作者：
尹晓瑶;严翩翩;董晶晶;宋婉莹;方敏;...
作者机构：
武汉轻工大学食品科学与工程学院国家食品安全风险评估中心
会议名称：
中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛
会议时间：
2019-11-13
会议地点：
中国湖北武汉
会议论文集名称：
中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛论文摘要集
关键词：
呕吐毒素;衍生物;细胞
摘要：
目的:目前我国食品安全标准未将3-ADON、15-ADON纳入标准,本研究旨在为我国粮食中DON及其衍生物限量标准制定提供更加科学的依据。方法:采用UPLC-MS/MS串联检测方法,对2017年我国579份小麦籽粒样品和606份玉米样品进行分析;并通过不同浓度（0.2,0.5,1,2,5,10μmol/L）DON、3-ADON、15-ADON作用Caco-2细胞24,48,72 h,用CCK-8法测定细胞活性。结果:我国粮食主产区小麦、玉米总样本中呕吐毒素及衍生物总含量（DONs）范围为LOQ-6436.11μg/kg,检出率为99.92%,总平均含量为232.82μg/kg;在采集的2017年全国样品中DON检出率高达99.92%、3-ADON检出率为8.95%、15-ADON检出率为39.32%。全国小麦和玉米总样本中DON、15-ADON、DONs超标率分别为3.29%、0.25%、5.06%。暴露24,48,72 h后,DON实验组细胞活性分别由101.90%下降至53.91%、28.49%、13.07%;3-ADON由111.15%下降至60.49%、33.47%、22.33%;15-ADON由97.99%下降至48.86%、33.47%、13.07%。结论:通过比较不同省份地区小麦、玉米中呕吐毒素及衍生物含量,发现南方地区小麦中呕吐毒素及衍生物含量明显高于北方地区,中部浓度最高。DON、3-ADON、15-ADON的IC50范围分别为0.497～0.596μmol/L、0.770～0.998μmol/L、0.476～0.560μmol/L,且毒性为15-ADON≥DON>3-ADON。
语种：
中文
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游离态羧甲基赖氨酸对秀丽隐杆线虫的毒性作用
作者：
许浩;张虎;姚丽云;池晟;方敏;...
期刊：
武汉轻工大学学报 ,2019年38(03):4-12+36 ISSN：2095-7386
作者机构：
武汉轻工大学食品科学与工程学院;[张虎] 大宗粮油精深加工教育部重点实验室;[许浩] 武汉轻工大学
关键词：
羧甲基赖氨酸(CML);秀丽隐杆线虫;寿命;作用机制
摘要：
羧甲基赖氨酸(CML)是食品加工中经美拉德反应由蛋白质与糖类通过非酶催化反应产生的一种晚期糖基化终末产物,是一种新型食品潜在有害物质。本文通过研究外源游离性CML对秀丽隐杆线虫寿命、子代数量、热环境中线虫存活时间的影响,探讨其可能的毒性机制。结果表明,外源游离性CML导致线虫产卵期缩短,寿命试验以及热刺激下存活的线虫数量呈现出剂量-反应关系。影响了TOR信号通路的Skn-1基因和生殖系统信号通路中的hsf-1基因,提高线虫的热环境阻力的抵抗力并延长其寿命。高浓度的CML降低了线虫的中DAF-16的表达水平从而影响其寿命,抑制线虫体内Folt-1基因的表达,从而减少其子代线虫的数量。
语种：
中文
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Studies on mechanism of free Nε-(carboxymethyl)lysine-induced toxic injury in mice
作者：
Wang, Yi-Xin;Xu, Hao;Liu, Xin;Liu, Liang;Wu, Yong-Ning;...
期刊：
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY ,2019年33(7):e22322 ISSN：1095-6670
通讯作者：
Gong, Zhi-Yong
作者机构：
[Wang, Yi-Xin; Xu, Hao; Liu, Liang; Gong, Zhi-Yong; Liu, Xin] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan, Hubei, Peoples R China.;[Wu, Yong-Ning] Minist Hlth, China Natl Ctr Food Safety Risk Assessment, Key Lab Food Safety Risk Assessment, Beijing, Peoples R China.;[Gong, Zhi-Yong] Wuhan Polytech Univ, Coll Food Sci & Engn, 68 XueFuNan Rd, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Gong, Zhi-Yong] W;Wuhan Polytech Univ, Coll Food Sci & Engn, 68 XueFuNan Rd, Wuhan 430023, Hubei, Peoples R China.
关键词：
advanced glycation end product receptor;lysine derivative;messenger RNA;n epsilon(carboxymethyl)lysine;transforming growth factor beta;tumor necrosis factor;unclassified drug;vascular cell adhesion molecule 1;advanced glycation end product receptor;Ager protein, mouse;lysine;N(6)-carboxymethyllysine;transforming growth factor beta;tumor necrosis factor;vascular cell adhesion molecule 1;animal experiment;animal tissue;Article;controlled study;drug intoxication;female;immunohistochemistry;kidney injury;kidney tissue;liver injury;liver tissue;male;mouse;mRNA expression level;nonhuman;oxidative stress;real time polymerase chain reaction;Western blotting;analogs and derivatives;animal;chemically induced;drug effect;inflammation;kidney;liver;metabolism;pathology;Animals;Female;Inflammation;Kidney;Liver;Lysine;Male;Mice;Oxidative Stress;Receptor for Advanced Glycation End Products;Transforming Growth Factor beta;Tumor Necrosis Factor-alpha;Vascular Cell Adhesion Molecule-1
摘要：
Nε-(carboxymethyl)lysine (CML), which is a compound produced when food is processed, has aroused concern in recent years because of its potentially dangerous effects. This study aimed to investigate the mechanism of free CML-induced toxic injury in mice. The inflammatory cytokine tumor necrosis factor-α, transforming growth factor-β, vascular cell adhesion molecule-1 mRNA expression levels of CML-infected mice liver and kidney tissues significantly increased. While CML receptor—receptor for advanced glycation end products (RAGE) protein expression in male mice liver tissue had a more significant change than the control group, there was no significant difference in other dose groups compared with the control group. In conclusion, the foodborne free CML can be induced by oxidative stress and immune response to liver and kidney tissue injury in mice. Additionally, the free CML may also bind to RAGE, which activates the downstream inflammatory pathway. © 2019 Wiley Periodicals, Inc.
语种：
英文
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水浸法削减大米粉中镉的工艺优化及对其品质影响
作者：
陈雨薇;王蕾;吴永宁;宫智勇
期刊：
食品科学 ,2019年40(10):272-278 ISSN：1002-6630
作者机构：
武汉轻工大学食品科学与工程学院, 湖北大宗粮油精深加工省部共建教育部重点实验室, 湖北, 武汉, 430030;国家食品安全风险评估中心重点实验室, 国家食品安全风险评估中心重点实验室, 北京, 100032;[陈雨薇; 宫智勇; 王蕾] 武汉轻工大学;[吴永宁] 国家食品安全风险评估中心
关键词：
水浸;镉;大米粉;削减;品质
摘要：
采用响应面法优化水浸法削减镉污染的大米粉中镉的工艺条件,在最优条件下测定水浸法对大米粉营养成分的影响,并用扫描电镜、差示扫描量热仪、X射线衍射、红外光谱等方法分别研究水浸法对大米粉形态、热力学特征、淀粉晶体结构及官能团的影响。结果表明:水浸法削减大米粉中镉的最佳条件为水浸温度42℃、料液比1∶3(g/m L)、水浸时间47 h,此时镉含量为(0.459±0.006)mg/kg,大米粉中的镉去除率达到(61.75±4.31)%,处理后大米粉中镉含量为(0.176±0.003)mg/kg。在最佳工艺条件下,大米粉中的蛋白质含量显著降低(P<0.05),脂肪、淀粉、灰分也略有下降(P<0.05)。大米粉水浸后糊化起始温度降低,而峰值温度变化很小,说明大米粉晶体片层被破坏,稳定性降低,大米粉中直链淀粉含量可能升高,但水浸处理并没有破坏米粉内淀粉粒晶体成分。水浸大米粉面间距有略微的降低,说明水浸可能导致大米粉产品较原料产品的硬度、咀嚼度、回弹性、黏聚性下降。大米粉在水浸过程中并没有产生新的化学键或基团。
语种：
中文
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氯化镉与镉污染大米中镉在大鼠体内吸收和分布比较
作者：
吴倩;王蕾;方敏;吴永宁;宫智勇
作者机构：
武汉轻工大学食品科学与工程学院大宗粮油精深加工教育部重点实验室国家食品安全风险评估中心
会议名称：
中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛
会议时间：
2019-11-13
会议地点：
中国湖北武汉
会议论文集名称：
中国食品科学技术学会第十六届年会暨第十届中美食品业高层论坛论文摘要集
关键词：
镉污染大米;吸收;分布;镉
摘要：
目的:本研究旨在探讨氯化镉和镉污染大米中镉在体内吸收和分布的差异,为阐明大米中镉可能存在的健康风险提供依据。方法:采用SD大鼠模型,研究镉在镉污染大米和氯化镉（CdCl2）中的代谢、吸收和分布。研究比较了Cd～（2+）和大米中Cd的积累情况。结果:CdCl2组和Cd大米组的大鼠分别单次灌胃氯化镉和镉污染大米浑浊悬液（Cd的摄入量均为10μg/kg bw）,CdCl2组和Cd大米组的大鼠血液中Cd浓度在2 h达到最高值,16 h后下降到与空白对照组相同的浓度。经过21天的持续灌胃后,每组动物的血、心、脾、肺中Cd含量无明显差异。与对照组相比,镉大米组大鼠肝脏和肾脏中镉的累积量显著增加118.78%和192.90%。与CdCl2组相比,Cd大米组大鼠肝脏和肾脏的Cd含量分别比CdCl2组高31.69%和42.91%。结论:CdCl2组与Cd大米组的大鼠血液中Cd代谢规律基本一致。低剂量Cd摄入21 d后,Cd在水稻中主要积聚在实验大鼠的肾脏和肝脏中,而在血液、心脏、脾脏和肺中仅略有积聚。大米中福在大鼠体内的累积量大于无机镉（CdCl2）。
语种：
中文
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QuEChERS-HPLC-MS/MS测定蔬菜中29种农药残留
作者：
杨明;伊鋆;许晴;董秋花;李首道;...
期刊：
中国酿造 ,2019年38(09):190-195 ISSN：0254-5071
作者机构：
[江小明; 杨明; 李首道; 管卓龙; 王梦颖; 涂凤琴; 董秋花; 何丽姗; 许晴; 伊鋆; 陈丹; 卢跃鹏] 武汉食品化妆品检验所;武汉轻工大学食品科学与工程学院;[宫智勇] 武汉轻工大学
关键词：
QuEChERS预处理法;高效液相色谱-串联质谱法;农药残留;蔬菜
摘要：
建立了QuEChERS-高效液相色谱-串联质谱(HPLC-MS/MS)同时快速测定蔬菜中29种农药残留的分析方法。样品以乙腈提取,氯化钠盐析,以十八烷基硅烷键合硅胶(C18)和N-丙基乙二胺(PSA)净化,以WatersXBridgeC18色谱柱分离,电喷雾正离子扫描,多反应监测(MRM)模式检测,基质匹配标准溶液外标法定量。在此优化条件下,29种农药分子在不同加标水平下的平均回收率为64.62%~123.55%,相对标准偏差(RSD)为0.7%~14.3%,方法定量限(LOQ)为0.40~8.00μg/kg。该方法简便快速、灵敏度高、重现性好,能满足蔬菜中农药残留的快速检测要求。
语种：
中文
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A molecularly imprinted polymers/carbon dots-grafted paper sensor for 3-monochloropropane-1,2-diol determination
作者：
Fang, Min* ;Zhou, Lv;Zhang, Hu;Liu, Liang;Gong, Zhi-Yong*
期刊：
Food Chemistry ,2019年274:156-161 ISSN：0308-8146
通讯作者：
Fang, Min;Gong, Zhi-Yong
作者机构：
[Zhou, Lv; Zhang, Hu; Liu, Liang; Gong, Zhi-Yong; Fang, Min] Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhou, Lv; Zhang, Hu; Liu, Liang; Gong, Zhi-Yong; Fang, Min] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan, Hubei, Peoples R China.
通讯机构：
[Fang, M; Gong, ZY] W;Wuhan Polytech Univ, Inst Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
关键词：
3-Chloro-1,2-propandiol;Carbon dot;Fluorescence;Molecularly imprinted polymers (MIPs);Paper-based sensor
摘要：
3-Monochloropropane-1,2-diol (3-MCPD) is a common food processing contaminant and a simple, rapid, sensitive and low cost monitoring technology is needed due to its potential carcinogenic nature. Carbon dots directly intercepted on filter paper provide high fluorescence intensity and can be adapted for use as a sensor. We synthesized a carbon dot-filter paper in combination with a molecularly imprinted polymeric film to extract 3-MCPD from samples. This grafted paper-based sensor exhibited a high adsorption capacity (68.97mgg(-1)), an excellent selectivity (imprinting factor=4.5) and a low detection limit (0.6ngmL(-1)). Recoveries ranged from 97.2% to 105.3% with relative standard deviations <5.6%. The results obtained using this method were linearly correlated to those of the classic GC-MS method (r=0.998). Based on the Chinese National Standard, this study provides a novel and powerful platform for the simple, rapid, sensitive and on-site analysis of 3-MCPD in soy sauce.
语种：
英文
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Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip
作者：
Li, Yan;Hu, Chuanlin;Wang, Pengcheng;Liu, Yan;Wang, Luyang;...
期刊：
Journal of Nanobiotechnology ,2019年17(1):1-17 ISSN：1477-3155
通讯作者：
Wu, Yang;Mak, Michael
作者机构：
[Liu, Yan; Gong, Zhiyong; Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Coll Food Sci & Engn, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.;[Wu, Yang] Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.;[Wang, Luyang] Tsinghua Univ, Dept Bldg Sci, Beijing 100084, Peoples R China.;[Pi, Qingmeng] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA.
通讯机构：
[Wu, Yang] W;[Mak, Michael] Y;Wuhan Polytech Univ, Coll Food Sci & Engn, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Hubei, Peoples R China.;Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.
关键词：
Indoor particulate matter;Oxidative stress;Inflammation;Promote coagulation;3D human microvessel
摘要：
Background: A growing body of evidence shows that indoor concentrations of airborne particles are often higher than is typically encountered outdoors. Since exposure to indoor PM2.5 is thought to be associated with cardiovascular disease, the health impacts of indoor air pollution need to be explored. Based on animal models, ambient particulate matter has been proved to promote coagulation which is very likely involved in the pathogenic development of cardiovascular disease. However, animal models are insufficient to predict what will happen with any certainty in humans. For this reason, the precise pathogenic mechanisms behind the development of cardiovascular disease in humans have not yet been determined. Results: We generated a 3D functional human microvascular network in a microfluidic device. This model enables human vascular endothelial cells to form tissue-like microvessels that behave very similarly to human blood vessels. The perfusable microvasculature allows the delivery of particles introduced into these generated human-like microvessels to follow the fluid flow. This exposure path effectively simulates the dynamic movement of airborne nanoscale particles (ANPs) within human vessels. In this study, we first identified the existence of ANPs in indoor air pollution. We then showed that ANPs could activate endothelial cells via ROS induced inflammation, and further resulted in abnormal expression of the coagulation factors (TF, TM and t-PA) involved in coagulation cascades. In addition, we found that a protein could cover ANPs, and this biointeraction could interfere with heparan sulfate (HS). Human organotypic 3D microvessel models provide a bridge for how research outcomes can translate to humans. Conclusions: The 3D human microvessel model was used to determine the physiological responses of human vessels to ANP stimulation. Based on the obtained data, we concluded that ANPs not only disrupts normal coagulation functions, but also act directly on anticoagulant factors in human vessels. These experimental observations provide a potential biological explanation for the epidemiologically established link between ANPs and coagulation abnormality. This organ-on-chip model may provide a bridge from in vitro results to human responses. © 2019 The Author(s).
语种：
英文
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